FLUORESCENT THIOL PROBES - CYTOMETRY AND PCR TECHNOLOGY

Information

• Research Project
• 3493108

• ApplicationId

  3493108

• Core Project Number

  R43CA057067

• Full Project Number

  1R43CA057067-01

• Serial Number

  57067

• FOA Number

• Sub Project Id

• Project Start Date

  6/2/1992 - 32 years ago

• Project End Date

  11/30/1992 - 31 years ago

• Program Officer Name

• Budget Start Date

  6/2/1992 - 32 years ago

• Budget End Date

  11/30/1992 - 31 years ago

• Fiscal Year

  1992

• Support Year

  1

• Suffix

• Award Notice Date

  6/2/1992 - 32 years ago

Organizations

• COVALENT ASSOCIATES, INC.

We propose to synthesize new probes for the determination of intracellular glutathione and other cellular thiols. The reaction product of the probe with thiols will have longer wavelength absorption and emission and higher quantum yield than the best cytosolic thiol fluorophore currently in use for cell sorting by flow cytometry. The dye will have the advantage of being cell membrane permeable so that cell loading can occur at lower extra cellular dye concentrations than currently possible. The synthesized probes will be tested in vitro and in cell cultures for absorption and emission properties when bound to glutathione and other protein thiols. Cell toxicity will also be determined.

IC Name

• Activity

  R43

• Administering IC

  CA

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  394

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  SSS

• Study Section Name

• Organization Name

  COVALENT ASSOCIATES, INC.

• Organization Department

• Organization DUNS

• Organization City

  WOBURN

• Organization State

  MA

• Organization Country

  UNITED STATES

• Organization Zip Code

  01801

• Organization District

  UNITED STATES