Claims
- 1. A method for producing a demethylmaytansinoid compound of the formula: ##STR23## (wherein X is Cl or H; R.sub.1 is H or acyl) characterized in that a maytansinoid compound of the formula: ##STR24## (wherein X and R.sub.1 are as respectively defined above) is contacted with a culture broth, inclusive of a processed matter of the culture broth, of a microorganism belonging to the genus Bacillus, the genus Streptomyces or the genus Actinomyces which is able to transform the 20-methoxy group of said maytansinoid compound into a hydroxy group.
- 2. A method as claimed in claim 1, wherein the microorganism is Bacillus megaterium, Streptomyces flavotricini, Streptomyces platensis, Streptomyces libani or Actinomyces nigrescens.
- 3. A method as claimed in claim 2, wherein the microorganism is Bacillus megaterium IFO 12108 Streptomyces flavotricini IFO 12770, Streptomyces platensis IFO 12901, Streptomyces libani IFO 13452 or Actinomyces nigrescens IFO 12894.
- 4. A method for producing a demethylmaytansinoid compound of the formula: ##STR25## (wherein X is Cl or H) characterized in that a maytansinoid compound of the formula: ##STR26## (wherein X is as defined above; R.sub.1 ' is acyl group) is contacted with a culture broth, inclusive of a processed matter of the culture broth, of a microorganism of the genus Bacillus, the genus Streptomyces or the genus Actinomyces which is able to transform the 20-methoxy group of said maytansinoid compound into a hydroxy group to obtain a compound of the formula: ##STR27## (wherein X and R.sub.1 ' are as respectively defined above) and this latter compound is then subjected to deacylation.
- 5. A method as claimed in claim 4, wherein the microorganism is Bacillus megaterium, Streptomyces flavotricini, Streptomyces platensis, Streptomyces libani or Actinomyces nigrescens.
- 6. A method as claimed in claim 5, wherein the microorganism is Bacillus megaterium IFO 12108, Streptomyces flavotricini IFO 12770, Streptomyces platensis IFO 12901, Streptomyces libani IFO 13452 or Actinomyces nigrescens IFO 12894.
- 7. A method as claimed in claim 4, wherein the deacylation is conducted by employing a complex metal hydride.
- 8. A method as claimed in claim 7, wherein the complex metal hydride is lithium aluminum hydride.
Priority Claims (4)
Number |
Date |
Country |
Kind |
53/34645 |
Mar 1978 |
JPX |
|
53/160787 |
Dec 1978 |
JPX |
|
54/119959 |
Sep 1979 |
JPX |
|
2968(1979)[U] |
Mar 1979 |
KRX |
|
Parent Case Info
This application is a divisional of application Ser. No. 153,522 filed May 27, 1980, now U.S. Pat. No. 4,307,016, which is a continuation-in-part of Ser. No. 19,612 filed Mar. 12, 1979, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4151042 |
Higashide et al. |
Apr 1979 |
|
4225494 |
Higashide et al. |
Sep 1980 |
|
4322348 |
Asai et al. |
Mar 1982 |
|
Divisions (1)
|
Number |
Date |
Country |
Parent |
153522 |
May 1980 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
19612 |
Mar 1979 |
|