Claims
- 1. A method for detecting a thiol containing species in a sample comprising:
contacting a sample with a mercurimethyl-dihydropsoralen compound or a derivative thereof so that a reaction product of the mercurimethyl-dihydropsoralen or the derivative thereof and any thiol containing species in the sample is formed; and detecting any fluorescence in the sample, wherein fluorescence is indicative of a thiol containing species in the sample.
- 2. A method for fluorescently labeling a thiol containing species comprising reacting a thiol containing species with a mercurimethyl-dihydropsoralen compound or a derivative thereof.
- 3. A fluorescently labeled reaction product comprising a thiol containing species and a mercurimethyl-dihydropsoralen compound or derivative thereof covalently attached thereto.
- 4. The fluorescently labeled reaction product of claim 3 wherein the thiol containing species is an antibody or an enzyme.
- 5. A method for detecting nucleic acids in a sample comprising contacting a sample suspected of containing nucleic acid sequences with a fluorescently labeled reaction product comprising a thiol containing species and a mercurimethyl-dihydropsoralen compound or derivative thereof so that the fluorescently labeled reaction product intercalates into any nucleic acids in the sample, and detecting fluorescence, wherein fluorescence is indicative of a nucleic acid in the sample.
- 6. A method for fluorescently labeling a selected nucleic acid comprising contacting a selected nucleic acid with a fluorescently labeled reaction product comprising a thiol containing species and a mercurimethyl-dihydropsoralen compound or a derivative thereof so that the fluorescently labeled reaction product intercalates into the selected nucleic acid.
- 7. A fluorescently labeled nucleic acid comprising a selected nucleic acid intercalated with a fluorescently labeled reaction product comprising a thiol containing species and a mercurimethyl-dihydropsoralen compound or a derivative thereof.
- 8. A kit for fluorescently labeling or detecting thiol containing species comprising a mercurimethyl-dihydropsoralen compound or a derivative thereof.
- 9. A kit for fluorescently labeling or detecting nucleic acids comprising a fluorescently labeled reaction product of mercurimethyl-dihydropsoralen compound or a derivative thereof attached to a thiol containing species which intercalates nucleic acids.
Parent Case Info
[0001] This application claims the benefit of provisional U.S. application Ser. No. 60/370,951, filed Apr. 8, 2002, which is herein incorporated by reference in its entirety.
INTRODUCTION
[0002] This invention was supported in part by funds from the U.S. government (NIH Grant Nos. ES03647, ES06897 and ES05022) and the U.S. government may therefore have certain rights in the invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60370951 |
Apr 2002 |
US |