Claims
- 1. An isolated nucleic acid molecule encoding a MAP2 protein kinase designated ERK1, wherein the nucleotide sequence of said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleotide sequence comprising the coding region of ERK1 as set forth in SEQ ID No. 1;
- (b) a nucleotide sequence that hybridizes at high stringency to the nucleotide sequence of (a) and which encodes a polypeptide having the functional activity of ERK1; and
- (c) a nucleotide sequence differing from the sequences of (a) and (b) and which encodes a polypeptide encoded by the sequence of (a) or (b).
- 2. A vector which comprises the isolated nucleic acid molecule of claim 1.
- 3. The vector of claim 2, designated pBS-rERK1, deposited with the ATCC and having accession number 40808.
- 4. An expression vector comprising the nucleic acid molecule of claim 1, wherein the molecule is operatively linked to an expression control sequence.
- 5. A host-vector system for the production of a polypeptide having the functional activity of ERK1 which comprises the vector of claim 4, in a suitable host cell.
- 6. The host-vector system of claim 5, wherein the suitable host cell is a bacterial cell.
- 7. The host-vector system of claim 5, wherein the suitable host cell is a yeast cell.
- 8. The host-vector system of claim 5, wherein the suitable host cell is an insect cell.
- 9. The host-vector system of claim 5, wherein the suitable host cell is a mammalian cell.
- 10. An isolated nucleic acid molecule encoding a MAP2 protein kinase, ERK-1, having an amino acid sequence as depicted in SEQ ID No. 2.
- 11. The host-vector system of claim 6, wherein the bacterial cell is E. coli.
Parent Case Info
This application is a divisional of U.S. Ser. No. 08/178,488, filed Jan. 7, 1994, now abandoned which is a continuation of U.S. Ser. No. 07/701,544, filed May 16, 1991, now abandoned, which was a continuation-in-part of U.S. Ser. No. 07/532,004, filed Jun. 1, 1990, now abandoned, each of which is incorporated by reference herein in its entirety.
Government Interests
Pursuant to the provisions of 35 U.S.C. 202 (c), it is hereby acknowledged that the Government has certain rights in this invention, which was made in part with funds from the National Institutes of Health.
Non-Patent Literature Citations (5)
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Ray, L. B. et al. J. Biol. Chem. 263: 12721-12727, Sep. 1988. |
Kornbluth, S. et al. Molecular and Cellular Biology 8(12): 5541-5544, Dec. 1988. |
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Divisions (1)
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178488 |
Jan 1994 |
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Continuations (1)
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701544 |
May 1991 |
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Continuation in Parts (1)
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532004 |
Jun 1990 |
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