Claims
- 1. Method for increasing yield of an arginine containing protein coded for by a gene, comprising:
- (a) transforming an Escherichia coli host cell with
- (1) a DNA sequence which codes for an arginine tRNA molecule wherein a segment of said DNA sequence
- (i) is complementary to an oligonucleotide having the sequence 5'-TTAGAAG-3', and
- (ii) encodes an anticodon loop of said arginine tRNA molecule, and
- (2) an expression vector comprising said gene coding for a protein, and
- (b) cultivating said Escherichia coli host cell under conditions favoring expression of said protein wherein expression of said protein is enhanced as compared to expression in the absence of said DNA sequence which codes for said arginine tRNA in said Escherichia coli cultivated under identical conditions.
- 2. Method of claim 1, wherein said DNA sequence is incorporated into said expression vector.
- 3. Method of claim 1, wherein said DNA sequence is incorporated into a second vector which is compatible with said expression vector.
- 4. Method of claim 3, wherein said second vector contains said DNA sequence and is plasmid pUBS 100.
- 5. Method of claim 1, wherein said protein is t-PA, a t-PA derivative, urokinase, or a human immunodeficiency virus protein.
- 6. Method of claim 4, wherein said expression vector is plasmid pUBS 98.sl.
- 7. Method of claim 1, wherein said segment of said DNA sequence is complementary to an oligonucleotide having the sequence 5'-GACTTAGAAGGTCGTT-3'.
Priority Claims (1)
Number |
Date |
Country |
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3838377 |
Nov 1988 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 07/435,358, filed Nov. 13, 1989, now abandoned.
Non-Patent Literature Citations (3)
Entry |
Garcia et al., Cell v. 45, 1986, pp. 453-459. |
Winnacker, From Genes to Clones, 1987, VCH, New York, pp. 276-279. |
Pennica et al., Nature, v. 301, 1983, pp. 214-221. |
Continuations (1)
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Number |
Date |
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Parent |
435358 |
Nov 1989 |
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