Expression system for altered expression levels

Abstract

A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

Description

FIELD OF THE INVENTION

The present invention relates to the discovery of the lipase regulation cascade of Pseudomonas alcaligenes . Specifically, the present invention provides the nucleic acid and amino acid sequences of various components of the lipase regulation cascade which may be used in expression methods and systems designed for the production of heterologous proteins.

BACKGROUND OF THE INVENTION

The isolation and identification of a microorganism that can naturally secrete a product of potential industrial production is one of, if not the most, vital steps in the process of fermentation biotechnology. The ability to secrete the protein of interest usually leads to easier downstream processing. The next critical stage is the mutagenesis of a naturally occurring strain to a hyper-producing strain. Over a number of years, scientists have developed screening strategies from which a number of exo-protein producing bacteria have been isolated. Following isolation, a large number of rounds of mutagenesis can be used to continuously select higher producing strains. However, classical strain improvement cannot be used indefinitely to further increase production levels. Therefore, a more direct method of characterization and molecular genetic manipulation is needed to achieve higher production levels.

Several patents and publications have claimed or described a lipase modulator gene (WO 94/02617; EP 331,376; Nakanishi et al. (1991) Lipases - Struct. Mech. Genet. Eng. GBF Monographs 16:263-266). However, later research has shown that the product of the gene, now called lif, is concerned with folding of the lipase rather than regulating the expression of the lipase. A review of various lipase expression systems that use the lif gene product can be found in Jaeger et al. (1994) FEMS Microbiol. Rev. 15:29-63.

Another publication discusses the sigma 54 promoter and the types of genes that have been described to be under control of this type of promoter. Morrett and Segovia (1993) J. Bacter. 175:6067-6074.

The search has continued for an expression system that can efficiently express a heterologous protein, particularly a lipase in Pseudomonas , in particular Pseudomonas alcaligenes . Pseudomonas expression of lipase is very difficult and often is at lower levels than industry would like to see.

The present invention solves the problem of low levels of expression of proteins in Pseudomonas as well as other microbial hosts.

SUMMARY OF THE INVENTION

The present invention relates to the discovery of a Pseudomonas lipase regulation cascade and provides individual components of the regulation cascade that can be used in expression systems for the production and secretion of proteins in host cells. The regulation cascade comprises, surprisingly, a two-component part that includes a kinase and a DNA binding regulator. The two components work in concert with a promoter and an upstream binding sequence to efficiently express a protein. The regulation cascade also comprises secretion factors that can be used in host cells to enhance the secretion of produced proteins.

The present invention provides nucleic acid and amino acid sequences for the various components of the Pseudomonas alcaligenes lipase regulation cascade. The present invention also provides new, efficient expression systems, i.e., expression vectors, and host cells that can be used to express proteins at increased levels. The new expression systems allow for increased expression of a protein whose gene is functionally linked to components of the expression system, i.e., components of the lipase regulation cascade. A hyper-producing strain can thus be developed and used in a commercial setting.

In one embodiment of the invention, an isolated nucleic acid encoding a kinase that can regulate the expression of a protein, preferably a lipase, is provided. The nucleic acid encoding a kinase is preferably derived from a Gram-negative bacteria such as a pseudomonad, preferably from Pseudomonas alcaligenes and is most preferably lipQ. Further, nucleic acid encoding the kinase preferably has the sequence as shown in FIGS. 1A-1B (SEQ ID NO: 1) and/or has at least 50% homology with that sequence. The kinase protein is also provided and it is preferably derived from a bacteria, preferably from a Gram-negative bacteria such as a pseudomonad, most preferably, the kinase is from Pseudomonas alcaligenes . In a preferred embodiment, the kinase is LipQ. The kinase preferably has the sequence shown in FIGS. 1A-1B , (SEQ ID NO: 2) and/or has at least 50% homology with that sequence.

In another embodiment, the present invention provides a nucleic acid encoding a kinase that can regulate the expression of a lipase in Pseudomonas alcaligenes . In another embodiment, the present invention provides a kinase capable of regulating the expression of a lipase in Pseudomonas alcaligenes.

In a further embodiment of the invention, an isolated nucleic acid encoding a DNA binding regulator that can regulate the expression of a protein, preferably a lipase, is provided. The DNA binding regulator nucleic acid is preferably lipR. Further, it preferably has the sequence as shown in FIGS. 2A-2B (SEQ ID NO: 3) and/or has at least 50% homology with that sequence. The DNA binding regulator protein is also provided and it is preferably LipR. The DNA binding regulator preferably has the sequence shown in FIGS. 2A-2B (SEQ ID NO: 4) and/or has at least 50% homology with that sequence. Preferably, the DNA binding regulator is from bacteria. More preferably, the DNA binding regulator is from a Gram-negative bacteria such as a pseudomonad. Most preferably, the DNA binding regulator is from Pseudomonas alcaligenes.

In yet a further embodiment, the present invention provides an isolated nucleic acid that encodes a DNA binding regulator that can regulate the expression of a lipase in Pseudomonas alcaligenes . In another embodiment, the present invention provides the DNA binding regulator itself.

In yet another embodiment of the invention, nucleic acid encoding a portion of a polymerase that can regulate the expression of a protein, preferably a lipase, is provided. The polymerase nucleic acid is preferable orfZ. Further, it preferably has the sequence as shown in FIGS. 9A-9B (SEQ ID NO: 36) and/or has at least 75% homology with that sequence. A portion of the polymerase protein is also provided and it is preferable OrfZ. The polymerase protein preferable has the sequence shown in FIGS. 9A-9B (SEQ ID NO: 37) and/or at least 75% homology with the sequence. Preferably, the polymerase is from Gram-negative bacteria such as pseudomonad. Most preferably, the polymerase is from Pseudomonas alcaligenes.

In another embodiment, the kinase, the DNA binding regulator and a portion of the polymerase are present in one nucleic acid. In another embodiment, the kinase, the DNA binding regulator and the polymerase have the nucleic acid sequence shown in FIGS. 4A-4G (SEQ ID NO: 28).

In another embodiment of the invention, an isolated nucleic acid encoding a Pseudomonas alcaligenes sigma 54 promoter is provided.

In a further embodiment of the invention, an isolated nucleic acid encoding a Pseudomonas alcaligenes upstream activating sequence is provided. The upstream activating sequence is preferably UAS. Further, it preferably has the sequence as shown in SEQ ID NO: 5 and/or has at least 50% homology with that sequence. Preferably, the upstream activating sequence is from bacteria. More preferably, the upstream activating sequence is from a Gram-negative bacteria such as a pseudomonad. Most preferably, the upstream activating sequence is from Pseudomonas alcaligenes.

In yet another embodiment of the invention, isolated nucleic acids encoding secretion factors are provided. The secretion factors are preferably XcpP, XcpQ, OrfV, OrfX, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and another protein, OrfY, having the C-terminal amino acid sequence shown in SEQ ID NO: 35. Further, they preferably have the nucleic acid sequence as shown in SEQ ID NOS: 12, 14, 30, 16, 6, 8,10, 18, 20, 22, 24, 26, 32 and 34, respectively, and/or have at least 90% homology with those sequence. The secretion factor proteins are also provided and preferably have the amino acid sequences shown in SEQ ID NOS: 13, 15, 31, 17, 7, 9, 11, 19, 21, 23, 25, 27, 33 and 35, respectively, and/or have at least 90% homology with that sequence. Preferably, the secretion factors are from bacteria. More preferably, the secretion factors are from a Gram-negative bacteria such as a pseudomonad. Most preferably, the secretion factors are from Pseudomonas alcaligenes.

In a further embodiment, the genes encoding the secretion factors XcpP, XcpQ, Orf V, OrfX, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpY, XcpX and OrfY are present in one nucleic acid having the DNA sequence shown in FIGS. 3 AA- 3 BB (SEQ ID NO: 29). Both xcp gene clusters xcpP˜Q and xcpR˜Z are oriented divergently with in between OrfV and OrfX as shown in FIG. 8 .

Another embodiment of the invention includes an isolated nucleic acid encoding a Pseudomonas alcaligenes lux-box binding element and orfV-box binding elements that can regulate expression of a protein.

Yet another embodiment provides nucleic acids that can hybridize to the nucleic acids shown in SEQ ID NOS: 1, 3, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 30, 32, 34 and 36 under high stringency conditions.

In a further embodiment, there is provided an expression system comprising a gene encoding a protein functionally linked to nucleic acids encoding a kinase, a DNA binding regulator, a polymerase, a promoter and an upstream activating sequence. The expression system can also include secretion factors, and their regulatory regions. Preferably, the regulating elements and the secretion factors are from bacteria. More preferably, the regulating elements and the secretion factors are from a Gram-negative bacteria such as a pseudomonad. Most preferably, the regulating elements and the secretion factors are from Pseudomonas alcaligenes.

Another embodiment provides an expression system that can regulate the expression of a lipase in Pseudomonas alcaligenes.

In another embodiment of the invention, replicating plasmids and integrating plasmids containing the expression system or a nucleic acid encoding one or more of the secretion factors are provided.

Also provided are methods of transforming a host cell with a plasmid that contains the expression system and/or a nucleic acid encoding one or more secretion factors as well as transformed host cells containing the expression system and/or a nucleic acid encoding one or more secretion factors. A host cell is transformed by introducing the plasmid to the host cell under appropriate conditions. Preferably, the host cell is electroporated to allow the plasmid to enter the host cell. Preferably, the host cell is bacteria. More preferably, the host cell is a Gram-negative bacteria such as a pseudomonad. Most preferably, the host cell is Pseudomonas alcaligenes.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1B show the DNA (SEQ ID NO: 1) and amino acid sequences (SEQ ID NO: 2) of LipQ from Pseudomonas alcaligenes.

FIGS. 2A-2B show the DNA (SEQ ID NO: 3) and amino acid sequences (SEQ ID NO: 4) of LipR from Pseudomonas alcaligenes.

FIGS. 3A-3B show the DNA sequence (SEQ ID NO: 29) of 17.612 bp from the insert on cosmid #600 containing the secretion factors XcpQ, XcpP, OrfV, OrfX, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and a part of an other protein OrfY from Pseudomonas alcaligenes . The predicted amino acid sequences of the open reading frames (SEQ ID NO: 13, 15, 31, 17, 7, 9, 11, 19, 21, 23, 25, 27, 33 and 35, respectively) are shown in one-letter code below the DNA sequence. Likewise, the terminator sequences are shown as two bolded convergent arrows and the binding elements for regulator, OrfV (orfV-boxes) are shown as a bolded boarded line.

FIGS. 4A-4G show the DNA sequence (SEQ ID NO: 28) of the overlapping 4.377 bp fragment of cosmids #71, #201, #505, #726 that includes the open reading frames of LipQ, Lip R and a part of OrfZ from Pseudomonas alcaligenes . The predicted amino acid sequence of the open reading frames (SEQ ID NO: 2, 4 and 37, respectively) are shown in one-letter code below the DNA sequence. Likewise, the terminator sequence is shown as two bolded convergent arrows, the binding element for auto-inducers (lux-box) and the binding elements for OrfV (orfV-boxes) are shown as a bolded boarded line.

FIG. 5 shows the effect on lipase production of cosmid #505 at 10 liter scale. A threefold higher yield of lipase after fermentation was observed.

FIG. 6 shows production plasmid stability in production strain Ps1084 and Ps1084+cosmid #600 as determined by neomycin resistance.

FIG. 7 shows the theoretical scheme for the action of LipQ, LipR, the sigma 54 promoter and the upstream activating sequence on the DNA strand encoding LipA. The small rectangle on the DNA strand below the D-domain of LipR is the upstream activating sequence (UAS).

FIG. 8 shows the orientation of the xcp-genes from Pseudomonas alcaligenes on the map of cosmid #600 as extracted from SEQ ID NO: 29.

FIGS. 9A-9B shows the DNA (SEQ ID NO: 36) and amino acid sequence (SEQ ID NO: 37) of OrfZ from Pseudomonas alcaligenes.

FIG. 10 shows the proposed model for the regulation cascade of the lipase from Pseudomonas alcaligenes.

DETAILED DESCRIPTION OF THE INVENTION

In order to further improve lipase expression in Pseudomonas alcaligenes , a pragmatic search for limiting factors was initiated. A cosmid library from the wild-type P.alcaligenes genome was used as a donor of DNA fragments to be introduced into a multicopy P. alcaligenes lipase production strain. In total, 485 cosmids were transformed, followed by screening of cosmids containing P.alcaligenes strains with respect to their lipase production activity. Twenty cosmid strains were selected, each of which showed a significant enhancement of lipase expression as judged from various liquid and plate tests. The corresponding cosmids were also tested in a single copy lipase strain and some of them were found to give a threefold increase of lipase expression. The four best cosmids were found to share an overlapping fragment of 5.6 kb. The lipase stimulating activity was localized on a 4.5 kb fragment.

The present invention relates to the identification of a Pseudomonas alcaligenes lipase regulation cascade, which contain multiple components associated with the expression of lipase. As used herein, the term “regulation cascade” relates to the entire complex of individual components identified herein, such as kinase, DNA binding regulator, polymerase, UAS, lux-box, orfv-boxes, secretions factors and their regulatory regions. Components of the regulation cascade can be used alone or in combination with other components to modulate the expression of proteins in host cells. In a preferred embodiment, the host cell is a gram-negative host. In another embodiment, the host cell is a pseudomonad. In another preferred embodiment, the host cell is Pseudomonas alcaligenes.

Preferred desired proteins for expression include enzymes such as esterases; hydrolases including proteases, cellulases, amylases, carbohydrases, and lipases; isomerases such as racemases, epimerases, tautomerases, or mutases; transferases, kinases and phophatases. The proteins may be therapeutically significant, such as growth factors, cytokines, ligands, receptors and inhibitors, as well as vaccines and antibodies. The proteins may be commercially important, such as proteases, carbohydrases such as amylases and glucoamylases, cellulases, oxidases and lipases. The gene encoding the protein of interest may be a naturally occurring gene, a mutated gene or a synthetic gene.

The 4.5 kb fragment was sequenced and found to encode the LipQ, LipR and polymerase proteins (FIGS. 4 A- 4 G). While not intending to be bound by theory, it is believed that these proteins are involved in the regulation of the sigma 54 promoter in front of the lipase (LipA) and lipase modulator (LipB) gene region (see FIG. 7 ). These sigma 54 promoters characteristically have an upstream enhancer region, herein the upstream activating sequence or UAS, which is regulated by proteins. Regulation can be achieved by either a two-component system, such as NtrB-NtrC, or by a one-component system, for example NifA, in which the protein is in close association with the substrate (reviewed by Morett and Segovia, supra).

According to the present invention, expression of a protein can be regulated when a kinase and a DNA binding regulator, which are provided in trans, interact with a promoter and/or an upstream activating sequence which are functionally linked to a gene encoding the protein of interest. Preferably, the expression of the protein is increased.

A “kinase” is an enzyme that can catalyze the transfer of phosphate to either itself or another protein. The kinase of the present invention is preferably LipQ, a kinase that can regulate the expression of a lipase. A LipQ has been isolated from Pseudomonas alcaligenes . As such, the kinase preferably is encoded by a nucleic acid having the DNA sequence shown in FIGS. 1A-1B (SEQ ID NO: 1) and has the amino acid sequence shown in FIGS. 1A-1B (SEQ ID NO: 2). A kinase can act alone or as part of an expression system to regulate the expression of the protein. In some cases, the absence of this kinase will cause the expression of the protein to be decreased or eliminated.

A “DNA binding regulator” is a proteinaceous substance which physically interacts with DNA and, in doing so, influences the expression of genes close to the binding position. The DNA binding regulator is preferably LipR, a DNA binding regulator that can regulate the expression of a lipase. A LipR has been isolated from Pseudomonas alcaligenes. As such, the DNA binding regulator preferably is encoded by a nucleic acid having the DNA sequence shown in FIGS. 2A-2B (SEQ ID NO: 3) and has the amino acid sequence shown in FIGS. 2A-2B (SEQ ID NO: 4). A DNA binding regulator can act alone or as part of an expression system to regulate the expression of the protein. A DNA binding regulator of the present invention can be used alone or in combination with a kinase. The present invention encompasses variants of the DNA binding regulators disclosed herein that are capable of autophosphorylation. Such variants can lead to a constitutively higher expression of the target protein. In some cases, the absence of this DNA binding regulator will cause the expression of the protein to be decreased or eliminated.

As used herein “polymerase” refers to an enzyme that elongates DNA or RNA to obtain larger strands of either DNA or RNA, respectively. It is one of the most crucial factors in the production of proteins, such as lipase. In a preferred embodiment, the polymerase is OrfZ. Thus, in a preferred embodiment, the polymerase preferably is encoded by a nucleic acid having the DNA sequence shown in FIGS. 9A-9B (SEQ ID NO: 36) and has the amino acid sequence shown in FIGS. 9A-9B (SEQ ID NO: 37). The polymerase may play a role in modifying the expression of the desired protein.

Promoters are DNA elements that can promote the expression of a protein. A “sigma 54 promoter” is a bacterial promoter and is a member of a class of sigma factors with a size of approximately 54 Kda. These sigma factors are also known as RpoN proteins. Sigma 54 promoters and their functions are discussed in Morrett and Segovia (1993) J. Bacter. 175:6067-6074. Preferably, the promoter is a Pseudomonas alcaligenes sigma 54 promoter. Most preferably, the sigma 54 promoter is the lipase promoter of P. alcaligenes (SEQ ID NO: 5) (WO 94/02617). According to the present invention, the sigma 54 promoter has an upstream activating sequence.

An “upstream activating sequence” is a binding position for a positively-acting DNA binding regulator. As indicated by its name, the upstream activating sequence is upstream of the transcription start site and is a nucleic acid. The upstream activating sequence is preferably UAS, an upstream activating sequence that can regulate the expression of a lipase, and is preferably derived from Pseudomonas alcaligenes . An upstream activating sequence can act alone or as part of an expression system to regulate the expression of the protein. In some cases, the absence of this upstream activating sequence will cause the expression of the protein to be decreased or eliminated. Preferably, the upstream activating sequence is the consensus: TGT(N) 11 ACA. In the Pseudomonas alcaligenes lipase gene sequence, one specific region around -200 bp from the ATG start codon fits this consensus: TGTtcccctcggtaACA (SEQ ID NO: 5) (WO 94/02617).

A secretion factor is a protein that aids in secreting another protein from a cell. Preferably, the secretion factor is a member of the Xcp protein family and acts in concert with other members of the Xcp protein family. A genomic fragment encoding genes xcpQ, xcpP, orfV, orfX, xcpR, xcpS, xcpT, xcpU, xcpV, xcpW, xcpX, xcpY, xcpZ and the C-terminal part of protein OrfY has been isolated from Pseudomonas alcaligenes . As such, the secretion factors preferably are encoded by a nucleic acid having the DNA sequence shown in FIGS. 3 AA- 3 BB (SEQ ID NO: 29). Specifically and more preferably, the XcpP secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 12 and has the amino acid sequence shown in SEQ ID NO: 13; the XcpQ secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 14 and has the amino acid sequence shown in SEQ ID NO: 15; the OrfV protein is encoded by the DNA sequence shown in SEQ ID NO: 30 and has the amino acid sequence shown in SEQ ID NO: 31; the OrfX protein is encoded by the DNA sequence shown in SEQ ID NO: 16 and has the amino acid sequence shown in SEQ ID NO: 17; the XcpR secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 6 and has the amino acid sequence shown in SEQ ID NO: 7; the XcpS secretion factor is encoded by the DNA sequence shown in SEQ ID NO:8 and has the amino acid sequence shown in SEQ ID NO: 9; the XcpT secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 10 and has the amino acid sequence shown in SEQ ID NO: 11; the XcpU secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 18 and has the amino acid sequence shown in SEQ ID NO: 19; the XcpV secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 20 and has the amino acid sequence shown in SEQ ID NO: 21; the XcpW secretion factor is encoded by the DNA sequence shown in SEQ ID NO: 22 and has the amino acid sequence shown in SEQ ID NO: 23; the XcpX secretion factor is encoded by the DNA sequence shown in SEQ ID NO:24 and has the amino acid sequence SEQ ID NO: 25; the secretion factor XcpY is encoded by the DNA sequence shown in SEQ ID NO: 26 and has the amino acid sequence shown in SEQ ID NO: 27; the secretion factor XcpZ is encoded by the DNA sequence shown in SEQ ID NO: 32 and has the amino acid sequence shown in SEQ ID NO: 33; a part of protein OrfY is encoded by the DNA sequence shown in SEQ ID NO: 34 and has the amino acid sequence shown in SEQ ID NO: 35.

Upstream of the lipQ gene, a promoter region has been identified. Within this promoter region, a lux-box can be recognized, see SEQ ID NO: 28. This lux-box shows significant homology to the binding site for luxR type regulator elements, which are known to be under control of autoinducer (Latifi et al. (1995) Molec. Microb. 17(2):333-323). This lux-box probably represents a linkage between the autoinducer system, LipR and lipase regulation. As such, another embodiment of the invention includes a nucleic acid encoding a lux-box element.

Upstream of the xcpP˜Q, xcpR˜Z gene clusters, the orfX, the orfV genes (SEQ ID NO: 29) and upstream of the orfZ gene (SEQ ID NO: 28) regulatory regions are present. A box can be recognized in the promoter region having the consensus sequence ANAANAANAANAA. These boxes are referred to as orfV-binding elements, because OrfV shows homology with the well-known Escherichia coli regulator MalT. Based upon OrfV homology with the known regulator MalT, OrfV may be a regulator. These orfV-boxes can control the expression of the Xcp-proteins, OrfX as well as OrfV itself. Similarly, the expression of the polymerase OrfZ may be controlled by the orfV-boxes, as shown in FIG. 10 . As such, in an other embodiment, the invention provides a nucleic acid encoding an orfV-box element.

Commonly, when describing proteins and the genes that encode them, the term for the gene is not capitalized and is in italics, i.e., lipQ. The term for the protein is generally in normal letters and the first letter is capitalized, i.e., LipQ.

The kinase, DNA binding regulator, promoter and upstream activating sequence will sometimes be referred to as “the regulating elements” for ease of discussion. The preferred regulating elements are LipQ, LipR, the Pseudomonas alcaligenes polymerase, the Pseudomonas alcaligenes sigma 54 promoter and Pseudomonas alcaligenes UAS, and can regulate the expression of a lipase in Pseudomonas alcaligenes as defined herein. The kinase, the DNA binding regulator and polymerase are proteins, and the promoter and the upstream activating sequence are nucleic acids. In transformed cells, DNA encoding the kinase and DNA binding regulator were multiplied using a plasmid which led in turn to a higher production of the kinase and DNA binding regulator. The increased production of the kinase and DNA binding regulator resulted in higher transcription from the sigma 54 promoter which provides higher expression of the protein of interest.

The kinase and DNA binding regulator of the present invention represent a two-component regulatory system. Preferably, the two components are LipQ and LipR and can regulate the expression of a lipase in Pseudomonas alcaligenes as defined herein. Although other two-component regulatory systems are known, a low degree of homology exists between individual pieces of those systems and the amino acid sequence shown in SEQ ID NOS: 2 and 4.

Embodiments of the invention include a kinase or a DNA binding regulator encoded by a nucleic acid having at least 50% homology with the DNA sequences shown in SEQ ID NOS: 1 or 3, respectively. Preferably, the homology is at least 70%, more preferably at least 90% and most preferably at least 95%.

Also provided are embodiments in which a secretion factor encoded by a nucleic acid having at least 90% homology with the DNA sequence shown in SEQ ID NOS: 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 30, 32, 34. Preferably, the homology is at least 95%, more preferably at least 98%. Homology can be determined by lining up the claimed amino acid or DNA sequence with another sequence and determining how many of the amino acids or nucleotides match up as a percentage of the total. Homology can also be determined using one of the sequence analysis software programs that are commercially available, for example, the TFastA Data Searching Program available in the Sequence Analysis Software Package Version 6.0 (Genetic Computer Group, University of Wisconsin Biotechnology Center, Madison, Wis. 53705).

One can screen for homologous sequences using hybridization as described herein or using PCR with degenerate primers. Chen and Suttle (1995) Biotechniques 18(4):609-610, 612.

Also, in several embodiments of the invention, there are provided nucleic acids that can hybridize with the DNA or fragments thereof, shown in FIGS. 1A-1B , 2 A- 2 B, 3 AA- 3 BB and 9 , SEQ ID NOS: 1, 3, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 30, 32, 34, 36, respectively, under stringent conditions. Stringent hybridization conditions include stringent hybridization and washing conditions as is known to one of ordinary skill in the art. Hybridization and appropriate stringent conditions are described in Sambrook et al. 1989 Molecular Cloning 2d ed., Cold Spring Harbor Laboratory Press, New York.

“Bacteria” include microorganisms of the class Schizomycetes. Bacteria can be either Gram-negative or Gram-positive. Gram-negative bacteria include members of the genera Escherichia, Hemophilus, Klebsiella. Proteus, Pseudomonas, Salmonella, Shigella, Vibrio, Acinetobacter, and Serratia. Gram-positive bacteria include members of the genera Bacillus, Clostridium, Staphylococcus, Streptomyces, Lactobacillus and Lactococcus.

Gram-negative bacteria can be pseudomonads which are strains that are members of the genus Pseudomonas. Examples include Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas glumae, Pseudomonas stutzeri, Pseudomonas fragi, Pseudomonas alcaligenes and Pseudomonas mendocina . A preferred pseudomonad is Pseudomonas alcaligenes. Pseudomonas alcaligenes is also sometimes referred to as Pseudomonas pseudoalcaligenes.

Lipases within the scope of the present invention include those encoded by LipA, which is generally found in close association with a modulating gene known as LipB, LipH, LipX or Lif. Lif from Pseudomonas alcaligenes is the subject of patent application WO 93/02617 as discussed above. LipA genes can be found in a variety of species of bacteria such as Pseudomonas aeruginosa, Pseudomonas stutzeri, Pseudomonas alcaligenes, Pseudomonas cepacia, Pseudomonas glumae, Pseudomonas fragi, Pseudomonas mendocina, Acinetobacter calcaoceticus and Serratia marcescans.

Another embodiment of the invention provides an expression system that can regulate the expression of a protein, preferably a lipase. The expression system includes a kinase, a DNA binding regulator, a polymerase, a sigma 54 promoter and an upstream activating sequence. The expression system can also include secretion factors.

An expression system includes one or more proteins and/or nucleic acids which, when acting together, can increase the expression of a protein in a host cell. The expression system can be encoded on one or more plasmids and may or may not be on the same plasmid as the gene encoding the protein of interest.

The phrase “functionally linked” or “functionally coupled” means that the regulating elements (DNA or protein) interact physically in order to exert their function. This can be a protein/protein, DNA/protein or a DNA/DNA interaction. For example, the DNA binding regulator interacts with the promoter but genes encoding them may be at different sites on the chromosome. As such, the genes encoding the elements can be on different plasmids from each other and from the gene encoding the protein of interest and still work together to regulate expression of the protein.

A plasmid is a nucleic acid molecule which is smaller than the chromosome and can replicate independently of the mechanisms used for chromosomal replication. Typically, a plasmid is a circular DNA molecule. Plasmids can be inserted into host cells where they can replicate and make more copies of the plasmid; hence, replicating plasmid. Some plasmids, called integrating plasmids, can insert the plasmid DNA into the chromosome of the host cell. The plasmid DNA is thus integrated into the chromosome of the host cell. When this happens, the plasmid no longer replicates autonomously but instead replicates in synchrony with the chromosome into which it has been inserted. Thus, whereas a nonintegrated plasmid may be present at several dozen copies per chromosome and replicate independently of the chromosome, the integrated plasmid is present at one copy per chromosome and can replicate only when the chromosome does so.

One embodiment of the invention is directed to a method of transforming a host cell with a plasmid that includes the nucleic acid encoding the expression system. A host cell is a cell into which a plasmid of the present invention can be inserted through, for example, transformation. The host cell is preferably a bacteria. In one embodiment, the host cell is preferably a Gram-negative bacteria. In another preferred embodiment, the host cell is a pseudomonad. Preferably, the host cell is Pseudomonas alcaligenes and the regulating elements of the expression system are from Pseudomonas alcaligenes . The same host cell can be transformed with a further plasmid that includes a nucleic acid that encodes one or more secretion factors. Preferably, the secretion factors are from Pseudomonas alcaligenes.

A transformed host cell is a host cell into which one or more plasmids have been inserted. Transformation can take place by first making the host cell competent to receive the plasmid. The naked DNA is then added directly to the cells and some of the cells take it up and replicate or integrate it. One way of making the cells competent to receive the plasmid is by electroporation as described in the Examples below. Another method that is useful for construction and transferring of cosmid libraries is triparental mating. Kelly-Wintenberg and Montie (1989) J. Bacteriol. 171(11):6357-62.

Lipases produced according to the present invention can be used in a number of applications. Lipases can be used in detergents and other cleaning formulations as well as a number of industrial processes.

EXPERIMENTAL

Materials and Methods

Bacterial Strains

All bacterial strains were propagated with 2xTY as a liquid or solid medium, unless otherwise stated, and are listed in Table 1. For P. alcaligenes strains, the medium was supplemented with the appropriate antibiotics: neomycin (10 mg/l), tetracycline (5 mg/l) and chloramphenicol (3 mg/l); and for transformed Escherichia coli , ampicillin was added at 100 mg/l. For cosmid containing Escherichia coli strains, the medium was supplemented with tetracycline (10 mg/l). P. alcaligenes and E. coli were grown at 37° C., aerobically.

TABLE 1
Bacterial strains used. Tet                                                                        R                                                                    , tetracycline resistant; Neo                                                                        R                                                                    , neomycin
resistant; Cap                                                                        R                                                                    , chloramphenicol resistant; lip, lipase.
        Relevant
Strain  Characteristics
P. alcaligenes:
Ps #1   Cosmid #1
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #26  Cosmid #26
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #27  Cosmid #27
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #57  Cosmid #57
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #71  Cosmid #71
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #91  Cosmid #91
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #131 Cosmid #131
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #201 Cosmid #201
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #344 Cosmid #344
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #371 Cosmid #371
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #399 Cosmid #399
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
PS #401 Cosmid #401
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #404 Cosmid #404
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #490 Cosmid #490
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #505 Cosmid #505
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #540 Cosmid #540
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
PS #597 Cosmid #597
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #600 Cosmid #600
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #638 Cosmid #638
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Ps #726 Cosmid #726
        in Ps 824,
        Tet                                                                        R                                                                    , lip                                                                        −
Lip34   Neo                                                                        R                                                                    , lip                                                                        +
Ps537   lip                                                                        +                                                                     (cured from
        production plasmid
        p24lipo1)
Ps824   lip                                                                        −                                                                    (Lip34 cured from
        production plasmid
        p24lipo1)
Ps 1084 2 copies lipQ-R, lip                                                                        +                                                                    ,
        Neo                                                                        R                                                                    , Cap                                                                        R
Ps93    res                                                                        −                                                                    , mod                                                                        +
Ps1108  Ps93 containing
        inactivation of LipR in
        chromosome
E. coli                                                                     K12:
K802    hsdR                                                                        +                                                                    , hsdM                                                                        +                                                                    , gal                                                                        −                                                                    ,
        mel                                                                        −                                                                    , supE
        Δ(lac-pro AB), galE,
        StrA/Z′, lacl                                                                        q                                                                    , zΔm15,
        proA                                                                        +                                                                    B                                                                        +

TABLE 2
Plasmids used
Plasmid	Relevant Characteristics	Reference
pLAFR3	Cosmid vector derived from	Staskawics et al. 1987
	pLAFR1, Tet R
p24Lipo1	lip + , neoR	equivalent to p24A2δ
		(see WO94/02617)
pUC19	lacZ′, rop −	Yanisch-Perron et al. 1985

Extraction of Extra-Chromosomal DNA

Cosmid and plasmid isolations were performed using the QIAprep Spin Plasmid kit, for 1 ml overnight culture, and the QIAfilter Plasmid Midi Kit, for 100 ml culture isolations (both Qiagen), according to the manufacturers instructions. For Pseudomonas strains, lysozyme (10 μl/ml) was added to the resuspension mix and incubated for 5 minutes at 37° C. to aid cell lysis. Cosmid DNA was eluted from the QIAprep columns with 70° C. milliQ water, as recommended by the manufacturer. For cosmid isolations from 100 ml cultures, strains were grown overnight in Luria Bertani (LB) broth and the elution buffer was heated to 50° C.

Transformation of Pseudomonas alcaligenes

An overnight culture of P. alcaligenes was diluted 1:100 in fresh 2xTY medium (with 10 mg/l neomycin) and the culture incubated at 37° C., in an orbital shaker, until it had reached an OD 550 of 0.6-0.8. Following centrifugation (10 minutes at 4000 rpm), the bacterial pellet was washed twice with a half volume SPM medium (276 mM sucrose; 7 mM NaHPO 4 (pH 7.4); 1 mM MgCl 2 ). The cells were then resuspended in a 1/100 volume SPM medium. Cosmid DNA and 40 μl cells were mixed together and transferred to a 2 mm gap electroporation cuvette (BTX). The cells were electroporated with 1.4 kV, 25 μF, 200Ω, in the Gene Pulser. The electroporation cuvette was washed out with 1 ml 2xTY medium and the cell mixture transferred to a clean 1.5 ml eppendorf. The transformation mixture was then incubated for 45 minutes at 37° C. After incubation, 100 μl was plated onto 2xTY agar supplemented with tetracycline (5 mg/l) or neomycin (10 mg/l) or both (depending on which P. alcaligenes strain is used for electroporation). The transformation of P. alcaligenes cells was carried out at room temperature.

Transformation of Escherichia coli

Transformation of E. coli Wk6 cells were performed using electroporation. Transfer of the cosmids to E. coli K802 cells was performed by infection according to the suppliers instructions (Promega Corporation).

Example 1

Construction of a Cosmid Library From Pseudomonas alcaligenes DNA in E. coli

Chromosomal DNA extracted from P. alcaligenes was fractionated and ligated into cosmid pLAFR3 as described in the Materials and Methods section, above. After ligation, the mixture was transferred into E. coli as described. Tetracycline resistant colonies were isolated and cosmid DNA was prepared from each of them.

Example 2

Transformation of a P. alcaligenes Cosmid Library into P. alcaligenes Overexpressing Lipase

In total, 531 plasmid DNA preparations were isolated from E. coli grown cosmids. With the aid of electroporation (see Methods, above) these were transformed into strain Lip34, a P. alcaligenes strain harboring plasmid p24Lipo1 expressing lipase, resulting in 485 cosmid containing P. alcaligenes strains. For transformation, methods as described were used.

Example 3

Selection of Cosmids Stimulating Lipase Expression

In total, 485 cosmids were transformed, followed by screening of cosmid-containing P. alcaligenes strains with respect to their lipase production activity. Twenty cosmid strains were selected which showed a significant enhancement of lipase expression as judged from various liquid and plate tests (see Table 3). The corresponding cosmids we re also tested in a single copy lipase strain and some of them were found to give a threefold increase in lipase expression. The four best cosmids were found to share an overlapping fragment of 5.6 kb. The lipase stimulating activity was localized on a 4.5 kb fragment of cosmid #71, #201, #505, #726. Sequence analysis of this fragment revealed two open reading frames which showed homology with two component regulatory systems. (see FIGS. 4 AA-G). We have named the genes lipQ, lipR and orfZ. It should be noted that from the four described cosmid-strains, only strains containing cosmids #71, 505 and 726, which has the completed OrfZ , give the highest lipase stimulation in the lactate test (second column in table 3) in comparison to the strain containing cosmid #201.

TABLE 3
	Medium 380 + Soy
Cosmid #	Oil	380 + Lactate	2xTY + hexadecane
1	35.25	19.00	13.00
26	35.25	14.75	9.00
27	26.50	18.25	10.00
57	35.75	9.25	7.50
71	40.25	27.25	16.67
91	22.75	23.00	18.00
131	41.30	11.00	3.00
201	39.00	18.00	10.00
344	32.50	11.00	8.30
371	25.50	13.75	15.00
399	23.00	27.00	9.00
401	26.25	11.75	3.00
404	23.75	21.00	7.00
490	27.00	13.25	16.00
505	63.50	28.75	15.00
540	50.50	17.75	4.25
597	47.00	25.25	25.25
600	32.00	17.00	19.00
638	34.75	8.25	11.00
726	36.75	25.25	21.00
control	20.80	11.50	11.50

Example 4

Evidence for Involvement of LiPQ/LipR in Lipase Expression

In order to assess the role of the lipQ/lipR operon, an insertional inactivation of the LipR ORF was constructed in the chromosome of strain PS93. The resulting mutant, Ps1108 showed a significantly reduced halo on tributyrin agar plates as compared to PS93.

In a second experiment, the lipase expression plasmid, p24lipo1 was introduced into strain Ps1108. The lipase expression was severely impaired as compared to PS93 harboring p24lipo1.

This observation suggests the lipQ/lipR operon as the lipase regulatory proteins.

Example 5

Construction and Characterization of a LipQ/LipR Overexpressing P. alcaligenes Strain

The 4.5 kb EcoRI-HindIII fragment of one of the four lipase stimulating cosmids (#201) was subcloned onto pLAFR3 and inserted into a P. alcaligenes strain with a single lipase gene on the chromosome (Ps537). A threefold higher yield of lipase after a 10 liter fermentation was observed. (See FIG. 5. )

Subsequently, the 4.5 EcoRI-HindIII fragment was inserted onto the lipase expression plasmid p24lipo1. A higher lipase expression was observed as could be concluded from halo size on tributyrin plates. During growth in a shake flask, plasmid instability was observed. In order to overcome this instability, the fragment was also integrated into the chromosome resulting in a strain with 2 lipQ/lipR gene copies into the chromosome (strain Ps1084). Insertion of the lipase expression plasmid p24Lipo1 in this strain resulted in higher lipase expression on the plate, but a plasmid instability during fermentation.

Example 6

Effect of Cosmid #600 on Production Plasmid Stability in Ps1084

Previously, a P. alcaligenes strain had been developed in which a second copy of lipQ-R had been integrated into the chromosome. When a lipase production plasmid (plasmid p24Lipo1) was introduced at high copy number (20) into Ps1084 and the strain fermented (10 liters), plasmid instability was observed. A shake-flask experiment was developed to model the situation in the fermenter. To monitor production plasmid stability and cosmid stability of transformed Ps1084, a week long shake-flask experiment was set up. After overnight growth in 10 ml 2xTY broth (supplemented with the required amount of neomycin and tetracycline), 1 ml of transformed culture was used to inoculate 100 ml fermentation medium 380 plus 200 μl soy oil, in shake-flasks. The inoculated shake flasks were incubated for 24 hours at 37° C. in an orbital shaker. One ml of 24 hour old culture was then used to inoculate successive shake-flasks. Throughout the duration of the experiment, daily samples were taken. The presence of a neomycin marker on the lipase production plasmid was used to monitor plasmid stability. The integrated lipQ-R strain with the high copy lipase production plasmid (Ps1084) was transformed with cosmid #600 to see whether plasmid stability was improved.

FIG. 6 is a graphical representation of production plasmid stability in the transformed and untransformed Ps1084 (in duplicate). After 3-4 days, plasmid instability was detected in Ps1084, observed as the 80% drop in neomycin resistant colonies. Through out the week long experiment, cosmid #600 transformed Psi 084 maintained a high degree of neomycin resistance, suggesting that cosmid #600 stabilized the production plasmid.

Example 7

Characterization of Cosmid #600

Cosmid #600, gave a positive signal when PCR was carried out using xcpR primers based on peptides from xcpR derived from Pseudomonas aeruginosa . The DNA sequence from cosmid #600 was digested with EcoRV and the resulting fragment mixture and purified fragments were ligated with SmaI-digested-pUC19 (Appligene) using the Rapid DNA Ligation kit (Boehringer Mannheim). E. coli cells were then electroporated. Transformants were selected on 2xTY plates containing ampicillin (100 mg/l), X-Gal (Boehringer Mannheim; 40 mg/l) and IPTG (Gibco BRL; 1 mM). Transformants containing the recombinant plasmid were identified as white colonies and single colonies were streaked on to fresh 2xTY agar plates (with ampicillin) for purity.

Sequencing of PCR products, cosmid #600 DNA and subclones of cosmid #600 (see above) was achieved by the Dye deoxy termination method, using the ABI PRISM™ Dye Termination Cycle Sequencing Ready Reaction kit with AmpliTaq® DNA Polymerase, FS (Perkin Elmer) in conjunction with the Applied Biosystems 373A sequencer.

Sequencing of cosmid #600 was initiated with the primers used in the PCR to detect xcpR. In accordance with the restriction map of cosmid #600 (FIG. 8 ), an EcoRV restriction site was identified in the nucleic acid sequence of the PCR product. Sequence analysis revealed that the 609 bp amplification product could be translated to a putative amino acid sequence with 89% homology with P. aeruginosa and 73% with P. putida XcpR protein (amino acid residues 59-262), verifying that the xcpR gene had been identified by PCR.

FIG. 8 show the map of cosmid #600. By doing a PCR reaction with digested DNA, we were able to deduce the location of xcpR on the insert. The position of the xcpR gene suggests that the complete Xcp operon is present in cosmid #600.

To date 17.612 nucleotides, encompassing xcpP, xcpQ, orfV, orfX, xcpR, xcpS, xcpT, xcpU, xcpV, xcpW, xcpX, xcpY, xcpZ and part of protein OrfY have been sequenced (FIGS. 3 AA- 3 BB, SEQ ID NO: 29).

While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth, and as follows in the scope of the appended claims.

All patents and applications discussed in the specification are incorporated herein by reference.

37 1029 base pairs nucleic acid single linear unknown 1ATGGGCGTAT GTTCGCTGGC CAAGGACCAG GAAGTGCTGA TGTGGAACCG CGCCATGGAG 60GAACTCACCG GCATCAGCGC GCAGCAGGTG GTCGGCTCGC GCCTGCTCAG CCTGGAGCAC 120CCCTGGCGCG AGCTGCTGCA GGACTTCATC GCCCAGGACG AGGAGCACCT GCACAAGCAG 180CACCTGCAAC TGGACGGCGA GGTGCGCTGG CTCAACCTGC ACAAGGCGGC CATCGACGAA 240CCGCTGGCGC CGGGCAACAG CGGCCTGGTG CTGCTGGTCG AGGACGTCAC CGAGACCCGC 300GTGCTGGAAG ACCAGCTGGT GCACTCCGAG CGTCTGGCCA GCATCGGCCG CCTGGCCGCC 360GGGGTGGCCC ACGAGATCGG CAATCCGGTC ACCGGCATCG CCTGCCTGGC GCAGAACCTG 420CGCGAGGAGC GCGAGGGCGA CGAGGAGCTC GGCGAGATCA GCAACCAGAT CCTCGACCAG 480ACCAAGCGCA TCTCGCGCAT CGTCCAGTCG CTGATGAACT TCGCCCACGC CGGCCAGCAG 540CAGCGCGCCG AATACCCGGT GAGCCTGGCC GAAGTGGCGC AGGACGCCAT CGGCCTGCTG 600TCGCTGAACC GCCATGGCAC CGAAGTGCAG TTCTACAACC TGTGCGATCC CGAGCACCTG 660GCCAAGGGCG ACCCGCAGCG CCTGGCCCAG GTGCTGATCA ACCTGCTGTC CAACGCCCGC 720GATGCCTCGC CGGCCGGCGG TGCCATCCGC GTGCGTAGCG AGGCCGAGGA GCAGAGCGTG 780GTGCTGATCG TCGAGGACGA GGGCACGGGC ATTCCGCAGG CGATCATGGA CCGCCTGTTC 840GAACCCTTCT TCACCACCAA GGACCCCGGC AAGGGCACCG GTTTGGGGCT CGCGCTGGTC 900TATTCGATCG TGGAAGAGCA TTATGGGCAG ATCACCATCG ACAGCCCGGC CGATCCCGAG 960CACCAGCGCG GAACCCGTTT CCGCGTGACC CTGCCGCGCT ATGTCGAAGC GACGTCCACA 1020GCGACCTGA 1029 342 amino acids amino acid single linear unknown 2Met Gly Val Cys Ser Leu Ala Lys Asp Gln Glu Val Leu Met Trp Asn 1 5 10 15Arg Ala Met Glu Glu Leu Thr Gly Ile Ser Ala Gln Gln Val Val Gly 20 25 30Ser Arg Leu Leu Ser Leu Glu His Pro Trp Arg Glu Leu Leu Gln Asp 35 40 45Phe Ile Ala Gln Asp Glu Glu His Leu His Lys Gln His Leu Gln Leu 50 55 60Asp Gly Glu Val Arg Trp Leu Asn Leu His Lys Ala Ala Ile Asp Glu65 70 75 80Pro Leu Ala Pro Gly Asn Ser Gly Leu Val Leu Leu Val Glu Asp Val 85 90 95Thr Glu Thr Arg Val Leu Glu Asp Gln Leu Val His Ser Glu Arg Leu 100 105 110Ala Ser Ile Gly Arg Leu Ala Ala Gly Val Ala His Glu Ile Gly Asn 115 120 125Pro Val Thr Gly Ile Ala Cys Leu Ala Gln Asn Leu Arg Glu Glu Arg 130 135 140Glu Gly Asp Glu Glu Leu Gly Glu Ile Ser Asn Gln Ile Leu Asp Gln145 150 155 160Thr Lys Arg Ile Ser Arg Ile Val Gln Ser Leu Met Asn Phe Ala His 165 170 175Ala Gly Gln Gln Gln Arg Ala Glu Tyr Pro Val Ser Leu Ala Glu Val 180 185 190Ala Gln Asp Ala Ile Gly Leu Leu Ser Leu Asn Arg His Gly Thr Glu 195 200 205Val Gln Phe Tyr Asn Leu Cys Asp Pro Glu His Leu Ala Lys Gly Asp 210 215 220Pro Gln Arg Leu Ala Gln Val Leu Ile Asn Leu Leu Ser Asn Ala Arg225 230 235 240Asp Ala Ser Pro Ala Gly Gly Ala Ile Arg Val Arg Ser Glu Ala Glu 245 250 255Glu Gln Ser Val Val Leu Ile Val Glu Asp Glu Gly Thr Gly Ile Pro 260 265 270Gln Ala Ile Met Asp Arg Leu Phe Glu Pro Phe Phe Thr Thr Lys Asp 275 280 285Pro Gly Lys Gly Thr Gly Leu Gly Leu Ala Leu Val Tyr Ser Ile Val 290 295 300Glu Glu His Tyr Gly Gln Ile Thr Ile Asp Ser Pro Ala Asp Pro Glu305 310 315 320His Gln Arg Gly Thr Arg Phe Arg Val Thr Leu Pro Arg Tyr Val Glu 325 330 335Ala Thr Ser Thr Ala Thr 340 1416 base pairs nucleic acid single linear unknown 3ATGCCGCATA TCCTCATCGT CGAAGACGAA ACCATCATCC GCTCCGCCCT GCGCCGCCTG 60CTGGAACGCA ACCAGTACCA GGTCAGCGAG GCCGGTTCGG TTCAGGAGGC CCAGGAGCGC 120TACAGCATTC CGACCTTCGA CCTGGTGGTC AGCGACCTGC GCCTGCCCGG CGCCCCCGGC 180ACCGAGCTGA TCAAGCTGGC CGACGGCACC CCGGTACTGA TCATGACCAG CTATGCCAGC 240CTGCGCTCGG CGGTGGACTC GATGAAGATG GGCGCGGTGG ACTACATCGC CAAGCCCTTC 300GATCACGACG AGATGCTCCA GGCCGTGGCG CGTATCCTGC GCGATCACCA GGAGGCCAAG 360CGCAACCCGC CAAGCGAGGC GCCCAGCAAG TCCGCCGGCA AGGGCAACGG CGCCACCGCC 420GAGGGCGAGA TCGGCATCAT CGGCTCCTGC GCCGCCATGC AGGACCTTTA CGGCAAGATC 480CGCAAGGTCG CTCCCACCGA TTCCAACGTA CTGATCCAGG GCGAGTCCGG CACCGGCAAG 540GAGCTGGTCG CGCGTGCGCT GCACAACCTC TCGCGTCGCG CCAAGGCACC GCTGATCTCG 600GTGAACTGCG CGGCCATCCC CGAGACCCTG ATCGAGTCCG AACTGTTCGG CCACGAGAAA 660GGTGCCTTCA CCGGCGCCAG CGCCGGCCGC GCCGGCCTGG TCGAAGCGGC CGACGGCGGC 720ACCCTGTTCC TCGACGAGAT CGGCGAGCTG CCGCTGGAGG CGCAGGCCCG CCTGCTGCGC 780GTGCTGCAGG AGGGCGAGAT CCGTCGGGTC GGCTCGGTGC AGTCACAGAA GGTCGATGTA 840CGCCTGATCG CCGCTACCCA CCGCGACCTC AAGACGCTGG CCAAGACCGG CCAGTTCCGC 900GAGGACCTCT ACTACCGCCT GCACGTCATC GCCCTCAAGC TGCCGCCACT GCGCGAGCGC 960GGCGCCGACG TCAACGAGAT CGCCCGCGCC TTCCTCGTCC GCCAGTGCCA GCGCATGGGC 1020CGCGAGGACC TGCGCTTCGC TCAGGATGCC GAGCAGGCGA TCCGCCACTA CCCCTGGCCG 1080GGCAACGTGC GCGAGCTGGA GAATGCCATC GAGCGCGCGG TGATCCTCTG CGAGGGCGCG 1140GAAATTTCCG CCGAGCTGCT GGGCATCGAC ATCGAGCTGG ACGACCTGGA GGACGGCGAC 1200TTCGGCGAAC AGCCACAGCA GACCGCGGCC AACCACGAAC CGACCGAGGA CCTGTCGCTG 1260GAGGACTACT TCCAGCACTT CGTACTGGAG CACCAGGATC ACATGACCGA GACCGAACTG 1320GCGCGCAAGC TCGGCATCAG CCGCAAGTGC CTGTGGGAGC GCCGTCAGCG CCTGGGCATT 1380CCGCGGCGCA AGTCGGGCGC GGCGACCGGC TCCTGA 1416 471 amino acids amino acid single linear unknown 4Met Pro His Ile Leu Ile Val Glu Asp Glu Thr Ile Ile Arg Ser Ala 1 5 10 15Leu Arg Arg Leu Leu Glu Arg Asn Gln Tyr Gln Val Ser Glu Ala Gly 20 25 30Ser Val Gln Glu Ala Gln Glu Arg Tyr Ser Ile Pro Thr Phe Asp Leu 35 40 45Val Val Ser Asp Leu Arg Leu Pro Gly Ala Pro Gly Thr Glu Leu Ile 50 55 60Lys Leu Ala Asp Gly Thr Pro Val Leu Ile Met Thr Ser Tyr Ala Ser65 70 75 80Leu Arg Ser Ala Val Asp Ser Met Lys Met Gly Ala Val Asp Tyr Ile 85 90 95Ala Lys Pro Phe Asp His Asp Glu Met Leu Gln Ala Val Ala Arg Ile 100 105 110Leu Arg Asp His Gln Glu Ala Lys Arg Asn Pro Pro Ser Glu Ala Pro 115 120 125Ser Lys Ser Ala Gly Lys Gly Asn Gly Ala Thr Ala Glu Gly Glu Ile 130 135 140Gly Ile Ile Gly Ser Cys Ala Ala Met Gln Asp Leu Tyr Gly Lys Ile145 150 155 160Arg Lys Val Ala Pro Thr Asp Ser Asn Val Leu Ile Gln Gly Glu Ser 165 170 175Gly Thr Gly Lys Glu Leu Val Ala Arg Ala Leu His Asn Leu Ser Arg 180 185 190Arg Ala Lys Ala Pro Leu Ile Ser Val Asn Cys Ala Ala Ile Pro Glu 195 200 205Thr Leu Ile Glu Ser Glu Leu Phe Gly His Glu Lys Gly Ala Phe Thr 210 215 220Gly Ala Ser Ala Gly Arg Ala Gly Leu Val Glu Ala Ala Asp Gly Gly225 230 235 240Thr Leu Phe Leu Asp Glu Ile Gly Glu Leu Pro Leu Glu Ala Gln Ala 245 250 255Arg Leu Leu Arg Val Leu Gln Glu Gly Glu Ile Arg Arg Val Gly Ser 260 265 270Val Gln Ser Gln Lys Val Asp Val Arg Leu Ile Ala Ala Thr His Arg 275 280 285Asp Leu Lys Thr Leu Ala Lys Thr Gly Gln Phe Arg Glu Asp Leu Tyr 290 295 300Tyr Arg Leu His Val Ile Ala Leu Lys Leu Pro Pro Leu Arg Glu Arg305 310 315 320Gly Ala Asp Val Asn Glu Ile Ala Arg Ala Phe Leu Val Arg Gln Cys 325 330 335Gln Arg Met Gly Arg Glu Asp Leu Arg Phe Ala Gln Asp Ala Glu Gln 340 345 350Ala Ile Arg His Tyr Pro Trp Pro Gly Asn Val Arg Glu Leu Glu Asn 355 360 365Ala Ile Glu Arg Ala Val Ile Leu Cys Glu Gly Ala Glu Ile Ser Ala 370 375 380Glu Leu Leu Gly Ile Asp Ile Glu Leu Asp Asp Leu Glu Asp Gly Asp385 390 395 400Phe Gly Glu Gln Pro Gln Gln Thr Ala Ala Asn His Glu Pro Thr Glu 405 410 415Asp Leu Ser Leu Glu Asp Tyr Phe Gln His Phe Val Leu Glu His Gln 420 425 430Asp His Met Thr Glu Thr Glu Leu Ala Arg Lys Leu Gly Ile Ser Arg 435 440 445Lys Cys Leu Trp Glu Arg Arg Gln Arg Leu Gly Ile Pro Arg Arg Lys 450 455 460Ser Gly Ala Ala Thr Gly Ser465 470 19 base pairs nucleic acid single linear unknown 5GCCTGGAGGA TTACCAGTC 19 1512 base pairs nucleic acid single linear unknown 6ATGTCCACCG ATACCCACGC CGCCCTGACG GCTCCCGCAA GCCCCGCCTT GCGCCCGCTG 60CCCTTCGCCT TCGCCAAACG CCACGGCGTG CTGCTGCGCG AGCCCTTCGG CCAGGTCCAG 120CTGCAGGTGC GCCGCGGTGC CAGCCTGGCC GCCGTGCAGG AGGCCCAGCG CTTCGCCGGC 180CGCGTGCTGC CGCTGCACTG GCTGGAGCCC GAGGCCTTCG AGCAGGAGCT GGCCCTGGCC 240TACCAGCGCG ACTCCTCCGA GGTGCGGCAG ATGGCCGAGG GCATGGGTGC CGAACTTGAC 300CTAGCCAGCC TGGCCGAACT CACTCCCGAA TCCGGCGACC TGCTGGAGCA GGAAGATGAC 360GCGCCGATCA TCCGCCTGAT CAACGCCATC CTCAGCGAGG CGATCAAGGC CGGCGCCTCC 420GACATCCACC TGGAAACCTT CGAGAAACGC CTGGTGGTGC GCTTTCGCGT CGACGGCATC 480CTCCGCGAAG TGATCGAACC GCGCCGCGAG CTGGCGGCGC TGCTGGTCTC GCGGGTCAAG 540GTCATGGCGC GCCTGGACAT CGCCGAGAAG CGCGTACCGC AGGACGGCCG TATTTCGCTC 600AAGGTCGGCG GTCGCGAGGT GGATATCCGC GTCTCCACCC TGCCGTCGGC CAACGGCGAG 660CGGGTGGTGC TGCGTCTGCT CGACAAGCAG GCCGGGCGCC TGTCGCTCAC GCATCTGGGC 720ATGAGCGAGC GCGACCGCCG CCTGCTCGAC GACAACCTGC GCAAGCCGCA CGGCATCATC 780CTAGTCACCG GCCCCACCGG CTCGGGCAAG ACCACCACCC TGTACGCCGG CCTGGTCACC 840CTCAACGACC GCTCGCGCAA TATCCTCACG GTGGAAGACC CGATCGAGTA CTACCTGGAA 900GGCATCGGCC AGACCCAGGT CAACCCGCGG GTGGACATGA CCTTCGCCCG CGGCCTGCGC 960GCCATCCTGC GCCAGGACCC GGACGTGGTG ATGGTCGGCG AGATCCGCGA CCAGGAGACC 1020GCCGACATCG CCGTGCAGGC CTCGCTCACC GGCCACCTGG TGCTCTCCAC CCTGCACACC 1080AACAGCGCCG TCGGCGCCGT CACCCGCCTG GTCGACATGG GCGTCGAGCC CTTCCTGCTG 1140TCGTCGTCCC TGCTCGGCGT GCTGGCCCAG CGCCTGGTGC GCGTGCTCTG CGTGCACTGC 1200CGCGAGGCGC GCCCGGCTGA CGCGGCCGAG TGCGGCCTGC TCGGCCTCGA CCCGCACAGC 1260CAGCCCCTGA TCTACCACGC CAAGGGCTGC CCGGAGTGCC ACCAGCAGGG CTACCGCGGC 1320CGTACTGGCA TCTACGAGCT GGTGATCTTC GACGACCAGA TGCGCACCCT GGTGCACAAC 1380GGCGCCGGTG AGCAGGAGCT GATTCGCCAC GCCCGCAGCC TCGGCCCGAG CATCCGCGAC 1440GATGGCCGGC GCAAGGTGCT GGAAGGGGTG ACCAGCCTGG AAGAAGTGTT GCGCGTGACC 1500CGGGAAGACT GA 1512 503 amino acids amino acid single linear unknown 7Met Ser Thr Asp Thr His Ala Ala Leu Thr Ala Pro Ala Ser Pro Ala 1 5 10 15Leu Arg Pro Leu Pro Phe Ala Phe Ala Lys Arg His Gly Val Leu Leu 20 25 30Arg Glu Pro Phe Gly Gln Val Gln Leu Gln Val Arg Arg Gly Ala Ser 35 40 45Leu Ala Ala Val Gln Glu Ala Gln Arg Phe Ala Gly Arg Val Leu Pro 50 55 60Leu His Trp Leu Glu Pro Glu Ala Phe Glu Gln Glu Leu Ala Leu Ala65 70 75 80Tyr Gln Arg Asp Ser Ser Glu Val Arg Gln Met Ala Glu Gly Met Gly 85 90 95Ala Glu Leu Asp Leu Ala Ser Leu Ala Glu Leu Thr Pro Glu Ser Gly 100 105 110Asp Leu Leu Glu Gln Glu Asp Asp Ala Pro Ile Ile Arg Leu Ile Asn 115 120 125Ala Ile Leu Ser Glu Ala Ile Lys Ala Gly Ala Ser Asp Ile His Leu 130 135 140Glu Thr Phe Glu Lys Arg Leu Val Val Arg Phe Arg Val Asp Gly Ile145 150 155 160Leu Arg Glu Val Ile Glu Pro Arg Arg Glu Leu Ala Ala Leu Leu Val 165 170 175Ser Arg Val Lys Val Met Ala Arg Leu Asp Ile Ala Glu Lys Arg Val 180 185 190Pro Gln Asp Gly Arg Ile Ser Leu Lys Val Gly Gly Arg Glu Val Asp 195 200 205Ile Arg Val Ser Thr Leu Pro Ser Ala Asn Gly Glu Arg Val Val Leu 210 215 220Arg Leu Leu Asp Lys Gln Ala Gly Arg Leu Ser Leu Thr His Leu Gly225 230 235 240Met Ser Glu Arg Asp Arg Arg Leu Leu Asp Asp Asn Leu Arg Lys Pro 245 250 255His Gly Ile Ile Leu Val Thr Gly Pro Thr Gly Ser Gly Lys Thr Thr 260 265 270Thr Leu Tyr Ala Gly Leu Val Thr Leu Asn Asp Arg Ser Arg Asn Ile 275 280 285Leu Thr Val Glu Asp Pro Ile Glu Tyr Tyr Leu Glu Gly Ile Gly Gln 290 295 300Thr Gln Val Asn Pro Arg Val Asp Met Thr Phe Ala Arg Gly Leu Arg305 310 315 320Ala Ile Leu Arg Gln Asp Pro Asp Val Val Met Val Gly Glu Ile Arg 325 330 335Asp Gln Glu Thr Ala Asp Ile Ala Val Gln Ala Ser Leu Thr Gly His 340 345 350Leu Val Leu Ser Thr Leu His Thr Asn Ser Ala Val Gly Ala Val Thr 355 360 365Arg Leu Val Asp Met Gly Val Glu Pro Phe Leu Leu Ser Ser Ser Leu 370 375 380Leu Gly Val Leu Ala Gln Arg Leu Val Arg Val Leu Cys Val His Cys385 390 395 400Arg Glu Ala Arg Pro Ala Asp Ala Ala Glu Cys Gly Leu Leu Gly Leu 405 410 415Asp Pro His Ser Gln Pro Leu Ile Tyr His Ala Lys Gly Cys Pro Glu 420 425 430Cys His Gln Gln Gly Tyr Arg Gly Arg Thr Gly Ile Tyr Glu Leu Val 435 440 445Ile Phe Asp Asp Gln Met Arg Thr Leu Val His Asn Gly Ala Gly Glu 450 455 460Gln Glu Leu Ile Arg His Ala Arg Ser Leu Gly Pro Ser Ile Arg Asp465 470 475 480Asp Gly Arg Arg Lys Val Leu Glu Gly Val Thr Ser Leu Glu Glu Val 485 490 495Leu Arg Val Thr Arg Glu Asp 500 1215 base pairs nucleic acid single linear unknown 8ATGGCCGCCT TCGAATACAT CGCCCTGGAT GCCAGGGGCC GCCAGCAGAA GGGCGTGCTG 60GAGGGCGACA GCGCCCGCCA GGTGCGCCAG CTGCTGCGCG ACAAACAGTT GTCGCCGCTG 120CAGGTCGAGC CGGTACAGCG CAGGGAGCAG GCCGAGGCTG GTGGCTTCAG CCTGCGCCGT 180GGCCTGTCGG CGCGCGACCT GGCGCTGGTC ACCCGTCAGC TGGCGACCCT GATCGGCGCC 240GCGCTGCCCA TCGAGGAAGC GCTGCGCGCC GCCGCCGCGC AGTCGCGCCA GCCGCGCATC 300CAGTCGATGC TGTTGGCGGT GCGCGCCAAG GTGCTCGAGG GCCACAGCCT GGCCAAGGCC 360CTGGCCTCCT ACCCGGCGGC CTTCCCCGAG CTGTACCGCG CCACGGTGGC GGCCGGCGAG 420CATGCGGGGC ACCTGGCGCC GGTGCTGGAG CAGCTGGCCG ACTACACCGA GCAGCGCCAG 480CAGTCGCGGC AGAAGATCCA GATGGCGCTG CTCTACCCGG TGATCCTGAT GCTCGCTTCG 540CTGGGCATCG TCGGTTTTCT GCTCGGCTAC GTGGTGCCGG ATGTGGTGCG GGTGTTCGTC 600GACTCCGGGC AGACCCTGCC GGCGCTGACC CGCGGGCTGA TTTTCCTCAG CGAGCTGGTC 660AAGTCCTGGG GCGCCCTGGC CATCGTCCTG GCGGTGCTCG GCGTGCTCGC CTTTCGCCGC 720GCCTTGCGCA GCGAGGATCT GCGCCGGCGC TGGCATGCCT TCCTGCTGCG CGTGCCGCTG 780GTCGGTGGGC TGATCGCCGC CACCGAGACG GCACGCTTCG CCTCGACCCT GGCCATCCTG 840GTGCGCAGCG GCGTGCCACT GGTGGAGGCG CTGGCCATCG GCGCCGAGGT GGTGTCCAAC 900CTGATCATCC GCAGCGACGT GGCCAACGCC ACCCAGCGCG TGCGCGAGGG CGGCAGCCTG 960TCGCGCGCGC TGGAAGCCAG CCGGCAGTTT CCGCCGATGA TGCTGCACAT GATCGCCAGC 1020GGCGAGCGTT CCGGCGAGCT GGACCAGATG CTGGCGCGCA CGGCGCGCAA CCAGGAAAAC 1080GACCTGGCGG CCACCATCGG CCTGCTGGTG GGGCTGTTCG AGCCGTTCAT GCTGGTATTC 1140ATGGGCGCGG TGGTGCTGGT GATCGTGCTG GCCATCCTGC TGCCGATTCT TTCTCTGAAC 1200CAACTGGTGG GTTGA 1215 404 amino acids amino acid single linear unknown 9Met Ala Ala Phe Glu Tyr Ile Ala Leu Asp Ala Arg Gly Arg Gln Gln 1 5 10 15Lys Gly Val Leu Glu Gly Asp Ser Ala Arg Gln Val Arg Gln Leu Leu 20 25 30Arg Asp Lys Gln Leu Ser Pro Leu Gln Val Glu Pro Val Gln Arg Arg 35 40 45Glu Gln Ala Glu Ala Gly Gly Phe Ser Leu Arg Arg Gly Leu Ser Ala 50 55 60Arg Asp Leu Ala Leu Val Thr Arg Gln Leu Ala Thr Leu Ile Gly Ala65 70 75 80Ala Leu Pro Ile Glu Glu Ala Leu Arg Ala Ala Ala Ala Gln Ser Arg 85 90 95Gln Pro Arg Ile Gln Ser Met Leu Leu Ala Val Arg Ala Lys Val Leu 100 105 110Glu Gly His Ser Leu Ala Lys Ala Leu Ala Ser Tyr Pro Ala Ala Phe 115 120 125Pro Glu Leu Tyr Arg Ala Thr Val Ala Ala Gly Glu His Ala Gly His 130 135 140Leu Ala Pro Val Leu Glu Gln Leu Ala Asp Tyr Thr Glu Gln Arg Gln145 150 155 160Gln Ser Arg Gln Lys Ile Gln Met Ala Leu Leu Tyr Pro Val Ile Leu 165 170 175Met Leu Ala Ser Leu Gly Ile Val Gly Phe Leu Leu Gly Tyr Val Val 180 185 190Pro Asp Val Val Arg Val Phe Val Asp Ser Gly Gln Thr Leu Pro Ala 195 200 205Leu Thr Arg Gly Leu Ile Phe Leu Ser Glu Leu Val Lys Ser Trp Gly 210 215 220Ala Leu Ala Ile Val Leu Ala Val Leu Gly Val Leu Ala Phe Arg Arg225 230 235 240Ala Leu Arg Ser Glu Asp Leu Arg Arg Arg Trp His Ala Phe Leu Leu 245 250 255Arg Val Pro Leu Val Gly Gly Leu Ile Ala Ala Thr Glu Thr Ala Arg 260 265 270Phe Ala Ser Thr Leu Ala Ile Leu Val Arg Ser Gly Val Pro Leu Val 275 280 285Glu Ala Leu Ala Ile Gly Ala Glu Val Val Ser Asn Leu Ile Ile Arg 290 295 300Ser Asp Val Ala Asn Ala Thr Gln Arg Val Arg Glu Gly Gly Ser Leu305 310 315 320Ser Arg Ala Leu Glu Ala Ser Arg Gln Phe Pro Pro Met Met Leu His 325 330 335Met Ile Ala Ser Gly Glu Arg Ser Gly Glu Leu Asp Gln Met Leu Ala 340 345 350Arg Thr Ala Arg Asn Gln Glu Asn Asp Leu Ala Ala Thr Ile Gly Leu 355 360 365Leu Val Gly Leu Phe Glu Pro Phe Met Leu Val Phe Met Gly Ala Val 370 375 380Val Leu Val Ile Val Leu Ala Ile Leu Leu Pro Ile Leu Ser Leu Asn385 390 395 400Gln Leu Val Gly 423 base pairs nucleic acid single linear unknown 10ATGTACAAAC AGAAAGGCTT CACGCTGATC GAAATCATGG TGGTGGTGGT CATCCTCGGC 60ATTCTCGCTG CCCTGGTGGT GCCGCAGGTG ATGGGCCGCC CGGACCAGGC CAAGGTCACC 120GCGGCGCAGA ACGACATCCG CGCCATCGGC GCCGCGCTGG ACATGTACAA GCTGGACAAC 180CAGAACTACC CGAGCACCCA GCAGGGCCTG GAGGCCCTGG TGAAGAAACC CACCGGCACG 240CCGGCGGCGA AGAACTGGAA CGCCGAGGGC TACCTGAAGA AGCTGCCGGT CGACCCCTGG 300GGCAACCAGT ACCTGTACCT GTCGCCGGGC ACCCGCGGCA AGATCGACCT GTATTCGCTG 360GGCGCCGACG GCCAGGAAGG CGGCGAGGGG ACCGACGCCG ACATCGGCAA CTGGGATCTC 420TGA 423 140 amino acids amino acid single linear unknown 11Met Tyr Lys Gln Lys Gly Phe Thr Leu Ile Glu Ile Met Val Val Val 1 5 10 15Val Ile Leu Gly Ile Leu Ala Ala Leu Val Val Pro Gln Val Met Gly 20 25 30Arg Pro Asp Gln Ala Lys Val Thr Ala Ala Gln Asn Asp Ile Arg Ala 35 40 45Ile Gly Ala Ala Leu Asp Met Tyr Lys Leu Asp Asn Gln Asn Tyr Pro 50 55 60Ser Thr Gln Gln Gly Leu Glu Ala Leu Val Lys Lys Pro Thr Gly Thr65 70 75 80Pro Ala Ala Lys Asn Trp Asn Ala Glu Gly Tyr Leu Lys Lys Leu Pro 85 90 95Val Asp Pro Trp Gly Asn Gln Tyr Leu Tyr Leu Ser Pro Gly Thr Arg 100 105 110Gly Lys Ile Asp Leu Tyr Ser Leu Gly Ala Asp Gly Gln Glu Gly Gly 115 120 125Glu Gly Thr Asp Ala Asp Ile Gly Asn Trp Asp Leu 130 135 140 642 base pairs nucleic acid single linear unknown 12TTGAGTAGCA CCCGCACCCG CCTGCCCGCC TGGCTGCAGC GCCACGGCGT GACCGGCCTC 60TGCCTGCTCG TGGTGCTGCT CATCACCCTC AGCCTGAGCA AGCAGAGCAT CGACTTCCTT 120CGCCTGCTGC GCAGCGAGGC CGCGCCACCG CCCGCCCCAG AGAGCATCGC CGAGCGCCAG 180CCGCTGTCCA TCCAGCGCCT GCAGCATCTG TTCGGCACGC CCGCGGCCAG GCCGCGCGGC 240GACCAGGCCG CCCCCGCCAC CCGGCAGCAG ATGACCCTGC TGGCCAGCTT CGTCAACCCG 300GACGCCAAGC GCTCCACGGC GATCATCCAG GTCGCCGGCG ACAAACCCAA GCGCATCGCC 360GTGGGCGAAT CGGTCAACGT CAGCACCCGC CTGCAGGCCG TCTATCAGGA CCACGTGGTG 420CTCGACCGCG GCGGCGTCGA GGAGAGCCTG CGCTTCCCCG CCGTGCGCCA GCCCTCTCTG 480ACGCCGGCCT ACTCGGCGCT GGAGCCCACC GCCAGCCAAC TGGAACAGCT GCAGGACGAA 540GACGTCCAGG CCCTGCAGGA GCGCATCCAG ACCCTTCAAC AACGCATGGA AGGCGGCGAC 600ATCCCGCAGC CCGAAATACC GGAAGCCGAA GACAGCCCAT GA 642 213 amino acids amino acid single linear unknown 13Met Ser Ser Thr Arg Thr Arg Leu Pro Ala Trp Leu Gln Arg His Gly 1 5 10 15Val Thr Gly Leu Cys Leu Leu Val Val Leu Leu Ile Thr Leu Ser Leu 20 25 30Ser Lys Gln Ser Ile Asp Phe Leu Arg Leu Leu Arg Ser Glu Ala Ala 35 40 45Pro Pro Pro Ala Pro Glu Ser Ile Ala Glu Arg Gln Pro Leu Ser Ile 50 55 60Gln Arg Leu Gln His Leu Phe Gly Thr Pro Ala Ala Arg Pro Arg Gly65 70 75 80Asp Gln Ala Ala Pro Ala Thr Arg Gln Gln Met Thr Leu Leu Ala Ser 85 90 95Phe Val Asn Pro Asp Ala Lys Arg Ser Thr Ala Ile Ile Gln Val Ala 100 105 110Gly Asp Lys Pro Lys Arg Ile Ala Val Gly Glu Ser Val Asn Val Ser 115 120 125Thr Arg Leu Gln Ala Val Tyr Gln Asp His Val Val Leu Asp Arg Gly 130 135 140Gly Val Glu Glu Ser Leu Arg Phe Pro Ala Val Arg Gln Pro Ser Leu145 150 155 160Thr Pro Ala Tyr Ser Ala Leu Glu Pro Thr Ala Ser Gln Leu Glu Gln 165 170 175Leu Gln Asp Glu Asp Val Gln Ala Leu Gln Glu Arg Ile Gln Thr Leu 180 185 190Gln Gln Arg Met Glu Gly Gly Asp Ile Pro Gln Pro Glu Ile Pro Glu 195 200 205Ala Glu Asp Ser Pro 210 1950 base pairs nucleic acid single linear unknown 14ATGATCGACT CCAGAATTCC GCCGCACAAA CGCCTGCCCC TCGCCCTGCT GCTGGCCGCG 60AGCTGCCTCG CCGCCCCGCT GCCGCTCGTC CATGCCGCCG AGCCGGTGGC GGTGAGCCAG 120GGCGCCGAGA CCTGGACCAT CAACATGAAG GACGCCGATA TCCGCGACTT CATCGACCAG 180GTGGCGCAGA TCTCTGGCGA GACCTTCGTC GTCGATCCGC GGGTCAAGGG CCAGGTCACG 240GTGATCTCCA AGACCCCGCT GGGCCTCGAG GAGGTCTACC AGCTGTTCCT TTCGGTGATG 300AGCACCCATG GCTTCAGCGT GCTGGCACAG GGCGACCAGG CGCGCATCGT GCCGGTCACC 360GAGGCGCGTA GCGGCGCCAA CAGCAGCCGC AGCGCGCCGG ACGATGTGCA GACCGAGCTG 420ATCCAGGTGC AGCACACCTC GGTCAACGAA CTGATCCCGC TGATCCGCCC GCTGGTGCCG 480CAGAACGGCC ACCTGGCGGC GGTCGCCGCC TCCAACGCGC TGATCATCAG CGACCGCCGG 540GCNAATATCG AACGCATCCG CGAACTGATC GCCGAGCTCG ATGCCCAGGG CGGCGGCGAC 600TACAACGTGA TCAACCTGCA GCATGCCTGG GTACTGGACG CCGCCGAGGC ACTGAACAAC 660GCGGTGATGC GCAACGAGAA AAACAGCGCC GGCACCCGGG TGATTGCCGA CGCCCGCACC 720AACCGCCTGA TCCTCCTCGG CCCGCCGGCC GCCCGCCAGC GCCTGGCCAA CCTGGCCCGC 780TCGCTGGACA TCCCCAGCAC CCGTTCGGCC AATGCGCGGG TAATTCGCCT ACGCCACAGC 840GACGCCAAGA GCCTGGCCGA GACCCTGGGC GACATCTCCG AGGGGTTGAA GACCGCGGAG 900GGTGGTGGCG AAGCCGCCAG CAGCAAGCCG CAGAACATCC TGATCCGCGC CGACGAGAGC 960CTCAATGCCC TGGTCCTGCT GGCCGATCCG GACACCGTGG CGACCCTCGA GGAAATCGTG 1020CGCAACCTCG ACGTGCCGCG CGCCCAGGTG ATGGTCGAGG CGGCCATCGT GGAAATCTCC 1080GGGGACATCA GCGACGCCCT CGGCGTGCAG TGGGCGGTGG ATGCCCGCGG CGGCACCGGC 1140GGCCTCGGCG GGGTCAACTT CGGCAATACC GGGCTATCGG TGGGCACCGT GCTCAAGGCC 1200ATCCAGAACG AGGAAATCCC CGATGACCTG ACCCTGCCGG ACGGCGCCAT CATCGGCATC 1260GGCACCGAGA ACTTCGGCGC GCTGATCACT GCCCTCTCTG CCAACAGCAA GAGCAACCTG 1320CTGTCCACGC CCAGCCTGCT GACCCTGGAC AACCAGGAGG CGGAAATCCT GGTCGGGCAG 1380AACGTGCCTT TCCAGACCGG CTCCTACACC ACCGACGCCT CGGGGGCGAA CAACCCCTTC 1440ACCACCATTG AGCGCGAGGA CATCGGCGTG ACCCTCAAGG TCACCCCGCA CATCAACGAC 1500GGCGCCACCC TGCGCCTGGA AGTGGAGCAG GAGATCTCCT CCATCGCCCC CAGCGCCGGG 1560GTCAATGCCC AGGCGGTGGA CCTGGTGACC AACAAGCGCT CGATCAAGAG CGTGATCCTG 1620GCCGACGACG GCCAGGTCAT AGTGCTGGGA GGGCTGATCC AGGACGACGT CACCAGCACC 1680GACTCCAAGG TGCCGCTGCT GGGTGACATC CCGCTGATCG GCCGGCTGTT CCGCTCGACC 1740AAGGACACCC ACGTCAAGCG CAACCTGATG GTGTTCCTGC GCCCGACCAT CGTCCGCGAC 1800CGCGCCGGCA TGGCCGCGCT GTCGGGCAAG AAGTACAGCG ACATCAGCGT GCTGGGTGCC 1860GACGAGGATG GCCACAGCAG CCTGCCGGGC AGCGCCGAGC GCCTGTTCGA CAAACCCGGC 1920GCCGGTGCCG TGGACCTGCG CGACCAGTGA 1950 649 amino acids amino acid single linear unknown 15Met Ile Asp Ser Arg Ile Pro Pro His Lys Arg Leu Pro Leu Ala Leu 1 5 10 15Leu Leu Ala Ala Ser Cys Leu Ala Ala Pro Leu Pro Leu Val His Ala 20 25 30Ala Glu Pro Val Ala Val Ser Gln Gly Ala Glu Thr Trp Thr Ile Asn 35 40 45Met Lys Asp Ala Asp Ile Arg Asp Phe Ile Asp Gln Val Ala Gln Ile 50 55 60Ser Gly Glu Thr Phe Val Val Asp Pro Arg Val Lys Gly Gln Val Thr65 70 75 80Val Ile Ser Lys Thr Pro Leu Gly Leu Glu Glu Val Tyr Gln Leu Phe 85 90 95Leu Ser Val Met Ser Thr His Gly Phe Ser Val Leu Ala Gln Gly Asp 100 105 110Gln Ala Arg Ile Val Pro Val Thr Glu Ala Arg Ser Gly Ala Asn Ser 115 120 125Ser Arg Ser Ala Pro Asp Asp Val Gln Thr Glu Leu Ile Gln Val Gln 130 135 140His Thr Ser Val Asn Glu Leu Ile Pro Leu Ile Arg Pro Leu Val Pro145 150 155 160Gln Asn Gly His Leu Ala Ala Val Ala Ala Ser Asn Ala Leu Ile Ile 165 170 175Ser Asp Arg Arg Ala Asn Ile Glu Arg Ile Arg Glu Leu Ile Ala Glu 180 185 190Leu Asp Ala Gln Gly Gly Gly Asp Tyr Asn Val Ile Asn Leu Gln His 195 200 205Ala Trp Val Leu Asp Ala Ala Glu Ala Leu Asn Asn Ala Val Met Arg 210 215 220Asn Glu Lys Asn Ser Ala Gly Thr Arg Val Ile Ala Asp Ala Arg Thr225 230 235 240Asn Arg Leu Ile Leu Leu Gly Pro Pro Ala Ala Arg Gln Arg Leu Ala 245 250 255Asn Leu Ala Arg Ser Leu Asp Ile Pro Ser Thr Arg Ser Ala Asn Ala 260 265 270Arg Val Ile Arg Leu Arg His Ser Asp Ala Lys Ser Leu Ala Glu Thr 275 280 285Leu Gly Asp Ile Ser Glu Gly Leu Lys Thr Ala Glu Gly Gly Gly Glu 290 295 300Ala Ala Ser Ser Lys Pro Gln Asn Ile Leu Ile Arg Ala Asp Glu Ser305 310 315 320Leu Asn Ala Leu Val Leu Leu Ala Asp Pro Asp Thr Val Ala Thr Leu 325 330 335Glu Glu Ile Val Arg Asn Leu Asp Val Pro Arg Ala Gln Val Met Val 340 345 350Glu Ala Ala Ile Val Glu Ile Ser Gly Asp Ile Ser Asp Ala Leu Gly 355 360 365Val Gln Trp Ala Val Asp Ala Arg Gly Gly Thr Gly Gly Leu Gly Gly 370 375 380Val Asn Phe Gly Asn Thr Gly Leu Ser Val Gly Thr Val Leu Lys Ala385 390 395 400Ile Gln Asn Glu Glu Ile Pro Asp Asp Leu Thr Leu Pro Asp Gly Ala 405 410 415Ile Ile Gly Ile Gly Thr Glu Asn Phe Gly Ala Leu Ile Thr Ala Leu 420 425 430Ser Ala Asn Ser Lys Ser Asn Leu Leu Ser Thr Pro Ser Leu Leu Thr 435 440 445Leu Asp Asn Gln Glu Ala Glu Ile Leu Val Gly Gln Asn Val Pro Phe 450 455 460Gln Thr Gly Ser Tyr Thr Thr Asp Ala Ser Gly Ala Asn Asn Pro Phe465 470 475 480Thr Thr Ile Glu Arg Glu Asp Ile Gly Val Thr Leu Lys Val Thr Pro 485 490 495His Ile Asn Asp Gly Ala Thr Leu Arg Leu Glu Val Glu Gln Glu Ile 500 505 510Ser Ser Ile Ala Pro Ser Ala Gly Val Asn Ala Gln Ala Val Asp Leu 515 520 525Val Thr Asn Lys Arg Ser Ile Lys Ser Val Ile Leu Ala Asp Asp Gly 530 535 540Gln Val Ile Val Leu Gly Gly Leu Ile Gln Asp Asp Val Thr Ser Thr545 550 555 560Asp Ser Lys Val Pro Leu Leu Gly Asp Ile Pro Leu Ile Gly Arg Leu 565 570 575Phe Arg Ser Thr Lys Asp Thr His Val Lys Arg Asn Leu Met Val Phe 580 585 590Leu Arg Pro Thr Ile Val Arg Asp Arg Ala Gly Met Ala Ala Leu Ser 595 600 605Gly Lys Lys Tyr Ser Asp Ile Ser Val Leu Gly Ala Asp Glu Asp Gly 610 615 620His Ser Ser Leu Pro Gly Ser Ala Glu Arg Leu Phe Asp Lys Pro Gly625 630 635 640Ala Gly Ala Val Asp Leu Arg Asp Gln 645 2742 base pairs nucleic acid single linear unknown 16ATGTCTGTTT GGGTCACGTG GCCGGGCTTG GTCAAGTTCG GCACCCTGGG CATCTATGCC 60GGCCTGATCA CGCTCGCGCT TGAGCGCGAC GTGCTGTTCA AGAACAACCT GTTCGACGTC 120GACAACCTGC CCGCGGCCAA CGCCAGCATC ACCTGTGATG CCCGCAGCCA GGTGGCGCGT 180ACCGAGGACG GCACCTGTAA CATCCTCGCC AACCCGGCCG AGGGCTCGGT GTACCGCCGC 240TTCGGGCGCA ACGTCGACCC CAGCGTGACC CATGGCGAGA CCGAGGCCGA CACCCTGCTC 300AGTCCCAATC CGCGGGAGGT GAGTAACGTG CTGATGGCGC GTGGCGAGTT CAAGCCGGCG 360CCCAGCCTCA ACTTCATCGC CGCCTCCTGG ATCCAGTTCA TGGTGCATGA CTGGGTCGAA 420CACGGCCCCA ACGCCGAAGC CAACCCGATC CAGGTGCCGC TGCCGGCTGG CGACGCGCTC 480GGCTCCGGCA GCCTGTCCGT GCGCCGCACC CAGCCCGACC CGACCCGTAC CCCGGCCGAG 540GCCGGCAAGC CGGCCACCTA CCGCAACCAC AACACCCACT GGTGGGATGG CTCGCAGTTG 600TATGGCAGCA GCAAGGACAT CAACGACAAG GTGCGCGCCT TCGAGGGTGG CAAGCTGAAG 660ATCAATCCCG ACGGTACCCT GCCGACCGAG TTCCTCAGCG GCAAGCCGAT CACCGGCTTC 720AACGAGAACT GGTGGGTTGG CCTGAGCATG CTGCACCAGC TGTTCACTAA GGAGCACAAC 780GCCATCGCGG CGATGCTCCA GCAGAAGTAC CCGGACAAGG ACGACCAGTG GCTGTACGAC 840CATGCGCGCC TGGTCAACTC CGCGCTGATG GCCAAGATCC ACACCGTGGA ATGGACCCCG 900GCGGTGATCG CCAACCCGGT CACCGAACGC GCCATGTATG CCAACTGGTG GGGCCTGCTG 960GGTTCCGGTC CGGAGCGTGA CAAGTACCAG GAAGAGGCGC GCATGCTGCA GGAGGACCTG 1020GCCAGCTCCA ACTCCTTCGT CCTGCGCATT CTCGGCATCG ACGGCAGCCA GGCCGGCAGT 1080TCGGCCATCG ACCATGCCCT GGCCGGCATC GTCGGCTCGA CCAACCCGAA CAACTACGGC 1140GTGCCCTACA CCCTGACCGA GGAGTTCGTC GCGGTCTACC GCATGCACCC GCTGATGCGC 1200GACAAGGTCG ATGTCTACGA CATCGGCTCG AACATCATCG CGCGCAGCGT GCCGCTGCAG 1260GAGACCCGCG ATGCCGACGC CGAGGAGCTG CTGGCGGACG AGAATCCCGA GCGCCTGTGG 1320TACTCCTTCG GCATCACCAA CCCGGGCTCG CTGACCCTCA ACAACTACCC GAACTTCCTG 1380CGCAACCTGT CCATGCCGCT GGTCGGCAAC ATCGACCTGG CGACCATCGA CGTGCTGTGT 1440GACCGCGAGC GCGGGGTGCC GCGCTACAAC GAGTTCCGCC GCGAGATCGG CCTCAACCCG 1500ATCACCAAGT TGGAGGACCT GACCACCGAC CCGGCCACCC TGGCCAACCT CAAGCGCATC 1560TACGGCAACG ACATCGAGAA GATTGACACC CTGGTCGGCA TGCTGGCCGA GACCGTGCGT 1620CCGGACGGCT TCGCCTTCGG CGAGACGGCC TTCCAGATCT TCATCATGAA CGCCTCGCGG 1680CGCCTGATGA CCGACCGCTT CTATACCAAG GACTACCGCC CGGAGATCTA CACCGCCGAG 1740GGCCTGGCCT GGGTCGAGAA CACCACCATG GTCGACGTGC TCAAACGCCA CAATCCGCAG 1800CTGGTCAACA GCCTGGTTGG CGTGGAAAAC GCCTTCAAAC CCTGGGGCCT GAACATCCCG 1860GCCGACTACG AGAGCTGGCC GGGCAAGGCC AAGCAGGACA ACCTGTGGGT CAACGGCGCC 1920NTGCGCACCC AGTACGCCGC AGGCCAGCTG CCGGCCATTC CGCCGGTGGA CGTCGGCGGC 1980CTGATCAGTT CGGTGCTGTG GAAGAAGGTG CAGACCAANT CCGACGTGGC GCCGGCCGGC 2040TACGAGAAGG CCATGCACCC GCATGGCGTG ATGGCCAAGG TCAAGTTCAC CGCCGTGCCG 2100GGGCACCCCT ACACCGGCCT GTTCCAGGGT GCCGACAGCG GCCTGCTGCG CCTGTCGGTG 2160GCCGGCGACC CGGCAACCAA CGGCTTCCAG CCGGGTCTGG CGTGGAAGGC CTTCGTCGAC 2220GGCAAGCCGT CGCAGAACGT CTCCGCGCTC TACACCCTGA GCGGGCAGGG CAGCAACCAC 2280AACTTCTTCG CCAACGAGCT GTCGCAGTTC GTCCTGCCGG AGACCAACGA TACCCTGGGC 2340ACCACGCTGC TGTTCTCGCT GGTCAGCCTC AAGCCGACCT TGCTGCGCGT GGACGACATG 2400GCCGAAGTGA CCCAGACCGG CCAGGCCGTG ACTTCGGTCA AGGCGCCGAC GCAGATCTAC 2460TTCGTGCCCA AGCCGGAGCT GCGCAGCCTG TTCTCCAGTG CGGCGCATGA CTTCCGCAGC 2520GACCTGACGA GCCTCACCGC CGGCACCAAG CTGTACGACG TCTACGCTAC CTCGATGGAG 2580ATCAAGACCT CGATCCTGCC GTCGACCAAT CGTAGCTACG CCCAGCAACG GCGCAACAGC 2640GCGGTGAAGA TCGGCGAGAT GGAGCTGACC TCGCCGTTCA TCGCCTCGGC CTTCGGCGAC 2700AACGGGGTGT TCTTCAAGCA CCAGCGTCAC GAAGACAAAT AA 2742 913 amino acids amino acid single linear unknown 17Met Ser Val Trp Val Thr Trp Pro Gly Leu Val Lys Phe Gly Thr Leu 1 5 10 15Gly Ile Tyr Ala Gly Leu Ile Thr Leu Ala Leu Glu Arg Asp Val Leu 20 25 30Phe Lys Asn Asn Leu Phe Asp Val Asp Asn Leu Pro Ala Ala Asn Ala 35 40 45Ser Ile Thr Cys Asp Ala Arg Ser Gln Val Ala Arg Thr Glu Asp Gly 50 55 60Thr Cys Asn Ile Leu Ala Asn Pro Ala Glu Gly Ser Val Tyr Arg Arg65 70 75 80Phe Gly Arg Asn Val Asp Pro Ser Val Thr His Gly Glu Thr Glu Ala 85 90 95Asp Thr Leu Leu Ser Pro Asn Pro Arg Glu Val Ser Asn Val Leu Met 100 105 110Ala Arg Gly Glu Phe Lys Pro Ala Pro Ser Leu Asn Phe Ile Ala Ala 115 120 125Ser Trp Ile Gln Phe Met Val His Asp Trp Val Glu His Gly Pro Asn 130 135 140Ala Glu Ala Asn Pro Ile Gln Val Pro Leu Pro Ala Gly Asp Ala Leu145 150 155 160Gly Ser Gly Ser Leu Ser Val Arg Arg Thr Gln Pro Asp Pro Thr Arg 165 170 175Thr Pro Ala Glu Ala Gly Lys Pro Ala Thr Tyr Arg Asn His Asn Thr 180 185 190His Trp Trp Asp Gly Ser Gln Leu Tyr Gly Ser Ser Lys Asp Ile Asn 195 200 205Asp Lys Val Arg Ala Phe Glu Gly Gly Lys Leu Lys Ile Asn Pro Asp 210 215 220Gly Thr Leu Pro Thr Glu Phe Leu Ser Gly Lys Pro Ile Thr Gly Phe225 230 235 240Asn Glu Asn Trp Trp Val Gly Leu Ser Met Leu His Gln Leu Phe Thr 245 250 255Lys Glu His Asn Ala Ile Ala Ala Met Leu Gln Gln Lys Tyr Pro Asp 260 265 270Lys Asp Asp Gln Trp Leu Tyr Asp His Ala Arg Leu Val Asn Ser Ala 275 280 285Leu Met Ala Lys Ile His Thr Val Glu Trp Thr Pro Ala Val Ile Ala 290 295 300Asn Pro Val Thr Glu Arg Ala Met Tyr Ala Asn Trp Trp Gly Leu Leu305 310 315 320Gly Ser Gly Pro Glu Arg Asp Lys Tyr Gln Glu Glu Ala Arg Met Leu 325 330 335Gln Glu Asp Leu Ala Ser Ser Asn Ser Phe Val Leu Arg Ile Leu Gly 340 345 350Ile Asp Gly Ser Gln Ala Gly Ser Ser Ala Ile Asp His Ala Leu Ala 355 360 365Gly Ile Val Gly Ser Thr Asn Pro Asn Asn Tyr Gly Val Pro Tyr Thr 370 375 380Leu Thr Glu Glu Phe Val Ala Val Tyr Arg Met His Pro Leu Met Arg385 390 395 400Asp Lys Val Asp Val Tyr Asp Ile Gly Ser Asn Ile Ile Ala Arg Ser 405 410 415Val Pro Leu Gln Glu Thr Arg Asp Ala Asp Ala Glu Glu Leu Leu Ala 420 425 430Asp Glu Asn Pro Glu Arg Leu Trp Tyr Ser Phe Gly Ile Thr Asn Pro 435 440 445Gly Ser Leu Thr Leu Asn Asn Tyr Pro Asn Phe Leu Arg Asn Leu Ser 450 455 460Met Pro Leu Val Gly Asn Ile Asp Leu Ala Thr Ile Asp Val Leu Cys465 470 475 480Asp Arg Glu Arg Gly Val Pro Arg Tyr Asn Glu Phe Arg Arg Glu Ile 485 490 495Gly Leu Asn Pro Ile Thr Lys Leu Glu Asp Leu Thr Thr Asp Pro Ala 500 505 510Thr Leu Ala Asn Leu Lys Arg Ile Tyr Gly Asn Asp Ile Glu Lys Ile 515 520 525Asp Thr Leu Val Gly Met Leu Ala Glu Thr Val Arg Pro Asp Gly Phe 530 535 540Ala Phe Gly Glu Thr Ala Phe Gln Ile Phe Ile Met Asn Ala Ser Arg545 550 555 560Arg Leu Met Thr Asp Arg Phe Tyr Thr Lys Asp Tyr Arg Pro Glu Ile 565 570 575Tyr Thr Ala Glu Gly Leu Ala Trp Val Glu Asn Thr Thr Met Val Asp 580 585 590Val Leu Lys Arg His Asn Pro Gln Leu Val Asn Ser Leu Val Gly Val 595 600 605Glu Asn Ala Phe Lys Pro Trp Gly Leu Asn Ile Pro Ala Asp Tyr Glu 610 615 620Ser Trp Pro Gly Lys Ala Lys Gln Asp Asn Leu Trp Val Asn Gly Ala625 630 635 640Xaa Arg Thr Gln Tyr Ala Ala Gly Gln Leu Pro Ala Ile Pro Pro Val 645 650 655Asp Val Gly Gly Leu Ile Ser Ser Val Leu Trp Lys Lys Val Gln Thr 660 665 670Xaa Ser Asp Val Ala Pro Ala Gly Tyr Glu Lys Ala Met His Pro His 675 680 685Gly Val Met Ala Lys Val Lys Phe Thr Ala Val Pro Gly His Pro Tyr 690 695 700Thr Gly Leu Phe Gln Gly Ala Asp Ser Gly Leu Leu Arg Leu Ser Val705 710 715 720Ala Gly Asp Pro Ala Thr Asn Gly Phe Gln Pro Gly Leu Ala Trp Lys 725 730 735Ala Phe Val Asp Gly Lys Pro Ser Gln Asn Val Ser Ala Leu Tyr Thr 740 745 750Leu Ser Gly Gln Gly Ser Asn His Asn Phe Phe Ala Asn Glu Leu Ser 755 760 765Gln Phe Val Leu Pro Glu Thr Asn Asp Thr Leu Gly Thr Thr Leu Leu 770 775 780Phe Ser Leu Val Ser Leu Lys Pro Thr Leu Leu Arg Val Asp Asp Met785 790 795 800Ala Glu Val Thr Gln Thr Gly Gln Ala Val Thr Ser Val Lys Ala Pro 805 810 815Thr Gln Ile Tyr Phe Val Pro Lys Pro Glu Leu Arg Ser Leu Phe Ser 820 825 830Ser Ala Ala His Asp Phe Arg Ser Asp Leu Thr Ser Leu Thr Ala Gly 835 840 845Thr Lys Leu Tyr Asp Val Tyr Ala Thr Ser Met Glu Ile Lys Thr Ser 850 855 860Ile Leu Pro Ser Thr Asn Arg Ser Tyr Ala Gln Gln Arg Arg Asn Ser865 870 875 880Ala Val Lys Ile Gly Glu Met Glu Leu Thr Ser Pro Phe Ile Ala Ser 885 890 895Ala Phe Gly Asp Asn Gly Val Phe Phe Lys His Gln Arg His Glu Asp 900 905 910Lys 525 base pairs nucleic acid single linear unknown 18ATGCAGCGGG GGCGCGGTTT CACTCTGATC GAGCTGCTGG TGGTGCTGGT GCTGCTGGGC 60GTGCTCACCG GCCTCGCCGT GCTCGGCAGC GGGATCGCCA GCAGCCCCGC GCGCAAGCTG 120GCGGACGAGG CCGAGCGCCT GCAGTCGCTG CTGCGGGTGC TGCTCGACGA GGCGGTGCTG 180GACAACCGCG AGTATGGCGT ACGCTTCGAC GCCCGGAGCT ACCGGGTGCT GCGCTTCGAG 240CCGCGCACGG CGCGCTGGGA GCCGCTCGAC GAGCGCGTGC ACGAGCTGCC GGAGTGGCTC 300GAGCTGGAGA TCGAGGTCGA CGAGCAGAGT GTCGGGCTGC CCGCCGCCCG TGGCGAGCAG 360GACAAAGCCG CGGCCAAGGC GCCACAGCTG CTGCTGCTCT CCAGTGGCGA GCTGACCCCC 420TTCGCCCTGC GCCTGTCCGC CGGCCGCGAG CGCGGCGCGC CGGTGCTGAC GCTGGCCAGC 480GACGGCTTCG CCGAGCCCGA GCTGCAGCAG GAAAAGTCCC GATGA 525 174 amino acids amino acid single linear unknown 19Met Gln Arg Gly Arg Gly Phe Thr Leu Ile Glu Leu Leu Val Val Leu 1 5 10 15Val Leu Leu Gly Val Leu Thr Gly Leu Ala Val Leu Gly Ser Gly Ile 20 25 30Ala Ser Ser Pro Ala Arg Lys Leu Ala Asp Glu Ala Glu Arg Leu Gln 35 40 45Ser Leu Leu Arg Val Leu Leu Asp Glu Ala Val Leu Asp Asn Arg Glu 50 55 60Tyr Gly Val Arg Phe Asp Ala Arg Ser Tyr Arg Val Leu Arg Phe Glu65 70 75 80Pro Arg Thr Ala Arg Trp Glu Pro Leu Asp Glu Arg Val His Glu Leu 85 90 95Pro Glu Trp Leu Glu Leu Glu Ile Glu Val Asp Glu Gln Ser Val Gly 100 105 110Leu Pro Ala Ala Arg Gly Glu Gln Asp Lys Ala Ala Ala Lys Ala Pro 115 120 125Gln Leu Leu Leu Leu Ser Ser Gly Glu Leu Thr Pro Phe Ala Leu Arg 130 135 140Leu Ser Ala Gly Arg Glu Arg Gly Ala Pro Val Leu Thr Leu Ala Ser145 150 155 160Asp Gly Phe Ala Glu Pro Glu Leu Gln Gln Glu Lys Ser Arg 165 170 390 base pairs nucleic acid single linear unknown 20ATGAAGCGCG GCCGCGGCTT CACCCTGCTC GAGGTGCTGG TGGCCCTGGC GATCTTCGCC 60GTGGTCGCCG CCAGCGTGCT CAGCGCCAGC GCTCGCTCGC TGAAGACCGC CGCGCGCCTG 120GAGGACAAGA CCTTCGCCAC CTGGCTGGCG GACAACCGCC TGCAGGAGCT GCAGCTGGCC 180GACGTGCCGC CGGGCGAGGG CCGCGAGCAG GGCGAGGAGA GCTACGCCGG GCGGCGCTGG 240CTGTGGCAGA GCGAGGTGCA GGCCACCAGC GAGCCGGAGA TGCTGCGTGT CACCGTACGG 300GTGGCGCTGC GGCCGGAGCG CGGGCTGCAG GGCAAGATCG AAGACCATGC CCTGGTGACC 360CTGAGTGGCT TCGTCGGGGT CGAGCCATGA 390 129 amino acids amino acid single linear unknown 21Met Lys Arg Gly Arg Gly Phe Thr Leu Leu Glu Val Leu Val Ala Leu 1 5 10 15Ala Ile Phe Ala Val Val Ala Ala Ser Val Leu Ser Ala Ser Ala Arg 20 25 30Ser Leu Lys Thr Ala Ala Arg Leu Glu Asp Lys Thr Phe Ala Thr Trp 35 40 45Leu Ala Asp Asn Arg Leu Gln Glu Leu Gln Leu Ala Asp Val Pro Pro 50 55 60Gly Glu Gly Arg Glu Gln Gly Glu Glu Ser Tyr Ala Gly Arg Arg Trp65 70 75 80Leu Trp Gln Ser Glu Val Gln Ala Thr Ser Glu Pro Glu Met Leu Arg 85 90 95Val Thr Val Arg Val Ala Leu Arg Pro Glu Arg Gly Leu Gln Gly Lys 100 105 110Ile Glu Asp His Ala Leu Val Thr Leu Ser Gly Phe Val Gly Val Glu 115 120 125Pro 684 base pairs nucleic acid single linear unknown 22ATGAGGCAGC GCGGCTTCAC CCTGCTGGAA GTGCTGATCG CCATCGCCAT CTTCGCCCTG 60CTGGCCATGG CCACCTACCG CATGCTCGAC AGCGTGCTGC AGACCGATCG TGGCCAGCGC 120CAGCAGGAGC AGCGTCTGCG CGAGCTGACG CGGGCCATGG CAGCTTTCGA ACGCGACCTG 180CTGCAGGTGC GCCTGCGTCC GGTGCGCGAC CCGCTGGGCG ACCTGCTGCC AGCCCTGCGC 240GGCAGCAGTG GCCGCGACAC CCAGCTGGAG TTCACCCGCA GCGGCTGGCG CAACCCGCTC 300GGCCAGCCGC GCGCCACCCT ACAGCGGGTG CGCTGGCAGC TCGAAGGCGA GCGCTGGCAG 360CGCGCTTACT GGACGGTGCT GGACCAGGCC CAGGACAGCC AGCCGCGGGT GCAGCAGGCG 420CTGGATGGCG TGCGCCGCTT CGACTTGCGC TTTCTCGACC AGGAGGGGCG CTGGCTGCAG 480GACTGGCCGC CGGCCAACAG TGCTGCCGAC GAGGCCCTGA CCCAGCTGCC GCGTGCCGTC 540GAGCTGGTCG TCGAGCACCG CCATTACGGT GAACTGCGCC GTCTCTGGCG CTTGCCCGAG 600ATGCCGCAGC AGGAACAGAT CACGCCGCCC GGGGGCGAGC AGGGCGGTGA GCTGCTGCCG 660GAAGAGCCGG AGCCCGAGGC ATGA 684 227 amino acids amino acid single linear unknown 23Met Arg Gln Arg Gly Phe Thr Leu Leu Glu Val Leu Ile Ala Ile Ala 1 5 10 15Ile Phe Ala Leu Leu Ala Met Ala Thr Tyr Arg Met Leu Asp Ser Val 20 25 30Leu Gln Thr Asp Arg Gly Gln Arg Gln Gln Glu Gln Arg Leu Arg Glu 35 40 45Leu Thr Arg Ala Met Ala Ala Phe Glu Arg Asp Leu Leu Gln Val Arg 50 55 60Leu Arg Pro Val Arg Asp Pro Leu Gly Asp Leu Leu Pro Ala Leu Arg65 70 75 80Gly Ser Ser Gly Arg Asp Thr Gln Leu Glu Phe Thr Arg Ser Gly Trp 85 90 95Arg Asn Pro Leu Gly Gln Pro Arg Ala Thr Leu Gln Arg Val Arg Trp 100 105 110Gln Leu Glu Gly Glu Arg Trp Gln Arg Ala Tyr Trp Thr Val Leu Asp 115 120 125Gln Ala Gln Asp Ser Gln Pro Arg Val Gln Gln Ala Leu Asp Gly Val 130 135 140Arg Arg Phe Asp Leu Arg Phe Leu Asp Gln Glu Gly Arg Trp Leu Gln145 150 155 160Asp Trp Pro Pro Ala Asn Ser Ala Ala Asp Glu Ala Leu Thr Gln Leu 165 170 175Pro Arg Ala Val Glu Leu Val Val Glu His Arg His Tyr Gly Glu Leu 180 185 190Arg Arg Leu Trp Arg Leu Pro Glu Met Pro Gln Gln Glu Gln Ile Thr 195 200 205Pro Pro Gly Gly Glu Gln Gly Gly Glu Leu Leu Pro Glu Glu Pro Glu 210 215 220Pro Glu Ala225 954 base pairs nucleic acid single linear unknown 24ATGAGCCGGC AGCGCGGCGT GGCACTGATC ACCGTGCTGC TGGTGGTGGC GCTGGTGACC 60GTGGTCTGCG CGGCCCTGCT GCTGCGCCAG CAGCTGGCCA TCCGCAGCAC CGGCAACCAG 120CTGCTGGTGC GCCAGGCCCA GTACTACGCC GAAGGCGGCG AGCTGCTGGC CAAGGCCCTG 180CTGCGTCGCG ACCTGGCCGC CGACCAGGTC GATCATCCCG GCGAGCCCTG GGCCAACCCC 240GGCCTGCGCT TCCCCCTGGA TGAGGGCGGC GAGCTGCGCC TGCGCATCGA GGACCTGGCC 300GGACGTTTCA ACCTCAACAG CCTGGCCGCC GGTGGTGAGG CCGGTGAGTT GGCGCTGCTG 360CGCCTGCGGC GCCTGCTGCA GCTGCTGCAG CTGACCCCGG CCTATGCCGA GCGCCTGCAG 420GACTGGCTCG ACGGCGATCA GGAGGCCAGC GGCATGGCCG GCGCCGAGGA TGACCAGTAC 480CTGCTGCAGA AACCGCCCTA CCGTACCGGC CCCGGGCGCA TTGCCGAGGT GTCGGAGCTG 540CGCCTGCTGC TGGGCATGAG CGAGGCCGAC TACCGCCGCC TGGCCCCCTT CGTCAGCGCC 600CTGCCGAGCC AGGTCGAGCT GAACATCAAC ACCGCCAGCG CCCTGGTGCT GGCTTGCCTG 660GGCGAGGGCA TNCCCGAGGC GGTGCTCGAG GCCGCCATCG ANGGTCGCGG CCGCAGCGGC 720TATCGCGAGC CCGCTGCCTT CGTCCAGCAN CTTGCCAGCT ACGGCGTCAG CCCGCAGGGG 780CTGGGCATCG CCAGCCAGTA TTTCCGTGTC ACCACCGAGG TGCTGCTGGG TGAGCGGCGC 840CAGGTGCTGG CCAGTTATCT GCAACGTGGT AATGATGGGC GCGTCCGCCT GATGGCGCGC 900GATCTGGGGC AGGAGGGCCT GGCGCCCCCA CCCGTCGAGG AGTCCGAGAA ATGA 954 317 amino acids amino acid single linear unknown 25Met Ser Arg Gln Arg Gly Val Ala Leu Ile Thr Val Leu Leu Val Val 1 5 10 15Ala Leu Val Thr Val Val Cys Ala Ala Leu Leu Leu Arg Gln Gln Leu 20 25 30Ala Ile Arg Ser Thr Gly Asn Gln Leu Leu Val Arg Gln Ala Gln Tyr 35 40 45Tyr Ala Glu Gly Gly Glu Leu Leu Ala Lys Ala Leu Leu Arg Arg Asp 50 55 60Leu Ala Ala Asp Gln Val Asp His Pro Gly Glu Pro Trp Ala Asn Pro65 70 75 80Gly Leu Arg Phe Pro Leu Asp Glu Gly Gly Glu Leu Arg Leu Arg Ile 85 90 95Glu Asp Leu Ala Gly Arg Phe Asn Leu Asn Ser Leu Ala Ala Gly Gly 100 105 110Glu Ala Gly Glu Leu Ala Leu Leu Arg Leu Arg Arg Leu Leu Gln Leu 115 120 125Leu Gln Leu Thr Pro Ala Tyr Ala Glu Arg Leu Gln Asp Trp Leu Asp 130 135 140Gly Asp Gln Glu Ala Ser Gly Met Ala Gly Ala Glu Asp Asp Gln Tyr145 150 155 160Leu Leu Gln Lys Pro Pro Tyr Arg Thr Gly Pro Gly Arg Ile Ala Glu 165 170 175Val Ser Glu Leu Arg Leu Leu Leu Gly Met Ser Glu Ala Asp Tyr Arg 180 185 190Arg Leu Ala Pro Phe Val Ser Ala Leu Pro Ser Gln Val Glu Leu Asn 195 200 205Ile Asn Thr Ala Ser Ala Leu Val Leu Ala Cys Leu Gly Glu Gly Xaa 210 215 220Pro Glu Ala Val Leu Glu Ala Ala Ile Xaa Gly Arg Gly Arg Ser Gly225 230 235 240Tyr Arg Glu Pro Ala Ala Phe Val Gln Xaa Leu Ala Ser Tyr Gly Val 245 250 255Ser Pro Gln Gly Leu Gly Ile Ala Ser Gln Tyr Phe Arg Val Thr Thr 260 265 270Glu Val Leu Leu Gly Glu Arg Arg Gln Val Leu Ala Ser Tyr Leu Gln 275 280 285Arg Gly Asn Asp Gly Arg Val Arg Leu Met Ala Arg Asp Leu Gly Gln 290 295 300Glu Gly Leu Ala Pro Pro Pro Val Glu Glu Ser Glu Lys305 310 315 1146 base pairs nucleic acid single linear unknown 26ATGAGTCTGC TCACCCTGTT TCTGCCGCCC CAGGCCTGCA CCGAGGCGAG CGCCGACATG 60CCGGTGTGGT GCGTCGAGAG CGACAGCTGC CGTCAGCTGC CCTTCGCCGA GGCCTTGCCG 120GCCGACGCGC GGGTCTGGCG CTTGGTGCTG CCGGTGGAGG CGGTGACCAC CTGTGTCGTG 180CAGTTGCCGA CCACCAAGGC ACGCTGGCTG GCCAAGGCCC TGCCGTTCGC CGTCGAGGAG 240CTGCTGGCCG AGGAGGTGGA GCAGTTTCAC CTGTGCGTCG GTAGCGCGCT GGTCGATGGT 300CGTCATCGTG TTCATGCCCT GCGCCGCGAG TGGCTGGCCG GCTGGCTGGC GCTGTGCGGC 360GAGCGGCCGC CGCAGTGGAT CGAGGTGGAC GCCGACCTGT TGCCGGAGGA GGGTAGCCAG 420CTGCTCTGCC TGGGCGAGCG CTGGTTGCTC GGCGGGTCGG GCGAGGCGCG CCTGGCCCTG 480CGTGGCGAGG ACTGGCCGCA GCTGGCGGCG CTCTGTCCGC CGCCCCGGCA AGCCTATGTG 540CCGCCCGGGC AGGCGGCGCC GCCGGGCGTC GAGGCCTGCC AGACGCTGGA GCAGCCGTGG 600CTCTGGCTGG CCGCGCAGAA GTCCGGCTGC AACCTGGCCC AGGGGCCTTT CGCCCGTCGC 660GAGCCTTCCG GCCAGTGGCA GCGCTGGCGG CCGCTGGCGG GGCTGCTCGG TCTCTGGCTG 720GTGCTGCAKT GGGGCTTCAA CCTTGCCCAN GGCTGGCAGC TGCAGCGCGA GGGTGAACGC 780TATGCCGTGG CCAACGAGGC GCTGTATCGC GAGCTGTTCC CCGAGGATCG CAAGGTGATC 840AACCTGCGTG CGCAGTTCGA CCAGCACCTG GCCGAGGCGG CTGGGAGCGG CCAGAGCCAG 900TTGCTGGCCC TGCTCGATCA GGCCGCCGCG GCCATCGGCG AAGGGGGGGC GCAGGTGCAG 960GTGGATCAGC TCGACTTCAA CGCCCAGCGT GGCGACCTGG CCTTCAACCT GCGTGCCAGC 1020GACTTCGCCG CGCTGGAAAG CCTGCGGGCG CGCCTGCAGG AGGCCGGCCT GGCGGTGGAC 1080ATGGGCTCGG CGAGCCGCGA GGACAACGGC GTCAGTGCGC GCCTGGTGAT CGGGGGTAAC 1140GGATGA 1146 381 amino acids amino acid single linear unknown 27Met Ser Leu Leu Thr Leu Phe Leu Pro Pro Gln Ala Cys Thr Glu Ala 1 5 10 15Ser Ala Asp Met Pro Val Trp Cys Val Glu Ser Asp Ser Cys Arg Gln 20 25 30Leu Pro Phe Ala Glu Ala Leu Pro Ala Asp Ala Arg Val Trp Arg Leu 35 40 45Val Leu Pro Val Glu Ala Val Thr Thr Cys Val Val Gln Leu Pro Thr 50 55 60Thr Lys Ala Arg Trp Leu Ala Lys Ala Leu Pro Phe Ala Val Glu Glu65 70 75 80Leu Leu Ala Glu Glu Val Glu Gln Phe His Leu Cys Val Gly Ser Ala 85 90 95Leu Val Asp Gly Arg His Arg Val His Ala Leu Arg Arg Glu Trp Leu 100 105 110Ala Gly Trp Leu Ala Leu Cys Gly Glu Arg Pro Pro Gln Trp Ile Glu 115 120 125Val Asp Ala Asp Leu Leu Pro Glu Glu Gly Ser Gln Leu Leu Cys Leu 130 135 140Gly Glu Arg Trp Leu Leu Gly Gly Ser Gly Glu Ala Arg Leu Ala Leu145 150 155 160Arg Gly Glu Asp Trp Pro Gln Leu Ala Ala Leu Cys Pro Pro Pro Arg 165 170 175Gln Ala Tyr Val Pro Pro Gly Gln Ala Ala Pro Pro Gly Val Glu Ala 180 185 190Cys Gln Thr Leu Glu Gln Pro Trp Leu Trp Leu Ala Ala Gln Lys Ser 195 200 205Gly Cys Asn Leu Ala Gln Gly Pro Phe Ala Arg Arg Glu Pro Ser Gly 210 215 220Gln Trp Gln Arg Trp Arg Pro Leu Ala Gly Leu Leu Gly Leu Trp Leu225 230 235 240Val Leu Xaa Trp Gly Phe Asn Leu Ala Xaa Gly Trp Gln Leu Gln Arg 245 250 255Glu Gly Glu Arg Tyr Ala Val Ala Asn Glu Ala Leu Tyr Arg Glu Leu 260 265 270Phe Pro Glu Asp Arg Lys Val Ile Asn Leu Arg Ala Gln Phe Asp Gln 275 280 285His Leu Ala Glu Ala Ala Gly Ser Gly Gln Ser Gln Leu Leu Ala Leu 290 295 300Leu Asp Gln Ala Ala Ala Ala Ile Gly Glu Gly Gly Ala Gln Val Gln305 310 315 320Val Asp Gln Leu Asp Phe Asn Ala Gln Arg Gly Asp Leu Ala Phe Asn 325 330 335Leu Arg Ala Ser Asp Phe Ala Ala Leu Glu Ser Leu Arg Ala Arg Leu 340 345 350Gln Glu Ala Gly Leu Ala Val Asp Met Gly Ser Ala Ser Arg Glu Asp 355 360 365Asn Gly Val Ser Ala Arg Leu Val Ile Gly Gly Asn Gly 370 375 380 4377 base pairs nucleic acid single linear unknown 28GAATTCGCCG CCGAGCTGGC CAAGCCGCTG GGCGCGGTGA CCGCACAGAA GGAAGTGGAG 60CGTGCCCTGC GCGACCTGCA CCTGCCCTTC GACGAGCGCC GTCCCTACGC CCTGCGCCGT 120CTGCGCGACC GCATCGAGGC CAATCTCTCC GGCCTGATGG GCCCCAGCGT GGCCCAGGAC 180ATGGTGGAAA CCTTCCTGCC CTACAAGGCC GGCAGCGAGG CCTATGTCAG CGAAGACATC 240CACTTCATCG AGAGTCGCCT GGAGGATTAC CAGTCGCGCC TCACCGGCCT GGCCGCCGAG 300CTCGACGCGC TGCGCCGCTT CCACCGCCAG ACCCTGCAGG AACTGCCGAT GGGCGTATGT 360TCGCTGGCCA AGGACCAGGA AGTGCTGATG TGGAACCGCG CCATGGAGGA ACTCACCGGC 420ATCAGCGCGC AGCAGGTGGT CGGCTCGCGC CTGCTCAGCC TGGAGCACCC CTGGCGCGAG 480CTGCTGCAGG ACTTCATCGC CCAGGACGAG GAGCACCTGC ACAAGCAGCA CCTGCAACTG 540GACGGCGAGG TGCGCTGGCT CAACCTGCAC AAGGCGGCCA TCGACGAACC GCTGGCGCCG 600GGCAACAGCG GCCTGGTGCT GCTGGTCGAG GACGTCACCG AGACCCGCGT GCTGGAAGAC 660CAGCTGGTGC ACTCCGAGCG TCTGGCCAGC ATCGGCCGCC TGGCCGCCGG GGTGGCCCAC 720GAGATCGGCA ATCCGGTCAC CGGCATCGCC TGCCTGGCGC AGAACCTGCG CGAGGAGCGC 780GAGGGCGACG AGGAGCTCGG CGAGATCAGC AACCAGATCC TCGACCAGAC CAAGCGCATC 840TCGCGCATCG TCCAGTCGCT GATGAACTTC GCCCACGCCG GCCAGCAGCA GCGCGCCGAA 900TACCCGGTGA GCCTGGCCGA AGTGGCGCAG GACGCCATCG GCCTGCTGTC GCTGAACCGC 960CATGGCACCG AAGTGCAGTT CTACAACCTG TGCGATCCCG AGCACCTGGC CAAGGGCGAC 1020CCGCAGCGCC TGGCCCAGGT GCTGATCAAC CTGCTGTCCA ACGCCCGCGA TGCCTCGCCG 1080GCCGGCGGTG CCATCCGCGT GCGTAGCGAG GCCGAGGAGC AGAGCGTGGT GCTGATCGTC 1140GAGGACGAGG GCACGGGCAT TCCGCAGGCG ATCATGGACC GCCTGTTCGA ACCCTTCTTC 1200ACCACCAAGG ACCCCGGCAA GGGCACCGGT TTGGGGCTCG CGCTGGTCTA TTCGATCGTG 1260GAAGAGCATT ATGGGCAGAT CACCATCGAC AGCCCGGCCG ATCCCGAGCA CCAGCGCGGA 1320ACCCGTTTCC GCGTGACCCT GCCGCGCTAT GTCGAAGCGA CGTCCACAGC GACCTGAGTA 1380GTGACCTAGA ACCGCCGAGG GGCCACAAGC CCGGCGGATT CGGAGACCGT CGAGAGAACA 1440CAATGCCGCA TATCCTCATC GTCGAAGACG AAACCATCAT CCGCTCCGCC CTGCGCCGCC 1500TGCTGGAACG CAACCAGTAC CAGGTCAGCG AGGCCGGTTC GGTTCAGGAG GCCCAGGAGC 1560GCTACAGCAT TCCGACCTTC GACCTGGTGG TCAGCGACCT GCGCCTGCCC GGCGCCCCCG 1620GCACCGAGCT GATCAAGCTG GCCGACGGCA CCCCGGTACT GATCATGACC AGCTATGCCA 1680GCCTGCGCTC GGCGGTGGAC TCGATGAAGA TGGGCGCGGT GGACTACATC GCCAAGCCCT 1740TCGATCACGA CGAGATGCTC CAGGCCGTGG CGCGTATCCT GCGCGATCAC CAGGAGGCCA 1800AGCGCAACCC GCCAAGCGAG GCGCCCAGCA AGTCCGCCGG CAAGGGCAAC GGCGCCACCG 1860CCGAGGGCGA GATCGGCATC ATCGGCTCCT GCGCCGCCAT GCAGGACCTT TACGGCAAGA 1920TCCGCAAGGT CGCTCCCACC GATTCCAACG TACTGATCCA GGGCGAGTCC GGCACCGGCA 1980AGGAGCTGGT CGCGCGTGCG CTGCACAACC TCTCGCGTCG CGCCAAGGCA CCGCTGATCT 2040CGGTGAACTG CGCGGCCATC CCCGAGACCC TGATCGAGTC CGAACTGTTC GGCCACGAGA 2100AAGGTGCCTT CACCGGCGCC AGCGCCGGCC GCGCCGGCCT GGTCGAAGCG GCCGACGGCG 2160GCACCCTGTT CCTCGACGAG ATCGGCGAGC TGCCGCTGGA GGCGCAGGCC CGCCTGCTGC 2220GCGTGCTGCA GGAGGGCGAG ATCCGTCGGG TCGGCTCGGT GCAGTCACAG AAGGTCGATG 2280TACGCCTGAT CGCCGCTACC CACCGCGACC TCAAGACGCT GGCCAAGACC GGCCAGTTCC 2340GCGAGGACCT CTACTACCGC CTGCACGTCA TCGCCCTCAA GCTGCCGCCA CTGCGCGAGC 2400GCGGCGCCGA CGTCAACGAG ATCGCCCGCG CCTTCCTCGT CCGCCAGTGC CAGCGCATGG 2460GCCGCGAGGA CCTGCGCTTC GCTCAGGATG CCGAGCAGGC GATCCGCCAC TACCCCTGGC 2520CGGGCAACGT GCGCGAGCTG GAGAATGCCA TCGAGCGCGC GGTGATCCTC TGCGAGGGCG 2580CGGAAATTTC CGCCGAGCTG CTGGGCATCG ACATCGAGCT GGACGACCTG GAGGACGGCG 2640ACTTCGGCGA ACAGCCACAG CAGACCGCGG CCAACCACGA ACCGACCGAG GACCTGTCGC 2700TGGAGGACTA CTTCCAGCAC TTCGTACTGG AGCACCAGGA TCACATGACC GAGACCGAAC 2760TGGCGCGCAA GCTCGGCATC AGCCGCAAGT GCCTGTGGGA GCGCCGTCAG CGCCTGGGCA 2820TTCCGCGGCG CAAGTCGGGC GCGGCGACCG GCTCCTGAAC GGGACGAACG GTGACAGGCC 2880TCGCCGCAAA AGGTTCCGCG CCTGTTACCC CGCACAAATA TCGCGTAACA AAAGCCGGGT 2940TCATCGGTAA CGGGAACCCG GCTTTTTTCT GCCCGCCGCC CGCACCAAAA AATCATAACT 3000CATTGAAAAA CAAGGAATTA CAAAAACTGG CACGGCTTCT GCTTTATCTC TGGCACAACA 3060ACAATAACAA CGCTCGAAAC CTCAACAATA AAAACAATAC AGAACGACTC CAGCACAACA 3120AAAACAACAA CGCGGAGGCG CAGCTAACTG ATTCTTTTGG AGAGGATTTG CCCTTGGGGT 3180TCGCCCCACA ACCAGGCCGA GAACAACAAA AACTGCACTA AAGCAGCGCC TGCACTGGTT 3240GGGTCATGGA ATGATCAAGG CAGCATCAGC ATCCAAAGCA ATCCGTTTGC TCCTGGTACC 3300CGATTTGGGC TACCTGAAAC GGGCCTACAA CAAAAACAAC AGGCCCGCAC AATAATAAAA 3360ACAAAGCACG CACCTATTTG GGGGGGAGCT TCGGCTCCCC CAGTAGCTTC ACCCCACCTC 3420GCGTTCCCCA GCCTGCCTTT TCCACCATCC CCCTTCCCGA TGCTAGAATC CGCGCCAATC 3480CTGCGGCGAT CTGCAATTGT GGCCGCCTAT TCCTGCAAAC AGTGCATCCC ATGCTGAAAA 3540AGCTGTTCAA GTCGTTTCGT TCACCTCTCA AGCGCCAAGC ACGCCCCCGC AGCACGCCGG 3600AAGTTCTCGG CCCGCGCCAG CATTCCCTGC AACGCAGCCA GTTCAGCCGC AATGCGGTAA 3660ACGTGGTGGA GCGCCTGCAG AACGCCGGCT ACCAGGCCTA TCTGGTCGGC GGCTGCGTAC 3720GCGACCTGCT GATCGGCGTG CAGCCCAAGG ACTTCGACGT GGCCACCAGC GCCACCCCCG 3780AGCAGGTGCG GGCCGAGTTT CGCAACGCCC GGGTGATCGG CCGCCGCTTC AAGCTGGCGC 3840ATGTGCATTT CGGCCGCGAG ATCATCGAGG TGGCGACCTT CCACAGCAAC CACCCGCAGG 3900GCGACGACGA GGAAGACAGC CACCAGTCGG CCCGTAACGA GAGCGGGCGC ATCCTGCGCG 3960ACAACGTCTA CGGCAGTCAG GAGAGCGATG CCCAGCGCCG CGACTTCACC ATCAACGCCC 4020TGTACTTCGA CGTCAGCGGC GAGCGCGTGC TGGACTATGC CCACGGCGTG CACGACATCC 4080GCAACCGCCT GATCCGCCTG ATCGGCGACC CCGAGCAGCG CTACCTGGAA GACCCGGTAC 4140GCATGCTGCG CGCCGTACGC TTCGCCGCCA AGCTGGACTT CGACATCGAG AAACACAGCG 4200CCGCGCCGAT CCGCCGCCTG GCGCCGATGC TGCGCGACAT CCCTGCCGCG CGCCTGTTCG 4260ACGAGGTGCT CAAGCTGTTC CTCGCCGGCT ACGCCGAGCG CACCTTCGAA CTGCTGCTCG 4320AGTACGACCT GTTCGCCCCG CTGTTCCCGG CCAGCGCCCG CGCCCTGGAG CGCGATC 4377 17612 base pairs nucleic acid single linear unknown 29GATCTCGAGG GCGTCGGCTT CGACACCCTG GCGGTGCGCG CCGGTCAGCA TCGCACGCCG 60GAGGGCGAGC ATGGCGAGGC CATGTTCCTC ACCTCCAGCT ATGTGTTCCG CAGCGCCGCC 120GACGCCGCCG CGCGCTTCGC CGGCGAGCAG CCGGGCAACG TCTACTCGCG CTACACCAAC 180CCGACCGTGC GCGCCTTCGA GGAGCGCATC GCCGCCCTGG AAGGCGCCGA GCAGGCGGTG 240GCCACCGCCT CCGGCATGGC CGCCATCCTG GCCATCGTCA TGAGCCTGTG CAGCGCCGGC 300GACCATGTGC TGGTGTCGCG CAGCGTGTTC GGCTCGACCA TCAGCCTGTT CGAGAAGTAC 360CTCAAGCGCT TCGGCATCGA GGTGGACTAC CCGCCGCTGG CCGATCTGGA CGCCTGGCAG 420GCAGCCTTCA AGCCCAACAC CAAGCTGCTG TTCGTCGAAT CGCCGTCCAA CCCGTTGGCC 480GAGCTGGTGG ACATAGGCGC CCTGGCCGAG ATCGCCCACG CCCGCGGCGC CCTGCTGGCG 540GTGGACAACT GCTTCTGCAC CCCGGCCCTG CAGCAGCCGC TGGCGCTGGG CGCCGATATG 600GTCATGCATT CGGCGACCAA GTTCATCGAT GGCCAGGGCC GCGGCCTGGG CGGCGTGGTG 660GCCGGGCGCC GTGCGCAGAT GGAGCAGGTG GTCGGCTTCC TGCGCACCGC CGGGCCGACC 720CTCAGCCCGT TCAACGCCTG GATGTTCCTC AAGGGCCTGG AGACCCTGCG TATCCGCATG 780CAGGCGCAGA GCGCCAGCGC CCTGGAACTG GCCCGCTGGT TGGAGACCCA GCCGGGCATC 840GACAGGGTCT ACTATGCCGG CCTGCCCAGC CACCCGCAGC ACGAGCTGGC CAAGCGGCAG 900CAGAGTGCCT TCGGCGCGGT GCTGAGCTTC GAGGTCAAGG GCGGCAAGGA GGCGGCCTGG 960CGTTTCATCG ATGCCACCCG GGTGATCTCC ATCACCACCA ACCTGGGCGA TACCAAGACC 1020ACCATCGCCC ATCCGGCGAC CACCTCCCAC GGTCGTCTGT CGCCGCAGGA GCGCGCCAGC 1080GCCGGTATCC GCGACAACCT GGTGCGTGTC GCCGTGGGCC TGGAAGACGT GGTCGACCTC 1140AAGGCCGACC TGGCCCGTGG CCTGGCCGCG CTCTGAGGAC GGGGGCCCCC GTTCCTGCCG 1200CGAAGGGCAG GGGCGGGGGC TTGCGGCGGG CCTTTGCGCG ATCAGCAGCT AGTCTTGGGG 1260AAACGTCCTA GCCCAGGAGC TACCCCATGA ACCTCATCCT TTTCCTGATC ATCGGCGCCG 1320TTGCCGGCTG GATCGCCGGC AAGTTGCTGC GTGGTGGCGG CTTCGGGCTG ATCGGCAACC 1380TGGTGGTGGG CATAGTGGGC GCGGTGATCG GCGGCCACCT GTTCAGCTAC CTGGGCGTGT 1440CCGCCGGTGG TGGGCTGATC GGCTCGCTGG TGACCGCGGT GATCGGTGCC CTGGTCCTGC 1500TGTTCATCGT CGGCCTGATC AAGAAGGCCC AGTAGCGCTG GCGGGACGCC GTCCCGCCGC 1560CCATCACTGG TCGCGCAGGT CCACGGCACC GGCGCCGGGT TTGTCGAACA GGCGCTCGGC 1620GCTGCCCGGC AGGCTGCTGT GGCCATCCTC GTCGGCACCC AGCACGCTGA TGTCGCTGTA 1680CTTCTTGCCC GACAGCGCGG CCATGCCGGC GCGGTCGCGG ACGATGGTCG GGCGCAGGAA 1740CACCATCAGG TTGCGCTTGA CGTGGGTGTC CTTGGTCGAG CGGAACAGCC GGCCGATCAG 1800CGGGATGTCA CCCAGCAGCG GCACCTTGGA GTCGGTGCTG GTGACGTCGT CCTGGATCAG 1860CCCTCCCAGC ACTATGACCT GGCCGTCGTC GGCCAGGATC ACGCTCTTGA TCGAGCGCTT 1920GTTGGTCACC AGGTCCACCG CCTGGGCATT GACCCCGGCG CTGGGGGCGA TGGAGGAGAT 1980CTCCTGCTCC ACTTCCAGGC GCAGGGTGGC GCCGTCGTTG ATGTGCGGGG TGACCTTGAG 2040GGTCACGCCG ATGTCCTCGC GCTCAATGGT GGTGAAGGGG TTGTTCGCCC CCGAGGCGTC 2100GGTGGTGTAG GAGCCGGTCT GGAAAGGCAC GTTCTGCCCG ACCAGGATTT CCGCCTCCTG 2160GTTGTCCAGG GTCAGCAGGC TGGGCGTGGA CAGCAGGTTG CTCTTGCTGT TGGCAGAGAG 2220GGCAGTGATC AGCGCGCCGA AGTTCTCGGT GCCGATGCCG ATGATGGCGC CGTCCGGCAG 2280GGTCAGGTCA TCGGGGATTT CCTCGTTCTG GATGGCCTTG AGCACGGTGC CCACCGATAG 2340CCCGGTATTG CCGAAGTTGA CCCCGCCGAG GCCGCCGGTG CCGCCGCGGG CATCCACCGC 2400CCACTGCACG CCGAGGGCGT CGCTGATGTC CCCGGAGATT TCCACGATGG CCGCCTCGAC 2460CATCACCTGG GCGCGCGGCA CGTCGAGGTT GCGCACGATT TCCTCGAGGG TCGCCACGGT 2520GTCCGGATCG GCCAGCAGGA CCAGGGCATT GAGGCTCTCG TCGGCGCGGA TCAGGATGTT 2580CTGCGGCTTG CTGCTGGCGG CTTCGCCACC ACCCTCCGCG GTCTTCAACC CCTCGGAGAT 2640GTCGCCCAGG GTCTCGGCCA GGCTCTTGGC GTCGCTGTGG CGTAGGCGAA TTACCCGCGC 2700ATTGGCCGAA CGGGTGCTGG GGATGTCCAG CGAGCGGGCC AGGTTGGCCA GGCGCTGGCG 2760GGCGGCCGGC GGGCCGAGGA GGATCAGGCG GTTGGTGCGG GCGTCGGCAA TCACCCGGGT 2820GCCGGCGCTG TTTTTCTCGT TGCGCATCAC CGCGTTGTTC AGTGCCTCGG CGGCGTCCAG 2880TACCCAGGCA TGCTGCAGGT TGATCACGTT GTAGTCGCCG CCGCCCTGGG CATCGAGCTC 2940GGCGATCAGT TCGCGGATGC GTTCGATATT NGCCCGGCGG TCGCTGATGA TCAGCGCGTT 3000GGAGGCGGCG ACCGCCGCCA GGTGGCCGTT CTGCGGCACC AGCGGGCGGA TCAGCGGGAT 3060CAGTTCGTTG ACCGAGGTGT GCTGCACCTG GATCAGCTCG GTCTGCACAT CGTCCGGCGC 3120GCTGCGGCTG CTGTTGGCGC CGCTACGCGC CTCGGTGACC GGCACGATGC GCGCCTGGTC 3180GCCCTGTGCC AGCACGCTGA AGCCATGGGT GCTCATCACC GAAAGGAACA GCTGGTAGAC 3240CTCCTCGAGG CCCAGCGGGG TCTTGGAGAT CACCGTGACC TGGCCCTTGA CCCGCGGATC 3300GACGACGAAG GTCTCGCCAG AGATCTGCGC CACCTGGTCG ATGAAGTCGC GGATATCGGC 3360GTCCTTCATG TTGATGGTCC AGGTCTCGGC GCCCTGGCTC ACCGCCACCG GCTCGGCGGC 3420ATGGACGAGC GGCAGCGGGG CGGCGAGGCA GCTCGCGGCC AGCAGCAGGG CGAGGGGCAG 3480GCGTTTGTGC GGCGGAATTC TGGAGTCGAT CATGGGCTGT CTTCGGCTTC CGGTATTTCG 3540GGCTGCGGGA TGTCGCCGCC TTCCATGCGT TGTTGAAGGG TCTGGATGCG CTCCTGCAGG 3600GCCTGGACGT CTTCGTCCTG CAGCTGTTCC AGTTGGCTGG CGGTGGGCTC CAGCGCCGAG 3660TAGGCCGGCG TCAGAGAGGG CTGGCGCACG GCGGGGAAGC GCAGGCTCTC CTCGACGCCG 3720CCGCGGTCGA GCACCACGTG GTCCTGATAG ACGGCCTGCA GGCGGGTGCT GACGTTGACC 3780GATTCGCCCA CGGCGATGCG CTTGGGTTTG TCGCCGGCGA CCTGGATGAT CGCCGTGGAG 3840CGCTTGGCGT CCGGGTTGAC GAAGCTGGCC AGCAGGGTCA TCTGCTGCCG GGTGGCGGGG 3900GCGGCCTGGT CGCCGCGCGG CCTGGCCGCG GGCGTGCCGA ACAGATGCTG CAGGCGCTGG 3960ATGGACAGCG GCTGGCGCTC GGCGATGCTC TCTGGGGCGG GCGGTGGCGC GGCCTCGCTG 4020CGCAGCAGGC GAAGGAAGTC GATGCTCTGC TTGCTCAGGC TGAGGGTGAT GAGCAGCACC 4080ACGAGCAGGC AGAGGCCGGT CACGCCGTGG CGCTGCAGCC AGGCGGGCAG GCGGGTGCGG 4140GTGCTACTCA AGGCATGGTT CCCCCGGTGT TCTTCTTATT CTGTGCGGAC GCTCTGCTCG 4200GCGTCTCGCA ATCCGGCCCG TACTCTGCGG GCGCAGGCAA CCTTAACGCA AGTCTCCTGT 4260CCATGGCGCA CCTGCTTCGT CTATCTGCGC GCTGGCGCAC TGTCCGCCGC TGCCGGAAGC 4320GTGAAACATT TCGAAACTTT CGGCGAACGA GTCGCTATCA TCGGCCCCAC GCGCTTCCCG 4380TTCAACAATA GCAATAAGCC AGACGGATTA CCGCCATGGA AGATCGCAAG CCGCCTGCCG 4440CGGCTCCCGT GGGGTTTGCG CGCGCGGAGC TGCTGGAGCT GCTCTGCCGC TGCGAGCAGT 4500TTCCCCTGAC CCTGCTGCTG GCGCCCGCCG GTTCCGGCAA GTCGACCCTG CTGGCCCAGT 4560GGCAGGCCAG CCGGCCCTTC GGCAGTGTGG TGCACTATCC ACTGCAGGCG CGTGACAACG 4620AGCCGGTACG CTTCTTCCGC CACCTGGCCG AAAGCATCCG CGCCCAGGTC GAGGACTTCG 4680ACCTGTCCTG GTTCAACCCC TTCGCCGCCG AGATGCACCA GGCGCCCGAG GTGCTCGGCG 4740AGTACCTGGC CGACGCCCTC AATCGCATCG AGAGCCGCCT CTACCTCGTC CTCGACGACT 4800TCCAGTGCAT CGGCCAGCCG ATCATCCTCG ACGTGCTCTC GGCCATGCTC GAACGCCTGG 4860CGGGCAACAC CCGGGTCATT CTGTCCGGGC GCAACCATCC GGGGTTCTCC CTCAGCCGCC 4920TGAAACTGGA CAACAAGCTG CTGTGCATCG ACCAGCACGA CATGCGCCTG TCGCCAGTGC 4980AGATCCAACA CCTCAATGCC TACCTGGGCG GTCCCGAGCT CAGCCCGGCC TATGTCGGCA 5040GCCTGATGGC CATGACCGAG GGCTGGATGG TCGGGGTGAA GATGGCCCTG ATGGCCCATG 5100CGCGCTTCGG CACCGAGGCC CTGCAGCGCT TCGGTGGCGG CCATCCGGAG ATAGTCGACT 5160ACTTCGGCCA TGTGGTGCTG AAGAAGCTGT CGCCGCAGCT GCACGACTTC CTGTTGTGCA 5220GCGCGATCTT CGAGCGCTTC GACGGCGAGC TATGCGACCG GGTGCTGGAT CGCAGCGGTT 5280CGGCCCTGCT GCTGGAGGAC CTGGCCGCGC GCGAGCTGTT CATGCTGCCG GTGGACGAGT 5340ATCCCGGCTG CTACCGCTAC CACGCCCTGT TGCACGATTT CCTCGCCCGG CGCCTGGCCG 5400TGCACAAGCC ACAGGAAGTG GCGCAACTGC ACCGGCGGGC GGCCCTGGCG CTGCAGCAGC 5460GTGGCGACCT GGAGCTGGCC CTGCAGCATG CCCAGCGCAG TGGCGACCGC GCGTTGTTCC 5520AAAGCATGCT GGGCGAGGCC TGCGAGCAAT GGGTGCGCAG CGGTCACTTC GCCGAGGTGC 5580TGAAGTGGCT GGAGCCGCTG AGCGAGGCGG AACTCTGCGN GCAGTCGCGC CTGCTGGTGC 5640TGATGACCTA TGCCCTGACC CTGTCGCGGC GTTTCCACCA GGCGCGCTAC TGCTTGGACG 5700AACTGGTGGC GCGCTGCACC GGTCAGCCGG GCCTGGAGGA GCCGACCCGC CAGCTGCTGG 5760CGCTCAACCT GGAGCTGTTC CAGCACGACC TGGCCTTCGA CCCCGGCCAG CGCTGGTCCG 5820ACCTGCTGGC CGCGGGCGTC GCCTCGGACA TCCGTGCCCT GGCGCTGAGC ATCCTCGCCT 5880ATCACCACCT GATGCACGGC CGCCTGGAGC AGTCGATCCA GCTGGCGCTG GAGGCCAAGG 5940CGCTGCTGGC CAGCACCGGC CAGCTGTTCC TGGAGAGCTA CGCCGACCTG ATCATCGCCC 6000TGTGCAACCG CAACGCCGGG CGCGCCACCA GCGCGCGCAA GGACGTCTGC CTGGATTACC 6060AGCGCACCGA GCGCTCCTCG CCGGCCTGGG TCAACCGTGC CACCGCCATG GTGGTGGCGC 6120TGTACGAGCA GAACCAGCTG GCCGCCGCCC AGCAGCTGTG CGAGGACCTG ATGGCCATGG 6180TCACGTCGTC CTCGGCCACC GAGACCATCG CCACCGTGCA CATCACCCTG TCGCGCCTGC 6240TCCACCGGCG CCAGTCCCAG GGCCGCGCCA CGCGCCTGCT GGAGCAGCTG TCGCGCATCC 6300TGCAACTGGG CAACTACGCC CGCTTCGCCA GCCAGGCGGC GCAGGAGAGC ATGCGCCAGG 6360CCTATCTCGA CGGGCGCCCG GCGGCGCTCG ACGCACTGGC CCAACGCCTG GGTATCGAGG 6420AGCGCCTGGC CGCCGGGGAG TGGGAGAGGG TGCGGCCCTA TGAAGAGTGC TGGGAACGCT 6480ACGGCCTGGC CGCCGTGTAC TGGCTGGTGA TGCGCGGCGC CCAGCCGCGC GCCTGCCGCA 6540TCCTCAAGGT GCTGGCGCAG GCGNTGNAGA ACAGCGAGAT GAAGGCCCGT GCGCTGGTGG 6600TGGAGGCCAA CCTGCTGGTG CTGAACGCCC CGCAGCTGGG GGCGGACGAG CAGGACAGGG 6660CCCTGCTGGC GCTGGTCGAG CGCTTCGGCA TCGTCAACAT CAACCGCTCG GTATTCGACG 6720AGGCGCCCGG CTTCGCCGAG GCGGTGTTCG GCCTGCTGCG CTCGGGCCGG CTGCAGGCGC 6780CGGAGGCCTA TCGCGAGGCC TATGCCGACT TCCTCCAGGG CACAGGCCAG GCGCCGCCGG 6840CGCTCCTGTC CGAGTCGCTG AAACAGCTTA CCGACAAGGA GGCGGCGATC TTCGCCTGCC 6900TGCTCAGGGG GCTGTCCAAC AGCGAGATCA GCGCCAGCAC CGGCATCGCC CTGTCCACCA 6960CCAAGTGGCA CCTGAAGAAC ATCTACTCGA AGCTGAGCCT CTCCGGGCGT ACCGAAGCCA 7020TCCTCGCCAT GCAGGCCCGC AACGGATAAT GCGCCATGCC CCTCCCCGGG GAGGGGGGAG 7080GGGCGCGCGC AACTGCTTAA TCTCCCGCCT GCCGGAAAAG CCGGCAAGCA ACCCCATTAG 7140TACAAGAAGA AATCGGGAGA TATCGCCATG TCTGTTTGGG TCACGTGGCC GGGCTTGGTC 7200AAGTTCGGCA CCCTGGGCAT CTATGCCGGC CTGATCACGC TCGCGCTTGA GCGCGACGTG 7260CTGTTCAAGA ACAACCTGTT CGACGTCGAC AACCTGCCCG CGGCCAACGC CAGCATCACC 7320TGTGATGCCC GCAGCCAGGT GGCGCGTACC GAGGACGGCA CCTGTAACAT CCTCGCCAAC 7380CCGGCCGAGG GCTCGGTGTA CCGCCGCTTC GGGCGCAACG TCGACCCCAG CGTGACCCAT 7440GGCGAGACCG AGGCCGACAC CCTGCTCAGT CCCAATCCGC GGGAGGTGAG TAACGTGCTG 7500ATGGCGCGTG GCGAGTTCAA GCCGGCGCCC AGCCTCAACT TCATCGCCGC CTCCTGGATC 7560CAGTTCATGG TGCATGACTG GGTCGAACAC GGCCCCAACG CCGAAGCCAA CCCGATCCAG 7620GTGCCGCTGC CGGCTGGCGA CGCGCTCGGC TCCGGCAGCC TGTCCGTGCG CCGCACCCAG 7680CCCGACCCGA CCCGTACCCC GGCCGAGGCC GGCAAGCCGG CCACCTACCG CAACCACAAC 7740ACCCACTGGT GGGATGGCTC GCAGTTGTAT GGCAGCAGCA AGGACATCAA CGACAAGGTG 7800CGCGCCTTCG AGGGTGGCAA GCTGAAGATC AATCCCGACG GTACCCTGCC GACCGAGTTC 7860CTCAGCGGCA AGCCGATCAC CGGCTTCAAC GAGAACTGGT GGGTTGGCCT GAGCATGCTG 7920CACCAGCTGT TCACTAAGGA GCACAACGCC ATCGCGGCGA TGCTCCAGCA GAAGTACCCG 7980GACAAGGACG ACCAGTGGCT GTACGACCAT GCGCGCCTGG TCAACTCCGC GCTGATGGCC 8040AAGATCCACA CCGTGGAATG GACCCCGGCG GTGATCGCCA ACCCGGTCAC CGAACGCGCC 8100ATGTATGCCA ACTGGTGGGG CCTGCTGGGT TCCGGTCCGG AGCGTGACAA GTACCAGGAA 8160GAGGCGCGCA TGCTGCAGGA GGACCTGGCC AGCTCCAACT CCTTCGTCCT GCGCATTCTC 8220GGCATCGACG GCAGCCAGGC CGGCAGTTCG GCCATCGACC ATGCCCTGGC CGGCATCGTC 8280GGCTCGACCA ACCCGAACAA CTACGGCGTG CCCTACACCC TGACCGAGGA GTTCGTCGCG 8340GTCTACCGCA TGCACCCGCT GATGCGCGAC AAGGTCGATG TCTACGACAT CGGCTCGAAC 8400ATCATCGCGC GCAGCGTGCC GCTGCAGGAG ACCCGCGATG CCGACGCCGA GGAGCTGCTG 8460GCGGACGAGA ATCCCGAGCG CCTGTGGTAC TCCTTCGGCA TCACCAACCC GGGCTCGCTG 8520ACCCTCAACA ACTACCCGAA CTTCCTGCGC AACCTGTCCA TGCCGCTGGT CGGCAACATC 8580GACCTGGCGA CCATCGACGT GCTGTGTGAC CGCGAGCGCG GGGTGCCGCG CTACAACGAG 8640TTCCGCCGCG AGATCGGCCT CAACCCGATC ACCAAGTTGG AGGACCTGAC CACCGACCCG 8700GCCACCCTGG CCAACCTCAA GCGCATCTAC GGCAACGACA TCGAGAAGAT TGACACCCTG 8760GTCGGCATGC TGGCCGAGAC CGTGCGTCCG GACGGCTTCG CCTTCGGCGA GACGGCCTTC 8820CAGATCTTCA TCATGAACGC CTCGCGGCGC CTGATGACCG ACCGCTTCTA TACCAAGGAC 8880TACCGCCCGG AGATCTACAC CGCCGAGGGC CTGGCCTGGG TCGAGAACAC CACCATGGTC 8940GACGTGCTCA AACGCCACAA TCCGCAGCTG GTCAACAGCC TGGTTGGCGT GGAAAACGCC 9000TTCAAACCCT GGGGCCTGAA CATCCCGGCC GACTACGAGA GCTGGCCGGG CAAGGCCAAG 9060CAGGACAACC TGTGGGTCAA CGGCGCCNTG CGCACCCAGT ACGCCGCAGG CCAGCTGCCG 9120GCCATTCCGC CGGTGGACGT CGGCGGCCTG ATCAGTTCGG TGCTGTGGAA GAAGGTGCAG 9180ACCAANTCCG ACGTGGCGCC GGCCGGCTAC GAGAAGGCCA TGCACCCGCA TGGCGTGATG 9240GCCAAGGTCA AGTTCACCGC CGTGCCGGGG CACCCCTACA CCGGCCTGTT CCAGGGTGCC 9300GACAGCGGCC TGCTGCGCCT GTCGGTGGCC GGCGACCCGG CAACCAACGG CTTCCAGCCG 9360GGTCTGGCGT GGAAGGCCTT CGTCGACGGC AAGCCGTCGC AGAACGTCTC CGCGCTCTAC 9420ACCCTGAGCG GGCAGGGCAG CAACCACAAC TTCTTCGCCA ACGAGCTGTC GCAGTTCGTC 9480CTGCCGGAGA CCAACGATAC CCTGGGCACC ACGCTGCTGT TCTCGCTGGT CAGCCTCAAG 9540CCGACCTTGC TGCGCGTGGA CGACATGGCC GAAGTGACCC AGACCGGCCA GGCCGTGACT 9600TCGGTCAAGG CGCCGACGCA GATCTACTTC GTGCCCAAGC CGGAGCTGCG CAGCCTGTTC 9660TCCAGTGCGG CGCATGACTT CCGCAGCGAC CTGACGAGCC TCACCGCCGG CACCAAGCTG 9720TACGACGTCT ACGCTACCTC GATGGAGATC AAGACCTCGA TCCTGCCGTC GACCAATCGT 9780AGCTACGCCC AGCAACGGCG CAACAGCGCG GTGAAGATCG GCGAGATGGA GCTGACCTCG 9840CCGTTCATCG CCTCGGCCTT CGGCGACAAC GGGGTGTTCT TCAAGCACCA GCGTCACGAA 9900GACAAATAAG GGTCATCCCT TGCTGAACAG CCCCGGCCCG TGCCGGGGCT TTTTTGTGCA 9960CGCCTTACGT CCATCACACT TCTGCGCCAG GCTGTGCTGC CGCCTGCAAA ATCGGCACTG 10020CAGTTTTTGC GCAAATCCGT TAACTTGGCG CCTCGGCCAT GCCATAAAAA CAACAAGAAC 10080AACAGCAAGA TGGATCTTCT GTTCGGGGAA CGCATCCGCC CATGTCCACC GATACCCACG 10140CCGCCCTGAC GGCTCCCGCA AGCCCCGCCT TGCGCCCGCT GCCCTTCGCC TTCGCCAAAC 10200GCCACGGCGT GCTGCTGCGC GAGCCCTTCG GCCAGGTCCA GCTGCAGGTG CGCCGCGGTG 10260CCAGCCTGGC CGCCGTGCAG GAGGCCCAGC GCTTCGCCGG CCGCGTGCTG CCGCTGCACT 10320GGCTGGAGCC CGAGGCCTTC GAGCAGGAGC TGGCCCTGGC CTACCAGCGC GACTCCTCCG 10380AGGTGCGGCA GATGGCCGAG GGCATGGGTG CCGAACTTGA CCTAGCCAGC CTGGCCGAAC 10440TCACTCCCGA ATCCGGCGAC CTGCTGGAGC AGGAAGATGA CGCGCCGATC ATCCGCCTGA 10500TCAACGCCAT CCTCAGCGAG GCGATCAAGG CCGGCGCCTC CGACATCCAC CTGGAAACCT 10560TCGAGAAACG CCTGGTGGTG CGCTTTCGCG TCGACGGCAT CCTCCGCGAA GTGATCGAAC 10620CGCGCCGCGA GCTGGCGGCG CTGCTGGTCT CGCGGGTCAA GGTCATGGCG CGCCTGGACA 10680TCGCCGAGAA GCGCGTACCG CAGGACGGCC GTATTTCGCT CAAGGTCGGC GGTCGCGAGG 10740TGGATATCCG CGTCTCCACC CTGCCGTCGG CCAACGGCGA GCGGGTGGTG CTGCGTCTGC 10800TCGACAAGCA GGCCGGGCGC CTGTCGCTCA CGCATCTGGG CATGAGCGAG CGCGACCGCC 10860GCCTGCTCGA CGACAACCTG CGCAAGCCGC ACGGCATCAT CCTAGTCACC GGCCCCACCG 10920GCTCGGGCAA GACCACCACC CTGTACGCCG GCCTGGTCAC CCTCAACGAC CGCTCGCGCA 10980ATATCCTCAC GGTGGAAGAC CCGATCGAGT ACTACCTGGA AGGCATCGGC CAGACCCAGG 11040TCAACCCGCG GGTGGACATG ACCTTCGCCC GCGGCCTGCG CGCCATCCTG CGCCAGGACC 11100CGGACGTGGT GATGGTCGGC GAGATCCGCG ACCAGGAGAC CGCCGACATC GCCGTGCAGG 11160CCTCGCTCAC CGGCCACCTG GTGCTCTCCA CCCTGCACAC CAACAGCGCC GTCGGCGCCG 11220TCACCCGCCT GGTCGACATG GGCGTCGAGC CCTTCCTGCT GTCGTCGTCC CTGCTCGGCG 11280TGCTGGCCCA GCGCCTGGTG CGCGTGCTCT GCGTGCACTG CCGCGAGGCG CGCCCGGCTG 11340ACGCGGCCGA GTGCGGCCTG CTCGGCCTCG ACCCGCACAG CCAGCCCCTG ATCTACCACG 11400CCAAGGGCTG CCCGGAGTGC CACCAGCAGG GCTACCGCGG CCGTACTGGC ATCTACGAGC 11460TGGTGATCTT CGACGACCAG ATGCGCACCC TGGTGCACAA CGGCGCCGGT GAGCAGGAGC 11520TGATTCGCCA CGCCCGCAGC CTCGGCCCGA GCATCCGCGA CGATGGCCGG CGCAAGGTGC 11580TGGAAGGGGT GACCAGCCTG GAAGAAGTGT TGCGCGTGAC CCGGGAAGAC TGATGGCCGC 11640CTTCGAATAC ATCGCCCTGG ATGCCAGGGG CCGCCAGCAG AAGGGCGTGC TGGAGGGCGA 11700CAGCGCCCGC CAGGTGCGCC AGCTGCTGCG CGACAAACAG TTGTCGCCGC TGCAGGTCGA 11760GCCGGTACAG CGCAGGGAGC AGGCCGAGGC TGGTGGCTTC AGCCTGCGCC GTGGCCTGTC 11820GGCGCGCGAC CTGGCGCTGG TCACCCGTCA GCTGGCGACC CTGATCGGCG CCGCGCTGCC 11880CATCGAGGAA GCGCTGCGCG CCGCCGCCGC GCAGTCGCGC CAGCCGCGCA TCCAGTCGAT 11940GCTGTTGGCG GTGCGCGCCA AGGTGCTCGA GGGCCACAGC CTGGCCAAGG CCCTGGCCTC 12000CTACCCGGCG GCCTTCCCCG AGCTGTACCG CGCCACGGTG GCGGCCGGCG AGCATGCGGG 12060GCACCTGGCG CCGGTGCTGG AGCAGCTGGC CGACTACACC GAGCAGCGCC AGCAGTCGCG 12120GCAGAAGATC CAGATGGCGC TGCTCTACCC GGTGATCCTG ATGCTCGCTT CGCTGGGCAT 12180CGTCGGTTTT CTGCTCGGCT ACGTGGTGCC GGATGTGGTG CGGGTGTTCG TCGACTCCGG 12240GCAGACCCTG CCGGCGCTGA CCCGCGGGCT GATTTTCCTC AGCGAGCTGG TCAAGTCCTG 12300GGGCGCCCTG GCCATCGTCC TGGCGGTGCT CGGCGTGCTC GCCTTTCGCC GCGCCTTGCG 12360CAGCGAGGAT CTGCGCCGGC GCTGGCATGC CTTCCTGCTG CGCGTGCCGC TGGTCGGTGG 12420GCTGATCGCC GCCACCGAGA CGGCACGCTT CGCCTCGACC CTGGCCATCC TGGTGCGCAG 12480CGGCGTGCCA CTGGTGGAGG CGCTGGCCAT CGGCGCCGAG GTGGTGTCCA ACCTGATCAT 12540CCGCAGCGAC GTGGCCAACG CCACCCAGCG CGTGCGCGAG GGCGGCAGCC TGTCGCGCGC 12600GCTGGAAGCC AGCCGGCAGT TTCCGCCGAT GATGCTGCAC ATGATCGCCA GCGGCGAGCG 12660TTCCGGCGAG CTGGACCAGA TGCTGGCGCG CACGGCGCGC AACCAGGAAA ACGACCTGGC 12720GGCCACCATC GGCCTGCTGG TGGGGCTGTT CGAGCCGTTC ATGCTGGTAT TCATGGGCGC 12780GGTGGTGCTG GTGATCGTGC TGGCCATCCT GCTGCCGATT CTTTCTCTGA ACCAACTGGT 12840GGGTTGATAG CGATGTACAA ACAGAAAGGC TTCACGCTGA TCGAAATCAT GGTGGTGGTG 12900GTCATCCTCG GCATTCTCGC TGCCCTGGTG GTGCCGCAGG TGATGGGCCG CCCGGACCAG 12960GCCAAGGTCA CCGCGGCGCA GAACGACATC CGCGCCATCG GCGCCGCGCT GGACATGTAC 13020AAGCTGGACA ACCAGAACTA CCCGAGCACC CAGCAGGGCC TGGAGGCCCT GGTGAAGAAA 13080CCCACCGGCA CGCCGGCGGC GAAGAACTGG AACGCCGAGG GCTACCTGAA GAAGCTGCCG 13140GTCGACCCCT GGGGCAACCA GTACCTGTAC CTGTCGCCGG GCACCCGCGG CAAGATCGAC 13200CTGTATTCGC TGGGCGCCGA CGGCCAGGAA GGCGGCGAGG GGACCGACGC CGACATCGGC 13260AACTGGGATC TCTGACTCGC AATGCAGCGG GGGCGCGGTT TCACTCTGAT CGAGCTGCTG 13320GTGGTGCTGG TGCTGCTGGG CGTGCTCACC GGCCTCGCCG TGCTCGGCAG CGGGATCGCC 13380AGCAGCCCCG CGCGCAAGCT GGCGGACGAG GCCGAGCGCC TGCAGTCGCT GCTGCGGGTG 13440CTGCTCGACG AGGCGGTGCT GGACAACCGC GAGTATGGCG TACGCTTCGA CGCCCGGAGC 13500TACCGGGTGC TGCGCTTCGA GCCGCGCACG GCGCGCTGGG AGCCGCTCGA CGAGCGCGTG 13560CACGAGCTGC CGGAGTGGCT CGAGCTGGAG ATCGAGGTCG ACGAGCAGAG TGTCGGGCTG 13620CCCGCCGCCC GTGGCGAGCA GGACAAAGCC GCGGCCAAGG CGCCACAGCT GCTGCTGCTC 13680TCCAGTGGCG AGCTGACCCC CTTCGCCCTG CGCCTGTCCG CCGGCCGCGA GCGCGGCGCG 13740CCGGTGCTGA CGCTGGCCAG CGACGGCTTC GCCGAGCCCG AGCTGCAGCA GGAAAAGTCC 13800CGATGAAGCG CGGCCGCGGC TTCACCCTGC TCGAGGTGCT GGTGGCCCTG GCGATCTTCG 13860CCGTGGTCGC CGCCAGCGTG CTCAGCGCCA GCGCTCGCTC GCTGAAGACC GCCGCGCGCC 13920TGGAGGACAA GACCTTCGCC ACCTGGCTGG CGGACAACCG CCTGCAGGAG CTGCAGCTGG 13980CCGACGTGCC GCCGGGCGAG GGCCGCGAGC AGGGCGAGGA GAGCTACGCC GGGCGGCGCT 14040GGCTGTGGCA GAGCGAGGTG CAGGCCACCA GCGAGCCGGA GATGCTGCGT GTCACCGTAC 14100GGGTGGCGCT GCGGCCGGAG CGCGGGCTGC AGGGCAAGAT CGAAGACCAT GCCCTGGTGA 14160CCCTGAGTGG CTTCGTCGGG GTCGAGCCAT GAGGCAGCGC GGCTTCACCC TGCTGGAAGT 14220GCTGATCGCC ATCGCCATCT TCGCCCTGCT GGCCATGGCC ACCTACCGCA TGCTCGACAG 14280CGTGCTGCAG ACCGATCGTG GCCAGCGCCA GCAGGAGCAG CGTCTGCGCG AGCTGACGCG 14340GGCCATGGCA GCTTTCGAAC GCGACCTGCT GCAGGTGCGC CTGCGTCCGG TGCGCGACCC 14400GCTGGGCGAC CTGCTGCCAG CCCTGCGCGG CAGCAGTGGC CGCGACACCC AGCTGGAGTT 14460CACCCGCAGC GGCTGGCGCA ACCCGCTCGG CCAGCCGCGC GCCACCCTAC AGCGGGTGCG 14520CTGGCAGCTC GAAGGCGAGC GCTGGCAGCG CGCTTACTGG ACGGTGCTGG ACCAGGCCCA 14580GGACAGCCAG CCGCGGGTGC AGCAGGCGCT GGATGGCGTG CGCCGCTTCG ACTTGCGCTT 14640TCTCGACCAG GAGGGGCGCT GGCTGCAGGA CTGGCCGCCG GCCAACAGTG CTGCCGACGA 14700GGCCCTGACC CAGCTGCCGC GTGCCGTCGA GCTGGTCGTC GAGCACCGCC ATTACGGTGA 14760ACTGCGCCGT CTCTGGCGCT TGCCCGAGAT GCCGCAGCAG GAACAGATCA CGCCGCCCGG 14820GGGCGAGCAG GGCGGTGAGC TGCTGCCGGA AGAGCCGGAG CCCGAGGCAT GAGCCGGCAG 14880CGCGGCGTGG CACTGATCAC CGTGCTGCTG GTGGTGGCGC TGGTGACCGT GGTCTGCGCG 14940GCCCTGCTGC TGCGCCAGCA GCTGGCCATC CGCAGCACCG GCAACCAGCT GCTGGTGCGC 15000CAGGCCCAGT ACTACGCCGA AGGCGGCGAG CTGCTGGCCA AGGCCCTGCT GCGTCGCGAC 15060CTGGCCGCCG ACCAGGTCGA TCATCCCGGC GAGCCCTGGG CCAACCCCGG CCTGCGCTTC 15120CCCCTGGATG AGGGCGGCGA GCTGCGCCTG CGCATCGAGG ACCTGGCCGG ACGTTTCAAC 15180CTCAACAGCC TGGCCGCCGG TGGTGAGGCC GGTGAGTTGG CGCTGCTGCG CCTGCGGCGC 15240CTGCTGCAGC TGCTGCAGCT GACCCCGGCC TATGCCGAGC GCCTGCAGGA CTGGCTCGAC 15300GGCGATCAGG AGGCCAGCGG CATGGCCGGC GCCGAGGATG ACCAGTACCT GCTGCAGAAA 15360CCGCCCTACC GTACCGGCCC CGGGCGCATT GCCGAGGTGT CGGAGCTGCG CCTGCTGCTG 15420GGCATGAGCG AGGCCGACTA CCGCCGCCTG GCCCCCTTCG TCAGCGCCCT GCCGAGCCAG 15480GTCGAGCTGA ACATCAACAC CGCCAGCGCC CTGGTGCTGG CTTGCCTGGG CGAGGGCATN 15540CCCGAGGCGG TGCTCGAGGC CGCCATCGAN GGTCGCGGCC GCAGCGGCTA TCGCGAGCCC 15600GCTGCCTTCG TCCAGCANCT TGCCAGCTAC GGCGTCAGCC CGCAGGGGCT GGGCATCGCC 15660AGCCAGTATT TCCGTGTCAC CACCGAGGTG CTGCTGGGTG AGCGGCGCCA GGTGCTGGCC 15720AGTTATCTGC AACGTGGTAA TGATGGGCGC GTCCGCCTGA TGGCGCGCGA TCTGGGGCAG 15780GAGGGCCTGG CGCCCCCACC CGTCGAGGAG TCCGAGAAAT GAGTCTGCTC ACCCTGTTTC 15840TGCCGCCCCA GGCCTGCACC GAGGCGAGCG CCGACATGCC GGTGTGGTGC GTCGAGAGCG 15900ACAGCTGCCG TCAGCTGCCC TTCGCCGAGG CCTTGCCGGC CGACGCGCGG GTCTGGCGCT 15960TGGTGCTGCC GGTGGAGGCG GTGACCACCT GTGTCGTGCA GTTGCCGACC ACCAAGGCAC 16020GCTGGCTGGC CAAGGCCCTG CCGTTCGCCG TCGAGGAGCT GCTGGCCGAG GAGGTGGAGC 16080AGTTTCACCT GTGCGTCGGT AGCGCGCTGG TCGATGGTCG TCATCGTGTT CATGCCCTGC 16140GCCGCGAGTG GCTGGCCGGC TGGCTGGCGC TGTGCGGCGA GCGGCCGCCG CAGTGGATCG 16200AGGTGGACGC CGACCTGTTG CCGGAGGAGG GTAGCCAGCT GCTCTGCCTG GGCGAGCGCT 16260GGTTGCTCGG CGGGTCGGGC GAGGCGCGCC TGGCCCTGCG TGGCGAGGAC TGGCCGCAGC 16320TGGCGGCGCT CTGTCCGCCG CCCCGGCAAG CCTATGTGCC GCCCGGGCAG GCGGCGCCGC 16380CGGGCGTCGA GGCCTGCCAG ACGCTGGAGC AGCCGTGGCT CTGGCTGGCC GCGCAGAAGT 16440CCGGCTGCAA CCTGGCCCAG GGGCCTTTCG CCCGTCGCGA GCCTTCCGGC CAGTGGCAGC 16500GCTGGCGGCC GCTGGCGGGG CTGCTCGGTC TCTGGCTGGT GCTGCAKTGG GGCTTCAACC 16560TTGCCCANGG CTGGCAGCTG CAGCGCGAGG GTGAACGCTA TGCCGTGGCC AACGAGGCGC 16620TGTATCGCGA GCTGTTCCCC GAGGATCGCA AGGTGATCAA CCTGCGTGCG CAGTTCGACC 16680AGCACCTGGC CGAGGCGGCT GGGAGCGGCC AGAGCCAGTT GCTGGCCCTG CTCGATCAGG 16740CCGCCGCGGC CATCGGCGAA GGGGGGGCGC AGGTGCAGGT GGATCAGCTC GACTTCAACG 16800CCCAGCGTGG CGACCTGGCC TTCAACCTGC GTGCCAGCGA CTTCGCCGCG CTGGAAAGCC 16860TGCGGGCGCG CCTGCAGGAG GCCGGCCTGG CGGTGGACAT GGGCTCGGCG AGCCGCGAGG 16920ACAACGGCGT CAGTGCGCGC CTGGTGATCG GGGGTAACGG ATGAACGGCC TGCTCATGCA 16980ATGGCAAGCG CGCCTGGCGC AGAACCCTTT GATGCTGCGC TGGCAGGGCC TGCCGCCACG 17040CGACCGGCTG GCCCTGGGCC TGCTCGCTGC CTTCCTGTTG CTGGTGCTGC TGTACCTGTT 17100GCTGTGGCGG CCGGTCAGCC AGAACCTGGA GCGGGCGCGC GGCTTCCTGC AGCAGCAGCG 17160TACGCTGCAC GCCTACCTGC AGGAGCATGC ACCGCAGGTG CGGGCACGGC AGGTCGCACC 17220GCAGGCCAGT ATCGAGCCTG CCGCGCTGCA GGGGTTGGTG ACCGCCAGTG CCGCCAGCCA 17280GGGGCTGAAT GTCGAGCGTC TGGACAACCA GGGTGATGGT GGCCTGCAGG TGAGCCTGCA 17340GCCGGTCGAG TTCGCCCGTC TGCTGCAGTG GCTGGTGAGC CTGCAGGAGC AGGGCGTGCG 17400CGTCGAAGAG GCCGGTCTGG AACGTGCCGA CAAGGGGCTG GTGAGCAGCC GCCTGCTGCT 17460GCGTGCCGGT TGAGCCCGGC TGCACCAGGC GAGTGCGTCG GCACTCGCGC GGAGCATCTG 17520GAAAACCCGT CCGCGAAGAA AAATTCAAGC AGGGTGTTGA CTTAGCTATG ACCTCTNCGT 17580CAATTGCGCG CCTCGCANGC TAACGGCTGG AT 17612 2634 base pairs nucleic acid single linear unknown 30ATGGAAGATC GCAAGCCGCC TGCCGCGGCT CCCGTGGGGT TTGCGCGCGC GGAGCTGCTG 60GAGCTGCTCT GCCGCTGCGA GCAGTTTCCC CTGACCCTGC TGCTGGCGCC CGCCGGTTCC 120GGCAAGTCGA CCCTGCTGGC CCAGTGGCAG GCCAGCCGGC CCTTCGGCAG TGTGGTGCAC 180TATCCACTGC AGGCGCGTGA CAACGAGCCG GTACGCTTCT TCCGCCACCT GGCCGAAAGC 240ATCCGCGCCC AGGTCGAGGA CTTCGACCTG TCCTGGTTCA ACCCCTTCGC CGCCGAGATG 300CACCAGGCGC CCGAGGTGCT CGGCGAGTAC CTGGCCGACG CCCTCAATCG CATCGAGAGC 360CGCCTCTACC TCGTCCTCGA CGACTTCCAG TGCATCGGCC AGCCGATCAT CCTCGACGTG 420CTCTCGGCCA TGCTCGAACG CCTGGCGGGC AACACCCGGG TCATTCTGTC CGGGCGCAAC 480CATCCGGGGT TCTCCCTCAG CCGCCTGAAA CTGGACAACA AGCTGCTGTG CATCGACCAG 540CACGACATGC GCCTGTCGCC AGTGCAGATC CAACACCTCA ATGCCTACCT GGGCGGTCCC 600GAGCTCAGCC CGGCCTATGT CGGCAGCCTG ATGGCCATGA CCGAGGGCTG GATGGTCGGG 660GTGAAGATGG CCCTGATGGC CCATGCGCGC TTCGGCACCG AGGCCCTGCA GCGCTTCGGT 720GGCGGCCATC CGGAGATAGT CGACTACTTC GGCCATGTGG TGCTGAAGAA GCTGTCGCCG 780CAGCTGCACG ACTTCCTGTT GTGCAGCGCG ATCTTCGAGC GCTTCGACGG CGAGCTATGC 840GACCGGGTGC TGGATCGCAG CGGTTCGGCC CTGCTGCTGG AGGACCTGGC CGCGCGCGAG 900CTGTTCATGC TGCCGGTGGA CGAGTATCCC GGCTGCTACC GCTACCACGC CCTGTTGCAC 960GATTTCCTCG CCCGGCGCCT GGCCGTGCAC AAGCCACAGG AAGTGGCGCA ACTGCACCGG 1020CGGGCGGCCC TGGCGCTGCA GCAGCGTGGC GACCTGGAGC TGGCCCTGCA GCATGCCCAG 1080CGCAGTGGCG ACCGCGCGTT GTTCCAAAGC ATGCTGGGCG AGGCCTGCGA GCAATGGGTG 1140CGCAGCGGTC ACTTCGCCGA GGTGCTGAAG TGGCTGGAGC CGCTGAGCGA GGCGGAACTC 1200TGCGNGCAGT CGCGCCTGCT GGTGCTGATG ACCTATGCCC TGACCCTGTC GCGGCGTTTC 1260CACCAGGCGC GCTACTGCTT GGACGAACTG GTGGCGCGCT GCACCGGTCA GCCGGGCCTG 1320GAGGAGCCGA CCCGCCAGCT GCTGGCGCTC AACCTGGAGC TGTTCCAGCA CGACCTGGCC 1380TTCGACCCCG GCCAGCGCTG GTCCGACCTG CTGGCCGCGG GCGTCGCCTC GGACATCCGT 1440GCCCTGGCGC TGAGCATCCT CGCCTATCAC CACCTGATGC ACGGCCGCCT GGAGCAGTCG 1500ATCCAGCTGG CGCTGGAGGC CAAGGCGCTG CTGGCCAGCA CCGGCCAGCT GTTCCTGGAG 1560AGCTACGCCG ACCTGATCAT CGCCCTGTGC AACCGCAACG CCGGGCGCGC CACCAGCGCG 1620CGCAAGGACG TCTGCCTGGA TTACCAGCGC ACCGAGCGCT CCTCGCCGGC CTGGGTCAAC 1680CGTGCCACCG CCATGGTGGT GGCGCTGTAC GAGCAGAACC AGCTGGCCGC CGCCCAGCAG 1740CTGTGCGAGG ACCTGATGGC CATGGTCACG TCGTCCTCGG CCACCGAGAC CATCGCCACC 1800GTGCACATCA CCCTGTCGCG CCTGCTCCAC CGGCGCCAGT CCCAGGGCCG CGCCACGCGC 1860CTGCTGGAGC AGCTGTCGCG CATCCTGCAA CTGGGCAACT ACGCCCGCTT CGCCAGCCAG 1920GCGGCGCAGG AGAGCATGCG CCAGGCCTAT CTCGACGGGC GCCCGGCGGC GCTCGACGCA 1980CTGGCCCAAC GCCTGGGTAT CGAGGAGCGC CTGGCCGCCG GGGAGTGGGA GAGGGTGCGG 2040CCCTATGAAG AGTGCTGGGA ACGCTACGGC CTGGCCGCCG TGTACTGGCT GGTGATGCGC 2100GGCGCCCAGC CGCGCGCCTG CCGCATCCTC AAGGTGCTGG CGCAGGCGNT GNAGAACAGC 2160GAGATGAAGG CCCGTGCGCT GGTGGTGGAG GCCAACCTGC TGGTGCTGAA CGCCCCGCAG 2220CTGGGGGCGG ACGAGCAGGA CAGGGCCCTG CTGGCGCTGG TCGAGCGCTT CGGCATCGTC 2280AACATCAACC GCTCGGTATT CGACGAGGCG CCCGGCTTCG CCGAGGCGGT GTTCGGCCTG 2340CTGCGCTCGG GCCGGCTGCA GGCGCCGGAG GCCTATCGCG AGGCCTATGC CGACTTCCTC 2400CAGGGCACAG GCCAGGCGCC GCCGGCGCTC CTGTCCGAGT CGCTGAAACA GCTTACCGAC 2460AAGGAGGCGG CGATCTTCGC CTGCCTGCTC AGGGGGCTGT CCAACAGCGA GATCAGCGCC 2520AGCACCGGCA TCGCCCTGTC CACCACCAAG TGGCACCTGA AGAACATCTA CTCGAAGCTG 2580AGCCTCTCCG GGCGTACCGA AGCCATCCTC GCCATGCAGG CCCGCAACGG ATAA 2634 877 amino acids amino acid single linear unknown 31Met Glu Asp Arg Lys Pro Pro Ala Ala Ala Pro Val Gly Phe Ala Arg 1 5 10 15Ala Glu Leu Leu Glu Leu Leu Cys Arg Cys Glu Gln Phe Pro Leu Thr 20 25 30Leu Leu Leu Ala Pro Ala Gly Ser Gly Lys Ser Thr Leu Leu Ala Gln 35 40 45Trp Gln Ala Ser Arg Pro Phe Gly Ser Val Val His Tyr Pro Leu Gln 50 55 60Ala Arg Asp Asn Glu Pro Val Arg Phe Phe Arg His Leu Ala Glu Ser65 70 75 80Ile Arg Ala Gln Val Glu Asp Phe Asp Leu Ser Trp Phe Asn Pro Phe 85 90 95Ala Ala Glu Met His Gln Ala Pro Glu Val Leu Gly Glu Tyr Leu Ala 100 105 110Asp Ala Leu Asn Arg Ile Glu Ser Arg Leu Tyr Leu Val Leu Asp Asp 115 120 125Phe Gln Cys Ile Gly Gln Pro Ile Ile Leu Asp Val Leu Ser Ala Met 130 135 140Leu Glu Arg Leu Ala Gly Asn Thr Arg Val Ile Leu Ser Gly Arg Asn145 150 155 160His Pro Gly Phe Ser Leu Ser Arg Leu Lys Leu Asp Asn Lys Leu Leu 165 170 175Cys Ile Asp Gln His Asp Met Arg Leu Ser Pro Val Gln Ile Gln His 180 185 190Leu Asn Ala Tyr Leu Gly Gly Pro Glu Leu Ser Pro Ala Tyr Val Gly 195 200 205Ser Leu Met Ala Met Thr Glu Gly Trp Met Val Gly Val Lys Met Ala 210 215 220Leu Met Ala His Ala Arg Phe Gly Thr Glu Ala Leu Gln Arg Phe Gly225 230 235 240Gly Gly His Pro Glu Ile Val Asp Tyr Phe Gly His Val Val Leu Lys 245 250 255Lys Leu Ser Pro Gln Leu His Asp Phe Leu Leu Cys Ser Ala Ile Phe 260 265 270Glu Arg Phe Asp Gly Glu Leu Cys Asp Arg Val Leu Asp Arg Ser Gly 275 280 285Ser Ala Leu Leu Leu Glu Asp Leu Ala Ala Arg Glu Leu Phe Met Leu 290 295 300Pro Val Asp Glu Tyr Pro Gly Cys Tyr Arg Tyr His Ala Leu Leu His305 310 315 320Asp Phe Leu Ala Arg Arg Leu Ala Val His Lys Pro Gln Glu Val Ala 325 330 335Gln Leu His Arg Arg Ala Ala Leu Ala Leu Gln Gln Arg Gly Asp Leu 340 345 350Glu Leu Ala Leu Gln His Ala Gln Arg Ser Gly Asp Arg Ala Leu Phe 355 360 365Gln Ser Met Leu Gly Glu Ala Cys Glu Gln Trp Val Arg Ser Gly His 370 375 380Phe Ala Glu Val Leu Lys Trp Leu Glu Pro Leu Ser Glu Ala Glu Leu385 390 395 400Cys Xaa Gln Ser Arg Leu Leu Val Leu Met Thr Tyr Ala Leu Thr Leu 405 410 415Ser Arg Arg Phe His Gln Ala Arg Tyr Cys Leu Asp Glu Leu Val Ala 420 425 430Arg Cys Thr Gly Gln Pro Gly Leu Glu Glu Pro Thr Arg Gln Leu Leu 435 440 445Ala Leu Asn Leu Glu Leu Phe Gln His Asp Leu Ala Phe Asp Pro Gly 450 455 460Gln Arg Trp Ser Asp Leu Leu Ala Ala Gly Val Ala Ser Asp Ile Arg465 470 475 480Ala Leu Ala Leu Ser Ile Leu Ala Tyr His His Leu Met His Gly Arg 485 490 495Leu Glu Gln Ser Ile Gln Leu Ala Leu Glu Ala Lys Ala Leu Leu Ala 500 505 510Ser Thr Gly Gln Leu Phe Leu Glu Ser Tyr Ala Asp Leu Ile Ile Ala 515 520 525Leu Cys Asn Arg Asn Ala Gly Arg Ala Thr Ser Ala Arg Lys Asp Val 530 535 540Cys Leu Asp Tyr Gln Arg Thr Glu Arg Ser Ser Pro Ala Trp Val Asn545 550 555 560Arg Ala Thr Ala Met Val Val Ala Leu Tyr Glu Gln Asn Gln Leu Ala 565 570 575Ala Ala Gln Gln Leu Cys Glu Asp Leu Met Ala Met Val Thr Ser Ser 580 585 590Ser Ala Thr Glu Thr Ile Ala Thr Val His Ile Thr Leu Ser Arg Leu 595 600 605Leu His Arg Arg Gln Ser Gln Gly Arg Ala Thr Arg Leu Leu Glu Gln 610 615 620Leu Ser Arg Ile Leu Gln Leu Gly Asn Tyr Ala Arg Phe Ala Ser Gln625 630 635 640Ala Ala Gln Glu Ser Met Arg Gln Ala Tyr Leu Asp Gly Arg Pro Ala 645 650 655Ala Leu Asp Ala Leu Ala Gln Arg Leu Gly Ile Glu Glu Arg Leu Ala 660 665 670Ala Gly Glu Trp Glu Arg Val Arg Pro Tyr Glu Glu Cys Trp Glu Arg 675 680 685Tyr Gly Leu Ala Ala Val Tyr Trp Leu Val Met Arg Gly Ala Gln Pro 690 695 700Arg Ala Cys Arg Ile Leu Lys Val Leu Ala Gln Ala Xaa Xaa Asn Ser705 710 715 720Glu Met Lys Ala Arg Ala Leu Val Val Glu Ala Asn Leu Leu Val Leu 725 730 735Asn Ala Pro Gln Leu Gly Ala Asp Glu Gln Asp Arg Ala Leu Leu Ala 740 745 750Leu Val Glu Arg Phe Gly Ile Val Asn Ile Asn Arg Ser Val Phe Asp 755 760 765Glu Ala Pro Gly Phe Ala Glu Ala Val Phe Gly Leu Leu Arg Ser Gly 770 775 780Arg Leu Gln Ala Pro Glu Ala Tyr Arg Glu Ala Tyr Ala Asp Phe Leu785 790 795 800Gln Gly Thr Gly Gln Ala Pro Pro Ala Leu Leu Ser Glu Ser Leu Lys 805 810 815Gln Leu Thr Asp Lys Glu Ala Ala Ile Phe Ala Cys Leu Leu Arg Gly 820 825 830Leu Ser Asn Ser Glu Ile Ser Ala Ser Thr Gly Ile Ala Leu Ser Thr 835 840 845Thr Lys Trp His Leu Lys Asn Ile Tyr Ser Lys Leu Ser Leu Ser Gly 850 855 860Arg Thr Glu Ala Ile Leu Ala Met Gln Ala Arg Asn Gly865 870 875 513 base pairs nucleic acid single linear unknown 32ATGAACGGCC TGCTCATGCA ATGGCAAGCG CGCCTGGCGC AGAACCCTTT GATGCTGCGC 60TGGCAGGGCC TGCCGCCACG CGACCGGCTG GCCCTGGGCC TGCTCGCTGC CTTCCTGTTG 120CTGGTGCTGC TGTACCTGTT GCTGTGGCGG CCGGTCAGCC AGAACCTGGA GCGGGCGCGC 180GGCTTCCTGC AGCAGCAGCG TACGCTGCAC GCCTACCTGC AGGAGCATGC ACCGCAGGTG 240CGGGCACGGC AGGTCGCACC GCAGGCCAGT ATCGAGCCTG CCGCGCTGCA GGGGTTGGTG 300ACCGCCAGTG CCGCCAGCCA GGGGCTGAAT GTCGAGCGTC TGGACAACCA GGGTGATGGT 360GGCCTGCAGG TGAGCCTGCA GCCGGTCGAG TTCGCCCGTC TGCTGCAGTG GCTGGTGAGC 420CTGCAGGAGC AGGGCGTGCG CGTCGAAGAG GCCGGTCTGG AACGTGCCGA CAAGGGGCTG 480GTGAGCAGCC GCCTGCTGCT GCGTGCCGGT TGA 513 170 amino acids amino acid single linear unknown 33Met Asn Gly Leu Leu Met Gln Trp Gln Ala Arg Leu Ala Gln Asn Pro 1 5 10 15Leu Met Leu Arg Trp Gln Gly Leu Pro Pro Arg Asp Arg Leu Ala Leu 20 25 30Gly Leu Leu Ala Ala Phe Leu Leu Leu Val Leu Leu Tyr Leu Leu Leu 35 40 45Trp Arg Pro Val Ser Gln Asn Leu Glu Arg Ala Arg Gly Phe Leu Gln 50 55 60Gln Gln Arg Thr Leu His Ala Tyr Leu Gln Glu His Ala Pro Gln Val65 70 75 80Arg Ala Arg Gln Val Ala Pro Gln Ala Ser Ile Glu Pro Ala Ala Leu 85 90 95Gln Gly Leu Val Thr Ala Ser Ala Ala Ser Gln Gly Leu Asn Val Glu 100 105 110Arg Leu Asp Asn Gln Gly Asp Gly Gly Leu Gln Val Ser Leu Gln Pro 115 120 125Val Glu Phe Ala Arg Leu Leu Gln Trp Leu Val Ser Leu Gln Glu Gln 130 135 140Gly Val Arg Val Glu Glu Ala Gly Leu Glu Arg Ala Asp Lys Gly Leu145 150 155 160Val Ser Ser Arg Leu Leu Leu Arg Ala Gly 165 170 1176 base pairs nucleic acid single linear unknown 34GATCTCGAGG GCGTCGGCTT CGACACCCTG GCGGTGCGCG CCGGTCAGCA TCGCACGCCG 60GAGGGCGAGC ATGGCGAGGC CATGTTCCTC ACCTCCAGCT ATGTGTTCCG CAGCGCCGCC 120GACGCCGCCG CGCGCTTCGC CGGCGAGCAG CCGGGCAACG TCTACTCGCG CTACACCAAC 180CCGACCGTGC GCGCCTTCGA GGAGCGCATC GCCGCCCTGG AAGGCGCCGA GCAGGCGGTG 240GCCACCGCCT CCGGCATGGC CGCCATCCTG GCCATCGTCA TGAGCCTGTG CAGCGCCGGC 300GACCATGTGC TGGTGTCGCG CAGCGTGTTC GGCTCGACCA TCAGCCTGTT CGAGAAGTAC 360CTCAAGCGCT TCGGCATCGA GGTGGACTAC CCGCCGCTGG CCGATCTGGA CGCCTGGCAG 420GCAGCCTTCA AGCCCAACAC CAAGCTGCTG TTCGTCGAAT CGCCGTCCAA CCCGTTGGCC 480GAGCTGGTGG ACATAGGCGC CCTGGCCGAG ATCGCCCACG CCCGCGGCGC CCTGCTGGCG 540GTGGACAACT GCTTCTGCAC CCCGGCCCTG CAGCAGCCGC TGGCGCTGGG CGCCGATATG 600GTCATGCATT CGGCGACCAA GTTCATCGAT GGCCAGGGCC GCGGCCTGGG CGGCGTGGTG 660GCCGGGCGCC GTGCGCAGAT GGAGCAGGTG GTCGGCTTCC TGCGCACCGC CGGGCCGACC 720CTCAGCCCGT TCAACGCCTG GATGTTCCTC AAGGGCCTGG AGACCCTGCG TATCCGCATG 780CAGGCGCAGA GCGCCAGCGC CCTGGAACTG GCCCGCTGGT TGGAGACCCA GCCGGGCATC 840GACAGGGTCT ACTATGCCGG CCTGCCCAGC CACCCGCAGC ACGAGCTGGC CAAGCGGCAG 900CAGAGTGCCT TCGGCGCGGT GCTGAGCTTC GAGGTCAAGG GCGGCAAGGA GGCGGCCTGG 960CGTTTCATCG ATGCCACCCG GGTGATCTCC ATCACCACCA ACCTGGGCGA TACCAAGACC 1020ACCATCGCCC ATCCGGCGAC CACCTCCCAC GGTCGTCTGT CGCCGCAGGA GCGCGCCAGC 1080GCCGGTATCC GCGACAACCT GGTGCGTGTC GCCGTGGGCC TGGAAGACGT GGTCGACCTC 1140AAGGCCGACC TGGCCCGTGG CCTGGCCGCG CTCTGA 1176 392 amino acids amino acid single linear unknown 35Tyr Asp Leu Glu Gly Val Gly Phe Asp Thr Leu Ala Val Arg Ala Gly 1 5 10 15Gln His Arg Thr Pro Glu Gly Glu His Gly Glu Ala Met Phe Leu Thr 20 25 30Ser Ser Tyr Val Phe Arg Ser Ala Ala Asp Ala Ala Ala Arg Phe Ala 35 40 45Gly Glu Gln Pro Gly Asn Val Tyr Ser Arg Tyr Thr Asn Pro Thr Val 50 55 60Arg Ala Phe Glu Glu Arg Ile Ala Ala Leu Glu Gly Ala Glu Gln Ala65 70 75 80Val Ala Thr Ala Ser Gly Met Ala Ala Ile Leu Ala Ile Val Met Ser 85 90 95Leu Cys Ser Ala Gly Asp His Val Leu Val Ser Arg Ser Val Phe Gly 100 105 110Ser Thr Ile Ser Leu Phe Glu Lys Tyr Leu Lys Arg Phe Gly Ile Glu 115 120 125Val Asp Tyr Pro Pro Leu Ala Asp Leu Asp Ala Trp Gln Ala Ala Phe 130 135 140Lys Pro Asn Thr Lys Leu Leu Phe Val Glu Ser Pro Ser Asn Pro Leu145 150 155 160Ala Glu Leu Val Asp Ile Gly Ala Leu Ala Glu Ile Ala His Ala Arg 165 170 175Gly Ala Leu Leu Ala Val Asp Asn Cys Phe Cys Thr Pro Ala Leu Gln 180 185 190Gln Pro Leu Ala Leu Gly Ala Asp Met Val Met His Ser Ala Thr Lys 195 200 205Phe Ile Asp Gly Gln Gly Arg Gly Leu Gly Gly Val Val Ala Gly Arg 210 215 220Arg Ala Gln Met Glu Gln Val Val Gly Phe Leu Arg Thr Ala Gly Pro225 230 235 240Thr Leu Ser Pro Phe Asn Ala Trp Met Phe Leu Lys Gly Leu Glu Thr 245 250 255Leu Arg Ile Arg Met Gln Ala Gln Ser Ala Ser Ala Leu Glu Leu Ala 260 265 270Arg Trp Leu Glu Thr Gln Pro Gly Ile Asp Arg Val Tyr Tyr Ala Gly 275 280 285Leu Pro Ser His Pro Gln His Glu Leu Ala Lys Arg Gln Gln Ser Ala 290 295 300Phe Gly Ala Val Leu Ser Phe Glu Val Lys Gly Gly Lys Glu Ala Ala305 310 315 320Trp Arg Phe Ile Asp Ala Thr Arg Val Ile Ser Ile Thr Thr Asn Leu 325 330 335Gly Asp Thr Lys Thr Thr Ile Ala His Pro Ala Thr Thr Ser His Gly 340 345 350Arg Leu Ser Pro Gln Glu Arg Ala Ser Ala Gly Ile Arg Asp Asn Leu 355 360 365Val Arg Val Ala Val Gly Leu Glu Asp Val Val Asp Leu Lys Ala Asp 370 375 380Leu Ala Arg Gly Leu Ala Ala Leu385 390 847 base pairs nucleic acid single linear unknown 36ATGCTGAAAA AGCTGTTCAA GTCGTTTCGT TCACCTCTCA AGCGCCAAGC ACGCCCCCGC 60AGCACGCCGG AAGTTCTCGG CCCGCGCCAG CATTCCCTGC AACGCAGCCA GTTCAGCCGC 120AATGCGGTAA ACGTGGTGGA GCGCCTGCAG AACGCCGGCT ACCAGGCCTA TCTGGTCGGC 180GGCTGCGTAC GCGACCTGCT GATCGGCGTG CAGCCCAAGG ACTTCGACGT GGCCACCAGC 240GCCACCCCCG AGCAGGTGCG GGCCGAGTTT CGCAACGCCC GGGTGATCGG CCGCCGCTTC 300AAGCTGGCGC ATGTGCATTT CGGCCGCGAG ATCATCGAGG TGGCGACCTT CCACAGCAAC 360CACCCGCAGG GCGACGACGA GGAAGACAGC CACCAGTCGG CCCGTAACGA GAGCGGGCGC 420ATCCTGCGCG ACAACGTCTA CGGCAGTCAG GAGAGCGATG CCCAGCGCCG CGACTTCACC 480ATCAACGCCC TGTACTTCGA CGTCAGCGGC GAGCGCGTGC TGGACTATGC CCACGGCGTG 540CACGACATCC GCAACCGCCT GATCCGCCTG ATCGGCGACC CCGAGCAGCG CTACCTGGAA 600GACCCGGTAC GCATGCTGCG CGCCGTACGC TTCGCCGCCA AGCTGGACTT CGACATCGAG 660AAACACAGCG CCGCGCCGAT CCGCCGCCTG GCGCCGATGC TGCGCGACAT CCCTGCCGCG 720CGCCTGTTCG ACGAGGTGCT CAAGCTGTTC CTCGCCGGCT ACGCCGAGCG CACCTTCGAA 780CTGCTGCTCG AGTACGACCT GTTCGCCCCG CTGTTCCCGG CCAGCGCCCG CGCCCTGGAG 840CGCGATC 847 282 amino acids amino acid single linear unknown 37Met Leu Lys Lys Leu Phe Lys Ser Phe Arg Ser Pro Leu Lys Arg Gln 1 5 10 15Ala Arg Pro Arg Ser Thr Pro Glu Val Leu Gly Pro Arg Gln His Ser 20 25 30Leu Gln Arg Ser Gln Phe Ser Arg Asn Ala Val Asn Val Val Glu Arg 35 40 45Leu Gln Asn Ala Gly Tyr Gln Ala Tyr Leu Val Gly Gly Cys Val Arg 50 55 60Asp Leu Leu Ile Gly Val Gln Pro Lys Asp Phe Asp Val Ala Thr Ser65 70 75 80Ala Thr Pro Glu Gln Val Arg Ala Glu Phe Arg Asn Ala Arg Val Ile 85 90 95Gly Arg Arg Phe Lys Leu Ala His Val His Phe Gly Arg Glu Ile Ile 100 105 110Glu Val Ala Thr Phe His Ser Asn His Pro Gln Gly Asp Asp Glu Glu 115 120 125Asp Ser His Gln Ser Ala Arg Asn Glu Ser Gly Arg Ile Leu Arg Asp 130 135 140Asn Val Tyr Gly Ser Gln Glu Ser Asp Ala Gln Arg Arg Asp Phe Thr145 150 155 160Ile Asn Ala Leu Tyr Phe Asp Val Ser Gly Glu Arg Val Leu Asp Tyr 165 170 175Ala His Gly Val His Asp Ile Arg Asn Arg Leu Ile Arg Leu Ile Gly 180 185 190Asp Pro Glu Gln Arg Tyr Leu Glu Asp Pro Val Arg Met Leu Arg Ala 195 200 205Val Arg Phe Ala Ala Lys Leu Asp Phe Asp Ile Glu Lys His Ser Ala 210 215 220Ala Pro Ile Arg Arg Leu Ala Pro Met Leu Arg Asp Ile Pro Ala Ala225 230 235 240Arg Leu Phe Asp Glu Val Leu Lys Leu Phe Leu Ala Gly Tyr Ala Glu 245 250 255Arg Thr Phe Glu Leu Leu Leu Glu Tyr Asp Leu Phe Ala Pro Leu Phe 260 265 270Pro Ala Ser Ala Arg Ala Leu Glu Arg Asp 275 280

Claims

1. An isolated nucleic acid encoding a kinase from a Pseudomonad that can regulate the expression of a lipase.

2. The isolated nucleic acid of claim 1 that hybridizes under stringent conditions to nucleic acid having the sequence as shown in SEQ ID NO: 1.

3. The isolated nucleic acid of claim 2 that has the sequence as shown in SEQ ID NO: 1.

4. An expression vector comprising the isolated nucleic acid of claim 2.

5. An expression vector comprising the isolated nucleic acid of claim 3.

6. A host cell comprising the expression vector of claim 4.

7. The host cell of claim 6 that is a Bacterium.

8. The host cell of claim 6 that is a Pseudomonad.

9. The host cell of claim 6 that further comprises nucleic acid encoding a desired protein.

10. The host cell of claim 9 where the desired protein is an enzyme.

11. The host cell of claim 10 wherein the enzyme includes esterases, hydrolases, lipases, isomerases, mutases, transferases, kinases, and phosphatases.