1. Field of the Invention
The present invention relates to a fluid mixing device used for a gene analysis and the like and, in particular, to a fluid mixing device constructed as a micro chip.
2. Description of the Related Art
In a field referred to as a μTAS (Total Analysis System), a flow channel and a storage tank are formed in a substrate made of glass or plastics (hereinafter referred to as “micro chip”) and a sample or a reagent is tested or analyzed by treating the sample or the reagent in the flow channel and the storage tank.
When the sample or the reagent is tested or analyzed by the use of the micro chip, the sample or the reagent is supplied to the flow channel and the storage tank of the micro chip from the outside of the micro chip. Further, the sample or the reagent supplied to the flow channel and the storage tank has pressure applied thereto from the outside of the micro chip, whereby the flow of the sample or the reagent is controlled and the treatment of stirring or mixing the sample or the reagent is performed.
In order to apply pressure to the sample or the reagent from the outside of the micro chip, the micro chip, a unit for supplying pressure for controlling an operation, and a unit for supplying a sample or a reagent are connected to each other by a tube or the like (see JP 2005-345463 A (patent document 1) and JP 2007-187616 A (patent document 2)).
As shown in
Furthermore, the micro chip is provided with first mixing tank flow channel 3a for feeding first liquid 1a from first supply tank 2a to mixing tank 9, and at a position different from a position where first mixing tank flow channel 3a is formed, the micro chip is provided with second mixing tank flow channel 3b connecting second supply tank 2b to mixing tank 9. First supply tank 2a and second supply tank 2b are connected respectively to a liquid supply unit (not shown) supplying liquid and a pressure supply unit (not shown) supplying pressure for controlling an operation through a tube.
When pressure applied to first liquid 1a in first supply tank 2a is increased, first liquid 1a is fed to mixing tank 9 through first mixing tank flow channel 3a. In the case where the amount of first liquid 1a that is fed is more than an allowance of mixing tank 9 or in the case where vibration is applied to the micro chip, first liquid 1a is fed to second supply tank 2b through second mixing tank flow channel 3b that is connected to mixing tank 9 and that is different from first mixing tank flow channel 3a. Second supply tank 2b is supplied with second liquid 1b, so that first liquid 1a is mixed with second liquid 1b in second supply tank 2b.
Depending on the purpose of the test or the analysis, there is a case where the mixing of first liquid 1a with second liquid 1b in a place other than mixing tank 9 is not allowed. Thus, second mixing tank flow channel 3b is provided with second valve 5b preventing first liquid 1a fed to mixing tank 9 from flowing toward second supply tank 2b through second mixing tank flow channel 3b.
Further, similarly, first mixing tank flow channel 3a is also provided with first valve 5a preventing second liquid 1b from flowing toward first supply tank 2a from mixing tank 9 through first mixing tank flow channel 3a. In this way, by appropriately providing respective mixing tank flow channels 3a, 3b with first valve 5a and second valve 5b, it is possible to prevent first liquid 1a from mixing with second liquid 1b in a place other than mixing tank 9.
As the valve preventing the flow from the mixing tank to the supply tank as described above, there is employed a passive valve utilizing flow resistance or a valve using a flexible material such as a diaphragm.
The passive valve utilizing flow resistance has a structure such that a flow channel is provided with a portion having a reduced cross-sectional area to make a flow resistance value very large, thereby making liquid flow only when the liquid is fed by pressure exceeding this flow resistance value (see JP 2003-190751 A (patent document 3) and JP 2006-142448 A (patent document 4)).
In the example of the related micro chip shown in
In the case where first liquid 1a is fed from first supply tank 2a to mixing tank 9 through first mixing tank flow channel 3a, pressure exceeding the flow resistance value of first valve 5a is applied to first liquid 1a in first supply tank 2a. As a result, first liquid 1a passes through first valve 5a. First liquid 1a is fed to mixing tank 9 in the state where the pressure of first liquid 1a is reduced by first valve 5a.
In the case where the amount of first liquid 1a that is fed exceeds the allowance of mixing tank 9, first liquid 1a is fed from mixing tank 9 toward second supply tank 2b for supplying second liquid 1b through second mixing tank flow channel 3b. When the pressure of first liquid 1a at second valve 5b is less than the flow resistance value of second valve 5b, first liquid 1a is stopped at second valve 5b. Thus, first liquid 1a is not fed from mixing tank 9 toward second supply tank 2b.
In the case where a passive valve is used as a valve preventing a flow from a mixing tank to a supply tank, the passage of liquid through the valve is controlled only by the pressure of the liquid. This eliminates the need for providing a physical force or an electric signal for opening or closing the valve from the outside of the micro chip, which results in providing an advantage of easing the handling of the micro chip.
In a valve using a flexible material such as a diaphragm, the flexible material is deformed by the physical force or the electric signal provided from the outside of the micro chip to thereby open or close a flow channel.
In the example of the related micro chip shown in
In the case where first liquid 1a is fed from first supply tank 2a to mixing tank 9 through first mixing thank flow channel 3a, first valve 5a is opened and second valve 5b is closed by a physical force or an electric signal provided from the outside of the micro chip. Thus, even in the case where the amount of first liquid 1a that is fed exceeds the allowance of mixing tank 9, the feed of first liquid 1a from mixing tank 9 to second supply tank 2b is stopped by second valve 5b. As a result, the feed of first liquid 1a to second supply tank 2b can be prevented.
In the case where the valve preventing the flow from the mixing tank to the supply tank is formed of a flexible material, the flow channel is opened or closed by deformation of the flexible material. This can reduce the possibility that the liquid leaks from the valve and hence can provide high reliability in the control of liquid (see JP 2003-139660 A (patent document 5) and JP 2003-139662 A (patent document 6)).
In the above example, description has been made on the assumption that as a method for controlling liquid in a supply tank, the feed of the liquid is not controlled but the pressure of the liquid is controlled. In addition to controlling the pressure of the liquid, controlling the liquid can be also performed by the amount of liquid that is fed by the use of a syringe pump or the like capable of controlling the amount of liquid that is fed in the supply tank. Even if the syringe pump is used, the amount of liquid that is fed and the capacities of the flow channel, the supply tank, and the mixing tank cannot be sufficiently controlled, so that it is surely thought that the amount of liquid that is fed to the mixing tank exceeds the allowance of the mixing tank to thereby cause the flow of the liquid from the mixing tank to the supply tank. Thus, even in the case where a flow rate is controlled by the use of the syringe pump or the like, a valve is required.
In the related micro chip shown in
In the case where the control of pressure to be applied to first liquid 1a does not satisfy a required accuracy, there is a possibility that the pressure of first liquid 1a is insufficient for the flow resistance value of first valve 5a or exceeds the flow resistance value of second valve 5b. In other words, there is a possibility that feeding the liquid to mixing tank 9 and preventing the flow of the liquid to the second supply tank 2 are not normally performed to thereby reduce the reliability of the micro chip.
Further, in the case where the valve that prevents the flow of the liquid from the mixing tank to the supply tank is constructed of a flexible material, there is a need to provide an external mechanism applying a force to the flexible material from the outside of the micro chip. Thus, the connection of the external mechanism to the micro chip is increased to thereby make the handling of the micro chip cumbersome, which may reduce productivity. Further, since the external mechanism is newly provided, there is also presented a problem in which the maintenance and the inspection of the external mechanism are newly required.
Therefore, the object of the present invention is to provide a fluid mixing device that includes a valve that prevents a flow from a mixing tank to a supply tank and that is not reduced in productivity and maintainability and is improved in operability and reliability even if high accuracy pressure control is not used.
In order to achieve the object described above, a fluid mixing device of the present invention includes a mixing tank, a plurality of supply tanks, mixing tank flow channels, valves, and branch flow channels. The mixing tank mixes at least two liquids. Each of the supply tanks is provided for each of the at least two liquids and supplies each liquid to the mixing tank. Each of the mixing tank flow channels connects each of the supply tanks to the mixing tank. Each of the valves is arranged in each of the mixing tank flow channels. Each of the branch flow channels is connected to each of the mixing tank flow channels and is connected from the one mixing tank flow channel to the valve in the other mixing tank flow channel. Further, when the valve has pressure applied thereto from the liquid having passed through the branch flow channel, the valve closes the mixing tank flow channel.
According to the construction described above, before the liquid passes through one mixing tank flow channel and is fed to the mixing tank, the liquid passes through the branch flow channel and is fed to the valve provided in the other mixing tank flow channel, and the valve closes the other mixing tank flow channel. Thus, this construction can prevent the liquid from flowing from the mixing tank to the other mixing tank flow channel.
The valve that prevents the flow from the mixing tank to the mixing tank flow channel is physically closed by the liquid, so that as compared with a passive valve using utilizing flow resistance, the valve can prevent the flow from the mixing tank to the mixing tank flow channel by a lower accuracy pressure control, which can improve operability and reliability. Further, the valve is opened or closed by the liquid in the fluid mixing device, so that it is not necessary to provide a mechanism operating the valve from the outside of the fluid mixing device, which can make it simple to connect the micro chip to an external mechanism and hence can improve productivity and maintainability.
According to the fluid mixing device of the present invention, the fluid mixing device includes a valve that prevents the flow from the mixing tank to the supply tank and is not reduced in productivity and maintainability and is improved in operability and reliability even if high accuracy pressure control is not used.
The above and other objects, features and advantages of the present invention will become apparent from the following description with reference to the accompanying drawings which illustrate examples of the present invention.
An exemplary embodiment of the present invention will be described in detail with reference to drawings.
Micro chip 10 has first supply tank 2a for supplying first liquid 1a and second supply tank 2b for supplying second liquid 1b. Further, micro chip 10 has first mixing tank flow channel 3a for feeding first liquid 1a from first supply tank 2a to mixing tank 9 and has second mixing tank flow channel 3b connecting second supply tank 2b to mixing tank 9 at a position different from a position where first mixing tank flow channel 3a is provided. First supply tank 2a is connected to a liquid supply unit (not shown) supplying first liquid 1a from the outside of micro chip 10 and to a pressure supply unit (not shown) that increases the pressure of first liquid 1a by a tube or the like. Similarly, second supply tank 2b is also connected to a pressure supply unit and the like.
Furthermore, in first mixing tank flow channel 3a is arranged first valve 5a that prevents a flow from mixing tank 9 to first supply tank 2a, and similarly, in the second mixing tank flow channel 3a is arranged second valve 5b that prevents a flow from mixing tank 9 to second supply tank 2b.
First valve 5a is connected to second branch flow channel 4b branched from second mixing tank flow channel 3b between second valve 5b and second supply tank 2b. First valve 5a is constructed in a closing structure by a flexible material such as a diaphragm. When first valve 5a has pressure applied thereto from second liquid 1b fed through second branch flow channel 4b, first valve 5a is deformed, whereby the communication between mixing tank 9 and first mixing tank flow channel 3a is blocked.
Similarly, second valve 5b is connected to first branch flow channel 4a branched from first mixing tank flow channel 3a between first valve 5a and first supply tank 2a. When first liquid 1a passes through first branch flow channel 4a and flows into second valve 5b, communication between mixing tank 9 and second mixing tank flow channel 3b is blocked.
Furthermore, first flow channel selection mechanism 6a that selects a flow channel through which a flowing-in liquid is made to pass by the pressure of the flowing-in liquid is arranged at a branch point between first mixing tank flow channel 3a and first branch flow channel 4a. Similarly, second flow channel selection mechanism 6b is arranged at a branch point between second mixing tank flow channel 3b and second branch flow channel 4b.
Here, the concrete construction of first flow channel selection mechanism 6a and second flow channel selection mechanism 6b will be described. The flow channel selection mechanism has one input flow channel corresponding to a flow channel portion that communicates with a supply tank side of a mixing tank flow channel and a plurality of output flow channels corresponding to flow channel portions that communicate with a mixing tank side of the mixing tank flow channel and with a branch flow channel, respectively. Further, the flow channel selection mechanism has passive valves arranged in the respective output flow channels, the passive valves being different form each other in flow resistance value.
Thus, if the pressure of liquid 11 fed to input flow channel 12 is less than the flow resistance value X, liquid 11 does not pass through passive valve 15 and passive valve 16. If liquid 11 has a pressure that is the flow resistance value X or more and that is less than the flow resistance value Y, liquid 11 does not pass through passive valve 16 but passes through only passive valve 15. Further, when the pressure of liquid 11 to be supplied is increased and then the pressure of supplied liquid 11 reaches the flow resistance value Y or more, the supplied liquid 11 passes through passive valve 15 and passive valve 16.
As shown in
Similarly, in second flow channel selection mechanism 6b, input flow channel 12 is connected to second supply tank 2b side of second mixing tank flow channel 3b. Output flow channel 13 including passive valve 15 having a flow resistance value Xb is connected to second branch flow channel 4b, and output flow channel 14 including passive valve 16 having a flow resistance value Yb larger than the flow resistance value Xb is connected to mixing tank 9 side of second mixing tank flow channel 3b.
In this regard, there is no need to make a difference between the flow resistance values Xa, Ya of first flow channel selection mechanism 6a and the flow resistance values Xb, Yb of second flow channel selection mechanism 6a.
Next, a method for mixing first liquid 1a with second liquid 1b in mixing tank 9 of micro chip 10 will be described.
First, a method for feeding first liquid 1a from first supply tank 2a to mixing tank 9 will be described. Here, it is assumed that the pressure of second liquid 1b in second supply tank 2b is not increased by a pressure supply unit arranged outside micro chip 10.
When the means of the pressure supply unit or the like that is provided outside micro chip 10 and that is connected to micro chip 10 by a tube or the like increases the pressure of first liquid 1a in first supply tank 2a, first liquid 1a passes through first mixing tank flow channel 3a from first supply tank 2a and reaches first flow channel selection mechanism 6a. The pressure of first liquid 1a in first flow channel selection mechanism 6a is less than the flow resistance value Xa, first liquid 1a remains in first flow channel selection mechanism 6a because the pressure of first liquid 1a is also less than the flow resistance value Ya.
Further, the pressure supply unit gradually increases the pressure of first liquid 1a in first supply tank 2a. When the pressure of first liquid 1a in first flow channel selection mechanism 6a reaches a value that is the flow resistance value Xa or more and that is less than the flow resistance value Ya, first liquid 1a flows only to first branch flow channel 4a side from first flow channel selection mechanism 6a and flows to second valve 5b.
Second valve 5b connected to first branch flow channel 4a is deformed by the pressure of first liquid 1a to thereby close second mixing tank flow channel 3b. Further, second valve 5b has a closing mechanism, so that first liquid 1a remains in second valve 5b.
After deformation of second valve 5b by the pressure of first liquid 1a is finished and then the interior of second valve 5b is brought into a saturated state by first liquid 1a, the pressure supply unit further increases the pressure of first liquid 1a in first supply tank 2a. As a result, the pressure of first liquid 1a in first flow channel selection mechanism 6a reaches the flow resistance value Ya. Thus, first liquid 1a remaining in first flow channel selection mechanism 6a starts to flow to first mixing tank flow channel 3a side toward mixing tank 9. Then, first liquid 1a reaches first valve 5a provided in first mixing tank flow channel 3a from first flow channel selection mechanism 6a.
First valve 5a is a valve closing first mixing tank flow channel 3a by the pressure of second liquid 1b, which is similar to second valve 5b. In the assumption described above, the pressure of second liquid 1b in second supply tank 2b is not increased, so that first valve 5a does not close first mixing tank flow channel 3a. Thus,
First liquid 1a passes through first valve 5a and reaches mixing tank 9.
When the amount of inflow of first liquid 1a becomes larger than the allowance of mixing tank 9 or vibration or the like is applied to micro chip 10 from the outside, first liquid 1a starts to flow from mixing tank 9 toward second supply tank 2b through second mixing tank flow channel 3b. Then, first liquid 1a in mixing tank 9 reaches second valve 5b provided in second mixing tank flow channel 3b.
In the process for feeding first liquid 1a to mixing tank 9, the pressure of first liquid 1a is increased, so that first liquid 1a flowing into second valve 5b works in such a way as to close second valve 5b. Thus, first liquid 1a in second mixing tank flow channel 3b remains in second valve 5b, so that first liquid 1a is not fed to second supply tank 2b.
When a sufficient amount of first liquid 1a is fed to mixing tank 9, the pressure supply unit decreases the pressure of first liquid 1a in first supply tank 2a.
In a state where the pressure of first liquid 1a is decreased to a value that is equal to or more than the flow resistance value Xa of first flow channel selection mechanism 6a and that is less than the flow resistance value Ya, first liquid 1a does not flow from first flow channel selection mechanism 6a to first mixing tank flow channel 3a but will continue to flow to first branch flow channel 4a. In other words, in this state, first liquid 1a continues to close second valve 5b in second mixing tank flow channel 3b.
When the pressure of first liquid 1a is decreased to a value equal to or less than the flow resistance value Xa of first flow channel selection mechanism 6a, first liquid 1a does not flow to first branch flow channel 4a from first flow channel selection mechanism 6a. Thus, second valve 5b opens second mixing tank flow channel 3b. In this way, the feeding of first liquid 1a to mixing tank 9 is finished.
In a case where first liquid 1a of the same amount as the allowance of mixing tank 9 remains in mixing tank 9, a portion of first liquid 1a in mixing tank 9 is discharged by another means, which will not be described here in detail, to thereby secure a space into which second liquid 1b different from first liquid 1a can flow.
Next, second liquid 1b in the second mixing tank 2b is fed to mixing tank 9. By the same procedure as a procedure for feeding first liquid 1a to mixing tank 9, second liquid 1b can be fed to mixing tank 9 without causing second liquid 1b or a mixture of first liquid 1a and second liquid 1b to flow from mixing tank 9 to first supply tank 2a.
Specifically, when the pressure of second liquid 1b in second supply tank 2b reaches a value that is equal to or more than the flow resistance value Xb of second flow channel selection mechanism 6b and that is less than the flow resistance value Yb, second liquid 1b is fed to first valve 5a through second branch flow channel 4b. As a result, first valve 5a closes first mixing tank flow channel 3a.
Thereafter, when the pressure of second liquid 1b is further increased and reaches more than the flow resistance value Yb of second flow channel selection mechanism 6b, second liquid 1b starts to flow from second flow channel selection mechanism 6b toward mixing tank 9 through second mixing tank flow channel 3b and reaches second valve 5b.
Second valve 5b is a valve that is closed by the pressure of first liquid 1a flowing into second valve 5b. The pressure of first liquid 1a is decreased when the feeding of first liquid 1a is finished, so that second valve 5b is opened. Thus, second liquid 1b having reached second valve 5b passes through second valve 5b and is fed to mixing tank 9. As a result, second liquid 1b is mixed with first liquid 1a for the first time in mixing tank 9.
In the case where second liquid 1b is mixed with first liquid 1a and then second liquid 1b is further fed to mixing tank 9, whereby the allowance of mixing tank 9 is exceeded, second liquid 1b or the mixture of second liquid 1b and first liquid 1a passes through first mixing tank flow channel 3a and flows toward first supply tank 2a. Since first valve 5a in first mixing tank flow channel 3a is closed, the mixture stops at first valve 5a.
When a sufficient amount of second liquid 1b is fed to mixing tank 9, the pressure of second liquid 1b is decreased to thereby finish feeding second liquid 1b.
As described above, in the exemplary embodiment of the present invention, first liquid 1a and second liquid 1b are fed to mixing tank 9 by the supply of pressure to first liquid 1a in first supply tank 2a and by the supply of pressure to second liquid 1b in second supply tank 2b, whereby first liquid 1a is mixed with second liquid 1b. Further, by increasing the pressure of first liquid 1a and the pressure of second liquid 1b, the valves preventing the flow of the liquid from the mixing tank to the supply tanks are physically closed. Thus, the exemplary embodiment of the present invention does not require a pressure control of high accuracy that controls the pressure of liquid to be fed to a range that is between the upper and lower limits of the flow resistance value of the valve, the pressure control being required in the case where a passive valve is used for a valve to prevent the flow of the liquid from the mixing tank to the supply tank.
Further, in the exemplary embodiment of the present invention, the valves that prevent the flow from the mixing tank to the supply tank are opened or closed by the pressures of first liquid 1a and second liquid 1b that flow in micro chip 10, so that an operation from the outside of the micro chip is not required in order to open or close the valves. Thus, there is no need to provide a mechanism for operating the valve outside the micro chip and hence a cumbersome operation is not required when a tube is connected to the micro chip. There is no need to provide a mechanism for operating the valve, so that maintenance and inspection work can be reduced.
According to the micro chip of the exemplary embodiment of the present invention, the micro chip is provided with valves that prevent the flow from the mixing tank to the supply tank, so that even if high accuracy pressure control of is not used, productivity and maintainability are not reduced and operability and reliability can be improved.
In micro chip 10 in
Each of the sheets is divided into portions that are bonded to the adjacent sheet and portions that are not bonded to the adjacent sheet. In
The respective constructions of the supply tank, the mixing tank flow channel, the flow channel selection mechanism, and the mixing tank that are formed on sheet 18 are the same as the respective constructions in the schematic view shown in
At an end portion on a side opposite to a side connected to first flow channel selection mechanism 6a of first branch flow channel 4a branched from first flow channel selection mechanism 6a, sheet 19 arranged in the center has first hole 7a cut out. Thus, liquid flowing through first branch flow channel 4a passes through first hole 7a and flows between sheet 19 and sheet 20. In other words, first hole 7a functions as a through hole.
Further, first branch flow channel 8a connecting first hole 7a to second valve 5b is formed between sheet 19 and sheet 20. Thus, first liquid 1a passes through first flow channel selection mechanism 6a, branch flow channel 4a, first hole 7a, and branch flow channel 8a and then reaches second valve 5b.
Similarly, second liquid 1b passes through second flow channel selection mechanism 6b, branch flow channel 4b, hole 7b, and branch flow channel 8b and then reaches first valve 5a for closing first mixing tank flow channel 3a. According to the construction described above, there can be realized a micro chip in which first branch flow channel 4a intersects second branch flow channel 4b, as shown in
In this regard, a non-flexible material can be also used for a portion of the three sheets 18, 19, and 20 in micro chip 10 shown in
Here, the specific construction of passive valve 15 and passive valve 16 in the flow channel selection mechanism in
As shown in
Further,
When
In
Further, in the description of the operation of the micro chip shown in
In the exemplary embodiment of the present invention, which is shown in
While preferred embodiments of the present invention have been described using specific terms, such description is for illustrative purposes only, and it is to be understood that changes and variations may be made without departing from the spirit or scope of the following claims.
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Number | Date | Country |
---|---|---|
2002-527250 | Aug 2002 | JP |
2003-139660 | May 2003 | JP |
2003-139662 | May 2003 | JP |
2003-190751 | Jul 2003 | JP |
2005-345463 | Dec 2005 | JP |
2006-142448 | Jun 2006 | JP |
2006-247533 | Sep 2006 | JP |
2006-247534 | Sep 2006 | JP |
2007-187616 | Jul 2007 | JP |
Number | Date | Country | |
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20130135960 A1 | May 2013 | US |