Claims
- 1. A method for the production of mycolactones, which method comprises cultivation of Mycobacterium ulcerans in a dispersed suspension culture by fermentation or in spinner flasks.
- 2. The method of claim 1, wherein the mycolactones are produced in concentrations greater than that observed in T flasks.
- 3. The method of claim 1, wherein said cells are cultivated in M7H9 medium.
- 4. The method of claim 3, wherein said medium is enriched.
- 5. The method of claim 4, wherein said medium is enriched by addition of egg yolk.
- 6. A method for the purification of mycolactones that comprises extraction of media in which Mycobacterium ulcerans has been cultivated and chromatographic separation of said extract.
- 7. Mycolactone C in substantially purified form.
- 8. Mycolactone D in substantially purified form.
Parent Case Info
[0001] This application asserts priority to U.S. Provisional Application No. 60/222,649 filed Aug. 3, 2000 by inventors Peter Licari, Robert Arslanian, Lawrence Cadapan and John Carney entitled FERMENTATION AND PURIFICATION OF MYCOLACTONES which is incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
|
60222649 |
Aug 2000 |
US |