Patent Application
20110269835
The present invention relates to a method for inhibiting (antagonizing) male Synanthedon scitula attraction to female Synanthedon scitula, comprising exposing a Synanthedon scitula population to a composition comprising E,Z-3,13-octadecadienyl acetate in a quantity sufficient to inhibit (antagonize) male Synanthedon scitula attraction to female Synanthedon scitula, and optionally a carrier material or carrier.
In addition, the present invention relates to a composition for inhibiting (antagonizing) male Synanthedon scitula attraction to female Synanthedon scitula, containing E,Z-3,13-octadecadienyl acetate in a quantity sufficient to inhibit male Synanthedon scitula attraction to female Synanthedon scitula, and optionally a carrier material or carrier.
The dogwood borer (DWB), Synanthedon scitula (Harris) (Lepidoptera: Sesiidae), not only is an important pest of dogwood but also causes severe problems on at least 19 species of fruit, nut, and ornamental trees in the eastern United States and Canada (Bergh, J. C., and T. C. Leskey, Cana. Entomol., 135: 615-635 (2003); Eichlin, T. D., and W. D. Duckworth, Sesioidea: Sesiidae, pp. 1-176, In R. B. Dominick (ed.), The moths of America North of Mexico, fascicle 5.1, Wedge Entomological Research Foundation, Washington, D.C. (1988); Engelhardt, G. P., J. Econ. Entomol., 25: 239-294 (1932)). DWB more recently has become an increasingly economically important pest of apples (Kain, D. and R. W. Straub, N.Y. Fruit. Quart., 9: 10-12 (2001); Riedl, H., et al., Can. Entomol., 117: 1367-1377 (1985); Warner, J., and S. Hay, Can. Entomol., 117: 1471-1478 (1985)). The ultimate factor responsible for the increased abundance of DWB in apples is the use of clonal, size-controlling rootstocks in high-density apple orchards due to international competition in the apple market (Marshall, D. W., and P. K. Andrews, HortTechnology, 4: 1-16 (1994)). Commercially available pheromone lures for dogwood borer rely on generalized sex pheromone components identified from other Sesiid species (Nielsen, D. G., et al., EAG and field responses of sesiid males to sex pheromones and related compounds, pp. 11-26, In J. W. Neal (ed.), Pheromones of the Sesiidae, SEA, U.S. Dept. Agric., ARR-NE-6, Washington. DC (1979); Tumlinson, J. H., et al., Science (Wash DC), 185: 614-616 (1974)). Unfortunately, the commercially available pheromone lures have produced unreliable results (Bergh, J. C., et al., J. Econ. Entomol., 97: 344-352 (2003); Braxton, S. M., and M. J. Raupp, J. Arbor., 21: 177-180 (1995); Davidson, J. A., et al., J. Arbor., 18: 81-84 (1992); Meyer, W. L., and W. S. Cranshaw, Southwest Entomol., 19: 71-76 (1994); Pfeiffer, D. G., and J. C. Killian, J. Entomol. Sci., 34: 210-218 (1999); Riedl, H., et al., Cana. Entomol., 117: 1367-1377 (1985); Snow, J. W., et al., J. Agric. Entomol., 2: 73-84 (1985); Bergh et al., J. Econ. Entomol. 97: 344-352 (2004)).
Bergh and Leskey (Canadian Entomol., 135: 615-635 (2003)) on page 630 alleged the following regarding research conducted by Pfeiffer et al. (J. Entomol. Sci., 34: 210-218 (1999)): “ . . . In the only published dogwood borer mating disruption trial, Pfeiffer and Killian (1999) deployed Isomate-P (peachtree borer) . . . pheromone dispensers (96:4 blend of (Z,Z)-3,13-ODDA to (E,Z)-3,13-ODDA) in 2-ha pots of ‘Gala’ apples in Virginia. Capture of male dogwood borer in pheromone traps was reduced by nearly 100% in 3 consecutive years . . . . ” However, the trap capture data used to monitor the efficacy of the trial was not accurate because Pfeiffer's traps were baited with lures formulated with a commercial generic Sesiidae pheromone blend, and not the actual three-component dogwood borer pheromone containing Z,Z-3,13-octadecadienyl acetate, E,Z-2,13-octadecadienyl acetate, and Z,E-3,13-octadecadienyl acetate (as first disclosed in U.S. Provisional Application No. 60/662,067, filed 14 Mar. 2005; U.S. patent application Ser. No. 11/372,173, filed 9 Mar. 2006; and U.S. patent application Ser. No. 12/327,010, filed 3 Dec. 2008). Numerous studies in the literature (e.g., reviewed in Bergh and Leskey 2003) have reported inconsistent results and poor trap captures of dogwood borer using commercially available pheromone lures for that pest. For example, Davidson, J. A., et al., “Controlling clearwing moths with entomopathogenic nematodes: The dogwood borer case study”, Journal of Arboriculture, 18(2): 81-84 (1992), states on page 83: “ . . . Unfortunately, from our experience over several years, commercially available dogwood borer pheromone traps cannot be relied on in Maryland for monitoring periods of adult moth flight activity . . . . ” The only way to effectively discern if a mating inhibition or disruption formulation is efficacious is to deploy a lure based on the female moth's true pheromone blend containing Z,Z-3,13-octadecadienyl acetate, E,Z-2,13-octadecadienyl acetate, and Z,E-3,13-octadecadienyl acetate. At the time of these past trials, the true S. scitula pheromone was not identified and therefore pheromone lures for monitoring mating inhibition and disruption trials were unavailable.
Thus a method and composition for inhibiting (antagonizing) male Synanthedon scitula attraction to female Synanthedon scitula is needed.
In accordance with the present invention there is provided a method for inhibiting (antagonizing) male Synanthedon scitula attraction to female Synanthedon scitula, comprising exposing a Synanthedon scitula population to a composition comprising E,Z-3,13-octadecadienyl acetate in a quantity sufficient to inhibit (antagonize) male Synanthedon scitula attraction to female Synanthedon scitula, and optionally a carrier material or carrier.
Also in accordance with the present invention is a composition for inhibiting (antagonizing) male Synanthedon scitula attraction to female Synanthedon scitula, containing E,Z-3,13-octadecadienyl acetate in a quantity sufficient to inhibit (antagonize) male Synanthedon scitula attraction to female Synanthedon scitula, and optionally a carrier material or carrier.
This summary is provided to introduce a selection of concepts in a simplified form that are further described below in the detailed description. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended as an aid in determining the scope of the claimed subject matter.
The compound described herein (i.e., E,Z-3,13-octadecadienyl acetate) may be applied with a carrier component or carrier (e.g., agronomically or physiologically or pharmaceutically acceptable carrier). The carrier component can be a liquid or a solid material. As is known in the art, the vehicle or carrier to be used refers to a substrate such as a membrane, hollow fiber, microcapsule, cigarette filter, gel, polymers, septa, or the like. All of these substrates have been used to release insect pheromones in general and are well known in the art. Suitable carriers are well-known in the art and are selected in accordance with the ultimate application of interest. Agronomically acceptable substances include aqueous solutions, glycols, alcohols, ketones, esters, hydrocarbons halogenated hydrocarbons, polyvinyl chloride; in addition, solid carriers such as clays, cellulosic and rubber materials and synthetic polymers. The carrier or carrier material as used herein is defined as not including the body of an insect (e.g., Synanthedon scitula).
In the case where the desired response is inhibiting (antagonizing) male DWB attraction to female DWB or female sex pheromone through the use of E,Z-3,13-octadecadienyl acetate, an “effective amount” is defined as that quantity of E,Z-3,13-octadecadienyl acetate which prevents male DWB from orienting to the female DWB (and inseminating the female DWB); in other words, an effective amount is a quantity sufficient to prevent potential mates from finding each other, thus inhibiting (antagonizing) male DWB attraction to female dogwood borers or reducing the probability that the dogwood borers will mate. Factors such as population density, temperature, wind velocity and release rate will influence the actual number of DWB inhibited (antagonized). The precise amount of the composition can easily be determined by one skilled in the art given the teaching of this application. For example, the exact dose to use in any particular set of circumstances can readily be determined by a dose response field test or one skilled in the art could follow the procedures utilized below; the composition would inhibit (antagonize) male DWB attraction to female DWB or female sex pheromone by more than 50% and would be statistically significant in comparison to a control. Preferably the composition contains at least 0.5% by weight E,Z-3,13-octadecadienyl acetate. Effective loadings of the E,Z-3,13-ODDA in the composition may vary between about 1 to about 100 (e.g., 1-100) mg/dispenser (preferably about 1-about 50 (e.g., 1-50) mg/dispenser, more preferably about 10-about 30 (e.g., 10-30) mg/dispenser). The composition may or may not contain a control agent for Synanthedon scitula, such as a biological control agent or an insecticide known in the art to kill Synanthedon scitula. Other compounds may be added to the composition provided they do not substantially interfere with the intended activity of the composition; whether or not a compound interferes with inhibiting (antagonizing) male DWB attraction to female DWB or female sex pheromone can be determined, for example, by the procedures utilized below.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. The term “about” is defined as plus or minus ten percent; for example, about 100° F. means 90° F. to 110° F. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described.
The following examples are intended only to further illustrate the invention and are not intended to limit the scope of the invention as defined by the claims.
Insects: Larvae of DWB were excavated from burr knot tissue of apple trees (Jefferson and Berkeley Counties, W. Va, and Frederick County, Va.) with obvious signs of infestation including frass and entry wounds in October and November. Larvae were brought back to the laboratory and reared on general purpose Lepidopteran diet (Bioserv, Frenchtown N.J.) in an incubator at 25° C. (16L:8D) until pupation. Pupae were sexed according to the characteristics described by Leskey and Bergh (Leskey, T. C., and J. C. Bergh, Florida Entomol., 86: 379-381 (2003)) and held in 1 oz. clear plastic cups (Jet Plastica Industries, Hatfield Pa.) with a small piece of moistened cotton dental wick and topped with plastic caps. Pupae were then shipped by express carrier or delivered to Beltsville, Md. in January. Upon arrival, the pupae were kept in a in an insectary at 25° C. and 16L:8D photoperiod until adult emergence. Absorbent cotton moistened with 8% sugar water was provided as food source for emerged moths. Moths were sexed and females were transferred to effluvial collection device for volatiles collection.
Effluvial Collections: Volatiles were collected using six groups of 1- to 13-day-old virgin females (3 to 10 females per group) at room temperature and 16L:8D photoperiod. The moths were separately introduced into three 1-1, 4-necked glass containers (Zhang, A., et al., J. Chem. Ecol., 20: 2415-2427 (1994)). Air was drawn into the container through 6-14 mesh activated charcoal (Fisher Scientific, Pittsburgh, Pa.), and out of the container through two traps (15 cm×1.5-cm o.d.) containing Super Q (200 mg each; Alltech Associates, Inc., Deerfield, Ill.) by vacuum (˜1 l/min). Female moths were fed with 10% sugar solution on cotton balls and aerated continuously for 3-7 days at room temperature and 16L:8D photoperiod. The adsorbent traps were changed every 48 hr. Adsorbents were eluted with methylene chloride (4×0.5 ml); the eluates (2 ml/each sample) were concentrated to ˜20 μl under a nitrogen stream and stored at −30° C. for future analysis.
Pheromone Gland Extractions: Pheromone gland extracts were obtained during photophase from six groups of 2- to 13-day-old virgin females that had been used in aeration. A female abdomen was compressed gently until the ovipositor everted from the abdominal tip. The ovipositor was then excised with micro scissors into a conical glass vial containing ˜100 μl methylene chloride-methanol (3:1). The glands were soaked for at least 2 hr at room temperature. The extracts were then removed, and the glands were re-extracted with 100 μl methylene chloride-methanol. The combined solution was concentrated to ˜20 μl under nitrogen stream and kept at −30° C. in a freezer for future analysis.
Electrophysiological Recordings and Mass Spectrometry: The coupled gas chromatographic-electroantennographic detection (GC-EAD) system used was as previously described (Zhang, A., and S. Polavarapu, J. Chem. Ecol., 29: 2153-2164 (2003); Zhang, A., and S. Polavarapu, J. Chem. Ecol., 30: 1531-1545 (2004); Zhang, A., et al., J. Chem. Ecol., 23: 231-245 (1997)). A Hewlett Packard (HP) 6890 gas chromatograph equipped with a 60 m×0.25-mm i.d., 0.25-mm film-thickness DB-WAXETR capillary column (J&W Scientific Inc., Folsom, Calif., 120° C. for 2 min, then programmed to 250° C. at 10° C./min and held for 10 min) or a 60 m×0.25-mm i.d., 0.25-mm film-thickness DB-5 capillary column (J&W Scientific Inc., 100° C. for 2 min, then programmed to 250° C. at 15° C./min and held for 10 min) in the splitless mode with hydrogen as carrier (1.4 ml/min) was used for GC-EAD analysis. Electronic impact (EI) gas chromatography-mass spectrometry (GC-MS) was conducted on a Hewlett-Packard 6890 GC coupled to a HP 5973 Mass Selective Detector using an identical DB-WAXETR capillary column (120° C. for 2 min, then programmed to 230° C. at 15° C./min and held for 25 min for regular analysis, but with helium as carrier gas. A 70 eV electron beam was employed for sample ionization.
Chemicals: (Z,Z)-3,13-octadecadienyl acetate was purchased from Bedoukian Research, Danbury Conn. (˜90% purity). It was purified by flush chromatography using 2:10 of CH2Cl2:hexane as the mobile phase on 15% AgNO3 in silica gel 60 (EM Science, 230-400 mesh). The fractions with the impurity of E,Z-3,13-ODDA less than 0.3% based on the molar amount of the Z,Z-3,13-octadecadienyl acetate were combined and solvents were evaporated using a Büchi RE 111 rotary evaporator. All other synthetic pheromone standards were purchased from Pheromone Bank, Wageningen, The Netherlands (>96% purity). Purities of chemicals were checked on a 60 m polar DB-WAXETR GC capillary column before preparing the lures for the field study.
Lure Analysis: The binary and trinary lures exposed in the field during 6 wk, 8 wk, and 12 wk were collected and placed individually into 20 ml hexane in a 25 ml vial and soaked for 48 hr. GC analyses of pheromone lures were conducted on a HP 6890 GC coupled to a DB-5 capillary column by injecting of 1 ml of each extract. The pure Z,Z-3,13-ODDA were used as the standard and remaining pheromone concentrations were obtained by comparison with this standard at the same conditions.
Field Trapping Tests: Red natural rubber septa (5 mm, Wheaton, N.J.) loaded with the desired rates of Z,Z-3,13-ODDA and the blends in ˜20 ml of hexane solution were used for field deployment. The same amount of solvent (hexane) was loaded on the septum for the blank control. After loading, the solvent was allowed to evaporate in a fume hood for 30 min. Lures were then wrapped in aluminum foil, stored in 20 ml plastic vials, and shipped by express carrier on the same day. Upon arrival, the lures were kept in a freezer at −10° C. until deployed. All field tests were conducted in commercial apple fields in WV, VA, and NC using Pherocon 1C or Delta sticky traps (Trécé, Salinas, Calif.). Traps were placed in trees at ˜1.2 m above the ground (Riedl, H., et al., Cana. Entomol., 117: 1367-1377 (1985)). At each location, experimental lures were randomized within each of three to five rows (depending on the number of replicates per test) and separated by at least one buffer row, and traps were spaced at a minimum of ˜25 m intervals within a row. Traps were rotated among positions within each row at weekly intervals for the duration of each test. The number of dogwood borer, peachtree borer, lilac borer, and other male Sesiidae moths captured were recorded weekly.
In the first comparison trial, the following lures were deployed: the single component (100% Z,Z-3,13 ODDA); the binary blend (Z,Z-3,13-ODDA : E,Z-2,13-ODDA=94:6); a second two-component blend (Z,Z-3,13-ODDA : Z,E-3,13-ODDA=94:6, based on the molar amount of the Z,Z-3,13-octadecadienyl acetate in the composition); the trinary blend (Z,Z-3,13-ODDA:E,Z-2,13-ODDA:Z,E-3,13-ODDA=88:6:6); and control. Next, to evaluate the attractive effect of addition of third component, 0.5, 1, 3, or 10% based on the molar amount of the Z,Z-3,13-octadecadienyl acetate of Z,E-3,13-ODDA were added into the binary blend individually. Similarly, 0.5, 1, 3, or 10% of E,Z-2,13-ODDA were individually added into a blend consisted of Z,Z-3,13-ODDA:Z,E-3,13-ODDA (94:6) to evaluate the effect of E,Z-2,13-ODDA. In another comparison, 0.5, 1, 2, or 3% E,Z-3,13-ODDA was added to the trinary blend. Dose response tests of the trinary and binary blends were deployed in each location, with doses consisting of 10-μg, 100-μg, 300-μg, 1-mg, and 10-mg (trinary blend); and 10-μg, 100-μg, 300-μg, 500-μg, and 1-mg (binary blend), respectively. Caged live virgin females (2-4 days old and 1 female per cage) were deployed in traps to provide a natural source of pheromone for comparison with traps baited with the binary and trinary blends. In a final comparison to determine over what distance DWB can detect the trinary blend, we compared captures in traps baited with the trinary blend deployed within an infested apple orchard, and 50 and 100 m outside the orchard within a large wood lot habitat.
Statistics: The data on control traps were omitted in the analysis because of zero trap catches. Unless otherwise specified, data on trap catches from each test were logarithm transformed (logχ+1) to normalize the variance before analysis. Means were compared by one-way analysis of variance (ANOVA) followed by Ryan-Einot-Gabriel-Welsch Range test (SPSS 10.0 for Windows, George and Mallery, 2002) for significance at α=0.05.
Results. Analysis of Sex Pheromone Components in Female Effluvial Collections and Gland Extracts: A typical coupled GC-EAD graph is showed in
Field Trapping Tests: Male trap captures from initial field studies using septa containing 1-mg of the single-component, the binary blend, a two-component blend, and the trinary blend are summarized in
Addition of 0.5 to 10% of Z,E-3,13-ODDA to the binary blend did not cause a significant increase in attraction to the binary blend (
In the consequent test we found that a geometric isomer, E,Z-3,13-ODDA, surprisingly reduced trap captures of DWB significantly. Surprisingly, captures in traps baited with our most attractive lure (trinary blend) were surprisingly significantly inhibited (antagonized) by addition of as little as 0.5-1% E,Z-3,13-ODDA (
Data from dose response tests with the trinary blend demonstrated that male DWB catches were significantly lower in traps baited with 10-:g compared to captures in traps baited with any other doses tested. When dose was increased to 1-mg and above, trap captures were significantly higher compared to 100-300-:g doses (
Male DWB were attracted to traps baited with the trinary blend at distances of at least 50 m away from a highly infested orchard (
In the final field test, we evaluated the efficacy of the binary and trinary blends compared to a virgin female. Surprisingly, rubber septa loaded with 1-mg dose of the binary and trinary blends attracted significantly more males than traps baited with one virgin female (
Species-specificity of experimental blends was surprisingly high. Male dogwood borers represented from 97.1-99.2% of captures with the trinary blend and from 97.8-99.2% of captures with the binary blend across all sites (
Lure longevity and release rate were evaluated using lures loaded with the binary and trinary blends and exposed in the field for 6, 8, and 12 wk. GC analyses indicated that ˜13 percent of the pheromone was evaporated and ˜0.87-mg pheromone remained in the rubber septa lures exposed for 6 wk in the field from May to June in North Carolina, ˜18 percent of the pheromone was evaporated and ˜0.82-mg pheromone remained in the rubber septa lures exposed for 8 wk in the field from May to July in West Virginia, and ˜24 percent of the pheromone was evaporated and ˜0.76-mg pheromone still remained in the rubber septa lures exposed for 12 wk in the field from June to August in Virginia (Table 2). During these field tests, more than 26,000 males were caught in those binary and trinary blends-baited traps, whereas control traps caught no males.
On the basis of GC-EAD analyses of effluvial collections, gland extracts, synthetic isomers of 3,13- and 2,13-ODDA, and field trapping tests, we have demonstrated that Z,Z-3,13-ODDA was the main pheromone component of female DWB and it alone will reliably attract male DWB. However, we have surprisingly found that other components significantly increased attraction. When Z,Z,-3,13-ODD was combined with E,Z-2,13-ODDA in a 94:6 blend, captures were significantly greater than Z,Z-3,13-ODDA alone or Z,Z-3,13-ODDA in combination with Z,E-3,13-ODDA in 94:6 blend. Ultimately, though, the significantly greatest captures were recorded for traps baited with the trinary blend (Z,Z-3,13-ODDA : E,Z-2,13-ODDA:Z,E-3,13-ODDA=88:6:6), compared to any other treatment across all locations (
We found that two pheromone components, Z,Z-3,13-ODDA and E,Z-2,13-ODDA, were surprisingly absolutely essential for attraction of male DWB. When as little as 1% E,Z-2,13-ODDA was added to a 94:6 (Z,Z-3,13-ODDA : Z,E-3,13-ODDA) blend, captures surprisingly increased significantly in all locations (
However, without being bound by theory, we believe that Z,E-3,13-ODDA does synergize the response to the binary blend (Z,Z-3,13-ODDA:E,Z-2,13-ODDA) based on the fact that significantly more captures were recorded in all locations with this trinary blend compared to the binary blend (
We have documented the surprising pheromone antagonistic effect of E,Z-3,13-ODDA on Z,Z-3,13-ODDA. In field trials, surprising as little as 0.5% E,Z-3,13-ODDA added to our trinary blend significantly reduced trap captures in all locations (
In terms of dose responses to the binary and trinary blends, significantly more male DWB were captured in traps baited with septa loaded with greater than 10-μg of material (
Selectivity of pheromone lures is a critical factor in optimizing their effectiveness as monitoring tools. The percentage of dogwood borers captured with the trinary and binary blends was surprisingly greater than 97% across all locations, while the specificity of the Scenturion® lure for dogwood borers was much lower, ranging from 6.3-74.4% of total trap captures across locations (
The results from release rate and lure longevity studies indicated that pheromone lures with 1-mg loading should have an effective lifetime over the whole flight season. This experiment was terminated only because of the seasonal decline in DWB populations rather than decline in the lure attractiveness. Chemical analyses of lures revealed that ˜0.76-mg of pheromone remained in the 1-mg dose septa after 12 wk of field exposure (Table 2). These amounts of residual pheromones should still be sufficient to trigger and maintain the lure attraction for some time. Considering that release rate was affected by the temperature as well as wind speed, the dose of 1-mg lures did not need to be changed for the entire test season.
All of the references cited herein are incorporated by reference in their entirety. Also incorporated by reference in their entirety are the following references: Evenden, M. L., et al., J. Chem. Ecol., 25: 501-517 (1999); Evenden, M. L., et al., J. Econ. Entomol., 92:380-390 (1999); George, D., and P. Mallery, SPSS for Windows step by step: A simple guide and reference, 4th ed., 2002, Allyn & Bacon, Boston; Greenfield, M. D., Niche segregation of adult clearwing moths (Lepidoptera: Sesiidae) in Wisconsin, Ph.D. Thesis, University of Wisconsin, Madison, Wis., 138 pp. (1978); Karandinos, M. G., et al., J. Chem. Ecol., 3:57-64 (1977); Leskey, T. C., J. C. Bergh, J. F. Walgenbach, and A. Zhang, Improved attractiveness and specificity of pheromone-baited traps for male dogwood borer, Synanthedon scitula (Harris) (Lepidoptera: Sesiidae), Environ. Entomol. (submitted); McLaughlin, J. R., et al., Fl. Entomol., 60:27-29 (1977)); Nielsen, D. G., et al., Environ. Entomol., 4: 451-454 (1975); Pfeiffer, D. G., and J. C. Killian, J. Entomol. Sci., 34: 210-218 (1999); Tumlinson, J. H., 1979, The chemistry of Sesiidae pheromones, pp. 1-10, In Pheromones of the Sesiidae (formerly Aegeriidae), US Department of Agriculture, Science and Education Administration, AAR-NE-6, Beltsville, Md. Also incorporated by reference in their entirety are the following U.S. Pat. Nos. 6,703,014; 6,479,046.
Thus, in view of the above, the present invention concerns (in part) the following:
A (synthetic or isolated or purified) composition, comprising (or consisting essentially of or consisting of) E,Z-3,13-octadecadienyl acetate, and optionally a carrier material or carrier (e.g., an agronomically or physiologically or pharmaceutically acceptable carrier).
The above composition, said composition containing about 0% Z,Z-3,13-octadecadienyl acetate, about 0% E,Z-2,13-octadecadienyl acetate, and about 0% Z,E-3,13-octadecadienyl acetate. The above composition, said composition containing no Z,Z-3,13-octadecadienyl acetate. The above composition, said composition containing no E,Z-2,13-octadecadienyl acetate. The above composition, said composition containing no Z,E-3,13-octadecadienyl acetate. The above composition, said composition containing no Z,Z-3,13-octadecadienyl acetate, E,Z-2,13-octadecadienyl acetate, and Z,E-3,13-octadecadienyl acetate.
A method for inhibiting male Synanthedon scitula attraction to female Synanthedon scitula, comprising (or consisting essentially of or consisting of) exposing a Synanthedon scitula population to a composition comprising (or consisting essentially of or consisting of) E,Z-3,13-octadecadienyl acetate in a quantity sufficient to inhibit male Synanthedon scitula attraction to female Synanthedon scitula, and optionally a carrier material or carrier (e.g., an agronomically or physiologically (pharmaceutically) acceptable carrier). The above method, said composition does not contain Z,Z-3,13-octadecadienyl acetate. The above method, said composition does not contain E,Z-2,13-octadecadienyl acetate. The above method, said composition does not contain Z,E-3,13-octadecadienyl acetate. The above method, said composition does not contain Z,Z-3,13-octadecadienyl acetate, E,Z-2,13-octadecadienyl acetate, and Z,E-3,13-octadecadienyl acetate. The above method, said method does not utilize Z,Z-3,13-octadecadienyl acetate. The above method, said method does not utilize E,Z-2,13-octadecadienyl acetate. The above method, said method does not utilize Z,E-3,13-octadecadienyl acetate. The above method, said method does not utilize Z,Z-3,13-octadecadienyl acetate, E,Z-2,13-octadecadienyl acetate, and Z,E-3,13-octadecadienyl acetate.
The above method, said composition comprising (or consisting essentially of) at least 0.5% by weight E,Z-3,13-octadecadienyl acetate. The above method, said composition comprising (or consisting essentially of) at least 1% by weight E,Z-3,13-octadecadienyl acetate. The above method, said composition comprising (or consisting essentially of) at least 5% by weight E,Z-3,13-octadecadienyl acetate.
The carrier or carrier material as used herein is defined as not including the body of an insect (e.g., male and/or female Synanthedon scitula).
Other embodiments of the invention will be apparent to those skilled in the art from a consideration of this specification or practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope and spirit of the invention being indicated by the following claims.
This application claims the benefit of U.S. Provisional Application No. 60/662,067, filed 14 Mar. 2005, U.S. patent application Ser. No. 11/372,173, filed 9 Mar. 2006, and U.S. patent application Ser. No. 12/327,010, filed 3 Dec. 2008, which are incorporated herein by reference in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| 60662067 | Mar 2005 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 12327010 | Dec 2008 | US |
| Child | 13081932 | US | |
| Parent | 11372173 | Mar 2006 | US |
| Child | 12327010 | US |
