Claims
- 1. A method of making a test kit for the determination of the presence of a substance having peroxidase-like activity comprising the steps of:
- providing a sheet having a specimen test area and a positive monitor area, said sheet comprising an inert water insoluble matrix;
- applying a test ink to said specimen test area and said positive monitor area, said test ink comprising an oxygen donor reagent and a chromogen reagent, said chromogen reagent oxidized by the oxygen donor reagent in the presence of a substance having the peroxidase-like activity, to provide a visually observable change of color, said oxygen donor reagent and said chromogen reagent being mixed as a single solution with a surfactant before being applied to said sheet;
- said single solution maintaining the oxygen donor reagent and the chromogen reagent inert relative to each other in the absence of an aqueous solution; and
- applying a positive monitor ink on said test ink in said positive monitor area, said positive monitor ink comprising a substance having the peroxidase-like activity.
- 2. The method of claim 1 wherein said sheet further comprises a negative control area characterized by the absence of test ink and positive monitor ink.
- 3. The method of claim 1 wherein the applying of said inks involves the step of printing.
- 4. The method of claim 1 wherein the chromogen is 3,3',5,5'-tetramethylbenzidine and the chromogen and oxygen donor reagents are encapsulated with a water soluble polymer for providing a moisture barrier prior to the step of applying the test ink to the sheet.
- 5. The method of claim 1 wherein said oxygen donor reagent is cumene hydroperoxide.
- 6. The method of claim 1 wherein said surfactant is added to the chromogen reagent before mixing with the oxygen donor reagent.
- 7. The method of claim 6 wherein said surfactant is also added to the oxygen donor reagent before mixing with the chromogen reagent and added surfactant.
- 8. The method of claim 6 wherein said surfactant is alkylphenoxypolyethoxyethanol.
- 9. The method of claim 7 wherein said surfactant is alkylphenoxypolyethoxyethanol.
- 10. The method of claim 1 wherein said surfactant is added to the oxygen donor reagent before mixing with the chromogen reagent.
- 11. The method of claim 10 wherein said surfactant is alkylphenoxypolyethoxyethanol.
- 12. The method of claim 1 wherein said chromogen reagent is 3,3',5,5'-tetramethylbenzidine.
- 13. The method of claim 1 including the additional step of applying a water soluble polymer over the entire sheet.
- 14. The method of claim 13 wherein the water soluble polymer is polyvinylpyrrolidone.
Parent Case Info
This is a division of application Ser. No. 07/333,828 filed Apr. 4, 1989, which is pending.
US Referenced Citations (117)
Foreign Referenced Citations (7)
Number |
Date |
Country |
0093595 |
Apr 1983 |
EPX |
093595 |
Nov 1983 |
EPX |
0124215 |
Feb 1984 |
EPX |
0222700 |
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Divisions (1)
|
Number |
Date |
Country |
Parent |
333828 |
Apr 1989 |
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