Claims
- 1. An isolated nucleic acid molecule encoding a MAP2 protein kinase known as ERK2, wherein the sequence of said nucleic acid is selected from the group consisting of:
- (a) a nucleic acid sequence comprising the coding region of ERK2 as set forth in FIG. 3A (SEQ ID NO: 3);
- (b) a nucleic acid sequence comprising a sequence that hybridizes at high stringency to SEQ ID NO: 3 and which encodes a polypeptide having the functional activity of ERK2; and
- (c) a nucleic acid sequence differing from the sequences of (a) and (b), which encodes a polypeptide encoded by the sequence of (a) or (b).
- 2. A vector which comprises the isolated nucleic acid molecule of claim 1.
- 3. The vector of claim 2, designated pBS-rERK2, deposited with the ATCC and having accession number 40809.
- 4. An expression vector comprising the nucleic acid molecule of claim 1, wherein the molecule is operatively linked to an expression control sequence.
- 5. A host-vector system for the production of a polypeptide having the functional activity of ERK2 which comprises the vector of claim 4, in a cultured host cell.
- 6. The host-vector system of claim 5, wherein the host cell is a bacterial cell.
- 7. The host-vector system of claim 5, wherein the host cell is a yeast cell.
- 8. The host-vector system of claim 5, wherein the host cell is an insect cell.
- 9. The host-vector system of claim 5, wherein the host cell is a mammalian cell.
- 10. An isolated nucleic acid molecule encoding a MAP2 protein kinase having an amino acid sequence as depicted in FIG. 3A (SEQ ID No. 4).
- 11. An isolated nucleic acid molecule encoding a polypeptide having the functional activity of ERK2, said polypeptide having an amino acid sequence which is a fragment of the amino acid sequence as depicted in FIG. 3A (SEQ ID No. 4).
- 12. A vector which comprises the isolated nucleic acid molecule of claim 11.
- 13. An expression vector comprising a nucleic acid molecule of claim 11, wherein the molecule is operatively linked to an expression control sequence.
- 14. A host-vector system for the production of a polypeptide having the functional activity of ERK2 which comprises the vector of claim 13, in a cultured host cell.
- 15. The host-vector system of claim 14, wherein the host cell is a bacterial cell.
- 16. The host-vector system of claim 14, wherein the host cell is a yeast cell.
- 17. The host-vector system of claim 14, wherein the host cell is an insect cell.
- 18. The host-vector system of claim 14, wherein the host cell is a mammalian cell.
- 19. The host-vector system of claim 6, wherein the bacterial cell is E. coli.
- 20. The host-vector system of claim 15, wherein the bacterial cell is E. coli.
Parent Case Info
This application is a divisional of U.S. Ser. No. 07/701,544, filed May 16, 1991, now abandoned, which was a continuation-in-part of U.S. Ser. No. 07/532,004, filed Jun. 1, 1990, now abandoned, each of which are incorporated by reference herein in their entireties.
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Divisions (1)
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Number |
Date |
Country |
Parent |
701544 |
May 1991 |
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Continuation in Parts (1)
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Number |
Date |
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532004 |
Jun 1990 |
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