Patent Application
20230242423
The present invention relates to a plant powder extract and a water-purifying agent containing the extract, both used for purification of water such as industrial wastewater.
In recent years, large amounts of waste liquids including environmentally hazardous substances such as metal ions and fluorine ions as inorganic ions have been generated in the processes for producing various products in plants.
Meanwhile, regulations on effluence of such inorganic ions have been becoming gradually strict. In order to comply with the effluent control, an inorganic ion removing method that can effectively remove inorganic ions from wastewater including the inorganic ions and can be carried out as easily and inexpensively as possible is demanded.
Hitherto, as the method for removing impurity ions from, for example, wastewater from plants, for example, a flocculating precipitation method, an ion-exchange method, a method for adsorption to an adsorbent such as activated charcoal, an electrosorption method, and a magnetic adsorption method have been proposed.
For example, as the flocculating precipitation method, there has been proposed a method of performing a step of adding a base to wastewater in which heavy metal ions are dissolved, to make the wastewater basic, insolubilize at least part of the heavy metal ions, and form a suspended solid matter, a step of adding an inorganic flocculant to the wastewater to make the suspended solid matter flocculate and settle, a step of adding a polymeric flocculant to the wastewater to turn the suspended solid matter to a macrofloc, and an adsorbing step of passing the wastewater through an adsorption layer containing a cation exchanger formed of a leafy vegetable such as mulukhiya and Japanese mustard spinach (see, e.g., PTL 1).
There has also been proposed a flocculating method of flocculating and separating particles in a suspension by means of mixed use or combined use of a flocculant containing at least any one of mulukhiya, a dried product of mulukhiya, and an extract of mulukhiya with a polymeric flocculant (see, e.g., PTL 2).
There have also been proposed a water-purifying agent formed of a granulated substance containing a mixture of a plant powder and a polymeric flocculant, and a water-purifying method using the water-purifying agent (see, e.g., PTL 3).
However, although it hitherto has been known that plant powders can be used for water purification of wastewater, what contribute to water purification, or the specific contributing components have not been elucidated. Hence, there is a matter of study left in use of plant powders for water purification of wastewater.
The present invention has an object to identify effective components in plant powders that contribute to water purification and provide a water-purifying agent that can exhibit an excellent water-purifying performance to wastewater efficiently even in a small amount and infallibly.
Means for solving the above problems are as follows.
<1> An extract, being formed of
<2> The extract according to <1>,
wherein the ethanol-undissolved component and the ethanol-dissolved component of the fractionated component 1 exhibit the peaks near 1,700 (cm-1) and near 1,600 (cm -1) in the FT-IR measurement.
<3> The extract according to <1> or <2>,
wherein the fractionated component 1 contains a substance having a weight average molecular weight (Mw) of 300,000 or greater by 50% or greater.
<4> The extract according to any one of <1> to <3>,
wherein the plant powder is a powder of Corchorus olitorius.
<5> An extract, being formed of
<6> The extract according to <5>,
wherein the ethanol-undissolved component and the ethanol-dissolved component of the fractionated component 2 exhibit main peaks in a range of from 1,590 (cm-1) through 1,630 (cm-1) in the FT-IR measurement.
<7> The extract according to <5> or <6>,
wherein the ethanol-undissolved component and the ethanol-dissolved component of the fractionated component 2 exhibit peaks of 1,8-diazacyclotetradecane-2,7-dione in a GC-MS measurement.
<8> The extract according to any one of <5> to <7>,
wherein the fractionated component 2 contains a substance having a weight average molecular weight (Mw) of from 200 through 2,500 by 90% or greater.
<9> The extract according to any one of <5> to <8>,
wherein the fractionated component 2 is a water-soluble chitosan.
<10> The extract according to any one of <5> to <9>,
wherein the plant powder is a powder of Corchorus olitorius.
<11> A water-purifying agent, including:
the extract according to any one of <1> to <4>.
<12> A water-purifying agent, including:
<13> A water-purifying agent, including:
the extract according to any one of <5> to <10>.
<14> A water-purifying agent, including:
<15> The water-purifying agent according to <14>,
wherein the ethanol-undissolved component and the ethanol dissolved component of the fractionated component 1 exhibit the peaks near 1,700 (cm-1) and near 1,600 (cm-1) in the FT-IR measurement.
<16> The water-purifying agent according to <14> or <15>,
wherein the fractionated component 1 contains a substance having a weight average molecular weight (Mw) of 300,000 or greater by 50% or greater.
<17> The water-purifying agent according to any one of <14> to <16>,
wherein the plant powder is a powder of Corchorus olitorius.
<18> A water-purifying agent, including:
<19> The water-purifying agent according to <18>,
wherein the ethanol-undissolved component and the ethanol-dissolved component of the fractionated component 2 exhibit main peaks in a range of from 1,590 (cm-1) through 1,630 (cm-1) in the FT-IR measurement.
<20> The water-purifying agent according to <18> or <19>,
wherein the ethanol-undissolved component and the ethanol-dissolved component of the fractionated component 2 exhibit peaks of 1,8-diazacyclotetradecane-2,7-dione in a GC-MS measurement.
<21> The water-purifying agent according to any one of <18> to <20>,
wherein the fractionated component 2 contains a substance having a weight average molecular weight (Mw) of from 200 through 2,500 by 90% or greater.
<22> The water-purifying agent according to any one of <18> to <21>,
wherein the fractionated component 2 is a water-soluble chitosan.
<23> The water-purifying agent according to any one of <18> to <22>,
wherein the plant powder is a powder of Corchorus olitorius.
<24> The water-purifying agent according to any one of <14> to <17>,
wherein the water-purifying agent is the water-purifying agent according to any one of <18> to <23>.
<25> The water-purifying agent according to any one of <11> to <24>, including:
a polymeric flocculant.
<26> The water-purifying agent according to <25>,
wherein the polymeric flocculant is polyacrylamide.
<27> A wastewater treatment method, including:
feeding the water-purifying agent according to any one of <11> to <26> to wastewater, to remove an inorganic unnecessary substance in the wastewater.
<28> The wastewater treatment method according to <27>,
wherein the wastewater is wastewater including the inorganic unnecessary substance containing at least any one selected from the group consisting of nickel, fluorine, iron, copper, zinc, chromium, arsenic, cadmium, tin, and lead.
The present invention can provide a water-purifying agent that can exhibit an excellent water-purifying performance to wastewater efficiently even in a small amount and infallibly.
As a result of earnest studies into the water-purifying function of a plant powder, the present inventors have found effective components of a plant powder that contribute to water purification.
It has been confirmed that among water extracts of the plant powder, extracted components, which are formed of a fractionated component 1 (may also be referred to as component 1 in the present invention) having a fractionation molecular weight of 12,000 or greater and a fractionated component 2 (may also be referred to as component 2 in the present invention) having a fractionation molecular weight of less than 3,400 illustrated in
Here, the plant is not particularly limited and any plant that contains the component 1 and the component 2 in effective amounts respectively may be used. Preferable examples of the plant include Corchorus olitorius and mulukhiya.
Particularly, as Corchorus olitorius, for example, Corchorus olitorius produced in Nansha City of China, or “intermediate jute No. 4” under nationally identified hemp 2013, “intermediate jute No. 3” under varieties identification of registration No. 1209006 in Anhui province, “intermediate jute No. 1” under XPD005-2005, and “intermediate kenaf” under varieties identification of registration No. 1209001 in Anhui province, which are identification numbers in Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, can be suitably used.
Above all, the “intermediate jute No. 4”, the “intermediate jute No. 3”, and the “intermediate kenaf” are more preferable, and the “intermediate jute No. 4” is particularly preferable.
The identification number of the “intermediate jute No. 3” is indicated in
The “intermediate jute No. 4” has the following properties.
Agricultural product type: Jute
The fractionated component 1 can be extracted according to a method illustrated in
An ethanol-undissolved component (denoted by component A in
As plotted in
The component A was also measured by a gas chromatography mass spectrometry (GC-MS) method. The result of the measurement is plotted in
As plotted in
Attributions of the peaks (A1) to (A20) in
The symbol “O” affixed to the above signs denotes a peak of a fragment attributable to cellulose.
The symbol “Δ” affixed to the above signs denotes a peak of a fragment attributable to gluten (plant protein).
An ethanol-dissolved component (denoted by component B in
As plotted in
The component B was also measured by GC-MS. The result of the measurement is plotted in
As plotted in
Attributions of the peaks (B1) to (B16) in
The symbol “O” affixed to the above signs denotes a peak of a fragment attributable to cellulose.
The symbol “□” affixed to the above signs denotes a peak of a fragment attributable to cellulose acetate.
The symbol “Δ” affixed to the above signs denotes a peak of a fragment attributable to gluten (plant protein).
From the results of the FT-IR measurements and the GC-MS measurements of the component A and the component B, the component 1 is considered to be formed of uronic acid or carboxylic acid that has a structure similar to galacturonic acid. Hence, the component 1 is considered to have inorganic ions adsorb thereto and exhibit an excellent effect in water purification.
The component 1 was also measured by gel permeation chromatograph (GPC). The result of the measurement is plotted in
From the result of
An experiment of a water-purifying action was performed using the extract formed of the component 1. The result is plotted in
In
From the result of
The fractionated component 2 can be extracted according to the method illustrated in
An ethanol-undissolved component (denoted by component G in
As plotted in
The component G was also measured by GC-MS. The result of the measurement is plotted in
As plotted in
Attributions of the peaks (C1) to (C13) in
The symbol “X” affixed to the above signs denotes a peak of a fragment attributable to polysaccharide such as chitin and chitosan.
The symbol “Δ” affixed to the above signs denotes a peak of a fragment attributable to gluten (plant protein).
An ethanol-dissolved component (denoted by component H in
As plotted in
The component H was also measured by GC-MS. The result of the measurement is plotted in
As plotted in
Attributions of the peaks (D1) to (D17) in
The symbol “O” affixed to the above sign denotes a peak of a fragment attributable to cellulose.
The symbol “X” affixed to the above signs denotes a peak of a fragment attributable to polysaccharide such as chitin and chitosan.
The symbol “Δ” affixed to the above signs denotes a peak of a fragment attributable to gluten (plant protein).
The result of a microscopic infrared spectroscopy (microscopic IR) measurement in which the extract formed of the component 2 was compared with chitosan is plotted in
From the results of the FT-IR measurements and the GC-MS measurements of the component G and the component H and the result of
In the present invention, water solubility means solubility of 50% by mass of greater in water.
The component 2 was also measured by GPC. The result of the measurement is plotted in
From the result of
An experiment of a water-purifying action was performed using the extract formed of the component 2. The result is plotted in
In
From the results of
Moreover, as plotted in
The water-purifying agent of the present invention contains a plant powder.
As the first mode of the water-purifying agent of the present invention, it is preferable that the plant powder contain the component 1 or the component 2, or both thereof, which is/are (Extract of plant powder) described above.
This is because an effective water-purifying action can be exhibited efficiently even if the amount of addition is small.
As the second mode of the water-purifying agent of the present invention, it is preferable that the water-purifying agent contain a plant powder that contains extracted component(s) formed of the component 1 or the component 2, or both thereof in predetermined effective amount(s).
The water-purifying agent of the present invention contains the component 1 or the component 2, or both thereof extracted by the production method described above.
Particularly, it is preferable that the water-purifying agent of the present invention contain both of the component 1 and the component 2 extracted by the production method described above. As plotted in
The water-purifying agent of the present invention contains a plant powder containing extracted component(s) formed of the component 1 or the component 2, or both thereof.
Here, the component 1 is contained in the plant powder in an amount of 0.5% by mass or greater, more preferably in an amount of 0.7% by mass, and yet more preferably in an amount of 0.9% by mass as will be demonstrated by Examples.
The component 2 is contained in the plant powder in an amount of 0.05% by mass or greater, and more preferably in an amount of 0.07% by mass.
According to the flowchart illustrated in
Next, the component 1 and the component 2 were extracted from a dried product of Corchorus olitorius including only leaves. The results as the yields of the respective components are presented below (Table 2 below).
As can be seen from Table 1 below and Table 2 below, the yields of the component 1 and the component 2 vary depending on the plant powder, which is the raw material. Hence, it is preferable to appropriately adjust the contents of the component 1 and the component 2 to desired ranges respectively, by varying the ratio among leaves, stalks, and roots of the plant.
In addition to the powder of the plant, the water-purifying agent may contain additives such as a polymeric flocculant, a filler, a thickener, a colorant, and a thixotropy imparting agent as other additives.
The polymeric flocculant is not particularly limited so long as the polymeric flocculant exhibits an effect of removing the inorganic unnecessary substance in wastewater like the powder of the plant described above. Examples of the polymeric flocculant include polyacrylamide (PAM), a salt obtained by partially hydrolyzing polyacrylamide, sodium alginate, sodium polyacrylate, and CMC sodium salt. Among these polymeric flocculants, polyacrylamide is preferable for use. As the polyacrylamide, for example, commercially available products FLOPAN AN 956, FLOPAN AN 995SH, FA 920SH, FO 4490, and AN 923 (available from SNF Japan Co., Ltd.) can be used.
It is preferable that the ratio of the component 1 in the water-purifying agent that also contains the other additives such as the polymeric flocculant be 0.5% by mass or greater relative to the total amount of the water-purifying agent.
It is preferable that the ratio of the component 2 be 0.05% by mass or greater relative to the total amount of the water-purifying agent.
A wastewater treatment method of the present invention is for removing the inorganic unnecessary substance in wastewater by feeding the water-purifying agent of the present invention described above to the wastewater.
Examples of the inorganic unnecessary substance include an inorganic unnecessary substance that contains at least any one selected from the group consisting of nickel, fluorine, iron, copper, zinc, chromium, arsenic, cadmium, and lead.
The wastewater treatment method of the present invention will be specifically described.
For example, it is possible to add the water-purifying agent of the present invention after an insolubilizing step of adding a base to wastewater to make the wastewater basic, insolubilize at least part of the heavy metal ions, and form a suspended solid matter.
The water-purifying agent is fed to the wastewater to make the inorganic unnecessary substance flocculate and settle, and the settled, separated precipitate is removed. In this way, the wastewater is purified.
The present invention will be described below by way of Examples. The present invention should not be construed as being limited to these Examples.
As the plant, “intermediate jute No. 4”, which was Corchorus olitorius having an identification number 2013 in Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, was used.
A dried product (containing the component 1 in an amount of 0.56% by mass) of a plant including all leaves, stalks, and roots of the intermediate jute No. 4 (with a ratio of the leaves in the plant of 8% by mass) was used.
The intermediate jute No. 4 was dried and ground, and then separated through a sieve, in order to use a product with a size of 250 micrometers or less.
The component 1 being contained in an amount of 0.56% by mass in the dried product of the intermediate jute No. 4 used in the present Example was confirmed by performing the extracting operation described below.
That is, ethyl acetate was added to the dried product of the intermediate jute No. 4, to obtain a 10% by mass solution, which was left to stand still at room temperature (23° C.) for 8 hours, followed by filtration through a filter paper. The residue was washed with ethyl acetate, and then further subjected to extraction using distilled water, to obtain a supernatant, which was subjected to dialysis to separate a component having a fractionation molecular weight of 12,000 or greater, to obtain the component 1. Then, the ratio of the component 1 relative to the dried product of the intermediate jute No. 4, which was the raw material, was calculated.
To wastewater containing nickel, FeCl3 was added by 250 ppm as a primary flocculant, and then the water-purifying agent containing the dried product of Corchorus olitorius containing the component 1 in an amount of 0.56% by mass was added.
The initial Ni ion concentration was 60 ppm.
The result of the nickel ion concentration when the water-purifying agent of the present invention was added is presented in Table 3 below. As presented in Table 3, reduction in the nickel ion concentration was confirmed. With a nickel ion concentration of 8 ppm or lower, it can be judged that the water-purifying agent is non-problematic in practical use.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, the dried product of the intermediate jute No. 4 was changed to a dried product with a ratio of leaves of 10% by mass (containing the component 1 in an amount of 0.7% by mass). As presented in Table 3 below, reduction in the nickel ion concentration was confirmed in Example 2.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, the dried product of the intermediate jute No. 4 was changed to a dried product with a ratio of leaves of 100% by mass (containing the component 1 in an amount of 7.0% by mass). As presented in Table 3 below, reduction in the nickel ion concentration was confirmed in Example 3.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, the dried product of the intermediate jute No. 4 was changed to a dried product mainly containing stalks and roots but free of leaves (containing the component 1 in an amount of 0.1% by mass). As presented in Table 3 below, reduction in the nickel ion concentration was poor in Comparative Example 1.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, the dried product of the intermediate jute No. 4 was changed to a dried product with a ratio of leaves of 3% by mass (containing the component 1 in an amount of 0.21% by mass). As presented in Table 3 below, a sufficient reduction in the nickel ion concentration was not achieved in Comparative Example 2.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, the dried product of the intermediate jute No. 4 was changed to a dried product with a ratio of leaves of 5% by mass (containing the component 1 in an amount of 0.35% by mass). As presented in Table 3 below, a sufficient reduction in the nickel ion concentration was not achieved in Comparative Example 3.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, an extract formed of the component 1, extracted from the dried product of the intermediate jute No. 4, was used instead of the dried product of the intermediate jute No. 4, and this extract was directly added in an amount of 50 ppm. As presented in Table 3 below, reduction in the nickel ion concentration was confirmed in Example 4.
An experiment was performed in the same manner as in Example 4, except that unlike in Example 4, the extract formed of the component 1, extracted from the dried product of the intermediate jute No. 4, was added with the amount of addition changed to 5 ppm. As presented in Table 3 below, an excellent reduction in the nickel ion concentration that is the same as in Example 4 was confirmed with even such a small amount of addition.
An experiment was performed in the same manner as in Example 1, except that unlike in Example 1, as the kind of the plant, “intermediate jute No. 3”, which was Corchorus olitorius having an identification number in Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, i.e., varieties identification of registration No. 1209006 in Anhui province, was used instead of the intermediate jute No. 4.
Although the nickel ion concentration reducing effect was better in Example 1, a good nickel ion concentration reducing effect, which was almost the same as in Example 1, was exhibited in Example 6.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2016-181619 | Sep 2016 | JP | national |
| 2017-174021 | Sep 2017 | JP | national |
This application is a continuation of U.S. Application No. 16/333,440, filed Mar. 14, 2019, which is a U.S. National Stage Application of PCT/JP2017/032941, filed Sep. 12, 2017, which claims priority to Japanese Patent Application No. 2017-174021, filed Sep. 11, 2017, and Japanese Patent Application No. 2016-181619, filed Sep. 16, 2016, the entire contents of all of which are hereby incorporated by reference.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16333440 | Mar 2019 | US |
| Child | 18177182 | US |
