EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS

Information

• Research Project
• 3500863

• ApplicationId

  3500863

• Core Project Number

  R43HL036496

• Full Project Number

  1R43HL036496-01

• Serial Number

  36496

• FOA Number

• Sub Project Id

• Project Start Date

  4/1/1986 - 38 years ago

• Project End Date

  9/30/1986 - 38 years ago

• Program Officer Name

• Budget Start Date

  4/1/1986 - 38 years ago

• Budget End Date

  9/30/1986 - 38 years ago

• Fiscal Year

  1986

• Support Year

  1

• Suffix

• Award Notice Date

  -

Organizations

• ZymoGenetics, Inc.

A full length coding sequence for human protein C will be cloned. This will be done either by obtaining a 5' end clone from a liver cDNA library or by fusing genomic DNA sequence to our cDNA which lacks amino-terminal coding sequence. Once this clone is complete it will be introduced into an expression vector designed for high level expression in mammalian cells. This construction will be transfected into BHK cells and expressed protein C activity measured. Protein C will be assayed by ELISA and for anticoagulent activity.

IC Name

• Activity

  R43

• Administering IC

  HL

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  839

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  HEM

• Study Section Name

  Hematology Subcommittee 2

• Organization Name

  ZYMOGENETICS, INC.

• Organization Department

• Organization DUNS

• Organization City

  SEATTLE

• Organization State

  WA

• Organization Country

  UNITED STATES

• Organization Zip Code

  98105

• Organization District

  UNITED STATES