Expressed sequences of arabidopsis thaliana

FIELD OF INVENTION

[0002] The invention is in the field of polynucleotide sequences of a plant, particularly sequences expressed in arabidopsis thaliana.

BACKGROUND OF THE INVENTION

[0003] Plants and plant products have vast commercial importance in a wide variety of areas including food crops for human and animal consumption, flavor enhancers for food, and production of specialty chemicals for use in products such as medicaments and fragrances. In considering food crops for humans and livestock, genes such as those involved in a plant's resistance to insects, plant viruses, and fungi; genes involved in pollination; and genes whose products enhance the nutritional value of the food, are of major importance. A number of such genes have been described, see, for example, McCaskill and Croteau (1999) Nature Biotechnol. 17:31-36.

[0004] Despite recent advances in methods for identification, cloning, and characterization of genes, much remains to be learned about plant physiology in general, including how plants produce many of the above-mentioned products; mechanisms for resistance to herbicides, insects, plant viruses, fungi; elucidation of genes involved in specific biosynthetic pathways; and genes involved in environmental tolerance, e.g., salt tolerance, drought tolerance, or tolerance to anaerobic conditions.

[0005]

Arabidopsis thaliana
is a model system for genetic, molecular and biochemical studies of higher plants. Features of this plant that make it a model system for genetic and molecular biology research include a small genome size, organized into five chromosomes and containing an estimated 20,000 genes, a rapid life cycle, prolific seed production and, since it is small, it can easily be cultivation in limited space. A. thaliana is a member of the mustard family (Brassicaceae) with a broad natural distribution throughout Europe, Asia, and North America. Many different ecotypes have been collected from natural populations and are available for experimental analysis. The entire life cycle, including seed germination, formation of a rosette plant, bolting of the main stem, flowering, and maturation of the first seeds, is completed in 6 weeks. A large number of mutant lines are available that affect nearly all aspects of its growth. These features greatly facilitate the isolation of fundamentally interesting and potentially important genes for agronomic development

[0006] Most gene products from higher plants exhibit adequate sequence similarity to deduced amino acid sequences of other plant genes to permit assignment of probable gene function, if it is known, in any higher plant. It is likely that there will be very few protein-encoding angiosperm genes that do not have orthologs or paralogs in Arabidopsis. The developmental diversity of higher plants may be largely due to changes in the cis-regulatory sequences of transcriptional regulators and not in coding sequences.

[0007] Many advances reported over the past few years offer clear evidence that this plant is not only a very important model species for basic research, but also extremely valuable for applied plant scientists and plant breeders. Knowledge gained from Arabidopsis can be used directly to develop desired traits in plants of other species.

Relevant Literature

[0008] Cold Spring Harbor Monograph 27 (1994) E. M. Meyerowitz and C. R. Somerville, eds. (CSH Laboratory Press). Annual Plant Reviews, Vol. 1: Arabidopsis (1998) M. Anderson and J. A. Roberts, eds. (CRC Press). Methods in Molecular Biology: Arabidopsis Protocols, Vol.82 (1997) J. M. Martinez-Zapater and J. Salinas, eds. (CRC Press).

[0009] Mayer et al (1999) Nature 402(6763):769-77; “Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana”. Lin et al. (1999) 402(6763):761-8, “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana”. Meinke et al. (1998) Science 282:662-682, “Arabidopsis thaliana: a model plant for genome analysis”. Somerville and Somerville (1999) Science 285:380-383, “Plant functional genomics”. Mozo et al. (1999) Nat. Genet. 22:271-275, “A complete BAC-based physical map of the Arabidopsis thaliana genome”.

SUMMARY OF THE INVENTION

[0010] Novel nucleic acid sequences of Arabidopsis thaliana, their encoded polypeptides and variants thereof, genes corresponding to these nucleic acids, and proteins expressed by the genes, are provided.

[0011] The invention also provides diagnostic, prophylactic and therapeutic agents employing such novel nucleic acids, their corresponding genes or gene products, including expression constructs, probes, antisense constructs, and the like. The genetic sequences may also be used for the genetic manipulation of plant cells, particularly dicotyledonous plants. The encoded gene products and modified organisms are useful for introducing or improving disease resistance and stress tolerance into plants; screening of biologically active agents, e.g. fungicides, etc.; for elucidating biochemical pathways; and the like.

[0012] In one embodiment of the invention, a nucleic acid is provided that comprises a start codon; an optional intervening sequence; a coding sequence capable of hybridizing under stringent conditions as set forth in SEQ ID NO:1 to 999; and an optional terminal sequence, wherein at least one of said optional sequences is present. Such a nucleic acid may correspond to naturally occurring Arabidopsis expressed sequences.

DETAILED DESCRIPTION OF THE INVENTION

[0013] Novel nucleic acid sequences from Arabidopsis thaliana, their encoded polypeptides and variants thereof, genes corresponding to these nucleic acids and proteins expressed by the genes are provided. The invention also provides agents employing such novel nucleic acids, their corresponding genes or gene products, including expression constructs, probes, antisense constructs, and the like. The nucleotide sequences are provided in the attached SEQLIST.

[0014] Sequences include, but are not limited to, sequences that encode resistance proteins; sequences that encode tolerance factors; sequences encoding proteins or other factors that are involved, directly or indirectly in biochemical pathways such as metabolic or biosynthetic pathways, sequences involved in signal transduction, sequences involved in the regulation of gene expression, structural genes, and the like. Biosynthetic pathways of interest include, but are not limited to, biosynthetic pathways whose product (which may be an end product or an intermediate) is of commercial, nutritional, or medicinal value.

[0015] The sequences may be used in screening assays of various plant strains to determine the strains that are best capable of withstanding a particular disease or environmental stress. Sequences encoding activators and resistance proteins may be introduced into plants that are deficient in these sequences. Alternatively, the sequences may be introduced under the control of promoters that are convenient for induction of expression. The protein products may be used in screening programs for insecticides, fungicides and antibiotics to determine agents that mimic or enhance the resistance proteins. Such agents may be used in improved methods of treating crops to prevent or treat disease. The protein products may also be used in screening programs to identify agents which mimic or enhance the action of tolerance factors. Such agents may be used in improved methods of treating crops to enhance their tolerance to environmental stresses.

[0016] Still other embodiments of the invention provide methods for enhancing or inhibiting production of a biosynthetic product in a plant by introducing a nucleic acid of the invention into a plant cell, where the nucleic acid comprises sequences encoding a factor which is involved, directly or indirectly in a biosynthetic pathway whose products are of commercial, nutritional, or medicinal value include any factor, usually a protein or peptide, which regulates such a biosynthetic pathway; which is an intermediate in such a biosynthetic pathway; or which in itself is a product that increases the nutritional value of a food product; or which is a medicinal product; or which is any product of commercial value.

[0017] Transgenic plants containing the antisense nucleic acids of the invention are useful for identifying other mediators that may induce expression of proteins of interest; for establishing the extent to which any specific insect and/or pathogen is responsible for damage of a particular plant; for identifying other mediators that may enhance or induce tolerance to environmental stress; for identifying factors involved in biosynthetic pathways of nutritional, commercial, or medicinal value; or for identifying products of nutritional, commercial, or medicinal value.

[0018] In still other embodiments, the invention provides transgenic plants constructed by introducing a subject nucleic acid of the invention into a plant cell, and growing the cell into a callus and then into a plant; or, alternatively by breeding a transgenic plant from the subject process with a second plant to form an F1 or higher hybrid. The subject transgenic plants and progeny are used as crops for their enhanced disease resistance, enhanced traits of interest, for example size or flavor of fruit, length of growth cycle, etc., or for screening programs, e.g. to determine more effective insecticides, etc; used as crops which exhibit enhanced tolerance environmental stress; or used to produce a factor.

[0019] Those skilled in the art will recognize the agricultural advantages inherent in plants constructed to have either increased or decreased expression of resistance proteins; or increased or decreased tolerance to environmental factors; or which produce or over-produce one or more factors involved in a biosynthetic pathway whose product is of commercial, nutritional, or medicinal value. For example, such plants may have increased resistance to attack by predators, insects, pathogens, microorganisms, herbivores, mechanical damage and the like; may be more tolerant to environmental stress, e.g. may be better able to withstand drought conditions, freezing, and the like; or may produce a product not normally made in the plant, or may produce a product in higher than normal amounts, where the product has commercial, nutritional, or medicinal value. Plants which may be useful include dicotyledons and monocotyledons. Representative examples of plants in which the provided sequences may be useful include tomato, potato, tobacco, cotton, soybean, alfalfa, rape, and the like. Monocotyledons, more particularly grasses (Poaceae family) of interest, include, without limitation, Avena sativa (oat); Avena strigosa (black oat); Elymus (wild rye); Hordeum sp. including Hordeum vulgare (barley); Oryza sp., including Oryza glaberrima (African rice); Oryza longistaminata (long-staminate rice); Pennisetum americanum (pearl millet); Sorghum sp. (sorghum); Triticum sp., including Triticum aestivum (common wheat); Triticum durum (durum wheat); Zea mays (corn); etc.

NUCLEIC ACID COMPOSITIONS

[0020] The following detailed description describes the nucleic acid compositions encompassed by the invention, methods for obtaining cDNA or genomic DNA encoding a full-length gene product, expression of these nucleic acids and genes; identification of structural motifs of the nucleic acids and genes; identification of the function of a gene product encoded by a gene corresponding to a nucleic acid of the invention; use of the provided nucleic acids as probes, in mapping, and in diagnosis; use of the corresponding polypeptides and other gene products to raise antibodies; use of the nucleic acids in genetic modification of plant and other species; and use of the nucleic acids, their encoded gene products, and modified organisms, for screening and diagnostic purposes.

[0021] The scope of the invention with respect to nucleic acid compositions includes, but is not necessarily limited to, nucleic acids having a sequence set forth in any one of SEQ ID NOS:1-999; nucleic acids that hybridize the provided sequences under stringent conditions; genes corresponding to the provided nucleic acids; variants of the provided nucleic acids and their corresponding genes, particularly those variants that retain a biological activity of the encoded gene product.

[0022] In one embodiment, the sequences of the invention provide a polypeptide coding sequence. The polypeptide coding sequence may correspond to a naturally expressed mRNA in Arabidopsis or other species, or may encode a fusion protein between one of the provided sequences and an exogenous protein coding sequence. The coding sequence is characterized by an ATG start codon, a lack of stop codons in-frame with the ATG, and a termination codon, that is, a continuous open frame is provided between the start and the stop codon. The sequence contained between the start and the stop codon will comprise a sequence capable of hybridizing under stringent conditions to a sequence set for in SEQ ID NO:1-999, and may comprise the sequence set forth in the Seqlist.

[0023] Other nucleic acid compositions contemplated by and within the scope of the present invention will be readily apparent to one of ordinary skill in the art when provided with the disclosure here.

[0024] The invention features nucleic acids that are derived from Arabidopsis thaliana. Novel nucleic acid compositions of the invention of particular interest comprise a sequence set forth in any one of SEQ ID NOS:1 -999 or an identifying sequence thereof. An “identifying sequence” is a contiguous sequence of residues at least about 10 nt to about 20 nt in length, usually at least about 50 nt to about 100 nt in length, that uniquely identifies a nucleic acid sequence, e.g., exhibits less than 90%, usually less than about 80% to about 85% sequence identity to any contiguous nucleotide sequence of more than about 20 nt. Thus, the subject novel nucleic acid compositions include full length cDNAs or mRNAs that encompass an identifying sequence of contiguous nucleotides from any one of SEQ ID NOS:1-999.

[0025] The nucleic acids of the invention also include nucleic acids having sequence similarity or sequence identity. Nucleic acids having sequence similarity are detected by hybridization under low stringency conditions, for example, at 50° C. and 10×SSC (0.9 M NaCl/0.09 M sodium citrate) and remain bound when subjected to washing at 55° C. in 1×SSC. Sequence identity can be determined by hybridization under stringent conditions, for example, at 50° C. or higher and 0.1×SSC (9 mM NaCl/0.9 mM sodium citrate). Hybridization methods and conditions are well known in the art, see U.S. Pat. No. 5,707,829. Nucleic acids that are substantially identical to the provided nucleic acid sequences, e.g. allelic variants, genetically altered versions of the gene, etc., bind to the provided nucleic acid sequences (SEQ ID NOS:1-999) under stringent hybridization conditions. By using probes, particularly labeled probes of DNA sequences, one can isolate homologous or related genes. The source of homologous genes can be any species, particularly grasses as previously described.

[0026] Preferably, hybridization is performed using at least 15 contiguous nucleotides of at least one of SEQ ID NOS:1-999. The probe will preferentially hybridize with a nucleic acid or mRNA comprising the complementary sequence, allowing the identification and retrieval of the nucleic acids of the biological material that uniquely hybridize to the selected probe. Probes of more than 15 nucleotides can be used, e.g. probes of from about 18 nucleotides up to the entire length of the provided nucleic acid sequences, but 15 nucleotides generally represents sufficient sequence for unique identification.

[0027] The nucleic acids of the invention also include naturally occurring variants of the nucleotide sequences, e.g. degenerate variants, allelic variants, etc. Variants of the nucleic acids of the invention are identified by hybridization of putative variants with nucleotide sequences disclosed herein, preferably by hybridization under stringent conditions For example, by using appropriate wash conditions, variants of the nucleic acids of the invention can be identified where the allelic variant exhibits at most about 25-30% base pair mismatches relative to the selected nucleic acid probe. In general, allelic variants contain 5-25% base pair mismatches, and can contain as little as even 2-5%, or 1-2% base pair mismatches, as well as a single base-pair mismatch.

[0028] The invention also encompasses homologs corresponding to the nucleic acids of SEQ ID NOS:1-999, where the source of homologous genes can be any related species, usually within the same genus or group. Homologs have substantial sequence similarity, e.g. at least 75% sequence identity, usually at least 90%, more usually at least 95% between nucleotide sequences. Sequence similarity is calculated based on a reference sequence, which may be a subset of a larger sequence, such as a conserved motif, coding region, flanking region, etc. A reference sequence will usually be at least about 18 contiguous nt long, more usually at least about 30 nt long, and may extend to the complete sequence that is being compared. Algorithms for sequence analysis are known in the art, such as BLAST, described in Altschul et al., J. Mol. Biol. (1990) 215:403-10.

[0029] In general, variants of the invention have a sequence identity greater than at least about 65%, preferably at least about 75%, more preferably at least about 85%, and can be greater than at least about 90% or more as determined by the Smith-Waterman homology search algorithm as implemented in MPSRCH program (Oxford Molecular). For the purposes of this invention, a preferred method of calculating percent identity is the Smith-Waterman algorithm, using the following. Global DNA sequence identity must be greater than 65% as determined by the Smith-Wateman homology search algorithm as implemented in MPSRCH program (Oxford Molecular) using an affine gap search with the following search parameters: gap open penalty, 12; and gap extention penalty, 1.

[0030] The subject nucleic acids can be cDNAs or genomic DNAs, as well as fragments thereof, particularly fragments that encode a biologically active gene product and/or are useful in the methods disclosed herein. The term “cDNA” as used herein is intended to include all nucleic acids that share the arrangement of sequence elements found in native mature mRNA species, where sequence elements are exons and 3′ and 5′ non-coding regions. Normally mRNA species have contiguous exons, with the introns, when present, being removed by nuclear RNA splicing, to create a continuous open reading frame encoding a polypeptide of the invention.

[0031] A genomic sequence of interest comprises the nucleic acid present between the initiation codon and the stop codon, as defined in the listed sequences, including all of the introns that are normally present in a native chromosome. It can further include the 3′ and 5′ untranslated regions found in the mature mRNA. It can further include specific transcriptional and translational regulatory sequences, such as promoters, enhancers, etc., including about 1 kb, but possibly more, of flanking genomic DNA at either the 5′ and 3′ end of the transcribed region. The genomic DNA can be isolated as a fragment of 100 kb or smaller; and substantially free of flanking chromosomal sequence. The genomic DNA flanking the coding region, either 3′ and 5′, or internal regulatory sequences as sometimes found in introns, contains sequences required for expression.

[0032] The nucleic acid compositions of the subject invention can encode all or a part of the subject expressed polypeptides. Double or single stranded fragments can be obtained from the DNA sequence by chemically synthesizing oligonucleotides in accordance with conventional methods, by restriction enzyme digestion, by PCR amplification, etc. Isolated nucleic acids and nucleic acid fragments of the invention comprise at least about 15 up to about 100 contiguous nucleotides, or up to the complete sequence provided in SEQ ID NOS:1-999. For the most part, fragments will be of at least 15 nt, usually at least 18 nt or 25 nt, and up to at least about 50 contiguous nt in length or more.

[0033] Probes specific to the nucleic acids of the invention can be generated using the nucleic acid sequences disclosed in SEQ ID NOS:1-999 and the fragments as described above. The probes can be synthesized chemically or can be generated from longer nucleic acids using restriction enzymes. The probes can be labeled, for example, with a radioactive, biotinylated, or fluorescent tag. Preferably, probes are designed based upon an identifying sequence of a nucleic acid of one of SEQ ID NOS:1-999. More preferably, probes are designed based on a contiguous sequence of one of the subject nucleic acids that remain unmasked following application of a masking program for masking low complexity (e.g., XBLAST) to the sequence., i.e. one would select an unmasked region, as indicated by the nucleic acids outside the poly-n stretches of the masked sequence produced by the masking program.

[0034] The nucleic acids of the subject invention are isolated and obtained in substantial purity, generally as other than an intact chromosome. Usually, the nucleic acids, either as DNA or RNA, will be obtained substantially free of other naturally-occurring nucleic acid sequences, generally being at least about 50%, usually at least about 90% pure and are typically “recombinant”, e.g., flanked by one or more nucleotides with which it is not normally associated on a naturally occurring chromosome.

[0035] The nucleic acids of the invention can be provided as a linear molecule or within a circular molecule. They can be provided within autonomously replicating molecules (vectors) or within molecules without replication sequences. They can be regulated by their own or by other regulatory sequences, as is known in the art. The nucleic acids of the invention can be introduced into suitable host cells using a variety of techniques which are available in the art, such as transferrin polycation-mediated DNA transfer, transfection with naked or encapsulated nucleic acids, liposome-mediated DNA transfer, intracellular transportation of DNA-coated latex beads, protoplast fusion, viral infection, electroporation, gene gun, calcium phosphate-mediated transfection, and the like.

[0036] The subject nucleic acid compositions can be used to, for example, produce polypeptides, as probes for the detection of mRNA of the invention in biological samples, e.g. extracts of cells, to generate additional copies of the nucleic acids, to generate ribozymes or antisense oligonucleotides, and as single stranded DNA probes or as triple-strand forming oligonucleotides. The probes described herein can be used to, for example, determine the presence or absence of the nucleic acid sequences as shown in SEQ ID NOS:1-999 or variants thereof in a sample. These and other uses are described in more detail below.

USE OF NUCLEIC ACIDS AS CODING SEQUENCES

[0037] Naturally occurring Arabidopsis polypeptides or fragments thereof are encoded by the provided nucleic acids. Methods are known in the art to determine whether the complete native protein is encoded by a candidate nucleic acid sequence. Where the provided sequence encodes a fragment of a polypeptide, methods known in the art may be used to determine the remaining sequence. These approaches may utilize a bioinformatics approach, a cloning approach, extension of mRNA species, etc.

[0038] Substantial genomic sequence is available for Arabidopsis, and may be exploited for determining the complete coding sequence corresponding to the provided sequences. The region of the chromosome to which a given sequence is located may be determined by hybridization or by database searching. The genomic sequence is then searched upstream and downstream for the presence of intron/exon boundaries, and for motifs characteristic of transcriptional start and stop sequences, for example by using Genscan (Burge and Karlin (1997) J. Mol. Biol. 268:78-94); or GRAIL (Uberbacher and Mural (1991) P.N.A.S. 88:11261-1265).

[0039] Alternatively, nucleic acid having a sequence of one of SEQ ID NOS:1-999, or an identifying fragment thereof, is used as a hybridization probe to complementary molecules in a cDNA library using probe design methods, cloning methods, and clone selection techniques as known in the art. Libraries of cDNA are made from selected cells. The cells may be those of A. thaliana, or of related species. In some cases it will be desirable to select cells from a particular stage, e.g. seeds, leaves, infected cells, etc.

[0040] Techniques for producing and probing nucleic acid sequence libraries are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., (1989) Cold Spring Harbor Press, Cold Spring Harbor, N.Y.; and Current Protocols in Molecular Biology, (1987 and updates) Ausubel et al., eds. The cDNA can be prepared by using primers based on sequence from SEQ ID NOS:1-999. In one embodiment, the cDNA library can be made from only poly-adenylated mRNA. Thus, poly-T primers can be used to prepare cDNA from the mRNA.

[0041] Members of the library that are larger than the provided nucleic acids, and preferably that encompass the complete coding sequence of the native message, are obtained. In order to confirm that the entire cDNA has been obtained, RNA protection experiments are performed as follows. Hybridization of a full-length cDNA to an mRNA will protect the RNA from RNase degradation. If the cDNA is not full length, then the portions of the mRNA that are not hybridized will be subject to RNase degradation. This is assayed, as is known in the art, by changes in electrophoretic mobility on polyacrylamide gels, or by detection of released monoribonucleotides. Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., (1989) Cold Spring Harbor Press, Cold Spring Harbor, N.Y. In order to obtain additional sequences 5′ to the end of a partial cDNA, 5′ RACE (PCR Protocols: A Guide to Methods and Applications, (1990) Academic Press, Inc.) may be performed.

[0042] Genomic DNA is isolated using the provided nucleic acids in a manner similar to the isolation of full-length cDNAs. Briefly, the provided nucleic acids, or portions thereof, are used as probes to libraries of genomic DNA. Preferably, the library is obtained from the cell type that was used to generate the nucleic acids of the invention, but this is not essential. Such libraries can be in vectors suitable for carrying large segments of a genome, such as P1 or YAC, as described in detail in Sambrook et al., 9.4-9.30. In order to obtain additional 5′ or 3′ sequences, chromosome walking is performed, as described in Sambrook et al., such that adjacent and overlapping fragments of genomic DNA are isolated. These are mapped and pieced together, as is known in the art, using restriction digestion enzymes and DNA ligase.

[0043] PCR methods may be used to amplify the members of a cDNA library that comprise the desired insert. In this case, the desired insert will contain sequence from the full length cDNA that corresponds to the instant nucleic acids. Such PCR methods include gene trapping and RACE methods. Gene trapping entails inserting a member of a cDNA library into a vector. The vector then is denatured to produce single stranded molecules. Next, a substrate-bound probe, such a biotinylated oligo, is used to trap cDNA inserts of interest. Biotinylated probes can be linked to an avidin-bound solid substrate. PCR methods can be used to amplify the trapped cDNA. To trap sequences corresponding to the full length genes, the labeled probe sequence is based on the nucleic acid sequences of the invention. Random primers or primers specific to the library vector can be used to amplify the trapped cDNA. Such gene trapping techniques are described in Gruber et al., WO 95/04745 and Gruber et al., U.S. Pat. No. 5,500,356. Kits are commercially available to perform gene trapping experiments from, for example, Life Technologies, Gaithersburg, Md., USA.

[0044] “Rapid amplification of cDNA ends”, or RACE, is a PCR method of amplifying cDNAs from a number of different RNAs. The cDNAs are ligated to an oligonucleotide linker, and amplified by PCR using two primers. One primer is based on sequence from the instant nucleic acids, for which full length sequence is desired, and a second primer comprises sequence that hybridizes to the oligonucleotide linker to amplify the cDNA. A description of this methods is reported in WO 97/19110. A common primer may be designed to anneal to an arbitrary adaptor sequence ligated to cDNA ends. When a single gene-specific RACE primer is paired with the common primer, preferential amplification of sequences between the single gene specific primer and the common primer occurs. Commercial cDNA pools modified for use in RACE are available.

[0045] Once the full-length cDNA or gene is obtained, DNA encoding variants can be prepared by site-directed mutagenesis, described in detail in Sambrook et al., 15.3-15.63. The choice of codon or nucleotide to be replaced can be based on disclosure herein on optional changes in amino acids to achieve altered protein structure and/or function. As an alternative method to obtaining DNA or RNA from a biological material, nucleic acid comprising nucleotides having the sequence of one or more nucleic acids of the invention can be synthesized.

EXPRESSION OF POLYPEPTIDES

[0046] The provided nucleic acid, e.g. a nucleic acid having a sequence of one of SEQ ID NOS:1-999), the corresponding cDNA, the polypeptide coding sequence as described above, or the full-length gene is used to express a partial or complete gene product. Constructs of nucleic acids having sequences of SEQ ID NOS:1-999 can be generated by recombinant methods, synthetically, or in a single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides is described by, e.g. Stemmer et al., Gene (Amsterdam) (1995) 164(1):49-53.

[0047] Appropriate nucleic acid constructs are purified using standard recombinant DNA techniques as described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., (1989) Cold Spring Harbor Press, Cold Spring Harbor, N.Y. The gene product encoded by a nucleic acid of the invention is expressed in any expression system, including, for example, bacterial, yeast, insect, amphibian and mammalian systems.

[0048] The subject nucleic acid molecules are generally propagated by placing the molecule in a vector. Viral and non-viral vectors are used, including plasmids. The choice of plasmid will depend on the type of cell in which propagation is desired and the purpose of propagation. Certain vectors are useful for amplifying and making large amounts of the desired DNA sequence. Other vectors are suitable for expression in cells in culture. Still other vectors are suitable for transfer and expression in cells in a whole organism or person. The choice of appropriate vector is well within the skill of the art. Many such vectors are available commercially.

[0049] The nucleic acids set forth in SEQ ID NOS:1-999 or their corresponding full-length nucleic acids are linked to regulatory sequences as appropriate to obtain the desired expression properties. These can include promoters attached either at the 5′ end of the sense strand or at the 3′ end of the antisense strand, enhancers, terminators, operators, repressors, and inducers. The promoters can be regulated or constitutive. In some situations it may be desirable to use conditionally active promoters, such as tissue-specific or developmental stage-specific promoters. These are linked to the desired nucleotide sequence using the techniques described above for linkage to vectors. Any techniques known in the art can be used.

[0050] When any of the above host cells, or other appropriate host cells or organisms, are used to replicate and/or express the nucleic acids or nucleic acids of the invention, the resulting replicated nucleic acid, RNA, expressed protein or polypeptide, is within the scope of the invention as a product of the host cell or organism. The product is recovered by any appropriate means known in the art.

IDENTIFICATION OF FUNCTIONAL AND STRUCTURAL MOTIFS

[0051] Translations of the nucleotide sequence of the provided nucleic acids, cDNAs or full genes can be aligned with individual known sequences. Similarity with individual sequences can be used to determine the activity of the polypeptides encoded by the nucleic acids of the invention. Also, sequences exhibiting similarity with more than one individual sequence can exhibit activities that are characteristic of either or both individual sequences.

[0052] The six possible reading frames may be translated using programs such as GCG pepdata, or GCG Frames (Wisconsin Package Version 10.0, Genetics Computer Group (GCG), Madison, Wis., USA. ). Programs such as ORFFinder (National Center for Biotechnology Information (NCBI) a division of the National Library of Medicine (NLM) at the National Institutes of Health (NIH) http://www.ncbi.nlm.nih.gov/) may be used to identify open reading frames (ORFs) in sequences. ORF finder identifies all possible ORFs in a DNA sequence by locating the standard and alternative stop and start codons. Other ORF identification programs include Genie (Kulp et al. (1996).

[0053] A generalized Hidden Markov Model may be used for the recognition of genes in DNA. (ISMB-96, St. Louis, Mo., AAAI/MIT Press; Reese et al. (1997), “Improved splice site detection in Genie”. Proceedings of the First Annual International Conference on Computational Molecular Biology RECOMB 1997, Santa Fe, N.M., ACM Press, New York., P. 34.); BESTORF—Prediction of potential coding fragment in human or plant EST/mRNA sequence data using Markov Chain Models; and FGENEP—Multiple genes structure prediction in plant genomic DNA (Solovyev et al. (1995) Identification of human gene structure using linear discriminant functions and dynamic programming. In Proceedings of the Third International Conference on Intelligent Systems for Molecular Biology eds. Rawling et a. Cambridge, England, AAAI Press,367-375.; Solovyev et al. (1994) Nucl. Acids Res. 22(24):5156-5163; Solovyev et al,. The prediction of human exons by oligonucleotide composition and discriminant analysis of spliceable open reading frames, in: The Second International conference on Intelligent systems for Molecular Biology (eds. Altman et al.), AAAI Press, Menlo Park, Calif. (1994, 354-362) Solovyev and Lawrence, Prediction of human gene structure using dynamic programming and oligonucleotide composition, In: Abstracts of the 4th annual Keck symposium. Pittsburgh, 47,1993; Burge and Karlin (1997) J. Mol. Biol. 268:78-94; Kulp et al. (1996) Proc. Conf. on Intelligent Systems in Molecular Biology '96, 134-142).

[0054] The full length sequences and fragments of the nucleic acid sequences of the nearest neighbors can be used as probes and primers to identify and isolate the full length sequence corresponding to provided nucleic acids. Typically, a selected nucleic acid is translated in all six frames to determine the best alignment with the individual sequences. These amino acid sequences are referred to, generally, as query sequences, which are aligned with the individual sequences. Suitable databases include Genbank, EMBL, and DNA Database of Japan (DDBJ).

[0055] Query and individual sequences can be aligned using the methods and computer programs described above, and include BLAST, available by ftp at ftp://ncbi.nim.nih.gov/.

[0056] Gapped BLAST and PSI-BLAST are useful search tools provided by NCBI. (version 2.0) (Altschul et al., 1997). Position-Specific Iterated BLAST (PSI-BLAST) provides an automated, easy-to-use version of a “profile” search, which is a sensitive way to look for sequence homologues. The program first performs a gapped BLAST database search. The PSI-BLAST program uses the information from any significant alignments returned to construct a position-specific score matrix, which replaces the query sequence for the next round of database searching. PSI-BLAST may be iterated until no new significant alignments are found. The Gapped BLAST algorithm allows gaps (deletions and insertions) to be introduced into the alignments that are returned. Allowing gaps means that similar regions are not broken into several segments. The scoring of these gapped alignments tends to reflect biological relationships more closely. The Smith-Waterman is another algorithm that produces local or global gapped sequence alignments, see Meth. Mol. Biol. (1997) 70: 173-187. Also, the GAP program using the Needleman and Wunsch global alignment method can be utilized for sequence alignments.

[0057] Results of individual and query sequence alignments can be divided into three categories, high similarity, weak similarity, and no similarity. Individual alignment results ranging from high similarity to weak similarity provide a basis for determining polypeptide activity and/or structure. Parameters for categorizing individual results include: percentage of the alignment region length where the strongest alignment is found, percent sequence identity, and e value.

[0058] The percentage of the alignment region length is calculated by counting the number of residues of the individual sequence found in the region of strongest alignment, e.g. contiguous region of the individual sequence that contains the greatest number of residues that are identical to the residues of the corresponding region of the aligned query sequence. This number is divided by the total residue length of the query sequence to calculate a percentage. For example, a query sequence of 20 amino acid residues might be aligned with a 20 amino acid region of an individual sequence. The individual sequence might be identical to amino acid residues 5, 9-15, and 17-19 of the query sequence. The region of strongest alignment is thus the region stretching from residue 9-19, an 11 amino acid stretch. The percentage of the alignment region length is: 11 (length of the region of strongest alignment) divided by (query sequence length) 20 or 55%.

[0059] Percent sequence identity is calculated by counting the number of amino acid matches between the query and individual sequence and dividing total number of matches by the number of residues of the individual sequences found in the region of strongest alignment. Thus, the percent identity in the example above would be 10 matches divided by 11 amino acids, or approximately, 90.9%

[0060] E value is the probability that the alignment was produced by chance. For a single alignment, the e value can be calculated according to Karlin et al., Proc. Natl. Acad. Sci. (1990) 87:2264 and Karlin et al., Proc. Natl. Acad. Sci. (1993) 90. The e value of multiple alignments using the same query sequence can be calculated using an heuristic approach described in Altschul et al., Nat. Genet. (1994) 6:119. Alignment programs such as BLAST program can calculate the e value.

[0061] Another factor to consider for determining identity or similarity is the location of the similarity or identity. Strong local alignment can indicate similarity even if the length of alignment is short. Sequence identity scattered throughout the length of the query sequence also can indicate a similarity between the query and profile sequences. The boundaries of the region where the sequences align can be determined according to Doolittle, supra; BLAST or FASTA programs; or by determining the area where sequence identity is highest.

[0062] In general, in alignment results considered to be of high similarity, the percent of the alignment region length is typically at least about 55% of total length query sequence; more typically, at least about 58%; even more typically; at least about 60% of the total residue length of the query sequence. Usually, percent length of the alignment region can be as much as about 62%; more usually, as much as about 64%; even more usually, as much as about 66%. Further, for high similarity, the region of alignment, typically, exhibits at least about 75% of sequence identity; more typically, at least about 78%; even more typically; at least about 80% sequence identity. Usually, percent sequence identity can be as much as about 82%; more usually, as much as about 84%; even more usually, as much as about 86%.

[0063] The p value is used in conjunction with these methods. The query sequence is considered to have a high similarity with a profile sequence when the p value is less than or equal to 10−2. Confidence in the degree of similarity between the query sequence and the profile sequence increases as the p value become smaller.

[0064] In general, where alignment results considered to be of weak similarity, there is no minimum percent length of the alignment region nor minimum length of alignment. A better showing of weak similarity is considered when the region of alignment is, typically, at least about 15 amino acid residues in length; more typically, at least about 20; even more typically; at least about 25 amino acid residues in length. Usually, length of the alignment region can be as much as about 30 amino acid residues; more usually, as much as about 40; even more usually, as much as about 60 amino acid residues. Further, for weak similarity, the region of alignment, typically, exhibits at least about 35% of sequence identity; more typically, at least about 40%; even more typically; at least about 45% sequence identity. Usually, percent sequence identity can be as much as about 50%; more usually, as much as about 55%; even more usually, as much as about 60%.

[0065] The query sequence is considered to have a low similarity with a profile sequence when the p value is greater than 10−2. Confidence in the degree of similarity between the query sequence and the profile sequence decreases as the p values become larger.

[0066] Sequence identity alone can be used to determine similarity of a query sequence to an individual sequence and can indicate the activity of the sequence. Such an alignment, preferably, permits gaps to align sequences. Typically, the query sequence is related to the profile sequence if the sequence identity over the entire query sequence is at least about 15%; more typically, at least about 20%; even more typically, at least about 25%; even more typically, at least about 50%. Sequence identity alone as a measure of similarity is most useful when the query sequence is usually, at least 80 residues in length; more usually, 90 residues; even more usually, at least 95 amino acid residues in length. More typically, similarity can be concluded based on sequence identity alone when the query sequence is preferably 100 residues in length; more preferably, 120 residues in length; even more preferably, 150 amino acid residues in length.

[0067] It is apparent, when studying protein sequence families, that some regions have been better conserved than others during evolution. These regions are generally important for the function of a protein and/or for the maintenance of its three-dimensional structure. By analyzing the constant and variable properties of such groups of similar sequences, it is possible to derive a signature for a protein family or domain, which distinguishes its members from all other unrelated proteins. A pertinent analogy is the use of fingerprints by the police for identification purposes. A fingerprint is generally sufficient to identify a given individual. Similarly, a protein signature can be used to assign a new sequence to a specific family of proteins and thus to formulate hypotheses about its function. The PROSITE database is a compendium of such fingerprints (motifs) and may be used with search software such as Wisconsin GCG Motifs to find motifs or fingerprints in query sequences. PROSITE currently contains signatures specific for about a thousand protein families or domains. Each of these signatures comes with documentation providing background information on the structure and function of these proteins (Hofmann et al. (1999) Nucleic Acids Res. 27:215-219; Bucher and Bairoch., A generalized profile syntax for biomolecular sequences motifs and its function in automatic sequence interpretation (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology; Altman et al. Eds. (1994), pp 53-61, AAAI Press, Menlo Park).

[0068] Translations of the provided nucleic acids can be aligned with amino acid profiles that define either protein families or common motifs. Also, translations of the provided nucleic acids can be aligned to multiple sequence alignments (MSA) comprising the polypeptide sequences of members of protein families or motifs. Similarity or identity with profile sequences or MSAs can be used to determine the activity of the gene products (e.g., polypeptides) encoded by the provided nucleic acids or corresponding cDNA or genes.

[0069] Profiles can designed manually by (1) creating an MSA, which is an alignment of the amino acid sequence of members that belong to the family and (2) constructing a statistical representation of the alignment. Such methods are described, for example, in Birney et al., Nucl. Acid Res. (1996) 24(14): 2730-2739. MSAs of some protein families and motifs are available for downloading to a local server. For example, the PFAM database with MSAs of 547 different families and motifs, and the software (HMMER) to search the PFAM database may be downloaded from ftp://ftp.genetics.wustl.edu/pub/eddy/pfam-4.4/ to allow secure searches on a local server. Pfam is a database of multiple alignments of protein domains or conserved protein regions., which represent evolutionary conserved structure that has implications for the protein's function (Sonnhammer et al. (1998) Nucl. Acid Res. 26:320-322; Bateman et a. (1999) Nucleic Acids Res. 27:260-262).

[0070] The 3D_ali databank (Pasarella, S. and Argos, P. (1992) Prot. Engineering 5:121-137) was constructed to incorporate new protein structural and sequence data. The databank has proved useful in many research fields such as protein sequence and structure analysis and comparison, protein folding, engineering and design and evolution. The collection enhances present protein structural knowledge by merging information from proteins of similar main-chain fold with homologous primary structures taken from large databases of all known sequences. 3D_ali databank files may be downloaded to a secure local server from http://www.embl-heidelberg.de/argos/ali/ali_form.html.

[0071] The identify and function of the gene that correlates to a nucleic acid described herein can be determined by screening the nucleic acids or their corresponding amino acid sequences against profiles of protein families. Such profiles focus on common structural motifs among proteins of each family. Publicly available profiles are known in the art.

[0072] In comparing a novel nucleic acid with known sequences, several alignment tools are available. Examples include PileUp, which creates a multiple sequence alignment, and is described in Feng et al., J. Mol. Evol. (1987) 25:351. Another method, GAP, uses the alignment method of Needleman et al., J. Mol. Biol. (1970) 48:443. GAP is best suited for global alignment of sequences. A third method, BestFit, functions by inserting gaps to maximize the number of matches using the local homology algorithm of Smith et a. (1981) Adv. Appl. Math. 2:482.

IDENTIFICATION OF SECRETED & MEMBRANE-BOUND POLYPEPTIDES

[0073] Secreted and membrane-bound polypeptides of the present invention are of interest. Because both secreted and membrane-bound polypeptides comprise a fragment of contiguous hydrophobic amino acids, hydrophobicity predicting algorithms can be used to identify such polypeptides. A signal sequence is usually encoded by both secreted and membrane-bound polypeptide genes to direct a polypeptide to the surface of the cell. The signal sequence usually comprises a stretch of hydrophobic residues. Such signal sequences can fold into helical structures. Membrane-bound polypeptides typically comprise at least one transmembrane region that possesses a stretch of hydrophobic amino acids that can transverse the membrane. Some transmembrane regions also exhibit a helical structure. Hydrophobic fragments within a polypeptide can be identified by using computer algorithms. Such algorithms include Hopp & Woods, Proc. Natl. Acad. Sci. USA (1981) 78:3824-3828; Kyte & Doolittle, J. Mol. Biol. (1982) 157: 105-132; and RAOAR algorithm, Degli Esposti et al., Eur. J. Biochem. (1990) 190: 207-219.

[0074] Another method of identifying secreted and membrane-bound polypeptides is to translate the nucleic acids of the invention in all six frames and determine if at least 8 contiguous hydrophobic amino acids are present. Those translated polypeptides with at least 8; more typically, 10; even more typically, 12 contiguous hydrophobic amino acids are considered to be either a putative secreted or membrane bound polypeptide. Hydrophobic amino acids include alanine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, tyrosine, and valine.

IDENTIFICATION OF THE FUNCTION OF AN EXPRESSION PRODUCT

[0075] The biological function of the encoded gene product of the invention may be determined by empirical or deductive methods. One promising avenue, termed phylogenomics, exploits the use of evolutionary information to facilitate assignment of gene function. The approach is based on the idea that functional predictions can be greatly improved by focusing on how genes became similar in sequence during evolution instead of focusing on the sequence similarity itself. One of the major efficiencies that has emerged from plant genome research to date is that a large percentage of higher plant genes can be assigned some degree of function by comparing them with the sequences of genes of known function.

[0076] Alternatively, “reverse genetics” is used to identify gene function. Large collections of insertion mutants are available for Arabidopsis, maize, petunia, and snapdragon. These collections can be screened for an insertional inactivation of any gene by using the polymerase chain reaction (PCR) primed with oligonucleotides based on the sequences of the target gene and the insertional mutagen. The presence of an insertion in the target gene is indicated by the presence of a PCR product. By multiplexing DNA samples, hundreds of thousands of lines can be screened and the corresponding mutant plants can be identified with relatively small effort. Analysis of the phenotype and other properties of the corresponding mutant will provide an insight into the function of the gene.

[0077] In one method of the invention, the gene function in a transgenic Arabidopsis plant is assessed with anti-sense constructs. A high degree of gene duplication is apparent in Arabidopsis, andmany of the gene duplications in Arabidopsis are very tightly linked. Large numbers of transgenic Arabidopsis plants can be generated by infecting flowers with Agrobacterium tumefaciens containing an insertional mutagen, a method of gene silencing based on producing double-stranded RNA from bidirectional transcription of genes in transgenic plants can be broadly useful for high-throughput gene inactivation (Clough and Bent (1999) Plant J. 17; Waterhouse et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:13959). This method may use promoters that are expressed in only a few cell types or at a particular developmental stage or in response to an external stimulus. This could significantly obviate problems associated with the lethality of some mutations.

[0078] Virus-induced gene silencing may also find use for suppressing gene function. This method exploits the fact that some or all plants have a surveillance system that can specifically recognize viral nucleic acids and mount a sequence-specific suppression of viral RNA accumulation. By inoculating plants with a recombinant virus containing part of a plant gene, it is possible to rapidly silence the endogenous plant gene.

[0079] Antisense nucleic acids are designed to specifically bind to RNA, resulting in the formation of RNA-DNA or RNA-RNA hybrids, with an arrest of DNA replication, reverse transcription or messenger RNA translation. Antisense nucleic acids based on a selected nucleic acid sequence can interfere with expression of the corresponding gene. Antisense nucleic acids are typically generated within the cell by expression from antisense constructs that contain the antisense strand as the transcribed strand. Antisense nucleic acids based on the disclosed nucleic acids will bind and/or interfere with the translation of mRNA comprising a sequence complementary to the antisense nucleic acid. The expression products of control cells and cells treated with the antisense construct are compared to detect the protein product of the gene corresponding to the nucleic acid upon which the antisense construct is based. The protein is isolated and identified using routine biochemical methods.

[0080] As an alternative method for identifying function of the gene corresponding to a nucleic acid disclosed herein, dominant negative mutations are readily generated for corresponding proteins that are active as homomultimers. A mutant polypeptide will interact with wild-type polypeptides (made from the other allele) and form a non-functional multimer. Thus, a mutation is in a substrate-binding domain, a catalytic domain, or a cellular localization domain. Preferably, the mutant polypeptide will be overproduced. Point mutations are made that have such an effect. In addition, fusion of different polypeptides of various lengths to the terminus of a protein can yield dominant negative mutants. General strategies are available for making dominant negative mutants (see for example, Herskowitz (1987) Nature 329:219). Such techniques can be used to create loss of function mutations, which are useful for determining protein function.

[0081] Another approach for discovering the function of genes utilizes gene chips and microarrays. DNA sequences representing all the genes in an organism can be placed on miniature solid supports and used as hybridization substrates to quantitate the expression of all the genes represented in a complex mRNA sample. This information is used to provide extensive databases of quantitative information about the degree to which each gene responds to pathogens, pests, drought, cold, salt, photoperiod, and other environmental variation. Similarly, one obtains extensive information about which genes respond to changes in developmental processes such as germination and flowering. One can therefore determine which genes respond to the phytohormones, growth regulators, safeners, herbicides, and related agrichemicals. These databases of gene expression information provide insights into the “pathways” of genes that control complex responses. The accumulation of DNA microarray or gene chip data from many different experiments creates a powerful opportunity to assign functional information to genes of otherwise unknown function. The conceptual basis of the approach is that genes that contribute to the same biological process will exhibit similar patterns of expression. Thus, by clustering genes based on the similarity of their relative levels of expression in response to diverse stimuli or developmental or environmental conditions, it is possible to assign functions to many genes based on the known function of other genes in the cluster.

CONSTRUCTION OF POLYPEPTIDES OF THE INVENTION AND VARIANTS THEREOF

[0082] The polypeptides of the invention include those encoded by the disclosed nucleic acids. These polypeptides can also be encoded by nucleic acids that, by virtue of the degeneracy of the genetic code, are not identical in sequence to the disclosed nucleic acids. Thus, the invention includes within its scope a polypeptide encoded by a nucleic acid having the sequence of any one of SEQ ID NOS: 1-999 or a variant thereof.

[0083] In general, the term “polypeptide” as used herein refers to both the full length polypeptide encoded by the recited nucleic acid, the polypeptide encoded by the gene represented by the recited nucleic acid, as well as portions or fragments thereof. “Polypeptides” also includes variants of the naturally occurring proteins, where such variants are homologous or substantially similar to the naturally occurring protein, and can be of an origin of the same or different species as the naturally occurring protein. In general, variant polypeptides have a sequence that has at least about 80%, usually at least about 90%, and more usually at least about 98% sequence identity with a differentially expressed polypeptide of the invention, as measured by BLAST using the parameters described above. The variant polypeptides can be naturally or non-naturally glycosylated, i.e., the polypeptide has a glycosylation pattern that differs from the glycosylation pattern found in the corresponding naturally occurring protein.

[0084] In general, the polypeptides of the subject invention are provided in a non-naturally occurring environment, e.g. are separated from their naturally occurring environment. In certain embodiments, the subject protein is present in a composition that is enriched for the protein as compared to a control. As such, purified polypeptide is provided, where by purified is meant that the protein is present in a composition that is substantially free of non-differentially expressed polypeptides, where by substantially free is meant that less than 90%, usually less than 60% and more usually less than 50% of the composition is made up of non-differentially expressed polypeptides.

[0085] Also within the scope of the invention are variants; variants of polypeptides include mutants, fragments, and fusions. Mutants can include amino acid substitutions, additions or deletions. The amino acid substitutions can be conservative amino acid substitutions or substitutions to eliminate non-essential amino acids, such as to alter a glycosylation site, a phosphorylation site or an acetylation site, or to minimize misfolding by substitution or deletion of one or more cysteine residues that are not necessary for function. Conservative amino acid substitutions are those that preserve the general charge, hydrophobicity/hydrophilicity, and/or steric bulk of the amino acid substituted.

[0086] Variants also include fragments of the polypeptides disclosed herein, particularly biologically active fragments and/or fragments corresponding to functional domains. Fragments of interest will typically be at least about 10 amino acids (aa) to at least about 15 aa in length, usually at least about 50 aa in length, and can be as long as 300 aa in length or longer, but will usually not exceed about 1000 aa in length, where the fragment will have a stretch of amino acids that is identical to a polypeptide encoded by a nucleic acid having a sequence of any SEQ ID NOS:1-999, or a homolog thereof.

[0087] The protein variants described herein are encoded by nucleic acids that are within the scope of the invention. The genetic code can be used to select the appropriate codons to construct the corresponding variants.

LIBRARIES AND ARRAYS

[0088] In general, a library of biopolymers is a collection of sequence information, which information is provided in either biochemical form (e.g., as a collection of nucleic acid or polypeptide molecules), or in electronic form (e.g., as a collection of genetic sequences stored in a computer-readable form, as in a computer system and/or as part of a computer program). The term biopolymer, as used herein, is intended to refer to polypeptides, nucleic acids, and derivatives thereof, which molecules are characterized by the possession of genetic sequences either corresponding to, or encoded by, the sequences set forth in the provided sequence list (seqlist). The sequence information can be used in a variety of ways, e.g., as a resource for gene discovery, as a representation of sequences expressed in a selected cell type, e.g. cell type markers, etc.

[0089] The nucleic acid libraries of the subject invention include sequence information of a plurality of nucleic acid sequences, where at least one of the nucleic acids has a sequence of any of SEQ ID NOS:1-999. By plurality is meant one or more, usually at least 2 and can include up to all of SEQ ID NOS:1-999. The length and number of nucleic acids in the library will vary with the nature of the library, e.g., if the library is an oligonucleotide array, a cDNA array, a computer database of the sequence information, etc.

[0090] Where the library is an electronic library, the nucleic acid sequence information can be present in a variety of media. “Media” refers to a manufacture, other than an isolated nucleic acid molecule, that contains the sequence information of the present invention. Such a manufacture provides the sequences or a subset thereof in a form that can be examined by means not directly applicable to the sequence as it exists in a nucleic acid. For example, the nucleotide sequence of the present invention, e.g. the nucleic acid sequences of any of the nucleic acids of SEQ ID NOS:1-999, can be recorded on computer readable media, e.g. any medium that can be read and accessed directly by a computer. Such media include, but are not limited to: magnetic storage media, such as a floppy disc, a hard disc storage medium, and a magnetic tape; optical storage media such as CD-ROM; electrical storage media such as RAM and ROM; and hybrids of these categories such as magnetic/optical storage media. One of skill in the art can readily appreciate how any of the presently known computer readable mediums can be used to create a manufacture comprising a recording of the present sequence information. “Recorded” refers to a process for storing information on computer readable medium, using any such methods as known in the art. Any convenient data storage structure can be chosen, based on the means used to access the stored information. A variety of data processor programs and formats can be used for storage, e.g. word processing text file, database format, etc. In addition to the sequence information, electronic versions of the libraries of the invention can be provided in conjunction or connection with other computer-readable information and/or other types of computer-readable files (e.g., searchable files, executable files, etc, including, but not limited to, for example, search program software, etc.)

[0091] By providing the nucleotide sequence in computer readable form, the information can be accessed for a variety of purposes. Computer software to access sequence information is publicly available. For example, the BLAST (Altschul et al., supra.) and BLAZE (Brutlag et al. Comp. Chem. (1993) 17:203) search algorithms on a Sybase system can be used identify open reading frames (ORFs) within the genome that contain homology to ORFs from other organisms.

[0092] As used herein, “a computer-based system” refers to the hardware means, software means, and data storage means used to analyze the nucleotide sequence information of the present invention. The minimum hardware of the computer-based systems of the present invention comprises a central processing unit (CPU), input means, output means, and data storage means. A skilled artisan can readily appreciate that any one of the currently available computer-based system are suitable for use in the present invention. The data storage means can comprise any manufacture comprising a recording of the present sequence information as described above, or a memory access means that can access such a manufacture.

[0093] “Search means” refers to one or more programs implemented on the computer-based system, to compare a target sequence or target structural motif with the stored sequence information. Search means are used to identify fragments or regions of the genome that match a particular target sequence or target motif. A variety of known algorithms are publicly known and commercially available, e.g. MacPattern (EMBL), BLASTN, BLASTX (NCBI) and tBLASTX. A “target sequence” can be any DNA or amino acid sequence of six or more nucleotides or two or more amino acids, preferably from about 10 to 100 amino acids or from about 30 to 300 nucleotide residues.

[0094] A “target structural motif,” or “target motif,” refers to any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration that is formed upon the folding of the target motif, or on consensus sequences of regulatory or active sites. There are a variety of target motifs known in the art. Protein target motifs include, but arc not limited to, enzyme active sites and signal sequences. Nucleic acid target motifs include, but are not limited to, hairpin structures, promoter sequences and other expression elements such as binding sites for transcription factors.

[0095] A variety of structural formats for the input and output means can be used to input and output the information in the computer-based systems of the present invention. One format for an output means ranks fragments of the genome possessing varying degrees of homology to a target sequence or target motif. Such presentation provides a skilled artisan with a ranking of sequences and identifies the degree of sequence similarity contained in the identified fragment.

[0096] A variety of comparing means can be used to compare a target sequence or target motif with the data storage means to identify sequence fragments of the genome. A skilled artisan can readily recognize that any one of the publicly available homology search programs can be used as the search means for the computer based systems of the present invention.

[0097] As discussed above, the “library” of the invention also encompasses biochemical libraries of the nucleic acids of SEQ ID NOS:1-999, e.g., collections of nucleic acids representing the provided nucleic acids. The biochemical libraries can take a variety of forms, e.g. a solution of cDNAs, a pattern of probe nucleic acids stably bound to a surface of a solid support (microarray) and the like. By array is meant an article of manufacture that has a solid support or substrate with one or more nucleic acid targets on one of its surfaces, where the number of distinct nucleic may be in the hundreds, thousand, or tens of thousands. Each nucleic acid will comprise at 18 nt and often at least 25 nt, and often at least 100 to 1000 nucleotides, and may represent up to a complete coding sequence or cDNA. A variety of different array formats have been developed and are known to those of skill in the art. The arrays of the subject invention find use in a variety of applications, including gene expression analysis, drug screening, mutation analysis and the like, as disclosed in the above-listed exemplary patent documents.

[0098] In addition to the above nucleic acid libraries, analogous libraries of polypeptides are also provided, where the where the polypeptides of the library will represent at least a portion of the polypeptides encoded by SEQ ID NOS:1-999.

GENETICALLY ALTERED CELLS AND TRANSGENICS

[0099] The subject nucleic acids can be used to create genetically modified and transgenic organisms, usually plant cells and plants, which may be monocots or dicots. The term transgenic, as used herein, is defined as an organism into which an exogenous nucleic acid construct has been introduced, generally the exogenous sequences are stably maintained in the genome of the organism. Of particular interest are transgenic organisms where the genomic sequence of germ line cells has been stably altered by introduction of an exogenous construct.

[0100] Typically, the transgenic organism is altered in the genetic expression of the introduced nucleotide sequences as compared to the wild-type, or unaltered organism. For example, constructs that provide for over-expression of a targeted sequence, sometimes referred to as a “knock-in”, provide for increased levels of the gene product. Alternatively, expression of the targeted sequence can be down-regulated or substantially eliminated by introduction of a “knock-out” construct, which may direct transcription of an anti-sense RNA that blocks expression of the naturally occurring mRNA, by deletion of the genomic copy of the targeted sequence, etc.

[0101] In one method, large numbers of genes are simultaneously introduced in order to explore the genetic basis of complex traits, for example by making plant artificial chromosome (PLAC) libraries. The centromeres in Arabidopsis have been mapped and current genome sequencing efforts will extend through these regions. Because Arabidopsis telomeres are very similar to those in yeast one may use a hybrid sequence of alternating plant and yeast sequences that function in both types of organisms, developing yeast artificial chromosome-PLAC libraries, and then introducing them into a suitable plant host to evaluate the phenotypic consequences. By providing a defined chromosomal environment for cloned genes, the use of PLACs may also enhance the ability to produce transgenic plants with defined levels of gene expression.

[0102] It has been found in many organisms that there is significant redundancy in the representation of genes in a genome. That is, a particular gene function is likely by represented by multiple copies of similar coding sequences in the genome. These copies are typically conserved in the amino acid sequence, but may diverge in the sequence of non-translated sequences, and in their codon usage. In order to knock out a particular genetic function in an organism, it may not be sufficient to delete a genomic copy of a single gene. In such cases it may be preferable to achieve a genetic knock-out with an anti-sense construct, particularly where the sequence is aligned with the coding portion of the mRNA.

[0103] Methods of transforming plant cells are well-known in the art, and include protoplast transformation, tungsten whiskers (Coffee et al., U.S. Pat. No. 5,302,523, issued Apr. 12, 1994), directly by microorganisms with infectious plasmids, use of transposons (U.S. Pat. No. 5,792,294), infectious viruses, the use of liposomes, microinjection by mechanical or laser beam methods, by whole chromosomes or chromosome fragments, electroporation, silicon carbide fibers, and microprojectile bombardment.

[0104] For example, one may utilize the biolistic bombardment of meristem tissue, at a very early stage of development, and the selective enhancement of transgenic sectors toward genetic homogeneity, in cell layers that contribute to germline transmission. Biolistics-mediated production of fertile, transgenic maize is described in Gordon-Kamm et al. (1990), Plant Cell 2:603; Fromm et al. (1990) Bio/Technology 8: 833, for example. Alternatively, one may use a microorganism, including but not limited to, Agrobacterium tumefaciens as a vector for transforming the cells, particularly where the targeted plant is a dicotyledonous species. See, for example, U.S. Pat. No. 5,635,381. Leung et al. (1990) Curr. Genet. 17(5):409-11 describe integrative transformation of three fertile hermaphroditic strains of Arabidopsis thaliana using plasmids and cosmids that contain an E. coli gene linked to Aspergillus nidulans regulatory sequences.

[0105] Preferred expression cassettes for cereals may include promoters that are known to express exogenous DNAs in corn cells. For example, the Adhl promoter has been shown to be strongly expressed in callus tissue, root tips, and developing kernels in corn. Promoters that are used to express genes in corn include, but are not limited to, a plant promoter such as the, CaMV 35S promoter (Odell et al., Nature, 313, 810 (1985)), or others such as CaMV 19S (Lawton et al., Plant Mol. Biol., 9, 31F (1987)), nos (Ebert et al., PNAS USA, 84, 5745 (1987)), Adh (Walker et al., PNAS USA, 84, 6624 (1987)), sucrose synthase (Yang et al., PNAS USA, 87, 4144 (1990)), .alpha.-tubulin, ubiquitin, actin (Wang et al., Mol. Cell. Biol., 12, 3399 (1992)), cab (Sullivan et al., Mol. Gen. Genet, 215, 431 (1989)), PEPCase (Hudspeth et al., Plant Mol. Biol., 12, 579 (1989)), or those associated with the R gene complex (Chandler et al., The Plant Cell, 1, 1175 (1989)). Other promoters useful in the practice of the invention are known to those of skill in the art.

[0106] Tissue-specific promoters, including but not limited to, root-cell promoters (Conkling et al., Plant Physiol., 93, 1203 (1990)), and tissue-specific enhancers (Fromm et al., The Plant Cell, 1, 977 (1989)) are also contemplated to be particularly useful, as are inducible promoters such as water-stress-, ABA- and turgor-inducible promoters (Guerrero et al., Plant Molecular Biology, 15, 11-26)), and the like.

[0107] Regulating and/or limiting the expression in specific tissues may be functionally accomplished by introducing a constitutively expressed gene (all tissues) in combination with an antisense gene that is expressed only in those tissues where the gene product is not desired. Expression of an antisense transcript of this preselected DNA segment in an rice grain, using, for example, a zein promoter, would prevent accumulation of the gene product in seed. Hence the protein encoded by the preselected DNA would be present in all tissues except the kernel.

[0108] Alternatively, one may wish to obtain novel tissue-specific promoter sequences for use in accordance with the present invention. To achieve this, one may first isolate cDNA clones from the tissue concerned and identify those clones which are expressed specifically in that tissue, for example, using Northern blotting or DNA microarrays. Ideally, one would like to identify a gene that is not present in a high copy number, but which gene product is relatively abundant in specific tissues. The promoter and control elements of corresponding genomic clones may then be localized using the techniques of molecular biology known to those of skill in the art. Alternatively, promoter elements can be identified using enhancer traps based on T-DNA and/or transposon vector systems (see, for example, Campisi et al. (1999) Plant J. 17:699-707; Gu et al. (1998) Development 125:1509-1517).

[0109] In some embodiments of the present invention expression of a DNA segment in a transgenic plant will occur only in a certain time period during the development of the plant. Developmental timing is frequently correlated with tissue specific gene expression. For example, in corn expression of zein storage proteins is initiated in the endosperm about 15 days after pollination.

[0110] Ultimately, the most desirable DNA segments for introduction into a plant genome may be homologous genes or gene families which encode a desired trait (e.g., increased disease resistance) and which are introduced under the control of novel promoters or enhancers, etc., or perhaps even homologous or tissue-specific (e.g., root-,grain- or leaf-specific) promoters or control elements.

[0111] The genetically modified cells are screened for the presence of the introduced genetic material. The cells may be used in functional studies, drug screening, etc., e.g. to study chemical mode of action, to determine the effect of a candidate agent on pathogen growth, infection of plant cells, etc.

[0112] The modified cells are useful in the study of genetic function and regulation, for alteration of the cellular metabolism, and for screening compounds that may affect the biological function of the gene or gene product. For example, a series of small deletions and/or substitutions may be made in the host's native gene to determine the role of different domains and motifs in the biological function. Specific constructs of interest include anti-sense, as previously described, which will reduce or abolish expression, expression of dominant negative mutations, and over-expression of genes.

[0113] Where a sequence is introduced, the introduced sequence may be either a complete or partial sequence of a gene native to the host, or may be a complete or partial sequence that is exogenous to the host organism, e.g., an A. thaliana sequence inserted into wheat plants. A detectable marker, such as aldA, lac Z, etc. may be introduced into the locus of interest, where upregulation of expression will result in an easily detected change in phenotype.

[0114] One may also provide for expression of the gene or variants thereof in cells or tissues where it is not normally expressed, at levels not normally present in such cells or tissues, or at abnormal times of development, during sporulation, etc. By providing expression of the protein in cells in which it is not normally produced, one can induce changes in cell behavior.

[0115] DNA constructs for homologous recombination will comprise at least a portion of the provided gene or of a gene native to the species of the host organism, wherein the gene has the desired genetic modification(s), and includes regions of homology to the target locus (see Kempin et al. (1997) Nature 389:802-803). DNA constructs for random integration or episomal maintenance need not include regions of homology to mediate recombination. Conveniently, markers for positive and negative selection are included. Methods for generating cells having targeted gene modifications through homologous recombination are known in the art.

[0116] Embodiments of the invention provide processes for enhancing or inhibiting synthesis of a protein in a plant by introducing a provided nucleic acids sequence into a plant cell, where the nucleic acid comprises sequences encoding a protein of interest. For example, enhanced resistance to pathogens may be achieved by inserting a nucleic acid encoding an activator in a vector downstream from a promoter sequence capable of driving constitutive high-level expression in a plant cell. When grown into plants, the transgenic plants exhibit increased synthesis of resistance proteins, and increased resistance to pathogens.

[0117] Other embodiments of the invention provide processes for enhancing or inhibiting synthesis of a tolerance factor in a plant by introducing a nucleic acid of the invention into a plant cell, where the nucleic acid comprises sequences encoding a tolerance factor. For example, enhanced tolerance to an environmental stress may be achieved by inserting a nucleic acid encoding an activator in a vector downstream from a promoter sequence capable of driving constitutive high-level expression in a plant cell. When grown into plants, the transgenic plants exhibit increased synthesis of tolerance proteins, and increased tolerance to environmental stress.

[0118] Factors which are involved, directly or indirectly in biosynthetic pathways whose products are of commercial, nutritional, or medicinal value include any factor, usually a protein or peptide, which regulates such a biosynthetic pathway (e.g., an activator or repressor); which is an intermediate in such a biosynthetic pathway; or which is a product that increases the nutritional value of a food product; a medicinal product; or any product of commercial value and/or research interest. Plant and other cells may be genetically modified to enhance a trait of interest, by upregulating or down-regulating factors in a biosynthetic pathway.

SCREENING ASSAYS

[0119] The polypeptides encoded by the provided nucleic acid sequences, and cells genetically altered to express such sequences, are useful in a variety of screening assays to determine effect of candidate inhibitors, activators., or modifiers of the gene product. One may determine what insecticides, fungicides and the like have an enhancing or synergistic activity with a gene. Alternatively, one may screen for compounds that mimic the activity of the protein. Similarly, the effect of activating agents may be used to screen for compounds that mimic or enhance the activation of proteins. Candidate inhibitors of a particular gene product are screened by detecting decreased from the targeted gene product.

[0120] The screening assays may use purified target macromolecules to screen large compound libraries for inhibitory drugs; or the purified target molecule may be used for a rational drug design program, which requires first determining the structure of the macromolecular target or the structure of the macromolecular target in association with its customary substrate or ligand. This information is then used to design compounds which must be synthesized and tested further. Test results are used to refine the molecular models and drug design process in an iterative fashion until a lead compound emerges.

[0121] Drug screening may be performed using an in vitro model, a genetically altered cell, or purified protein. One can identify ligands or substrates that bind to, modulate or mimic the action of the target genetic sequence or its product. A wide variety of assays may be used for this purpose, including labeled in vitro protein-protein binding assays, electrophoretic mobility shift assays, immunoassays for protein binding, and the like. The purified protein may also be used for determination of three-dimensional crystal structure, which can be used for modeling intermolecular interactions.

[0122] Where the nucleic acid encodes a factor involved in a biosynthetic pathway, as described above, it may be desirable to identify factors, e.g., protein factors, which interact with such factors. One can identify interacting factors, ligands, substrates that bind to, modulate or mimic the action of the target genetic sequence or its product. A wide variety of assays may be used for this purpose, including labeled in vitro protein-protein binding assays, electrophoretic mobility shift assays, immunoassays for protein binding, and the like. In vivo assays for protein-protein interactions in E. coli and yeast cells are also well-established (see Hu et a. (2000) Methods 20:80-94; and Bai and Elledge (1997) Methods Enzymol. 283:141-156).

[0123] The purified protein may also be used for determination of three-dimensional crystal structure, which can be used for modeling intermolecular interactions. It may also be of interest to identify agents that modulate the interaction of a factor identified as described above with a factor encoded by a nucleic acid of the invention. Drug screening can be performed to identify such agents. For example, a labeled in vitro protein-protein binding assay can be used, which is conducted in the presence and absence of an agent being tested.

[0124] The term “agent” as used herein describes any molecule, e.g. protein or pharmaceutical, with the capability of altering or mimicking a physiological function. Generally a plurality of assay mixtures are run in parallel with different agent concentrations to obtain a differential response to the various concentrations. Typically, one of these concentrations serves as a negative control, i.e. at zero concentration or below the level of detection.

[0125] Candidate agents encompass numerous chemical classes, though typically they are organic molecules, preferably small organic compounds having a molecular weight of more than 50 and less than about 2,500 daltons. Candidate agents comprise functional groups necessary for structural interaction with proteins, particularly hydrogen bonding, and typically include at least an amine, carbonyl, hydroxyl or carboxyl group, preferably at least two of the functional chemical groups. The candidate agents often comprise cyclical carbon or heterocyclic structures and/or aromatic or polyaromatic structures substituted with one or more of the above functional groups. Candidate agents are also found among biomolecules including peptides, saccharides, fatty acids, steroids, purines, pyrimidines, derivatives, structural analogs or combinations thereof.

[0126] Candidate agents are obtained from a wide variety of sources including libraries of synthetic or natural compounds. For example, numerous means are available for random and directed synthesis of a wide variety of organic compounds and biomolecules, including expression of randomized oligonucleotides and oligopeptides. Alternatively, libraries of natural compounds in the form of bacterial, fungal, plant and organism extracts are available or readily produced. Additionally, natural or synthetically produced libraries and compounds are readily modified through conventional chemical, physical and biochemical means, and may be used to produce combinatorial libraries. Known pharmacological agents may be subjected to directed or random chemical modifications, such as acylation, alkylation, esterification, amidification, etc. to produce structural analogs.

[0127] Where the screening assay is a binding assay, one or more of the molecules may be joined to a label, where the label can directly or indirectly provide a detectable signal. Various labels include radioisotopes, fluorescers, chemiluminescers, enzymes, specific binding molecules, particles, e.g. magnetic particles, and the like. Specific binding molecules include pairs, such as biotin and streptavidin, digoxin and antidigoxin etc. For the specific binding members, the complementary member would normally be labeled with a molecule that provides for detection, in accordance with known procedures.

[0128] A variety of other reagents may be included in the screening assay. These include reagents like salts, neutral proteins, e.g. albumin, detergents, etc that are used to facilitate optimal protein-protein binding and/or reduce non-specific or background interactions. Reagents that improve the efficiency of the assay, such as protease inhibitors, nuclease inhibitors, anti-microbial agents, etc. may be used. The mixture of components are added in any order that provides for the requisite binding. Incubations are performed at any suitable temperature, typically between 4 and 40° C. Incubation periods are selected for optimum activity, but may also be optimized to facilitate rapid high-throughput screening. Typically between 0.1 and 1 hours will be sufficient.

[0129] The compounds having the desired biological activity may be administered in an acceptable carrier to a host. The active agents may be administered in a variety of ways. Depending upon the manner of introduction, the compounds may be formulated in a variety of ways. The concentration of therapeutically active compound in the formulation may vary from about 0.01-100 wt. %.

[0130] It must be noted that as used herein and in the appended claims, the singular forms “a”, “and”, and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a complex” includes a plurality of such complexes and reference to “the formulation” includes reference to one or more formulations and equivalents thereof known to those skilled in the art, and so forth.

[0131] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this invention belongs. Although any methods, devices and materials similar or equivalent to those described herein can be used in the practice or testing of the invention, the preferred methods, devices and materials are now described.

[0132] All publications mentioned herein are incorporated herein by reference for the purpose of describing and disclosing, for example, the methods and methodologies that are described in the publications which might be used in connection with the presently described invention. The publications discussed above and throughout the text are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior invention.

[0133] The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use the subject invention, and are not intended to limit the scope of what is regarded as the invention. Efforts have been made to ensure accuracy with respect to the numbers used (e.g. amounts, temperature, concentrations, etc.) but some experimental errors and deviations should be allowed for. Unless otherwise indicated, parts are parts by weight, molecular weight is average molecular weight, temperature is in degrees Celsius, and pressure is at or near atmospheric.

EXPERIMENTAL

Cloning and Characterization of Arabidopsis thaliana Genes.

[0134] Following DNA isolation, sequencing was performed using the Dye Primer Sequencing protocol, below. The sequencing reactions were loaded by hand onto a 48 lane ABI 377 and run on a 36 cm gel with the 36E-2400 run module and extraction. Gel analysis was performed with ABI software.

[0135] The Phred program was used to read the sequence trace from the ABI sequencer, call the bases and produce a sequence read and a quality score for each base call in the sequence., (Ewing et al. (1998) Genome Research 8:175-185; Ewing and Green (1998) Genome Research 8:186-194.) PolyPhred may be used to detect single nucleotide polymorphisms in sequences (Kwok et al. (1994) Genomics 25:615-622; Nickerson et al. (1997) Nucleic Acids Research 25(14):2745-2751.)

[0136] MicroWave Plasmid Protocol: Fill Beckman 96 deep-well growth blocks with 1 ml of TB containing 50 μg of ampicillin per ml. Inoculate each well with a colony picked with a toothpick or a 96-pin tool from a glycerol stock plate. Cover the blocks with a plastic lid and tape at two ends to hold lid in place. Incubate overnight (16-24 hours depending on the host stain) at 37° C. with shaking at 275 rpm in a New Brunswick platform shaker. Pellet cells by centrifugation for 20 minutes at 3250 rpm in a Beckman GS-R6K, decant TB and freeze pelleted cell in the 96 well block. Thaw blocks on the bench when ready to continue.

1Prepare the MW-Tween20 solutionFor four blocks:For 16 blocks:50 ml STET/TWEEN20200 ml STET/TWEEN2 tubes RNAse (10 mg/ml,600 ulea)8 tubes RNAse1 tube lysozyme (25 mg)4 tubes lysozyme

[0137] Pipette RNAse and Lysozyme into the corner of a beaker. Add Tween 20 solution and swirl to mix completely. Use the Multidrop (or Biohit) to add 25 ul of sterile H2O (from the L size autoclaved bottles) to each well. Resuspend the pellets by vortexing on setting 10 of the platform vortexer. Check pellets after 4 min. and repeat as necessary to resuspend completely. Use the multidrop to add 70 μl of the freshly prepared MW-Tween 20 solution to each well. Vortex at setting 6 on the platform vortex for 15 seconds. Do not cause frothing.

[0138] Incubate the blocks at room temperature for 5 min. Place two blocks at a time in the microwave (1000 Watts) with the tape (placed on the H1 to H12 side of the block) facing away from each other and turn on at full power for 30 seconds. Rotate the blocks so that the tapes face towards each other and turn on at full power again for 30 seconds.

[0139] Immediately remove the blocks from the microwave and add 300 μl of sterile ice cold H2O with the Multidrop. Seal the blocks with foil tape and place them in an H2O/ice bath.

[0140] Vortex the blocks on 5 for 15 seconds and leave them in the H2O/ice bath. Return to step 7 until all the blocks are in the ice water bath. Incubate the blocks for 15 minutes on ice. Spin the blocks for 30 minutes in the Beckman GS-6KR with GH3.8 rotor with Microplus carrier at 3250 rpm.

[0141] Transfer 100 μl of the supernatant to Corning/Costar round bottom 96 well trays. Cover with foil and put into fridge if to be sequenced right away. If not to be sequenced in the next day, freeze them at −20° C.

[0142] Dye Primer Sequencing: Spin down the DP brew trays and DNA template by pulsing in the Beckman GS-6KR with GH3.8 rotor with Microplus carrier. Big Dye Primer reaction mix trays (one 96 well cycleplate (Robbins) for each nucleotide), 3 microliters of reaction mix per well.

[0143] Use twelve channel pipetter (Costar) to add 2 μl of template to one each G,A,T,C, trays for each template plate. Pulse again to get both the reaction mix and template into the bottom of the cycle plate and put them into the MJ Research DNA Tetrad (PTC-225).

[0144] Start program Dye-Primer. Dye-primer is:

[0145] 96° C., 1 min 1 cycle

[0146] 96° C., 10 sec.

[0147] 55° C., 5 sec.

[0148] 70° C., 1 min 15 cycles

[0149] 96° C., 10 sec.

[0150] 70° C., 1 min. 15 cycles

[0151] 4° C. soak

[0152] When done cycling, using the Robbins Hydra 290 add 100 μl of 100% ethanol to the A reaction cycle plate and pool the contents of all four cycle plates into the appropriate well.

[0153] To perform ethanol precipitation: Use Hydra program 4 to add 100 μl 100% ethanol to each A tray. Use Hydra program 5 to transfer the ethanol and therefore combine the samples from plate to plate. Once the G, A, T, and C trays of each block are mixed, spin for 30 minutes at 3250 in the Beckman. Pour off the ethanol with a firm shake and blot on a paper towel before drying in the speed vac (˜10 minutes or until dry). If ready to load add 3 μl dye and denature in the oven at 95° C. for ˜5 minutes and load 2 μl. If to store, cover with tape and store at −20° C.

[0154] Common Solutions

[0155] Terrific Broth

[0156] Per liter:

[0157] 900 ml H2O

[0158] 12 g bacto tryptone

[0159] 24 g bacto-yeast extract

[0160] 4 ml glycerol

[0161] Shake until dissolved and then autoclave. Allow the solution to cool to 60° C. or less and then add 100 ml of sterile 0.17M KH2PO4, 0.72M K2HPO4 (in the hood w/ sterile technique).

[0162] 0.17M KH2PO4, 0.72M K2HPO4

[0163] Dissolve 2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 ml of H2O.

[0164] Adjust volume to 100 ml with H2O and autoclave.

[0165] Sequence loading Dye

[0166] 20 ml deionized formamide

[0167] 3.6 ml dH2O

[0168] 400 μl 0.5M EDTA, pH 8.0

[0169] 0.2 g Blue Dextran

[0170] *Light sensitive, cover in foil or store in the dark.

[0171] STET/TWEEN

[0172] 10 ml 5M NaCl

[0173] 5 ml 1M Tris, pH 8.0

[0174] 1 ml 0.5M EDTA., pH 8.0

[0175] 25 ml Tween20

[0176] Bring volume to 500 ml with H2O

[0177] The sequencing reactions are run on an ABI 377 sequencer per manufacturer's' instructions. The sequencing information obtained each run are analyzed as follows.

[0178] Sequencing reads are screened for ribosomal., mitochondrial., chloroplast or human sequence contamination. In good sequences, vector is marked by x's. These sequences go into biolims regardless of whether or not they pass the criteria for a ‘good’ sequence. This criteria is >=100 bases with phred score of >=20 and 15 of these bases adjacent to each other.

[0179] Sequencing reads that pass the criteria for good sequences are downloaded for assembly into consensus sequences (contigs). The program Phrap (copyrighted by Phil Green at University of Washington, Seattle, Wash.) utilizes both the Phred sequence information and the quality calls to assemble the sequencing reads. Parameters used with Phrap were determined empirically to minimize assembly of chimeric sequences and maximize differential detection of closely related members of gene families. The following parameters were used with the Phrap program to perform the assembly:

2Penalty−6Penalty for mismatches (substitutions)Mismatch40Minimum length of matching sequence touse in assembly of readsTrim penalty0penalty used for identifying degeneratesequence at beginning and end of read.Minscore80Minimum alignment score

[0180] Results from the Phrap analysis yield either contigs consisting of a consensus of two or more overlapping sequence reads, or singlets that are non-overlapping.

[0181] The contig and singlets assembly were further analyzed to eliminate low quality sequence utilizing a program to filter sequences based on quality scores generated by the Phred program. The threshold quality for “high quality” base calls is 20. Sequences with less than 50 contiguous high quality bases calls at the beginning of the sequence, and also at the end of the sequence were discarded. Additionally, the maximum allowable percentage of “low quality base calls in the final sequence is 2%, otherwise the sequence is discarded.

[0182] The stand-alone BLAST programs and Genbank databases were downloaded from NCBI for use on secure servers at the Paradigm Genetics, Inc. site. The sequences from the assembly were compared to the GenBank NR database downloaded from NCBI using the gapped version (2.0) of BLASTX. BLASTX translates the DNA sequence in all six reading frames and compares it to an amino acid database. Low complexity sequences are filtered in the query sequence. (Altschul et al. (1997) Nucleic Acids Res 25(17):3389-402).

[0183] Genbank sequences found in the BLASTX search with an E Value of less than 1e−10 are considered to be highly similar, and the Genbank definition lines were used to annotate the query sequences.

[0184] When no significantly similar sequences were found as a result of the BLASTX search, the query sequences were compared with the PROSITE database (Bairoch, A. (1992) PROSITE: A dictionary of sites and patterns in proteins. Nucleic Acids Research 20:2013-2018. ) to locate functional motifs.

[0185] Query sequences were first translated in six reading frames using the Wisconsin GCG pepdata program (Wisconsin Package Version 10.0, Genetics Computer Group (GCG), Madison, Wis., USA. ). The Wisconsin GCG motifs program (Wisconsin Package Version 10.0, Genetics Computer Group (GCG), Madison, Wis., USA.) used to locate motifs in the peptide sequence, with no mismatches allowed. Motif names from the PROSITE results were used to annotate these query sequences.

3TABLE 1SEQ IDReferenceAnnotation 120230010 >emb|CAB10331.1| (Z97339) pyruvate, orthophosphate dikinase[Arabidopsis thaliana] Length = 960 220230021E-169 >sp|O02654|ENO_LOLPE ENOLASE (2-PHOSPHOGLYCERATEDEHYDRATASE) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE)>gi|1911573|bbs|175625 (S80961) enolase [Loligo pealii = squids, nervous system,Peptide, 434 aa] [Loligo pealei] Length = 434 320230030 >gi|1669387 (U41998) actin 2 [Arabidopsis thaliana] Length = 377 420230041E-10 >sp|P44677|TOLB_HAEIN TOLB PROTEIN PRECURSOR>gi|1073946|pir|F64064 colicin tolerance protein (to|B) homolog - Haemophilusinfluenzae (strain Rd KW20) >gi|1573352 (U32722) colicin tolerance protein (to|B)[Haemophilus influenzae Rd] Length = 427 520230050 >gi|2062164 (AC001645) jasmonate inducible protein isolog[Arabidopsis thaliana] Length = 470 620230060 >emb|CAA20523.1| (AL031369) Protein phosphatase 2C-like protein[Arabidopsis thaliana] >gi|4559345|gb|AA023006.1|AC006585_1 (AC006585)protein phosphatase 2C [Arabidopsis thaliana] Length = 355 720230070 >sp|P31167|ADTI _ARATH ADP,ATP CARRIER PROTEIN 1 PRECURSOR(ADP/ATP TRANSLOCASE 1) (ADENINE NUCLEOTIDE TRANSLOCATOR 1)(ANT 1) >gi|99658|pir|S21313 ADP,ATP carrier protein - Arabidopsis thaliana(fragment) >gi|16175|emb|CAA46518| (X65549) adenylate translocator[Arabidopsis thaliana] >gi|445607|prf|1909354A adenylate translocator[Arabidopsis thaliana] Length = 379 820230080 >sp|P29517|TBB9_ARATH TUBULIN BETA-9 CHAIN >gi|320190|pir|JQ1593tubulin beta-9 chain - Arabidopsis thaliana >gi|166910 (M84706) beta-9 tubulin[Arabidopsis thaliana] >gi|5262779|emb|CAB45884.1|(AL080282) tubulin beta-9chain [Arabidopsis thaliana] Length = 444 920230090 >pir||S71288 magnesium chelatase chain - Arabidopsis thaliana>gi|1154627|emb″CAA92802| (Z68495) magnesium chelatase subunit[Arabidopsis thaliana] Length = 1381 1020230101E-133 >sp|P92966|RS41_ARATH ARGININE/SERINE-RICH SPLICINGFACTOR RSP41 >gi|1707370|emb|CAA67799| (X99436) splicing factor[Arabidopsis thaliana] Length = 356 1120230110 >dbj|BAA11682| (D83025) proline oxidase precursor [Arabidopsisthaliana] Length = 499 1220230120 >sp|P176I4|ATP2_NICPL ATP SYNTHASE BETA CHAIN,MITOCHONDRIAL PRECURSOR >gi|82133|pir||A24355 H+-transporting ATPsynthase (EC 3.6.1.34) beta-1 chain, mitochondrial - curled-leaved tobacco>gi|19685|emb|CAA26620| (X02868) ATP synthase beta subunit [Nicotianaplumbaginifolia] Length = 560 1320230130 >gi|2160158 (AC000132) Similar to elongation factor 1-gamma(gb|EF1G_XENLA). ESTs gb|T20564,gb|T45940,gb|T04527 come from this gene.[Arabidopsis thaliana] Length = 414 142023014Rgd(2092-2094) 1520230150 >sp|P49676|BGAL_BRAOL BETA-GALACTOSIDASE PRECURSOR(LACTASE) >gi|1076460|pir|S52393 beta-galactosidase (EC 3.2.1.23) - wildcabbage >gi|669059|emb|CAA59162| (X84684) beta-galactosidase [Brassicaoleracea] Length = 828 1620230160>pir|S08534 translation elongation factor eEF-1 alpha chain (gene A4)- Arabidopsis thaliana >gi|295789|emb|CAA344561 (X16432) elongation factor 1-alpha [Arabidopsis thaliana] Length = 449 1720230172E-68 >gi|4091806 (AF052585) CONSTANS-like protein 2 [Malusdomestica] Length = 329 1820230180 >sp|024456|GBLP_ARATH GUANINE NUCLEOTIDE-BINDING PROTEINBETA SUBUNIT-LIKE PROTEIN (WD-40 REPEAT AUXIN-DEPENDENTPROTEIN ARCA) >gi|2289095 (U77381) WD-40 repeat protein [Arabidopsisthaliana] Length = 327 1920230191 E-140 >sp|Q03460|GLSN_MEDSA GLUTAMATE SYNTHASE [NADH]PRECURSOR (NADH-GOGAT) >gi|484529|pir|JQ1977 glutamate synthase(NADH) (EC 1.4.1.14) - alfalfa >gi|166412 (L01660) NADH-glutamate synthase[Medicago sativa] Length [32 [0 2194 2020230201 E-159 >gi|2677828 (U93166) cysteine protease [Prunus armeniaca]Length [32 [0 358 2120230213E-74 >sp|P3|167|AD1_ARATH ADP,ATP CARRIER PROTEIN 1PRECURSOR (ADP/ATP TRANSLOCASE 1) (ADENINE NUCLEOTIDETRANSLOCATOR 1) (ANT 1) >gi|99658|pir|S21313 ADP,ATP carrier protein -Arabidopsis thaliana (fragment) >gi|16175|emb|CAA46518| (X65549) adenylatetranslocator [Arabidopsis thaliana] >gi|445607|prf|1909354A adenylatetranslocator [Arabidopsis thaliana] Length = 379 2220230221E-136 >pir||S71265 ferritin - Arabidopsis thaliana>gi|124640|emb|CAA63932| (X94248) ferritin [Arabidopsis thaliana] Length = 255 2320230230 >sp|P2S856|GI3PA_ARATH GLYCERALDEHYDE 3-PHOSPHATEDEHYDROGENASE A, CHLOROPLAST PRECURSOR >gi|2117520|pir||JQ1285glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) (EC1.2.1.13) A precursor, chloroplast - Arabidopsis thaliana >gi|166704 (M64117)glyceraldehyde 3-phosphate dehydrogenase [Arabidopsis thaliana] >gi|402885|emb|CAA66816| (X98130) glyceraldehyde-3-phosphatedehydrogenase (NADP+) (phosphorylating) [Arabidopsis thaliana] Length = 396 242023024Tyr_Phospho_Site(1382-1388) 2520230250 >gi|2062167 (AC001645) Proline-rich protein APG isolog [Arabidopsisthaliana] Length = 322 2620230260 >gi|3834314 (AC005679) Similar to gene pi010 glycosyltransferasegi|2257490 from S. pombe clone 1750 gb|AB004534. ESTs gb|T46079 andgb|AA394466 come from this gene. [Arabidopsis thaliana] Length = 405 2720230270 >sp|P25856|G3PA_ARATH GLYCERALDEHYDE 3-PHOSPHATEDEHYDROGENASE A, CHLOROPLAST PRECURSOR >gi|2117520|pir||JQ1285glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) (EC1.2.1.13) A precursor, chloroplast - Arabidopsis thaliana >gi|166704 (M64117)glyceraldehyde 3-phosphate dehydrogenase [Arabidopsis thaliana]>gi|1402885|emb|CAA66816| (X98130) glyceraldehyde-3-phosphatedehydrogenase (NADP+) (phosphorylating) [Arabidopsis thaliana] Length = 396 2820230281E-170 >pir||UQMUM ubiquitin precursor - Arabidopsis thaliana>gi|17678|emb|CAA31331| (X12853) polyubiquitin (AA 1-382) [Arabidopsisthaliana] >gi|987519 (U33014) polyubiquitin [Arabidopsis thaliana]>gi|226499|prf||1515347A poly-ubiquitin [Arabidopsis thaliana] Length = 382 2920230293E-71 >sp|P37707|B2_DAUCA B2 PROTEIN >gi|322726|pir||S32124 B2protein - carrot >gi|297889 |emb|CAA51078| (X72385) B2 protein [Daucus carota]Length = 207 3020230300 >sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINEHYDROLYZING] (GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)>gi|507946 (L29083) glutamine-dependent asparagine synthetase [Arabidopsisthaliana] >gi|5541701|emb|CAB51206.1| (AL096860) glutamine-dependentasparagine synthetase [Arabidopsis thaliana] Length = 584 3120230312E-25 >gb|AAD24193.1|AF134238_1 (AF134238) PL6 protein [Mus musculus]Length = 350 3220230321E-149 >sp|P04778|CB22_ARATH CHLOROPHYLL A-B BINDING PROTEIN2 PRECURSOR (LHCII TYPE I CAB-2) (CAB-140) (LHCP)>gi|16376|emb|CAA27543| (X03909) chlorophyll a/b binding protein (LHCP AB140) [Arabidopsis thaliana] Length = 267 3320230331E-153 >gi|1915974 (U62329) fructokinase [Lycopersicon esculentum]>gi|2102693 (U64818) fructokinase [Lycopersicon esculentum] Length = 328 3420230341E-106 >sp|Q64516|GLPK_MOUSE GLYCEROL KINASE (ATP:GLYCEROL3-PHOSPHOTRANSFERASE) (GLYCEROKINASE) (GK) >gi|1480469 (U48403)glycerol kinase [Mus musculus] Length = 524 3520230351E-103 >emb|CAA16745.1| (AL021711) heat shock transcription factor-likeprotein [Arabidopsis thaliana] Length = 401 3620230361E-170 >gi|2286153 (AF007581) cytoplasmic malate dehydrogenase[Zea mays] Length = 332 372023037Tyr_Phospho_Site (1338-1344) 3820230381E-179 >sp|P19456|PMA2_ARATH PLASMA MEMBRANE ATPASE 2(PROTON PUMP) >gi|67973|pir||PXMUP2 H+-transporting ATPase (EC 3.6.1.35)type 2, plasma membrane - Arabidopsis thaliana >gi|166629 (J05570) H+-ATPase[Arabidopsis thaliana] >gi|5730129|emb|CAB52463.1| (AL109796) H+-transportingATPase type 2, plasma membrane [Arabidopsis thaliana] Length = 948 392023039Pkc_Phospho_Site(5-7) 402023040Tyr_Phospho_Site(830-837) 4120230418E-98 >gi|4204274 (AC004146) ribulose bisphosphate carboxylase,small subunit [Arabidopsis thaliana] Length = 180 4220230421E-175 >emb|CAB38206| (AL035601) auxin-responsive GH3-like protein[Arabidopsis thaliana] Length = 603 432023043Pkc_Phospho_Site(19-21) 4420230449E-58 >sP|P26599|PTB_HUMAN POLYPYRIMIDINE TRACT-BINDINGPROTEIN (PTB) (HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN I)(HNRNP I) (57 KD RNA-BINDING PROTEIN PPTB-1) >gi|3576|emb|CAA43973|(X62006) polypirimidine tract binding protein [Homo sapiens]>gi|35774|emb|CAA43056| (X60648) polypyrimidine tract-binding protein (pPTB)[Homo sapiens] >gi|409606| (AC006273) PTB_HUMAN; PTB;HETEROGENEOUS NUCLEA; HNRNP I; 57 KD RNA-BINDING PROTEIN PPTB1 [Homo sapiens] Length = 531 4520230452E-79 >gi|2642429 (AC002391) poly(A)-binding protein [Arabidopsis thaliana]Length = 662 4620230460 >sp|Q38854|CLA1_ARATH PROBABLE 1-DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE PRECURSOR (DXP SYNTHASE) >gi|1399261(U27099) DEE [Arabidopsis thaliana] Length = 717 472023047Wd_Repeats(1245-1259) 4820230481E-151 >dbj|BAA25181| (D88537) delta 9 desaturase [Arabidopsisthaliana] Length = 307 4920230491E-167 >emb|CAB43488.1| (AJ012278) ATP-dependent Clp proteasesubunit ClpP [Arabidopsis thaliana] >gi|5360579|dbj|BAA82065.1| (AB022326)nC|pP1 [Arabidopsis thaliana] Length = 298 5020230500 >emb|CAA67339| (X98807) peroxidase ATP21a [Arabidopsisthaliana] Length = 329 5120230510 >gb|AAD39650.1|AC007591_15 (AC007591) Similar to gb|Z70524 PDR5-likeABC transporter from Spirodela polyrrhiza and is a member of the PF|00005 ABCtransporter family. ESTs gb|N97039 and gb|T43169 come from this gene. [Arabid 5220230525E-52 >sp|P41227|ARDH_HUMAN N-TERMINAL ACETYLTRANSFERASECOMPLEX ARD1 SUBUNIT HOMOLOG >gi|517485|emb|CAA54691| (X77588)ARD1 N-acetyl transferase homologue [Homo sapiens] >gi|1302661 (U52112)ARD1 N-acetyl transferase related protein [Homo sapiens] Length = 235 5320230531E-126 >gi|3158476 (AF067185) aguaporin 2 [Samanea saman] Length =287 5420230541E-173 >gi|3212877 (AC004005)Lea-like protein [Arabidopsis thaliana]Length = 325 5520230551E-14 >sp|Q28955|PNAD_PIG PROTEIN N-TERMINAL ASPARAGINEAMIDOHYDROLASE (PROTEIN NH2-TERMINAL ASPARAGINE DEAMIDASE)(NTN-AMIDASE) (PNAD) (PROTEIN NH2-TERMINAL ASPARAGINEAMIDOHYDROLASE) (PNAA) >gi|1082956|pir||A55768 asparaginyl-peptideamidohydrolase (EC 3.5.1.-) - pig>gi|595950 (U17062) protein N-terminalasparagine amidohydrolase [Sus scrofa] Length = 310 5620230561E-172 >sp|P53799|FDFT_ARATH FARNESYL-DIPHOSPHATEFARNESYLTRANSFERASE (SQUALENE SYNTHETASE) (SQS) (SS) (FPP:FPPFARNESYLTRANSFERASE) >gi|1076324|pir||554251 farnesyl-diphosphatefarnesyltransferase (EC 2.5.1.21)- Arabidopsis thaliana>gi |798820|emb|CAA60385| (X86692) farnesyl-diphosphate farnesyltransferase[Arabidopsis thaliana] >gi|806325|dbj|BAA06103| (D29017) squalene synthase[Arabidopsis thaliana] >gi|2232212 (AF004560) squalene synthase 1 [Arabidopsisthaliana] >gi|3096933|emb|CAA| 8843.1| (AL023094) farnesyl-diphosphatefarnesyltransferase [Arabidopsis thaliana] >gi|4098519 (U79159) squalenesynthase [Arabidopsis thaliana] Length = 410 5720230571E-141 >gi|3413700 (AC004747) YME1 protein [Arabidopsis thaliana]Length = 627 582023058Tyr_Phospho_Site(1667-1673) 5920230591E-144 >sp|Q08682|RSP4_ARATH 40S RIBOSOMAL PROTEIN SA (P40)(LAMININ RECEPTOR HOMOLOG) >gi|322536|pir||530570 laminin receptorhomolog - Arabidopsis thaliana >gi|16380|emb|0AA487941 (X69056) lamininreceptor homologue [Arabidopsis thaliana] Length = 298 6020230602E-43 >gi|2735550 (U96638) unc-50 related protein; URP [Rattusnorvegicus] Length = 259 612023061Tyr_Phospho_Site (65-73) 6220230622E-30 >emb|CAB03470.1| (Z81137) Similarity to Yeast YIP1 protein(SW:P53039); cDNA EST EMBL:T01608 comes from this gene; cDNA ESTEMBL:C07393 comes from this gene; cDNA EST EMBL:C07550 comes from thisgene; cDNA EST EMBL:C08746 comesfrom this gene . . . Length = 282 6320230631E-151 >gi|1773330 (U80071) glycolate oxidase [Mesembryanthemumcrystallinum] Length = 370 6420230647E-44 >ref|NP_006339.1|PGTC90| Golgi transport complex protein (90 kDa)>gi|3808235 (AF058718) 13 S Golgi transport complex 90kD subunit brain-specific isoform [Homo sapiens] Length = 839 6520230651E-168 >emb|CAB44681| (AL078620) mitochondrial carrier-like protein[Arabidopsis thaliana] Length = 330 6620230664E-12 >gi|1764100 (U81805) GDP-D-mannose-4,6-dehydratase[Arabidopsis thaliana] Length = 373 6720230672E-22 >gb|AAD48936.1|AF160760_4 (AF160760) contains similarity to Pfamfamily PF0040 - WD domain, G-beta repeat; score = 10.8, E = 3.2, N-2 [Arabidopsisthaliana] Length = 892 6820230681E-123 >sp|P30302|WC2C_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 2C (WATER-STRESS INDUCED TONOPLAST INTRINSIC PROTEIN)(WSI-TIP) >gi|217869|dbj|BAA02520|(D13254) transmembrane channel protein[Arabidopsis thaliana] >gi|4371283|gb|AAD18141| (AC006260) plasma membraneintrinsic protein 2C [Arabidopsis thaliana] >gi|384324|prf||1905411Atransmembrane channel [Arabidopsis thaliana] Length = 285 6920230696E-12 >dbj|BAA74463| (AB022605) mRNA (guanine-7-)methyltransferase[Homo sapiens] Length = 504 7020230701E-153 >gi|206216| (AC001645) jasmonate inducible protein isolog[Arabidopsis thaliana] Length = 298 7120230711E-157 >sp|P43286|WC2A_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 2A >gi|629542|pir|1544084 plasma membrane intrinsic protein 2a -Arabidopsis thaliana >gi|472877|emb|CAA53477| (X75883) plasma membraneintrinsic protein 2a [Arabidopsis thaliana] Length = 287 7220230729E-98 >gi|2252840 (AF013293) contains regions of similarity toHaemophilus influenzae permease (SP:P38767) [Arabidopsis thaliana]>gi|604988|gb|AAF02797.1|AF195115_17 (AF195115) contains regions ofsimilarity to Haemophilus influenzae permease (SP:P38767) [Arabidopsis thaliana]Length = 746 7320230739E-97 >gb|AAF00673.11AC008153_25 (AC008153) 2-cys peroxiredoxin BAS1precursor (thiol-specific antioxidant protein) [Arabidopsis thaliana]>gi|6041816|gb|AAF02131.1|AC0099|8_3 (AC009918) 2-cys peroxiredoxin [Arab 7420230741E-168 >emb|CAA06460| (AJ005261) cytidine deaminase [Arabidopsisthaliana] >gi|3093276|emb|CAA06671.1| (AJ005687) cytidine deaminase[Arabidopsis thaliana] >gi|4191787 (AC005917) cytidine deaminase [Arabidopsisthaliana] >gi|6090835|gb|AAF03358.| AF 134487_1 (AF134487) cytidinedeaminase 1 [Arabidopsis thaliana] Length = 301 7520230750 >emb|CAA66863| (X98190) peroxidase ATP2a [Arabidopsis thaliana]>gi|4371288|gb|AA018146| (AC006260) peroxidase ATP2a [Arabidopsis thaliana]Length = 327 7620230761E-159 >sp|Q08733|WC1C_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 1C (TRANSMEMBRANE PROTEIN B) (TMP-B)>gi|396218|emb|CAA491551 (X69294) transmembrane protein TMP-B[Arabidopsis thaliana] Length = 286 772023077Rgd(840-842) 7820230781E-157 >emb|CAB10405.1| (Z97340) beta-1, 3-glucanase class I precursor[Arabidopsis thaliana] Length = 306 7920230791E-110 >gi|3341679 (AC003672) dynamin-like protein phragmoplastin12 [Arabidopsis thaliana] Length = 613 8020230801E-79 >gb|AAA02747.1| (L13655) membrane protein [Saccharum hybridcultivar H65-7052] Length = 325 8120230811E-155 >pir||S33443 chlorophyll a/b-binding protein CP29 - Arabidopsisthaliana >gi|298036|emb|CAA50712| (X71878) CP29 [Arabidopsis thaliana] Length =290 8220230820 >emb|CAB56580.1| (AJ011628) squamosa promoter binding protein-like1 [Arabidopsis thaliana] Length = 881 832023083Tyr_Phospho_Site(305-312) 8420230846E-22 >gb|AAD46141.1|AF081022_1 (AF081022) hypoxia-induced proteinkinase L31 [Lycopersicon esculentum] Length = 78 8520230851E-154 >gi|2281109 (AC002333) endochitinase isolog [Arabidopsisthaliana] Length = 281 8620230861E-79 >gi|3415117 (AF081203) villin 3 [Arabidopsis thaliana] Length =966 8720230871E-103 >ref|NP_005435.1 |PRODI+| protein involved in sexual development>gi||1620898|dbj|BAA13508| (D87957) protein involved in sexual development[Homo sapiens] Length = 299 8820230881E-106 >5p|Q05047|CP72_CATRO CYTOCHROME P450 72A1 (CYPLXXII)(PROBABLE GERANIOL-10-HYDROXYLASE) (GE10H) >gi—167484 (L10081)Cytochrome P-450 protein [i Catharanthus roseus] >gi|445604|prf||1909351Acytochrome P450 [Catharanthus roseus] Length = 524 8920230895E-41 >ref|NP000511.1|PHEXA| hexosaminidase A (alpha polypeptide)>gi|123079|5p|P06865|HEXA_HUMAN BETA-HEXOSAMINIDASE ALPHA CHAINPRECURSOR (N-ACETYL-BETA-GLUCOSAMINIDASE) (BETA-N-ACETYLHEXOSAMINIDASE) >gi|67503|pir|AOHUBA beta-N-acetylhexosaminidase (EC 3.2.1.52) alpha chain precursor - human >gi|179458(M16424) beta-hexosaminidase alpha chain [Homo sapiens]>gi|4261632|gb|AAD13932|1680052_1 (S62076) lysosomal enzyme beta-N-acetylhexosaminidase A [Homo sapiens]Length = 529 9020230900 >emb|CAB36796.1| (AL035525) pectinesterase-like protein [Arabidopsisthaliana] Length = 477 9120230911E-139 >emb|CAB10240.1| (Z97336) disease resistance RPS2 like protein[Arabidopsis thaliana] Length = 719 9220230921E-170 ) >pir||S49332 seed tetraubiquitin - common sunflower>gi|303901|dbj|BAA03764| (D16248) ubiquitin [Glycine max]>gi|456714|dbj|BAA05670| (D28123) Ubiquitin [Glycine max]>gi|556688|emb|CAA84440| (Z34988) seed tetraubiquitin [Helianthus annuus]>gi|994785|dbj|BAA05085| (D26092) Ubiquitin [Glycine max]>gi|4263514|gb|AAD15340| (AC004044) polyubiquitin [Arabidopsis thaliana]>gi|1096513|prf|2111434A tetraubiquitin [Helianthus annuus] Length = 305 9320230931E-146 >gi|2088652 (AF002109) 26S proteasome regulatory subunitS12 isolog [Arabidopsis thaliana] >gi|2351376 (U54561) translation initiation factoreIF2 p47 subunit homolog [Arabidopsis thaliana] Length = 293 9420230940 >pir||B45511 chitinase (EC 3.2.1.14) precursor, basic - Arabidopsisthaliana >gi|166666 (M38240) basic chitinase [Arabidopsis thaliana]>gi|5689104|dbj|BAA82811.1| (AB023449) basic endochitinase [Arabidopsisthaliana] >gi|5689106|dbj|BAA82812.1| (AB023450) basic endochitinase[Arabidopsis thaliana] >gi|5689108|dbj|BAA82813.1| (AB023451) basicendochitinase [Arabidopsis thaliana] >gi|5689112|dbj|BAA82815.1| (AB023453)basic endochitinase [Arabidopsis thaliana] >gi|5689114|dbi|BAA82816.1|(AB023454) basic endochitinase [Arabidopsis thaliana]>gi|5689120|dbi|BAA82819.1| (AB023457) basic endochitinase [Arabidopsisthaliana] >gi|5689122|dbj|BAA82820.| (AB023458) basic endochitinase[Arabidopsis thaliana] >gi|5689124|dbj|BAA82821.1| (AB023459) basicendochitinase [Arabidopsis thaliana] >gi|5689126|dbj|BAA82822.| (AB023460)basic endochitinase [Arabidopsis thaliana] >gi|5689128|dbi|BAA82823.|(AB023461) basic endochitinase [Arabidopsis thaliana]>gi|5689132|dbj|BAA82825.1| (AB023463) basic endochitinase [Arabidopsisthaliana] Length = 335 952023095Tyr_Phospho_Site(1027-1033) 9620230961E-152 >pir||523546 chlorophyll a/b-binding protein type I precursorLhb1B2 - Arabidopsis thaliana >gi|16364|emb|CAA45790| (X64460) photosystemII type I chlorophyll a/b binding protein [Arabidopsis thaliana] >gi|3128230(AC004077) photosystem II type I chlorophyll a/b binding protein [Arabidopsisthaliana] >gi|3337371 (AC004481) photosystem II type I chlorophyll a/b bindingprotein [Arabidopsis thaliana] Length = 265 972023097Tyr_Phospho_Site(98-104) 9820230981E-133 >emb|CAB38813.1| (AL035679) ubiquitin-dependent proteolyticprotein [Arabidopsis thaliana] Length = 315 9920230995E-45 >gb|AAD26911.1|AC0064299 (AC006429) auxin down-regulated protein[Arabidopsis thaliana] Length = 29110020231001E-169 >sp|P46523|CLPA_BRANA ATP-DEPENDENT CLP PROTEASEATP-BINDING SUBUNIT CLPA PRECURSOR >gi|480969|pir|S37557 clpAprotein - rape (fragment) >gi|406311|emb|CAA53077| (X75328) clpA [Brassicanapus] Length = 87410120231011E-100 >gb|AAD28780.1|AF134133_1 (AF134133) Lil3 protein [Arabidopsisthaliana] Length = 26210220231024E-42 >gi|3329368 (AF031244) nodulin-like protein [Arabidopsisthaliana] Length = 5591032023103Tyr_Phospho_Site(206-212)1042023104Tyr_Phospho_Site(740-748)10520231051E-130 >pir||520866 L-ascorbate peroxidase (EC 1.11.1.11) precursor- Arabidopsis thaliana (fragment) Length = 26310620231062E-15 >gi|4093153 (AF088280) phytochrome-associated protein 3[Arabidopsis thaliana] Length = 5241072023107Zinc Protease(1292-1301)10820231081E-148 ) >dbj|BAA32735| (AB011545) GF14 mu [Arabidopsis thaliana]>gi|4559343|gb|AAD23005.1|AC007087_24 (AC007087) DNA regulatory proteinGF14 mu [Arabidopsis thaliana] >gi|5802796|gb|AAD51784.1|AF145301_1(AF145301) 14-3-3 protein GF14 mu [Arabidopsis thaliana] Length = 2631092023109Zinc Finger_C3hc4(138-147)11020231102E-49 >dbj|BAA|6833| (D90901) spore germination protein c2[Synechocystis sp.] Length = 23811120231112E-44 >emb|CAA21916.1| (AL033389) yeast cell division cycle CDC50homolog [Schizosaccharomyces pombe] Length = 3961122023112Zinc Finger C2h2(879-903)11320231133E-66 >gb|AAD39835.1|AF0570249 (AF057024) Ran-binding protein siRanBP[Arabidopsis thaliana] Length = 23411420231141E-173 >9b|AAD38248.1|AC0061934 (AC006193) membrane related protein[Arabidopsis thaliana] Length = 3851152023115Tyr_Phospho_Site(1708-1714)11620231165E-63 >emb|CAA69300| (Y08061) endomembrane-associated protein[Arabidopsis thaliana] >gi|2982443|emb|CAA18251.1| (AL022224)endomembrane-associated protein [Arabidopsis thaliana] Length = 22511720231172E-46 >gi|451193 (L28008) wali7 [Triticum aestivum]>gi|1090845|prf||2019486B wali7 gene [Triticum aestivum] Length = 27011820231181E-102 >pir||S58499 IAA13 protein -Arabidopsis thaliana >gi|972929(U18415)IAA13 [Arabidopsis thaliana] >gi|2459414 (AC002332) auxin inducibleprotein, IAA13 [Arabidopsis thaliana] Length = 2461192023119Tyr_Phospho_Site(14-21)12020231201E-147 >sp|P27521|CB24_ARATH CHLOROPHYLL A-B BINDING PROTEIN4 PRECURSOR (LHCI TYPE III CAB-4) (LHCP) >gi|166646 (M63931) light-harvesting chlorophyll a/b binding protein [Arabidopsis thaliana] Length = 2511212023121Tyr_Phospho_Site(414-421)12220231223E-59 >emb|CAB10557.1| (Z97344) trehalose-6-phosphate synthase likeprotein [Arabidopsis thaliana] Length = 8651232023123Tyr_Phospho_Site(110-117)12420231241E-109 >dbj|BAA33810.1| (AB018441) phi-1 [Nicotiana tabacum] Length =31312520231251E-120 >emb|CAB56038.1| (AJ011049) tyrosine decarboxylase[Arabidopsis thaliana] Length = 4891262023126Tyr_Phospho_Site(640-647)12720231273E-44 >ref|NP005818.1|PUGTREL1| UDP-galactose transporter related>gi|2136346|pir||JC5024 UDP-galactose transporter related isozyme 1 - human>gi|1669560|dbj|BAA13525.1| (D87989) UGTrel1 [Homo sapiens] Length = 32212820231281E-115 >sp|P42055|POR4_SOLTU 34 KD OUTER MITOCHONDRIALMEMBRANE PROTEIN PORIN (VOLTAGE-DEPENDENT ANION-SELECTIVECHANNEL PROTEIN) (VDAC) (POM 34) >gi|629720|pir||S46936 34K porin -potato >gi|1076682|pir||A55364 porin (clone pPOM-34) - potato mitochondrion>gi|516166|emb|CAA56599| (X80386) 34 kDA porin [Solanum tuberosum] Length =2761292023129Tyr_Phospho_Site(25-32)13020231301E-76 >sp|Q42656|AGAL_COFAR ALPHA-GALACTOSIDASE PRECURSOR(MELIBIASE) (ALPHA-D-GALACTOSIDE GALACTOHYDROLASE) >gi|504489(L27992) alpha-galactosidase [Coffea arabica] Length = 37813120231312E-20 >gb|AAF0|440.1|AF187961| (AF187961) ubiquitin carboxyl-terminalhydrolase [Schizosaccharomyces pombe] Length = 112913220231321E-141 >emb|CAA17567| (AL021961) caffeoyl-CoA O-methyltransferase- like protein [Arabidopsis thaliana] Length = 25913320231331E-97 >emb|CAA64565| (X95269) LRR protein [Lycopersicon esculentum]Length = 22113420231343E-53 >dbj|BAA24576| (AB000778) phospholipase D [Rattus norvegicus]Length = 107413520231359E-48 >sp|P27061|PPA1_LYCES ACID PHOSPHATASE PRECURSOR 1>gi|170370 (M83211) acid phosphatase type 1 [Lycopersicon esculentum]>911170372 (M67474) acid phosphatase type 5 [Lycopersicon esculentum]>gi|445121|prf||1908427A acid phosphatase 1 [Lycopersicon esculentum] Length =25513620231361E-138 ) >gi|3421072 (AF043519) 205 proteasome subunit PAA2[Arabidopsis thaliana] >gi|4Q06819|gb|AAC95161.1| (AC005970) 20S proteasomesubunit PAA2 [Arabidopsis thaliana] Length = 24613720231372E-75 >gb|AAD|4602| (AF092910) stage specific peptide 24[Trypanosoma cruzi] Length = 28713820231381E-158 >pir|1559519 tryptophan synthase (EC 4.2.1.20) alpha chain -Arabidopsis thaliana >gi|619753 (U18993) tryptophan synthase alpha chain[Arabidopsis thaliana] >gi|1585768|prf||2201482A Trp synthase:SUBUNIT = alpha[Arabidopsis thaliana] Length = 3121392023139Tyr_Phospho_Site(892-900)14020231403E-53 >emb|CAB43522.1| (AJ238804) non-specific lipid transfer protein[Arabidopsis thaliana] Length = 11814120231411E-1 65 >pir|1571226 xyloglucan endotransglycosylase-related proteinXTR-7 - Arabidopsis thaliana >gi|1244760 (U43489) xyloglucanendotransglycosylase-related protein [Arabidopsis thaliana] Length = 28914220231421E-146 >9b|AAD55272.1|AC008263_3 (AC008263) Identical to gb|AF078080isochorismate synthase from Arabidopsis thaliana. ESTs gb|R90272, gb|A1100274and gb|T42189 come from this gene. Length = 50314320231431E-158 >sp|P43285|WC1A_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 1A >gi|629540|pir||S44082 plasma membrane intrinsic protein 1a -Arabidopsis thaliana >gi|472873|emb|CAA534751 (X75881) plasma membraneintrinsic protein 1a [Arabidopsis thaliana] Length = 28614420231441E-173 >sp|Q38882|PLD_ARATH PHOSPHOLIPASE D PRECURSOR (PLD)(CHOLINE PHOSPHATASE) (PHOSPHATIDYLCHOLINE-HYDROLYZINGPHOSPHOLIPASE D) >gi|1297302 (U36381) phospholipase D [Arabidopsisthaliana] Length = 80914520231453E-97 >sp|Q03943|IM30_PEA CHLOROPLAST MEMBRANE-ASSOCIATED30 KD PROTEIN PRECURSOR (M30) >gi|1076532|pir|S47966 probable lipidtransfer protein M30 precursor - garden pea >gi|169107 (M73744) IM30 [Pisumsativum] Length = 32314620231461E-167 >sp|P5S737|HS82_ARATH HEAT SHOCK PROTEIN 81-2 (HSP81-2)>gi|445127|prf||1908431B heat shock protein HSP81-2 [Arabidopsis thaliana]Length = 6991472023147Pkc_Phospho_Site(56-58)14820231483E-26 >dbj|BAA759l 9.11 (AB009340) tartrate-resistant acid phoshatase[Oryctolagus cuniculus] Length = 32514920231491E-159 >emb|CAA17774.1| (AL022023) plasma membrane intrinsic protein(SIMIP) [Arabidopsis thaliana] Length = 28015020231501E-155 >gi|2443883 (AC002294) Similar to RPS-2 disease resistanceprotein [Arabidopsis thaliana] Length = 96715120231511E-99 >gb|AAD29800.1|AC006264_8 (AC006264) signal sequence receptor,alpha subunit (SSR-alpha) [Arabidopsis thaliana] Length = 2571522023152Tyr_Phospho_Site(642-650)15320231532E-64 >gb|AAC78271.1|AAC78271 (AC002330) glutamate-/aspartate-bindingpeptide [Arabidosis thaliana] Length = 24815420231541E-172>gi|4218963 (AF093672) xyloglucan endotransglycossylase[Arabidopsis thaliana] >gi|4539300 |emb|CAB39603.1| (AL049480) xyloglucanendo-1,4-beta-D-glucanase [Arabidopsis thaliana] Length = 2871552023155Zinc_Finger_C2h2(917-941)15620231561E-108 >emb|CAA65416| (X96598) CaLB protein [Arabidopsis thaliana]Length = 49315720231575E-26 >emb|CAA64425| (X94976) cell wall-plasma membrane linkerprotein [Brassica napus] Length = 37615820231581E-159 >gb|AAD25750.1|AC007060_8 (AC007060) Strong similarity to F1913.7gi|3033380 coatomer epsilon subunit from Arabidopsis thaliana BACgb|AC004238. ESTs gb|Z17908, gb|AA728673, gb|N96555, gb|H76335,gb|AA712463, gb|W43247, gb|T4561 1, g . . . Length = 2921592023159Tyr_Phospho_Site(958-964)16020231605E-14 >emb|CAA18475.1| (AL022347) serine /threonine kinase-like protein,receptor kinase [Arabidopsis thaliana] Length = 65616120231613E-33 >sp|P26568|H11_ARATH HISTONE H1.1 >gi|1070594|pir||HSMU11histone H1.1 - Arabidopsis thaliana >gi|16317|emb|CAA44314| (X62458) HistoneH1 [Arabidopsis thaliana] Length = 27416220231622E-97 >emb|CAA07573.1| (AJ007586) src2-like protein [Arabidopsisthaliana] Length = 3241632023163Tyr_Phospho_Site(246-254)16420231641E-171 >sp|Q42547|CAT3_ARATH CATALASE 3 >gi|2347178 (U43147)catalase 3 [Arabidopsis thaliana] >gi|251 1726 (AF021937) catalase 3 [Arabidopsisthaliana] Length = 4921652023165Tyr_Phospho_Site(75-83)16620231661E-151 >emb|CAA66966| (X98322) peroxidase [Arabidopsis thaliana]>gi|1429219|emb|CAA67312| (X98776) peroxidase ATP13a [Arabidopsis thaliana]Length = 31316720231677E-38 >emb|CAB41106.1| (AL049656) myb-like protein [Arabidopsisthaliana] Length = 26116820231688E-74 >gi|4008006 (AF084034) receptor-like protein kinase [Arabidopsisthaliana] Length = 64516920231691E-137 >pir||JQ1678 transcription factor tga1 - Arabidopsis thaliana>gi|16550|emb|CAA481891 (X68053) transcription factor [Arabidopsis thaliana]Length = 36717020231708E-57 >gi|3184559 (AF052290) c-type cytochrome biogenesis protein[Synechococcus PCC7002] Length = 24617120231711E-103 ) >gb|AAD32768.1|AC007661_5 (AC007661) alpha-carboxyltransferase[Arabidopsis thaliana] Length = 79617220231721E-117 >gb|AAD32822.1|AC0076594 (AC007659) phosphatidatecytidylyltransferase [Arabidopsis thaliana] Length = 43017320231731E-129 >dbj|BAA32210| (AB015138) Vacuolar proton pyrophosphatase[Arabidopsis thaliana] Length = 77017420231742E-76 >gi|3157927 (AC002131) Contains similarity to GDP-dissociationinhibitor gb|L07918 from Mus musculus. [Arabidopsis thaliana] Length = 22317520231752E-89 >pir||S68589 serine/threonine-specific kinase (EC 2.7.1.-)precursor - Arabidopsis thaliana >gi|1405837|emb|CAA62824| (X91630) receptor-like kinase [Arabidopsis thaliana] >gi|2150023 (AF001168) receptor-like kinaseLECRK1 [Arabidopsis thaliana] Length = 66117620231767E-86 >gi|3769673 (AF095285) Tic20 [Pisum sativum] Length = 25317720231772E-17 >sp|P46689|GAS1_ARATH GIBBERELLIN-REGULATED PROTEIN 1PRECURSOR >gi|2129588|pir|157144| GAST1 protein homolog (clone GASA1)- Arabidopsis thaliana >gi|887939 (U11766) GAST1 protein homolog [Arabidopsisthaliana] Length = 9817820231781E-166 >sp|048661|SPEE_ARATH SPERMIDINE SYNTHASE(PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)>gi|2821 961|dbj|BAA24536| (AB006693) spermidine synthase [Arabidopsisthaliana Length = 2931792023179Ww_Domain_1(1284-131018020231801E-104 >pir||S27762 Sip1 protein - barley >gi|167100 (M77475) seedimbibition protein [Hordeum vulgare] Length = 75718120231811E-155 >sp|P48641|GSHR_ARATH GLUTATHIONE REDUCTASE,CYTOSOLIC (GR) (GRASE) (OBP29) >gi|1022797 (U37697) glutathionereductase [Arabidopsis thaliana] Length = 4991822023182Tyr_Phospho_Site(599-607)18320231831E-133 >gi|3688799 (AF057137) gamma tonoplast intrinsic protein 2[Arabidopsis thaliana] Length = 25318420231841E-110 >gi|3075392 (AC004484) steroid dehydrogenase [Arabidopsisthaliana] Length = 3901852023185Tyr_Phospho_Site(48-56)18620231866E-38 >emb|CAAl6875.1| (AL021749) receptor protein kinase like protein1872023187Tyr_Phospho_Site 1737-174318820231881E-128 >sp|P48349|143L_ARATH 14-3-3-LIKE PROTEIN GF14 LAMBDA (14-3-3-LIKE PROTEIN AFT1)>gi|1084332|pir|S53727 14-3-3-like protein (AFT1)-Arabidopsis thaliana>gi|953321 (UO2565) 14-3-3-like protein 1 [Arabidopsisthaliana] >gi|1549404 (U68545) GF14 lambda [Arabidopsis thaliana]>gi|5802790|gb|AAD51781.1|AF145298_1 (AF145298) 14-3-3 protein GF14lambda [Arabidopsis thaliana] Length = 24818920231891E-135 >emb|CAB39932.1| (AL049500) phosphoribosylanthranilatetransferase [Arabidopsis thaliana] Length = 8571902023190Serpin(1794-1804)19120231913E-77 >gi|3319340 (AF077407) contains similarity to E. coli cationtransport protein ChaC (GB:D90756) [Arabidopsis thaliana] Length = 19719220231921E-47 >emb|CAA23033.1| (AL035394) major latex protein [Arabidopsisthaliana] Length = 15119320231937E-76 >gb|AAB17191.1| (U73103) laccase [Liriodendron tulipifera] Length =5701942023194Tyr_Phospho_Site(712-718)19520231951E-161 >sp|Q06611|WC1B_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 1B (TRANSMEMBRANE PROTEIN A) (TMP-A)>gi|296085|emb|0AA48356| (X68293) transmembrane protein [Arabidopsisthaliana] >gi|3386599 (AC004665) plasma membrane intrinsic protein 1B[Arabidopsis thaliana] Length = 28619620231961E-16 >sp|P44445|RLUD_HAEIN RIBOSOMAL LARGE SUBUNITPSEUDOURIDINE SYNTHASE D (PSEUDOURIDYLATE SYNTHASE) (URACILHYDROLYASE) >gil|074296|pir|F64144 hypothetical protein H10176 -Haemophilus influenzae (strain Rd KW20) >gi|1573131 (U32702) sfhB protein(sfhB) [Haemophilus influenzae Rd] Length = 32419720231972E-22 >gb|AAD48964.1|AF1472636 (AF147263) contains similarity to Medicagotruncatula N7 protein (GB:Y17613) [Arabidopsis thaliana] Length = 2461982023198Tyr_Phospho_Site 1422-14281992023199Tyr_Phospho_Site(1517-1524)20020232001E-109 >gi|2642432 (AC002391) elicitor response element bindingprotein (WRKY3) [Arabidopsis thaliana] Length = 3172012023201Tyr_Phospho_Site(271-279)20220232021E-176 ) >gi|3599968 (AF032123) clp protease [Arabidopsis thaliana]Length = 3102032023203Tyr_Phospho_Site(964-971)20420232041E-127 >emb|CAA04386| (AJ000886) Tetrafunctional protein ofglyoxysomal fatty acid beta-oxidation [Brassica napus] Length = 72520520232054E-32 >emb|CAA04124| (AJ000486) methionine gamma-lyase[Trichomonas vaginalis ] Length = 3962062023206SE-61 >pir||S66770 probable membrane protein YOL077c - yeast(Saccharomyces cerevisiae) >gi|1419909|emb|CAA99087| (Z74819) ORFYOL077c [Saccharomyces cerevisiae] Length = 29120720232071E-127 >emb|CAA66785| (X98108) 23 kDa polypeptide of oxygen-evolving comlex (OEC) [Arabidopsis thaliana] Length = 26320820232081E-131 >gb|AAF00659.1|AC008153_11 (AC008153) cell division related protein[Arabidopsis thaliana] Length = 66320920232091E-141 >sp|P11574|VATB_ARATH VACUOLAR ATP SYNTHASE SUBUNITB (V-ATPASE B SUBUNIT) (V-ATPASE 57 KD SUBUNIT) >gi|81637|pir||A31886H+-transporting ATPase (EC 3.6.1.35) 57K chain - Arabidopsis thaliana>gi|166627 (J04185) nucleotide-binding subunit of vacuolar ATPase [Arabidopsisthaliana] Length = 49221020232103E-45 >gi|3242706 (AC003040) cyclin-dependent kinase inhibitorprotein [Arabidopsis thaliana] >gi|3550262 (AF079587) cyclin-dependent kinaseinhibitor; ICK1 [Arabidopsis thaliana] Length = 19121120232111E-140 >gb|AAD28777.1|AF134130_1 (AF134130) Lhcb6 protein [Arabidopsisthaliana] Length = 25821220232121E-151 ) >sp|P29511|TBA6_ARATH TUBULIN ALPHA-6 CHAIN>gi|282852|pir||JQ1597 tubulin alpha-6 chain - Arabidopsis thaliana >gi|166920(M84699) TUA6 [Arabidopsis thaliana] >gi|2244853|emb|CAB10275.11 (Z97337)tubulin alpha-6 chain (TUA6) [Arabidopsis thaliana] Length = 4502132023213Tyr_Phospho_Site(405-412)21420232141E-175 ) >emb|CAB16823.1| (Z99708) aminopeptidase-like protein[Arabidopsis thaliana] Length = 63421520232152E-33 >emb|CABI 30471 (Z99110) yjcL [Bacillus subtilis] Length = 39621620232161E-143 >sp|Q05466|HAT4_ARATH HOMEOBOX-LEUCINE ZIPPERPROTEIN HAT4 (HD-ZIP PROTEIN 4) (HD-ZIP PROTEIN ATHB-2)>gi|629516|pir||S31424 homeotic protein Athb-2 - Arabidopsis thaliana>gi|16180|emb|CAA48246| (X68145) Athb-2 [Aribido21720232171E-149 >emb|CAA72487| (Y11791) peroxidase ATP26a [Arabidopsisthaliana] Length = 2762182023218Tyr_Phospho_Site(404-411)21920232191E-138 >gi|2262167 (AC002329) cytosolic ribosomal protein S4[Arabidopsis thaliana] Length = 26122020232201E-163 >gb|AAD30579.1|AC007260_10 (AC007260) Similar to dTDP-D-glucose4,6-dehydratase [Arabidopsis thaliana] Length = 66922120232210 ) >pir||SS2150 serine O-acetyltransferase (EC 2.3.1.30) - Arabidopsisthaliana >gi|2146776|pir||S67482 serine O-acetyltransferase (EC 2.3.1.30) -Arabidopsis thaliana >gi|608577 (L34076) serine acetyltransferase [Arabidopsisthaliana] >gi|608677|emb|CAA84371| (Z34822220232221E-116 >emb|CAB42903.| (AL049862) UTP-glucose glucosyltransferaselike protein [Arabidopsis thaliana] Length = 47822320232231E-46 >emb|CAB10538.2| (Z97343) TEGT protein homolog [Arabidopsisthaliana] Length = 2622242023224Tyr_Phospho_Site(1002-1010)22520232251E-117 >gi|2583121 (AC002387) phosphotransferase [Arabidopsisthaliana] Length = 2572262023226Tyr_Phospho_Site(732-738)2272023227Tyr_Phospho_Site(1093-1100)22820232283E-24 >gb|AAD236S1.11AC007119 _17 (AC007119) glycine-rich RNA bindingprotein Ccr2 [Arabidopsis thaliana] Length = 17922920232291E-145 >dbj|BAA342S0| (AB013886) RAV1 [Arabidopsis thaliana] Length= 34423020232301E-142 >emb|CAB43855.1| (AL078465) isp4 like protein [Arabidopsisthaliana] Length = 75323120232314E-89 >gi|2252866 (AF013294) contains region of similarity to SYT[Arabidopsis thaliana] Length = 23023220232323E-27 >dbj|BAA83740.1| (AB023288) TRAB1 [Oryza sativa] Length = 3182332023233Tyr_Phospho_Site(919-926)2342023234Tyr_Phospho_Site(1189-1196)2352023235Tyr_Phospho_Site(301-307)23620232361E-168 >gb|AADS6290.1|AF162279_1 (AF162279) 10-formyltetrahydrofolatesynthetase [Arabidopsis thaliana] Length = 63423720232371E-112 >gi|3738320 (AC005170) cinnamoyl CoA reductase[Arabidopsis thaliana] Length = 30323820232381E-18 >emb|CAA23041.1| (AL035394) Ap2 domain protein [Arabidopsisthaliana] l Length = 3432392023239Tyr_Phospho_Site(393-401)24020232404E-22 >gi|699154 (U15180) P450 cytochrome,isopentenyltransf,ferridox. [Mycobacterium leprae] Length = 18724120232411E-131 >sp|P24636|TBB4_ARATH TUBULIN BETA-4 CHAIN>gi|2129546|pir||S68122 beta-tubulin 4 - Arabidopsis thaliana >gi|166640(M21415) beta-tubulin [Arabidopsis thaliana] Length = 44424220232421E-112 ) >gi|3790581 (AF079179) RING-H2 finger protein RHB1a[Arabidopsis thaliana] Length = 19024320232431E-124 >emb|CAA55006| (X78116) Acetoacetyl-coenzyme A thiolase[Raphanus sativus] Length 40624420232447E-11 >gi|2622337 (AE000890) inosine-540 -monophosphatedehydrogenase related protein V [Methanobacterium thermoautotrophicum]Length = 18724520232453E-11 >emb|CAB45565.1| (AL079355) phospholipase C [Streptomycescoelicolor] Length = 5012462023246Tyr_Phospho_Site(1121-1127)24720232471E-148 >pir||525677 chlorophyll a/b-binding protein type I precursorLhb1B1 - Arabidopsis thaliana >gi|16366|emb|CAA45789| (X64459) photosystemII type I chlorophyll a /b binding protein [Arabidopsis thaliana] >gi|3128229(AC004077) photosystem II type I chlorophyll a/b binding protein [Arabidopsisthaliana] >gi|3337372 (AC004481) photosystem II type I chlorophyll a/b bindingprotein [Arabidopsis thaliana] Length 26624820232481E-113 >gi|3941466 (AF062887) transcription factor [Arabidopsisthaliana] Length = 35224920232493E-18 >gb|AAD42398.1|AF157493_6 (AF157493)carboxymethylenebutenolidase [Zymomonas mobilis] Length = 3102502023250Tyr_Phospho_Site(663-671)2512023251Tyr_Phospho_Site(648-655)25220232521E-138 ) >gb|AAC62791.1| (AF096371) contains similarity to D-isomerspecific 2-hydroxyacid dehydrogenases (Pfam: 2-Hacid_DH.hmm, score: 19.11)[Arabidopsis thaliana] Length = 6622532023253Tyr_Phospho_Site(984-990)25420232541E-130 >sp|P42737|CAH2_ARATH CARBONIC ANHYDRASE 2(CARBONATE DEHYDRATASE 2) >gi|438449 (L18901) carbonic anhydrase[Arabidopsis thaliana] Length = 25925520232551E-135 >emb|CAB39787.1 | (AL049488) chlorophyll a/b-binding protein-like[Arabidopsis thaliana] >gi|4741958|gb|AAD28776.1|AF134129_1 (AF134129)Lhcb5 protein [Arabidopsis thaliana] Length = 2802562023256Tyr_Phospho_Site(1564-1570)25720232571E-140 ) >gi|3264805 (AF071788) phosphoenolpyruvate carboxylaseArabidopsis thaliana >gi|4079630|emb|CAA10486| AJ131710 phospho enolepyruvate carboxylase [Arabidopsis thaliana] Length = 96825820232581E-111 >emb|CAB10530.1| (Z97343) EREBP-4 like protein [Arabidopsisthaliana] Length = 60325920232591E-127 >sp|P48491|TPIS_ARATH TRIOSEPHOSPHATE ISOMERASE,CYTOSOLIC (TIM) >gi|414550 (U02949) cytosolic triose phosphate isomerase[Arabidopsis thaliana] >gi|742408|prf||2009415A triose phosphate isomerase[Arabidopsis thaliana] Length = 2542602023260Tyr_Phospho_Site(963-969)26120232611E-152 ) >emb|CAB36755.1 | (AL035523) protein-methionine-S-oxidereductase [Arabidopsis thaliana] Length = 2582622023262Tyr_Phospho_Site(1080-1087)26320232631E-140 >sp|Q38997|K110_ARATH SNF1-RELATED PROTEIN KINASE KIN10(AKIN10) >gi|322596|pir||JC1446 serine/threonine protein kinase (EC 2.7.-.-) AK21- Arabidopsis thaliana >gi|166600 (M93023) SNF1-related protein kinase[Arabidopsis thaliana] >gil| 742969|emb|CAA64384| (X94757) ser/thr proteinkinase [Arabidopsis thaliana] Length = 51226420232641E-158 >gb|AAD28774.1|AF134127_| (AF134127) Lhcb4.2 protein [Arabidopsisthaliana] Length = 2872652023265Tyr_Phospho_Site(370-377)26620232661E-173 >gb|AAD25800.1|AC006550_8 (AC006550) Identical to gb|U12536 3-methylcrotonyl-CoA carboxylase precursor protein from Arabidopsis thaliana.ESTs gb|H35836, gb″AA651295 and gb|AA721862 come from this gene. Length =7302672023267Tyr_Phospho_Site(861-867)26820232681E-131 >gi|3941522 (AF062915) transcription factor [Arabidopsisthaliana] Length = 24926920232691E-147 >9b|AAB53256.1| (U66408) GTP-binding protein [Arabidopsisthaliana] >gi|2345150|gb|AAB678301 (AF014822) developmentally regulated GTPbinding protein [Arabidopsis thaliana] Length = 3992702023270Tyr_Phospho_Site(786-793)27120232711E-133 >gi|3746809 (AF082882) adenylate kinase [Arabidopsis thaliana]Length = 24627220232723E-91 >emb|CAA71277| (Y10228) P-glycoprotein-2 [Arabidopsis thaliana]>gi|2108254|emb|CAA712761 (Y10227) P-glycoprotein-2 [Arabidopsis thaliana]>gi|4538925|emb|0AB39661.11 (AL049483) P-glycoprotein-2 (pgp2) [Arabidopsisthaliana] Length = 123327320232731E-107 >gi|1353352 (U31975) alanine aminotransferase[Chlamydomonas reinhardtii] Length = 52127420232747E-84 >emb|CAA23040.1| (AL035394) receptor kinase [Arabidopsisthaliana] Length = 63827520232751E-129 >gi|1145697 (U39485) delta tonoplast integral protein[Arabidopsis thaliana] Length = 25027620232761E-54 >emb|CAA96657.1| (Z72511) possible zinc finger protein; cDNA ESTEMBL:M89115 comes from this gene; cDNA EST EMBL:D71 533 comes from thisgene; cDNA EST EMBL:D72314 comes from this gene; cDNA EST EMBL:D75164comes from this gene; cDNA EST EMBL: . . . Length = 6102772023277Pkc_Phospho_Site(73-75)27820232781E-154 >gi|3335374 (AC003028) glutaredoxin-like protein [Arabidopsisthaliana] Length = 29327920232791E-128 >gbjAAD57005.1|AC009465_19 (AC009465) 40S ribosomal protein S3A(S phase specific) [Arabidopsis thaliana] Length = 26228020232801E-114 >9b|AAD28778.1|AF1341311 (AF134131) PsbS protein [Arabidopsisthaliana] Length = 26528120232817E-62 >gb|AAD25756.1|AC007060_14 (AC007060) Contains the PF|00650CRAL/TRIO phosphatidyl-inositol-transfer protein domain. ESTs gb|T76582,gb|N06574 and gb|Z25700 come from this gene. [Arabidopsis thaliana] Length =54028220232820 >sp|P25851|F16P_ARATH FRUCTOSE-1,6-BISPHOSPHATASE,CHLOROPLAST PRECURSOR (D-FRUCTOSE-1,6-BISPHOSPHATE 1-PHOSPHOHYDROLASE) (FBPASE) >gi|99693|pir||S16582 fructose-bisphosphatase (EC 3.1.3.11) precursor, chloroplast -Arabidopsis thaliana>gi|11242|emb|CAA41154═ (X58148) fructose-bisphosphatase [Arabidopsisthaliana] Length = 41728320232831E-162 >gi|4220476 (AC006069) ribophorin I-like protein [Arabidopsisthaliana] Length = 46428420232841E-151 >pir||UQPM ubiquitin precursor - garden pea>gi|20589|emb|CAA34886| (X17020) polyubiquitin (AA 1-381) [Pisum sativum]>gi|4115339 (L81142) ubiquitin [Pisum sativum] >gi|226707|prf||1603402A poly-ubiguitin [Pisum sativum] Length = 3812852023285Rgd(1319-1321)28620232861E-143 >gi|3980379 (AC004561) cyclin, PCNA [Arabidopsis thaliana]Length = 26428720232871E-108 >gb|AAF00071.1|AF093604_1 (AF093604) apyrase [Arabidopsisthaliana] Length = 47128820232888E-99 >sp|P36397|ARF1_ARATH ADP-RIBOSYLATION FACTOR 1>gi|322518|pir| |S28875 ADP-ribosylation factor 1 - Arabidopsis thaliana2892023289Tyr_Phospho_Site(570-577)2902023290Zinc Finger C3hc4(177-186)2912023291Pkc_Phospho_Site(23-25)29220232921E-146 ) >emb|CAB43632.1| (AL050351) SEC14-like protein [Arabidopsisthaliana] Length = 61729320232931E-109 >sp|P46422|GTH4_ARATH GLUTATHIONE S-TRANSFERASE PM24(24 KD AUXIN-BINDING PROTEIN) (GST CLASS PHI) >gi|479736|pir||535268glutathione transferase (EC 2.5.1.18) gst2- Arabidopsis thaliana >gi|166723(L07589) glutathione 5-transferase [Arabidopsis thaliana] >gi|347212 (L11601)glutathione 5-transferase [Arabidopsis thaliana] >gi|407090|emb|CAA53051|(X75303) glutathione S-transferase [Arabidopsis thaliana]>gi|2262152|gb|AAC78264.1|AAC78264 (AC002330) Atpm24.1 glutathione Stransferase [Arabidopsis thaliana] Length = 21229420232943E-21 >emb|CAA22977.1| (AL035353) photosystem I subunit PSI-E-likeprotein [Arabidopsis thaliana] >gi|5732203|emb|CAB52678.1| (AJ245908)photosystem I subunit IV precursor [Arabidopsis thaliana] Length = 1432952023295Tyr_Phospho_Site(441-447)29620232961E-159 >gi|166835 (M86720) ribulose bisphosphatecarboxylase/oxygenase activase [Arabidopsis thaliana] >gi|2642170 (AC003000)Rubisco activase [Arabidopsis thaliana] Length = 4462972023297Tyr_Phospho_Site(757-764)29820232981E-22 >gi|4102690 (AF004806) 24 kDa seed maturation protein [Glycinemaxi Length = 2122992023299Tyr_Phospho_Site(366-373)30020233001E-142 >gi|4056500 (AC005896) acetyltransferase [Arabidopsisthaliana] Length = 43230120233015E-68 >emb|CAAQ7236| (AJ006771) beta-galactosidase [Cicer arietinum]Length = 70730220233021E-104 >sp|P52577||FRH_ARATH ISOFLAVONE REDUCTASE HOMOLOGP3 >gi|1361992|pir||S57613 isoflavonoid reductase homolog- Arabidopsis thaliana>gi 18864321emb 1CAA898591 (Z49777) isoflavonoid reductase homologue[Arabidopsis thaliana30320233031E-123 >gb|AAD20405| (A0007019) ATP synthase [Arabidopsisthaliana] Length = 24030420233041E-131 >dbj|BAA32418| (AB008103) ethylene responsive element bindingfactor 1 [Arabidopsis thaliana] Length = 26630520233051E-142 >dbj|BAA78560.1| (AB024282) cysteine synthase [Arabidopsisthaliana] >gi|5824334|emb|CAB54830.| (AJ010505) cysteine synthase[Arabidopsis thaliana] Length = 3683062023306Tyr_Phospho_Site(92-100)30720233072E-79 >emb|CAB42925.1| (AL049862) tRNA synthetase [Arabidopsisthaliana] Length = 22530820233083E-25 >gb|AAD46141.1|AF0810221 (AF081022) hypoxia-induced protein L31[Lycopersicon esculentum] Length = 7830920233091E-110 >emb|CAA16677| (AL021684) LRR-like protein [Arabidopsisthaliana] Length = 44531020233108E-38 >dbj|BAA22374| (D86122) Mei2-like protein [Arabidopsis thaliana]Length = 88431120233111E-135 >gb|AAD32291.1|AC006533_15 (AC006533) acetolactate synthase[Arabidopsis thaliana] Length = 48431220233122E-98 >gb|AAB51567.11 (U75189) germin-like protein [Arabidopsisthaliana] >gi|1755158|gb|AAB51568.1| (U75190) germin-like protein [Arabidopsisthaliana] >gi|1755170|gb|AAB51574.1| (U75196) germin-like protein [Arabidopsisthaliana] >gi|1755172|gb|AAB51575.1| (U75197) germin-like protein [Arabidopsisthaliana] >gi|1755180|gb|AAB51579.1| (U75201) germin-like protein [Arabidopsisthaliana] >gi|1755190|gb|AAB51584.1| (U75206) germin-like protein [Arabidopsisthaliana] >gi|1934728|gb|AAB51751.1| (U95035) germin-like protein [Arabidopsisthaliana] >gi|4154285 (AF090733) germin-like protein 1 [Arabidopsis thaliana]>gi|4666248|dbj|BAA77207.1| (D89055) germin-like protein precursor [Arabidopsisthaliana] Length = 2083132023313Pkc_Phospho_Site(14-16)3142023314Pkc_Phospho_Site(92-94)31520233151E-119 >emb|CAA96434| (Z71 752) pectin methylesterase [Nicotianaplumbaginifolia] Length = 31531620233161E-130 ) >sp|O237O8|PRC3_ARATH PROTEASOME COMPONENT C3(MACROPAIN SUBUNIT C3) (MULTICATALYTIC ENDOPEPTIDASE COMPLEXSUBUNIT C3) >gi|2511574|emb|CAA73619.1| (Y13176) multicatalyticendopeptidase [Arabidopsis thaliana] >gi|3421075 (AF043520) 20S proteasomesubunit PAB1 [Arabidopsis thaliana] >gi|4966368|gb|AA034699.1|AC006341_27(AC006341) Identical to gb|Y13176 Arabidopsis thaliana mRNA for proteasomesubunit prc3. ESTs gb|H36972, gb|T22551 and gb|T13800 come from this gene.Length = 2353172023317Pkc_Phospho_Site(11-13)3182023318Tyr_Phospho_Site(1345-1353)3192023319Tyr_Phospho_Site(309-315)32020233201E-115 >gi|2829275 (AF044265) nucleoside diphosphate kinase 3[Arabidopsis thaliana] >gi|35l 3740 (AFO80118) contains similarity to nucleosidediphosphate kinases (Pfam: NDK.hmm, score: 301.12) [Arabidopsis thaliana]>gi|4539375|emb|CAB40069.1| (AL049525) nucleoside diphosphate kinase 3(ndpk3) [Arabidopsis thaliana] Length = 23832120233211E-160 >sp|P42498|PHYE_ARATH PHYTOCHROME E>gi|1076376|pir||S46313 phytochrome E- Arabidopsis thaliana>gi|452817|emb|CAA54075| (X76610) phytochrome E [Arabidopsis thaliana]>gi|5816999|emb|CAB53654.1| (AL110123) phytochrome E [Arabidopsis thaliana]Length = 111232220233221E-35 >gb|AAD28506.1|AF123265| (AF123265) remorin 1 [Lycopersiconesculentum] Length = 19732320233231E-171 >gi|4220452 (AC006216) Similar to 9113413714 T19L18.21myrosinase-binding protein from Arabidopsis thaliana BAC gb AC004747. ESTsgb|T44298, gb|T42447, gb|R64761 and gb|1100206 come from this gene.[Arabidopsis thaliana] Length = 29232420233243E-21 >pir||S62011 PH085 protein - yeast (Saccharomyces cerevisiae)>gi|1163103 (U43503) Lph16p [Saccharomyces cerevisiae] Length = 122332520233254E-59 >sp|P73839|THDFSYNY3 POSSIBLE THIOPHENE AND FURANOXIDATION PROTEIN THDF >gi|1652979|dbj|BAA178961 (D90910) thiophen andfuran oxidation protein [Synechocystis sp.] Length = 45632620233261E-117 >emb|CAA17161| (AL021890) calcium-dependent protein kinase- like protein [Arabidopsis thaliana] >gi|2961339|emb|CAA18097.1| (AL022140)calcium-dependent protein kinase-like protein [Arabidopsis thaliana] Length = 55432720233271E-105 >gi|3980412 (AC004561) pumilio-like protein [Arabidopsisthaliana] Length = 96832820233281E-160 ) >dbj|BAA82066.1 | (AB022327) nClpP2 [Arabidopsis thaliana]Length = 27932920233291E-129 ) >emb|CAA041721 (AJ000539) phosphatidylinositol synthase[Arabidopsis thaliana] Length = 22733020233308E-65 >gb|AAD11598.1|AAD11598 (AF071527) calcium channel [Arabidopsisthaliana] >gi|4263043|gb|AAD15312| (AC005142) calcium channel [Arabidopsisthaliana] Length = 7243312023331Tyr_Phospho_Site(46-53)33220233321E-126 >gi|2981475 (AF053084) cinnamyl alcohol dehydrogenase[Malus domestica] Length = 3253332023333Tyr_Phospho_Site(126-132)33420233341E-142 >emb|CAB39936.1| (AL049500) osmotin precursor [Arabidopsisthaliana] Length 24433520233351E-138 >gb|AAD28767.1|AF134120_1 (AF134120) Lhca2 protein [Arabidopsisthaliana] Length = 2573362023336Tyr_Phospho_Site(628-636)33720233373E-14 >sp|P34092|MYSB_DICDI MYOSIN IB HEAVY CHAIN>gi|102252|pir||A33284 myosin heavy chain lB - slime mold (Dictyosteliumdiscoideum) >gi|167839 (M26037) myosin I heavy chain [Dictyosteliumdiscoideum] Length = 111133820233382E-68 >sp|P37707|B2_DAUCA B2 PROTEIN >gi|322726|pir|1532124 B2protein - carrot >gi|297889|emb|CAA51078| (X72385) B2 protein [Daucus carota]Length = 20733920233391E-146 ) >gi|3980402 (AC004561) tropinone reductase [Arabidopsisthaliana] Length = 26034020233401E-68 >dbj|BAA11226| (D78151) human 26S proteasome subunit p97[Homo sapiens] Length = 90834120233411E-117 >sp|P51430|RS6_ARATH 40S RIBOSOMAL PROTEIN S6>gi|2224751|emb|CAA74381| (Y14052) ribosomal protein 56 [Arabidopsisthaliana] Length = 24934220233421E-109 >emb|CAA|7550| (AL021961) receptor protein kinase - likeprotein [Arabidopsis thaliana] Length = 98034320233431E-106 >sp|Q42599|NUIM_ARATH NADH-UBIQUINONEOXIDOREDUCTASE 23 KD SUBUNIT PRECURSOR (COMPLEX 1-23KD) (Cl-23KD) >gi|1076356|pir||552380 NADH dehydrogenase (EC 1.6.99.3)- Arabidopsisthaliana >gi|666977|emb|CAA59061| (X8431 8) NADH dehydrogenase[Arabidopsis thaliana] >gi|3152573 (AC002986) Match to NADH:ubiquinoneoxidoreductase gb|X84318 from A. thaliana. ESTs gb|Z27005, gb|T04711,gb|T45078 and gb|Z28689 come from this gene. [Arabidopsis thaliana] Length =22234420233441E-142 ) >gi|3763918 (AC004450) isopropylmalate dehydratase[Arabidopsis thaliana] Length = 25134520233455E-84 >sp|P54641|VATX_DICDI VACUOLAR ATP SYNTHASE SUBUNITAC39 (V-ATPASE AC39 SUBUNIT) (41 KD ACCESSORY PROTEIN) (DVA41)>gi|626048|pir||A55016 lysosomal membrane protein DVA41 - slime mold(Dictyostelium discoideum) >gi|532733 (U13150) vacuolar ATPase subunit DVA41[Dictyostelium discoideum] Length = 35634620233465E-88>gb|AAD15451| (AC006068) receptor protein kinase [Arabidopsisthaliana] Length = 56734720233471E-61 >sp|P3|3166|APT1_ARATH ADENINEPHOSPHORIBOSYLTRANSFERASE 1 (APRT) >g|199657|pir||S20867 adeninephosphoribosyltransferase (EC 2.4.2.7)- Arabidopsis thaliana>gi|16164|emb|CAA41497| (X58640) adenine phosphoribosyltransferase[Arabidopsis thaliana] >gi|433050 (L19637) adenine phosphoribosyltransferase[Arabidopsis thaliana] >gi|3935182 (AC004557) F17L21.25 [Arabidopsis thaliana]Length = 18334820233481E-127 >emb|CAA10060.1| (AJ012571) glutathione transferase[Arabidopsis thaliana] Length = 2193492023349Pkc_Phospho_Site(28-30)35020233501E-123 >gi|3201613 (AC004669) glutathione S-transferase [Arabidopsisthaliana] Length = 21535120233511E-109 >sp|P51119|GLN2_VITVI GLUTAMINE SYNTHETASE CYTOSOLICISOZYME 2 (GLUTAMATE-AMMONIA LIGASE) >gi|1134898|emb|CAA63982|(X94321) glutamine synthetase [Vitis vinifera] Length = 35635220233522E-23 >gi|871782 (L43081) pEARLI 4 gene product [Arabidopsisthaliana] Length = 76635320233531E-150 >emb|CAA66963| (X98319) peroxidase [Arabidopsis thaliana]>gi|1429217|emb|CAA6731| (X98775) peroxidase ATP12a [Arabidopsis thaliana]Length = 32135420233548E-46 >gi|4206763 (AF104328) cell wall-plasma membrane linkerprotein homolog [Arabidopsis thaliana] Length = 30635520233551E-140 >gi|1644427 (U74610) glyoxalase II [Arabidopsis thaliana]Length = 25635620233561E-158 >gi|3757514 (AC005167) plasma membrane intrinsic protein[Arabidopsis thaliana] >gi|4581129|gb|AAD24619.1|AC005825_26 (AC005825)plasma membrane intrinsic protein [Arabidopsis thaliana] Length = 27835720233571E-139 >gi|2708750 (AC003952) physical impedence protein[Arabidopsis thaliana] Length = 45235820233581E-117 >sp|004157|RAB7_ARATH RAS-RELATED PROTEIN RAB7>gi|2065015|emb|CAA70951| (Y09821) GTP-binding protein Rab7 [Arabidopsisthaliana] >gi|2505866|emb|0AA72904| (Y12227) GTP-binding protein Rab7[Arabidopsis thaliana] >gi|3287684 (AC003979) Strong similaity to gb|Y09821GTP-binding protein Rab7 from A. thaliana. EST gb|T76449 comes from thisgene. [Arabidopsis thaliana] Length = 20335920233593E-20 >gi|3213227 (AF035209) v-SNARE Vtila [Mus musculus]>gi|3421062 (AF035823) 29-kDa Golgi SNARE [Mus musculus] Length = 21736020233602E-25 >dbj|BAA37095.1| (AB022209) ribonucleoprotein F [Rattusnorvegicus] Length = 4153612023361Pkc_Phospho_Site(67-69)36220233626E-78 >gb|AAD25780.1|AC006577_16 (AC006577) Similar to gb|U55861 RNAbinding protein nucleolysin (TIAR) from Mus musculus and contains severalPF|00076 RNA recognition motif domains. ESTs gb|T21032 and gb|T44127 comefrom this gene. [Arabidopsis t . . . Length = 4263632023363Pkc_Phospho_Site(14-16)36420233643E-11 >emb|CAA16558| (AL021635) leucine rich repeat receptor kinase-like protein [Arabidopsis thaliana] Length = 68836520233651E-140 >sp|P34791|CYP4_ARATH PEPTIDYL-PROLYL CIS-TRANSISOMERASE, CHLOROPLAST PRECURSOR (PPIASE) (ROTAMASE)(CYCLOPHILIN) (CYCLOSPORIN A-BINDING PROTEIN)>gi|1076368|pir||B53422 peptidylprolyl isomerase (EC 5.2.1.8) ROC4- Arabidopsisthaliana >911405131 (L14845) cyclophilin [Arabidopsis thaliana] >gi|1322278(U42724) cyclophilin [Arabidopsis thaliana] Length = 26036620233662E-56 >emb|CAA89697| (Z49697) cysteine proteinase inhibitor [Ricinuscommunis] Length = 2093672023367Tyr_Phospho_Site(1552-1558)36820233681E-137 >gi|2252855 (AF013294) similar to the myc family of helix-loop-helix transcription factors [Arabidopsis thaliana] Length = 42336920233691E-103 >sp|P48006|EF1B_ARATH ELONGATION FACTOR 1-BETA A1 (EF-1-BETA) >gi|480620|pir||S37103 translation elongation factor eEF-1 beta-A1 chain- Arabidopsis thaliana (cv. Colombia) >gi|398608|emb|CAA52751| (X74733)elongation factor-1 beta A1 [Arabidopsis thaliana] Length = 23137020233701E-109 >emb|CAA74639| (Y14251) glutathione S-transferase[Arabidopsis thaliana] Length = 2093712023371Rgd(581-583)37220233721E-131 ) >gb|AAD51783.1|AF145300_1 (AF145300) 14-3-3 protein GF14 kappa[Arabidopsis thaliana] Length = 24837320233731E-139 >emb|CAA51171| (X72581) tonoplast intrinsic protein gamma(gamma-TIP) [Arabidopsis thaliana] Length = 2513742023374Tyr_Phospho_Site(1037-1044)37520233751E-126 >emb|CAB10400.1| (Z97340) enoyl-CoA hydratase like protein[Arabidopsis thaliana] Length = 24437620233763E-15 >gb|AAD34107.1|AF151870_1 (AF151870) CGI-112 protein [Homosapiens Length = 20837720233771E-137 >gb|AAD25640.1|AC0071702 (AC007170) cytoplasmic aconitatehydratase [Arabidopsis thaliana] Length = 8983782023378Tyr_Phospho_Site(787-793)37920233791E-123 >sp|P52032|GSHY_ARATH GLUTATHIONE PEROXIDASEHOMOLOG PRECURSOR >gi|2129599|pir≡1|571250 glutathione peroxidase -Arabidopsis thaliana >gil 1061036|emb|CAA6| 9651 (X89866) glutathioneperoxidase [Arabidopsis thaliana] Length = 24238020233803E-99 >gb|AAD25928.1|AF085279| (AF085279) hypothetical Ser-Thr proteinkinase [Arabidopsis thaliana] Length = 57038120233816E-58 >emb|CAB43976.1| (AL078579) zinc finger protein [Arabidopsisthaliana] Length 32738220233821E-132 ) >gi|3421087 (AF043524) 20S proteasome subunit PAE1[Arabidopsis thaliana] >gi|6056394|gbJAAF02858.1|AC009324_7 (AC009324) 20Sproteasome subunit PAE1 [Arabidopsis thaliana] Length = 23738320233832E-14 >emb|CAA92677.1| (Z68315) Similarity to Human MAP kinasephosphatase-1 (SW:PTN7 HUMAN) [Caenorhabditis elegans] Length = 15038420233841E-146 >gb|AAD37165.1|AF132742_| (AF132742) 3-phosphoinositide-dependent protein kinase-1 [Arabidopsis thaliana] Length = 49138520233851E-109 >emb|CAA64820| (X95573) salt-tolerance zinc finger protein[Arabidopsis thaliana] Length = 22738620233861E-169 >gi|3834309 (AC005679) Strong similarity to glycoprotein EP1gb|L16983 Daucus carota and a member of S locus glycoprotein family PF|00954.ESTs gb|F13813, gb|T21052, gb|R30218 and gb|W43262 come from this gene.38720233874E-20 >ref|NP_006283.1|PTSG101| tumor susceptibility gene 101 >gi|3184258(U82130) tumor susceptibility protein [Homo sapiens] Length = 39038820233881E-163 >gi|1046225 (U21952) ethylene response sensor [Arabidopsisthaliana] >9112623308 (AC002409) ethylene response sensor (ERS) [Arabidopsisthaliana] >gi|1584365|prf||2122405A ERS gene [Arabidopsis thaliana] Length =6133892023389Tyr_Phospho_Site(86-93)39020233901E-138 >sp|Q08733|WC1C_ARATH PLASMA MEMBRANE INTRINSICPROTEIN 10 (TRANSMEMBRANE PROTEIN B) (TMP-B)>gi|396218|emb|CAA49155| (X69294) transmembrane protein TMP-B[Arabidopsis thaliana] Length = 28639120233917E-28 >dbj|BAA32422| (AB008107) ethylene responsive element bindingfactor 5 [Arabidopsis thaliana] Length = 30039220233921E-108 >dbj|BAA31509| (AB010877) chloroplast ribosomal protein L3[Nicotiana tabacum] Length = 2593932023393Pkc_Phospho_Site(133-135)3942023394Tyr_Phospho_Site(1037-1043)3952023395Tyr_Phospho_Site(603-609)3962023396Tyr_Phospho_Site(579-586)39720233971E-1-1 >dbj|BAA2S180| (D88536) delta 9 desaturase [Arabidopsisthaliana] Length = 3053982023398Tyr_Phospho_Site(1372-1378)39920233991E-105 >emb|CAB08077| (Z94058) pectinesterase [Lycopersiconesculentum] Length = 50440020234004E-35 >emb|CAA197651 (AL031004) RSZp22 sp|icing factor [Arabidopsisthaliana] >gi|3435094|gb|AAD12769.1| (AF033586) 9G8-like SR protein[Arabidopsis thaliana] Length = 20040120234011E-125) >gi|2191150 (AF007269) similar to mitochondrial carrier family[Arabidopsis thaliana] Length = 35240220234021E-136 >emb|CAA74025.1| (Y13691) multicatalytic endopeptidasecomplex, proteasome component, alpha subunit [Arabidopsis thaliana] Length =24540320234031E-156 >sp|P25697|KPPR_ARATH PHOSPHORIBULOKINASEPRECURSOR (PHOSPHOPENTOKINASE) (PRKASE) (PRk)>gi|99744|pir||516583 phosphoribulokinase (EC 2.7.1.19) precursor- Arabidopsisthaliana >gi|16441|emb|CAA41155| (X58149) Ribulose-5-phosphate kinase[Arabidopsis thaliana] Length = 39540420234041E-90 >dbj|BAA77837.1| (AB027458) ACE [Arabidopsis thaliana]>gi|5903086|gb|AAD55644.1|AC008017_17 (AC008017) ACE [Arabidopsisthaliana] Length = 59440520234051E-98 >dbj|BAA24804| (AB010946) AtRer1B [Arabidopsis thaliana] Length= 1954062023406Tyr_Phospho_Site(120-126)40720234071E-143 >gb|AAD39331.1|AC00725820 (AC007258) pyruvate dehydrogenaseE1 alpha subunit [Arabidopsis thaliana] Length = 3894082023408Tyr_Phospho_Site(593-601)40920234091E-14 >gi|3152583 (AC002986) Contains similarity to inhibitor ofapoptosis protein gb|U4S88l from D. melanogaster. [Arabidopsis thaliana] Length =3474102023410Tyr_Phospho_Site(1596-1603)4112023411Tyr_Phospho_Site(1068-1075)41220234121E-127 >gb|AAD31074.1|AC007357_23 (AC007357) Similar to gb|AF038007FICI gene from Homo sapiens and is a member of the PF100122 E1-E2 ATPasefamily. ESTs gb|T45045 and gb|AA394473 come from this gene. [Arabidopsisthaliana] Length = 120341320234131E-123 >gi|2583123 (AC002387) nucleotide sugar epimerase[Arabidopsis thaliana] Length = 43741420234141E-127 >gb|AAD28780.1|AF134133_1 (AF134133) Lil3 protein [Arabidopsisthaliana] Length = 26241520234153E-94 >gi|2511546 (AF022658) c2h2 zinc finger transcription factor[Arabidopsis thaliana] Length = 2384162023416Tyr_Phospho_Site(724-732)41720234171E-123 >gi|2618723 (U49073) IAA17[Arabidopsis thaliana] >gi|2921756(AF040631) IAA17|AXR3 protein [Arabidopsis thaliana]>gi|4389514|gb|AAB70451 (AC000104) Identical to Arabidopsis gb|AF040632 andgb|U490731AA17/AXR3 gene. ESTs gb|H36782 and gb|F14074 come from thisgene. [Arabidopsis thaliana] Length = 22941820234181E-157 >gi|4138855 (AF098072) IMMUTANS [Arabidopsis thaliana]Length = 3514192023419Tyr_Phospho_Site(1298-1305)42020234203E-41 >gb|AAD45585.1|AF132115_1 (AF132115) cytochrome b-561[Arabidopsis thaliana] Length = 23042120234211E-127 >pir|1525435 chlorophyll a/b-binding protein- Arabidopsisthaliana >gi|16207|emb|0AA395341 (X56062) chlorophyll NB-binding protein[Arabidopsis thaliana] >gi|166644 (M85150) chlorophyll a/b-binding protein[Arabidopsis thaliana] >gi|4678304|emb|0AB41095.1| (AL049655) chlorophyll a/b-binding protein [Arabidopsis thaliana] Length = 24142220234221E-148 >sp|P21216|IPYR_ARATH SOLUBLE INORGANICPYROPHOSPHATASE (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE)>gi|81645|pir||S13379 inorganic pyrophosphatase (EC 3.6.1.1)- Arabidopsisthaliana >gi|16348|emb|CAA40764| (X57545) inorganic pyrophosphatase[Arabidopsis thaliana] Length = 26342320234238E-69 >gi|3928094 (AC005770) zinc finger protein [Arabidopsis thaliana]Length = 27042420234242E-57 >emb|CAA77089| (Y18227) blue copper binding-like protein[Arabidopsis thaliana] Length = 19642520234251E-149 >emb|CAA18252.1| (AL022224) CLV1 receptor kinase like protein[Arabidopsis thaliana] Length = 9924262023426Tyr_Phospho_Site(935-942)42720234271E-157 >gb|AAD18142| (AC006260) plasma membrane intrinsic protein2B [Arabidopsis thaliana] Length = 2854282023428Tyr_Phospho_Site(699-707)42920234291E-125 ) >gb|AAD24640.1|AC00691998 (AC006919) pyruvate kinase[Arabidopsis thaliana] Length = 4644302023430Rgd(1781-1783)43120234311E-134 >gb|AAD24630.1|AC0069198 (AC006919) fructose-bisphosphatealdolase, cytoplasmic [Arabidopsis thaliana] Length = 3584322023432Pkc_Phospho_Site(101-103)43320234331E-136 >gi|3004557 (AC003673) plasma membrane proton pump H+ATPase, PMA1 [Arabidopsis thaliana] Length = 94943420234341E-138 ) >gi|2191128 (AF007269) belongs to the L5P family ofribosomal proteins [Arabidopsis thaliana] Length = 26243520234353E-98 >gi|1946371 (U93215) regulatory protein Viviparous-1 isolog[Arabidopsis thaliana] Length = 78043620234361E-156 >gb|AAD28773.1|AF134126_1 (AF134126) Lhcb3 protein [Arabidopsisthaliana] >gi|5002210|gb|AAD37362.1|AF143691| (AF143691) type III chlorophylla/b binding protein [Arabidopsis thaliana] Length = 26543720234377E-67 >gi|2459430 (AC002332) CUC2 protein [Arabidopsis thaliana]Length 26843820234381E-155 >sp|P04777|CB21_ARATH CHLOROPHYLL A-B BINDING PROTEIN165/180 PRECURSOR (LHCII TYPE I CAB-165/180) (LHCP)>gi|8l 603|pir||A29280 chlorophyll a/b-binding protein ab165- Arabidopsis thaliana>gi|16368|emb|CAA27540| (X03907) chlorophyll a/b binding protein (LHCP AB 65)[Arabidopsis thaliana] >gi|16372|emb|CAA27541| (X03908) chlorophyll a/b bindingprotein (LHCP AB 180) [Arabidopsis thaliana] Length = 26743920234392E-58 >emb|CAA63223| (X92491) TOM20 [Solanum tuberosum] Length =20444020234401E-89 >emb|CAB40742.1| (AJ237751) aquaglyceroporin [Nicotianatabacum] Length = 24744120234411E-29 >gb|AAD15610| (AC006232) selenium-binding protein[Arabidopsis thaliana] Length = 47244220234421E-146 ) >gb|AAD20124| (AC006201) 60S ribosomal protein L2[Arabidopsis thaliana] Length = 25844320234431E-125 >emb|CAB45800.1 (AL080252) nodulin-like protein [Arabidopsisthaliana] Length = 3684442023444Tyr_Phospho_Site(880-887)4452023445Tyr_Phospho_Site(747-754)4462023446Tyr_Phospho_Site(353—361)44720234474E-34 >gi|3421373 (AF079901) 28 kDa cis-Golgi SNARE [Musmusculus] Length = 25044820234481E-64 >sp|Q43794|SYE_TOBAC GLUTAMYL-TRNA SYNTHETASE(GLUTAMATE_TRNA LIGASE) (GLURS) >gi|1084418|pir|S51685 glutamate-tRNA ligase (EC 6.1.1.17) - common tobacco >gi|603867|emb|CAA58506|(X83524) glutamate-tRNA ligase [Nicotiana tabacum] Length = 56944920234491E-110 >emb|CAB16805.1| (Z99708) minor allergen [Arabidopsis thaliana]Length = 27345020234506E-17 >gb|AAD2S848.1|AC007197_1 (AC007197) disease resistance gene, 540 partial [Arabidopsis thaliana] Length = 55445120234511E-65 >emb|CAA74639| (Y14251) glutathione 5-transferase [Arabidopsisthaliana] Length = 20945220234522E-83 >gi|2598932 (AF027157) auxin-responsive protein IAA2[Arabidopsis thaliana] Length = 17445320234538E-56 >gi|3287683 (AC003979) Similar to apoptosis protein MA-3gb|050465 from Mus musculus. [Arabidopsis thaliana] Length = 69345420234541E-125 ) >gi|1764100 (U81805) GDP-D-mannose-4,6-dehydratase[Arabidopsis thaliana] Length = 37345520234551E-109 >gi|3510259 (AC005310) inorganic pyrophosphatase[Arabidopsis thaliana] >gi|3522960|gb|AAC34242.1| (AC004411) inorganicpyrophosphatase [Arabidopsis thaliana] Length = 21645620234562E-20 >emb|CAA07361.1|(AJ006972) TOM1 [Mus musculus] Length =49245720234571E-143 >gb|AAD25595.1|AC007211_17 (AC007211) chlorophyll A/B bindingprotein [Arabidopsis thaliana] >gi|4741946|gb|AAD28770.1|AF1341231(AF134123) Lhcb2 protein [Arabidopsis thaliana] Length = 26545820234581E-79 ) >gb|AAD31350.1|AC0O7212_6 (AC007212) bZIP transcription factor[Arabidopsis thaliana] Length = 1714592023459Pkc_Phospho_Site(2-4)4602023460Pkc_Phospho_Site(9-11)46120234611E-146 >gi|3980396 (AC004561) C-4 sterol methyl oxidase[Arabidopsis thaliana] Length = 2534622023462Tyr_Phospho_Site(620-626)46320234636E-81 ) >gi|3831468 (AC005700) phosphocholine cytidylyltransferase[Arabidopsis thaliana] >gi|5640001|gb|AAD45922.1|AF165912_1 (AF165912)GTP:phosphocholine cytidylyltransferase [Arabidopsis thaliana] Length = 33246420234641E-153 >gi|3850579 (AC005278) Strong similarity to gb|D14550extracellular dermal glycoprotein (EDGP) precursor from Daucus carota. ESTsgb|H37281, gb|T44167, gb|T21813, gb|N38437, gb|Z26470, gb|R65072,gb|N76373, gb|F15470, gb|Z35182, gb|H76373, gb|Z34678 an . . . Length = 43346520234651E-40 >sp|P48724|IF5_PHAVU EUKARYOTIC TRANSLATION INITIATIONFACTOR 5 (EIF-5) >gi|1008881 (L47221) eukaryotic initiation factor 5 [Phaseolusvulgaris] Length = 44346620234662E-96 >sp|P42043|HMZ1_ARATH FERROCHELATASE I,CHLOROPLAST/MITOCHONDRIAL PRECURSOR (PROTOHEME FERRO-LYASE) (HEME SYNTHETASE) >gi|1076325|pir||A54125 ferrochelatase (EC4.99.1.1) precursor, chloroplast- Arabidopsis thaliana >gi|511081|emb|CAA51819|(X73417) ferrochelatase [Arabid4672023467Pkc_Phospho_Site(8-10)46820234681E-132 >dbj|BAA31525| (AB013301) ethylene responsive element bindingfactor [Arabidopsis thaliana] Length = 28146920234691E-112 ) >sp|P28187|ARA4_ARATH RAS-RELATED PROTEIN ARA-4>gi|81633|pir||JS0641 GTP-binding protein ara-4- Arabidopsis thaliana>gi|217839|dbj|BAA00831| (D01026) small GTP-binding protein [Arabidopsisthaliana] >gi|3763922 (AC004450) GTP-binding protein [Arabidopsis thaliana]Length = 2144702023470Rgd(476-478)4712023471Zinc Finger C2h2(514-536)47220234722E-92 >gi|1872521 (U87833) zinc-finger protein Lsd1 [Arabidopsisthaliana] >gi|1872523 (U87834) zinc-finger protein Lsd1 [Arabidopsis thaliana]>gi|5262161|emb|CAB45804.1| (AL080253) zinc-finger protein Lsd1 [Arabidopsisthaliana] Length = 18947320234731E-133 >emb|CAB42872.1| (AJ012423) wall-associated kinase 2[Arabidopsis thaliana] Length = 73247420234742E-30 >gi|2224911 (U93048) somatic embryogenesis receptor-likekinase [Daucus carota] Length = 5534752023475Tyr_Phospho_Site(869-875)47620234763E-46 >dbj|BAA25999| (AB013447) aluminum-induced [Brassica napus]4772023477Rgd(263-265)47820234781E-104 ) >emb|CAA70498| (Y09314) Rab2-like protein [Arabidopsisthaliana] >gi|5281023|emb|CAB45962.1| (Z97343) GTP-binding RAB2A likeprotein [Arabidopsis thaliana] Length = 2114792023479Tyr_Phospho_Site(465-473)4802023480Tyr_Phospho_Site(143-151)48120234812E-36 >emb|CAB39631.1| (AL049481) DNA-directed RNA polymerase[Arabidopsis thaliana] Length = 74848220234828E-28 >dbj|BAA76626.1| (AB019392) muscle specific gene M9 [Homosapiens] >gi|4689150jgb|AAD27784.1|AF077051_| (AF077051) PTD001 [Homosapiens] Length 21848320234831E-148 >gi|3249095 (AC003114) Contains similarity to dihydrofolatereductase (dfr1) gb|L13703 from Schizosaccharomyces pombe. ESTs gb|N37567and gb|T43002 come from this gene. [Arabidopsis thaliana] Length = 55048420234841E-111 >gi|3746809 (AF082882) adenylate kinase [Arabidopsis thaliana]Length = 2464852023485Tyr_Phospho_Site(370-378)48620234867E-61 >gi|549975 (U12858) nucleosome assembly protein I-like protein;similar to mouse nap I, PIR Accession Number JS0707 [Arabidopsis thaliana]Length = 38248720234871E-105 >sp|Q96283IRB1A_ARATH RAS-RELATED PROTEIN RAB11A>gi|2598229|emb|CAA70112| (Y08904) Rab11 protein [Arabidopsis thaliana]>gi|5541 676|emb|CAB51182.1| (AL096859) Rab11 protein [Arabidopsis thaliana]Length = 21748820234884E-89 >gb|AAD25137.1|AC0071273 (AC007127) ubiquitin protein [Arabidopsisthaliana] Length = 5364892023489Zinc_Finger_C2h2(1776-1798)49020234901E-112 >gi|2191174 (AF007270) similar to the peptidase family S16[Arabidopsis thaliana] Length = 109649120234911E-147 >gi|3461837 (AC005315) expansin [Arabidopsis thaliana]>gi|3927842 (AC005727) expansin AtEx6 [Arabidopsis thaliana] Length = 25749220234921E-173 >gi|3157937 (AC002131) Identical to aspartic proteinase cDNAgb|U51036 from A. thaliana. ESTs gb|N96313, gb|T21893, gb|R30158,gb|T21482, gb|T43650, gb|R64749, gb|R65157, gb|T88269, gb|T44552,gb|T22542, gb|T76533, gb|T44350, gb|Z34591, gb|AA728734, gb . . . Length = 50649320234934E-43 >dbj|BAA259891 (089051) ERD6 protein [Arabidopsis thaliana]Length = 4964942023494Tyr_Phospho_Site(419-426)4952023495Tyr_Phospho_Site(1183-1190)49620234961E-162 >emb|CAA71627| (Y10617) 12-oxophytodienoate reductase[Arabidopsis thaliana] Length = 3704972023497Tyr_Phospho_Site(1175-1181)4982023498Pkc_Phospho_Site(18-20)49920234991E-12 >gi|3834382 (AF033109) syntaxin 8 [Rattus norvegicus] Length =23650020235001E-132 >gi|2317729 (AF013627) reversibly glycosylated polypeptide-1[Arabidopsis thaliana] Length = 35750120235019E-93 >sp|P34091|RL6_MESCR 605 RIBOSOMAL PROTEIN L6 (YL16-LIKE)>gi|280374|pir||S28586 ribosomal protein ML16 - common ice plant>gi|19539 |emb|CAA491751 (X69378) ribosomal protein YL16[Mesembryanthemum crystallinum] Length =5022023502Pkc_Phospho_Site(26-28)50320235033E-11>gi|4100433 (AF000378) beta-glucosidase [Glycine max] Length= 2065042023504Tyr_Phospho_Site(1044-1050)5052023505Tyr_Phospho_Site(659-666)50620235064E-66 >gi|12443890 (AC002294) similar to NAM (gp|X92205|1321924)and CUC2 (gp|AB002560|1944132) proteins [Arabidopsis thaliana] Length = 30050720235078E-24 >gi|3608412 (AF079355) protein phosphatase-2c[Mesembryanthemum crystallinum] Length = 3095082023508Tyr_Phospho_Site(392-398)5092023509Tyr_Phospho_Site(184-191)5102023510Tyr_Phospho_Site(877-883)51120235118E-22 >gi|2622711 (AE000918) ferripyochelin binding protein(Methanobacterium thermoautotrophicum] Length = 1515122023512Pkc_Phospho_Site(11-13)51320235132E-20 >ref|NP005998.1|PZNF216| zinc finger protein 216 >gi|3643809(AF062346) zinc finger protein 216 splice variant 1 [Homo sapiens] >gi|3643811(AF062347) zinc finger protein 216 splice variant 2 [Homo sapiens]>gi|3668066|gb|AAC61801.1| (AF062072) zinc finger protein 216 [Homo sapiens]Length = 2135142023514Pkc_Phospho_Site(29-31)51520235151E-103 >sp|Q38912|RAC3_ARATH RAC-LIKE GTP BINDING PROTEINARAC3 >gi|1304413 (U43501) Rac-like protein [Arabidopsis thaliana] >gi|2645643(AF031427) Rho-like GTP binding protein [Arabidopsis thaliana]>gi|2924513|emb|CAA17767.1| (AL022023) Rho1Ps homolog/ Rac-like protein[Arabido51620235164E-46 >emb|CAA72716| (Y11987) FPF1 protein [Sinapis alba] Length =11051720235171E-119 >emb|CAB45987.1| (AL080318) stress-induced protein sti1-likeprotein [Arabidopsis thaliana] Length = 55851820235181E-145 >gi|3980379 (AC004561) cyclin, PCNA [Arabidopsis thaliana]Length = 26451920235191E-66 >emb|CAB16514.1| (Z99281) similar to ADP-ribosylation factor;cDNA EST EMBL:C08179 comes from this gene; cDNA EST EMBL:C08337comes from this gene; cDNA EST EMBL:C09829 comes from this gene; cDNAEST yk291b4.5 comes5202023520Pkc_Phospho_Site(26-28)52120235212E-45 >emb|CAA74401.1| (Y14072) HMG protein [Arabidopsis thaliana]Length = 14452220235224E-40 >pir||562699 photoassimilate-responsive protein PAR-1b precursor- common tobacco >gi|871487|emb|0AA587311 (X83851) mRNA inducible bysucrose and salicylic acid expressed in sugar-accumulating tobacco plants [Ni5232023523Pkc_Phospho_Site(165-167)52420235242E-60 >gi|3600061 (AF080120) contains similarity to DNA bindingproteins [Arabidopsis thaliana] >gi|4850286|emb|CAB43042.1| (AL049876)protein [Arabidopsis thaliana] Length = 31352520235257E-42 >gi|3789911 (AF081802) developmental protein DG1118[Dictyostelium discoideum] Length = 1925262023526Tyr_Phospho_Site(2-8)5272023527Tyr_Phospho_Site(248-254)5282023528Pkc_Phospho_Site(85-87)52920235291E-125 >sp|P28188|ARA5_ARATH RAS-RELATED PROTEIN ARA-5>gi|231 7906 (U89959) ARA-5[Arabidopsis thaliana] Length = 2585302023530Zinc Protease(1367-1376)53120235311E-127) >gb|AAD30573.1|AC007260_4 (AC007260) 50S Ribosomal protein L13[Arabidopsis thaliana] Length 2415322023532Pkc_Phospho_Site(53-55)53320235334E-57 >sp|023760|COMT_CLABR CAFFEIC ACID 3-O-METHYLTRANSFERASE (S-ADENOSYSL-L-METHIONINE:CAFFEIC ACID 3-O-METHYLTRANSFERASE) (COMT) >gi|2240207 (AF006009) caffeic acid O-methyltransferase [Clarkia breweri] Length = 3705342023534Tyr_Phospho_Site(884-892)5352023535Pkc_Phospho_Site(55-57)53620235366E-16 >gi|2281649 (AF003105) AP2 domain containing proteinRAP2.12 [Arabidopsis thaliana] Length = 31753720235376E-34 >emb|CAB39S33.1| (AJ223758) 54 kDa vacuolar H(+)-ATPasesubunit [Sus scrofa] Length = 48353820235383E-19 >ref|NP005998.1|PZNF216| zinc finger protein 216 >gi|3643809(AF062346) zinc finger protein 216 splice variant 1 [Homo sapiens] >gi|13643811(AF062347) zinc finger protein 216 splice variant 2 [Homo sapiens]>gi|3668066|gb|AA061801.1| (AF062072) zinc finger protein 216 [Homo sapiens]Length = 2135392023539Zinc_Finger_C3hc4(1254-1263)5402023S408E-43 >emb|CAB40041.1| (AL049524) alpha NAG [Arabidopsis thaliana]Length = 21254120235413E-64 >emb|CAB53477.1| (AJ245900) CAA30374.1 protein [Oryza sativa]Length = 60354220235421E-93 >pir||S42651 hypothetical protein - rape>gi|16065752|emb|CAB58175.1| (X74225) pod-specific dehydrogenase SAC25[Brassica napus] Length = 32054320235431E-139 >gb|AAD25850.1|AC007197_3 (AC007197) cytochrome p450[Arabidopsis thaliana] Length = 51854420236441E-124 >emb|CAA65988| (X97323) outward rectifying potassium channelKCO1 [Arabidopsis thaliana] >gi|2230761 |emb|CAA69158| (Y07825) kco1[Arabidopsis thaliana] Length = 3635452023545Tyr_Phospho_Site(258-265)54620235469E-38 >emb|CAA74000| (Y13649) homologous to GATA-bindingtranscription factors [Arabidopsis thaliana]>gi|4895246|gb|AA032831.1|AC00765993 (AC007659) GATA-bindingtranscription factor [Arabidopsis thaliana] Le54720235471E-124 >gb|AAD02810| (AF062396) protein phosphatase 2A regulatorysubunit isoform B′ delta [Arabidopsis thaliana] Length = 4775482023548Tyr_Phospho_Site(4-11)54920235491E-32 >db||BAA22813| (026015) CND41, chloroplast nucleoid DNAbinding protein [Nicotiana tabacum] Length = 50255020235501E-105 >gi|3860277 (AC005824) ribosomal protein L10 [Arabidopsisthaliana] >gi|4314394|gb|AAD15604| (AC006232) ribosomal protein L10A[Arabidopsis thaliana] Length = 22255120235515E-42 >gb|AAD43442.1|AF107837| (AF107837) 26S proteasome subunit p40.5[Homo sapiens] Length = 37655220235521E-68 >emb|CAB36757.1| (AL035523) acid phosphatase-like protein[Arabidopsis thaliana] Length = 2605532023553Pkc_Phospho_Site(21-23)55420235540 ) >gi|3482924 (AC003970) Highly similar to cinnamyl alcoholdehydrogenase, gi|1143445[Arabidopsis thaliana] Length = 32255520235554E-94 >gb|AAD50055.1|AC007980_20 (AC007980) ATP-dependentmetalloprotease [Arabidopsis thaliana] Length = 7165562023556Tyr_Phospho_Site(1518-1526)5572023557Tyr_Phospho_Site(254-262)55820235582E-25 >sp|P355591|DE_RAT INSULIN-DEGRADING ENZYME (INSULYSIN)(INSULINASE) (INSULIN PROTEASE) >gi|347022|pir||529509 insulinase (EC3.4.99.45) - rat >gi|56492|emb|CAA47689| (X67269) insulin-degrading enzyme[Rattus norvegic55920235591E-44 >emb|CAA74400.1| (Y14071) HMG protein [Arabidopsis thaliana]>gi 13068715 (AF049236) unknown [Arabidopsis thaliana] Length = 17856020235601E-109 >gi|2281647 (AF003104) AP2 domain containing proteinRAP2.11 [Arabidopsis thaliana] Length = 2555612023561Tyr_Phospho_Site(300-308)5622023562Pkc_Phospho_Site(62-64)56320235639E-61 >emb|CAA71502| (Y10477) chloroplast thylakoidal processingpeptidase [Arabidopsis thaliana] Length = 3405642023564Tyr_Phospho_Site(685-692)56520235651E-12 >gi|3287691 (AC003979) Contains similarity to RING zinc fingerprotein gb|X95455 from Gallus gallus. [Arabidopsis thaliana] Length = 3985662023566Rgd(902-904)5672023567Rgd(1696-1698)56820235684E-41 >gi|2462833 (AF000657) highly similar to froha and frohb,potential frohc, tumor related protein [Arabidopsis thaliana] Length = 6935692023569Pkc_Phospho_Site(8-10)5702023570Tyr_Phospho_Site(1252-1259)57120235713E-22 >gi|4091808 (AF053307) deacetylvindoline 4-O-acetyltransferase[Catharanthus roseus] Length = 43957220235721E-142 >sp|P48422|C86|ARATH CYTOCHROME P450 86A1 (CYPLXXXVI)>gi|940446|emb|CAA62082| (X90458) cytochrome p450 [Arabidopsis thaliana]Length = 51357320235731E-130 ) >gb|AAD50014.1|AC0076519 (AC007651) glutathione transferase[Arabidopsis thaliana] Length = 22057420235744E-24 >gb|AAD33602.1|AF133302_1 (AF133302) type 2 peroxiredoxin [Brassicarapa subsp. pekinensis] Length = 16257520235751E-108 >gi|3860277 (AC005824) ribosomal protein L10 [Arabidopsisthaliana] >gi|4314394|gb|AAD15604| (AC006232) ribosomal protein L10A[Arabidopsis thaliana] Length = 2225762023576Tyr_Phospho_Site(301-308)57720235778E-75 >emb|CAA17547.1| (AL021960) photosystem II oxygen-evolvingcomplex protein 3-like [Arabidopsis thaliana] >gi|3402748|emb|CAA20194.1|(AL031187) photosystem II oxygen-evolving complex protein 3-like [Arabidopsisthaliana] Length = 2235782023578Tyr_Phospho_Site(49-56)57920235791E-83 >emb|CAA18743.1| (AL022604) NAD+ dependent isocitratedehydrogenase subunit 1 [Arabidopsis thaliana] Length = 3675802023580Pkc_Phospho_Site(2-4)58120235815E-40 >pir||552995 arabinogalactan-like protein - loblolly pine >gi|607774(U09556) arabinogalactan-like protein [Pinus taeda] Length = 26458220235824E-23 >emb|CAA10616| (AJ132240) eukaryotic translation initiation factor5 [Zea mays] Length = 45158320235832E-65 >sp|P29545|EF1D_ORYSA ELONGATION FACTOR 1-BETA′ (EF-1-BETA′) >gi|322851|pir||529224 translation elongation factor eEF-1 beta′ chain -rice >gi|218161|dbj|BAA02253| (D12821) elongation factor I beta40 [Oryza sativa]Length = 22358420235841E-36 >gb|AAF00645.1|AC009540_22 (AC009540) cationic amino acidtransporter [Arabidopsis thaliana] Length = 61458520235851E-123 >gi|3152563 (AC002986) Similar to myb-related transcriptionfactors e.g., gb|X98308. EST gb|T22093 and gb|T22697 come from this gene.[Arabidopsis thaliana] Length = 32758620235869E-13 >emb|CAB1022l.1| (Z97336) elicitor like protein [Arabidopsisthaliana] Length = 15858720235871E-100 >gb|AAD35009.1|AF14439_1 (AF144391) thioredoxin-like 5[Arabidopsis thaliana] Length = 1855882023588Rgd(1535-1537)58920235891E-105 >gi|2262173 (AC002329) NADPH thioredoxin reductase[Arabidopsis thaliana] Length = 3835902023590Tyr_Phospho_Site(1491-1497)5912023591Tyr_Phospho_Site(966-972)59220235922E-56 >sp|Q06138|MO25_MOUSE MO25 PROTEIN >gi|2143483|pir||157997hypothetical calcium-binding protein - mouse >gi|262934|bbs|121784 (S51858)Ca2+ binding protein [mice, embryos, Peptide, 341 aa] [Mus sp.] Length = 34159320235934E-99 >gi|3822225 (AF079183) RING-H2 finger protein RHG1a[Arabidopsis thaliana] Length = 19059420235945E-98 ) >dbj|BAA3|144| (AB010916) responce reactor2 [Arabidopsisthaliana] >gi|4678318|emb|CAB41129.1| (AL049658) responce reactor2[Arabidopsis thaliana] Length = 18459520235951E-122 >gi|1046225 (U21952) ethylene response sensor [Arabidopsisthaliana] >gi|2623308 (AC002409) ethylene response sensor (ERS) [Arabidopsisthaliana] >gi|1584365|prf||2122405A ERS gene [Arabidopsis thaliana] Length61359620235963E-28 >gi|2494114 (AC002376) Contains similarity to Daucus glycine-rich cell wall protein (gb|D29974). EST gb|R29840 comes from this gene.[Arabidopsis thaliana] Length = 2125972023597Tyr_Phospho_Site(780-786)59820235982E-80 ) >emb|CAA09|98| (AJ010459) RNA helicase [Arabidopsisthaliana] Length = 14559920235997E-27 >gb|AAD46402.1|AF096246| (AF096246) ethylene-responsivetranscriptional coactivator [Lycopersicon esculentum] Length = 1466002023600Pkc_Phospho_Site(151-153)60120236012E-82 >gb|AAD27618.1|AF124376| (AF124376) 30S ribosomal protein S7[Brassica napus] >gi|5881740|dbj|BAA84431.1| (AP000423) ribosomal protein S7[Arabidopsis thaliana] >gi|5881755|dbj|BAA84446.1| (AP000423) ribosomal proteinS7 [Arabidopsis thaliana] Length = 15560220236022E-79 >gb|AAD|4462| (A0005275) glycosylation enzyme [Arabidopsisthaliana] Length = 44860320236034E-98 >dbj|BAA745281 (AB016471) ARRi protein [Arabidopsis thaliana]Length = 66960420236045E-74 >9113169883 (AF033194) dehydroquinatedehydratase/shikimate:NADP oxidoreductase [Lycopersicon esculentum]>gi|3169888 (AF034411) dehydroquinate dehydratase/shikimate:NADPoxidoreductase [Lycopersicon esculentum] Length = 5456052023605Tyr_Phospho_Site(382-390)6062023606Tyr_Phospho_Site(1085-1092)6072023607Tyr_Phospho_Site(538-545)60820236082E-69 >gb|AAD21 706.| (AC007048) tyrosine transaminase [Arabidopsisthaliana] Length = 4626092023609Tyr_Phospho_Site(216-223)6102023610Pkc_Phospho_Site(10-12)61120236111E-35 >gb|AAD45979.1| (AF115334) MenG [Pseudomonas fluorescens]Length = 16361220236129E-23 >dbj|BAA32422| (AB008107) ethylene responsive element bindingfactor 5 [Arabidopsis thaliana] Length = 30061320236132E-90 >pir||S71219 cytosolic cyclophilin ROC3- Arabidopsis thaliana>gi|1305455 (U40399) cytosolic cyclophilin [Arabidopsis thaliana]>gi 14581104|gb|AAD24594.1|AC0058259 (AC005825) cytosolic cyclophil in(ROC3) [Arabidopsis thaliana] Length = 1736142023614Tyr_Phospho_Site(78-86)6152023615Pkc_Phospho_Site(12-14)6162023616Tyr_Phospho_Site(772-780)61720236171E-106 >emb|CAB45054.1| (AL078637) HSP90-like protein [Arabidopsisthaliana] Length = 62361820236181E-101) >gi|4056469 (AC005990) Strong similarity to gb|M95166 ADP-ribosylation factor from Arabidopsis thaliana. ESTs gb|Z25826, gb|R90191,gb|N65697, gb|AA713150, gb″T46332, gb|AA040967, gb|AA712956, gb|T46403,gb|T46050, gb|A1100391 and gb|Z25043 come from t . . . Length 1886192023619Tyr_Phospho_Site(9-16)62020236203E-44 >gi|3201632 (AC004669) 2A6 protein [Arabidopsis thaliana]Length = 35862120236211E-113 >emb|CAB10222.1| (Z97336) carnitine racemase like protein[Arabidopsis thaliana] Length = 24062220236221E-63 >gi|3341698| (AC003672) blue copper-binding protein II[Arabidopsis thaliana] Length = 20262320236231E-108 >5p|Q96558|UGDH_SOYBN UDP-GLUCOSE 6-DEHYDROGENASE(UDP-GLC DEHYDROGENASE) (UDP-GLCDH) (UDPGDH) >gi|1518540(U5341 8) UDP-glucose dehydrogenase [Glycine max] Length = 4806242023624Tyr_Phospho_Site(515-522)6252023625Tyr_Phospho_Site(1716-1723)62620236262E-16 >emb|CAA84724.1| (Z35663) similar to ribonuleoprotein; cDNA ESTyk222a11.3 comes from this gene; cDNA EST yk222a11.5 comes from this gene;cDNA EST yk432f10.3 comes from this gene; cDNA EST yk432f10.5 comes fromthis gene; cDNA EST yk497a8.3 . . . Length = 30762720236272E-57 >gi|3482933 (AC003970) Similar to cdc2 protein kinases[Arabidopsis thaliana] Length = 9676282023628Tyr_Phospho_Site(4-12)62920236294E-92 >gi|3201969 (AF068332) submergence induced protein 2A [Oryzasativa Length = 19863020236301E-110 >gb|AAD41977.1|AC0064389 (AC006438) unknown protein[Arabidopsis thaliana] Length = 2036312023631Tyr_Phospho_Site(983-990)63220236321E-106 ) >9113482931 (AC003970) germin-like protein [Arabidopsisthaliana] Length = 21963320236334E-68 >gi|4193388 (AF091455) translationally controlled tumor protein[Hevea brasiliensis] Length = 16863420236345E-23 >gi|3193325 (AF069299) contains similarity to pectinesterases[Arabidopsis thaliana] Length = 20963520236352E-45 >emb|CAB52425.1| (AL109770) similar to yeast vacuolar sortingprotein VPS29|PEP11 [Schizosaccharomyces pombe] Length = 18763620236369E-16 >5p|P53173|ERV_YEAST ER-DERIVED VESICLES PROTEIN ERV14>gi|2132531|pir||564058 probable membrane protein YGL054c - yeast(Sacoharomyces cerevisiae) >gi|1322550|emb|CAA96756| (Z72576) ORFYGL054c [Saccharomyces cerevisiae] Length = 13863720236371E-126 >gi|3415113 (AF081201) villin 1 [Arabidopsis thaliana] Length =91063820236381E-125 >pir||558282 dTDP-glucose 4-6-dehydratases homolog -Arabidopsis thaliana >gi|928932|emb|CAA89205| (Z49239) homolog of dTDP-glucose 4-6-dehydratases [Arabidopsis thaliana] >gi Ii 585435|prf||2124427Bdiamide resistance gene [Arabidopsis thaliana] Length = 4456392023639Tyr_Phospho_Site(1102-1110)64020236402E-30 >sp|Q01264|HYUC_PSESN HYDANTOIN UTILIZATION PROTEIN C(ORF4) >gi|151284 (M72717) DL-hydantoinase [Pseudomonas sp.]>gi 121 6833|dbj|BAA01379|(D10494) N-carbamyl-L-amino acid amidohydrolase[Pseudomonas sp.] Length = 4146412023641Tyr_Phospho_Site(127-134)6422023642Tyr_Phospho_Site(407-413)64320236431E-155 >gb|AAD21710.1| (AC007048) protein phosphatase 2C[Arabidopsis thaliana] Length = 29064420236444E-97 >gi|862640 (U20182) MADS-box protein AGL11 [Arabidopsisthaliana] >gi|4538999|emb|CAB39620.1| (AL049481) MADS-box protein AGL11[Arabidopsis thaliana] Length = 23064520236451E-127 >gi|3894171 (AC005312) glutathione s-transferase [Arabidopsisthaliana] Length = 22164620236461E-120 >sp|Q39222|RB1B_ARATH RAS-RELATED PROTEIN RAB11>9112118459|pir||59942 small GTP-binding protein Rabi 1- Arabidopsis thaliana>gi|451860 (L18883) small GTP-binding protein [Arabidopsis thaliana] Length =2166472023647Tyr_Phospho_Site(162-168)64820236487E-29 >dbj|BAA22813| (026015) CND41, chloroplast nucleold DNAbinding protein [Nicotiana tabacum] Length = 50264920236491E-34 >dbj|BAA12797| (085381) cytochrome c oxidase subunit Vbprecursor [Oryza sativa] Length = 1696502023650Pkc_Phospho_Site(60-62)6512023651Tyr_Phospho_Site(927-934)65220236521E-128 >gb|AAD20681| (AC006283) similar to protein Htf9C [Arabidopsisthaliana] Length = 85065320236531E-117 >gb|AAD22643.1|AC0071387 (AC007138) protein transport factor[Arabidopsis thaliana] Length = 8566542023654Tyr_Phospho_Site(951-957)6552023655Pkc_Phospho_Site(31-33)65620236568E-23 >emb|CAB50433.1| (AJ248287) hypothetical DEHYDROGENASE[Pyrococcus abyssi] Length = 33365720236571E-129 >sp|Q08770|RL10_ARATH 60S RIBOSOMAL PROTEIN L10 (WILM'STUMOR SUPPRESSOR PROTEIN HOMOLOG) >gi|478401|pir||JQ2244ribosomal protein L10.e, cytosolic- Arabidopsis thaliana>gi|17682|emb|CAA788561 (Z15157) Wilm's tumor suppressor homologue[Arabidopsis thaliana] Length = 22065820236586E-22 >gb|AAD32844.1 1AC007658_3 (AC007658) thioredoxin-like protein[Arabidopsis thaliana] Length = 13065920236591E-141 >emb|CAB41166.1| (AL049659) cytochrome P450-like protein[Arabidopsis thaliana] Length = 4906602023660Pkc_Phospho_Site(177-179)66120236617E-92 >gi|4056504 (AC005896) zinc finger protein [Arabidopsis thaliana]Length = 1786622023662Tyr_Phospho_Site(441-448)6632023663Tyr_Phospho_Site(1407-1415)66420236642E-60 >gi|1532175 (U63815) similar to protein disulfide isomerase[Arabidopsis thaliana] Length = 13266520236651E-128 >emb|CAB10215.1| (Z97336) ankyrin like protein [Arabidopsisthaliana] Length = 9366662023666Tyr_Phospho_Site(764-772)66720236671E-107 >emb|CAB52747.1| (AJ245629) photosystem I subunit III precursor[Arabidopsis thaliana] Length = 2216682023668Tyr_Phospho_Site(146-152)66920236691E-112 >gi|3065835 (AF058800) methyltransferase [Arabidopsisthaliana] Length = 5046702023670Tyr_Phospho_Site(910-918)6712023671Tyr_Phospho_Site(1058-1064)6722023672Tyr_Phospho_Site(377-383)67320236732E-33 >gi|4097549 (U64907) ATFP4 [Arabidopsis thaliana] Length =17967420236741E-119 >sp|P41916|RAN1_ARATH GTP-BINDING NUCLEAR PROTEINRAN-1 >gi|495729 (L16789) small ras-related protein [Arabidopsis thaliana]>gi|2058278|emb|CAA66047| (X97379) atrani [Arabidopsis thaliana] Length = 22167520236751E-105 >sp|P22953|HS71_ARATH HEAT SHOCK COGNATE 70 KDPROTEIN 1 >gi|1072473|pir||S46302 heat shock cognate protein 70-1 -Arabidopsis thaliana >gi|397482|emb|CAA52684| (X74604) heat shock protein 70cognate [Arabidopsis thaliana] Length = 65167620236762E-89 >gb|AAD39282.1|AC007576_5 (AC007576) Similar to DNA-bindingproteins [Arabidopsis thaliana] Length = 48767720236771E-127 >gi|4056505 (AC005896) nodulin-like protein [Arabidopsisthaliana] Length = 35767820236781E-135 >gi|886116 (U27609) TCH4 protein [Arabidopsis thaliana]>gi|2952473 (AF051338) xyloglucan endotransglycosylase related protein[Arabidopsis thaliana] Length = 28467920236792E-90 >sp|023255|SAHH_ARATH ADENOSYLHOMOCYSTEINASE (8-ADENOSYL-L-HOMOCYSTEINE HYDROLASE) (ADOHCYASE)>gi|2244750|emb|CAB10173.1| (Z97335) adenosylhomocysteinase [Arabidopsisthaliana] >gi|3088579|gb|AAC14714.1| (AF059581) S-adenosyl-L-homocysteinehydrolase [Arabidopsis thaliana] Length = 48568020236809E-23 >dbj|BAA32422| (AB008107) ethylene responsive element bindingfactor 5 [Arabidopsis thaliana] Length = 3006812023681Tyr_Phospho_Site(304-312)6822023682Tyr_Phospho_Site(654-660)68320236832E-58 >sp|Q43434|VATL_GOSHI VACUOLAR ATP SYNTHASE 16 KDPROTEOLIPID SUBUNIT >gi|755148 (U13669) vacuolar H+-ATPase proteolipid(16 kDa) subunit [Gossypium hirsutum] >gi|4519415|dbi|BAA75542.1|(AB024275)vacuolar H+-ATPase c subunit [Citrus unshiu] Length = 16568420236841E-106 >pir||550767 protein kinase - rice >gi|450300 (L27821) proteinkinase [Oryza sativa] Length = 82468520236856E-14 >sp|Q2889|S5A1_MACFA 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 1 (STEROID 5-ALPHA-REDUCTASE 1) (SR TYPE 1)>gi|999036|bbs|164548 (S77162) steroid 5 alpha-reductase type I isoenzyme, SRtype 1 [Cynomolgus monkeys, prostate, Peptide, 263 aa] [Macaca fascicularis]Length = 26368620236861E-131 >gb|AAC34217.1| (AC004411) alcohol dehydrogenase[Arabidopsis thaliana] Length = 2576872023687Tyr_Phospho_Site(146-152)68820236882E-72 >emb|CAB44322.1| (AL078606) phospholipase D-gamma[Arabidopsis thaliana] Length = 86668920236898E-97 >emb|CAB53034.1| (AJ245867) photosystem I subunit XI precursor[Arabidopsis thaliana] Length = 21969020236901E-133 >sp|080585|MTHR_ARATH PROBABLEMETHYLENETETRAHYDROFOLATE REDUCTASE >gi|3212869 (AC004005)unknown protein [Arabidopsis thaliana] Length = 6066912023691Tyr_Phospho_Site(501-508)69220236926E-26 >gb|AAD400|7.1|AF150111_1 (AF150111) small zinc finger-like protein[Arabidopsis thaliana] Length = 9369320236931E-101) >gi|4056469 (AC005990) Strong similarity to gb|M95166 ADP-ribosylation factor from Arabidopsis thaliana. ESTs gb|Z25826, gb|R90191,gb|N65697, gb|AA713150, gb|T46332, gb|AA040967, gb|AA7l 2956, gb|T46403,gb|T46050, gb|A1100391 and gb|Z25043 come from t . . . Length = 1886942023694Zinc Protease(160-169)69520236953E-94 >emb|CAB36847.1| (AL035528) DnaJ-like protein [Arabidopsisthaliana] Length = 1976962023696Tyr_Phospho_Site(1062-1069)69720236971E-83 >sp|P35132|UBC9_ARATH UBIQUITIN-CONJUGATING ENZYME E2-17 KD 9 (UBIQUITIN-PROTEIN LIGASE 9) (UBIQUITIN CARRIER PROTEIN 9)(UBCAT4B) >gi|421857|pir||S32674 ubiquitin-protein ligase (EC 6.3.2.19) UBC9- Arabidopsis thaliana >gi|297884|emb|CAA78714| (Z14990) ubiquitin conjugatingenzyme homolog [Arabidopsis thaliana] >gi|349211 (L00639) ubiquitin conjugatingenzyme [Arabidopsis thaliana] >gi|600391|emb|CAA51201| (X72626) ubiquitinconjugating enzyme E2 [Arabidopsis thaliana] >gi|4455355|emb|CAB36765.1|(AL035524) ubiguitin-protein ligase UBC9 [Arabidopsis thaliana] Length = 14869820236982E-47 >emb|CAA09200| (AJ010461) RNA helicase [Arabidopsis thaliana]Length = 3636992023699Tyr_Phospho_Site(1315-1322)70020237003E-86 >gb|AAD22122.1|AC0062244 (AC006224) isopropylmalate dehydratase[Arabidopsis thaliana] Length = 25670120237019E-11 >pir||559397 probable membrane protein YLR251w - yeast(Saccharomyces cerevisiae) >gi|662333 (U20865) YIr251wp [Saccharomycescerevisiae] Length = 19770220237021E-113 >sp|023755|EF2_BETVU ELONGATION FACTOR 2 (EF-2)>gi|2369714|emb|CAB09900| (Z971 78) elongation factor 2 [Beta vulgaris] Length =84370320237038E-46 >pir||A39634 probable cell cycle control protein cm - fruit fly(Drosophila melanogaster) >gi|2827496|emb|CAA15705.1| (AL009195)EG:30B8.1 [Drosophila melanogaster] Length = 7027042023704Tyr_Phospho_Site(1307-1314)70520237051E-145 >gb|AAD46682.1|AF170910_1 (AF170910) SYNC2 protein [Arabidopsisthaliana] Length = 63870620237061E-65 >gi|3341698 (AC003672) blue copper-binding protein II[Arabidopsis thaliana] Length = 2027072023707Rgd(993-995)7082023708Tyr_Phospho_Site(94-101)7092023709Tyr_Phospho_Site(1050-1057)71020237101E-107 >gb|AAD39612.1|AC007454_11 (AC007454) Similar to gb|X92204 NAMgene product from Petunia hybrida. ESTs gb|H36656 and gb|AA651216 comefrom this gene. [Arabidopsis thaliana] Length = 55771120237117E-88 >gb|AAD27909.1|AC007213_7 (AC007213) receptor protein kinase[Arabidopsis thaliana] Length 85171220237122E-89 >dbj|BAA18577| (090915) peptide chain release factor[Synechocystis sp.] Length = 28871320237134E-54 >gb|AAD21451.1| (AC007017) DNA-binding protein [Arabidopsisthaliana] Length = 1457142023714Tyr_Phospho_Site(7-14)7152023715Tyr_Phospho_Site(467-473)7162023716Tyr_Phospho_Site(185-191)71720237176E-48 >gb|AAD39312.1|AC007258_1 (AC007258) Similar to glutathionetransferase [Arabidopsis thaliana] Length = 23471820237188E-17 >sp|Q42534|PME2_ARATH PECTINESTERASE 2 (PECTINMETHYLESTERASE 2) (PE 2) >gi|2129667|pir||PC4168 pectinesterase (EC3.1.1.11) 2 precursor- Arabidopsis thaliana (fragment) >gi|903894 (U25649)ATPME2 precursor [Arabidopsis thaliana] Length = 5827192023719Tyr_Phospho_Site(1205-1211)7202023720Tyr_Phospho_Site(297-304)72120237211E-103 >sp|Q96252|ATP4_ARATH ATP SYNTHASE DELTA′ CHAIN,MITOCHONDRIAL PRECURSOR >gi|1655484|dbj|BAA136011(088376) delta-prime subunit of mitochondrial F1-ATPase [Arabidopsis thaliana] Length = 20372220237229E-59 >emb|CAB39656.1| (AL049483) nitrogen fixation like protein[Arabidopsis thaliana] Length = 22472320237232E-27 >gi|2984333 (AE000774) Na(+) dependent transporter (Sbffamily) [Aguifex aeolicus] Length = 2977242023724Tyr_Phospho_Site(780-786)72520237252E-45 >gb|AAD22286.1|AC006920 _10 (AC006920) reverse transcriptase[Arabidopsis thaliana] Length = 131172620237264E-44 >emb|CAA63223| (X92491) TOM20 [Solanum tuberosum] Length =20472720237271E-23 >emb|CAB10456.1| (Z97342) nuclear antigen homolog [Arabidopsisthaliana] Length = 35572820237281E-82 >dbj|BAA06384| (030719) ERD15 protein [Arabidopsis thaliana]>gi|3241941 (AC004625) dehydration-induced protein ERD15 [Arabidopsisthaliana] >gi|3894181 (AC005662) ERD15 protein [Arabidopsis thaliana] Length =16372920237296E-24 >gb|AAD24601.1|AC0058258 (AC005825) reverse transcriptase[Arabidopsis thaliana] Length = 131973020237301E-36 >emb|CAB16764.1| (Z99707) heat shock transcription factor HSF4[Arabidopsis thaliana] >gi|3256070|emb|CAA74398| (Y14069) Heat Shock Factor4 [Arabidopsis thaliana] Length = 28473120237311E-68 >gb|AAD25624.1|AC005287_26 (AC005287) Similar to phosphoproteinphosphatase 2A regulatory subunit [Arabidopsis thaliana] Length = 53573220237321E-114 >gb|AAD41426.11AC007727_15 (AC007727) Identical to gb|Y13173Arabidopsis thaliana mRNA for proteasome subunit. EST gb|T76747 comes fromthis gene. Length = 20473320237331E-105) >sp|P41127|RL13_ARATH 60S RIBOSOMAL PROTEIN L13 (BBC1PROTEIN HOMOLOG) >gi|480787|pir||537271 ribosomal protein L13 -Arabidopsis thaliana >gi|404166|emb|CAA53005| (X75162) BBC1 protein[Arabidopsis thaliana] Length = 2067342023734Tyr_Phospho_Site(199-205)73520237354E-41 >emb|CAB44393.1| (AL078610) hydrolase [Streptomyces coelicolor]Length = 26973620237365E-29 >gb|AAD56248.1|AF1862739 (AF186273) leucine-rich repeats containingF-box protein FBL3 [Homo sapiens] Length = 4237372023737Tyr_Phospho_Site(1188-1195)73820237385E-63 >gi|3834306 (AC005679) EST gb|R65024 comes from this gene.[Arabidopsis thaliana] Length = 15673920237391E-78 >gi|1707018 (U78721) CutA isolog [Arabidopsis thaliana] Length =18274020237401E-164 >gb|AAD17364| (AF128396) Arabidopsis thaliana flavin-type blue-light photoreceptor (SW:Q43125) (Pfam: PF00875, Score = 765.2, E = 2.6e-226,N = 1) [Arabidopsis thaliana] Length = 70274120237419E-14 >ref|NP_003913.1|PHERC1| guanine nucleotide exchange factor p532>gi|1477565 (U50078) p532 [Homo sapiens] Length = 486174220237421E-133 >emb|CAA65053| (X95738) proline transporter 2 [Arabidopsisi thaliana] Length = 43974320237436E-93 >gb|AAD39312.1|AC007258_1 (AC007258) Similar to glutathionetransferase [Arabidopsis thaliana] Length = 2347442023744Tyr_Phospho_Site(748-755)74520237451E-120 >gb|AAC24832| (AF061518) manganese superoxide dismutase[Arabidopsis thaliana] Length = 23174620237463E-83 >emb|CAB45986.1| (AL080318) protein [Arabidopsis thaliana]Length = 20674720237473E-22 >gi|895613 (L43505) CASP gene product [Gallus gallus] Length =67574820237484E-39 >gb|AAD21699.1| (AC004793) Contains reverse transcriptasedomain (rvt) PF100078.|[Arabidopsis thaliana] Length = 125374920237491E-124 >emb|CAA19720.1| (AL030978) GH3 like protein [Arabidopsisthaliana] Length = 61275020237501E-69 >emb|CAB36546.1| (AL035440) DNA binding protein [Arabidopsisthaliana] Length = 42775120237513E-75 ) >gi|1707022 (U78721) proline-rich protein isolog [Arabidopsisthaliana] Length = 23975220237521E-122 >gb|AAD17428| (AC006284) methyltransferase [Arabidopsisthaliana] Length = 61975320237533E-15 >gi|2252854 (AF013294) similar to auxin-induced protein[Arabidopsis thaliana] Length = 12275420237541E-101 >gi|2444176 (U94782) unconventional myosin [Helianthusannuus] Length = 12607552023755Tyr_Phospho_Site(661-668)75620237567E-97 >gb|AAD15400| (AC006223) integral membrane protein[Arabidopsis thaliana] Length = 42975720237571E-120 >sp|P42761|GTH3_ARATH GLUTATHIONE S-TRANSFERASEERD13 (GST CLASS PHI) >gi|481822|pir||539542 probable glutathionetransferase (EC 2.5.1.18) (clone ERD13)- Arabidopsis thaliana>gi|497789|db|1BAA04554| (D17673) glutathio75820237581E-114 ) >gi|1707015 (U78721) protein phosphatase 2C isolog[Arabidopsis thaliana] Length = 38075920237591E-108 >gb|AAD24598.1|AC005825_5 (AC005825) chloroplast outer membraneprotein 86, also very similar to GTP-inding protein from pea (GB:L36857)[Arabidopsis thaliana] Length = 120676020237601E-82 >emb|CAA16964| (AL021811) H+-transporting ATP synthasechain9 - like protein [Arabidopsis thaliana] >gi|5730141|emb|CAB52473.1|(AJ245574) ATP synthase beta chain precursor (subunit II) [Arabidopsis thaliana]Length = 21976120237613E-47 >emb|CAA68B48| (Y07563) hin1 [Nicotiana tabacum] Length = 22176220237629E-51 >sp|P28342|GTT1_DIACA GLUTATHIONE S-TRANSFERASE 1 (SR8)(GST CLASS-THETA) >gi|99589|pir||516604 glutathione transferase (EC 2.5.1.18)CARSR8 - clove pink >gi|18330|emb|CAA41279| (X58390) glutathione 5-transferase [Dianthus caryophyllus] >gi|167968 (M64268) glutathione transferase[Dianthus caryophyllus] Length = 2217632023763Tyr_Phospho_Site(192-199)7642023764Tyr_Phospho_Site(1388-1396)76520237651E-38 >emb|CAB40579A| (AJ133639) SAH7 protein [Arabidopsis thaliana]Length = 15976620237664E-17 >ref |NP_003554.1|PSPOP| speckle-type POZ protein>gi|2695708|emb|CAA04199| (AJ000644) SPOP [Homo sapiens] Length = 3747672023767Pkc_Phospho_Site(22-24)76820237683E-31 >sp|P81650|BGAL_PSBAT BETA-GALACTOSIDASE (LACTASE)>gi|4079639|emb|CAA10470| (AJ131635) beta-galactosidase [psychrophilicbacterium TAE 79] Length = 103976920237691E-123 >gi|871782 (L43081) pEARL14 gene product [Arabidopsisthaliana] Length = 76677020237702E-77 >gi|3386612 (AC004665) DNA-binding protein, dbp [Arabidopsisthaliana] Length = 19077120237711E-29 >sp|P42763|DH14_ARATH DEHYDRIN ERD14>gi|556474|dbj|BAA045691 (D17715) ERD14 protein [Arabidopsis thaliana] Length =18577220237728E-13 >emb|CAA88860.1| (Z49068) similar to GTP-binding protein; cDNAEST EMBL:M89111 comes from this gene; cDNA EST EMBL:D27709 comes fromthis gene; cDNA EST EMBL:D27708 comes from this gene; cDNA ESTEMBL:D73788 comes from this gene; cDNA EST yk3 . . . Length = 55677320237731E-107 >gb|AAC34243.1| (AC004411) pto kinase [Arabidopsis thaliana]Length = 36577420237749E-88 >gi|3075394 (AC004484) beta-ketoacyl-CoA synthase[Arabidopsis thaliana] >gi|3559809|emb|CAA09311| (AJ010713) fiddlehead protein[Arabidopsis thaliana] Length = 5507752023775Tyr_Phospho_Site(428-434)77620237761E-125 >emb|CAB45880.1| (AL080282) protein [Arabidopsis thaliana]Length = 139677720237775E-73 >sp|P52810|RS9_PODAN 40S RIBOSOMAL PROTEIN 59 (37)>gi|1321917|emb|CAA65433| (X96613) cytoplasmic ribosomal protein S7[Podospora anserina] Length = 19077820237781E-138 >gi|1066499 (L37606) NADH-dependent glutamate synthase[Medicago sativa] Length = 219477920237794E-37 >gb|AAD19788| (AC006528) zinc-finger protein, 5′ partial[Arabidopsis thaliana] Length = 62678020237801E-10 >gi|3600032 (AF080119) contains similarity to tropomyosin(Pfam: Tropomyosin.hmm, score: 14.57) and ATP synthase (Pfam: ATP-synt B.hmm, score: 10.89) [Arabidopsis thaliana] Length = 46678120237819E-86 >gi|2924779 (AC002334) 3-ketoacyl-CoA thiolase [Arabidopsisthaliana] >gi|2981616|dbj|BAA25248| (AB008854) 3-ketoacyl-CoA thiolase[Arabidopsis thaliana] >gi|2981618|dbi|BAA25249| (AB008855) 3-ketoacyl78220237822E-91 >emb|CAB16762.1| (Z99707) caltractin-like protein [Arabidopsisthaliana] Length = 16778320237833E-50 >gb|AAD21025| (AF106939) 1,4-benzoquinone reductase[Phanerochaete chrysosporium] Length = 2017842023784Tyr_Phospho_Site(1296-1304)7852023785Tyr_Phospho_Site(290-296)78620237862E-52 >gb|AAD22344.1|AC006592_1 (AC006592) anthocyanidin-3-glucosiderhamnosyltransferase, 3′ partial [Arabidopsis thaliana] Length = 4147872023787Tyr_Phospho_Site(49-56)78820237881E-70 ) >emb|CAB41005.1| (AL049640) blue copper-binding protein, 15K(lamin) [Arabidopsis thaliana] Length = 14178920237898E-25 >sp|P73689|SPPA_SYNY3 PROTEASE IV HOMOLOG(ENDOPEPTIDASE IV) >gi|1652816|dbj|BAA177351 (090908) protease IV[Synechocystis sp.] Length = 61079020237901E-120 >sp|Q42599|NUIM_ARATH NADH-UBIQUINONEOXIDOREDUCTASE 23 KD SUBUNIT PRECURSOR (COMPLEX 1-23KD) (Cl-23KD) >9111076356|pir|S52380 NADH dehydrogenase (EC 1.6.99.3)- Arabidopsisthaliana >gi|666977|emb|CAA59061| (X84318) NADH dehydrogenase[Arabidopsis thaliana] >gi|315257379120237914E-91 >gb|AAD44761.1|AF144752_1 (AF144752) 40S ribosomal protein S7homolog [Brassica oleracea] Length = 19179220237921E-121 ) >pir||S36884 ketol-acid reductoisomerase (EC 1.1.1.86) -Arabidopsis thaliana >gi|402552|emb|CAA495O6| (X69880) ketol-acidreductoisomerase [Arabidopsis thaliana] Length = 5917932023793Pkc_Phospho_Site(29-31)79420237948E-53 >gi|4220474 (AC006069) myosin heavy chain [Arabidopsisthaliana] Length = 62979520237951E-140 >sp|O64637|C7C2_ARATH CYTOCHROME P450 76C2 >gi|2979549(AC003680) 7-ethoxycoumarin O-deethylase [Arabidopsis thaliana] Length = 51279620237961E-77 >emb|CAA96435| (Z71753) pectin methylesterase [Nicotianaplumbaginifolia] Length = 31579720237974E-79 >emb|CAB41928.1| (AL049751) short-chain alcohol dehydrogenaselike protein [Arabidopsis thaliana] Length = 26379820237983E-27 >ref|NP006818.1|PTMP21| transmembrane trafficking protein>gi|3915893|sp|P49755|TM21_HUMAN TRANSMEMBRANE PROTEIN TMP21PRECURSOR (S31III125) (S31I125) >gi|1359886|emb|CAA66071| (X97442)transmembrane protein [Homo sapiens] >gi|1407826 (U61734) protein traffickingprotein [Homo sapiens] >gi|3288463|emb|CAA0621 3.1| (AJ004913) integralmembrane protein, Tmp21-I (p23) [Homo sapiens]>gi|4885697|gb|AA031941.1| AC0070556 (AC007055) TM P21 [Homo sapiens]Length = 2197992023799Tyr_Phospho_Site(250-257)80020238008E-19 >gi|3193325 (AF069299) contains similarity to pectinesterases[Arabidopsis thaliana] Length = 2098012023801Tyr_Phospho_Site(236-242)80220238021E-147 >emb|CAB41122.1| (AL049657) proteasome regulatory subunit[Arabidopsis thaliana] Length = 40680320238032E-49 >emb|CAB00039.1| (Z75712) Similarity to S. Pombe BEM1/BUD5suppressor; cDNA EST EMBL:Z14470 comes from this gene; cDNA ESTyk482d4.3 comes from this gene; cDNA EST yk482d4.5 comes from this gene[Caenorhabditis elegans] Length = 40580420238043E-77 >emb|CAB38828.1| (AL035679) proton pump [Arabidopsis thaliana]Length = 8438052023805Pkc_Phospho_Site(74-76)8062023806Pkc_Phospho_Site(147-149)80720238072E-97 >sp|P49177|GBB_ARATH GUANINE NUCLEOTIDE-BINDINGPROTEIN BETA SUBUNIT >gi|557694 (U12232) GTP binding protein betasubunit [Arabidopsis thaliana] >gi|3096915|emb|CAA18825.1| (AL023094) GTPbinding protein beta subunit [A80820238082E-79 >dbj|BAA13947| (D89341) luminal binding protein [Arabidopsisthaliana Length = 66980920238095E-79 >emb|CAA73063.1| (Y12459) cytosolic glutamine synthetaseBrassica napus Length = 35681020238101E-82 >sp|P29525|OLEO_ARATH OLEOSIN >gi|282875|pir||S22538 oleosin- Arabidopsis thaliana >gi|164O5|emb|CAA44225| (X62353) oleosin [Arabidopsisthaliana] >gi|4455257|emb|CAB36756.1| (AL035523) oleosin, 18.5K [Arabidopsisthali81120238111E-108 >gi|4056502 (AC005896) 40S ribosomal protein S5 [Arabidopsisthaliana] Length = 20781220238121E-123 >gi|3319357 (AF077407) contains similarity tophosphoenolpyruvate synthase (ppsA) (GB:AE001056) [Arabidopsis thaliana]Length = 66281320238137E-55 >emb|CAB06417| (Z84377) xylosidase [Aspergillus niger] Length =80481420238143E-11 >gi|3548810 (AC005313) chloroplast nucleoid DNA bindingprotein [Arabidopsis thaliana] Length = 46181520238153E-33 >gi|3402683 (AC004697) patatin-like protein [Arabidopsisthaliana] Length = 49981620238166E-92 >sp|P49209|RL9_ARATH 60S RIBOSOMAL PROTEIN L9>gi|2129720|pir||S71255 ribosomal protein L9- Arabidopsis thaliana>gi|1107489|emb|CAA63024| (X91958) 605 ribosomal protein L9 [Arabidopsisthaliana] Length = 19581720238171E-10 >emb|CAB38212| (AL035601) protein [Arabidopsis thaliana]Length 25281820238181E-130 >gi|2618688 (AC002510) esterase D [Arabidopsis thaliana]Length = 28481920238191E-171 >sp|P46644|AAT3_ARATH ASPARTATE AMINOTRANSFERASE,CHLOROPLAST PRECURSOR (TRANSAMINASE A) >gi|693692 (U15034)aspartate aminotransferase [Arabidopsis thaliana] Length = 44982020238201E-17 >dbj|BAA33206| (AB001888) zinc finger protein [Oryza sativa]Length = 4078212023821Tyr_Phospho_Site(160-167)82220238221E-122 ) >gi|2388578 (AC000098) Similar to Mycobacterium RIpF(gb|Z84395). ESTs gb|T75785, gb|R30580, gb|T04698 come from this gene.[Arabidopsis thaliana] Length = 22382320238231E-129 >gb|AAD25665.1|AC007020_7 (AC007020) ferritin protein [Arabidopsisthaliana] >gi|4588004|gb|AAD25945.1|AF085279_18 (AF085279) hypotheticalferritin subunit [Arabidopsis thaliana] Length = 2598242023824Zinc_Finger_C2h2(360-382)82520238252E-91 >gi|3688799 (AF057137) gamma tonoplast intrinsic protein 2[Arabidopsis thaliana] Length = 2538262023826Tyr_Phospho_Site(60-67)82720238276E-68 >sp|P32110|GTX6_SOYBN PROBABLE GLUTATHIONE S-TRANSFERASE (HEAT SHOCK PROTEIN 26A) (G2-4) >gi|99912|pir||A33654heat shock protein 26A - soybean >gi|169981 (M20363) Gmhsp26-A [Glycinemax] Length = 22582820238281E-101 >gb|AAD39666A|AC007591_31 (AC007591) Is a member of thePF|00903 gyloxalase family. ESTs gb|T44721, gb|T21844 and gb|AA395404 comefrom this gene. [Arabidopsis thaliana] Length = 1748292023829Rgd(1357-1359)83020238305E-90 ) >gb|AAD30232.1|AC007202_14 (AC007202) Is a member of thePF|00171 aldehyde dehydrogenase family. ESTs gb|T21534, gb|N65241 andgb|AA395614 come from this gene. [Arabidopsis thaliana] Length = 50983120238312E-20 >sp|Q46O36|BLC_CITFR OUTER MEMBRANE LIPOPROTEIN BLCPRECURSOR >gi|2121019|pir||40710 outer membrane lipoprotein - Citrobacterfreundii >gi|717136 (U21727) lipocalin precursor [Citrobacter freundii] Length =17783220238322E-89 >sp|P30707|RL9_PEA 60S RIBOSOMAL PROTEIN L9(GIBBERELLIN-REGULATED PROTEIN GA) >gi|100065|pir||S19978 ribosomalprotein L9 - garden pea >gi|20727|emb|CAA46273| (X65155) GA [Pisum sativum]Length = 1938332023833Tyr_Phospho_Site(896-903)83420238342E-87 >sp|P42748|UBC4_ARATH UBIQUITIN-CONJUGATING ENZYME E2-21 KD 1 (UBIQUITIN-PROTEIN LIGASE 4) (UBIQUITIN CARRIER PROTEIN 4)>gi|431266 (L19354) ubiquitin conjugating enzyme [Arabidopsis thaliana] Length= 18783520238359E-83 >gi|1256424 (U51119) cysteine proteinase inhibitor [Brassicacampestris] Length = 20583620238361E-119 >gb|AAD50015.1|AC007651_10 (AC007651) glutathione transferase[Arabidopsis thaliana] Length = 2218372023837Zinc_Finger_C2h2(1242-1265)8382023838Tyr_Phospho_Site(88-96)8392023839Pkc_Phospho_Site(31-33)84020238401E-180 >gi|3355490 (AC004218) dolichyl-phosphate beta-glucosyltransferase [Arabidopsis thaliana] Length 33684120238411E-101 >gi|682728 (L40031) S-adenosyl-L-methionine:trans-caffeoyl-Coenzyme A 3-O-methyltransferase [Arabidopsis thaliana] Length = 21284220238423E-14 >gi|3293547 (AF072709) oxidoreductase [Streptomyces lividans]Length = 31384320238435E-25 >dbj|BAA82843.1| (AB023651) miraculin homologue [Solanummelongena] Length = 16084420238441E-110 >sp|P54888|PSC2_ARATH DELTA 1-PYRROLINE-5-CARBOXYLATESYNTHETASE B (P5CS B) [INCLUDES: GLUTAMATE 5-KINASE (GAMMA-GLUTAMYL KINASE) (GK); GAMMA-GLUTAMYL PHOSPHATE REDUCTASE(GPR) (GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE) (GLUTAMYL-GAMMA-SEMIALDE . . . >gi|887388|emb|CAA60447| (X86778) pyrroline-5-carboxylate synthetase B [Arabidopsis thaliana] >gi|1669658|emb|CAA70527|(Y09355) pyrroline-5-carboxlyate synthetase [Arabidopsis thaliana] Length = 72684520238451E-138 >gi|1020155 (U26936) DNA-binding protein [Arabidopsisthaliana] Length = 23684620238464E-76 >emb|CAB38956.1| (AL049171) pyrophosphate-dependentphosphofructo-1-kinase [Arabidopsis thaliana] Length = 50084720238471E-155 >gi|4185136 (AC005724) trehalose-6-phosphate synthase[Arabidopsis thaliana] Length = 86284820238481E-30 >gi|2642215 (AF030386) NOI protein [Arabidopsis thaliana]Length = 7984920238492E-59 >gi|2739044 (AF024651) polyphosphoinositide binding proteinSsh1p [Glycine max] Length = 32485020238502E-59 >sp|P40602|APG_ARATH ANTER-SPECIFIC PROLINE-RICHPROTEIN APG PRECURSOR >gi|99694|pir||521961 proline-rich protein APG -Arabidopsis thaliana >gi|22599|emb|CAA42925| (X60377) APG [Arabidopsisthaliana] Length = 5348512023851Pkc_Phospho_Site(5-7)85220238521E-104 >gi|3395434 (AC004683) peroxidase [Arabidopsis thaliana]>gi|742248|prf||2009327B peroxidase [Arabidopsis thaliana] Length = 3498532023853Tyr_Phospho_Site(1115-1122)85420238546E-40 >dbj|BAA76393.1| (AB025187) cytochrome c oxidase subunit 6b-1[Oryza sativa] Length = 1698552023855Tyr_Phospho_Site(426-433)85620238566E6E-43 >pir||S52995 arabinogalactan-like protein - loblolly pine >gi|607774(U09556) arabinogalactan-like protein [Pinus taeda] Length = 26485720238573E-91 >sp|P47997|G11A_ORYSA PROTEIN KINASE G11A>gi|100705|pir||B30311 protein kinase C (EC 2.7.1.-) homolog - rice (fragment)>gi|169788 (J04556) G11A protein [Oryza sativa] Length = 53185820238583E-93 ) >gi|3927825 (AC005727) dTDP-glucose 4-6-dehydratase[Arabidopsis thaliana] Length = 34385920238591E-101 >gb|AAD41971.1|AC006438_3 (AC006438) cold acclimation proteinWCOR413 [Triticum aestivum] [Arabidopsis thaliana] Length = 19786020238601E-137 >emb|CAB37533| (AL035538) glycine hydroxymethyltransferaselike protein [Arabidopsis thaliana] Length = 51786120238611E-112 ) >gi|4056502 (AC005896) 405 ribosomal protein S5[Arabidopsis thaliana] Length = 20786220238626E-98 >gi|4204274 (AC004146) ribulose bisphosphate carboxylase,small subunit [Arabidopsis thaliana] Length = 18086320238634E-76 >pir||S71286 oleosin isoform- Arabidopsis thaliana>gi|987014|emb|0AA90877| (Z54164) oleosin [Arabidopsis thaliana]>gi|987016|emb|CAA90878| (Z54165) oleosin [Arabidopsis thaliana] Length = 1918642023864Pkc_Phospho_Site(42-44)8652023865Tyr_Phospho_Site(974-982)8662023866Tyr_Phospho_Site(355-362)86720238676E-35 >dbj|BAA18248| (D90912) ferredoxin [Synechocystis sp.] Length =1228682023868Tyr_Phospho_Site(109-117)8692023869Tyr_Phospho_Site(638-645)87020238705E-30 >emb|CAB55502.1| (AJ131768) tyraminehydroxycinnamoyltransferase [Nicotiana tabacum] Length = 22687120238711E-131 >emb|CAB45850.1| (AL080254) reticuline oxidase-like protein[Arabidopsis thaliana] Length = 53987220238729E-99 ) >emb|CAB41123.1| (AL049657) argininosuccinate synthase-likeprotein [Arabidopsis thaliana] Length = 4988732023873Tyr_Phospho_Site(1364-1370)87420238741E-108 >gb|AAD32833.1|AC00765915 (AC007659) mitochondrial elongationfactor G [Arabidopsis thaliana] Length = 75487520238751E-66 >emb|CAA65533| (X96758) clathrin coat assembly protein AP17[Zea mays] Length = 13287620238763E-92 >sp|Q43117|KPYA_RICCO PYRUVATE KINASE ISOZYME A,CHLOROPLAST PRECURSOR >gi|169703 (M64736) ATP:pyruvatephosphotransferase [Ricinus communis] Length = 58387720238774E-83 >emb|CAB10235.1| (Z97336) auxin-responsive protein IAA1[Arabidopsis thaliana] Length = 16887820238782E-33 >gi|3822225 (AF079183) RING-H2 finger protein RHG1a[Arabidopsis thaliana] Length = 19087920238791E-24 >gb|AAD38289.1|AC00778915 (AC007789) ABA induced plasmamembrane protein [Oryza sativa] Length = 18988020238801E-105 >sp|P10797|RBS3_ARATH RIBULOSE BISPHOSPHATECARBOXYLASE SMALL CHAIN 2B PRECURSOR (RUBISCO SMALL SUBUNIT2B) >gi|68061|pir||RKMUB2 ribulose-bisphosphate carboxylase (EC 4.1.1.39)small chain B2 precursor- Arabidopsis thaliana >gi|16194|emb|CAA32701|(X14564) ribulose bisphosphate carboxylase [Arabidopsis thaliana] Length = 18188120238811E-139 >gi|3402678 (AC004697) adenylate kinase [Arabidopsisthaliana] Length = 2958822023882Tyr_Phospho_Site(98-106)88320238835E-26 >gb|AAD34267.1|AF084419| (AF084419) calmodulin mutantSYNCAM64A [synthetic construct] Length = 14788420238842E-15 >bbs|4807313 kDa-B polypeptide of iron-sulfur protein fractionof NADH:ubiquinone oxidoreductase [cattle, heart, Peptide Mitochondrial Partial,114 aa] Length = 1148852023885Tyr_Phospho_Site(937-944)88620238864E-73 >gb|AAD39281.1|A00075764 (AC007576) initiation factor 5A-4[Arabidopsis thaliana] Length = 1588872023887Pkc_Phospho_Site(69-71)8882023888Tyr_Phospho_Site(100-106)88920238896E-74 >emb|CAB38706.1| (AJ131464) nitrate transporter [Arabidopsisthaliana] Length = 5678902023890Tyr_Phospho_Site(1268-1275)8912023891Zinc_Finger_C2h2(755-775)89220238927E-81 >dbj|BAA24074| (D89824) GTP-binding protein [Arabidopsisthaliana] Length 21089320238932E-33 >gi|4164539 (AF079170) phloem protein [Cucurbita maxima]Length = 15089420238944E-15 >gi|2739366 (AC002505) SF16 like protein [Arabidopsis thaliana]Length = 5168952023895Phospho Site(1301-1307)89620238961E-57 >emb|CAA74052| (Y13724) Transcription factor [Arabidopsisthaliana] Length = 1878972023897Tyr_Phospho_Site(768-775)89820238985E-38 >gi|3599491 (AF085149) aminotransferase [Capsicum chinense]Length = 4598992023899Rgd(210-212)9002023900Tyr_Phospho_Site(1201-1208)90120239011E-144 >pir||S51697 oleoyl-[acyl-carner-protein] hydrolase (EC 3.1.2.14)- Arabidopsis thaliana >gi|2129530|pir||569195 acyl-(acyl carrier protein)thioesterase (clone TE 1-1)- Arabidopsis thaliana >gi|634003|emb|CAA85387|(Z36910) acyl-(acyl carrier protein) thioesterase [Arabidopsis thaliana] Length =41290220239025E-79 >gi|2281629 (AF003095) AP2 domain containing protein RAP2.2[Arabidopsis thaliana] Length 24690320239035E-91 >sp|Q39836|GBLP_SOYBN GUANINE NUCLEOTIDE-BINDINGPROTEIN BETA SUBUNIT-LIKE PROTEIN >gi|1256608|gb|AAB05941.1| (U44850) G beta-like protein [Glycine max] Length = 32590420239047E-87 >gi|1872544 (U89014) early light-induced protein; ELIP[Arabidopsis thaliana] Length = 19590520239051E-108 >gi|507164 (U04818) PITSLRE alpha 2-4 [Homo sapiens]Length = 56290620239061E-121 >gi|3421082 (AF043523) 20S proteasome subunit PAD2[Arabidopsis thaliana] Length 25090720239076E-69 >sp|P55964|KPYG_RICCO PYRUVATE KINASE ISOZYME G,CHLOROPLAST Length = 41890820239081E-108 >gi|3033400 (AC004238) Ser/Thr protein kinase [Arabidopsisthaliana] Length = 125790920239091E-127 >gb|AAD31337.1|AC007354_10 (AC007354) Strong similarity togb|Y09533 involved in starch metabolism from Solanum tuberosum and containsa PF|01326 Pyruvate phosphate dikinase, PEP/pyruvate binding domain. ESTgb|N96757 comes from this gene. [. . . Length = 13589102023910Tyr_Phospho_Site(1347-1355)9112023911Tyr_Phospho_Site(1324-1331)9122023912Rgd(731-733)91320239135E-31 >gb|AAD20708| (AC006300) glucose-induced repressor protein[Arabidopsis thaliana] Length = 6289142023914Tyr_Phospho_Site(4-11)91520239153E-30 >emb|CAB38807.1| (AL035678) nucellin-like protein [Arabidopsisthaliana] Length = 42091620239163E-50 >dbj|BAA22813| (D26015) CND41, chloroplast nucleold DNAbinding protein [Nicotiana tabacum] Length = 50291720239175E-67 >gi|2281633 (AF003097) AP2 domain containing protein RAP2.4[Arabidopsis thaliana] Length = 22991820239182E-98 RBS4 _ARATH RIBULOSE BISPHOSPHATE CARBOXYLASE SMALLCHAIN SUBUNIT9192023919Sugar_Transport_2(364-389)9202023920Tyr_Phospho_Site(218-225)92120239213E-41 >emb|CAB51834.1| (AJ243961) contains eukaryotic protein kinasedomain PF100069 [Oryza sativa] Length = 84492220239224E-28 >9b|AAD28599.1|AF1267429 (AF126742) bundle sheath defective protein2 [Zea mays] Length = 12992320239232E-75 ) >gi|1408473 (U48939) actin depolymerizing factor 2[Arabidopsis thaliana] Length = 13792420239241E-91 >dbj|BAA20084.1| (AB003590) sulfate transporter [Arabidopsisthaliana] >gi|2114106|dbj|BAA20085.1| (AB003591) sulfate transporter[Arabidopsis thaliana] Length 67792520239255E-88 >gi|2317912 (U89959) cathepsin B-like cysteine proteinase[Arabidopsis thaliana] Length = 3579262023926Tyr_Phospho_Site(591-597)92720239271E-110 ) >emb|CAA16940.1| (AL021768) small GTP-binding protein-like[Arabidopsis thaliana] Length = 20092820239281E-112 >gb|AAD28774.1|AF134127_1 (AF134127) Lhcb4.2 protein [Arabidopsisthaliana] Length = 28792920239294E-54 >emb|CAB56149.1| (AJ242970) BTF3b-like factor [Arabidopsisthaliana] Length 16593020239305E-21 >gb|AAD46412.1|AF096262_| (AF096262) ER6 protein [Lycopersiconesculentum] Length = 16893120239311E-105 >sp|P10797|RBS3_ARATH RIBULOSE BISPHOSPHATECARBOXYLASE SMALL CHAIN 2B PRECURSOR (RUBISCO SMALL SUBUNIT2B) >gi|68061|pir||RKMUB2 ribulose-bisphosphate carboxylase (EC 4.1.1.39)small chain B2 precursor- Arabidopsis thaliana >gi|16194|emb|CAA32701|(X14564) ribulose bisphosphate carboxylase [Arabidopsis thaliana] Length = 1819322023932Tyr_Phospho_Site(1153-1159)93320239332E-82 >gi|3834310 (AC005679) Similar to Ubiquitin-conjugating enzymeE2-17 KD gb|D83004 from Homo sapiens. ESTs gb|T88233, gb|Z24464,gb|N37265, gb|H36151, gb|Z34711, gb|AA040983, and gb|T22122 come from thisgene. [Arabidopsis thaliana] Length = 16393420239341E-104 >gb|AAB51571.1| (U75193) germin-like protein [Arabidopsisthaliana] >gi|1755168|gb|AAB5| 573.1| (U75195) germin-like protein [Arabidopsisthaliana] >gi|2239042|emb|CAA73213|(Y12673) GLP3 protein [Arabidopsis thalia9352023935Tyr_Phospho_Site(1372-1379)93620239361E-106 >emb|CAB41927.1| (AL049751) ribosomal protein L13a like protein[Arabidopsis thaliana] Length = 2069372023937Pkc_Phospho_Site(51-53)93820239383E-79 >sp|065788|C7B2_ARATH CYTOCHROME P450 71B2>gi|3164140|dbj|BAA285371 (D78605) cytochrome P450 monooxygenase[Arabidopsis thaliana] Length = 5029392023939Tyr_Phospho_Site(11-18)9402023940Tyr_Phospho_Site(13-20)94120239416E-57 >pir||552578 protein-serine/threonine kinase NPK15 - commontobacco >gi|505146|dbj|BAA06538| (031737) protein-serine/threonine kinase[Nicotiana tabacum] Length = 42294220239428E-94 ) >gi|3337356 (AC004481) protein transport protein SEC61alpha subunit [Arabidopsis thaliana] Length = 47594320239434E-38 >gi|2459440 (AC002332) receptor kinase [Arabidopsis thaliana]Length = 66494420239446E-14 >sp|P80728|MAVI_CUCPE MAVICYANIN >gi|1836088|bbs||79249mavicyanin = 12.752 kda small blue copper-containing stellacyanin-likeglycoprotein/type I cupredoxin [Cucurbita pepo = green zucchini, peelings, Peptide,108 aa] Length = 1089452023945SE-60 >gb|AAD34695.1|AC006341_23 (AC006341) Similar to gb|AJ224359surfeit locus protein 5 (surf5b) from Homo sapiens. [Arabidopsis thaliana] Length= 1509462023946Tyr_Phospho_Site(257-264)94720239471E-78 ) >emb|CAB10195.1| (Z97335) transport protein [Arabidopsisthaliana] Length 76994820239481E-39 >gi|3386612 (AC004665) DNA-binding protein, dbp [Arabidopsisthaliana] Length = 1909492023949Pkc_Phospho_Site(12-14)9502023950Tyr_Phospho_Site(574-580)95120239511E-55 >pir||S37101 ATAF1 protein- Arabidopsis thaliana (fragment)>gi|1345506|emb|CAA52771| (X74755) ATAF1 [Arabidopsis thaliana] Length =2299522023952Pkc_Phospho_Site(45-47)95320239531E-125 >emb|CAB38921.1| (AL035709) bZIP transcription factor-likeprotein [Arabidopsis thaliana] Length = 30595420239541E-93 >emb|CAA72792| (Y12071) thylakoid lumen rotamase [Spinaciaoleracea] Length = 44995520239557E-64 ) >gi|2708746 (AC003952) DnaJ-like chaperonin [Arabidopsisthaliana] Length = 16095620239569E-95 >pir||533612 isocitrate dehydrogenase - soybean Length = 45195720239571E-106 >sp|O23515|RL15_ARATH 605 RIBOSOMAL PROTEIN L15>gi|2245027|emb|CAB10447.1| (Z97341) ribosomal protein [Arabidopsis thaliana]Length = 20495820239581E-63 >gb|AAC28488.1| (AF079588) 1-aminocyclopropane-1-carboxylateoxidase [Sorghum bicolor] Length = 31695920239593E-58 >emb|CAB36546.1| (AL035440) DNA binding protein [Arabidopsisthaliana] Length = 4279602023960Tyr_Phospho_Site(190-196)9612023961Tyr_Phospho_Site(818-825)96220239621E-131 >gi|2511725 (AF021937) catalase 1 [Arabidopsis thaliana]Length = 49296320239631E-19 >gi|1905887 (U92461) recombination factor GdRad54 [Gallusgallus] Length = 73396420239641E-103 >sp|P46283|517P_ARATH SEDOHEPTULOSE-1,7-BISPHOSPHATASE, CHLOROPLAST PRECURSOR (SEDOHEPTULOSE-BISPHOSPHATASE) (SBPASE) (SED(1,7)P2ASE) >gi|1076403|pir||551838sedoheptulose-1,7-biphosphatase-Arabidopsis thaliana >gi|78696520239652E-17 >emb|CAA99819.1| (Z75533) waek similarty with bacillusamyloliquefaciens permease IIBO (Swiss Prot accession number P41029); cDNAEST yk573h3.3 comes from this gene [Caenorhabditis elegans] Length = 37896620239668E-26 >pir||549463 chloroplast RNA binding protein - kidney bean>gi|558629|emb|0AA57551| (X82030) chloroplast RNA binding protein [Phaseolusvulgaris] Length = 28796720239671E-44 >emb|CAA55397| (X78820) casein kinase I [Arabidopsis thaliana]Length = 36496820239681E-105 ) >gb|AAB51565.1| (U75187) germin-like protein [Arabidopsisthaliana] Length = 20496920239692E-96 >emb|CAA65502| (X96727) isocitrate dehydrogenase (NAD+)[Nicotiana tabacum] Length = 3649702023970Pkc_Phospho_Site(26-28)97120239714E-43 >gi|871780 (L43080) pEARLI 1 gene product [Arabidopsisthaliana] >gi|4725947|emb|CAB41718.1| (AL049730) pEARLI 1 [Arabidopsisthaliana] Length = 16897220239722E-16 >sp|P24805|TSJT_TOBAC STEM-SPECIFIC PROTEIN TSJT1>gi|00383|pir||513551 stem-specific protein - common tobacco>gi|20037|emb|CAA36525| (X52283) stem specific, weakly expressed in otherorgans (Nicotiana tabacum] Length = 14997320239731E-18 >gb|AAD210411 (AF116237) pseudouridine synthase 1 [Musmusculus] Length = 3939742023974Tyr_Phospho_Site(95-102)97520239751E-108 ) >prf||1804333B Gln synthetase [Arabidopsis thaliana] Length =43097620239761E-116 >gi|2947070 (AC002521) Ser/Thr protein kinase [Arabidopsisthaliana] Length = 42997720239773E-15 >sp|P74523|YE19_SYNY3 HYPOTHETICAL 17.7 KD PROTEINSLR1419 >gi|1653717|dbj|BAA18628| (D90916) hypothetical protein[Synechocystis sp.] Length = 15997820239787E-20 >gi|3033400 (AC004238) Ser/Thr protein kinase [Arabidopsisthaliana] Length = 12579792023979Tyr_Phospho_Site(28-35)9802023980Pkc_Phospho_Site(16-18)9812023981Rgd(231-233)9822023982Pkc_Phospho_Site(16-18)98320239833E-24 >gi|2854070 (AF044914) histone deacetylase [Arabidopsisthaliana] Length = 30598420239841E-28 >gi|3157924 (AC002131) Contains homology to extensin-likeprotein gb|083227 from Populus nigra. ESTs gb|H76425, gb|T13883, gb|T45348,gb|H37743, gb|AA042634, gb|Z26960 and gb|Z25951 come from this gene. Thereis a similar ORF on the opposite strand. [. . . >gi|4063707 (AF104327) extensin-likeprotein [Arabidopsis thaliana] Length = 1379852023985Receptor_Cytokines_1(1550-1562)98620239861E-113 >gi|3420055 (AC004680) cyclophilin [Arabidopsis thaliana]Length = 20198720239872E-27 >emb|CAB45075.1| (AL078637) serine/threonine kinase-like protein[Arabidopsis thaliana] Length = 4459882023988Zinc_Finger_C2h2(929-950)98920239891E-141 >pir||537495 peroxidase (EC 1.1| .1.7)- Arabidopsis thaliana>gi|405611|emb|CAA50677| (X71794) peroxidase [Arabidopsis thaliana] Length= 3539902023990Tyr_Phospho_Site(1189-1197)99120239915E-92 >sp|P28148|TF22_ARATH TRANSCRIPTION INITIATION FACTORTFIID-2 (TATA-BOX FACTOR 2) (TATA SEQUENCE-BINDING PROTEIN 2)(TBP-2) >gi|99764|pir|S10945 transcription initiation factor IID (clone At-1) -Arabidopsis thaliana >gi|16546|emb|CAA38742| (X54995) transcription initiationfactor II [Arabidopsis thaliana] >gi|4204264 (AC005223) 43453 [Arabidopsisthaliana] >gi|227073|prf||1613452A transcription initiation factor TFIID-1[Arabidopsis thaliana] Length = 20099220239923E-16 >gi|3790581 (AF079179) RING-H2 finger protein RHB1a[Arabidopsis thaliana] Length = 19099320239931E-20 >sp|Q28735|TM21_RABIT TRANSMEMBRANE PROTEIN TMP21PRECURSOR (INTEGRAL MEMBRANE PROTEIN P23)>gi|1370279|emb|CAA66947| (X98303) transmem brane protein [Oryctolaguscuniculus] Length = 2199942023994Tyr_Phospho_Site(112-119)99520239953E-11 >gb|AAD35009.1|AF144391| (AF144391) thioredoxin-like 5 [Arabidopsisthaliana] Length = 1859962023996Tyr_Phospho_Site(1372-1379)99720239977E-12 >sp|P40389|UV22SCHPO UV-INDUCED PROTEIN UV122>gi|629909|pir||S47147 uvi22 protein - fission yeast (Schizosaccharomycespombe) >gi|1076930|pir||JC2442 UV inducible protein, UV122 - fission yeast(Schizosaccharomyces pombe) >gi|499199|emb|CAA84069| (Z34299) uvi22[Schizosaccharomyces pombe] >gi|3184086|emb|CAA19342| (AL023781) uv-induced protein uvi22 [Schizosaccharomyces pombe] Length = 30399820239982E-28 >sp|P3018510H18_ARATH DEHYDRIN RAB18 >gi|282880 pir||S28021rab18 protein- Arabidopsis thaliana >gi|16451|emb|CAA48178| (X68042) RAB18[Arabidopsis thaliana] Length = 18699920239994E-93 >sp|P42795|R111_ARATH 60S RIBOSOMAL PROTEIN LIlA (L16A)>gi|624938|emb|CAA57395| (X81799) ribosomal protein L16 [Arabidopsis thaliana]Length = 182

[0186]

Expressed sequences of arabidopsis thaliana

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