Claims
- 1. An isolated nucleic acid encoding the human metabotropic glutamate receptor which comprises the amino acid sequence designated as SEQ ID NO:2.
- 2. A composition comprising an isolated nucleic acid containing a sequence encoding a human glutamate receptor as claimed in claim 1, wherein said sequence is selected from the group consisting of:a) SEQ ID NO:1 b) SEQ ID NO:3 c) a nucleic acid compound complementary to (a) or (b); and d) a fragment of (a), (b), or (c) that is at least 18 base pairs in length and which will selectively hybridize to human genomic DNA encoding a human metabotropic glutamate receptor.
- 3. The composition of claim 2 wherein the isolated nucleic acid is deoxyribonucleic acid.
- 4. The composition of claim 3 which is (a) or a sequence complementary to (a).
- 5. The composition of claim 3 which is pGTh/HmGluR3.
- 6. The composition of claim 2 wherein the isolated nucleic acid is ribonucleic acid.
- 7. The composition of claim 6 which is (b) or a fragment thereof.
- 8. An expression vector capable of producing a human metabotropic glutamate receptor or a fragment thereof in a host cell which comprises a nucleic acid as claimed in claim 2 in combination with regulatory elements necessary for expression of the nucleic acid in the host cell.
- 9. The expression vector of claim 8 for use in a host cell wherein the host cell is a mammalian cell line.
- 10. The expression vector of claim 9 which comprises a BK virus enhancer.
- 11. The expression vector of claim 10 which further comprises an adenovirus late promoter.
- 12. The expression vector of claim 11 wherein the mammalian cell line is the RGT cell line.
- 13. A transfected host cell harboring an expression vector as claimed in claim 8.
- 14. A transfected host cell as claimed in claim 13 which is a transfected mammalian cell line.
- 15. A transfected host cell as claimed in claim 14 which is RGT-18 transfected with pGTh/HmGluR3.
- 16. An isolated human metabotropic glutamate receptor which comprises the amino acid sequence designated herein SEQ ID NO:2.
- 17. A method for producing mGlur3 protein comprising the steps of:a) expressing a gene sequence identified herein as SEQ ID NO:1 in a suitable host cell such that a recombinant protein comprising SEQ ID NO:2 is expressed; and b) purifying said recombinant protein by any suitable method.
- 18. An mGlur3 protein produced by the method of claim 17.
Parent Case Info
This application claims benefit under Title 35, United States Code, § 119(e) of U.S. provisional patent application No. 60/008,959, filed Dec. 20, 1995.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5521297 |
Daggett et al. |
May 1996 |
A |
Non-Patent Literature Citations (2)
Entry |
Amara, S.G., “A tale of two families”, Nature 360:420-421, Dec. 3, 1992.* |
Yasuto Tanabe et al. “A Family of Metabotropic Glutamate Receptors” Neuron 8:169-179 (Jan., 1992). |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/008959 |
Dec 1995 |
US |