Claims
- 1. In a method for quantitating the amount of an analyte, if any, present in a test sample, in a fluorescence polarization immunoassay, the improvement comprising contacting the analyte, either in the clinical sample or a reagent mixture containing said analyte, with at least one compound selected from the group consisting of 1,10-phenanthroline, 8-hydroxy-7-iodo-5-quinolinesulfonic acid, naphthalene-1-sulfonic acid, and salts thereof, and any combination thereof, said compound being in an amount sufficient to reduce background interference from fluorescent substances endogenous to said sample.
- 2. The method of claim 1, wherein the test sample is a clinical sample selected from serum, plasma or urine.
- 3. The method of claim 2, wherein the selected compound is 1,10-phenanthroline.
- 4. The method of claim 2, wherein the selected compound is 8-hydroxy-7-iodo-5-quinolinesulfonic acid.
- 5. The method of claim 2, wherein the selected compounds in combination are naphthalene-1-sulfonic acid and 1,10-phenanthroline.
- 6. The method of claim 2, wherein the selected compounds in combination are naphthalene-1-sulfonic acid and 8-hydroxy-7-iodo-5-quinolinesulfonic acid.
- 7. In a fluorescence polarization immunoassay for a target analyte in a test sample, the improvement comprising reducing background interference by contacting the analyte-containing clinical sample or an analyte-containing reagent mixture with an effective amount of at least one compound selected from the group consisting of 1,10-phenanthroline, 8-hydroxy-7-iodo-5-quinolinesulfonic acid, and salts thereof.
- 8. A method of reducing interference in a fluorescence polarization immunoassay for a target analyte in a test sample comprising adding to said test sample or to a reagent mixture containing said test sample an effective amount of at least one compound selected from the group consisting of 1,10-phenanthroline, 8-hydroxy-7-iodo-5-quinolinesulfonic acid, naphthalene-1-sulfonic acid, and the salts thereof.
- 9. The method of claim 8 wherein the test sample is a clinical sample which is selected from serum, plasma or urine.
- 10. A set of reagents for the performance of a fluorescence polarization immunoassay for the quantitation of an analyte in a test sample, said reagents comprising a fluorescein-tagged analyte and antibody for said analyte, the improvement comprising the inclusion in said reagents of at least one compound selected from the group consisting of 1,10-phenanthroline, 8-hydroxy-7-iodo-5-quinolinesulfonic acid, and salts thereof and, optionally, naphthalene-1-sulfonic acid.
- 11. The set of reagents of claim 10, wherein the test sample is a clinical sample selected from serum, plasma, or urine.
- 12. The set of reagents of claim 11, wherein the selected compound is 1,10-phenanthroline.
- 13. The set of reagents of claim 11, wherein the selected compound is 8-hydroxy-7-iodo-5-quinolinesulfonic acid.
- 14. A set of reagents for the performance of a fluorescence polarization immunoassay, comprising:
- 1) lithium dodecyl sulfate in the range from about 0.5% to about 2%;
- 2) hydroxypropyl beta cyclodextrin in the range from about 10% to about 30%;
- 3) 1,10-phenanthroline in the range of from about 0.06% to about 0.25%; and
- 4) alkali metal azide in the range from about 0.09% to about 3.0%.
- 15. The set of reagents of claim 14 further comprising naphthalene-1-sulfonic acid in the range from about 0.75% to about 5%.
- 16. The set of reagents of claim 15 wherein the alkali metal azide, item 4), is sodium azide.
- 17. A reagent for a fluorescence polarization assay, said reagent comprising 0.85% naphthalene-1-sulfonic acid (alpha), 20% sodium salt of hydroxypropyl beta cyclodextrin, 0.20% 1,10-phenanthroline monohydrate, 1.25% dodecyl sulfate, lithium salt, and 0.09% sodium azide.
Parent Case Info
This application claims priority under 35 U.S.C. .sctn. 119(e) of provisional application Ser. No. 60/047,455, filed May 22, 1997.
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