Patent Application
20230104281
Modern super-resolution fluorescence microscopy techniques permit observation of biological processes in living organisms down to molecular level (Sahl, S. J.; Hell, S. W.; Jakobs, S., Nat. Rev. Mol. Cell. Biol. 2017, 18 (11), 685-701; Hell, S. W, Angew Chem Int Ed Engl 2015, 54 (28), 8054-66). Success of imaging experiments depends on the availability of biocompatible fluorescent probes for the specific labelling of cellular structures. Usually, these probes are constructed by coupling a fluorescent dye to a small molecule ligand via a linker to target a protein of interest. A linker and a fluorophore make significant contributions to the final properties of such probes often resulting in compromised cell permeability and off-targeting (Wang, L.; Frei, M. S.; Salim, A.; Johnsson, K., J Am Chem Soc 2019, 141 (7), 2770-2781). The ideal biocompatible fluorophore fulfil at least the following five criteria: First, the fluorescent dye should be highly photostable avoiding rapid bleaching in the course of an experiment. Second, it should have high extinction coefficient and quantum yield that results in a good brightness. Third, a derivatization of the fluorescent dye molecule with (i) ligands that specifically bind to other (bio)molecules in vitro or in vivo or, (ii) reactive groups such as activated esters (iii) molecules that can control the fluorescence properties of the fluorophore should be possible. Fourth, it should not interact with biomolecules present in the cell and show minimal background. Fifth, the fluorophore should be membrane permeable. Numerous fluorophores exist that fulfil the first three criteria, the tetramethylrhodamine (TMR) being an example of fluorophore whose derivatives show relatively low permeability and high off-targeting. Only few fluorophores available that also fulfil the fifth criterion. Recently several groups introduced fluorophores that show minimal background binding or are fluorogenic and are able to mask this unspecific binding. These fluorophores are various derivatives of rhodamines. The most prominent examples are carbopyronines (CP) (Butkevich A. N.; Mitronova G. Y.; Sidenstein. S. C.; Klocke J. L.; Kamin D.; Meineke D. N. H.; D'Este E.; Kraemer P. T.; Danzl J. G.; Belov V. N.; Hell S. W., Angew. Chem. Int. Ed. 2016, 55, 3290-3294) and silicon-rhodamines (SiR) (Figure 1) (Lukinavic̆ius G; Umezawa K; Olivier N; Honigmann A; Yang G; Plass T; Mueller V; Reymond L; Correa IR Jr; Luo ZG; Schultz C; Lemke EA; Heppenstall P; Eggeling C; Manley S; Johnsson K, Nat Chem, 2013, 5(2), 132-139).
A lot of interest is dedicated to the rhodamine class fluorophores, which are able to cycle between non-fluorescent spirolactone and a fluorescent zwitterion forms (Scheme 2). This property is exploited to generate fluorogenic probes, which are sensitive to number of environmental factors: pH, ion concentration, enzyme, tension force, local microenvironment polarity or light (Uno, S. N.; Tiwari, D. K.; Kamiya, M.; Arai, Y.; Nagai, T.; Urano, Y., Microscopy (Oxf) 2015, 64 (4), 263-77). Because spirolactone is a more hydrophobic molecule than zwitterion, the change of the equilibrium between these two forms contributes to the permeability of the rhodamine-based fluorescent probes (Lukinavic̆ius, G.; Reymond, L.; Umezawa, K.; Sallin, O.; D'Este, E.; Gottfert, F.; Ta, H.; Hell, S. W.; Urano, Y.; Johnsson, K., J. Am. Chem. Soc. 2016, 138 (30), 9365-8.
It is assumed that regular rhodamines and carbon-substituted analogs (carbopyronines) have this equilibrium shifted towards zwitterion form, resulting in relatively poor permeability. Several studies attempted to induce spirolactone form preference by introduction of the electron-withdrawing groups in the xanthene core (Zheng, Q.; Ayala, A. X.; Chung, I.; Weigel, A. V.; Ranjan, A.; Falco, N.; Grimm, J. B.; Tkachuk, A. N.; Wu, C.; Lippincott-Schwartz, J.; Singer, R. H.; Lavis, L. D., ACS Cent Sci 2019, 5 (9), 1602-1613) or in the benzoic acid substituent (Wang, L.; Tran, M.; D'Este, E.; Roberti, J.; Koch, B.; Xue, L.; Johnsson, K., bioRxiv 2019, 690867). However, all these approaches result in a bulkier core structure and alter physicochemical properties of the dyes.
The marking of proteins and nucleic acids with fluorescent dyes in living cells represents a widely used technique in life sciences and medical research. Multiple strategies have been developed to reach this goal. The most frequently used methods are: the tetracysteine tag that binds to biarsenical fluorophores, the SNAP/CLIP-tag that irreversibly reacts with benzylguanine (BG) or benzylcytosine (BC) derivatives (Keppler, A.; Gendreizig, S.; Gronemeyer, T.; Pick, H.; Vogel, H.; Johnsson, K., Nat Biotechnol 2003, 21 (1), 86-9), the Halo-tag that reacts with primary chlorides and dihydrofolate reductase that binds to trimethoprim derivatives (Hinner, M. J.; Johnsson, K., Curr Opin Biotechnol 2010, 21 (6), 766-76). Alternatively, a specific fluorescence labelling of a protein of interest can be achieved through the incorporation of an unnatural, fluorescent amino acid (Liu, C. C.; Schultz, P. G., Annu Rev Biochem 2010, 79, 413-44). Finally, proteins, DNA or RNA can be labelled using specifically binding ligands. The final probe is composed of the targeting moiety, linker and fluorescent dye. All these components contribute to the final properties of the probe. One on the most important property is cell membrane permeability, which allows efficient delivery of fluorophore to the target. This problem is often overcome by delivering fluorophore into the cell through invasive methods such as microinjection (Keppler, A.; Arrivoli, C.; Sironi, L.; Ellenberg, J., Biotechniques 2006, 41 (2), 167-70, 172, 174-5), bead-loading (Maurel, D.; Banala, S.; Laroche, T.; Johnsson, K., ACS Chem Biol 2010, 5 (5), 507-16) or electroporation (Jones, S. A.; Shim, S. H.; He, J.; Zhuang, X., Nat Methods 2011, 8 (6), 499-508).
Kocisova et al., in Journal of Molecular Structure, vol. 651-653 (2003), pages 115-122, disclose a carboxytetramethylrhodamine conjugate which has been prepared by using the commercial available 5-carboxytetramethylrhodamine N-hydroxysuccinimide ester (see page 117, left column, lines 2-5) and, thus, represents a 5-carboxytetramethylrhodamine conjugate.
Clerc et al., in Chem Bio Chem, vol. 10, no. 16, (2009), pages 2638-2643, disclose a rhodamine-tagged Syringolin A (SyIA) derivative which has been prepared by using a solution of a commercial available mixture of 5- and 6-rhodamine amine (see page 2642, left column, under synthesis of Rh-SyIA) and, thus, represents a 5/6-rhodamine conjugate.
Jolimaitre et al., in Chemistry and Physics of Lipids, vol. 133, no. 2 (2005), pages 215-223, disclose a tetramethylcarboxyrhodamine conjugate which has been prepared by reacting the N-amine of Lysine with the activated hydroxybenzotriazolyl ester of 5,6-tetramethylcarboxyrhodamine (see page 218, right column, third paragraph) and, thus, represents a 5,6-tetramethylcarboxyrhodamine conjugate.
Gee et al., in Bioorganic & Medicinal Chemistry Letters, vol. 11, no. 16, (2001), pages 2181-2183, disclose a caged Q-rhodamine dextran conjugate which has been prepared by using bisacylated 5- and 6-carboxy-Q-rhodamine (see page 2181, right column, second paragraph) and, thus, represents a mixture of 5/6-carboxyrhodamine isomers.
However, such 5′- and 6′-isomers of rhodamine are not able to provide a neighbouring group effect like the rhodamine 4′ isomers of the present invention.
In view of the drawbacks of fluorescent dyes of the prior art and the alternative strategies to obviate the limitations of their delivery to living cells and tissues, the main object of the present invention is to provide improved fluorescent dyes, in particular fluorescent dyes with enhanced cell permeability while still exhibiting desirable optical characteristics such as high photostability, high extinction coefficients and high quantum yields.
This objective has been achieved by providing novel compounds and fluorescent dyes which are rhodamine 4′ isomers according to claims 1-11, conjugates comprising said compounds and dyes according to claim 12, as well as the applications of the claimed compounds according to claims 13 to 22.
The novel fluorescent dyes of the invention are rhodamine 4′-isomers that have the following general structural formula A
wherein
R1, R2, R3, R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), N3, NH2, NH(alkyl), N(alkyl)2, NH(aryl), NH(aryl)2, NO2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl) COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), C(O)N(alkyl)2, C(O)N(aryl)2, P(O)OH(alkyl), P(O)OH(aryl), P(O)(—O-alkyl)2, P(O)(—O-aryl)2, PO3H2, SO3H, alkyl, substituted alkyl, alkenyl, substituted alkenyl;
R2 and R8 or R7 and R8 taken together may form a cyclic structure;
R5 and R9 or R9 and R10 taken together may form a cyclic structure;
R3 and R7 or R4 and R9 taken together may form a cyclic structure;
R1 and R2 or R5 and R6 taken together may form a cyclic structure;
X is selected from CR14R15, a heteroatom, in particular O, S, NR14, SO2, P(O)OH, P(O)OR14, SiR14R15, GeR14R15, where R14 and R15 are alkyl or aryl;
Z is selected from O(alkyl), O(aryl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), S(O)2(—O-alkyl), S(O)2(—O-aryl), S(O)2NH(alkyl), S(O)2NH(aryl), S(O)2N(alkyl)2, S(O)2N(aryl)2, S(O)2N(alkyl)(aryl),, C(O)O(alkyl), C(O)O(aryl), C(O)(alkyl), C(O)(aryl), P(O)OH(—NH-alkyl), P(O)OH(—O-alkyl), P(O)OH(—NH-aryl), P(O)OH(—O-aryl), P(O)(—O-alkyl)2, P(O)(—NH-alkyl)2, P(O)OH(—N(alkyl)2), P(O)OH(—N(aryl)2), P(O)(—N(aryl)2)2, P(O)(—N(alkyl)2)(—N(alkyl)2), P(O)(—O-aryl)2, P(O)(—NH-aryl)2, P(O)(—O-alkyl)(-O-aryl), P(O)(—NH-alkyl)(-O-aryl), P(O)(—O-alkyl)(-NH-aryl), P(O)(—NH-alkyl)(-NH-aryl), C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, in particular C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, CON(aryl)2, C(O)O(alkyl) and C(O)O(aryl), or any group which induces a neighboring group effect via steric, ionic or bonding interactions with the adjacent carboxyl group involved in spirolactone formation resulting in a shift of the equilibrium between zwitterionic form and spirolactone form towards the spirolactone form as indicated by an increased D50 value of the 4′-isomer compared to the D50 value of a reference isomer, in particular a 5′- or 6′-isomer, and preferably an absolute D50 value of the 4′-isomer which is a numeric value of at least 13 or 14, more preferred at least 15, 20, or 25, wherein said D50 value represents the dielectric constant at which the absorbance of a dye sample in a mixture of 1,4-dioxane and water is halved.
The D50 values indicated herein are determined by measuring the respective absorbance of a sample (dye isomer or derivative or conjugate thereof), in varied water-1,4-dioxane mixtures with known dielectric constants according to an established method (e.g. Åkerlöf G, Short AO. J Am Chem Soc 1936, 58(7): 1241-1243). D50 is commonly used as numeric value for the evaluation of the equilibrium changes and represents a dielectric constant at which absorbance is halved (Butkevich A. N.; Mitronova G. Y.; Sidenstein. S. C.; Klocke J. L.; Kamin D.; Meineke D. N. H.; D'Este E.; Kraemer P. T.; Danzl J. G.; Belov V. N.; Hell S. W., Angew. Chem. Int. Ed. 2016, 55, 3290-3294).
The D50 values were calculated using the following equation:
where A0—absorbance at λmax at εr=0, λmax—the highest reached absorbance at λmax, d—dielectric constant of 1,4-dioxane-water mixture at a given point, Hill—Hill slope coefficient determining the steepness of a dose-response curve, D50—corresponds to d value that provokes half of the absorbance amplitude (λmax−A0).
The above indicated absolute D50 value of 15 as obtained by said method corresponds to a mixture of 75% 1,4-dioxane and 25% water.
Preferably R1 to R13 are each independently selected from H, alkyl, in particular C1-C20 alkyl, C1-C12 alkyl, or C1-C6 alkyl.
X is preferably selected from O, CR14R15, SiR14R15, GeR14R15 where R14 and R15 are alkyl, in particular C1-C20 alkyl, C1-C12 alkyl, or C1-C6 alkyl, or aryl, in particular phenyl or naphthyl.
Said group Z which induces a neighboring group effect via steric, ionic or bonding interactions according to the invention is neither Cl, NH2 or NO2 and is preferably selected from the group consisting of C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, CON(aryl)2, C(O)O(alkyl) and C(O)O(aryl), with alkyl in particular C1-C20 alkyl, C1-C12 alkyl, or C1-C6 alkyl.
In some specific embodiments, the fluorescent dyes according to the present invention have one of the following structural formulae B-E
wherein the cyclic structure formed by R7 and R8 and/or by R9 and R10 taken together represents a non-aromatic heterocycle, in particular selected from azetidine, pyrrolidine, piperidine, morpholine, azepane or azecane;
wherein the cyclic structure formed by R2 and R8 and/or by R5 and R10 taken together represents an aromatic or non-aromatic 5 or 6 atom membered heterocyclic structure, in particular selected from azetidine, pyrrolidine, piperidine, azepane or azecane;
wherein the cyclic structure formed by R1 and R2 and/or by R5 and R6 taken together represents an aromatic or non-aromatic 5 or 6 atom membered heterocyclic structure, in particular selected from azetidine, pyrrolidine, piperidine, azepane or azecane;
wherein the cyclic structure formed by R3 and R7 and/or by R4 and R9 taken together represents an aromatic or non-aromatic 5 or 6 atom membered heterocyclic structure, in particular selected from azetidine, pyrrolidine, piperidine, azepane or azecane.
In other specific embodiments, the fluorescent dyes according to the present invention have one of the following structural formulae
wherein
R1, R2, R3, R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 are independently selected from H, halogen, D, CN, OH, O(alkyl), O(aryl), SH, S(alkyl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), N3, NH2, NH(alkyl), N(alkyl)2, NH(aryl), NH(aryl)2, NO2, CHO, C(O)(alkyl), C(O)(aryl), COOH, COO(alkyl) COO(aryl), C(O)NH(alkyl), C(O)NH(aryl), C(O)N(alkyl)2, C(O)N(aryl)2, P(O)OH(alkyl), P(O)OH(aryl), P(O)(—O-alkyl)2, P(O)(—O-aryl)2, PO3H2, SO3H, alkyl, bulky alkyl, substituted alkyl, alkenyl, substituted alkenyl, substituted bulky alkenyl;
R2 and R8 or R7 and R8 taken together may form a cyclic structure;
R5 and R9 or R9 and R10 taken together may form a cyclic structure;
R3 and R7 or R4 and R9 taken together may form a cyclic structure;
R1 and R2 or R5 and R6 taken together may form a cyclic structure;
Z is selected from O(alkyl), O(aryl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), S(O)2(—O-alkyl), S(O)2(—O-aryl), S(O)2NH(alkyl), S(O)2NH(aryl), S(O)2N(alkyl)2, S(O)2N(aryl)2, S(O)2N(alkyl)(aryl), C(O)O(alkyl), C(O)O(aryl), C(O)(alkyl), C(O)(aryl), P(O)OH(—NH-alkyl), P(O)OH(—O-alkyl), P(O)OH(—NH-aryl), P(O)OH(—O-aryl), P(O)(—O-alkyl)2, P(O)(—NH-alkyl)2, P(O)OH(—N(alkyl)2), P(O)OH(—N(aryl)2), P(O)(—N(aryl)2)2, P(O)(—N(alkyl)2)(—N(alkyl)2), P(O)(—O-aryl)2, P(O)(—NH-aryl)2, P(O)(—O-alkyl)(-O-aryl), P(O)(—NH-alkyl)(-O-aryl), P(O)(—O-alkyl)(-NH-aryl), P(O)(—NH-alkyl)(-NH-aryl), C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, in particular C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, CON(aryl)2, C(O)O(alkyl) and C(O)O(aryl), or any group which induces a neighboring group effect via steric, ionic or bonding interactions as defined above.
More specifically, the fluorescent dyes have one of the following structural formulae:
wherein
Z is selected from O(alkyl), O(aryl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), S(O)2(—O-alkyl), S(O)2(—O-aryl), S(O)2NH(alkyl), S(O)2NH(aryl), S(O)2N(alkyl)2, S(O)2N(aryl)2, S(O)2N(alkyl)(aryl), C(O)O(alkyl), C(O)O(aryl), C(O)(alkyl), C(O)(aryl), P(O)OH(—NH-alkyl), P(O)OH(—O-alkyl), P(O)OH(—NH-aryl), P(O)OH(—O-aryl), P(O)(—O-alkyl)2, P(O)(—NH-alkyl)2, P(O)OH(—N(alkyl)2), P(O)OH(—N(aryl)2), P(O)(—N(aryl)2)2, P(O)(—N(alkyl)2)(—N(alkyl)2), P(O)(—O-aryl)2, P(O)(—NH-aryl)2, P(O)(—O-alkyl)(-O-aryl), P(O)(—NH-alkyl)(-O-aryl), P(O)(—O-alkyl)(-NH-aryl), P(O)(—NH-alkyl)(-NH-aryl), C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, in particular C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, CON(aryl)2, C(O)O(alkyl) and C(O)O(aryl), or any group which induces a neighboring group effect via steric, ionic or bonding interactions as defined above.
The terms “rhodamine 4′-isomer” or “4′-isomer”, are used herein as an abbreviated designation of the claimed rhodamine compounds which are, i.a., characterized by the presence of a substituent group Z at position 4′ which is located next to the benzene ring position carrying the COOH group which is involved in spirolactone formation and, thus, immediately adjacent to said COOH group.
The following scheme illustrates the numbering of positions as used in the present application.
The term “substituted” as used herein, generally refers to the presence of one or more substituents, in particular substituents selected from the group comprising straight or branched alkyl, in particular C1-C4 alkyl, e.g. methyl, ethyl, propyl, butyl; isoalkyl, e.g. isopropyl, isobutyl (2-methylpropyl); secondary alkyl group, e.g. sec-butyl (but-2-yl); tert-alkyl group, e.g. tert-butyl (2-methylpropyl). Additionally, the term “substituted” may refer here to alkyl groups having at least one deuterium-, fluoro-, chloro- or bromo substituent instead of hydrogen atoms, or methoxy, ethoxy, 2-(alkyloxy)ethyloxy groups (alk-OCH2CH2O), and, in a more general case, oligo(ethylenglycol) residues of the art alk(OCH2CH2)nOCH2CH2—, where alk=CH3, C2H5, C3H7, C4H10, and n=1-12.
The term “alkyl” refers to any alkyl group selected from the group comprising straight or branched alkyl, more specifically C1-C20 alkyl, C1-C12 alkyl, or C1-C6 alkyl, e.g. methyl, ethyl, propyl, butyl; isoalkyl, e.g. isopropyl, isobutyl (2-methylpropyl); secondary alkyl group, e.g. sec-butyl (but-2-yl); tert-alkyl group, e.g. tert-butyl (2-methylpropyl) etc. The alkyl group may be a bulky alkyl group which comprises, e.g., a large and/or highly branched alkyl group such as a tert.-alkyl group.
The term “alkenyl” refers to any alkenyl group selected from the group comprising straight or branched alkenyl, more specifically C1-C20 alkenyl, C1-C12 alkenyl, or C1-C6 alkenyl, e.g. an alkenyl group corresponding to one of the exemplary alkyl groups mentioned above. The alkenyl group may be a bulky alkylenyl group which comprises, e.g., a large and/or highly branched alkenyl group such as a tert.-alkenyl group.
The terms “aryl” or “aromatic group”, as used herein, generally refer to an unsubstituted or substituted mono-, bi- or tricyclic carbocyclic ring system having one, two or three aromatic rings. Representative but not limiting examples are including but not limited to phenyl, naphthyl, anthryl, azulyl, tetrahydronaphthyl, indanyl and indenyl.
The terms “aromatic heterocyclic group” or “heteroaromatic group”, as used herein, generally refer to an unsubstituted or substituted cyclic aromatic radical (residue) having from 5 to 10 ring atoms of which at least one ring atom is selected from S, O and N; the radical being joined to the rest of the molecule via any of the ring atoms. Representative, but not limiting examples are furyl, thienyl, pyridinyl, pyrazinyl, pyrimidinyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, isooxazolyl, thiadiazolyl, oxadiazolyl, quinolinyl and isoquinolinyl.
The term “activated ester”, as used herein, generally refers to a carboxyl group substituted with a group which renders it more reactive with nucleophiles such as, but not limited to, free amino groups of peptides, polyaminoacids, polysaccharides, or analytes under such conditions that no interfering side reactions with other reactive groups of the nucleophile-carrying substance can usefully occur. Examples of such substituent groups that form activated esters directly or by introduction of the linker include N-succinimidyl, sulfo-N-succinimidyl, 1-benzotriazolyl, and the like.
The term “a positional isomer”, as used herein, generally refers to regioisomers of the discussed structures where a position of a functional group or other substituent on a parent structure changes, leading to different bonding patterns. The chemical composition and elemental analysis results of positional isomers are identical.
The term “linker”, as used herein, generally refers to a straight or branched alkyl chain which may be substituted by one or more functional groups and may include heteroatoms and/or aromatic groups and is connecting a targeting moiety (i.e. a reactive group or ligand) and fluorescent dye. More specifically, the linker may comprise or represent one or more of the following groups: Cn+1 alkyl linear or branched, e.g. methyl, ethyl, propyl, butyl; isoalkyl, e.g. isopropyl, isobutyl (2-methylpropyl); secondary alkyl group, e.g. sec-butyl (but-2-yl); tert-alkyl group, e.g. tert-butyl (2-methylpropyl), hydroxylated alkyl —(CH2O)n-linear or branched, fluorinated alkyl —(CHF)n+1— or —(CF2)n+1—, alkenyl —(CH2)k—(CH═CH)n+1—(CH2)m— with single or multiple double bonds, alkinyl —(CH2)k—(C≡C)n+1—(CH2)m— with single or multiple triple bonds, tris(hydroxymethyl)aminomethane, O(alkyl)2, O(aryl)2, S(alkyl)2, S(aryl)2, S(O)(alkyl)2, S(O)(aryl)2, S(O)2(alkyl)2, S(O)2(aryl)2, N(alkyl)2, NH(aryl)2, C(O)(alkyl)2, C(O)(aryl)2, P(O)(—O-alkyl)2, P(O)(—O-aryl)2, C(O)NH(alkyl)2. Here k, n and m=0-20.
The present invention further relates to rhodamine 4′-isomer derivatives or probes comprising a rhodamine 4′-isomer having one of the general formulae A-H as described above, wherein Z is selected from O(alkyl), O(aryl), S(aryl), S(O)(alkyl), S(O)(aryl), S(O)2(alkyl), S(O)2(aryl), S(O)2(—O-alkyl), S(O)2(—O-aryl), S(O)2NH(alkyl), S(O)2NH(aryl), S(O)2N(alkyl)2, S(O)2N(aryl)2, S(O)2N(alkyl)(aryl), C(O)O(alkyl), C(O)O(aryl), C(O)(alkyl), C(O)(aryl), P(O)OH(—NH-alkyl), P(O)OH(—O-alkyl), P(O)OH(—NH-aryl), P(O)OH(—O-aryl), P(O)(—O-alkyl)2, P(O)(—NH-alkyl)2, P(O)OH(—N(alkyl)2), P(O)OH(—N(aryl)2), P(O)(—N(aryl)2)2, P(O)(—N(alkyl)2)(—N(alkyl)2), P(O)(—O-aryl)2, P(O)(—NH-aryl)2, P(O)(—O-alkyl)(-O-aryl), P(O)(—NH-alkyl)(-O-aryl), P(O)(—O-alkyl)(-NH-aryl), P(O)(—NH-alkyl)(-NH-aryl), in particular C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, or any group which induces a neighboring group effect via steric, ionic or bonding interactions as defined above, which rhodamine 4′-isomer is coupled to at least one reactive group or ligand which is capable to interact with or bind to other molecules. Said reactive group or ligand may be coupled to the rhodamine 4′-isomer fluorophore either directly or via a linker as defined above.
More specifically, Z is selected from the group consisting of C(O)OH, C(O)NH(alkyl), C(O)NH(aryl), CON(alkyl)2, CON(aryl)2, C(O)O(alkyl) and C(O)O(aryl).
Typically, the linker comprises or consists of a straight or branched alkyl chain with 1-21 C atoms which may be substituted by one or more functional groups and may include heteroatoms and/or aromatic groups, and preferably represents a moiety selected from the following group:
with n=an integer of 1-20.
More specifically, said reactive group may be selected from the group comprising an activated ester, an amine, a thiol, an azide, an ethyne, a maleimide, a tetrazine, N-hydroxysuccinimide or an alcohol group, or wherein the ligand is a ligand which binds specifically to a protein, peptide, nucleotide or nucleic acid, carbohydrate, iodoacetamide or which is capable to effect or participate in chelation of NH4+ or metal ions, in particular Li+, Na+, K+, Cs+, Rb+, Cu+, Tl+, Hg+, Ag+, Au+, Ca2+, Ba2+, Sr2+, Be2+, Zn2+, Ni2+, Cu2+, Mg2+, Co2+, Fe2+, Mn2+, Pt2+, Cd2+, Hg2+, Sn2+, Pb2+, Au3+ Cr3+, Co3+, Fe3+, Al3+, Ga3+, In3+, Sc3+, Ti3+, Tl3+, V3+, Y3+, La3+, Pt4+, Pb4+, Ce4+, Ge4+, Th4+, Zr4+, U4+.
In a specific embodiment of the rhodamine 4′-isomer derivative according to the present invention, the reactive group or ligand is coupled directly or via a linker to group Z.
In this case, a linker may be also provided by Z, if said group Z as defined above already comprises a linking group, in particular as defined above.
In a preferred embodiment thereof, the reactive group or ligand is coupled by an amide bond formed between a carboxyl functional group or amine functional group provided by Z and an amine functional group or carboxyl functional group provided by the linker or ligand.
However, in other specific embodiments, the reactive group or ligand may be also coupled to other coupling sites on the same ring or other rings of the rhodamine structure, in particular at positions 6′ or 5′, preferably at position 6′.
In some other specific embodiments, the ligand of the rhodamine 4′-isomer derivative which is capable to interact with or bind to other molecules is benzylguanine and the protein is SNAP-tag, or the ligand is benzylcytosine and the proteine is CLIP-tag, or the ligand is a primary alkyl chloride and the protein is Halo-tag, or the ligand is trimethoprim and the protein is dihydrofolate reductase.
The rhodamine 4′-isomer derivative or probe of the invention has an increased D50 value compared to the D50 value of a reference isomer derivative, in particular a 5′- or 6′ isomer derivative, which is typically higher than that of the corresponding 5′- or 6′-isomer derivative by a difference of at least 10.
Preferably, the absolute D50 value of the 4′-isomer derivative or probe is a numeric value of at least 15, preferably at least 20 or 25, and generally is in the range from 15 to 85, typically in the range from 20 to 75 or from 25 to 70.
More specifically, if X═O in the above general formulae A-H, preferably the absolute D50 value of the 4′-isomer derivative or probe is in the range from 20 to 30 or 20 to 35, if X═CR14R15, preferably the absolute D50 value of the 4′-isomer derivative or probe is in the range from 55 to 70, if X═SiR14R15 or GeR14R15, preferably the absolute D50 value of the 4′-isomer derivative or probe is in the range from 70 to 80 or 70 to 85.
A further aspect of the present invention relates to conjugates comprising a rhodamine 4′-isomer or rhodamine 4′-isomer derivative as described above which is coupled to or associated with a molecule selected from the group comprising a peptide, protein, in particular an enzyme or antibody, tubulin or actin, a nucleotide, nucleic acid sequence, including DNA and RNA, a lipid, carbohydrate, an organic or inorganic polyphosphate, a drug, in particular a pharmaceutical drug, or a metabolite, in particular a metabolite of said drug, NH4+, a metal ion, in particular Li+, Na+, K+, Cs+, Rb+, Cu+, Tl+, Hg+, Ag+, Au+, Ca2+, Ba2+, Sr2+, Be2+, Zn2+, Ni2+, Cu2+, Mg2+, Co2+, Fe2+, Mn2+, Pt2+, Cd2+, Hg2+, Sn2+, Pb2+, Au3+ Cr3+, Co3+, Fe3+, Al3+, Ga3+, In3+, Sc3+, Ti3+, Tl3+, V3+, Y3+, La3+, Pt4+, Pb4+, Ce4+, Ge4+, Th4+, Zr4+, U4+, or complex thereof.
The conjugation may comprise formation of at least one covalent chemical bond or at least one molecular complex with a chemical entity or substance, such as amine, carboxylic acid, aldehyde, alcohol, aromatic compound, heterocycle, dye, amino acid, amino acid residue coupled to any chemical entity or molecule, in particular as listed above.
More specifically, the conjugation may comprise formation of at least one covalent chemical bond or at least one molecular complex with a chemical compound by a reaction or association with a functional group present in this compound such as amine, carboxylic acid, aldehyde, alcohol, aromatic compound, heterocycle, dye, amino acid, amino acid residue coupled to any chemical compound of interest, in particular as listed above.
The present invention provides a new class of rhodamine positional isomers, i.e. rhodamine 4′-isomers as defined above, and derivatives thereof that (i); possess high photostability (ii); have high extinction coefficients and high quantum yields (iii); can be derivatized with different molecules (iv); show little or no background binding to cellular biomolecules (v) are extremely membrane-permeable due to a neighboring group effect illustrated in the general structure of the new 4′-isomer dyes shown below
As mentioned, the essential feature of these novel fluorescent dyes is the presence of neighbouring groups Z in the benzene ring of the fluorophore. This provokes a specific effect, herein denoted as neighbouring group effect (NGE), which is a superposition of inductive, hydrogen bonding and steric effects (with the latter effects typically more relevant and a merely inductive effect typically will not be sufficient). For example, a significant NGE effect will be typically induced by groups Z which are bulky (steric interactions) and/or which are able to form hydrogen bonding with the carboxyl group responsible for spirolactonization. The exemplary groups Z listed above exhibit one or more of these properties.
This neighbouring group effect is only present in the 4′-isomers of rhodamines and influences the carboxyl group present in the benzene ring, changes spirolactonization rate and shifts equilibrium between spirolactone and zwitterion (see
Generally, the NGE of 4′-isomer derivatives is higher than that of corresponding 4′-isomers as such because of the steric and electronic effects induced by substitutents.
Surprisingly, this neigbouring group effect results in dramatically increased cell membrane permeability while keeping all photophysical properties of the compounds almost unchanged.
The general strategy for the synthesis of the novel rhodamine 4′ isomers is outlined above. More details with respect to the synthesis of exemplary compounds are provided in Synthesis Example A below.
Generally, a fluorescent probe is comprised of the fluorophore molecule connected to the ligand, which interacts with the target of interest. The fluorescent probe absorbs light of a specific wavelength and emits light of a different, typically longer, wavelength and it acts as a marker of target of interest for analysis with fluorescent microscopy or spectroscopy. Fluorophore defines the operational wavelengths and ligand defines the target of interest. Fluorophore and the ligand is connected by covalent bond directly or through the linker. Optimal length and hydrophilicity of the linker is usually specific for each target of interest.
The general strategy for the synthesis of rhodamine 4′ isomer based fluorescent probes is outlined above. More details with respect to the synthesis of exemplary probes/conjugates are provided in Synthesis Example B below.
In view of the advantageous properties of the novel fluorescent dyes according to the invention as outlined above, a further aspect of the present invention relates to their use as fluorescent dyes, in particular cell permeable fluorescent dyes, in a variety of applications.
Said fluorescent dyes may be a rhodamine 4′-isomer described above as such or a rhodamine 4′-isomer derivative comprising such a rhodamine 4′-isomer coupled to at least one reactive group or ligand which is capable to interact with or bind to other molecules. Said reactive group or ligand may be coupled to the rhodamine 4′-isomer fluorophore either directly or via a linker as defined above.
Said reactive group may be selected from the group comprising an activated ester, an amine, a thiol, an azide, an ethyne, a maleimide, a tetrazine, N-hydroxysuccinimide or an alcohol group, or wherein the ligand is a ligand which binds specifically to a protein, peptide, nucleotide or nucleic acid, carbohydrate, iodoacetamide or which is capable to effect or participate in chelation of NH4+ or metal ions, in particular Li+, Na+, K+, Cs+, Rb+, Cu+, Tl+, Hg+, Ag+, Au+, Ca2+, Ba2+, Sr2+, Be2+, Zn2+, Ni2+, Cu2+, Mg2+, Co2+, Fe2+, Mn2+, Pt2+, Cd2+, Hg2+, Sn2+, Pb2+, Au3+ Cr3+, Co3+, Fe3+, Al3+, Ga3+, In3+, Sc3+, Ti3+, Tl3+, V3+, Y3+, La3+, Pt4+, Pb4+, Ce4+, Ge4+, Th4+, Zr4+, U4+.
In the following, a number of suitable or preferred ligands for various main targets are listed:
In some specific applications, the ligand of the rhodamine 4′-isomer derivative which is capable to interact with or bind to other molecules is benzylguanine and the protein is SNAP-tag, or the ligand is benzylcytosine and the proteine is CLIP-tag, or the ligand is a primary alkyl chloride and the protein is Halo-tag, or the ligand is trimethoprim and the protein is dihydrofolate reductase.
The fluorescent dyes may be used as such or as conjugates with various organic substances, e.g. an amino acid (naturally occurring or not), a peptide, protein, in particular an enzyme or antibody, tubulin or actin, a nucleotide, nucleic acid sequence, including DNA and RNA, a lipid, carbohydrate, an organic or inorganic polyphosphate, a (pharmaceutical) drug or its metabolite, a toxin, NH4+, a metal ion, in particular, Li+, Na+, K+, Cs+, Rb+, Cu+, Tl+, Hg+, Ag+, Au+, Ca2+, Ba2+, Sr2+, Be2+, Zn2+, Ni2+, Cu2+, Mg2+, Co2+, Fe2+, Mn2+, Pt2+, Cd2+, Hg2+, Sn2+, Pb2+, Au+, Cr3+, Co3+, Fe3+, Al3+, Ga3+, In3+, Sc3+, Ti3+, Tl3+, V3+, Y3+, La3+, Pt4+, Pb4+, Ce4+, Ge4+, Th4+, Zr4+, U4+, or complex thereof.
The conjugation may comprise formation of at least one covalent chemical bond or at least one molecular complex with a chemical entity or substance, such as amine, carboxylic acid, aldehyde, alcohol, aromatic compound, heterocycle, dye, amino acid, amino acid residue coupled to any chemical entity or molecule, in particular as listed above.
More specifically, the conjugation may comprise formation of at least one covalent chemical bond or at least one molecular complex with a chemical compound by a reaction or association with a functional group present in this compound such as amine, carboxylic acid, aldehyde, alcohol, aromatic compound, heterocycle, dye, amino acid, amino acid residue coupled to any chemical compound of interest, in particular as listed above.
In a preferred embodiment of the present invention, the fluorescent dyes as described above or their conjugates are used as cell permeable substances penetrating through membranes of living and fixed cells in vivo or in vitro.
In one specific embodiment of the present invention, the fluorescent dyes as described above or their conjugates are used as such or after photoactivation for tracking and monitoring dynamic processes in a sample or in an object.
In a more specific embodiment, a rhodamine 4′-isomer derivative coupled to tubulin, DNA, RNA, lipid, or actin is used for cell cycle monitoring in living cells or living tissues.
In another specific embodiment, rhodamine 4′-isomers derivatives having a high singlet oxygen (1O2) quantum yield, preferably at least 5%, are used for chromophore-assisted light inactivation (CALI) and photodynamic therapy (PDT) in living cells and in living organisms such as humans, mammals, birds, fish, hemichordates, molluscs, tunicates, cnidarians, cephalochordates, flatworms, nematodes, annelids, tardigrades, reptiles, arthropods, echinoderms, chaetognathas, rotifers, frogs, plants, sponges or fungi.
The fluorescent dyes or their conjugates may be used as labels in microscopic, spectroscopic and other imaging techniques, in particular fluorescence microscopy and spectroscopy, in microfluidic devices, capillary electrophoresis, fluorescence activated cell sorting, DNA sequencing, sequence-specific genome labeling, analyte tracking techniques in vitro or in vivo.
Said imaging techniques comprise, e.g., stimulated emission depletion microscopy [STED], single molecule spectroscopy, single molecule switching (SMS) “nanoscopy” (diffraction unlimited optical resolution by using switching of the fluorescence of the single molecules, such as single molecule localization microscopy [SMLM], structured illumination microscopy (SIM), light-sheet microscopy, photoactivation localization microscopy [PALM, PALMIRA, fPALM], stochastic optical reconstruction microscopy [STORM]), fluorescence correlation spectroscopy [FCS] or fluorescence anisotropy spectroscopy, fluorescence recovery after photobleaching [FRAP], fluorescence lifetime imaging [FLIM], ground state depletion with individual molecular return [GSDIM], and fluorescence resonant energy transfer [FRET], correlative fluorescence-electron microscopy, correlative fluorescence—cryo-electron microscopy, microscale thermophoresis, fluorescence in situ hybridization (FISH), nuclear magnetic resonance spectroscopy.
Said analyte tracking techniques using a rhodamine 4′-isomer derivative of the present invention may be performed in vitro or in vivo and may be applied for tracking or monitoring a wide variety of analytes, in particular components of biological cells, such as proteins, lipids, carbohydrates, nucleic acids, sterols, nucleosides, nucleotides, polyphosphates, amino acids, polyamino acids, terpenes, lignines, chitin, chitosan, peptidoglycans, teichoic acids, lipoteichoic acid and vitamins.
In some exemplary and typical embodiments, a method or technique for tracking analytes using a rhodamine 4′-isomer derivative of the present invention comprises at least one of the following sequences of steps:
The following Examples are provided to illustrate the present invention in more detail, however, without limiting the invention to the specific conditions and parameters thereof.
All chemical reagents and solvents for synthesis were purchased from commercial suppliers (Sigma-Aldrich, Fluka, Acros) and were used without further purification. The composition of mixed solvents is given by the volume ratio (v/v).
NMR spectra were recorded at 25° C. with an Agilent 400-MR spectrometer at 400.06 MHz (1H) and 100.60 MHz (13C), Bruker Avance III HD 500 spectrometer (av500) at 500.25 MHz (1H) and 125.80 MHz (13C), Varian Mercury Plus 300 spectrometer at 300.14 MHz (1H), Varian INOVA 600 (1600) spectrometer at 599.74 MHz (1H) and are reported in ppm. All 1H and 11C spectra are referenced to tetramethylsilane (5=0 ppm) using the residual signals of the solvents according to the values reported in literature (Gottlieb H. E.; Kotlyar V.; Nudelman A., J Org Chem 1997, 62, 7512-7515). Multiplicities of signals are described as follows: s=singlet, d=doublet, t=triplet, q=quartet, p=pentet, m=multiplet or overlap of non-equivalent resonances; br=broad signal. Coupling constants (J) are given in Hz.
ESI-MS were recorded on a Varian 500-MS spectrometer (Agilent). ESI-HRMS were recorded on a MICROTOF spectrometer (Bruker) equipped with ESI ion source (Apollo) and direct injector with LC autosampler Agilent RR 1200. Liquid chromatography: Analytical LC-MS analysis was performed on an Agilent 1260 Infinity II LC/MS system equipped with an autosampler, diode array detector WR, fluorescence detector Spectra and Infinity Lab LC/MSD 6100 series quadruple with API electrospray. Analysis was done by using an Agilent Zorbax SB-C18 RRHT, 2.1×50 mm, 1.8 μm threaded column and SUPELCO Titan C18, 2.1×75 mm, 1.9 μm column with A: 25 mM HCOONH4 (pH=3.6) aqueous buffer and B: MeOH
Preparative HPLC was performed on an Interchim puriFlash 4250 2× preparative HPLC/Flash hybrid system (Article No. 1I5140, Interchim) with a 2 mL/5 mL injection loop, a 200-600 nm UV-Vis detector and an integrated ELSD detector (Article No. 1A3640, Interchim). Preparative column: Eurospher II 100-5 C18 5 μm, 250×20.0 mm (Article No.: 25PE181E2J, Knauer), typical flow rate: 25 mL/min, unless specified otherwise. Analytical TLC was performed on Merck Millipore ready-to-use plates with silica gel 60 (F254) (Cat. No. 1.05554.0001). Flash chromatography was performed on Biotage Isolera flash purification system using the type of cartridge and solvent gradient indicated.
Human primary dermal fibroblasts and HeLa cells were cultured in high-glucose DMEM (Life Technologies, Cat. No. 31053-028) supplemented with GlutaMAX-1 (Life Technologies, Cat. No. 35050-038) and 10% fetal bovine serum (FBS, Life Technologies, Cat. No. 10270-106) in a humidified 5% CO2 incubator at 37° C. The cells were split every 3-4 days or at confluence. Cells were seeded in glass bottom 12-well plates (MatTek Corporation, Cat. No. P12G-1.0-14-F).
Cells were stained with the fluorescent probes in DMEM (Thermo Fisher Scientific, Cat. No. 31053-028) supplemented with 10% FBS (Thermo Fisher Scientific, Cat. No. 10082139) at 37° C. and 5% CO2. If needed, the cells were washed 2 times with HBSS (Hanks' balanced salt solution, Lonza, Cat. No. BE10-527F) and imaged in DMEM with 10% FBS. No-wash experiments were performed in DMEM with 10% FBS after probe addition and incubation for the indicated period of time. For Airyscan experiments, cells were seeded in a 10-well plate (Greiner bio-one culture slides, PS, 75/25 mm. (Art. Nr.:543079)) 1 day before staining. The probes were applied to the cells in DMEM medium and after 1 h or overnight incubation cells were imaged without washing.
STED Microscope with 775 nm Depletion Laser
Comparative confocal and STED images were acquired on Abberior STED 775 QUAD scanning microscope (Abberior Instruments GmbH, Germany) equipped with 561 nm and 640 nm 40 MHz pulsed excitation lasers, a pulsed 775 nm 40 MHz STED laser, and an UPlanSApo 100×/1.40 Oil objective. The following detection windows were used: TMR/580CP channel 615/20 nm and 610CP/SiR channel 685/70 nm. In this setup, voxel size was 15-30 nm in xy plane and 150 nm in z-axis for STED images.
Measurements of the absorbance changes in 1,4-dioxane-water mixtures were performed by pipetting 2 μL stock solutions of dyes or probes in DMSO into a 96 glass bottom well plate (11 wells per sample) made from propylene (Corning 3364). To the wells going from right to left 300 μL of 1,4-dioxane-water mixtures containing 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% or 0% 1,4-dioxane was added (if needed, mixtures with 0.3% SDS are used, with an exception in 100% dioxane due to solubility issues). After incubation for 1 hour at room temperature, absorption of solutions in each well was recorded from 320 nm to 850 nm with wavelength step size of 1 nm on a multiwell plate reader Spark® 20M (Tecan). The background absorption of the glass bottom plate was measured in wells containing only 1,4-dioxane-water mixture with similar amount of DMSO and subtracted from the spectra of the samples. Plots of λmax versus dielectric constant (D) of 1,4-dioxane-water mixtures (G. Åkerlöf, A. O. Short, The Dielectric Constant of Dioxane-Water Mixtures between 0 and 80°, J. Am. Chem. Soc. 1936 58(7) 1241-1243). D50 value was obtained by fitting to dose-response equation EC50 as implemented in GraphPad 6.0 software:
where A0—absorbance at λmax at εr=0, λmax—the highest reached absorbance at λmax. d—dielectric constant of 1,4-dioxane-water mixture at a given point, Hill—Hill slope coefficient determining the steepness of a dose-response curve, D50—corresponds to d value that provokes half of the absorbance amplitude (λmax−A0).
HeLa cells were grown in 6-well plates (˜250.000 cells per well) for 24 h in the presence of the fluorescent probe in variable concentrations. The probes were dissolved in DMSO at 500-2000-fold stock concentration and added to the media of cultured cells at 500-2000-fold dilution accordingly. In parallel, the appropriate DMSO control samples were prepared by adding corresponding amount of DMSO volume to the separate well. Cells were processed according to the NucleoCounter® NC-3000™ two-step cell cycle analysis protocol for cells attached to T-flasks, cell culture plates or micro-carriers. In particular, the 250 μl lysis solution (Solution 10, Chemometec Cat. No. 910-3010) supplemented with 10 μg/ml DAPI (Solution 12, Chemometec Cat. No. 910-3012) was used per well, incubated at 37° C. for 5 min. Then 250 μl of stabilization solution (Solution 11, Chemometec Cat. No. 910-3011) was added. Cells were counted on a NucleoCounter® NC-3000™ in NC-Slide A2™ slides (Chemometec, Cat. No. 942-0001) loaded with ˜30 μl of each of the cell suspensions into the chambers of the slide. Each time, ˜10.000 cells in total were measured, and the obtained cell cycle histograms were analysed with ChemoMetec NucleoView NC-3000 software, version 2.1.25.8. All experiments were repeated three times and the results are presented as means with standard deviations. The EC50 values were determined by plotting the percentage of cells in subG1 phase and fitting the curve in GraphPad Prism 6 to the following function:
where Ymin—cells population percentage in subG1 phase then no probe was added, Ymax—highest reachable percentage of cells in subG1 phase and shared value for all data sets equal to 69%. X—cells population percentage in subG1 phase then added probe is at X concentration, Hill—Hill slope coefficient determining the steepness of a dose-response curve, EC50—the concentration of probe that provoking halfway of subG1 cells in a population between the baseline (Ymin) and maximum response (Ymax).
All acquired or reconstructed images were processed and visualized using Fiji (J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J. Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, A. Cardona, Fiji: an open-source platform for biological-image analysis, Nat Methods 2012 9(7) 676-82). Line profiles were measured using the “straight line” tool with the line width set to 3 pixels.
For the signal measurements, image files were converted to TIF file using Fiji and analyzed with CellProfiler 3.1.8 (A. E. Carpenter, T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, D. M. Sabatini, CellProfiler: image analysis software for identifying and quantifying cell phenotypes, Genome Biol 2006 7(10) R100), where the pipeline identified the nuclear region and measured the mean signal in this region. Background signal was measured in the region which is 3 pixels (450 nm) away from the nuclear border and 7 pixels (1050 nm) wide. The background subtracted signal was processed with GraphPad Prism 6.
Actin/tubulin cytosolic signal was estimated using CellProfiler v.3.1.8. Briefly, probe channel was smoothed using median filter, nuclei were identified in DAPI channel and were used as seeds to find cell outlines in a smoothed actin channel. Background was defined as a lower quartile of pixel intensity in the area not covered by cells in the original probe channel. The background was subtracted from the original probe channel, and actin/tubulin staining was measured as mean pixel intensity per object in background-corrected probe channel. Statistical analysis performed by GraphPad Prism 6.
For assessing living cell staining, U2OS cells expressing vimentin-Halo-tag were grown on 35 mm diameter plastic plates in DMEM with 10% FBS. 100 nM x-610CP-Halo substrates were added and the cells were incubated at 37° C. for 1 h. Then, the cells were washed twice with growth medium and twice with HBSS. 200 μl of CelLytic M solution (Sigma), supplemented with complete EDTA-free protease inhibitor cocktail (Sigma) was added, and the cells were allowed to lyse for 15 min. at room temperature with gentle agitation. The solution was collected and centrifuged for 15 min. at 13000 rpm (4° C.). 180 μl of supernatant was mixed with 60 μl 4×SDS-PAGE sample buffer and boiled for 5 min.
To assess reactivity in the cell extracts, the cells were lysed as described above, and the lysates were incubated with 100 nM HaloTag substrates for 30 min. at 37° C. before boiling. The samples were fractionated in 4-15% gradient.
3-Bromophtalic acid (1.0 g, 4.08 mmol) was suspended in DCM (15 mL) in a sealable pressure glass tube. The mixture was cooled in a NaCl/ice bath and ˜10 mL of isobutylene gas was condensed in the mixture. Catalytic amount of concentrated sulphuric acid (0.1 mL, 1.87 mmol) was added to the stirred and cooled reaction mixture and the pressure tube was tightly sealed. Reaction mixture was stirred at room temperature for 48 h, during this time the suspension became a clear solution. Then reaction mixture was cooled in ice bath and the tube was carefully opened with release of pressure. The resulting solution was poured to saturated NaHCO3 solution (50 mL), extracted with DCM (2×30 mL). The organic extracts were combined and washed with water and brine, dried over Na2SO4. The product was isolated by flash column chromatography (Teledyne Isco RediSep Rf 40 g, isocratic hold 5% of EtOAc in Hexane), fractions containing the product were evaporated to give 1.02 g (70%) of white solid.
1H NMR (400 MHz, cdcl3) δ 7.79 (dd, J=7.8, 1.1 Hz, 1H), 7.67 (dd, J=8.0, 1.1 Hz, 1H), 7.23 (t, J=7.9 Hz, 1H), 1.61 (s, 9H), 1.56 (s, 9H).
13C NMR (101 MHz, cdcl3) δ 165.9, 163.6, 137.9, 136.1, 131.6, 129.5, 128.5, 120.3, 83.0, 82.1, 28.1, 28.0.
ESI-MS, positive mode: m/z=357.1, 359.1 [M+H]+.
HRMS (ESI) calcd for C16H22N2BrO4 [M+H]+ 357.0696, found 357.0698.
In a 25 mL round-bottom flask, a degassed solution of 1 (740 mg, 2.07 mmol, 2 eq.) in anhydrous THF (6 mL) and pentane (4 mL) was cooled to ˜−116° C. (diethyl ether-liquid N2). n-Butyllithium (1.3 mL of 1.6 M solution in hexanes, 2.07 mmol, 2 eq.) was carefully introduced through a needle. Clear solution quickly turned orange and then deep brown; it was stirred at ˜−116° C. for 10 min, and the solution of ketone 2 (475 mg, 1.04 mmol, 1 eq.) (Martinez-Peragon, A.; Miguel, D.; Jurado, R.; Justicia, J.; Alvarez-Pez, J. M.; Cuerva, J. M.; Crovetto, L., Chemistry, 2014, 20 (2), 447-55) in THF (3 mL) was injected along the wall of the flask. Stirred at ˜−116° C. for 10 minutes and then flask was taken out of the cooling bath and left to warm to rt and stirred for further 30 min. The reaction mixture was quenched with water (10 mL), adjusted to pH ˜5 with acetic acid, extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Büchi Reveleris HP silica 40 g; gradient 0% to 20% ethyl acetate-hexane.) In some cases additional purification was needed (Teledyne Isco RediSep Rf 40 g, gradient 20% to 100% of DCM-Hexane). Product yield 433 mg (63%) of off-white solid.
1H NMR (400 MHz, d6-acetone) δ 7.86 (t, J=7.6 Hz, 1H), 7.82 (dd, J=7.6, 1.4 Hz, 1H), 7.40 (dd, J=7.6, 1.4 Hz, 1H), 6.81 (d, J=2.4 Hz, 2H), 6.76 (d, J=8.6 Hz, 2H), 6.70 (dd, J=8.6, 2.4 Hz, 2H), 1.66 (s, 9H), 1.00 (s, 18H), 0.27 (s, 12H).
13C NMR (101 MHz, d6-acetone) δ 165.9, 165.0, 157.6, 153.9, 152.1, 135.2, 133.1, 129.3, 129.1, 126.0, 123.2, 116.9, 112.4, 107.4, 82.4, 81.3, 27.2, 25.0, 17.9, −5.3.
ESI-MS, positive mode: m/z=661.3 [M+H]+.
HRMS (ESI) calcd for C37H49O7Si2 [M+H]+ 661.3011, found 661.3004.
In a 25 mL round-bottom flask, a degassed solution of 1 (740 mg, 2.07 mmol, 2 eq.) in anhydrous THF (6 mL) and pentane (4 mL) was cooled to ˜−116° C. (diethyl ether-liquid N2). n-Butyllithium (1.3 mL of 1.6 M solution in hexanes, 2.07 mmol, 2 eq.) was carefully introduced through a needle. Clear solution quickly turned orange and then deep brown; it was stirred at ˜−116° C. for 10 min, and the solution of ketone 3 (502 mg, 1.04 mmol, 1 eq.) (Grimm, J. B.; Sung, A. J.; Legant, W. R.; Hulamm, P.; Matlosz, S. M.; Betzig, E.; Lavis, L. D., ACS Chem Biol, 2013, 8(6), 1303-1310) in THF (3 mL) was injected along the wall of the flask. Stirred at ˜−116° C. for 10 minutes and then flask was taken out of the cooling bath and left to warm to rt and stirred for further 30 min. The reaction mixture was quenched with water (10 mL), adjusted to pH ˜5 with acetic acid, extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Büchi Reveleris HP silica 40 g; gradient 0% to 20% ethyl acetate-hexane.) In some cases additional purification was needed (Teledyne Isco RediSep Rf 40 g, gradient 20% to 100% of DCM-Hexane). Product yield 471 mg (66%) of off-white solid.
1H NMR (600 MHz, cdcl3) δ 7.72 (dd, J=7.6, 0.8 Hz, 1H), 7.58 (t, J=7.6 Hz, 1H), 7.07 (d, J=0.8 Hz, 1H), 7.05 (d, J=2.0 Hz, 2H), 6.66 (d, J=8.6 Hz, 2H), 6.61 (dd, J=8.6, 2.0 Hz, 2H), 1.79 (s, 3H), 1.71 (s, 3H), 1.70 (s, 9H), 0.98 (s, 18H), 0.21 (s, 12H).
13C NMR (126 MHz, cdcl3) δ 167.3, 165.3, 156.4, 156.1, 146.6, 134.1, 133.0, 129.2, 128.9, 125.7, 124.1, 123.2, 118.9, 117.4, 85.0, 83.3, 38.0, 34.9, 33.1, 28.1, 25.7, 18.3, −4.2,
ESI-MS, positive mode: m/z=687.4 [M+H]+.
HRMS (ESI) calcd for C40H55O6Si2 [M+H]+ 687.3532, found 687.3523.
2.07 mmol, 2 eq.) in anhydrous THF (6 mL) and pentane (4 mL) was cooled to ˜−116° C. (diethyl ether-liquid N2). n-Butyllithium (1.3 mL of 1.6 M solution in hexanes, 2.07 mmol, 2 eq.) was carefully introduced through a needle. Clear solution quickly turned orange and then deep brown; it was stirred at ˜−116° C. for 10 min, and the solution of ketone 4 (520 mg, 1.04 mmol, 1 eq.) (Butkevich, A. N.; Belov, V. N.; Kolmakov, K.; Sokolov, V. V.; Shojaei, H.; Sidenstein, S. C.; Kamin, D.; Matthias, J.; Vlijm, R.; Engelhardt, J.; Hell, S. W., Chemistry—A European Journal, 2017, 23(50), 12114-12119) in THF (3 mL) was injected along the wall of the flask. Stirred at ˜−116° C. for 10 minutes and then flask was taken out of the cooling bath and left to warm to rt and stirred for further 30 min. The reaction mixture was quenched with water (10 mL), adjusted to pH ˜5 with acetic acid, extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Büchi Reveleris HP silica 40 g; gradient 0% to 20% ethyl acetate-hexane.) In some cases additional purification was needed (Teledyne Isco RediSep Rf 40 g, gradient 20% to 100% of DCM-Hexane). Product yield 446 mg (61%) of off-white solid.
1H NMR (400 MHz, cdcl3) δ 7.69 (dd, J=7.6, 1.0 Hz, 1H), 7.62 (t, J=7.6 Hz, 1H), 7.31 (dd, J=7.6, 1.0 Hz, 1H), 7.09 (d, J=2.7 Hz, 2H), 6.87 (dd, J=8.7, 0.4 Hz, 2H), 6.67 (dd, J=8.7, 2.7 Hz, 2H), 1.65 (s, 9H), 0.96 (s, 18H), 0.60 (s, 3H), 0.56 (s, 3H), 0.17 (s, 12H).
13C NMR (101 MHz, cdcl3) δ 167.5, 165.6, 155.2, 155.2, 137.2, 136.9, 134.0, 133.5, 128.9, 128.6, 126.3, 125.0, 123.1, 121.2, 89.1, 83.4, 28.1, 25.7, 18.3, −1.3, −2.9, −4.3, −4.
ESI-MS, positive mode: m/z=703.3 [M+H]+.
HRMS (ESI) calcd for C39H55O6Si3 [M+H]+ 703.3301, found 703.3304.
To a cooled (ice-water bath) solution of corresponding compound 5 (420 mg, 0.635 mmol, 1 eq.) in THF (15 mL) tetrabutylammonium fluoride trihydrate (800 mg, 2.54 mmol, 4 eq.) solution in THF (5 mL) was added. The resulting intensively coloured solution was stirred at 0° C. for 1 h. Sat. aq. NH4Cl (20 mL) was added followed by minimal amount of water necessary to dissolve the solids, the mixture was extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 2% to 30% ethyl acetate-CH2Cl2) and evaporated to obtain viscous oil which solidifies overtime. Product yield 214 mg (78%) of orange solid material.
1H NMR (400 MHz, d6-dmso) δ 10.15 (s, 2H), 7.87-7.76 (m, 2H), 7.39 (dd, J=7.3, 1.4 Hz, 1H), 6.69 (d, J=2.0 Hz, 2H), 6.63-6.47 (m, 4H), 1.60 (s, 9H).
13C NMR (101 MHz, d6-dmso) δ 166.1, 164.9, 159.5, 153.4, 151.9, 135.6, 132.2, 129.2, 128.9, 126.3, 122.6, 112.7, 109.3, 102.3, 82.6, 82.2, 27.7.
ESI-MS, positive mode: m/z=433.1 [M+H]+.
HRMS (ESI) calcd for C25H21O7[M+H]+ 433.1282, found 433.1279.
To a cooled (ice-water bath) solution of corresponding compound 6 (445 mg, 0.647 mmol, 1 eq.) in THF (15 mL) tetrabutylammonium fluoride trihydrate (817 mg, 2.59 mmol, 4 eq.) solution in THF (5 mL) was added. The resulting intensively coloured solution was stirred at 0° C. for 1 h. Sat. aq. NH4Cl (20 mL) was added followed by minimal amount of water necessary to dissolve the solids, the mixture was extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 2% to 30% ethyl acetate-CH2Cl2) and evaporated to obtain viscous oil which solidifies overtime. Product yield 264 mg (89%) of orange solid material.
1H NMR (300 MHz, cdcl3) δ 7.73 (dd, J=7.6, 1.0 Hz, 1H), 7.60 (t, J=7.6 Hz, 1H), 7.14 (s, 2H), 7.05 (dd, J=7.6, 1.0 Hz, 1H), 6.98 (d, J=2.4 Hz, 2H), 6.51 (dd, J=8.6, 2.4 Hz, 2H), 6.44 (d, J=8.6 Hz, 2H), 1.68 (s, 9H).
13C NMR (126 MHz, cdcl3) δ 169.0, 165.9, 156.7, 156.2, 147.3, 134.7, 132.7, 129.2, 129.1, 126.1, 123.2, 122.4, 114.8, 112.9, 87.0, 84.1, 38.1, 34.8, 32.3, 28.1.
ESI-MS, positive mode: m/z=459.2 [M+H]+.
HRMS (ESI) calcd for C28H27O6[M+H]+ 459.1802, found 459.1805.
To a cooled (ice-water bath) solution of corresponding compound 7 (420 mg, 0.575 mmol, 1 eq.) in THF (15 mL) tetrabutylammonium fluoride trihydrate (725 mg, 2.3 mmol, 4 eq.) solution in THF (5 mL) was added. The resulting intensively coloured solution was stirred at 0° C. for 1 h. Sat. aq. NH4Cl (20 mL) was added followed by minimal amount of water necessary to dissolve the solids, the mixture was extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 2% to 30% ethyl acetate-CH2Cl2) and evaporated to obtain viscous oil which solidifies overtime. Product yield 254 mg (93%) of orange solid material.
1H NMR (400 MHz, d6-dmso) δ 9.74 (s, 2H), 7.83 (t, J=7.6 Hz, 1H), 7.72 (dd, J=7.6, 0.9 Hz, 1H), 7.46 (dd, J=7.6, 0.9 Hz, 1H), 7.16-7.08 (m, 2H), 6.74-6.60 (m, 4H), 1.55 (s, 9H), 0.56 (s, 3H), 0.48 (s, 3H).
13C NMR (101 MHz, d6-dmso) δ 167.4, 165.4, 157.3, 154.6, 137.5, 135.2, 134.5, 133.5, 129.0, 128.6, 127.1, 122.3, 120.7, 117.3, 90.0, 83.0, 28.0, 0.4, −1.4.
ESI-MS, positive mode: m/z=475.2 [M+H]+.
HRMS (ESI) calcd for C27H27O6Si [M+H]+ 475.1571, found 475.1573.
Trifluoromethanesulfonic anhydride 1M solution in DCM (1.85 mL, 1.85 mmol, 4 eq.) was slowly added dropwise to a solution of corresponding compound 8 (200 mg, 0.463 mmol, 1 eq.) and pyridine (0.3 mL, 3.7 mmol; 8 eq.) in dry DCM (10 mL), cooled in ice-water bath. The flask was then removed from the cooling bath, and the mixture was stirred at rt for 1 h. Afterwards, the mixture was diluted with water (30 mL), extracted with CH2Cl2 (3×20 mL), the combined extracts were washed with water, brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 5% to 40% ethyl acetate-hexane).
Product yield 255 mg (79%) of white solid material.
1H NMR (400 MHz, cdcl3) δ 7.87 (d, J=7.4 Hz, 1H), 7.73 (t, J=7.4 Hz, 1H), 7.30 (d, J=2.2 Hz, 2H), 7.22 (d, J=7.4 Hz, 1H), 7.08-6.99 (m, 4H), 1.70 (s, 9H).
13C NMR (101 MHz, cdcl3) δ 165.4, 164.6, 153.3, 151.2, 150.2, 135.4, 133.7, 130.7, 130.0, 125.7, 122.4, 119.1, 118.6 (q, 1JC-F=319 Hz, —CF3), 117.7, 110.7, 83.9, 78.8, 28.0.
ESI-MS, positive mode: m/z=697.0 [M+H]+.
HRMS (ESI) calcd for C27H19F6O11S2[M+H]+ 697.0267, found 697.0249.
Trifluoromethanesulfonic anhydride 1M solution in DCM (2.14 mL, 2.14 mmol, 4 eq.) was slowly added dropwise to a solution of corresponding compound 9 (245 mg, 0.535 mmol, 1 eq.) and pyridine (0.35 mL, 4.3 mmol; 8 eq.) in dry DCM (10 mL), cooled in ice-water bath. The flask was then removed from the cooling bath, and the mixture was stirred at rt for 1 h. Afterwards, the mixture was diluted with water (30 mL), extracted with CH2Cl2 (3×20 mL), the combined extracts were washed with water, brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 5% to 40% ethyl acetate-hexane). Product yield 271 mg (70%) of white solid material.
1H NMR (400 MHz, cdcl3) δ 7.83 (dd, J=7.6, 0.9 Hz, 1H), 7.68 (t, J=7.6 Hz, 1H), 7.54 (d, J=2.5 Hz, 2H), 7.11 (dd, J=8.8, 2.5 Hz, 2H), 7.07 (dd, J=7.6, 0.9 Hz, 1H), 6.94 (d, J=8.8 Hz, 2H), 1.89 (s, 3H), 1.79 (s, 3H), 1.70 (s, 9H).
13C NMR (101 MHz, cdcl3) δ 166.4, 164.8, 154.8, 150.1, 146.9, 135.1, 133.8, 131.3, 130.3, 130.2, 125.6, 122.6, 120.3, 119.6, 118.9 (q, 1JC-F=319 Hz,—CF3), 83.8, 82.6, 38.8, 34.7, 33.0, 28.0.
ESI-MS, positive mode: m/z=745.1 [M+Na]+.
HRMS (ESI) calcd for C30H25O10S2F6[M+H]+ 723.0788, found 723.0800.
Trifluoromethanesulfonic anhydride 1M solution in DCM (1.94 mL, 1.94 mmol, 4 eq.) was slowly added dropwise to a solution of corresponding compound 10 (230 mg, 0.484 mmol, 1 eq.) and pyridine (0.31 mL, 3.87 mmol; 8 eq.) in dry DCM (10 mL), cooled in ice-water bath. The flask was then removed from the cooling bath, and the mixture was stirred at rt for 1 h. Afterwards, the mixture was diluted with water (30 mL), extracted with CH2Cl2 (3×20 mL), the combined extracts were washed with water, brine and dried over Na2SO4. The products were isolated by flash column chromatography (Teledyne Isco RediSep Rf 24 g; gradient 5% to 40% ethyl acetate-hexane). Product yield 271 mg (70%) of white solid material. Product yield 258 mg (72%) of white solid material.
1H NMR (400 MHz, cdcl3) δ 7.81 (dd, J=7.6, 1.1 Hz, 1H), 7.75 (t, J=7.6 Hz, 1H), 7.56 (dd, J=2.3, 0.8 Hz, 2H), 7.39 (dd, J=7.6, 1.1 Hz, 1H), 7.24-7.17 (m, 4H), 1.67 (s, 9H), 0.74 (s, 3H), 0.70 (s, 3H).
13C NMR (101 MHz, cdcl3) δ 166.2, 164.8, 152.8, 149.3, 144.0, 138.7, 134.8, 134.3, 130.0, 129.2, 126.3, 126.1, 122.8, 122.6, 118.7 (q, 1JC-F=319 Hz,—CF3), 87.3, 83.8, 28.0, −0.2, −1.7.
ESI-MS, positive mode: m/z=761.0 [M+Na]+.
HRMS (ESI) calcd for C29H25O10S2SiF6 [M+H]+ 739.0557, found 739.0562.
A mixture of Pd2(dba)3 (25.6 mg, 0.026 mmol, 0.1 eq.), Xantphos (44.8 mg, 0.077 mmol, 0.3 eq.), Cs2CO3 (251 mg, 0.77, 3 eq.) and corresponding triflate 11 (180 mg, 0.258 mmol, 1 eq) in dry 1,4-dioxane (2.5 mL) was degassed on a Schlenk line. Then 2M solution of dimethylamine in THF (323 μL, 0.645 mmol, 2.5 eq.) were introduced. Reaction mixture was stirred in a septa sealed tube at 100° C. under argon for 18 h. Upon cooling, the resulting brown mixture was diluted with water (30 mL), pH adjusted to 5-6 with AcOH and extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. The filtrate was evaporated and the product was isolated by flash chromatography Büchi Reveleris HP silica 24 g; gradient 2% to 30% MeOH-DCM. The fractions containing the product were evaporated, the residue was redissolved in acetonitrile-water (1:1), microfiltered through a 0.45 μm PTFE membrane filter and lyophilized to obtain 67 mg (53%) of pink solid. 1H NMR (400 MHz, cd3od) δ 8.00 (dd, J=7.8, 1.3 Hz, 1H), 7.57 (t, J=7.8 Hz, 1H), 7.39 (dd, J=7.8, 1.3 Hz, 1H), 7.31 (d, J=9.5 Hz, 2H), 7.00 (dd, J=9.5, 2.5 Hz, 2H), 6.85 (d, J=2.5 Hz, 2H), 3.26 (s, 12H), 1.61 (s, 9H).
13C NMR (101 MHz, cd3od) δ 173.7, 167.8, 159.1, 158.9, 158.8, 144.0, 133.6, 133.3, 131.9, 131.8, 131.6, 128.1, 115.3, 114.9, 97.3, 83.3, 40.9, 28.3.
ESI-MS, positive mode: m/z=487.2 [M+H]+.
HRMS (ESI) calcd for C29H31N2O5 [M+H]+ 487.2227, found 487.2232
A mixture of Pd2(dba)3 (30.2 mg, 0.033 mmol, 0.1 eq.), Xantphos (57.3 mg, 0.099 mmol, 0.3 eq.), Cs2CO3 (322.5 mg, 0.99 mmol, 3 eq.) and corresponding triflate 12 (240 mg, 0.33 mmol, 1 eq) in dry 1,4-dioxane (2.5 mL) was degassed on a Schlenk line. Then solution of tert-butyl N-methylcarbamate (108 mg, 0.825 mmol, 2.5 eq.) in 1,4-dioxane (0.5 mL) were introduced. Reaction mixture was stirred in a septa sealed tube at 100° C. under argon for 18 h. Upon cooling, the resulting brown mixture was diluted with water (30 mL), pH adjusted to 5-6 with AcOH extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. Product was isolated by flash column chromatography Büchi Reveleris HP silica 24 g; gradient 5% to 50% EtOAc-Hexane. The fractions containing the product were evaporated, the residue was redissolved in acetonitrile-water (1:1), microfiltered through a 0.45 μm PTFE membrane filter and lyophilized to obtain 173 mg (76%) of violet powder.
1H NMR (400 MHz, cdcl3) δ 7.75 (dd, J=7.6, 0.9 Hz, 1H), 7.59 (t, J=7.6 Hz, 1H), 7.52 (d, J=2.2 Hz, 2H), 7.06 (dd, J=7.6, 0.9 Hz, 1H), 7.01 (dd, J=8.6, 2.2 Hz, 2H), 6.76 (d, J=8.6 Hz, 2H), 3.27 (s, 6H), 1.85 (s, 3H), 1.76 (s, 3H), 1.70 (s, 9H), 1.46 (s, 18H).
13C NMR (101 MHz, cdcl3) δ 167.4, 165.5, 156.2, 154.6, 145.3, 144.7, 134.5, 133.4, 129.5, 128.4, 127.9, 126.0, 123.6, 123.6, 123.2, 84.4, 83.6, 80.8, 38.3, 37.3, 35.0, 33.3, 28.5, 28.2.
ESI-MS, positive mode: m/z=685.4 [M+H]+.
HRMS (ESI) calcd for C40H49N2O8 [M+H]+ 685.3483, found 685.3474.
A mixture of Pd2(dba)3 (33 mg, 0.036 mmol, 0.1 eq.), Xantphos (62.5 mg, 0.108 mmol, 0.3 eq.), Cs2CO3 (352 mg, 1.08 mmol, 3 eq.) and corresponding triflate 12 (250 mg, 0.36 mmol, 1 eq) in dry 1,4-dioxane (2.5 mL) was degassed on a Schlenk line. Then 2M solution of dimethylamine in THF (450 μL, 0.9 mmol, 2.5 eq.) were introduced. Reaction mixture was stirred in a septa sealed tube at 100° C. under argon for 18 h. Upon cooling, the resulting brown mixture was diluted with water (30 mL), pH adjusted to 5-6 with AcOH and extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4. Product was isolated by flash column chromatography Büchi Reveleris HP silica 24 g; gradient 20% to 80% EtOAc-Hexane. The fractions containing the product were evaporated, the residue was redissolved in 1,4-dioxane-water (1:1), microfiltered through a 0.45 μm PTFE membrane filter and lyophilized to obtain 131 mg (71%) of violet powder.
1H NMR (400 MHz, cdcl3) δ 7.69 (dd, J=7.6, 1.0 Hz, 1H), 7.55 (t, J=7.6 Hz, 1H), 7.09 (dd, J=7.6, 1.0 Hz, 1H), 6.88 (d, J=2.6 Hz, 2H), 6.65 (d, J=8.8 Hz, 2H), 6.53 (dd, J=8.8, 2.6 Hz, 2H), 2.99 (s, 12H), 1.87 (s, 3H), 1.78 (s, 3H), 1.70 (s, 9H).
13C NMR (101 MHz, cdcl3) δ 167.9, 166.0, 157.0, 150.8, 146.7, 134.0, 133.0, 129.1, 128.8, 126.1, 124.0, 119.5, 111.8, 109.2, 86.5, 83.3, 40.6, 38.6, 35.6, 33.1, 28.2.
ESI-MS, positive mode: m/z=513.6 [M+H]+.
HRMS (ESI) calcd for C32H37N2O4 [M+H]+ 513.2748, found 513.2748.
A mixture of Pd2(dba)3 (26 mg, 0.0284 mmol, 0.1 eq.), Xantphos (49.3 mg, 0.0852 mmol, 0.3 eq.), Cs2CO3 (278 mg, 0.85 mmol, 3 eq.) and corresponding triflate 13 (210 mg, 0.284 mmol, 1 eq) in dry 1,4-dioxane (2.5 mL) was degassed on a Schlenk line. Then 2M solution of dimethylamine in THF (355 μL, 0.71 mmol, 2.5 eq.) were introduced. Reaction mixture was stirred in a septa sealed tube at 100° C. under argon for 18 h. Upon cooling, the resulting brown mixture was diluted with water (30 mL), pH adjusted to 5-6 with AcOH and extracted with ethyl acetate (3×30 mL), the combined organic layers were washed with brine and dried over Na2SO4.
Product was isolated by flash column chromatography Büchi Reveleris HP silica 24 g; gradient 5% to 50% EtOAc-Hexane. The fractions containing the product were evaporated, the residue was redissolved in 1,4-dioxane-water (1:1), microfiltered through a 0.45 μm PTFE membrane filter and lyophilized to obtain 73 mg (48%) of light-blue powder.
1H NMR (400 MHz, cdcl3) δ 7.69 (dd, J=7.6, 1.0 Hz, 1H), 7.61 (t, J=7.6 Hz, 1H), 7.32 (dd, J=7.6, 1.0 Hz, 1H), 6.96 (d, J=2.9 Hz, 2H), 6.82 (d, J=8.9 Hz, 2H), 6.57 (dd, J=8.9, 2.9 Hz, 2H), 2.96 (s, 12H), 1.67 (s, 9H), 0.64 (s, 3H), 0.60 (s, 3H).
13C NMR (101 MHz, cdcl3) δ 167.8, 166.0, 155.8, 149.4, 136.7, 133.8, 133.3, 131.8, 128.7, 128.3, 126.5, 123.8, 116.6, 113.6, 90.3, 83.3, 40.4, 28.1, 0.4, −1.1.
ESI-MS, positive mode: m/z=529.3 [M+H]+.
HRMS (ESI) calcd for C31H37N2O4Si [M+H]+ 529.2517, found 529.2521.
Trifluoroacetic acid (2 mL) was added dropwise to a solution of compound 14 (67 mg, 0.138 mmol) in DCM (8 mL). The resulting coloured solution was stirred at room temperature for 2 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with toluene to remove excess of trifluoroacetic acid. The residue was lyophilized from 1,4-dioxane-water (1:1). Obtained 74 mg (98%) of pink powder as trifluoroacetic acid salt.
1H NMR (400 MHz, cd3od) δ 8.25 (dd, J=7.8, 1.2 Hz, 1H), 7.82 (t, J=7.8 Hz, 1H), 7.60 (dd, J=7.8, 1.2 Hz, 1H), 7.21 (d, J=9.5 Hz, 2H), 7.08 (dd, J=9.5, 2.4 Hz, 2H), 6.87 (d, J=2.4 Hz, 2H), 3.29 (s, 12H).
13C NMR (101 MHz, cd3od) δ 170.5, 168.6, 159.0, 159.0, 157.0, 136.8, 134.1, 132.8, 132.5, 132.2, 132.0, 131.0, 115.5, 115.2, 97.4, 41.0.
ESI-MS, positive mode: m/z=431.2 [M+H]+.
HRMS (ESI) calcd for C25H23N2O5 [M+H]+ 431.1601, found 431.1606.
Trifluoroacetic acid (2 mL) was added dropwise to a solution of compound 15 (220 mg, 0.321 mmol) in DCM (8 mL). The resulting coloured solution was stirred at room temperature for 3 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with toluene to remove excess of trifluoroacetic acid. The residue was lyophilized from 1,4-dioxane-water (1:1). Obtained 170 mg (98%) of violet powder as trifluoroacetic acid salt.
1H NMR (400 MHz, cd3od) δ 8.17 (dd, J=7.7, 1.1 Hz, 1H), 7.76 (t, J=7.7 Hz, 1H), 7.52 (dd, J=7.7, 1.1 Hz, 1H), 7.11 (d, J=2.2 Hz, 2H), 7.03 (d, J=9.2 Hz, 2H), 6.64 (dd, J=9.2, 2.2 Hz, 2H), 3.06 (s, 6H), 1.81 (s, 3H), 1.68 (s, 3H).
13C NMR (101 MHz, cd3od) δ 170.6, 168.9, 162.5, 159.4, 139.6, 139.5, 136.6, 136.0, 134.2, 131.72, 131.68, 130.57, 122.1, 112.7 (visible in HSQC spectra), 42.7, 35.8, 31.9, 30.1.
ESI-MS, positive mode: m/z=429.2 [M+H]+.
HRMS (ESI) calcd for C26H25N2O4 [M+H]+ 429.1809, found 429.1807.
Trifluoroacetic acid (2 mL) was added dropwise to a solution of compound 16 (230 mg, 0.448 mmol) in DCM (8 mL). The resulting coloured solution was stirred at room temperature for 1 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with toluene to remove excess of trifluoroacetic acid. The residue was lyophilized from 1,4-dioxane-water (1:1). Obtained 251 mg (98%) of dark-violet powder as trifluoroacetic acid salt.
1H NMR (400 MHz, cd3od) δ 8.17 (dd, J=7.8, 1.2 Hz, 1H), 7.77 (t, J=7.8 Hz, 1H), 7.53 (dd, J=7.8, 1.2 Hz, 1H), 7.23 (d, J=2.5 Hz, 2H), 7.09 (d, J=9.4 Hz, 2H), 6.84 (dd, J=9.4, 2.5 Hz, 2H), 3.34 (s, 12H), 1.86 (s, 3H), 1.72 (s, 3H).
13C NMR (101 MHz, cd3od) δ 170.6, 168.9, 158.3, 157.9, 138.9, 136.6, 135.9, 134.2, 131.8, 131.7, 130.6, 122.0, 114.0, 112.1, 43.2, 41.0, 36.2, 32.3.
ESI-MS, positive mode: m/z=457.2 [M+H]+.
HRMS (ESI) calcd for C28H29N2O4 [M+H]+ 457.2122, found 457.2119.
Trifluoroacetic acid (2 mL) was added dropwise to a solution of compound 17 (50 mg, 0.095 mmol) in DCM (8 mL). The resulting coloured solution was stirred at room temperature for 1 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with toluene to remove excess of trifluoroacetic acid. The residue was lyophilized from 1,4-dioxane-water (1:1). Obtained 54 mg (98%) of blue powder as trifluoroacetic acid salt.
1H NMR (400 MHz, d5-pyridine) δ 8.17 (dd, J=7.7, 0.9 Hz, 1H), 7.77 (t, J=7.7 Hz, 1H), 7.54 (dd, J=7.7, 0.9 Hz, 1H), 7.18 (d, J=2.9 Hz, 2H), 7.04 (d, J=9.0 Hz, 2H), 6.54 (dd, J=9.0, 2.9 Hz, 2H), 2.85 (s, 12H), 0.73 (s, 3H), 0.65 (s, 3H).
13C NMR (101 MHz, d5-pyridine) δ 170.1, 169.4, 150.4 (overlapped with pyridine, visible in HMBC spectra), 157.1, 137.4, 135.6, 134.9, 132.3, 129.7, 129.2, 127.0, 124.2 (overlapped with pyridine, visible in HMBC spectra), 117.4, 114.6, 92.3, 40.4, 0.8, −0.8.
ESI-MS, positive mode: m/z=473.2 [M+H]+.
HRMS (ESI) calcd for C27H29N2O4Si [M+H]+ 473.1891, found 473.1890.
A solution of docetaxel (194 mg, 0.24 mmol) in 95% formic acid (2 mL) was stirred at room temperature for 4 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder.
Into a solution of Boc-8-aminooctanoic acid (1.4 eq., 0.336 mmol, 91.7 mg) in MeCN (2 mL) was added HBTU (1.2 eq., 0.288 mmol, 109 mg), followed by DIPEA (4.3 eq., 1 mmol, 100 μL). Mixture was stirred at rt for 5 min and previously obtained des-Boc-docetaxel (0.24 mmol) was added. Mixture was stirred for 1 hour then solvent was removed by rotary evaporator. The residue was re-dissolved in 70% MeCN—H2O mixture, microfiltered through a 0.45 μm PTFE membrane filter and purified by the preparative HPLC (preparative column: Knauer 100 C18, 5 μm, 250×30 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 50:50 isocratic, 5-30 min 50:50 to 100:0 gradient). Fractions containing the product were collected, solvent was removed and obtained residue was lyophilised from 50:50 MeCN:H2O mixture to obtain product as white solid. Obtained 52% 118 mg of white fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 8.36 (d, J=9.0 Hz, 1H), 7.99 (dt, J=7.0, 1.4 Hz, 2H), 7.69 (tt, J=7.4, 1.4 Hz, 1H), 7.60 (t, J=7.5 Hz, 2H), 7.39-7.29 (m, 4H), 7.21 (tt, J=7.2, 1.5 Hz, 1H), 6.73 (t, J=5.7 Hz, 1H), 5.95-5.88 (m, 2H), 5.42 (d, J=7.2 Hz, 1H), 5.27 (dd, J=9.0, 5.9 Hz, 1H), 5.10 (s, 1H), 5.01 (d, J=7.2 Hz, 1H), 4.97-4.88 (m, 2H), 4.58 (s, 1H), 4.41 (t, J=6.3 Hz, 1H), 4.09-3.99 (m, 3H), 3.69 (d, J=7.2 Hz, 1H), 2.87 (q, J=6.7 Hz, 2H), 2.28 (s, 1H), 2.24 (s, 3H), 2.15 (t, J=7.3 Hz, 2H), 1.98 (dd, J=15.3, 9.2 Hz, 1H), 1.82 (dd, J=15.4, 8.9 Hz, 1H), 1.75 (s, 3H), 1.73-1.59 (m, 1H), 1.53 (s, 3H), 1.49-1.43 (m, 2H), 1.36 (s, 9H), 1.34-1.26 (m, 2H), 1.18 (q, J=9.0, 7.1 Hz, 6H), 1.03 (s, 3H), 0.99 (s, 3H).
13C NMR (101 MHz, d6-dmso) δ 209.3, 172.6, 171.9, 169.7, 165.3, 155.5, 139.7, 136.8, 135.9, 133.3, 130.0, 129.6, 128.7, 128.1, 127.14, 127.10, 83.7, 80.3, 77.3, 76.9, 75.5, 74.8, 73.8, 73.6, 70.8, 70.0, 57.0, 55.0, 46.0, 42.9, 39.8, 36.5, 35.4, 35.1, 29.5, 28.6, 28.5, 28.3, 26.5, 26.2, 25.4, 22.4, 20.8, 13.7, 9.8.
ESI-MS, positive mode: m/z=949.5 [M+H]+.
HRMS (ESI) calcd for C51H69N2O15 [M+H]+ 949.4692, found 949.4683.
A solution of cabazitaxel (200 mg, 0.24 mmol) in 95% formic acid (2 mL) was stirred at room temperature for 2 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder.
Into a solution of Boc-8-aminooctanoic acid (91.7 mg, 0.336 mmol, 1.4 eq.,) in MeCN (2 mL) was added HBTU (109 mg, 0.288 mmol, 1.2 eq.), followed by DIPEA (100 μL, 1 mmol, 4.3 eq.). Mixture was stirred at rt for 5 min and previously obtained des-Boc-docetaxel (0.24 mmol) was added. Mixture was stirred for 1 hour then solvent was removed by rotary evaporator. The residue was re-dissolved in 70% MeCN—H2O mixture, microfiltered through a 0.45 μm PTFE membrane filter and purified by the preparative HPLC (preparative column: Knauer 100 C18, 5 μm, 250×30 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 50:50 isocratic, 5-30 min 50:50 to 100:0 gradient). Fractions containing the product were collected, solvent was removed and obtained residue was lyophilised from 50:50 MeCN:H2O mixture to obtain product as white solid. Obtained 69% (162 mg) of white fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 8.37 (d, J=9.1 Hz, 1H), 8.00 (dt, J=7.1, 1.4 Hz, 2H), 7.70 (tt, J=7.4, 2.2 Hz, 1H), 7.61 (t, J=7.4 Hz, 2H), 7.40-7.30 (m, 4H), 7.23 (tt, J=7.4, 1.4 Hz, 1H), 6.74 (t, J=5.7 Hz, 1H), 5.99-5.91 (m, 2H), 5.40 (d, J=7.1 Hz, 1H), 5.30 (dd, J=9.2, 5.8 Hz, 1H), 4.97 (dd, J=9.7, 2.0 Hz, 1H), 4.72 (s, 1H), 4.66 (s, 1H), 4.44 (dd, J=6.8, 5.8 Hz, 1H), 4.04 (s, 2H), 3.77 (dd, J=10.6, 6.5 Hz, 1H), 3.65 (d, J=7.1 Hz, 1H), 3.32 (s, 3H), 3.23 (s, 3H), 2.87 (q, J=6.6 Hz, 2H), 2.72-2.63 (m, 1H), 2.26 (s, 3H), 2.17 (t, J=7.2 Hz, 2H), 1.99 (dd, J=15.4, 9.1 Hz, 1H), 1.92-1.86 (m, 1H), 1.85 (s, 3H), 1.53 (s, 3H), 1.52-1.43 (m, 3H), 1.37 (s, 9H), 1.35-1.30 (m, 2H), 1.24-1.16 (m, 6H), 1.04 (s, 3H), 0.99 (s, 3H).
13C NMR (101 MHz, d6-dmso) δ 204.7, 172.6, 171.9, 169.9, 165.2, 155.5, 139.6, 138.4, 134.9, 133.3, 129.9, 129.6, 128.7, 128.1, 127.1, 83.2, 82.1, 80.3, 80.2, 77.3, 76.9, 75.3, 74.4, 73.6, 70.0, 56.6, 56.6, 56.0, 54.9, 46.4, 43.0, 39.8, 35.4, 34.9, 31.7, 29.5, 28.6, 28.5, 28.3, 26.7, 26.2, 25.4, 22.4, 21.2, 14.0, 10.2.
ESI-MS, positive mode: m/z=977.5 [M+H]+.
HRMS (ESI) calcd for C53H73N2O15 [M+H]+ 977.5005, found 977.4995.
A solution of larotaxel (200 mg, 0.24 mmol) in 95% formic acid (2 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder.
Into a solution of Boc-8-aminooctanoic acid (91.7 mg, 0.336 mmol, 1.4 eq.,) in MeCN (2 mL) was added HBTU (109 mg, 0.288 mmol, 1.2 eq.), followed by DIPEA (100 μL, 1 mmol, 4.3 eq.). Mixture was stirred at rt for 5 min and previously obtained des-Boc-docetaxel (0.24 mmol) was added. Mixture was stirred for 1 hour then solvent was removed by rotary evaporator. The residue was re-dissolved in 70% MeCN—H2O mixture, microfiltered through a 0.45 μm PTFE membrane filter and purified by the preparative HPLC (preparative column: Knauer 100 C18, 5 μm, 250×30 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 50:50 isocratic, 5-30 min 50:50 to 100:0 gradient). Fractions containing the product were collected, solvent was removed and obtained residue was lyophilised from 50:50 MeCN:H2O mixture to obtain product as white solid.
Obtained 57% (133 mg) of white fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 8.34 (d, J=9.2 Hz, 1H), 8.08-8.00 (m, 2H), 7.69 (tt, J=7.4, 1.4 Hz, 1H), 7.61 (t, J=7.5 Hz, 2H), 7.41-7.29 (m, 4H), 7.19 (tt, J=7.4, 1.7 Hz, 1H), 6.73 (t, J=5.7 Hz, 1H), 6.12 (s, 1H), 6.02-5.91 (m, 1H), 5.87 (d, J=6.8 Hz, 1H), 5.44 (d, J=7.7 Hz, 1H), 5.34 (dd, J=9.2, 5.2 Hz, 1H), 4.77 (s, 1H), 4.72 (d, J=3.0 Hz, 1H), 4.49 (dd, J=6.8, 5.2 Hz, 1H), 4.10-3.94 (m, 2H), 3.91 (d, J=7.6 Hz, 1H), 2.86 (q, J=6.5 Hz, 2H), 2.36-2.23 (m, 4H), 2.17-1.87 (m, 9H), 1.74 (d, J=1.4 Hz, 3H), 1.54 (dd, J=7.3, 4.8 Hz, 1H), 1.50-1.41 (m, 2H), 1.36 (s, 9H), 1.35-1.28 (m, 2H), 1.19-1.14 (m, 6H), 1.12 (s, 3H), 1.08 (s, 3H).
13C NMR (101 MHz, d6-dmso) δ 202.0, 172.8, 172.4, 170.2, 169.5, 165.8, 156.0, 140.5, 140.3, 134.0, 133.7, 130.4, 130.1, 129.2, 128.6, 127.5, 127.5, 84.2, 80.0, 79.0, 77.8, 77.7, 75.7, 74.9, 74.0, 70.0, 55.1, 43.0, 40.3, 38.0, 36.1, 35.8, 34.9, 31.7, 29.9, 29.1, 28.9, 28.7, 26.7, 26.4, 26.0, 25.8, 22.4, 21.8, 21.0, 15.2, 14.4.
ESI-MS, positive mode: m/z=973.6 [M+H]+.
HRMS (ESI) calcd for C53H69N2O15 [M+H]+ 973.4692, found 973.4687.
A solution of DTX-C8-NHBoc (22) (14.2 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-TMR-COOH (18) dye TFA salt (5.5 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected DTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis. Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 20:80 isocratic, 5-30 min 20:80 to 70:30 gradient). Additionally purified by flash column chromatography (Interchim Puriflash 12 g, 15 μm normal phase column, gradient 3% to 30% DCM-MeOH), microfiltered through a 0.45 μm PTFE membrane filter and lyophilized from acetonitrile: water mixture. Yield 26% (3.3 mg) of light purple fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.06 (t, J=5.5 Hz, 1H), 8.38 (d, J=9.0 Hz, 1H), 8.02-7.95 (m, 2H), 7.84 (dd, J=7.6, 1.1 Hz, 1H), 7.76 (t, J=7.6 Hz, 1H), 7.67 (tt, J=7.2, 1.3 Hz, 1H), 7.64-7.55 (m, 2H), 7.41-7.28 (m, 4H), 7.25-7.17 (m, 2H), 6.64-6.57 (m, 2H), 6.53-6.43 (m, 4H), 5.99-5.86 (m, 2H), 5.41 (d, J=7.2 Hz, 1H), 5.27 (dd, J=9.0, 5.9 Hz, 1H), 5.09 (d, J=2.6 Hz, 1H), 5.01 (d, J=7.3 Hz, 1H), 4.94 (d, J=2.5 Hz, 1H), 4.90 (dd, J=9.6, 2.2 Hz, 1H), 4.58 (s, 1H), 4.41 (t, J=6.4 Hz, 1H), 4.12-3.96 (m, 3H), 3.68 (d, J=7.1 Hz, 1H), 3.31-3.27 (m, 2H, overlapped with HDO), 2.94 (s, 13H), 2.33-2.26 (m, 1H), 2.23 (s, 3H), 2.17 (t, J=7.3 Hz, 2H), 2.00-1.94 (m, 1H), 1.86-1.78 (m, 1H), 1.75 (s, 3H), 1.69-1.60 (m, 1H), 1.57-1.46 (m, 7H), 1.35-1.25 (m, 6H), 1.03 (s, 3H), 0.98 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.95 129.99), (7.81 130.12), (7.73 135.55), (7.64 133.72), (7.56 129.09), (7.33 128.55), (7.28 127.56), (7.20 125.80), (7.16 127.51), (6.57 129.08), (6.46 98.37), (6.46 109.29), (5.88 70.41), (5.38 75.24), (5.24 55.43), (5.06 74.19), (4.87 84.14), (4.38 74.03), (4.01 71.22), (3.98 75.85), (3.65 46.37), (3.29 39.79), (2.91 40.22), (2.23, 1.63 36.87), (2.20 22.82), (2.14 35.86), (1.97, 1.78 35.53), (1.71 14.09), (1.53 29.31), (1.49 10.24), (1.47 25.85), (1.31 26.89), (1.21 29.04), (1.21 29.22), (0.99 26.94), (0.95 21.26).
ESI-MS, positive mode: m/z=1261.6 [M+H]+.
HRMS (ESI) calcd for C71H81N4O17 [M+H]+ 1261.5591, found 1261.5594.
A solution of CTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-TMR-COOH (18) dye TFA salt (5.5 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected CTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis. Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 20:80 isocratic, 5-30 min 20:80 to 70:30 gradient). Additionally purified by flash column chromatography (Interchim Puriflash 12 g, 15 μm column, gradient 3% to 30% DCM-MeOH), microfiltered through a 0.45 μm PTFE membrane filter and lyophilised from acetonitrile: water mixture. Yield 26% (3.3 mg) of light purple fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.03 (t, J=5.5 Hz, 1H), 8.35 (d, J=9.1 Hz, 1H), 7.95 (d, J=7.0 Hz, 2H), 7.81 (dd, J=7.5, 1.1 Hz, 1H), 7.73 (t, J=7.6 Hz, 1H), 7.67-7.61 (m, 1H), 7.59-7.53 (m, 2H), 7.36-7.28 (m, 4H), 7.22-7.15 (m, 2H), 6.58 (dd, J=9.4, 1.6 Hz, 2H), 6.50-6.42 (m, 4H), 5.97-5.87 (m, 2H), 5.36 (d, J=7.0 Hz, 1H), 5.27 (dd, J=6.9, 2.4 Hz, 1H), 4.92 (d, J=9.1 Hz, 1H), 4.67 (s, 1H), 4.62 (s, 1H), 4.40 (dd, J=6.9, 5.8 Hz, 1H), 3.99 (s, 2H), 3.72 (dd, J=10.9, 6.4 Hz, 1H), 3.60 (d, J=7.1 Hz, 1H), 3.28-3.25 (m, 5H), 3.18 (s, 3H), 2.91 (s, 12H), 2.66-2.58 (m, 1H), 2.22 (s, 3H), 2.15 (t, J=7.8 Hz, 2H), 1.99-1.92 (m, 3H), 1.88-1.81 (m, 1H), 1.80 (s, 3H), 1.54-1.47 (m, 8H), 1.27-1.23 (m, 6H), 1.00 (s, 3H), 0.94 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.95 130.02), (7.80 130.16), (7.74 135.58), (7.64 133.77), (7.56 129.10), (7.32 128.58), (7.30 127.56), (7.20 125.78), (7.18 127.53), (6.57 129.08), (6.46 109.29), (6.46 98.37), (5.91 70.40), (5.35 74.79), (5.26 55.39), (4.93 83.66), (4.67 82.52), (4.39 74.01), (3.99 75.71), (3.72 80.68), (3.60 46.84), (3.29 39.78), (3.27 57.02), (3.18 57.08), (2.91 40.22), (2.63, 1.46 32.13), (2.21 22.82), (2.15 35.84), (1.98, 1.83 35.48), (1.95 26.99), (1.80 14.44), (1.49 10.56), (1.47 25.85), (1.32 26.90), (1.21 29.21), (1.20 31.71), (1.00 27.12), (0.94 21.63).
ESI-MS, positive mode: m/z=1289.6 [M+H]+.
HRMS (ESI) calcd for C73H85N4O17 [M+H]+ 1289.5904, found 1289.5919.
A solution of LTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by H PLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-TMR-COOH (18) dye TFA salt (5.5 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected LTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 20:80 isocratic, 5-30 min 20:80 to 70:30 gradient). Additionally purified by flash column chromatography (Interchim Puriflash 12 g, 15 μm column, gradient 3% to 30% DCM-MeOH), microfiltered through a 0.45 μm PTFE membrane filter and lyophilised from acetonitrile: water mixture. Yield 44% (5.6 mg) of light purple fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.07 (t, J=5.5 Hz, 1H), 8.50 (d, J=9.1 Hz, 1H), 8.02 (d, J=7.2 Hz, 2H), 7.84 (d, J=7.5 Hz, 1H), 7.76 (t, J=7.6 Hz, 1H), 7.68 (dd, J=8.4, 6.1 Hz, 1H), 7.60 (t, J=7.6 Hz, 2H), 7.37-7.30 (m, 4H), 7.23 (d, J=7.5 Hz, 1H), 7.17 (tt, J=7.3, 1.9 Hz, 1H), 6.61 (d, J=9.5 Hz, 2H), 6.50-6.46 (m, 4H), 6.12 (s, 1H), 5.94 (t, J=9.0 Hz, 1H), 5.50-5.33 (m, 2H), 5.32 (dd, J=9.1, 5.4 Hz, 1H), 4.77 (s, 1H), 4.71 (d, J=3.1 Hz, 1H), 4.49 (d, J=5.5 Hz, 1H), 4.05-3.96 (m, 2H), 3.90 (d, J=7.6 Hz, 1H), 3.30-3.27 (m, 2H), 2.94 (s, 12H), 2.32-2.25 (m, 4H), 2.17-1.86 (m, 9H), 1.74 (s, 3H), 1.59-1.44 (m, 5H), 1.35-1.20 (m, 7H), 1.11 (s, 3H), 1.08 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (8.00 130.09), (7.81 130.12), (7.74 135.60), (7.65 133.75), (7.57 129.19), (7.31 128.62), (7.30 127.50), (7.20 125.77), (7.15 127.38), (6.57 129.09), (6.47 109.30), (6.46 98.37), (6.09 75.67), (5.92 69.90), (5.40 79.98), (5.29 55.23), (4.69 84.16), (4.46 73.96), (3.96 74.90), (3.88 37.97), (3.29 39.77), (2.91 38.96), (2.91 40.22), (2.26 22.36), (2.25 26.03), (2.13 35.83), (2.09 20.99), (1.81 14.82), (1.71 14.39), (1.52 29.37), (1.45 25.88), (1.30 26.91), (1.27 29.01), (1.21 29.10), (1.15 31.63), (1.08 21.82), (1.05 26.34).
ESI-MS, positive mode: m/z=1285.7 [M+H]+.
HRMS (ESI) calcd for C73H81N4O17 [M+H]+ 1285.5591, found 1285.5591.
A solution of LTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain a white powder, which was used further without any additional purifications.
The 4-580CP-COOH (19) dye TFA salt (5.4 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected LTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 30:70 isocratic, 5-30 min 30:70 to 70:30 gradient). Additionally purified by flash column chromatography (Interchim Puriflash 12 g, 15 μm column, gradient 2% to 15% DCM-MeOH) and lyophilised from acetonitrile: water mixture. Yield 38% (4.9 mg) of violet fluffy solid.
1H NMR (500 MHz, d6-dmso) δ 9.14 (t, J=5.5 Hz, 1H), 8.37 (d, J=9.2 Hz, 1H), 8.03 (d, J=7.1 Hz, 2H), 7.78 (dd, J=7.5, 1.0 Hz, 1H), 7.71-7.65 (m, 2H), 7.60 (t, J=7.6 Hz, 2H), 7.38-7.31 (m, 4H), 7.17 (tt, J=7.0, 1.6 Hz, 1H), 7.05 (dd, J=7.7, 1.0 Hz, 1H), 6.76 (d, J=2.4 Hz, 2H), 6.45 (d, J=8.6 Hz, 2H), 6.41-6.36 (m, 2H), 6.12 (s, 1H), 5.96 (t, J=8.9 Hz, 1H), 5.91-5.80 (m, 3H), 5.43 (d, J=7.7 Hz, 1H), 5.34 (dd, J=9.2, 5.2 Hz, 1H), 4.78 (s, 1H), 4.72 (d, J=4.1 Hz, 1H), 4.49 (dd, J=6.8, 5.3 Hz, 1H), 4.05-3.97 (m, 2H), 3.91 (d, J=7.6 Hz, 1H), 3.30-3.16 (m, 2H), 2.70 (s, 3H), 2.69 (s, 3H), 2.31-2.25 (m, 4H), 2.18-1.88 (m, 9H), 1.75 (s, 3H), 1.74 (s, 3H), 1.65 (s, 3H), 1.57-1.45 (m, 5H), 1.37-1.23 (m, 7H), 1.11 (s, 3H), 1.08 (s, 3H).
1H-13C NMR ((500, 126) MHz, d6-dmso) δ (8.03 129.69), (7.77 129.15), (7.69 134.93), (7.68 133.36), (7.60 128.78), (7.35 128.15), (7.33 127.07), (7.17 127.04), (7.06 125.16), (6.75 107.95), (6.46 128.47), (6.38 111.40), (6.11 75.23), (5.95 69.56), (5.44 79.53), (5.34 54.71), (4.71 83.75), (4.49 73.54), (4.02, 3.99 74.38), (3.91 37.53), (3.32 39.34), (2.69 29.56), (2.31, 1.95 25.56), (2.30 21.95), (2.16 35.41), (2.12 20.57), (2.09, 1.89 35.55), (2.03, 1.53 14.88), (1.75 33.25), (1.73 13.98), (1.65 34.56), (1.55 28.92), (1.47 25.46), (1.36 28.30), (1.33 26.48), (1.23 28.88), (1.23 28.57), (1.11 21.41), (1.07 25.91).
ESI-MS, positive mode: m/z=1283.7 [M+H]+.
HRMS (ESI) calcd for C74H82N4O16 [M+H]+ 1283.5799, found 1283.5796
A solution of DTX-C8-NHBoc (22) (14.2 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-610CP-COOH (20) dye TFA salt (5.7 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected DTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 30:70 isocratic, 5-30 min 30:70 to 100:0 gradient). Lyophilised from acetonitrile: water mixture. Yield 42% (5.4 mg) of slightly blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.11 (t, J=5.5 Hz, 1H), 8.37 (d, J=9.0 Hz, 1H), 8.03-7.95 (m, 2H), 7.78 (dd, J=7.5, 1.0 Hz, 1H), 7.72-7.64 (m, 2H), 7.59 (t, J=7.4 Hz, 2H), 7.39-7.30 (m, 4H), 7.20 (tt, J=7.1, 1.4 Hz, 1H), 7.04 (d, J=7.7 Hz, 1H), 6.91 (s, 2H), 6.63-6.52 (m, 4H), 6.03-5.82 (m, 2H), 5.41 (d, J=7.3 Hz, 1H), 5.28 (dd, J=9.1, 5.8 Hz, 1H), 5.10 (s, 1H), 5.01 (s, 1H), 4.90 (dd, J=9.8, 1.6 Hz, 1H), 4.58 (s, 1H), 4.41 (t, J=5.0 Hz, 1H), 4.10-3.96 (m, 3H), 3.68 (d, J=7.2 Hz, 1H), 3.31-3.27 (m, 2H), 2.94 (s, 12H), 2.32-2.25 (m, 1H), 2.24 (s, 3H), 2.17 (t, J=7.3 Hz, 2H), 1.97 (dd, J=15.1, 9.6 Hz, 1H), 1.86-1.80 (m, 4H), 1.75 (s, 3H), 1.72 (s, 3H), 1.69-1.63 (m, 1H), 1.61-1.55 (m, 2H), 1.52 (s, 3H), 1.52-1.47 (m, 2H), 1.38-1.25 (m, 6H), 1.03 (s, 3H), 0.98 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.95 130.00), (7.74 129.59), (7.65 135.43), (7.64 133.73), (7.56 129.09), (7.33 128.57), (7.29 127.57), (7.17 127.53), (7.01 125.46), (6.88 109.57), (6.54 112.20), (6.53 128.80), (5.88 70.45), (5.38 75.26), (5.25 55.43), (5.06 74.19), (4.86 84.14), (4.38 74.02), (4.02 71.22), (3.99 75.89), (3.65 46.41), (3.30 39.78), (2.90 40.44), (2.24, 1.62 36.90), (2.21 22.82), (2.14 35.86), (1.94, 1.78 35.44), (1.79 34.01), (1.72 14.09), (1.68 34.96), (1.54 29.35), (1.49 10.24), (1.47 25.86), (1.32 26.91), (1.24 29.03), (1.20 29.22), (1.00 26.94), (0.95 21.26).
ESI-MS, positive mode: m/z=1287.6 [M+H]+.
HRMS (ESI) calcd for C74H87N4O16 [M+H]+ 1287.6112, found 1287.6110.
A solution of CTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-610CP-COOH (20) dye TFA salt (5.7 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected CTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 30:70 isocratic, 5-30 min 30:70 to 100:0 gradient). Lyophilised from acetonitrile: water mixture.
Yield 46% (6.0 mg) of light blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.09 (t, J=5.5 Hz, 1H), 8.37 (d, J=9.0 Hz, 1H), 7.98-7.91 (m, 2H), 7.75 (dd, J=7.5, 1.0 Hz, 1H), 7.68-7.61 (m, 2H), 7.61-7.51 (m, 2H), 7.36-7.26 (m, 4H), 7.18 (tt, J=7.1, 1.6 Hz, 1H), 7.01 (dd, J=7.7, 1.0 Hz, 1H), 6.88 (d, J=2.0 Hz, 2H), 6.60-6.49 (m, 4H), 6.00-5.87 (m, 2H), 5.36 (d, J=7.1 Hz, 1H), 5.26 (dd, J=9.1, 5.8 Hz, 1H), 4.92 (dd, J=9.6, 2.1 Hz, 1H), 4.68 (s, 1H), 4.62 (s, 1H), 4.40 (t, J=6.2 Hz, 1H), 3.99 (s, 2H), 3.72 (dd, J=10.6, 6.6 Hz, 1H), 3.60 (d, J=7.1 Hz, 1H), 3.34-3.31 (m, 2H), 3.27 (s, 3H), 3.18 (s, 3H), 2.91 (s, 12H), 2.69-2.57 (m, 1H), 2.21 (s, 3H), 2.15 (t, J=7.3 Hz, 2H), 1.97-1.91 (m, 1H), 1.88-1.82 (m, 1H), 1.80 (s, 3H), 1.79 (s, 3H), 1.69 (s, 3H), 1.56-1.43 (m, 8H), 1.37-1.23 (m, 6H), 1.00 (s, 3H), 0.94 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.95 130.01), (7.75 129.60), (7.66 135.42), (7.64 133.80), (7.56 129.10), (7.33 128.58), (7.30 127.57), (7.18 127.54), (7.01 125.47), (6.88 109.55), (6.53 112.18), (6.53 128.79), (5.92 70.41), (5.36 74.79), (5.26 55.41), (4.91 83.63), (4.67 82.52), (4.40 74.01), (3.99 75.70), (3.72 80.67), (3.60 46.85), (3.30 39.78), (3.27 57.03), (3.18 57.07), (2.90 40.41), (2.62, 1.46 32.14), (2.21 22.81), (2.15 35.84), (1.94, 1.84 35.26), (1.80 14.44), (1.79 34.01), (1.68 34.94), (1.54 29.33), (1.49 10.58), (1.48 25.84), (1.32 26.90), (1.24 29.05), (1.22 29.13), (1.00 27.12), (0.94 21.63).
ESI-MS, positive mode: m/z=1315.6 [M+H]+.
HRMS (ESI) calcd for C76H91N4016 [M+H]+ 1315.6425, found 1315.6409.
A solution of LTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-610CP-COOH (20) dye TFA salt (5.7 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected LTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 30:70 isocratic, 5-30 min 30:70 to 100:0 gradient). Lyophilised from acetonitrile: water mixture.
Yield 35% (4.7 mg) of light blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.09 (t, J=5.5 Hz, 1H), 8.37 (d, J=8.8 Hz, 1H), 8.04-7.96 (m, 2H), 7.75 (d, J=7.4 Hz, 1H), 7.70-7.61 (m, 2H), 7.57 (t, J=7.6 Hz, 2H), 7.37-7.25 (m, 4H), 7.14 (tt, J=7.0, 1.9 Hz, 1H), 7.01 (d, J=7.6 Hz, 1H), 6.88 (d, J=2.1 Hz, 2H), 6.59-6.48 (m, 4H), 6.09 (s, 1H), 6.00-5.81 (m, 2H), 5.40 (d, J=7.6 Hz, 1H), 5.31 (dd, J=9.1, 5.3 Hz, 1H), 4.74 (s, 1H), 4.68 (d, J=3.7 Hz, 1H), 4.46 (d, J=3.8 Hz, 1H), 3.97 (dd, J=20.5, 8.5 Hz, 2H), 3.88 (d, J=7.6 Hz, 1H), 3.28-3.26 (m, 2H), 2.91 (s, 12H), 2.27 (s, 4H), 2.15-1.84 (m, 9H), 1.79 (s, 3H), 1.71 (s, 3H), 1.69 (s, 3H), 1.55-1.44 (m, 5H), 1.33-1.21 (m, 7H), 1.08 (s, 3H), 1.05 (s, 3H).
ESI-MS, positive mode: m/z=1311.6 [M+H]+.
HRMS (ESI) calcd for C76H87N4O16 [M+H]+ 1311.6112, found 1311.6085.
A solution of DTX-C8-NHBoc (22) (14.2 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-SiR—COOH (20) dye TFA salt (5.9 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected DTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 40:60 isocratic, 5-30 min 40:60 to 100:0 gradient) and lyophilised from acetonitrile and water mixture. Yield 29% (3.8 mg) of slightly blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 9.01 (t, J=5.5 Hz, 1H), 8.38 (d, J=9.0 Hz, 1H), 8.03-7.95 (m, 2H), 7.80-7.72 (m, 2H), 7.67 (tt, J=7.3, 1.5 Hz, 1H), 7.59 (t, J=7.7, 7.2 Hz, 2H), 7.40-7.29 (m, 4H), 7.27-7.22 (m, 1H), 7.22-7.16 (m, 1H), 7.00 (d, J=2.8 Hz, 2H), 6.70 (dd, J=8.9, 1.0 Hz, 2H), 6.67-6.60 (m, 2H), 5.96-5.87 (m, 2H), 5.41 (d, J=7.2 Hz, 1H), 5.27 (dd, J=9.0, 5.9 Hz, 1H), 5.09 (d, J=2.5 Hz, 1H), 5.01 (d, J=7.2 Hz, 1H), 4.94 (d, J=2.5 Hz, 1H), 4.90 (dd, J=9.7, 2.2 Hz, 1H), 4.58 (s, 1H), 4.41 (t, J=6.4 Hz, 1H), 4.10-3.96 (m, 3H), 3.68 (d, J=7.1 Hz, 1H), 3.30-3.25 (m, 2H), 2.92 (s, 12H), 2.34-2.25 (m, 1H), 2.24 (s, 3H), 2.17 (t, J=7.4 Hz, 2H), 2.02-1.92 (m, 1H), 1.81 (dd, J=15.3, 9.0 Hz, 1H), 1.75 (s, 3H), 1.66 (dd, J=15.4, 10.1 Hz, 1H), 1.58-1.45 (m, 7H), 1.39-1.24 (m, 6H), 1.03 (s, 3H), 0.98 (s, 3H), 0.62 (s, 3H), 0.52 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.98 130.02), (7.76 129.42), (7.76 134.93), (7.67 133.74), (7.60 129.08), (7.36 128.57), (7.32 127.56), (7.24 125.80), (7.20 127.58), (7.00 116.66), (6.69 128.36), (6.64 114.05), (5.91 70.43), (5.42 75.21), (5.28 55.43), (5.10 74.21), (4.90 84.11), (4.42 73.99), (4.06 71.20), (4.03 75.88), (3.68 46.40), (3.30 39.76), (2.92 40.22), (2.27, 1.66 36.90), (2.24 22.83), (2.17 35.85), (1.97, 1.81 35.53), (1.75 14.10), (1.54 29.30), (1.53 10.24), (1.50 25.85), (1.34 26.88), (1.26 29.03), (1.25 29.06), (1.03 26.94), (0.98 21.26), (0.62-0.75), (0.52 0.41).
ESI-MS, positive mode: m/z=1303.6 [M+H]+.
HRMS (ESI) calcd for C73H87N4O16Si [M+H]+ 1303.5881, found 1303.5882.
solution of CTX-C8-NHBoc (23) (14.6 mg, 0.015 mmol) in 95% formic acid (1 mL) was stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-SiR—COOH (20) dye TFA salt (5.9 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected CTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 40:60 isocratic, 5-30 min 40:60 to 100:0 gradient) and lyophilised from acetonitrile and water mixture. Yield 51% (6.8 mg) of slightly blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 8.99 (t, J=5.5 Hz, 1H), 8.34 (d, J=9.0 Hz, 1H), 7.95 (d, J=6.9 Hz, 2H), 7.74-7.71 (m, 2H), 7.66-7.61 (m, 1H), 7.56 (t, J=7.5 Hz, 2H), 7.32 (ddd, J=15.2, 8.2, 6.8 Hz, 4H), 7.21 (h, J=2.9, 1.9 Hz, 2H), 6.97 (d, J=2.8 Hz, 2H), 6.67 (dd, J=9.0, 1.2 Hz, 2H), 6.63-6.59 (m, 2H), 5.92 (dd, J=8.5, 6.4 Hz, 2H), 5.36 (d, J=7.1 Hz, 1H), 5.26 (dd, J=9.1, 5.8 Hz, 1H), 4.92 (d, J=9.5 Hz, 1H), 4.67 (s, 1H), 4.62 (s, 1H), 4.40 (dd, J=6.9, 5.8 Hz, 1H), 3.99 (s, 2H), 3.72 (dd, J=10.6, 6.5 Hz, 1H), 3.60 (d, J=7.1 Hz, 1H), 3.27 (s, 3H), 3.27-3.24 (m, 2H), 3.18 (s, 3H), 2.89 (s, 12H), 2.69-2.56 (m, 1H), 2.22 (s, 3H), 2.15 (t, J=7.4 Hz, 2H), 1.94 (dd, J=15.3, 8.9 Hz, 1H), 1.87-1.81 (m, 1H), 1.80 (s, 3H), 1.54-1.43 (m, 8H), 1.34-1.17 (m, 6H), 1.00 (s, 3H), 0.94 (s, 3H), 0.59 (s, 3H), 0.49 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.95 129.99), (7.73 129.43), (7.73 134.94), (7.64 133.81), (7.56 129.12), (7.33 128.58), (7.30 127.56), (7.21 125.83), (7.18 127.50), (6.97 116.66), (6.66 128.36), (6.61 114.05), (5.92 70.41), (5.36 74.79), (5.26 55.36), (4.92 83.65), (4.67 82.53), (4.39 74.02), (3.99 75.71), (3.73 80.65), (3.60 46.85), (3.27 57.03), (3.27 39.77), (3.18 57.09), (2.88 40.22), (2.62, 1.47 32.13), (2.22 22.83), (2.14 35.85), (1.94, 1.85 35.32), (1.80 14.45), (1.51 29.28), (1.49 10.58), (1.47 25.86), (1.30 26.87), (1.22 29.10), (1.21 29.15), (1.00 27.13), (0.94 21.64), (0.59-0.75), (0.49 0.41).
ESI-MS, positive mode: m/z=1331.6 [M+H]+.
HRMS (ESI) calcd for C75H91N4O16Si [M+H]+ 1331.6194, found 1331.6184.
A solution of LTX-C8-NHBoc (23) (14.6 mg, stirred at room temperature for 1 h. Reaction progress was monitored by HPLC analysis. Once reaction was complete, formic acid was evaporated on rotary evaporator and residue was dissolved in water and lyophilised to obtain white powder, which was used further without any additional purifications.
The 4-SiR—COOH (20) dye TFA salt (5.7 mg, 0.01 mmol, 1 eq), DIPEA (52 μL, 0.3 mmol, 30 eq.) and HBTU (4.5 mg, 0.012 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min. A solution of previously obtained deprotected LTX-C8-NH2 derivative (0.015 mmol, 1.5 eq) in MeCN and 10 μL of DIPEA were added to the reaction mixture and stirring continued for 1 hour. Reaction was monitored by HPLC analysis.
Purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 40:60 isocratic, 5-30 min 40:60 to 100:0 gradient). Lyophilised from acetonitrile: water mixture.
Yield 41% (5.4 mg) of light blue fluffy solid.
1H NMR (400 MHz, d6-dmso) δ 8.99 (t, J=5.5 Hz, 1H), 8.35 (d, J=9.2 Hz, 1H), 8.00 (dd, J=7.0, 1.4 Hz, 2H), 7.78-7.68 (m, 2H), 7.65 (tt, J=7.3, 1.3 Hz, 1H), 7.57 (t, J=7.4 Hz, 2H), 7.37-7.25 (m, 4H), 7.22 (dd, J=5.7, 3.0 Hz, 1H), 7.14 (tt, J=7.2, 1.9 Hz, 1H), 6.97 (d, J=2.8 Hz, 2H), 6.67 (dd, J=8.8, 0.8 Hz, 2H), 6.60 (ddd, J=9.0, 2.8, 1.4 Hz, 2H), 6.09 (s, 1H), 5.99-5.84 (m, 2H), 5.41 (d, J=7.7 Hz, 1H), 5.31 (dd, J=9.2, 5.2 Hz, 1H), 4.74 (s, 1H), 4.68 (d, J=4.1 Hz, 1H), 4.46 (t, J=5.3 Hz, 1H), 3.98 (dd, J=20.6, 8.6 Hz, 2H), 3.88 (d, J=7.6 Hz, 1H), 3.25 (d, J=6.7 Hz, 2H), 2.89 (s, 12H), 2.27 (s, 4H), 2.15-1.83 (m, 9H), 1.71 (s, 3H), 1.55-1.40 (m, 5H), 1.31-1.16 (m, 7H), 1.08 (s, 3H), 1.05 (s, 3H), 0.59 (s, 3H), 0.49 (s, 3H).
1H-13C NMR ((400, 101) MHz, d6-dmso) δ (7.99 130.06), (7.74 129.45), (7.73 134.93), (7.64 133.75), (7.57 129.16), (7.30 127.71), (7.22 125.83), (7.14 127.47), (6.97 116.67), (6.66 128.37), (6.65 114.02), (6.61 114.03), (6.09 75.65), (5.92 69.96), (5.40 79.98), (5.31 55.15), (4.68 84.19), (4.46 73.97), (3.97 74.81), (3.88 37.96), (3.26 39.74), (2.88 40.21), (2.27 22.36), (2.24, 1.96 26.00), (2.12 35.84), (2.09 20.98), (2.07, 1.87 36.02), (1.99, 1.51 15.14), (1.71 14.39), (1.50 29.29), (1.44 25.87), (1.28 26.86), (1.22 26.99), (1.20 29.11), (1.14 31.67), (1.08 21.82), (1.05 26.34), (0.59-0.76), (0.49 0.41).
ESI-MS, positive mode: m/z=1327.6 [M+H]+.
HRMS (ESI) calcd for C75H87N4O16Si [M+H]+ 1327.5881, found 1327.5895.
The free base of Hoechst 33258 was prepared by dissolving commercial Hoechst 33258 trihydrochloride (500 mg, 0.936 mmol) in H2O (30 mL) and adding a solution of K2CO3 (388 mg, 2.81 mmol, 3 eq.) in H2O (10 mL). The precipitate thus formed was isolated by filtration, washed with H2O and lyophilised from water 1,4-dioxane mixture. The resulting Hoechst 33258 base (397 mg, 0.936 mmol, 1 eq.) was suspended in dry DMF (1 mL). K2CO3 (388 mg, 2.81 mmol, 3 eq.) was added followed by 4-(Boc-amino)butyl bromide (283 mg, 1.12 mmol, 1.2 eq.). The reaction was heated at 60° C. for 14 h. The reaction mixture was then cooled to room temperature, DMF was evaporated under reduced pressure. The residue was suspended in DCM and deposited on celite by evaporating the solvent. Product was isolated by flash column chromatography (Büchi Reveleris HP silica 40 g; gradient 20% to 90% CH2Cl2—CH2Cl2:MeOH:NH3(aq) [9:1:0.2]) as yellow solid, yield 306 mg (55%).
1H NMR (600 MHz, CD3OD) δ 8.22 (d, J=1.6 Hz, 1H), 8.01 (d, J=8.8 Hz, 2H), 7.92 (dd, J=8.4, 1.6 Hz, 1H), 7.66 (d, J=8.4 Hz, 1H), 7.50 (d, J=8.7 Hz, 1H), 7.13 (d, J=2.3 Hz, 1H), 7.01-7.07 (m, 3H), 4.03 (t, J=6.4 Hz, 2H), 3.23-3.33 (m, 4H), 3.11 (t, J=7.0 Hz, 2H), 2.79-2.84 (m, 4H), 2.48 (s, 3H), 1.80 (p, J=7.2 Hz, 2H), 1.65 (p, J=7.2 Hz, 2H), 5 1.43 (s, 9H).
13C NMR (126 MHz, CD3OD) b 169.6, 162.4, 158.4, 155.2, 153.7, 149.2, 148.3, 140.4, 136.0, 129.5, 125.5, 122.8, 122.4, 116.5, 116.3, 116.0, 113.8, 102.5, 79.9, 68.9, 56.0, 51.4, 45.6, 41.1, 28.8, 27.7.
HRMS (ESI) calcd for C34H42N7O3 [M+H]+ 596.3344, found 596.3340.
Trifluoroacetic acid (0.2 mL) was added dropwise to a solution of Hoechst-C4-NHBoc (35) (16.5 mg, 0.0276 mmol) in CH2Cl2 (0.8 mL). The resulting intense yellow solution was stirred at room temperature for 3 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with MeOH to remove excess trifluoroacetic acid. The residue was lyophilized from aqueous dioxane. Deprotected product obtained quantatively as trifluoroacetic acid salt ([Hoechst-(CH2)4NH3]4+[CF3COO−]4) as yellow solid. Compound used in further step without additional purification.
4-TMR-COOH-TFA salt (10 mg, 0.0184 mmol, 1 eq.), EDCl-HCl (8.4 mg, 0.0276 mmol, 1.5 eq.) DMAP (0.11 mmol, 13.4 mg, 6 eq.) and previously obtained [Hoechst-(CH2)4NH3]4+[CF3COO−]4 (26 mg, 0.0276 mmol, 1.5 eq.) were dissolved in 1 mL of dry DMF and stirred for 1 hour. Then DMF was removed at rt under reduced pressure. The product was purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 20:80 isocratic, 5-30 min 20:80 to 70:30 gradient). Water acetonitrile mixture was removed by rotary evaporator and product was purified one more time by flash chromatography (silica-gel cartridge: Interchim Puriflash 12 g, 15 μm column, gradient 20% to 80% CH2Cl2—CH2Cl2:MeOH:NH3(aq) [85:15:2]). Solvents were removed and a product was lyophilised from water acetonitrile mixture to obtain 9 mg (54%) of purple solid.
1H NMR (400 MHz, CD3OD+CF3COOD) δ 8.58 (dd, J=1.7, 0.7 Hz, 1H), 8.25 (dd, J=8.7, 1.7 Hz, 1H), 8.18 (d, J=9.0 Hz, 2H), 8.07 (dd, J=8.7, 0.7 Hz, 1H), 7.83-7.79 (m, 2H), 7.76 (d, J=9.1 Hz, 1H), 7.51 (dd, J=6.2, 2.7 Hz, 1H), 7.44 (dd, J=9.2, 2.2 Hz, 1H), 7.36 (d, J=2.2 Hz, 1H), 7.29 (d, J=9.0 Hz, 2H), 7.23 (d, J=9.5 Hz, 2H), 7.07 (dd, J=9.5, 2.5 Hz, 2H), 6.97 (d, J=2.4 Hz, 2H), 4.22 (t, J=6.2 Hz, 2H), 3.97 (d, J=13.2 Hz, 2H), 3.68 (d, J=12.1 Hz, 2H), 3.49 (t, J=6.9 Hz, 2H), 3.36 (d, J=11.3 Hz, 2H), 3.30 (s, 12H), 3.19 (d, J=11.9 Hz, 2H), 3.00 (s, 3H), 2.03-1.94 (m, 2H), 1.87 (dd, J=8.7, 6.0 Hz, 2H).
13C NMR (101 MHz, CD3OD+CF3COOD) δ 171.1, 169.2, 165.6, 159.1, 158.8, 154.3, 151.1, 149.5, 139.2, 134.6, 133.9, 133.7, 132.5, 131.9, 131.5, 130.2, 128.0, 126.3, 121.7, 120.9, 120.0, 118.1, 117.5, 117.2, 116.4, 115.7, 115.5, 115.2, 115.0, 114.6, 112.4, 101.0, 97.5, 69.5, 54.6, 48.4 (visible in HSQC), 43.6, 41.0, 40.7, 27.5, 26.9.
ESI-MS, positive mode: m/z=908.5 [M+H]+.
HRMS (ESI) calcd for C54H54N9O5 [M+H]+ 908.4242, found 908.4230.
Trifluoroacetic acid (0.2 mL) was added dropwise to a solution of Hoechst-C4-NHBoc (35) (16.5 mg, 0.0276 mmol) in CH2Cl2 (0.8 mL). The resulting intense yellow solution was stirred at room temperature for 3 h. The reaction mixture was then evaporated to dryness, the residue was re-evaporated three times with MeOH to remove excess trifluoroacetic acid. The residue was lyophilized from aqueous dioxane. Deprotected product obtained quantatively as trifluoroacetic acid salt ([Hoechst-(CH2)4NH3]4+[CF3COO−]4) as yellow solid. Compound used in further step without additional purification.
4-580CP-COOH-TFA salt (0.0184 mmol, 10 mg, 1 eq.), EDCl-HCl (0.0276 mmol, 8.4 mg, 1.5 eq.) DMAP (0.11 mmol, 13.4 mg, 6 eq.) and [Hoechst-(CH2)4NH3]4+[CF3COO−]4 (0.0276, 26 mg, 1.5 eq.) were dissolved in 1 mL of dry DMF and stirred for 1 hour. Then DMF was removed at rt under reduced pressure. The product was purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 20:80 isocratic, 5-30 min 20:80 to 70:30 gradient). Water acetonitrile mixture was removed by rotary evaporator and product was purified one more time by flash chromatography (silica-gel cartridge: Interchim Puriflash 12 g, 15 μm column, gradient 20% to 80% CH2Cl2—CH2Cl2:MeOH:NH3(aq) [85:15:2]). Solvents were removed and a product was lyophilised from water acetonitrile mixture to obtain 6 mg (36%) of dark-violet solid.
1H NMR (400 MHz, CD3OD+CF3COOD) δ 8.39 (s, 1H), 8.17-8.04 (m, 3H), 7.95 (d, J=8.6 Hz, 1H), 7.86-7.78 (m, 2H), 7.76 (d, J=8.9 Hz, 1H), 7.48-7.39 (m, 2H), 7.36 (s, 1H), 7.21-7.12 (m, 4H), 7.09 (d, J=9.1 Hz, 2H), 6.68 (dd, J=9.1, 2.3 Hz, 2H), 4.13 (t, J=5.7 Hz, 2H), 3.98 (d, J=13.1 Hz, 2H), 3.77-3.71 (m, 2H), 3.52 (t, J=6.0 Hz, 2H), 3.35 (d, J=10.6 Hz, 2H), 3.27 (d, J=13.3 Hz, 2H), 3.07 (s, 6H), 3.03 (s, 3H), 1.99-1.85 (m, 4H), 1.83 (s, 3H), 1.70 (s, 3H).
13C NMR (101 MHz, CD3OD+CF3COOD) b 169.7, 168.6, 162.7, 161.7, 161.4, 157.3, 156.7, 153.7, 149.2, 148.7, 137.4, 136.8, 136.7, 133.2, 132.0, 131.1, 130.3, 129.2, 128.1, 126.9, 124.7, 123.2, 120.7, 118.6, 118.1, 115.2, 115.2, 114.3, 113.7, 112.7, 111.0, 99.7, 67.8, 53.2, 47.1, 42.3, 41.1, 39.4, 34.2, 30.8, 28.9, 26.2, 25.5.
ESI-MS, positive mode: m/z=906.4 [M+H]+.
HRMS (ESI) calcd for C55H56N9O4 [M+H]+ 906.4450, found 906.4455.
4-610CP-COOH (20) TFA salt (0.0175 mmol, 10 mg, 1 eq.), DIPEA (15 μL, 0.0875 mmol, 5 eq.) and HBTU (0.021 mmol, 8.0 mg, 1.2 eq.) were dissolved in 400 μL of dry DMSO and stirred at room temperature for 15 min. A solution of 6-aminocaproic acid (0.0175 mmol, 2.3 mg, 1 eq.) in 200 μL of DMSO:H2O mixture (1:1) was added to the reaction mixture and stirring continued for 1 hour. Reaction mixture was neutralised with formic acid and product was purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 40:60 isocratic, 5-30 min 40:60 to 80:20 gradient). Product lyophilised from acetonitrile: water mixture to obtain 7 mg (70%) of blue powder.
1H NMR (400 MHz, CD3CN) δ 9.74 (t, J=5.1 Hz, 1H), 8.28 (dd, J=7.7, 1.0 Hz, 1H), 7.72 (t, J=7.7 Hz, 1H), 7.08 (dd, J=7.7, 1.0 Hz, 1H), 6.96 (d, J=2.3 Hz, 2H), 6.64-6.53 (m, 4H), 3.49 (q, J=7.0 Hz, 2H), 2.97 (s, 13H), 2.31 (t, J=7.4 Hz, 2H), 2.20 (s, 1H), 1.86 (s, 3H), 1.75 (s, 3H), 1.73-1.61 (m, 4H), 1.56-1.44 (m, 2H).
13C NMR (101 MHz, CD3CN) b 175.08, 172.55, 164.64, 158.41, 152.15, 147.76, 136.10, 134.66, 132.21, 129.55, 127.07, 123.70, 119.05, 112.78, 110.31, 40.67, 40.59, 39.27, 35.31, 34.14, 33.58, 29.72, 27.25, 25.32.
ESI-MS, positive mode: m/z=570.3 [M+H]+.
HRMS (ESI) calcd for C34H40N3O5[M+H]+ 570.2962, found 570.2965.
4-610CP-C5—COOH (37) (0.021 mmol, 12.0 mg), TSTU (0.0274 mmol, 8.1 mg) and DIPEA (36 μL, 0.2064 mmol) were dissolved in 500 μL of MeCN and stirred for 1 hour. Then MeCN was removed by rotary evaporator and obtained product was purified by flash chromatography (silica-gel cartridge: Interchim Puriflash 12 g, 15 μm column, gradient 20% to 100% DCM-EtOAc). The solvents were removed and the obtained NHS ester was redissolved in 1,4-dioxane, microfiltered through a 0.45 μm PTFE membrane filter and lyophilized to obtain 10 mg (71%) of blue powder.
1H NMR (400 MHz, Acetonitrile-d3) δ 9.71 (s, 1H), 8.28 (dd, J=7.7, 1.0 Hz, 1H), 7.74 (t, J=7.7 Hz, 1H), 7.09 (dd, J=7.7, 1.0 Hz, 1H), 6.97 (d, J=2.3 Hz, 2H), 6.71-6.54 (m, 4H), 3.51 (td, J=6.9, 5.4 Hz, 2H), 2.99 (s, 12H), 2.75 (s, 4H), 2.67 (t, J=7.4 Hz, 2H), 1.87 (s, 3H), 1.85-1.77 (m, 2H), 1.76 (s, 3H), 1.76-1.68 (m, 2H), 1.63-1.54 (m, 2H).
ESI-MS, positive mode: m/z=667.2 [M+H]+.
The 4-610CP-C6—NHS (39) (2.7 mg, 0.0041 mmol) ester was dissolved in 400 μL of MeCN followed by the addition of deprotected des-bromo-des-methyl-Lys-jasplakinolide (0.00342 mmol, 2.3 mg) (Tannert, R., Milroy, L. G., Ellinger, B., Hu, T. S., Arndt, H. D., Waldmann, H., J Am Chem Soc, 2010, 132 (9), 3063-3077) and DIPEA (12 μL, 0.0688 mmol). The reaction mixture was stirred for 1 hour and purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 30:70 isocratic, 5-30 min 30:70 to 100:0 gradient). Product lyophilised from acetonitrile: water mixture to obtain 1.8 mg (48%) of light blue powder.
1H NMR (400 MHz, d6-dmso) δ 10.79 (d, J=1.8 Hz, 1H), 9.27 (s, 1H), 9.11 (t, J=5.5 Hz, 1H), 8.61 (d, J=8.8 Hz, 1H), 7.75 (d, J=7.6 Hz, 1H), 7.70-7.60 (m, 4H), 7.26 (d, J=8.1 Hz, 1H), 7.10 (d, J=8.6 Hz, 2H), 7.03 (d, J=2.3 Hz, 1H), 7.02-6.97 (m, 2H), 6.92 (t, J=7.2 Hz, 1H), 6.88 (d, J=2.1 Hz, 2H), 6.67 (d, J=8.5 Hz, 2H), 6.58-6.50 (m, 4H), 5.49 (dd, J=11.3, 5.1 Hz, 1H), 5.22-5.11 (m, 1H), 4.89 (t, J=7.1 Hz, 1H), 4.64 (h, J=6.4 Hz, 1H), 4.58-4.44 (m, 1H), 3.35-3.31 (m, 2H), 3.08-2.96 (m, 4H), 2.91 (s, 12H), 2.89-2.71 (m, 3H), 2.65 (dd, J=14.7, 11.3 Hz, 1H), 2.55 (dd, J=14.7, 3.2 Hz, 1H), 2.53-2.48 (m, 2H), 2.14 (dd, J=14.6, 11.5 Hz, 1H), 2.05 (t, J=7.5 Hz, 2H), 1.86-1.75 (m, 5H), 1.74-1.65 (m, 4H), 1.61-1.49 (m, 4H), 1.45 (s, 3H), 1.38-1.33 (m, 2H), 1.22-1.20 (m, 2H), 1.13 (d, J=6.3 Hz, 3H), 1.10-1.00 (m, 2H), 0.90 (d, J=6.8 Hz, 3H), 0.85-0.71 (m, 4H).
ESI-MS, positive mode: m/z=1247.6 [M+Na]+.
HRMS (ESI) calcd for C72H89N8O10 [M+H]+ 1225.6696, found 1225.6689.
4-610CP-COOH (20)—TFA salt (10 mg, 0.0175 mmol, 1 eq.), DIPEA (15 μL, 0.0875 mmol, 5 eq.) and HBTU (8.0 mg, 0.021 mmol, 1.2 eq.) were dissolved in 400 μL of dry MeCN and stirred at room temperature for 5 min.
A solution of NH2—(CH2)2—O—(CH2)2—O—(CH2)6—Cl (6 mg, 0.0267 mmol, 1.53 eq.) in 100 μL of dry MeCN was added to the reaction mixture and stirring continued for 1 hour. Reaction mixture was neutralised with formic acid and product was purified by preparative HPLC (preparative column: Eurospher 100 C18, 5 μm, 250×20 mm; solvent A: acetonitrile, solvent B: H2O+0.2% v/v HCOOH; temperature 25° C., gradient A:B—5 min 40:60 isocratic, 5-30 min 30:70 to 100:0 gradient). Product was purified one more time by flash chromatography (silica-gel cartridge: Interchim Puriflash 12 g, 15 μm column, gradient 20% to 80% CH2Cl2—CH2Cl2:MeOH [90:10]). Solvents were removed and a product was lyophilised from acetonitrile: water mixture to obtain 7 mg (60%) of blue-violet powder.
1H NMR (400 MHz, d6-DMSO) δ 9.34 (t, J=5.5 Hz, 1H), 7.83 (dd, J=7.5, 1.0 Hz, 1H), 7.70 (t, J=7.6 Hz, 1H), 7.06 (dd, J=7.7, 1.0 Hz, 1H), 6.91 (d, J=2.2 Hz, 2H), 6.63-6.52 (m, 4H), 3.63 (t, J=5.9 Hz, 2H), 3.60-3.49 (m, 8H), 3.38 (t, J=6.5 Hz, 2H), 2.94 (s, 12H), 1.82 (s, 3H), 1.72 (s, 3H), 1.71-1.61 (m, 2H), 1.47 (q, J=6.9 Hz, 2H), 1.37-1.26 (m, 4H).
13C NMR (126 MHz, d6-DMSO) b 169.4, 164.7, 156.5, 150.6, 146.0, 135.0, 134.5, 129.3, 128.4, 125.2, 121.9, 118.1, 111.7, 109.1, 86.6, 70.2, 69.7, 69.5, 68.9, 45.4, 39.9, 39.8, 37.9, 34.6, 33.5, 32.0, 29.1, 26.1, 24.9.
ESI-MS, positive mode: m/z=662.3 [M+H]+.
The neighboring group effect (NGE) as described above could be detected by NMR or HPLC methods (
FIG. 1 shows the occurrence of neighboring group effect in the 4′-isomers of rhodamines. a, Neighboring group effect shifts spirolactone-zwitterion equilibrium of rhodamines. X═O, S, NR5, CR5R6, SO2, P(O)OH, P(O)OR5, SiR5R6, GeR5R6, Rn=alkyl. b, Chemical shift differences of amide proton of TMR-LTX regioisomeric probes. c, Comparison of retention times of TMR-LTX regioisomeric probes in HPLC analysis with SB-C18 column and isocratic elution conditions (75:25 MeOH:H2O 25 mM HCOONH4 pH=3.6).
The ability of the fluorophores to switch between spirolactone and zwitterion states can be characterized by measuring the absorbance in water-1,4-dioxane mixtures with known dielectric constant (Åkerlöf G, Short A O. J Am Chem Soc 1936, 58(7): 1241-1243). D50 is commonly used as numeric value for the evaluation of the equilibrium changes and represents a dielectric constant at which absorbance is halved.
a) shows graphs representing the absorbance of TMR-LTX positional isomers at λmax versus dielectric constant (D) of 1,4-dioxane-water mixtures and
As evident from
The following Table 1 shows comparative D50 values for different positional isomers of selected rhodamine probes.
The following Table 2 shows comparative chemical shift of amide NH proton in DMSO-d6 of isomeric dye-C8-taxane conjugates
As demonstrated in further experiments and
As mentioned above,
Furthermore, said increased cell permeability and favourable optical properties are also observed with multiple targeting moieties, as demonstrated with ligands targeting actin, DNA and Halo-tag (
The novel fluorescent probes were applied for STED nanoscopy imaging in living cells. For these experiments, STED optical microscopy was performed as indicated above.
The inventors obtained an unprecedented quality of images at resolution below diffraction limit. A combination of TMR and 610CP was successfully applied for two-color imaging of living cells.
| Number | Date | Country | Kind |
|---|---|---|---|
| 20156964.7 | Feb 2020 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2021/053319 | 2/11/2021 | WO |
