Patent Application
20230349005
This sequence listing is created on 08/30/2022 with the file name “P22006TWTOU_ST25_SF22_0003 UP_0830” and file size 33407 bytes; the entire contents of which are hereby incorporated by reference.
The present invention relates to an exogenous biomolecular tracing method and system thereof for aquatic creatures and products. Particularly, the present invention relates to the exogenous biomolecular tracing method and system thereof with nucleotide markers for aquatic creatures and products.
By way of example, Taiwanese Patent Application Publication No. TW-200911131, entitled “Feed composition for aquatic organisms,” discloses a feed composition including a protein, an additional protein, a lipid, an additional lipid, a carbohydrate and water which are formed particles ranging between 1 to 100 micrometers.
The sources of protein are provided from fish meat or high-quality fish meat, the lipid is provided from fish oils, the additional lipid is provided from cholesterol, vegetable oils or lecithin, with the lecithin is selected from egg yolk or soybeans. The additional lipid includes fish hydrosylate, hill, cuttlefishes or extracts of yeasts.
The feed composition further includes at least one mineral substance and at least one vitamin, with the mineral substance including trace elements and calcium salts, the calcium salt selected from calcium carbonate or calcium phosphate and the vitamins including vitamin A, vitamin B complex, vitamin C and vitamin E.
Further, another Taiwanese Patent Publication No. TW-I656835, entitled “Breeding and baiting system and method thereof for fry of aquatic creatures,” discloses a breeding and baiting system and method thereof, including a breeding tank, a water inlet, a water supply conduit and a water outlet.
The breeding tank includes an inner space portion and an opening portion, with disposing the water inlet and the water outlet at a first predetermined position and a second predetermined position of the inner space portion, with connecting the water supply conduit with the inner space portion or the water inlet to continuously or intermittently supply a predetermined water jet flow.
In breeding and baiting operation, the predetermined water jet flow is applied to drive at least one bait material to thereby form a driven dynamic bait so that a plurality of fry can chase the driven dynamic bait in the breeding tank.
Further, another Taiwanese Patent Publication No. TW-I660669, entitled “Artificial sausage-casing type feed, baiting device and method thereof for aquatic creatures,” discloses a baiting device, including an artificial sausage-type feed, a baiting support frame, a floating device and an operation member.
The artificial sausage-casing-type feed has a rope member, at least one insert member and at least one casing in which to form an inner space to enclose a bait filling. An end of the rope member connects with a connection end of insert member while an insert end of insert member inserts into the filled casing to form the artificial sausage-casing-type feed.
The baiting support frame has a fixing bar on which to connect the rope member of the artificial sausage-casing-type feed. The floating device further connects with the fixing bar of the baiting support frame for providing a floating force thereon.
The operating member has a trolling rope member to connect the floating device to form the baiting device which is provided under a water surface. In a baiting operation the operation member can be manually or automatically controlled to troll the baiting support frame to provide the artificial sausage-type feed to at least one aquatic creature for chasing, catching and eating it.
Further, another Taiwanese Patent Publication No. TW-I695679, entitled “Auxiliary light system and method thereof for baiting aquatic creatures,” discloses an auxiliary light system, including a baiting light device or a plurality of baiting light devices.
The baiting light device can be operated to project a movable light beam or a plurality of movable light beams to form a dynamic light spot or a plurality of dynamic light spots under water surface. In a baiting operation, aquatic creatures can chase, catch and bite the dynamic light spots for auxiliary baiting operation.
Further, another Taiwanese Patent Publication No. TW-1706723, entitled “Stress relief formula for fry or adult of cephalopods of aquatic creatures,” discloses a stress relief formula, including at least one cephalopod extract and a water solution.
The cephalopod extract is extracted from at least one cephalopod and the water solution is added to the cephalopod extract to produce a cephalopod stress relief formula. The cephalopod stress relief formula can be applied to add to a cultural water body for protecting fry or adult of cephalopods therein.
Further, another UK Patent Application Publication No. GB-2395414, entitled “Gel feed for fish,” discloses a gel feed. The gel feed is an animal feed composition (or aquatic animal composition) which includes at least one dry powdered diet for forming the gel feed.
When the dry powdered diet selectively mixed with wet feed and/or with water, the dry powdered diet can be formed as a gel. The animal feed composition further includes an algae material performed as a binder therein.
Furthermore, the animal feed composition may optionally include a liquid component.
Further, another Chinese Patent Application Publication No. CN-110964845, entitled “Method capable of tracing maize mixed pollination hybrid seed sources and InDel molecular marker,” discloses a method of tracing maize mixed pollination hybrid seed sources and an InDel molecular marker.
The method comprises the steps: (1) extracting DNA of all male parents, female parents and mixed pollination hybrid seed leaves to be detected; (2) screening molecular markers capable of establishing a fingerprint spectrum by utilizing the DNA of the male parent and the female parent.
The method further comprises the steps: (3) performing PCR amplification on the DNA template in step (1) by using the screened molecular markers, and recording genotypes of the male parents, the female parents and the mixed pollination hybrid seeds to be detected; (4) deriving all possible hybrid genotypes according to the obtained genotypes of the male parents and the female parents.
The method further comprises the steps: (5) comparing the genotypes of the mixed pollination hybrid seeds to be detected obtained in step (3) with the genotypes of all the hybrid seeds derived in step (4), and recording the number of sites with the same genotype.
The method further comprises the step: If a certain mixed pollination hybrid seed to be detected and a certain derived hybrid seed have the maximum site number of the same genotype, the parent of the derived hybrid seed is derived as the parent source of the mixed pollination hybrid seeds to be detected.
Further, another PCT (Patent Cooperation Treaty) Patent Application Publication No. WO-2011/108062, entitled “Method for labeling and identifying fishery fish and shellfish by mitochondrial DNA variable region base sequence,” discloses a method for labeling and identifying fishery fish and shellfish by mitochondrial DNA variable region base sequence.
The method for labeling and identification of fishery fish and shellfish is a simple and accurate method for labeling and identification of fish and shellfish, particularly fishery fish and shellfish derived from a specific production area, or superior fishery eggs and fry having one or a plurality of superior characterizing features, or a specific type of fishery fish and shellfish.
The method for labeling and identification of fishery fish and shellfish is a method for labeling and determining or tracking the profile of a single fishery fish or shellfish, an individual school of fishery fish or shellfish, a portion thereof, or the processed product thereof, by using the D loop base sequence of mitochondrial DNA. The method is also provided to label fishery fish and shellfish, particularly fertilized eggs, juvenile fish, or fingerlings.
However, there is a need of improving the conventional method for labeling and identification of fishery fish and shellfish performed as an identification of unique nucleotide marker for aquatic creatures and products. The above-mentioned patents and patent application publications are incorporated herein by reference for purposes including, but not limited to, indicating the background of the present invention and illustrating the situation of the art.
The primary objective of this invention is to provide an exogenous biomolecular tracing method and system thereof for aquatic creatures and products thereof. A nucleotide marker is extracted from a marker source organism, with further combining a basic material with the nucleotide marker to form a nucleotide marker-contained material. An aquatic creature or an aquatic product is applied to exogenously combine with the nucleotide marker-contained material to form an exogenously-marked aquatic creature or an exogenously-marked aquatic product. In tracing operation, at least one primer pair is provided to identify a DNA sample received from the exogenously-marked aquatic creature or the exogenously-marked aquatic product in an exogenous biomolecular tracing procedure to obtain an exogenously-marked identification result. Advantageously, the exogenous biomolecular tracing method and system of the present invention is successful in tracing original aquatic creatures and products thereof.
The exogenous biomolecular tracing system in accordance with an aspect of the present invention includes:
In a separate aspect of the present invention, the nucleotide marker is selected from an insect extract, a plant extract, a microorganism extract or combinations thereof.
In a further separate aspect of the present invention, the basic material is selected from a solution, a powder, a fragment, a solid or combinations thereof.
In yet a further separate aspect of the present invention, the aquatic creature or the aquatic product is selected from fishes, shrimps, shellfishes, algae or combinations thereof.
In yet a further separate aspect of the present invention, the aquatic creature or the aquatic product is selected from edible aquatic creatures, ornamental aquatic creatures, live aquatic creatures, frozen aquatic creatures, aquatic fry or aquatic baits.
The exogenous biomolecular tracing method in accordance with an aspect of the present invention includes:
In a separate aspect of the present invention, the nucleotide marker is selected from a DNA extract, a DNA extract liquid, a DNA extract powder, a DNA extract-related material or combinations thereof.
In a further separate aspect of the present invention, exogenously combining the nucleotide marker-contained material with the aquatic creature or the aquatic product includes a feeding process, a dipping process, a painting process, a spraying process or combinations thereof.
In yet a further separate aspect of the present invention, the at least one primer pair is applied to execute a test of polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP).
In yet a further separate aspect of the present invention, the exogenously-marked identification result includes a combination set of codes or code forms for tracing the exogenously-marked aquatic creature or the exogenously-marked aquatic product.
The exogenous biomolecular tracing method in accordance with another aspect of the present invention includes:
In a separate aspect of the present invention, the first or second nucleotide marker is selected from a DNA extract, a DNA extract liquid, a DNA extract powder, a DNA extract-related material or combinations thereof.
In a further separate aspect of the present invention, exogenously combining the first or second nucleotide marker-contained material with the aquatic creature or the aquatic product includes a feeding process, a dipping process, a painting process, a spraying process or combinations thereof.
In yet a further separate aspect of the present invention, the at least one primer pair is applied to execute a test of polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP).
In yet a further separate aspect of the present invention, the exogenously-marked identification result includes a combination set of codes or code forms for tracing the second exogenously-marked aquatic creature or the second exogenously-marked aquatic product.
Further scope of the applicability of the present invention will become apparent from the detailed description given hereinafter. However, it should be understood that the detailed description and specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only, since various will become apparent to those skilled in the art from this detailed description.
The present invention will become more fully understood from the detailed description given hereinbelow and the accompanying drawings which are given by way of illustration only, and thus are not limitative of the present invention, and wherein:
It is noted that an exogenous biomolecular tracing method (or usage) and system (e.g., information system) thereof for aquatic creatures and products thereof in accordance with the preferred embodiment of the present invention can be applicable to various aquatic creature baiting systems, various aquatic fry or creature breeding systems, various aquatic creature display systems, various algae (or microalgae) cultivation systems or various algae display systems, which are not limitative of the present invention.
For instance, an exogenous biomolecular tracing method (or usage) and system (e.g., information system) thereof for aquatic creatures and products thereof in accordance with the preferred embodiment of the present invention can be also applicable to various aquatic creature feeds, e.g., various crustacean feeds (including crabs, crab larvae, shrimps, shrimp larvae, lobsters, lobster larvae, krill, krill larvae etc.), various fish (fry) feeds, various animal feeds or various algae feeds (including algae fish feeds or Taiwan Tilapia feeds), which are not limitative of the present invention.
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Although the invention has been described in detail with reference to its presently preferred embodiment, it will be understood by one of ordinary skills in the art that various modifications can be made without departing from the spirit and the scope of the invention, as set forth in the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 111116589 | May 2022 | TW | national |
