Claims
- 1. In a radioassay kit for radioassaying a specimen for folic acid, its metabolites or derivatives which bind to .beta.-lactoglobin and contains vials of:
- (a) N.sup.5 -methyltetrahydrofolic acid as a standard;
- (b) lysine buffer;
- (c) bovine serum albumin as a standard diluent;
- (d) .beta.-lactoglobin;
- (e) isotope diluent;
- (f) dextran; and
- (g) charcoal powder,
- the improvement which comprises the addition in a vial a chemical compound of the formula: ##STR4## wherein n is a positive integer of from 1 to 3 and R is radioactive iodine: I.sup.125 or I.sup.131 ; to said kit.
- 2. The radioassay kit of claim 1 in which n is 1 and R is I.sup.125.
- 3. In a method of radioassaying a specimen for folic acid, its metabolites or derivatives which bind to .beta.-lactoglobin and employs as assay material:
- (a) lysine buffer;
- (b) isotope solution;
- (c) .beta.-lactoglobin;
- (d) dextran; and
- (e) charcoal powder;
- the improvement which comprises adding to said assay material a chemical compound of the formula: ##STR5## wherein n is a positive integer of from 1 to 3 and R is radioactive iodine: I.sup.125 or I.sup.131 ; counting for radioactivity and comparing the count with that obtained from a standard curve.
- 4. The method of claim 3 in which the radioactivity is determined by using a gamma scintillation spectrometer.
- 5. The method of claim 3 in which n is 1 and R is I.sup.125.
Parent Case Info
This is a Division of application Ser. No. 495,982 filed Aug. 9, 1974 which has issued into U.S. Pat. No. 3,989,312.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3988431 |
Givas |
Oct 1976 |
|
Non-Patent Literature Citations (1)
Entry |
Grossowicz et al., Blood, The Journal of Hematology, vol. 20, No. 5, Nov. 1962, pp. 609-616. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
495982 |
Aug 1974 |
|