Claims
- 1. A method to produce a microbial phytase composition from plant cells, plant parts or plants, comprising the steps of:
- preparing an expression construct which contains a DNA sequence encoding a phytase operably linked to regulatory sequences which direct the expression of the DNA sequence encoding phytase in a plant cell;
- stably transforming a plant cell, plant part or plant with said expression construct;
- culturing said plant cell, plant part or plant under conditions wherein said phytase-encoding DNA is expressed; and
- processing said plant cell, plant part or plant under conditions appropriate for the intended use of the composition.
- 2. The method of claim 1, wherein said expressed microbial phytase catalyzes the liberation of inorganic phosphate from myoinositol hexakis-phosphate.
- 3. The method of claim 2, wherein said step of processing said plant cell, plant part or plant under appropriate conditions comprises the additional step of recovering the microbial phytase from said plant cells, plant parts or plants.
- 4. The method of claim 3, wherein the microbial phytase is fungal.
- 5. The method of claim 4, wherein said fungal phytase is of the genus Aspergillus.
- 6. The method of claim 5, wherein said fungal phytase is of A. ficuum, A. niger, A. awamori or A. nidulans.
- 7. The method of claim 3, wherein said regulatory sequences comprise a constitutive promoter.
- 8. The method of claim 3, wherein said regulatory sequences comprise a tissue-specific promoter.
- 9. The method of claim 3, wherein said regulatory sequences comprise a stage-specific promoter.
- 10. The method of claim 3, wherein said regulatory sequences comprise a signal peptide operably linked to said phytase.
- 11. The expression system of claim 10, wherein said signal peptide is the PR protein PR-S signal peptide from tobacco.
- 12. The method of claim 2, wherein said step of processing said plant cell, plant part or plant under appropriate conditions comprises the additional step of grinding said plant cells, plant parts or plants.
- 13. The method of claim 12, wherein the microbial phytase is fungal.
- 14. The method of claim 13, wherein said fungal phytase is of the genus Aspergillus.
- 15. The method of claim 14, wherein said fungal phytase is of A. ficuum, A. niger, A. awamori or A. nidulans.
- 16. The method of claim 12, wherein said regulatory sequences comprise a constitutive promoter.
- 17. The method of claim 12, wherein said regulatory sequences comprise a tissue-specific promoter.
- 18. The method of claim 12, wherein said regulatory sequences comprise a stage-specific promoter.
- 19. The method of claim 12, wherein said regulatory sequences comprise a signal peptide operably linked to said phytase.
- 20. The expression system of claim 19 wherein said signal peptide is the PR protein PR-S signal peptide from tobacco.
- 21. The method of claim 2, wherein said step of processing said plant cell, plant part or plant under appropriate conditions comprises the additional step of combining said plant cells, plant parts or plants with other components appropriate in a composition for hydrolysis of phytate.
- 22. The method of claim 21, wherein the microbial phytase is fungal.
- 23. The method of claim 22, wherein said fungal phytase is of the genus Aspergillus.
- 24. The method of claim 23, wherein said fungal phytase is of A. ficuum, A. niger, A. awamori or A. nidulans.
- 25. The method of claim 21, wherein said regulatory sequences comprise a constitutive promoter.
- 26. The method of claim 21, wherein said regulatory sequences comprise a tissue-specific promoter.
- 27. The method of claim 21, wherein said regulatory sequences comprise a stage-specific promoter.
- 28. The method of claim 21, wherein said regulatory sequences comprise a signal peptide operably linked to said phytase.
- 29. The expression system of claim 28 wherein said signal peptide is the PR protein PR-S signal peptide from tobacco.
- 30. A composition produced by the method of claim 3, 12 or 21.
Priority Claims (1)
Number |
Date |
Country |
Kind |
91200687 |
Mar 1991 |
EPX |
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Parent Case Info
This application is a divisional of application Ser. No. 08/146,424 filed 2 Nov. 1993, now U.S. Pat. No. 5,5939 which is a continuation-in-part of application Ser. No. 07/756,864 filed 11 Sep. 1991 (abandoned); which is a continuation-in-part of application Ser. No. 07/586,765 filed 21 Sep. 1990 (abandoned).
Divisions (1)
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Number |
Date |
Country |
Parent |
146424 |
Nov 1993 |
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Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
756864 |
Sep 1991 |
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Parent |
586765 |
Sep 1990 |
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