Patent Application
20070134751
This application claims priority to EP 05023318.8, filed on Oct. 25, 2005, the disclosure of which is expressly incorporated herein by reference.
The invention relates to processes and devices for detecting an analyte in a sample by fluorescence measurement.
Measurement processes and measuring systems for biochemical analysis are important components of medical diagnostics. Analytes may be determined by measuring the light emitted by a fluorophore. The optimal choice of wavelength of the excitation light required for generating fluorescence plays an important part in making an accurate and reliable determination possible.
The excitation maxima of fluorophores are frequently within the ultraviolet spectral range (UV). Thus, for example, the longest-wavelength excitation maximum of NADH is at 340 nm. Currently, however, there are hardly any inexpensive, battery-powered light sources available for this spectral range, and even those are only in the near UV range.
Currently, light-emitting diodes of notable power (>0.1 mW), as the only inexpensive, narrow-band light source with low power consumption for excitation in the UV range, are industrially available only down to 365 nm, so that excitation can occur only far from the maximum of the excitation range. In addition to the accompanying loss of fluorescence signal, this gives rise to the problem of a very sensitive change in excitation efficiency as a function of the wavelength of the LED, since excitation takes place on the shoulder of the longest-wavelength absorbance peak. Thus, for example, the signal change to be expected for NADH is −5% per nm compared to excitation at 340 nm. In order to guarantee a technical signal stability of 1% for example, the wavelength of the LED would conversely have to remain stable within 0.2 nm, and this would be accomplished only with extreme complexity owing to power fluctuations, temperature dependence and ageing of the LED. Thus the requirement of sufficient wavelength stability would permit merely a very small interval for the allowed temperature range or, alternatively, necessitate incorporation of an active temperature control into a measuring system, but this would not be practicable owing to production costs and power consumption.
U.S. Pat. No. 4,547,465 describes a test element for analysing or transporting liquids, which comprises a porous zone consisting of a polymer with particulate material, for example pigments, dispersed therein. However, there is no indication whatsoever of an improvement in the accuracy of fluorescence measurements.
EP-A-0 066 648 relates to a multi-layer element for determining analytes in an aqueous medium, which element comprises a detection element with a detection layer and a reaction layer, the latter comprising a fibrous, porous and swellable medium. The element may furthermore have a light protection layer which contains particulate pigments. However, there is no indication whatsoever of an improvement in the accuracy of fluorescence determinations.
US 2002/0137027 relates to a process for determining hydrogen peroxide generated by an oxidase by means of a lanthanoid-ligand complex. Fluorescence is excited at a wavelength of preferably 330-415 nm and emission is detected at 600-630 nm.
U.S. Pat. No. 3,992,158 describes a test element for use in the analysis of liquids. The test element may contain one or more reflection layers which contain pigments such as titanium dioxide and barium sulphate, for example, as absorbers. This reflection layer is separated in space from the layer of the test element, which contains the detection reagents. There is furthermore no indication whatsoever of an improvement in the accuracy of fluorescence measurements.
The present invention provides a process for detecting an analyte by fluorescence measurement, the process having a reduced dependence of the measured signal on the excitation wavelength.
The solution according to the invention is to provide a process or system for detecting an analyte in a sample by fluorescence measurement of a fluorophore, wherein the detection medium which contains a fluorophore or a precursor of the fluorophore is admixed with an absorber whose absorbance spectrum superimposes the fluorescence excitation range of the fluorophore. The system consisting of the fluorophore and the absorber, which is produced in the detection medium, has an altered effective fluorescence excitation range with an altered fluorescence excitation maximum. Illumination with fluorescence excitation light can take place within the range of this altered excitation maximum. The measured signal obtained from determining fluorescence emission exhibits only low dependence on the wavelength of the excitation light. Owing to the altered wavelength of the excitation light, it is furthermore also possible to employ inexpensive light sources such as UV LEDs for example.
In a first aspect, the present invention relates to a process for detecting an analyte in a sample by fluorescence measurement, comprising the following steps:
In a further aspect, the invention relates to a test element for detecting an analyte, comprising
The above-mentioned features and aspects of the present invention will be further described and the invention will be better understood with reference to the following drawings.
The embodiments disclosed below are not intended to be exhaustive or to limit the invention to the precise forms disclosed in the following detailed description. Rather, the embodiments are chosen and described so that others skilled in the art may utilize their teachings.
According to the present invention the term “excitation maximum of the fluorophore” means the wavelength, at which a system consisting of the fluorophore in the absence of the absorber exhibits a maximum of fluorscence excitation. The term “excitation maximum of the system consisting of fluorophore and absorber” means the wavelength, at which a system consisting of a fluorophore and an absorber exhibits a maximum of fluorescence excitation. The term “effective excitation maximum” means the wavelength of the measured maximum of fluorescence excitation of a given system (fluorophore alone or fluorophore and absorber). According to the present invention, systems consisting of fluorophores and absorbers are employed, which exhibit an altered “effective excitation maximum”, i.e. an excitation maximum which has shifted compared to the excitation maximum of the fluorophore alone. An example for such a shift of the excitation maximum is shown in
The process and test element according to the invention may be employed for determining any analytes, for example in the field of clinical diagnostics. The analyte may be determined qualitatively and/or quantitatively. Preference is given to quantitative determination of the analyte, i.e. the amount, concentration or activity of the analyte in the sample to be examined is quantitatively determined by fluorescence measurement.
Analytes which may be determined by the process and test element according to the invention are any biological or chemical substances which can be detected by fluorescence measurement. If required, suitable detection regents may be employed here in the process or the test element, in addition to the fluorophore or the fluorophore precursor.
Preferably, the analyte is a substance determinable by one or more enzymatic reactions, for example an enzyme or an enzyme substrate. Preferred examples of the analyte are glucosedehydrogenase, lactate dehydrogenase, malate dehydrogenase, glycerol dehydrogenase, alcohol dehydrogenase, α-hydroxybutyrate dehydrogenase, sorbitol dehydrogenase, amino acid dehydrogenase, glucose, lactic acid, maleic acid, glycerol, alcohol, cholesterol, triglycerides, lipoproteins such as LDL or HDL, ascorbic acid, cysteine, glutathione, peptides, uric acid, urea, ammonium, salicylate, pyruvate, 5′-nucleotidase, creatine kinase (CK), lactate dehydrogenase (LDH) and carbon dioxide etc.
When detecting enzyme substrates, the detection reagents preferably contain one or more enzymes suitable for detecting the substrate. Examples of suitable enzymes are dehydrogenases selected from a glucose dehydrogenase (E.C.1.1.1.47), lactate dehydrogenase (E.C.1.1.1.27, 1.1.1.28), malate dehydrogenase (E.C.1.1.1.37), glycerol dehydrogenase (E.C.1.1.1.6), alcohol dehydrogenase (E.C.1.1.1.1) α-hydroxybutyrate dehydrogenase, sorbitol dehydrogenase or amino acid dehydrogenase, for example L-amino acid dehydrogenase (E.C.1.4.1.5). Further suitable enzymes are oxidases such as, for example, glucose oxidase (E.C.1.1.3.4) or cholesterol oxidase (E.C.1.1.3.6) and amino transferases such as, for example, aspartate or alanine amino transferase, 5′-nucleotidase or creatine kinase.
Particular preference is given to detecting glucose, the detection reagent comprising in particular glucose dehydrogenase.
When detecting enzymes, the detection reagents preferably contain one or more substrates suitable for detecting the enzyme.
Further components of detection reagents may be customary buffers, auxiliary substances or additives.
The starting material employed in the process or system according to the invention may be the fluorophore itself. Alternatively, it is possible to employ a fluorophore precursor from which a fluorophore whose fluorescence is then determined can be produced in the presence of the sample and the detection reagents.
The fluorophore is a substance which, when illuminated with fluorescence excitation light, produces a measured signal which indicates qualitatively the presence or absence of the analyte in the sample or which correlates with the amount, concentration or activity of the analyte in the sample. For example, the fluorophore itself may be the analyte to be determined or may be produced from the analytes to be determined. Preferably, however, the fluorophore is a substance which is a co-enzyme of an enzymatic reaction by which the analyte is determined. Preferred examples of co-enzymes are nicotin-adenine dinucleotides, such as NADH or NADPH, flavine nucleotides, etc.
Preference is given to using as a fluorophore a substance which has at least one excitation maximum in the UV range, such as NADH or NADPH, for example, or derivatives thereof. Suitable as fluorophores are of course also substances which have excitation maxima in the visible or near IR range.
Preferences is given to using as a fluorescence precursor a substance from which a fluorophore is produced, for example by a chemical reaction such as oxidation, for example. Preferred fluorophore precursors are substances from which fluorophores with at least one excitation maximum in the UV range can be produced, such as NAD or NADP for example or derivatives thereof.
According to the present invention, the detection medium which contains the fluorophore or fluorophore precursor and, where appropriate, at least one other detection reagent is admixed with an absorber whose absorbance/transmission properties for light illuminating in the detection medium change across the excitation range of the fluorophore. Preference is given to using an absorber which absorbs light across a part of the excitation range of the fluorophore and which is substantially transparent for light across another part of the excitation range of the fluorophore.
Particular preference is given to using an absorber which absorbs light within the shorter-wavelength part of the excitation range of the fluorophore and which is substantially transparent within the longer-wavelength part of the excitation range. This results in the effective excitation maximum of the fluorophore being shifted to a longer wavelength in the presence of the absorber. The excitation maximum is shifted preferably by at least 10 nm, particularly preferably by at least 20 nm and more preferably by at least 30 nm, based on the excitation maximum in the absence of the absorber.
Preference is given in the process according to the invention to illuminating with light for excitation of the fluorophore in the range of the altered effective excitation maximum, for example in a range of ±10 nm, in particular ±5 nm, around the wavelength in the excitation maximum of the altered effective excitation range. Thus, when using NADH or NADPH as fluorophore, for example, fluorescence excitation is at a wavelength in the range of preferably 360 nm or higher, in particular 365-380 nm. Fluorescence excitation is carried out using a suitable light source, for example a halogen lamp, a light-emitting diode or a laser diode. Preference is given to light-emitting or laser diodes which give off light in a wavelength range of 370-390 nm. In this way it is possible to use inexpensive light sources for fluorescence excitation.
In order to enable the excitation maximum of the fluorophore to be shifted as efficiently as possible, use is advantageously made of an absorber which changes relative transmission in the detection medium for incident light across the excitation range of the fluorophore from no more than 20%, preferably no more than 10%, to at least 80% and preferably at least 90%, based on maximum transmission in the detection medium used (transmission in the absence of the absorber). Relative transmission of the detection medium is changed here preferably within a wavelength range of ≦100 nm, particularly preferably ≦60 nm and most preferably ≦40 nm.
Suitable absorbers are any substances which absorb light across a part of the excitation range of the fluorophore and whose presence does not interfere with the detection process.
The absorber is preferably in the form of particles which have a diameter of ≦1 μm, preferably ≦500 nm and particularly preferably of 200-400 nm. The particle size is preferably at least 50 nm. Preferred examples of suitable absorber materials are metal oxides and metal salts such as metal sulphides or metal sulphates for example, in particular oxides of titanium, such as TiO, TiO2, oxides of zirconium, such as ZrO2, oxides or sulphides of zinc, such as ZnO or ZnS, and barium salts such as, for example, BaS or BaSO4, and any combinations thereof. The absorber particularly preferably contains TiO2 which may be in the form of rutile, for example. In principle, pigments which are employed as UV blockers in sun protection creams or other formulations are also suitable for the process according to the invention.
Preference may also be given to using absorber materials which have light-scattering properties so that the fluorescence excitation light is scattered several times in the area of the detection medium and the average path length of the excitation light in the detection medium is increased in order to obtain more efficient excitation.
By varying the absorber material, grain size, crystal structure or/and purity, in particular by adding relatively small amounts of further absorbers, it is possible to vary the position and shape of the absorbance spectrum and thereby also the shape and position of the excitation range of the system consisting of the fluorophore and the absorber.
A suitable choice of fluorophore or/and absorber enables the slope of the shoulder of the effective excitation range of the system of the fluorophore and the absorber to be varied at the desired wavelength. Thus it is possible, for example, by using ZrO2 as absorber, to shift the absorbance shoulder to shorter wavelengths compared to TiO2. This enables the amplitude of the effective excitation spectrum of fluorophore and absorber to be increased at the desired wavelength, it nevertheless being possible for the slope of the shoulder at this point to be brought within a tolerable range. Thus
The fluorescence emission of the fluorophore can be determined in the usual way at one or more suitable measuring wavelengths by using suitable detection systems known to the skilled worker. Said determination may thus also be carried out by measuring fluorescence quenching due to, for example, the presence of the analyte.
The process according to the invention can markedly reduce the dependence of the measured signal on the wavelength of the fluorescence excitation light. Preference is given to achieving a signal stability of ≦1% per nm of change in the fluorescence excitation wavelength.
The process may be carried out in the form of a liquid assay, it being possible for the fluorophore or fluorophore precursor, where appropriate at least one further reagent and the absorber to be present in the form of a suspension in an aqueous or non-aqueous liquid or as a powder.
Preference is given to carrying out the process as a dry assay, with the reagent being applied to a test element. The test element may comprise, for example, a test strip or a test tape of absorbent or/and swellable material, to which the sample to be examined is applied. Suitable materials may be selected, for example, from the group of celluloses, plastic materials etc. Other preferred examples of test elements are integrated measuring systems, for example those which comprise a sampling element, such as a needle or lancet, integrated in measuring equipment and, where appropriate, equipment for sample transport. The test element may have one or more layers comprising the detection reagents, the absorber and the fluorophore or fluorophore precursor. Preference is given here to the fluorophore or fluorophore precursor and the absorber being arranged on the test element in such a way that incident light for excitation of the fluorophore first hits the absorber and then the fluorophore or said fluorophore and absorber at the same time. Preference is given to arranging the fluorophore or fluorophore precursor and the absorber in one layer on the test element. EP-A-1035920 describes preferred test strips. EP-A-1424040 describes preferred examples of designing the test element as a test tape, i.e. as a test element which comprises a variety of test strips, with WO 03/009 759 and WO 2004/107 970 disclosing preferred examples of integrated measuring systems. Alternatively, the detection reagent may also be embedded in a gel matrix (see, for example, DE 102 21 845). Reference is explicitly made to the disclosure of the abovementioned documents. Each of EP-A-1035920, EP-A-1424040, WO 03/009759, and WO 2004/107970 are hereby expressly incorporated herein by reference. Particular preference is given to a procedure in which the fluorophore and the absorber together are present in one phase or one layer, for example on a test element, prior to applying the sample.
The sample to be examined is usually a liquid sample, in particular a body fluid such as blood, plasma, serum, saliva or urine. Particular preference is given to determining glucose in blood.
The invention furthermore relates to a novel test element for detecting an analyte, which comprises a fluorophore, an absorber and, where appropriate, detection reagents, with these components being arranged on the test element in such a way that incident light for excitation of the fluorophore first hits the absorber and then the fluorophore or hits the fluorophore and absorber essentially at the same time. Preference is given to arranging said components in such a way that they are present in one phase or one layer on the test element prior to applying the sample.
The test element is preferably designed in the form of a test strip, test tape or integrated measuring system. It may be employed in a process for detecting an analyte in a sample, which comprises the steps:
A further subject matter still is the use of an absorber, as explained above, in a test element for modifying the fluorescence excitation maximum of a fluorophore, in particular in a process for determining analytes in a sample.
While this invention has been described as having an exemplary design, the present invention may be further modified within the spirit and scope of this disclosure. This application is therefore intended to cover any variations, uses, or adaptations of the invention using its general principles. Further, this application is intended to cover such departures from the present disclosure as come within known or customary practice in the art to which this invention pertains.
| Number | Date | Country | Kind |
|---|---|---|---|
| 05 023 318.8 | Oct 2005 | EP | regional |
