Claims
- 1. An integral flat analytical element for use in the quantitative analysis of an analyte present in a liquid sample which comprises a flat container having a lid and a bottom, the lid having a sample introduction port therein, the lid, the bottom, or both being flexible and restorably deformable, said container comprising a reaction chamber and an observation chamber in liquid communication with each other, the reaction chamber containing a porous material which is elastomerically deformable positioned between the lid and bottom such that deformation of the lid, bottom, or both, causes deformation of the porous material, said observation chamber having at least two rigid transparent side wall portions opposed to each other in fixed positions to provide a predetermined light path.
- 2. The analytical instrument as claimed in claim 1, wherein a reagent is contained in said reaction chamber.
- 3. The analytical instrument as claimed in claim 1, wherein said reaction chamber and said observation chamber are positioned substantially on the same plane.
- 4. The analytical instrument as claimed in claim 1, wherein said reaction chamber and said observation chamber are superposed one on another.
- 5. A method for the optical measurement of an analyte in a liquid sample utilizing an integral flat analytical element which comprises a flat container having a lid and a bottom, the lid having a sample introduction port therein, the lid, the bottom, or both being flexible and restorably deformable, said container comprising a reaction chamber, and an observation chamber in liquid communication with each other, the reaction chamber containing a reagent which is reactive with the analyte, said observation chamber having at least two rigid transparent side wall portions opposed to each other in fixed positions to provide a predetermined light path, said reaction chamber containing a reagent which is reactive with said analyte; and
- introducing a liquid sample into the reaction chamber through the sample introduction port;
- repeatedly applying stress to the flexible bottom, lid, or both of the instrument to mix the liquid sample with the reagent and cause a reaction between the analyte in the liquid sample and the reagent in the reaction chamber; and
- photometrically observing a detectable change produced in the liquid by the reaction of the analyte and the reagent utilizing the transparent side wall portions of the observation chamber.
- 6. The method of claim 5 wherein the reaction chamber contains a porous material which is elastomerically deformable positioned between the lid and bottom such that deformation of the lid, bottom, or both, causes deformation of the porous material, and the reagent is contained in the porous material.
- 7. The method as claimed in claim 5, wherein said reaction chamber and said observation chamber are positioned substantially on the same plane.
- 8. The method as claimed in claim 5, wherein said reaction chamber and said observation chamber are superposed one on another.
Priority Claims (3)
Number |
Date |
Country |
Kind |
58-225187 |
Nov 1983 |
JPX |
|
58-225188 |
Nov 1983 |
JPX |
|
58-225189 |
Nov 1983 |
JPX |
|
BACKGROUND OF THE INVENTION
This is a continuation of application Ser. No. 676,054, filed Nov. 29, 1984 now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3690836 |
Buissiere et al. |
Sep 1972 |
|
3698822 |
Polanyi |
Oct 1972 |
|
4515889 |
Klose et al. |
May 1985 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0201837 |
Dec 1982 |
JPX |
Continuations (1)
|
Number |
Date |
Country |
Parent |
676054 |
Nov 1984 |
|