Claims
- 1. An isolated nucleic acid molecule comprising at least 15 consecutive nucleotides of a nucleic acid sequence selected from the group consisting of Seq. I.D. Nos. 1, 2, 3, 5, 6, 7, 9, 10, 11, 13, 14 and 15.
- 2. An isolated nucleic acid molecule according to claim 1 wherein the nucleic acid molecule includes at least 25 consecutive nucleotides of the specified nucleic acid sequence.
- 3. An isolated nucleic acid molecule according to claim 1 wherein the nucleic acid molecule includes at least 50 consecutive nucleotides of the specified nucleic acid sequence.
- 4. A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 1.
- 5. A recombinant nucleic acid molecule according to claim 4 wherein the nucleic acid molecule is arranged in antisense orientation relative to the promoter.
- 6. A cell transformed with a recombinant nucleic acid molecule according to claim 4.
- 7. A cell transformed with a recombinant nucleic acid molecule according to claim 5.
- 8. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 4.
- 9. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 5.
- 10. A transgenic plant according to claim 8 wherein the activity of at least one endogenous gene in the plant is modified as a result of the presence of the recombinant nucleic acid molecule.
- 11. A transgenic plant according to claim 10 wherein the plant is a Populus species and the affected endogenous gene is selected from the group consisting of PTD, PTLF, PTAG-1 and PTAG-2.
- 12. A transgenic plant according to claim 10 wherein the plant has a modified phenotype relative to non-transgenic plants of the same species.
- 13. A transgenic plant according to claim 12 wherein the modified phenotype is a modified fertility phenotype.
- 14. A transgenic plant comprising a recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises a promoter sequence operably linked to a first nucleic acid sequence, and wherein the promoter sequence is a promoter sequence from PTD, PTLF, PTAG-1 or PTAG-2.
- 15. A transgenic plant according to claim 14 wherein the first nucleic acid sequence encodes a cytotoxic polypeptide.
- 16. A transgenic plant according to claim 14 wherein the plant is a Populus species.
- 17. An isolated nucleic acid molecule comprising a nucleotide sequence of at least 50 nucleotides in length wherein said molecule shares at least 75% sequence identity with a nucleic acid selected from the group consisting of Seq. I.D. Nos. 1,2, 3,5, 6, 7,9, 10, 11, 13, 14 and 15.
- 18. An isolated nucleic acid molecule according to claim 17 wherein the molecule comprises a nucleotide sequence of at least 100 nucleotides in length and wherein said molecule shares at least 90% sequence identity with a nucleic acid selected from the group consisting of Seq. I.D. Nos. 1, 2, 3, 5, 6, 7, 9, 10, 11, 13, 14 and 15.
- 19. A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 17.
- 20. A recombinant nucleic acid molecule according to claim 19 wherein the nucleic acid molecule is arranged in antisense orientation relative to the promoter.
- 21. A cell transformed with a recombinant nucleic acid molecule according to claim 19.
- 22. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 19.
- 23. A purified protein having an amino acid sequence selected from the group consisting of:
(a) Seq. I.D. No. 4; (b) Seq. I.D. No. 8; (c) Seq. I.D. No. 12; (d) Seq. I.D. No. 16; and (e) sequences that differ from (a)-(d) by one or more conservative amino acid substitutions.
- 24. An isolated nucleic acid molecule encoding a protein according to claim 23.
- 25. An isolated nucleic acid molecule according to claim 24 wherein the nucleic acid molecule comprises a sequence selected from the group consisting of Seq. I.D. Nos. 1, 2, 3, 5, 6, 7, 9, 10, 11, 13, 14 and 15.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This is a continuation-in-part of U.S. patent application Ser. No. 09/287,700 filed Apr. 6, 1999 which claims the benefit of U.S. Provisional Application No. 60/080,851, filed Apr. 6, 1998, both of which applications are incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60080851 |
Apr 1998 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09410464 |
Oct 1999 |
US |
Child |
10104580 |
Mar 2002 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09287700 |
Apr 1999 |
US |
Child |
09410464 |
Oct 1999 |
US |