Claims
- 1. Hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding ability wherein said sequence comprises at least one of the nucleotide sequences ##STR1##
- 2. A microorganism transformed by a hybrid-DNA-molecule of claim 1.
- 3. Plasmid or phage comprising one or more hybrid-DNA-molecules according to claim 1.
- 4. Microorganism containing at least a plasmid or phage according to claim 3.
- 5. A method for producing a fibronectin binding protein which comprises:
- a) transforming a suitable host cell with a hybrid-DNA-molecule of claim 1,
- b) harvesting the cells of (a) and culture medium of said cells and
- c) isolating the fibronectin binding protein from the cells and culture medium of (b) by a process which comprises fibronectin affinity chromatography followed by ion exchange chromatography.
- 6. Hybrid-DNA-molecule coding for a protein or polypeptide having fibronectin binding ability wherein said protein or polypeptide comprises at least one of the following amino acid sequences:
- __________________________________________________________________________Gly Gln Asn Ser Gly Asn Gln Ser Phe Glu Glu Asp Thr Glu GluAsp Lys Pro Lys Tyr Glu Gln Gly Gly Asn Ile Val Asp Ile AspPhe Asp Ser Val Pro Gln Ile His,Gly Gln Asn Lys Gly Asn Gln Ser Phe Glu Glu Asp Thr Glu LysAsp Lys Pro Lys Tyr Glu His Gly Gly Asn Ile Ile Asp Ile AspPhe Asp Ser Val Pro His Ile His, andGly Phe Asn Lys His Thr Glu Ile Ile Glu Glu Asp Thr Asn LysAsp Lys Pro Ser Tyr Gln Phe Gly Gly His Asn Ser Val Asp PheGlu Glu Asp Thr Leu Pro Lys Val.__________________________________________________________________________
- 7. A method for producing a fibronectin binding protein which comprises:
- a) transforming a suitable host cell with a hybrid-DNA-molecule of claim 6,
- b) harvesting the cells of (a) and culture medium of said cells and
- c) isolating the fibronectin binding protein from the cells and culture medium of (b) by a process which comprises fibronectin affinity chromatography followed by ion exchange chromatography.
- 8. The plasmid pFR001 having the deposit number DSM 4124.
- 9. A microorganism transformed by the plasmid of claim 8.
- 10. A plasmid pFR001 as contained in the E. coli strain 259 having the deposit number DSM 4124.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8702272-9 |
Jun 1987 |
SEX |
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Parent Case Info
This application is a continuation of application Ser. No. 07/746,087, filed Aug. 12, 1991, now abandoned, which is a continuation of application Ser. No. 07/201,028, filed Jun. 1, 1988, now abandoned.
Non-Patent Literature Citations (5)
Entry |
Flock, et al. EMBO J 6(8):2351-2357, 1987. |
Espersen, et. al. Infect & Immun 37(2):526-531, 1982. |
Ryden, et al. J. Biol. Chem 258(5):3396-3401, 1983. |
Abrahmsen, et al. Nucl. Acid. Res 14: 7487-7500, 1986. |
P.N.A.S. 80, 697-701, Feb. 1983, Lofdahl et al. Gene for Staphylococcoi Protein A. |
Continuations (2)
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Number |
Date |
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Parent |
746087 |
Aug 1991 |
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Parent |
201028 |
Jun 1988 |
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