Patent Application
20220016174
The present invention relates to the application of regenerative medicine in livestock, especially with regenerative medicine for the care of cattle, and even more especially, with regenerative medicine in the care of milk-producing animals, including bovines. In particular, the invention relates to a composition of fetal mesenchymal stem cells (MSC) of allogeneic origin, treatment method and its use in mastitis in milk-producing animals, including bovines, suffering from mastitis, and to immunostimulate said animal, where MSCs come from fetal bovine adipose tissue. The composition can be administered intramammary, exerting an immunomodulatory effect on the inflammatory response and strengthening the antimicrobial activity against pathogens that cause mastitis in milk-producing animals with mastitis and becoming an alternative for the replacement and/or complementation of the use of antibiotics
The dairy industry is a business with high economic impact that delivers nutritional products to different age groups globally and enables the economic support of medium to small-scale producers in developing countries.
To a large extent, this industry relies on the production potential of the bovine mammary gland, which is capable of producing <8000 kg of milk per lactation. Inflammation of the mammary gland or mastitis, generally as a consequence of infectious agents, causes damage to the mammary tissue, reducing the number and activity of epithelial cells, consequently decreasing milk production. Mastitis is the disease with the highest prevalence and economic cost among dairy cattle worldwide. In studies carried out in farms in the central zone of Chile, prevalence of mastitis (clinical and subclinical) has been determined and varies between 65 and 88%. Meanwhile, an economic cost associated with mastitis for the dairy industry has been estimated at approximately USD $2 billion annually. In addition to the economic losses, mastitis has been declared as one of the main pathologies that threaten animal welfare. Bovine mastitis also affects public health by producing physical and usually microbiological changes in milk, as well as increasing the risk for the presence of residues.
Nowadays, despite the improvements that have been implemented in milking hygiene, as well as in the general management of dairy cattle, mastitis is still the most prevalent pathology that generates greater economic losses for both the producer and the dairy industry. The treatment and prevention of mastitis are complex because of bacterial multi-causality. In order to control the growth of the bacteria responsible for the infection of the mammary gland the main treatment and prevention strategy involves the use of antibiotics such as cephalosporins, penicillins, streptomycins and tetracyclines. However, this treatment is not entirely effective and its improper use has been associated to the increase in the resistance of pathogenic bacteria and a greater risk of antibiotics presence in milk. Consequently, recent evidence has emerged indicating that the use of antibiotics in agriculture would be responsible for the increase in bacterial resistance and would affect the efficacy of these antibiotics in humans.
The presence of these antibiotics in milk not only represents a penalty for the dairy producer but is also of great importance for public health and the safety of milk for human consumption. On the other hand, antibiotic treatment does not address the regeneration of mammary glandular tissue, the integrity of which is critical in terms of milk production.
Additionally, in recent years a series of vaccines have been developed to control bovine mastitis. These types of vaccines have mitigated the severity of the infection, however, they have not shown significant effects in preventing the presentation of new cases given a high variation in the response to immunization between animals.
Comparing antibiotics and vaccines, it is possible to indicate that the former have advantages including a high cost-benefit ratio and a high effectiveness in pathogens other than Staphylococcus aureus (S. aureus). Some disadvantages of antibiotics are a low effectiveness in mastitis caused by S. aureus, the potential risk of generating resistance from pathogens, generating residues in the milk, the requirement of a period of storage or lack of milk and the lack of a regenerative effect of the mammary gland. Meanwhile, vaccines have advantages including their low cost, the ability to reduce the severity of the condition and the associated costs of treatment; whereas, disadvantages include their low effectiveness in preventing the presentation of new cases and the presence of a maximum limit of protection.
Stem cells have been a focus of intense research during the last decades, changing the perspective of development, pathophysiology and treatment of various diseases (Joseph N M, Morrison S J. 2005. Toward an understanding of the physiological function of mammalian stem cells. Dev Cell 9, 173-183; Clarke M F, Fuller M. 2006. Stem cells and cancer: two faces of eve. Cell 124, 1111-1115). Stem cells are commonly defined as cells with unlimited capacity for self-renewal and differentiation towards all or some cell types of a tissue or organ, and are categorized into embryonic stem cells (ESC) and adult stem cells, among which, best known correspond to mesenchymal stem cells (MSCs). MSCs possess limited differentiation potential to mesenchymal (multipotent) cell lineages compared to ESCs that possess broader (pluripotent) differentiation potential (Jaenisch R, Young R. 2008. Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 132, 4, 567-582). The main function of adult stem cells is to form specialized cells in a living organism and to maintain and repair the tissue in which they are found. They are defined by their ability to self-renew, generate differentiated progeny, and express specific molecular markers (Caplan A I. 1991. Mesenchymal stem cells. J Orthop Res 9, 5, 641-650). The main in vivo function of bone marrow-derived MSCs is to enhance the formation of hematopoietic cells and contribute to bone repair (Pittinger et al., 1999). These cells represent a very low proportion of the bone marrow cell population, with values ranging from 0.001 to 0.01% (Pittenger M F, Mackay A M, Beck S C, Jaiswal R K, Douglas R, Mosca J D, Moorman M A, Simonetti D W, Craig S, Marshak Dr. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284, 541 1, 143-147). MSCs have been derived from different species mainly based on their ability to adhere to culture plates and the identification of their fibroblast morphology (Cortes Y, Ojeda M, Araya D, Dueñas F, Fernández M S, Peralta O A. 2013. Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res 9, 133, 1-14). Once isolated, MSC expansion requires a significant culture time ranging from 3 to 6 weeks. (Godwin E E, Young N J, Dudhia J, Beamish I C, Smith R K. 2012. Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon. Equine Vet J 44, 25-32). Subsequently, it is possible to evaluate their differentiation capacity through in vitro induction towards mesodermal lineages such as osteocytes, chondrocytes and adipocytes (Cortes Y, Ojeda M, Araya D, Dueñas F, Fernández M S, Peralta O A. 2013. Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res 9, 133, 1-14). The ability to adhere to plastic, the potential for mesodermal differentiation, the expression of mesenchymal markers (CD73, CD90 and CD105) and the absence of hematopoietic markers (CD45, CD34 and CD14), are the criteria established by the International Society for Cell Therapy for the definition of a human MSC population (Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F C, Krause D S, Deans R J, Keating A, Prockop D J, Horwitz E M: Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 2006, 8, 315-317). On the other hand, a MSC population has been described in adipose tissue that can be isolated by enzymatic digestion and subsequent filtration. The adherence to the plastic and its subsequent expansion allows obtaining relatively homogeneous MSC cultures. MSCs from adipose tissue express the major mouse and human class I histocompatibility complex but not class II. Yanez R, Lamana M L, García-Castro J, Colmenero I, Ramírez M, Bueren J A. Stem Cells 2006, 24, 2582-2591. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease. Stem Cells 24, 2582-2591). Additionally, MSCs derived from human adipose tissue do not stimulate the proliferation of lymphocytes, but rather inhibit the proliferation of T cells and stimulate peripheral mononuclear cells in the blood, which indicates that these cells would not cause an immune response if used in autologous therapy (Minteer D M, Marra K G, Rubín J P. 2015. Adipose stem cell: biology, safety, regulation and regenerative potential. Clin Plast Surg 42, 2, 169-179). Adipose tissue MSCs have been differentiated into osteogenic, adipogenic and chondrogenic lineages (Gronthos S, Franklin D M, Leddy F I A, Robey P G, Storms R W, Gimble J M. 2001. Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol 189, 54-63; Katz A J, Tholpady A, Tholpady S S, Shang H, Ogle R C. 2005. Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells. Stem Cells 23, 412-423; Zhu Y, Liu T, Song K, Ning R, Ma X, Cui Z. 2009. ADSCs differentiated into cardiomyocytes in cardiac microenvironment. Mol Cell Biochem 324, 1 17-129). In comparative studies carried out between MSC of bone marrow and adipose tissue, it has been reported that the latter are less efficient in terms of their capacity for osteogenic and chondrogenic differentiation, despite their great similarity in relation to the pattern of gene expression during the differentiation process. (Liu T M, Martina M, Hutmacher D W, Huí J H, Lee E H, Lim B. 2007. Identification of common pathways mediating differentiation of bone marrow- and adipose tissue-derived human mesenchymal stem cells into three mesenchymal lineages. Stem Cells 25, 750-760; Yoshimura H, Muneta T, Nimura A, Yokoyama A, Koga H, Sekiya I. 2007. Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res 327, 449-462). In bovine species, there are still no studies that allow determining differences between MSCs derived from bone marrow or adipose tissue.
Regarding the population of stem cells in the mammary gland, there is a large amount of information available in relation to progenitor cells of the mouse and human mammary gland, in studies that range from biological characterization to their possible role in cancer (Bruno R D, Smith G H. 201 1. Role of epithelial stem/progenitor cells in mammary cancer. Gene Expr 15, 133-140; VanKeymeulen et al., 201 1; Pond A C, Bin X, Batts T, Roarty K, Hilsenbeck S, Rosen J M. 2013. Fibroblast growth factor receptor signalling is essential for normal mammary gland development and stem cell function. Stem Cells 32, 178-189). In contrast, there is limited information regarding stem cells and their progeny in the bovine mammary gland. In this regard, the existence of a population of bovine mammary stem cells (MaSC) has been reported that would fulfil the function of generating common cellular progenitors with the capacity to differentiate into luminal and myoepithelial epithelial progenitor cells (Capuco A V, Choudhary R K, Daniels K M, Li R W, Evock-Clover C M. 2012. Bovine mammary stem cells: cell biology meets production agriculture. Animal 6, 382-393). This cellular hierarchy plays a fundamental role, especially in the postnatal development of the mammary gland, since it allows its growth from birth (Capuco A V, Akers R M, Smith J J. 1997. Mammary growth in Holstein cows during the dry period: quantification of nucleic acids and histology. J Dairy Sci 80, 477-487), the epithelial cells being important participants in the defense mechanisms against breast infection (Wellnitz O, Kerr D E. 2004. Cryopreserved bovine mammary cells to model epithelial response to infection. Vet Immunol Immunopathol 101, 191-202). Due to its regenerative potential, the use of MSC could have a strong and positive impact on the repair of damaged udder tissue, including epithelial, myoepithelial and stromal tissue. So far, there are two cell types, epithelial and myoepithelial progenitors, which are of considerable interest for their regeneration by therapeutic manipulation. Therefore, the expansion in the number of MSC through its pharmacological induction or its replacement by cell therapy with MSC has interesting potential applications both for the regeneration of damaged tissue, the increase in the milk production capacity and also its application in the production of proteins for pharmacological application in milk.
Regarding allogeneic therapies using fetal MSCs, it is important to note that most of the clinical studies reported in Veterinary Medicine are based on the use of MSC as autologous therapy (Webster R A, Blaber S P, Herbert B R, Wilkins M R, Vesey G 2012. The role of mesenchymal stem cells in veterinary therapeutics—a review. N Z Vet J 60, 5, 265-272). In this type of treatment, cells are collected and applied for treatment in the same donor. Autologous MSC collection is a relatively simple process, but requires specialized equipment and infrastructure, and isolation and expansion of cultures can take from days to weeks (Schnabel L V, Fortier L A, McIlwraith C W, Nobert K M. 2013. Therapeutic use of stem cells in horses: which type, how, and when? Vet J 197, 3, 570-577). This period of cell expansion can generate a delay in the application of the treatment (4 to 6 weeks) that in some cases is highly limiting for the success of the therapy. MSCs express low levels of major histocompatibility complex (MHC) I and II antigens and lack T-lymphocyte costimulatory molecules (CD80 and CD86) (Patel et al., 2008). These properties allow MSCs to be recognized as “immune-evasive” cells, being able to avoid their detection by the immune system and therefore serve for allogeneic therapy (Aggarwal S, Pittenger M F. 2005. Human mesenchymal stem cells modulate allogenic immune cell responses. Blood 105, 1815-1822). Therefore, obtaining MSC from donors can allow the generation of a cell stock of immediate bioavailability for use. The use of allogeneic sources makes it possible to have a bank or permanent storage of MSCs for its therapeutic use and therefore avoid the time spent on extraction, isolation and expansion of MSCs, which is required in autologous therapies. For this purpose, the development of cell cryopreservation banks is required to facilitate the transport and immediate availability of cells. On the other hand, the use of MSCs obtained from fetuses presents a series of advantages in relation to other sources of MSCs. The availability of MSCs decreases during the growth of individuals, so the possibility of having isolated MSC from adult individuals decreases and is diametrically less than the fetal stage. In humans, this decrease has been calculated to range from 0.001 to 0.0004% before age 30 to 0.00025 to 0.00005% after age 60 (Caplan A I. 2007. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol 213, 341-317). Additionally, MSCs isolated from adult individuals have lower differentiation potential compared to fetal MSCs (Zhang Z Y, Teoh S H, Chong M S, Schantz J T, Fisk N M, Choolani M A, Chan J. 2009. Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells, 27, 126-137). Furthermore, MSCs extracted from adult individuals have been more exposed to diseases in the donor that can affect their proliferative and differentiation potential. Fetal MSCs have also been described as having a lower immunogenic capacity compared to adult MSCs due to lower expression of noncytosolic MHC I and II (Le Blanc K, Tammik L, Sundberg B, Haynesworth S E, Ringdén O. 2003. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses in dependently of the major histocompatibility complex. Scand J Immunol 57, 1 1-20; Zhang Z Y, Teoh S H, Chong M S, Schantz J T, Fisk N M, Choolani M A, Chan J. 2009. Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells, 27, 126-137).
The properties of MSCs for the treatment of clinical disorders are known, and a large number of studies have reported the therapeutic application of MSC for the treatment of various clinical disorders, including myocardial infarction (Lee R H, Pulin A A, Seo M J, Kota D J, Ylostalo J, Larson B L, Semprun-Prieto L, Delafontaine P, Prockop D J. 2009. Intravenous HMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6. Cell Stem Cell 5, 54-63), diabetes (Lee R H, Seo M J, Reger R L, Spees J L, Pulin A A, Olson S D, Prockop D J. 2006. Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD/scid mice. Proc Nati Acad Sci USA 103, 17438-17443), sepsis (Nemeth K, Leelahavanichkul A, Yuen P S, Mayer B, Parmelee A, Doi K, Robey P G, Leelahavanichkul K, Koller B H, Brown J M, Hu X, Jelinek I, Star R A, Mezey E. 2009. Bone marrow stromal cells attenuate sepsis via prostaglandin e (2)-dependent reprogramming of host macrophages to increase their interleukin-10 production. Nat Med 15: 42-49), liver deficiency (Parekkadan B, van Poli D, Suganuma K, Carter E A, Berthiaume F, Tilles A W, Yarmush M L. 2007. Mesenchymal stem cell-derived molecules reverse fulminant hepatic failure. PLoS One 2, e941), acute renal failure (Togel F, Hu Z, Weiss K, Isaac J, Lange C, Westenfelder C. 2005. Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am J Physiol Renal Physiol, 289, F31-F42) and lung disease (Xu J, Qu J, Cao L, Sai Y, Chen C, Fie L, Yu L. 2008. Mesenchymal stem cell-based angiopoietin-1 gene therapy for acute lung injury induced by lipopolysaccharide in mice. J Pathol 214, 472-481). Several properties make the use of MSCs attractive for infectious pathologies. The ability to migrate to the sites of tissue damage, a property known as “homing”, is one of these properties (Rojas M, Xu J, Woods C R, Mora A L, Spears W, Román J, Brigham K L. 2005. Bone marrow-derived mesenchymal stem cells in repair of the injured lung. Am J Respir Cell Mol Biol. 33, 145-152). After their systemic or local administration, MSCs are attracted by chemotactic factors generated at the site of tissue damage (Kidd S, Spaeth E, Dembinski J L, Dietrich M, Watson K, Klopp A, Battula V L, Weil M, Andreeff M, Marini F C. 2009. Direct evidence of mesenchymal stem cell tropism for tumor and wounding microenvironments using in vivo bioluminescent imaging. Stem Cells 27, 2614-2623). MSCs express a series of receptors for some growth factors such as PDGF and IGF-1 and chemokines such as CCR2, CCR3, CCR4 and CCLS (Ponte A L, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J. 2007. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells 25, 1737-1745). The interaction between MSCs and endothelial cells of blood vessels would be mediated by several surface integrins such as Integrin a4/p1, which mediates cell-cell and cell-extracellular matrix interactions by adhering to the molecule (VCAN)-1 and the region V for fibronectin, respectively (Lewinsohn D M, Bargatze R F, Butcher E C. 1987. Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes. J Immunol 138, 4313-4321).
Additionally, MSCs have the capacity to modulate the immune response as has been reported in treatments carried out in patients with Crohn's disease (García-Olmo et al., 2005). Immunosuppressive function could be associated with the blocking of inflammatory molecules such as IL6, IL10, MMPs and nitric oxide (Jarvinen L, Badri L, Wettlaufer S, Ohtsuka T, Standiford T J, Toews G B, Pinsky D J, Peters-Golden, M, Lama V N 2008. Lung resident mesenchymal stem cells isolated from human lung allografts inhibit T cell proliferation via a soluble mediator. J Immunol 181, 4389-4396). Alternatively, MSCs can stimulate the immune response by producing pro-inflammatory cytokines (Rasmusson I, LeBlanc K, Sundberg B, Ringdén O. 2007. Mesenchymal stem cells stimulate antibody secretion in human B cells. Scand J Immunol 65, 336-343). This dual immunoregulatory function could be dependent on cell concentration (Le Blanc K, Tammik L, Sundberg B, Haynesworth S E, Ringdén O. 2003. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses in dependently of the major histocompatibility complex. Scand J Immunol 57, 1 1-20; Guest D J, Smith M R, Alien W R. 2008. Monitoring the fate of autologous and allogenic mesenchymal progenitor cells injected into the superficial digital flexor tendon of horses: preliminary study. Equine Vet 40, 178-181). Regarding regenerative capacity, MSCs have shown potential for multilineage differentiation in in vitro studies (Cortes Y, Ojeda M, Araya D, Dueñas F, Fernández M S, Peralta O A. 2013. Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res 9, 133, 1-14). The potential for transdifferentiation and implantation has been reported in vivo (Krause D S, Theise N D, Collector M I, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis S J. 2001. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 105, 369-377); however, its regenerative effect is currently considered to be based mainly on the secretion of paracrine factors that stimulate the activity of host cells (Fang X, Neyrinck A P, Matthay M A, Lee J W. 2010. Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type ii cells by secretion of angiopoietin-1. J Biol Chem 285, 2621 1-26222). Additionally, MSCs have been reported to have an antimicrobial potential mediated by paracrine factors such as keratinocyte growth factor (KGF), antimicrobial peptides, Angiopoietin 1 (ANG1), Interleukin 1 (IL1) and Prostaglandin E2 (PGE2), in addition to stimulation of macrophage and monocyte phagocytosis (Lee J W, Fang X, Krasnodembskaya A, Howard J P, Matthay M A. 201 1. Concise review: Mesenchymal stem cells for acute lung injury: role of paracrine soluble factors. Stem Cells 29, 6, 913-919). In general, terms, the properties of migration, immunomodulation and regeneration would be of substantial importance for its therapeutic application in lesions produced by infectious agents. In the case of mastitis, the intramammary administration of MSC would allow these cells to be distributed in the places of injury of the mammary gland where they would exert an immunomodulatory effect on the immune response, antimicrobial against the pathogen and subsequently regenerative of the damaged glandular tissue. This cell therapy could replace or complement the antimicrobial effect of antibiotics in mastitis, making it possible to avoid or reduce their use or alternatively to reinforce their effect.
It is known for bovine mastitis that S. aureus is one of its main causing pathogens and that it produces a number of virulence factors including hemolysin, coagulase, and leukocidin (Sutra L, Poutrel B. 1994. Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol 40, 79-89). Persistent infection with S. aureus has been associated with a deficient immune response as a consequence of virulence factors. Its intramammary infection generates a chronic condition with great potential for resistance to antibiotic therapy. In national studies, high resistance of S. aureus (24.7-38.9% of isolates resistant) to various antibiotics including Penicillin, Amoxicillin and Streptomycin has been reported (San Martin et al., 2002). In the southern zone of Chile, which comprises most of the national herds, contagious mastitis occurs more frequently, the main agent of which is S. aureus (San Martin et al., 2002). Inoculation of 900 colony-forming units (CFUs) of S. aureus through the nipple canal has been reported to induce clinical signs in each infected quarter and a peak in somatic cell count (SCC) of 1400×103 (Talbot B G, Lacasse P. 2005. Progress in the development of mastitis vaccines. Livestock Prod Sci 98, 101-1 13).
In particular, in the prior state of the art it is possible to mention the publication of the International Journal of Biological Sciences 2013; 9 (8): 818-829. doi: 10.7150/ijbs.6901 Research Paper Stem Cell Research: A Novel Boulevard towards Improved Bovine Mastitis Management Neelesh Sharma et al. Received 2013 Jun. 13; Accepted: 2013 Aug. 2; Published: 2013 Aug. 20, which refers to clinical mastitis and its effects on tissues, animal welfare and dairy productivity. It proposes the use of mammary epithelial cells MECs (inner layer or luminal epithelial cells) and their stem cells; and an outer layer of myoepithelial cells. The inner layer of bovine MECs produces CK18 and CK19 and the outer layer CK14, a-SMA and p63. It indicates that the results obtained in humans and mice are encourage by the application of stem cells in bovines. Thus, he proposes the use of stem cells to correct the structural and cytological defects caused by mastitis in the udder of bovines. It reviews several types of stem cells that would have therapeutic utility and their potential use in therapies to manage post-mastitis damage in bovines and thus restore milk production. It is argue that the possibility of bovine mammary stem cell therapy would allow significant tissue regeneration and replace or repair diseased or damaged tissue by differentiation into epithelial, myoepithelial and/or cuboidal/columnar cells in the udder, with minimal risk of rejection and collateral effects.
Fetal Stem Cells in Farm Animals: Applications in Health and Production P S Yadav⋅R K Singh⋅B. Singh Received: 15 Oct. 2011/Accepted: 5 Dec. 201 1/Published online: 19 Jan. 2012 NAAS (National Academy of Agricultural Sciences) 2012 (Agrie Res (January-March 2012) 1 (1): 67-77 DOI 10.1007/s40003-01 1-0001-7) refers to scientifically validated embryonic stem cell lines (ES) in species other than mice, humans or some primates. It performs a review of the biological properties and easy derivation of fetal or adult stem cells that play a vital role in the health and production of livestock. They note that the placenta and fetal adnexa such as umbilical cord blood, umbilical cord matrix, fetal fibroblasts, and amniotic cells are an immensely valuable source of progenitor and pluripotent cell lineages. A large number of fetal cells are accessible, which can have various applications including assisted reproduction, regenerative medicine, or induced pluripotent stem cell production. It indicates that since fetal stem cells have markers of pluripotency expression and growth kinetics similar to ES cells, it is possible to think that they may be homologous to ES cells.
US 20140134140 A1 describes a method based on the use of human MSCs for the treatment of cystic fibrosis challenged with bacteria such as Pseudomona aeruginosa, Staphylococcus and Streptococcus pneumoniae.
The United States Food and Drug Administration (FDA) has approved the product “Hemacord” as a stem cell-based therapy in patients suffering from disorders of the hematopoietic system. The FDA has also approved for clinical phase I research, an MSC neural progenitor-based therapy for the treatment of multiple sclerosis in humans. The FDA also approved the production of placental-derived stem cells for the production of the product “Pluristem Placental Expanded”, with potential applications for the treatment of aplastic anemia and muscle injuries. The Canadian Food and Drug Administration has approved the PROCHYMAL product for stem cell-based therapy in immune rejection diseases in children suffering from post-bone marrow transplant complications in graft versus host disease (GVHD).
The present invention carried out a series of in vitro studies with MSCs isolated from fetal bovine bone marrow to induce osteogenic, chondrogenic and adipogenic differentiation of these cells (Cortes Y, Ojeda M, Araya D, Dueñas F, Fernández M S, Peralta O A. 2013. Isolation and multilineage differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res 9, 133, 1-14), demonstrating the ability of MSCs to differentiate into hepatogenic and neurogenic lineages (Dueñas F, Becerra V, Cortes Y, Vidal S, Sáenz L, Palomino J, De los Reyes M, Peralta O A. 2014. Hepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived bovine fetuses. BMC Vet Res 10, 154, 1-13), and the expression potential of the GFP reporter gene in bovine MSCs, as well as the expression profile of epigenetic regulation enzymes (Diaz et al., 2015).
The present invention then relates to mesenchymal stem cells (MSCs) from fetal bovine bone marrow—an abundant source of MSCs, possessing broad differentiation potential and enabling the preparation of an allogeneic cell therapy composition—that is, of different individuals, and provide a method for therapy and its use, in the treatment of bovine mastitis.
The present invention provides experimental data for a method of treatment and use of fetal MSC of allogeneic origin for mastitis in milk producing animals. The composition can be administered intramammary, exerting an immunomodulatory effect on the inflammatory host response, strengthening the antimicrobial activity against the pathogens that cause mastitis and promoting the regeneration of the mammary glandular tissue, shortening the recovery time in dairy cows with mastitis and becoming an alternative for the replacement and/or complementation for the use of antibiotics.
An objective of the present invention is a composition of fetal mesenchymal stem cells (MSCs) of allogeneic origin for the treatment of mastitis in dairy animals, including bovines, and for immunostimulating said animal suffering from mastitis. In particular, MSCs come from fetal bovine bone marrow. The composition can be administered intramammary, exerting an immunomodulatory effect on the inflammatory response and strengthening the antimicrobial activity against pathogens that cause mastitis in milk-producing animals with mastitis and becoming an alternative for the replacement and/or supplementation for the use of antibiotics.
The present composition could be used in combination with an antibiotic selected from cephalosporins, penicillins, streptomycins, tetracyclines, or a combination thereof.
Another objective of the present invention is a method for treating mastitis in milk-producing animals, including bovines, and for immunostimulating said animal suffering from mastitis, which comprises administering fetal mesenchymal stem cells (MSCs) of allogeneic origin, said MSCs preferably coming from of fetal bovine adipose tissue, and preferably, said MSCs are administered intramammary.
It is still another objective for the present invention, the use of fetal mesenchymal stem cells (MSCs) of allogeneic origin in the treatment of mastitis in dairy animals, including bovines, and to immunostimulate said animal suffering from mastitis, said MSCs preferably coming from fetal bovine adipose tissue, and preferably, said MSCs are administered intramammary.
It is still another objective of the present invention, the use of fetal mesenchymal stem cells (MSC) of allogeneic origin in the preparation of a veterinary drug useful in the treatment of mastitis in dairy animals, including cattle, and to immunostimulate said animal that suffers from mastitis, said MSCs preferably coming from fetal bovine adipose tissue, and preferably, said MSCs are administered intramammary.
The present composition comprises a suspension of fetal bovine MSC of allogeneic origin that can be administered intramammary, and useful as an alternative or complementary treatment method to the use of antibiotics. The composition and form of intramammary administration allows the distribution of these cells in the mammary tissue, exerting an immunomodulatory effect on the inflammatory response, controlling the infectious process, and therefore, shortening the recovery time in dairy cows with induced mastitis.
The present composition comprises a suspension of fetal bovine MSCs of allogeneic origin that can be administered intramammary; alternative or complementary treatment and use method to the use of antibiotics. The intramammary administration composition allows the distribution of these cells in the mammary tissue, exerting an immunomodulatory effect on the inflammatory response, controlling the infectious process, and therefore, shortening the recovery time in dairy cows with induced mastitis.
The present composition can be used in combination with an antibiotic selected from cephalosporins, penicillins, streptomycins, tetracyclines, or a combination thereof.
The present composition comprises fetal mesenchymal stem cells (MSCs) of allogeneic origin and serves in the treatment of mastitis in milk-producing animals, including bovines. The present composition is a substitute or complementary alternative to the use of antibiotics, increasing the effectiveness of the treatment and reducing the risk that its excessive use represents for public health.
The present regenerative mammary gland composition comprises allogeneic MSCs that are prepared by the following steps (
Cryopreservation and commercialization of MSCs (3), the wide availability of tissues and therefore derived cells, allows the development of a pool of MSCs of allogeneic origin for cryopreservation with abundant reserves for their use and commercialization. Application of an intramammary treatment method for allogeneic fetal MSCs for mastitis (4 and 5). Consequently, there is a decrease in the use of antibiotics in order to treat mastitis (6).
To determine the in vivo effects of MSCs—which should be dependent on the population of stem cell origin used, BM-MSCs and AT-MSCs were compared in terms of proliferation, immunomodulation and lymphocyte activation potentials. The in vivo effects of MSCs were also dependent on the tissue into which they were inoculated. The potential for migration (“homing”) and implantation (“engraftment”) to the target tissue was evaluated by identifying fluorescently labeled MSCs in the parenchyma of the mammary gland.
The present invention relates to a suspension of mesenchymal stem cells (MSC) of fetal and allogeneic origin for the treatment of mastitis in dairy animals, including bovines. The ability to migrate to the site of injury allows the treatment of mastitis, strengthening the immune response, exerting antimicrobial action and subsequently promoting the regeneration of damaged glandular tissue. The present composition also relates to a composition comprising such a suspension of allogeneic bovine MSCs derived from fetal tissues. This product can be stored by cryospreservation and can be administered intramammary.
The present invention would be a substitute or complementary alternative for the use of antibiotics, increasing the effectiveness of the treatment and reducing the risk that their excessive use represents for public health. Currently, there is no such tool available for the treatment of mastitis in dairy animals, including cattle, thus generating great therapeutic, productive and animal welfare benefits with the present invention.
Trials were conducted to evaluate efficacy parameters of intramammary administration of the allogeneic composition of fetal bovine MSC in cows with induced mastitis.
In the examples below—unless otherwise stated, trials were conducted using 15 clinically healthy (free from Brucellosis, Tuberculosis and Leukosis) Holstein Friesian cows of approximately 2 years of age. Three bacterial milk cultures were performed to rule out the presence of intramammary infections. Cows were milked twice daily using a separate milking unit and milk production was quantified after each milking. The trial was conducted according to the Applicant's Bioethics guidelines. The animals were kept under the same management and were fed alfalfa and concentrate.
A strain of S. aureus was thawed and seeded on blood agar and incubated at 37° C. in aerobiosis overnight. A bacterial colony of S. aureus was then incubated in 5 mL of brain heart broth in a 15 mL tube for 10 hours at 37° C. Subsequently, eight dilutions of the broth were prepared in PBS and 100 uL of each dilution were seeded in duplicate blood agar. Using a sterile rake, the inoculum of each dilution was distributed on the surface of each plate. The plates were incubated overnight in aerobiosis at 37° C. Subsequently, the colonies on the plate containing between 30-300 CFU were counted in duplicate and averages were obtained. To calculate the number of CFU per mL, the equation (Eq. 1) was used:
Number of colonies×(10)A(number of the dilution+1)=Amount of CFU/mL (Eq. 1)
The volume necessary to obtain a bacterial suspension with a concentration of 1000 CFU/mL in 1 Lt of PBS was calculated using the equation (Eq. 2):
C1×V1=C2×V2 (Eq. 2)
where C1=Concentration expressed in CFU/mL, V1=Volume to be extracted from the incubated broth, C2=Desired concentration (1000 CFU/mL) and V2=Total volume of the suspension for inoculate (1000 mL).
The cows (n=15) at approximately 30 days postpartum were challenged by inoculation of the two left mammary quarters via the intracisternal route administration of 1 mL of a 1000 cfu/mL suspension of S. aureus. 1 mL of PBS was inoculated into the right quarters as a control.
From the day of the challenge and daily for 15 days, the cows were clinically evaluated and milk samples were collected to determine SCC and CFU. The clinical evaluation and the collection of milk samples were carried out as detailed below.
Experimental Design
The cows were randomly assigned to three experimental groups of 5 animals each. All the animals were challenged by inoculation of the two left mammary quarters via the intracisternal route using 1 mL of a 1000 CFU/mL suspension of S. aureus.
1 mL of PBS was inoculated into the right quarters as a control. The NEG group (without therapy) was treated on days 4 and 10 with a 5 mL Ringer Lactate solution via the teat canal in the two infected quarters. The ATB group (antibiotic therapy) was treated with a commercial suspension of 50 mg of pirlimycin on experimental days 4 and 5 via the teat canal in the two infected quarters. The MSC group (MSC therapy) was administered with a 2.5×107 suspension of MSC in 3 ml of Lactated Ringer via the teat canal in the two infected quarters. From the day of the challenge and daily during 15 days, the cows were clinically evaluated and milk samples were collected daily to determine SCC and CFU (
Clinical Examination
From the day of the challenge and daily during 15 days, the cows were evaluated by clinical examination that included determination of heart rate, respiratory rate, ruminal rate, rectal temperature, and mucous coloration. According to the classification table provided below (Table 1.; Wenz J, Garry F, Barrington G. 2006. Comparison of disease severity scoring systems for dairy cattle with acute coliform mastitis. JAVMA. 229, 259-262). From two days prior to the challenge and daily, the udder of each cow was evaluated after each milking by manual palpation of each mammary quarter to detect abnormalities such as volume increase, pain, redness, and temperature increase (Table 1). The abnormalities in the appearance of the milk were evaluated at the beginning of milking using a strip cup with dark surface to classify the secretion as normal, watery, viscous, with the presence of lumps, blood or pus (
Bacteriological Analysis (CFU)
Milk samples prior to milking were collected aseptically from all cows following the protocols of the International Dairy Federation (1985) on three consecutive days prior to the experimental challenge (
Somatic Cell Count (SCC)
From two days prior to the challenge and daily for the 15 days of the study, milk samples were collected prior to milking from all cows in 50 ml plastic tubes with bronopol as preservative. SCC was performed on the milk samples using a Fossomatic flow cytometer (
Obtaining and Cultivating MSC
MSCs were isolated from bovine fetal adipose tissue. Bovine fetuses at the last third of gestation were obtained from an abattoir and transported in a thermal container to the laboratory. Fetal adipose tissue was extracted by incision in the abdominal midline and surgical extraction using a scalpel and sterile forceps. Then the adipose tissue was washed with phosphate buffer solution (PBS), pH 7.4 and sectioned using a scalpel. Subsequently, the tissue was incubated at 38° C. for 45 min under constant agitation in a digestion medium consisting of 0.5% collagenase diluted in HBSS solution (Hank's Balanced Salt Solution). The enzymatic reaction was stopped by adding expansion medium consisting of DMEM medium (Dulbecco's Modified Eagle's Medium) supplemented with 10% fetal bovine serum, 500 uL of amphotericin B and 500 uL of penicillin/streptomycin in an amount equivalent to the digestion medium. Subsequently, the tissue was filtered using pores of 40 um and centrifuged twice at 400×g for 5 min using washing medium, which consisted of DMEM supplemented with 100 ug/mL of amphoterecin B, 100 IU/mL of penicillin and 100 ug/mL streptomycin. The pellet was resuspended in expansion medium, and then transferred to 175 cm2 cell culture flasks. MSC cultures derived from adipose tissue were incubated at 38° C. under a humid atmosphere with 5% CO2. After 48 hours, the cells not adherent to the plastic were removed by changing the culture medium. Upon reaching 80 to 90% confluence, MSC passages were performed by removal with trypsin/EDTA, supplemented with 250 u·g/mL of amphoterecin B, 100 IU/mL of penicillin and 100 u·g/mL of streptomycin.
Statistical Analysis
The data on clinical variables, SSC and CFU were analysed using the Shapiro Wilks normality test. Treatment effects were evaluated by Kruskal Wallis analysis. The experimental days and the treatment were evaluated as independent variables, and the clinical variables, the SCR and the CFU were evaluated as dependent variables. A significance value (P<0.05) was used. All statistical analyzes were performed using the Info Stat software (Cordoba, Argentina, 2008).
Comparative Analysis of Clinical Variables in Cows Challenged with S. aureus
The cows showed an increase in volume of the challenged quarters from day 2 post-challenge. No traces of blood or pus were observed in the milk by strip cup with dark surface testing but lumps were detected in samples from all cows intermittently during the study. The average values of the clinical variables evaluated including heart rate, respiratory rate, ruminal rate, rectal temperature, thermography of the mammary quarters, and milk production did not show differences (P>0.05) between the experimental groups (Table 3). The average daily milk production of the NEG, ATB and MSC groups was 17.3±1.8; 16.7±0.8 and 14.7±1 I, respectively. There was a decrease in milk production between days 5 and 7 of the study of 6.8; 2.1 and 2.3 (NEG, ATB and MSC Groups, respectively).
38 ± 0.7
34 ± 0.6
34 ± 0.4
Comparative Analysis of RCS in Cows Challenged with S. aureus
From the day of the challenge and daily for 15 days, milk samples were collected to determine SCC. The SCC values were subsequently expressed as a linear score (LS-SCC) by means of logarithmic transformation (LS=LOGN). The mammary quarters of the NEG and ATB groups (without therapy and antibiotic therapy, respectively) obtained statistically similar LS-SCC values compared to the uninfected quarters from day 1 to day 10 post-challenge (
Comparative Analysis of CFU in Cows Challenged with S. aureus
From the day of the challenge and daily for 15 days, milk samples were collected for determination of CFU. The CFU values were subsequently expressed as a linear score (LS-CFU) by means of logarithmic transformation (LS=LOGN). Milk samples from the MSC group reached higher (P<0.05) PL-CFU (4.6) values compared with the samples obtained from the ATB group quarters (−0.7) on day 3 post-challenge (
The previous example demonstrates that the cows challenged with S. aureus and assigned to the MSC group reached the highest values of SCC and CFU (Days 2 and 3, respectively), after the challenge with S. aureus but prior to the administration of MSC. However, the group of cows treated with MSC significantly decreased SCC values on day 10 post-challenge. Furthermore, the group of cows treated with MSC reached intermediate values of CFU without showing significant differences compared to the groups without therapy and with antibiotic therapy. These data confirm that MSCs exerted an immunomodulatory and antibacterial effect in cows challenged with S. aureus.
Strains ATCC 25923 and SAU-1 S of S. aureus were used in order to determine their survival in unconcentrated MSC conditioned medium (CM), concentrated MSC conditioned medium (CCM), MSC conditioned medium activated by pre-exposure of S. aureus (ACM). In these same MSC cultures, the mRNA levels of the antimicrobial peptides b-defensin 4A (bBD4A) (
The CM were obtained from BM-MSCs, AT-MSCs and FBs cultures as control. Cells were seeded at a concentration of 6×103 cells/cm2 until reaching 70 to 80% of confluency. Subsequently, MSCs and FBs were cultured in DMEM medium with 4500 mg/L D-Glucose (Mediatech, Incorporated) without serum, antibiotic and antifungal for 72 h at 38° C. under a humid atmosphere with 5% CO2. Next, the CM were collected and then centrifuged at 400×g for 5 min and later filtered through pores of 0.22 μm and frozen at −20° C. until use. For the experiments with concentrated CM, the medium was concentrated 10 times its initial volume, by ultrafiltration using Amicon Ultra filters (Merck Millipore, Corporation, Cork, Ireland) with a 3 kDa NMWL membrane according to the manufacturer's instructions. The concentrated media were then frozen at −20° C. until used. Subsequently, the antibacterial potential of the activated CM obtained from cultures of BM-MSC, AT-MSCs, as well as FBs that were previously exposed to 300 CFU of the local strain SAU-1 S of S. aureus by a 6 h period (
Consequently, the above tests indicate that BM-MSCs and AT-MSC CM possess antibacterial effect against S. aureus in an in vitro culture system. This antibacterial potential is increased by concentrating the media by filtration and activating the CM by pre-exposure of MSCs to S. aureus. The results suggest that the antibacterial effect of MSCs is mediated by the expression of antimicrobial peptides NK1 and bBD4A. See
Isolation of MSC from fetal bone marrow (BM-MSCs). Bone marrow was obtained from femurs of bovine fetuses of 8 to 9 months of gestation (n=5) derived from slaughterhouse (
The suspension obtained was centrifuged at 400 G for 10 minutes at RT. Subsequently, the BM-MSC-MOs and AT-MSCs were seeded separately in expansion medium consisting of DMEM supplemented with 5% fetal bovine serum (FBS). Upon reaching 80 to 90% cell confluence, passages were made by stirring with 0.25% Trypsin/EDTA for 5 min. The MSCs were cultivated in expansion medium until reaching 80% confluence.
The proliferation potentials of bovine BM-MSCs and AT-MSC were compared by calculating population doubling (DP) according to tissue of origin. Population doublings were determined. BM-MSCs and AT-MSCs were seeded separately in triplicate in 3.5 cm culture plates in expansion medium until reaching 70 to 80% confluence. Then the MSCs were passed at a concentration of 8.4×103 cells/cm2. Cells from passages 1 to 15 were maintained with a fixed subculture period of 5 days and counted and reseeded. The cell count data were used to estimate the population doubling and to construct an accumulated population doubling by means of the equation (Eq. 1):
DP=ln(Nf/Ns) (Eq. 1)
Where ln is the natural logarithm, Nf is the number of cells obtained in the subculture and Ns is the number of cells initially seeded (Freshney R I. 2005. Culture of Animal Cells: A Manual of Basic Technique. 5th edition. Editorial Wiley-Liss. New Jersey. 38-358). To establish a proliferation curve with the total cumulative number of cells during the passages, the equation (Eq. 2) was used:
Number of total cells of Pn=Number counted in Pn×(Number counted at Pn-i/Total number of cells seeded) (Eq. 2)
The proliferation potential was determined at two concentrations (5000 and 8500 cells/cm2) for 15 days (
The proliferation and immunogenicity potentials of MSCs are dependent on the source of the tissue of origin, since MSCs derived from bone marrow possess high proliferation and immunogenicity potentials compared to MSCs derived from adipose tissue. BM-MSCs have greater proliferation potential, due to the fact that they double their population in less time compared to AT-MSCs from bovine fetuses. However, both BM-MSCs and AT-MSCs possess similar immunomodulation potentials.
mRNA levels of paracrine immunomodulatory factors in BM-MSCs and AT-MSCs preactivated with IFNγ. The immunomodulatory potential was evaluated by quantifying the expression of anti-inflammatory genes (IDO, IL-4, IL-10) in BM-MSCs and AT-MSCs treated with 10, 20 or 40 ng/mL of IFNγ. (
The Q-PCR data obtained were analyzed using the comparative method of Ct, in relation to the Ct of the endogenous genes GAPDH and p-ACTIN (Schmittgen T D, Livak K J. 2008. Analyzing real-time PCR data by the comparative C (T) method. Nat Protoc 3, 1 101-1 108). Determination of the enzymatic activity of IDO in conditioned medium of MSCs preactivated with IFNγ. BM-MSCs and AT-MSCs from 2nd to 3rd passage were seeded at a concentration of 1×104 cells/cm2 in high glucose DMEM medium supplemented with 10, 20 and 40 ng/mL of IFNγ (R&D Systems, Minneapolis, USA) for 72 h. Subsequently, the conditioned medium of MSC was collected to determine the enzymatic activity of IDO. For this purpose, the N-formyl-kinurenine concentration in the medium was quantified using a colorimetric assay (Saulnier N, Loriau J, Febre M, Robert C, Rakic R, Bonte T, Buff S, Maddens S. 2016. Canine placenta: A promising potential source of highly proliferative and immunomodulatory mesenchymal stromal cells? Vet Immunol Immunopathol 171, 47-55) and comparing it with a D-Quinurenine concentration curve (Sigma-Aldrich, USA). Once collected, the conditioned medium was centrifuged at 750 g for 5 min and filtered through pores of 0.22 μm. A 200 μl aliquot of MSC conditioned medium was mixed with 100 ml of 30% trichloroacetic acid (Sigma-Aldrich, USA) and the mixture was incubated at 50° C. for 30 minutes. Once centrifuged at 15,000 g for 1 min, 100 μl of supernatant was transferred to each well of a 96-well flat-bottom plate and 100 μl of Ehrlich reagent (100 mg of p-dimethylamino benzaldehyde diluted in 5 ml of acid glacial acetic). Next, the optical density at 450 nm was determined with a microplate reader (Model 3550, Bio-Rad, Arizona, USA). See
BM-MSCs and AT-MSCs treated with 20 and 40 ng/mL of IFNγ expressed (4.1, 1, 6 and 2.5, 1.4 times, respectively) higher (P<0.05) levels of mRNA of IL-6 in relation to the control MSC treated with 10 ng/mL of IFNγ (
Additionally, the expression levels of major histocompatibility complex immunogenicity genes I and II (MHC-I and II) and T lymphocyte costimulatory molecules (CD80 and CD86) were quantified (
These results indicate that both MSC cell types express immune mediating factors that could exert an immunomodulatory role for therapeutic treatment in cattle. Considering these results, it is possible to conclude that the immunogenicity potential of MSCs is dependent on the source of tissue origin, since BM-MSCs present a higher expression of MHC-I I compared to AT-MSCs, which could determine a higher Immune rejection in a potential application in allogeneic therapy.
The effect of conditioned medium of BM-MSCs and AT-MSCs on the proliferation of T lymphocytes activated by alloantigens in vitro was evaluated by means of mixed leukocyte reaction (MLR) (Bocharov G, Luzyanina T, Cupovic J, Ludewig B. 2013. Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis. Frontiers in immunology 264, 1-7). Bovine peripheral blood mononuclear cells (PBMC) were isolated by density gradient (Flistopaque-1077, Sigma-Aldrich). A 15-mL sample of bovine blood was drawn through the coccygeal vein using heparin vacutainer tubes. Then the blood was mixed with PBS in equal parts (1:1). On the other hand, in a 50 mL falcon tube, 15 mL of Histopaque (Sigma-Aldrich) were added and the blood solution with PBS (30 mL) was added without mixing. Subsequently, the tube was centrifuged at 900 g without brake for 35 minutes. In this way, a separation into four phases was achieved, plasma plus PBS, PBMCs, Histopaque and in the background granulocytes and red blood cells. With a pipet, the PBMC phase was extracted, then it was washed with 10 mL of PBS and centrifuged twice at 1000 g for 10 minutes, to then be re-suspended in supplemented RPMI. In order to perform the purification of peripheral blood lymphocytes (PBLs), the PBMCs were re-suspended in RPMI 1640 medium with 10% FBS (Hyclone Laboratories), 100 μg/mL of amphotericin B, 100 μg/mL of streptomycin and 100 IU/mL of penicillin in a 6-well plate (Costar 3516, USA) and incubated at 38° C., with 5% CO2 for 2 h. After that, the cells not adherent to the plastic were removed and washed with PBS. The PBL was centrifuged and the pellet was resuspended in PBS (Quah et al., 2007). Previously, cell counting was performed in a Neubauer chamber using trypan blue to determine cell viability. Subsequently, the cell concentration was adjusted to 2×106 cells per mL. The PBLs was subsequently labelled with a fluorescent carboxyfluorescein succinimidyl ester (CFSE) probe using a cell proliferation kit (CellTrace, Thermo Fisher, California, USA). Each vial of stock solution (5 mM) was diluted with 18 pL of DMSO and from this dilution 2 μL (10 pM) were used to mark a total of 1×106 PBLs in 1 mL of PBS in a suspension that was incubated for 20 min at 37° C. by gentle shaking, protected from light (Quah et al., 2007; Bocharov G, Luzyanina T, Cupovic J, Ludewig B. 2013. Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis. Frontiers in immunology 264, 1-7). Subsequently to this solution a five-fold proportion of medium with SFB was added and it was incubated again for 5 minutes, this process is carried out to eliminate the free dye that is in the solution. It was then centrifuged and the pellet was resuspended in culture medium for subsequent tests. The PBMCs was treated with mitomycin C to inhibit DNA synthesis, suppressing its proliferation but keeping the cells viable and capable of stimulating the proliferation of PBLs. For this, 1×106 PBMCs were previously treated with 50 μg/mL of mitomycin C at 37° C. for 2 h and then they were washed three times with PBS with 5% FBS. To induce alloproliferation, PBLs labeled with CFSE (CellTrace) as responder were co-incubated with PBMCs as allogeneic stimulator treated with mitomycin C in a 96-well U-bottom culture plate for 5 days (Bocharov G, Luzyanina T, Cupovic J, Ludewig B. 2013. Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis. Frontiers in immunology 264, 1-7). Different stimulator and responder ratios (1:1, 1:2 and 1:4) were incubated with the aim of standardizing the method for cattle and working with the proportion that stimulates the proliferation of PBLs to the highest level. As a proliferation control, PBMCs plus PBLs without conditioned medium was used. The cells were subsequently recovered for proliferation analysis by flow cytometry. The data were analyzed using a Gallios cytometer kit (Beckman Coulter, Brea, Calif., USA) and the Cell Quest program. See
The “scratch” migration test was used to determine the migratory capacity of MSCs, using fetal FBs as biological controls (
Transwell migration analysis was used to determine the potential for cell migration in response to chemotactic stromal cell-derived factor-1 (SDF-1) (
The potential between BM-MSCs and AT-MSCs was similar, which can be associated to similar expression levels of the CXCR4 receptor. The angiogenic potential of the MSC and FB conditioned medium was evaluated using the in vitro endothelial cell tubule formation assay (
Once they reached 80% confluence, the culture medium was removed and DMEM without SFB was added in order to produce conditioned medium. Once collected, the conditioned medium was concentrated 10 times by centrifugation for 30 minutes at 1800 rpm in Amicon Ultra Centrifugal filters (Merck Millipore, Tullagreen) and was subsequently frozen at −20° C. until use. For the tubule formation assay, 100 ml of matrigel without growth factors (Corning, Bedford) was pipetted into 24-well plates for the establishment of a basement membrane matrix. The matrigel was gelled at 38° C. for 30 minutes. Subsequently, the endothelial cells (1.8×105 cells/well) previously extracted were seeded and cultured on top of the gelled Matrigel membrane. These cells were then resuspended in the concentrated medium of BM-MSCs, AT-MSCs and FBs and seeded on the Matrigel layer. After 6 hours of incubation at 38° C., the tubules formed were quantified by obtaining digital images obtained with 4× magnification and evaluation using Image J software (NIMH). The concentrated AT-MSC conditioned medium induced greater (P<0.05) tubule formation, compared to BM-MSCs, FBs and their DMEM controls with 5% SFB and DMEM (
A safety test was carried out in healthy cows in order to determine the effect of inoculation, via the teat canal, of an MSC suspension of allogeneic origin on the clinical parameters including hemogram and biochemical profile in Holstein Friesian cows. MSCs were isolated from adipose tissue collected from abattoir-derived fetuses previously tested for Brucella abortus (Rose Bengal), Leptospirosis (Microagglutination), Bovine Viral Diarrhea (Elisa) and Infectious Bovine Rhinotracheitis (Elisa). Only fetuses negative for these diseases were used as donors (n=14). The safety test was carried out in a dairy farm of 997 lactating cows that has been declared free of Brucellosis, Tuberculosis and Bovine Leukosis. Clinically healthy Holstein Friesian heifers (n=8) of age (15.4±1.9 months) with weight (445.6±37.3 kg) were used. A total of 25×106 MSCs were suspended in 3,000 Ringer Lactate and this suspension was inoculated into each of two mammary quarters of each cow. A control suspension of 3 mL of Lactated Ringer was inoculated in each of the two remaining mammary quarters of each cow. The order of administration per room was determined randomly. Two doses of MSCs were administered 10 days apart (dose 1=day 0 and dose 2=day 10) and clinical parameters (heart rate, respiratory rate, ruminal rate, rectal temperature and mammary gland temperature) were evaluated daily from the day 0 to day 20 (Table 4).
In addition, blood samples were obtained from the coccygeal vein every 5 days from day 0 to day 20, for hemogram analysis and biochemical profile. Additionally, lymphocyte and monocyte populations were isolated from peripheral blood for analysis of CD3, CD4, CD8, and FoxP3 markers by quantitative PCR.
10 ± 0.3
The analysis of the data indicates that the administration of a 25×106 suspension of allogeneic MSCs via the teat canal, in two doses separated by ten days, does not significantly affect the clinical parameters, hemogram variables and biochemical profile and expression of markers. of CD4+ and CD8+ lymphocyte populations or on the mRNAs of the CD25 and CD62L lymphocyte activation molecules (
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CL2018/050046 | 6/15/2018 | WO | 00 |
