Claims
- 1. A fluorescent magnesium indicator composition comprising a substituted carboxy-quinolizine bound selectively to magnesium, wherein said binding has a dissociation constant between about 0.4 mM to about 5.0 mM.
- 2. The composition of claim 1, wherein said carboxy-quinolizine is a substituted 4-oxo-4H-quinolizine-3-carboxylic acid.
- 3. The composition of claim 1, wherein said carboxy-quinolizine is substituted with a functional group at the C-1 position.
- 4. The composition of claim 3, wherein said functional group at said C-1 position is selected from the group consisting of bromide, —N(CH2CO2Me)2, —CH2CH(CO2Et)2, and —CH═C—(CO2Et)2.
- 5. The composition of claim 1, wherein said carboxy-quinolizine is substituted with a functional group at the C-1 and C-8 positions.
- 6. The composition of claim 3, wherein said functional group at said C-1 position is bromide.
- 7. A composition suitable for selective fluorescence detection of magnesium, comprising at least one substituted carboxy-quinolizine, wherein said carboxy-quinolizine is substituted with a functional group at the C-1 and C-8 positions and said functional group at said C-8position is selected from the group consisting of —C(CO2Me2)(CO2Et), —CH2CO2H and —CH(CH2CO2H)(CO2H).
- 8. A composition suitable for selective fluorescence detection of magnesium, comprising at least one substituted carboxy-quinolizine, wherein said carboxy-quinolizine is substituted with a functional group at the C-1 and C-8 positions and said functional group at said C-1 position is bromide, and said functional group at said C-8 position is —C(CO2Me2)(CO2Et).
- 9. The composition of claim 1, wherein said carboxy-quinolizine comprises at least two carboxyl groups.
- 10. The composition of claim 1, wherein said carboxy-quinolizine comprises a triacid.
- 11. The composition of cl wherein said carboxy-quinolizine has a dissociation constant of about 1 mM.
- 12. The composition of claim 1, wherein said substituted carboxy-quinolizine is selected from the group consisting of 4-oxo-8-chloro-4H-quinolizine-3-carboxylic acid, 4-oxo-8-chloro-4H-quinolizine-3-carboxylic acid, [6,7]-benzo-4-oxo-4H-quinolizine-3-carboxylic acid, 1-bromo-4-oxo-4H-quinolizine-3-carboxylic acid, 1-[N,N-di(carboxymethyl)]-4-oxo-4H-quinolizine-3-carboxylic acid, 1-(2,2-dicarboxyvinyl)-4-oxo-4H-quinolizine-3-carboxylic acid, 1-(2,2-dicarboxyethyl)-4-oxo-4H-quinolizine-3-carboxylic acid, 8-amino-4-oxo-4H-quinolizine-3-carboxylic acid, 8-[3-N,N-di(carboxymethyl)phenoxy]-4-oxo-4H-quinolizine-3-carboxylic acid, 8-carboxymethyl-4-oxo-4H-quinolizine-3-carboxylic acid, 8-(1,2-dicarboxyethyl)-4-oxo4H-quinolizine-3-carboxylic acid, 8-(4-methoxyphenyl)-4-oxo-4H-quinolizine-3-carboxylic acid, 8-(naphtha-1yl)-4-oxo-4H-quinolizine-3-carboxylic acid, and 8-(benzo[b]furyl)-4-oxo-4H-quinolizine-3-carboxylic acid.
- 13. A diagnostic reagent suitable for use for monitoring of physiological magnesium levels, said diagnostic reagent comprising a substituted carboxy-quinolizine bound selectively to magnesium, wherein said binding has a dissociation constant between about 0.4 mM to about 5.0 mM, and fluoresces upon binding to said magnesium.
- 14. The diagnostic reagent of claim 13 for monitoring of the magnesium levels of a patient suffering from or suspected of suffering from cardiovascular disease or hypertension.
Parent Case Info
The present application claims benefit of U.S. Provisional Patent Application Ser. No. 60/191,862, filed on Mar. 24, 2000.
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|
Number |
Date |
Country |
|
60/191862 |
Mar 2000 |
US |