FLUORESCENT ACTIVATED CELL SORTING FOR ENZYME SCREENING

Information

  • Research Project
  • 2540800
  • ApplicationId
    2540800
  • Core Project Number
    R43GM057135
  • Full Project Number
    1R43GM057135-01
  • Serial Number
    57135
  • FOA Number
  • Sub Project Id
  • Project Start Date
    11/1/1997 - 27 years ago
  • Project End Date
    4/30/1998 - 26 years ago
  • Program Officer Name
  • Budget Start Date
    11/1/1997 - 27 years ago
  • Budget End Date
    4/30/1998 - 26 years ago
  • Fiscal Year
    1997
  • Support Year
    1
  • Suffix
  • Award Notice Date
    9/30/1997 - 27 years ago
Organizations

FLUORESCENT ACTIVATED CELL SORTING FOR ENZYME SCREENING

There has been increasing demand in the research reagent, diagnostic reagent and chemical process industries for protein-based catalysts possessing novel capabilities. At present, this need is largely addressed using enzymes purified from a variety of cultivated microorganisms, generally members of the domains Bacteria and Archaea. However, because less than 1% of naturally occurring microorganisms have been grown in culture, alternative techniques must be developed to exploit the full breadth of diversity for potentially valuable new products. We propose to develop fluorescent activated cell sorting for an extreme high throughput screening for recombinant expression clones from uncultivated organisms to discover a large number of unique, robust, specific biocatalysts for the cost effective synthesis of chiral synthons for use in production of pharmaceuticals, biological intermediates and natural products. PROPOSED COMMERCIAL APPLICATION: and chemical process industries has already begun to grow. Growth in these fields is limited by a lack of enzyme activities useful under the processing conditions required. RBI currently provides a number of enzyme libraries for various enzyme classes (Glycosidases; Esterases/Lipases; Aminotransferases; Phosphatases) suitable for screening. This project will serve to increase the number of enzymes available from RBI with which researchers and process engineers can design new methods.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG3
  • Study Section Name
  • Organization Name
    DIVERSA CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    SAN DIEGO
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    921213829
  • Organization District
    UNITED STATES