Claims
- 1. A fluorescent probe which comprises a detectably labeled marker component linked to one member of a specific binding pair wherein said detectably marker component comprises a fluorophore moiety which is linked to one or more solubilizing polyoxyhydrocarbyl moieties and wherein said fluorescent probe has diminished non-specific binding to biological macromolecules compared with a fluorescent probe comprising said member of a specific binding pair linked to said fluorophore moiety, but not linked to said polyoxyhydrocarbyl moieties.
- 2. The fluorescent probe of claim 1 wherein said a detectably labelled marker component which comprises a fluorophore moiety which is linked to at least one solubilizing polyoxyhydrocarbyl moiety where said solubilizing polyoxyhydrocarbyl moiety is a water soluble carbohydrate, of a water soluble carbohydrate derivative.
- 3. The fluorescent probe of claim 2 wherein said marker component has two or more solubilizing moieties.
- 4. The fluorescent probe of claim 3 wherein said marker component has about two to about six solubilizing moieties per fluorophore moiety.
- 5. The fluorescent probe of claim 4, wherein said fluorophore moiety comprises a luminescent substantially planar molecular structure.
- 6. The fluorescent probe of claim 5, wherein said fluorophore moiety comprises a substantially planar conjugated electron system.
- 7. The fluorescent probe of claim 6, wherein said fluorophore moiety comprises a nitrogen containing macrocycle.
- 8. The fluorescent probe of claim 7 wherein said macrocycle comprises a porphyrin derivative wherein one or more bridging carbon atoms has been replaced by nitrogen.
- 9. The fluorescent probe of claim 8 wherein the solubilizing moiety comprises polyethyleneglycol or a polyethyleneglycol derivative.
- 10. The fluorescent probe of claim 9 wherein said solubilizing moiety has a molecular weight of from about 200 to about 20,000.
- 11. The fluorescent probe of claim 9 wherein said macrocycle is coordinated to a central atom.
- 12. The fluorescent probe of claim 11 wherein the central atom coordinates a solubilizing moiety as an axial ligand.
- 13. The fluorescent probe of claim 12 wherein said macrocycle comprises a porphyrin derivative wherein from 1 to 4 of the bridging carbon atoms is replaced by nitrogen.
- 14. The fluorescent probe of claim 13 wherein said macrocycle comprises a phthalocyanine derivative.
- 15. The fluorescent probe of claim 14 wherein said central atom comprises silicon.
- 16. The fluorescent probe of claim 13 wherein said macrocycle has a low degree of symmetry so as to enhance the polarization of emission parallel to polarization of absorption.
- 17. The fluorescent probe of claim 13 wherein said macrocycle has a lower symmetry than D.sub.4h.
- 18. The fluorescent probe of claim 17 wherein said macrocycle comprises a porphyrin derivative wherein 1 to 4 of the bridging carbons is replaced by nitrogen.
- 19. The fluorescent probe of claim 18 wherein said macrocycle has at least one fused aromatic ring.
- 20. The fluorescent probe of claim 18 wherein said macrocycle comprises a derivative of monoazaporphyrin, diazaporphyrin or triazaporphyrin.
- 21. The fluorescent probe of claim 20 wherein said macrocycle has at least one fused aromatic ring.
- 22. The fluorescent probe of claim 17 wherein said macrocycle comprises a porphyin derivative having at least one fused aromatic ring and wherein optionally 1 to 4 of the bridging carbons is replaced by nitrogen.
- 23. The fluorescent probe of claim 2 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 24. The fluorescent probe of claim 3 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 25. The fluorescent probe of claim 4 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 26. The fluorescent probe of claim 5 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 27. The fluorescent probe of claim 6 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 28. The fluorescent probe of claim 7 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 29. The fluorescent probe of claim 8 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 30. The fluorescent probe of claim 9 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 31. The fluorescent probe of claim 10 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 32. The fluorescent probe of claim 11 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 33. The fluorescent probe of claim 12 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 34. The fluorescent probe of claim 13 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 35. The fluorescent probe of claim 14 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 36. The fluorescent probe of claim 15 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 37. The fluorescent probe of claim 16 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 38. The fluorescent probe of claim 17 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 39. The fluorescent probe of claim 18 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 40. The fluorescent probe of claim 19 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 41. The fluorescent probe of claim 20 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 42. The fluorescent probe of claim 21 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 43. The fluorescent probe of claim 22 wherein said member of a specific binding pair has at least one sterically tolerant marker component attachment site capable of permitting said probe to form a specific binding pair.
- 44. The fluorescent probe of claim 1 wherein said biological macromolecule is human serum albumin.
- 45. The fluorescent probe of claim 7 wherein said macrocycle comprises an azaporphyrin derivative wherein one or more bridging carbon atoms has been replaced by nitrogen.
- 46. The fluorescent probe of claim 7 wherein said macrocycle comprises a corrin derivative.
- 47. The fluorescent probe of claim 7 wherein said macrocycle comprises a sapphyrin derivative.
Parent Case Info
This is a divisional of U.S. patent application Ser. No. 07/701,449, filed May 15, 1991, now U.S. Pat. No. 5,403,928, which is a Continuation-in-Part application of U.S. patent application Ser. No. 07/523,601 filed May 15, 1990, now abandoned.
Foreign Referenced Citations (1)
Number |
Date |
Country |
8804777 |
Jun 1988 |
EPX |
Divisions (1)
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Number |
Date |
Country |
Parent |
701449 |
May 1991 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
523601 |
May 1990 |
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