Claims
- 1. A method of extracting a bacterial antigen from a test sample which comprises the steps of:
- (a) reacting in a vessel a precursor reagent with a solid polymeric acid, or with a solid polycarboxylic acid anhydride capable of forming a polymeric acid, to form an extractive mixture, said pecursor reagent being capable of forming nitrous acid when reacted with said polymeric acid; and
- (b) incubating said sample with said extractive mixture to extract said antigen.
- 2. The method of claim 1 wherein said bacterial antigen comprises an antigenic carbohydrate.
- 3. The method of claim 1 wherein said bacterial antigen is a streptococcal antigen.
- 4. The method of claim 1 wherein said precursor reagent comprises a nitrite salt.
- 5. The method of claim 4 wherein said salt is sodium nitrite.
- 6. The method of claim 1 wherein said solid polymeric acid is a polycarboxylic acid.
- 7. The method of claim 1 wherein said solid polymeric acid is polysulfonic acid.
- 8. The method of claim 7 wherein said polysulfonic acid is polystyrene sulfonic acid.
- 9. The method of claim 1 wherein said solid polymeric acid is a polyphosphoric acid.
- 10. The method of claim 1 wherein said solid polymeric acid is a polyacrylic acid.
- 11. The method of claim 1 wherein said extractive mixture is formed by an acid consisting essentially of a polymethacrylic acid.
- 12. The method of claim 1 in which said polymeric acid or said polycarboxylic acid anhydride is provided in a test container, and said precursor reagent is added to said test container to form said extractive mixture.
- 13. A test kit comprising:
- a first vial including a solid polymeric acid or a solid polycarboxylic acid anhydride capable of forming a polymeric acid and
- a second vial including an extractive precursor reagent, said extractive precursor reagent being capable of forming nitrous acid when reacted with said polymeric acid.
- 14. A test kit as defined in claim 13 wherein said solid polymeric acid is a coating on an interior wall of said first vial.
- 15. A test kit as defined in claim 14 wherein said solid polymeric acid comprises a fluorescent compound.
- 16. A test kit as defined in claim 13 wherein said extractive precursor reagent comprises a nitrite salt.
- 17. A test kit as defined in claim 16 wherein said nitrite salt is sodium nitrite.
- 18. A test kit as defined in claim 13 wherein said solid polymeric acid is a polycarboxylic acid.
- 19. A test kit as defined in claim 13 wherein said solid polymeric acid is polysulfonic acid.
- 20. A test kit as defined in claim 19 wherein said polysulfonic acid is polystyrene sulfonic acid.
- 21. A test kit as defined in claim 13 wherein said solid polymeric acid is a polyphosphoric acid.
- 22. A test kit as defined in claim 13 wherein said solid polymeric acid is a polyacrylic acid.
- 23. A test kit as defined in claim 13 wherein said solid polymeric acid consists essentially of a polymethacrylic acid.
CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of my earlier, co-pending application U.S. Ser. No. 817,202 now abandoned, filed Jan. 8, 1986, which is hereby incorporated by reference.
US Referenced Citations (5)
Foreign Referenced Citations (1)
Number |
Date |
Country |
153477 |
Sep 1985 |
EPX |
Non-Patent Literature Citations (2)
Entry |
Kholy et al., (1974), Applied Microbiology 28(5):836-839. |
Hafez et al., (1981), J. Clin. Microbiol. 14(5):530-533. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
817202 |
Jan 1986 |
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