Claims
- 1. An isolated polypeptide having expansin activity comprising an amino acid sequence selected from the group consisting of:
(a) a polypeptide sequence comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (b) a polypeptide having at least 90% identity to the sequences of (a), wherein said polypeptide retains expansin activity; (c) a polypeptide encoded by a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; and, (d) a polypeptide sequence comprising at least 40 consecutive amino acids of SEQ ID NO: 2 or SEQ ID NO: 4, wherein said polypeptide retains expansin activity.
- 2. An isolated nucleotide sequence that encodes a polypeptide having expansin activity, said nucleotide sequence comprising a member selected from the group consisting of:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (c) a nucleotide sequence comprising at least 70 contiguous nucleotides of a sequence of (a) or (b), wherein said sequence encodes a polypeptide having expansin activity; (d) a nucleotide sequence having at least 90% identity with the nucleotide sequences of (a), (b), or (c), wherein said sequence encodes a polypeptide having expansin activity; (e) a nucleotide sequence comprising a complementary sequence corresponding to the sequence of (a), (b), (c), or (d); and (f) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (a), (b), (c), (d), or (e) wherein said sequence encodes a polypeptide having expansin activity.
- 3. A DNA construct comprising a nucleotide sequence of claim 2, wherein said nucleotide sequence is operably linked to a promoter that drives expression in a plant cell.
- 4. An expression vector comprising the DNA construct of claim 3.
- 5. A host cell having stably incorporated into its genome at least one nucleotide sequence, wherein said nucleotide sequence is operably linked to a promoter that drives expression in the host cell, wherein said nucleotide sequence is selected from the group consisting of:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (c) a nucleotide sequence comprising at least 70 contiguous nucleotides of a sequence of (a) or (b), wherein said sequence encodes a polypeptide having expansin activity; (d) a nucleotide sequence having at least 60% identity with the nucleotide sequences of (a), (b), or (c), wherein said sequence encodes a polypeptide having expansin activity; (e) a nucleotide sequence comprising a complementary sequence corresponding to the sequence of (a), (b), (c), or (d); and (f) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (a), (b), (c), (d), or (e), wherein said sequence encodes a polypeptide having expansin activity.
- 6. The host cell of claim 5, wherein said cell is a plant cell.
- 7. A plant or parts thereof, having stably incorporated into its genome at least one nucleotide sequence, said nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (c) a nucleotide sequence comprising at least 70 contiguous nucleotides of a sequence of a), or (b), wherein said sequence encodes a polypeptide having expansin activity; (d) a nucleotide sequence having at least 60% identity with the nucleotide sequences of (a), (b), or (c), wherein said sequence encodes a polypeptide having expansin activity; (e) a nucleotide sequence comprising a complementary sequence corresponding to the sequence of (a), (b), (c), or (d); and (f) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (a), (b), (c), (d), or (e), wherein said sequence encodes a polypeptide having expansin activity.
- 8. The plant or parts thereof, of claim 7, wherein said promoter is a constitutive promoter.
- 9. The plant or parts thereof, of claim 7, wherein said promoter is a tissue-preferred promoter.
- 10. The plant or parts thereof, of claim 7, wherein said promoter is an inducible promoter.
- 11. The plant or parts thereof, of claim 7, wherein said promoter is a developmentally regulated promoter.
- 12. The plant or parts thereof, of claim 7, wherein said plant is a monocot.
- 13. The plant or parts thereof, of claim 12, wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
- 14. The plant or parts thereof, of claim 7, wherein said plant is a dicot.
- 15. A method for modulating brittle stalk resistance in a plant, said method comprising stably incorporating into the genome of said plant at least one nucleotide sequence operably linked to a promoter wherein said nucleotide sequence is selected from the group consisting of:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (c) a nucleotide sequence comprising at least 70 contiguous nucleotides of a sequence of (a) or (b), wherein said sequence encodes a polypeptide having expansin activity; (d) a nucleotide sequence having at least 60% identity with the nucleotide sequences of (a), (b), or (c), wherein said sequence encodes a polypeptide having expansin activity; (e) a nucleotide sequence comprising a complementary sequence corresponding to the sequence of (a), (b), (c), or (d); and (f) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (a), (b), (c), (d), or (e), wherein said sequence encodes a polypeptide having expansin activity.
- 16. A method for modulating expansin expression in a plant, said method comprising stably incorporating into the genome of said plant at least one nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
(a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3; (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 4; (c) a nucleotide sequence comprising at least 70 contiguous nucleotides of a sequence of (a) or (b), wherein said sequence encodes a polypeptide having expansin activity; (d) a nucleotide sequence having at least 60% identity with the nucleotide sequences of (a), (b) or (c), wherein said sequence encodes a polypeptide having expansin activity; (e) a nucleotide sequence comprising a complementary sequence corresponding to the sequence of (a), (b), (c) or (d); and (f) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (a), (b), (c), (d) or (e) wherein said sequence encodes a polypeptide having expansin activity.
- 17. The method of 16, wherein said promoter is a constituative promoter.
- 18. The method of 16, wherein said promoter is a tissue preferred promoter.
- 19. The method of 16, wherein said promoter is an inducible promoter.
- 20. The method of 16, wherein said promoter is a developmentally-regulated promoter.
- 21. The method of 16, wherein said plant is a dicot.
- 22. The method of 16, wherein said plant is a monocot.
- 23. The method of 22, wherein said monocot is maize, wheat, rice, barley, sorghum, or rye.
- 24. A method for modulating cell wall strength in a plant, said method comprising stably incorporating into the genome of said plant at least one expansin coding sequence operably linked to a Mu-tagged promoter that drives expression in a plant cell.
- 25. A method for identifying a recessive gene mutation in a F1 generation plant, said method comprising:
(a) crossing a Mu-active plant as a female parent with a B-A stock having a known deficient chromosome arm as a male parent; (b) harvesting F1 seed from said female parent of said cross; (c) planting F1 seed and allowing to grow and observing for and identifying recessive mutation phenotype in F1 plant; and (d) identifying and selecting F1 plant demonstrating mutant phenotype of interest.
- 26. A method for isolating a polynucleotide encoding a wild type gene associated with the recessive mutant identified in method of claim 24, comprising utilizing said Mu-tagged recessive gene to identify and isolate said wild-type gene.
- 27. The method of claim 25 wherein DNA from the Mu-tagged recessive mutant F1 plant is compared to wild-type siblings for Mu element polymorphisms.
- 28. The method of claim 26 wherein comparison of Mu-tagged recessive mutant plant DNA to wild-type sibling plant DNA is accomplished by Southern Hybridization or PCR techniques.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of and incorporates by reference U.S. Provisional Application Ser. Nos: 60/277,847 filed Mar. 22, 2001, and 60/324,182 filed Sep. 21, 2001.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60324182 |
Sep 2001 |
US |
|
60277847 |
Mar 2001 |
US |