Claims
- 1. A method for producing migrastatin comprising the fermentation of Streptomyces platensis under conditions such that migrastatin is produced.
- 2. The method of claim 1, wherein said Streptomyces platensis is NRRL 18993.
- 3. A migrastatin analog obtainable from fermentation broth of Streptomyces platensis NRRL 18993 fermented under conditions such that migrastatin is produced.
- 4. The migrastatin analog of claim 3 that is isomigrastatin.
- 5. The method of claim 1, wherein said fermentation is conducted in a medium comprising a resin that binds migrastatin.
- 6. The method of claim 5, wherein said resin is XAD resin.
- 7. A method of purifying migrastatin or isomigrastatin from a fermentation medium, said method comprising steps of (1) solid phase extraction; (2) extraction with an organic solvent, and (3) chromatography.
- 8. The method of claim 7, wherein said steps are (1) solid phase extraction; (2) extraction with an methanol, (3) chromatography with C18 resin; and (4) extraction with acetone.
- 9. A pharmaceutical formulation comprising isomigrastatin.
- 10. A method to treat cancer in a human or animal, said method comprising administering a therapeutically effective dose of a formulation of claim 9.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is entitled to the benefit of the filing date of Aug. 21, 2000, for U.S. Provisional patent application Serial No. 60/226,595, entitled FERMENTATION AND PURIFICATION OF MIGRASTATIN, incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
|
60226595 |
Aug 2000 |
US |