Patent Application
20140123736
The present invention relates to a FP preparing method, a computer-readable storage medium storing an FP preparing program, an FP preparing device, and an FP used for evaluating the quality of an evaluation target, for example, a kampo medicine that is a multicomponent drug.
As evaluation targets, for example, there are natural-product-originated drugs such as kampo medicines that are drugs (hereinafter, referred to as multicomponent drugs) composed of multiple components. Quantitative and qualitative profiles in such drugs change due to a geological factor, an ecological factor, a collecting season, a collecting area, a collecting aetas, weather during the growing period and the like of raw material crude drugs.
Thus, for such multicomponent drugs and the like, predetermined criteria are regulated as qualities for securing the safety and the effectiveness thereof, and national supervising agencies, chemical organizations, manufacturers, and the like perform quality evaluations based on the criteria.
In general, however, the determination criteria on the quality and the like for a multicomponent drug are set based on the content and the like of one or several distinctive components selected from components in the multicomponent drug.
For example, in Non-Patent Literature 1, in a case that effective components of a multicomponent drug are not identified, it selects a plurality of components that have physical properties such as a quantitative analyzability, high water-solubility, undegradability in hot water, and non-chemical reactivity with other components and uses the contents of these components acquired through chemical analysis as evaluation criteria.
In addition, it is known to apply chromatography to a multicomponent drug, obtain an ultraviolet-visible absorption spectrum for each retention time, and set evaluation criteria based on some pieces of component information included therein.
For example, according to Patent Literature 1, some peaks included in HPLC chromatogram data (hereinafter, referred to as a chromatogram) are selected and encoded as barcodes, thereby evaluating a multicomponent drug.
In such methods, however, evaluation targets are limited to “contents of specific components” or “peaks of specific components in chromatogram,” and thus only some components contained in a multicomponent drug are set as the evaluation targets. Accordingly, since a multicomponent drug includes many components other than the components that are the evaluation targets, such methods are insufficient as a method of evaluating the multicomponent drug in terms of accuracy.
In order to accurately evaluate the quality of a multicomponent drug, it is necessary to evaluate a pattern that covers information of all peaks or almost all peaks without small peaks corresponding to several %. Accordingly, it is necessary to associate all the peaks or almost all peaks with each other between multicomponent drugs.
However, it is difficult to efficiently associate a plurality of peaks with high accuracy. This interferes with an efficient evaluation of multicomponent drugs with high accuracy.
Described more, crude drugs being raw materials are natural products, and therefore, multicomponent drugs even which have the same product name may have slightly different components. Hence, even if drugs have the same quality, content ratios of components thereof may be different from each other or a component present in one drug may not be present in the other drug (hereinafter, referred to as an inter-drug error). In addition, there is also a factor that peak intensity or peak elution time in a chromatogram has no precise repeatability (hereinafter, referred to as an analysis error). Accordingly, all peaks or almost all peaks may not be associated with peaks that are originated from the same components between the multicomponent drugs (hereinafter, referred to as peak assignment), thereby interfering with an efficient evaluation with high accuracy.
A problem to be solved is in that there is a limit on an efficient evaluation of the quality of an evaluation target with high accuracy with use of an existing evaluation method.
In order to contribute improvement of the accuracy and the efficiency of an evaluation, the present invention provides a FP preparing method comprising a FP preparing step preparing a FP configured by peaks and retention time points of the peaks detected from a chromatogram of an evaluation target, wherein the chromatogram is a 3D chromatogram including retention time points, detection wavelengths, and peaks as data, and the FP preparing step prepares the FP by the peaks, the retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.
The present invention provides a computer-readable storage medium storing a FP preparing program for an evaluation target that realizes a FP preparing function in a computer preparing a FP configured by peaks and retention time points of the peaks detected from a chromatogram of an evaluation target, wherein the chromatogram is a 3D chromatogram including retention time points, detection wavelengths, and peaks as data, and the FP preparing function prepares the FP by the peaks, the retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.
The present invention provides a FP preparing device for an evaluation target comprising a FP preparing part preparing a FP configured by peaks and retention time points of the peaks detected from a chromatogram of an evaluation target, wherein the chromatogram is a 3D chromatogram including retention time points, detection wavelengths, and peaks as data, and the FP preparing part prepares the FP by the peaks, the retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.
The present invention provides a FP that is prepared using the FP preparing method, wherein the FP is prepared by peaks, retention time points and UV spectra thereof detected from the 3D chromatogram at a specific wavelength.
The FP preparing method for the evaluation target according the present invention has the above-identified configuration, so that it can prepare data (hereinafter, referred to as finger print data: FP) configured by maximum values or area values (hereinafter, peaks) in signal strength (height) of peaks detected from a three dimensional chromatogram data (hereinafter, referred to as a 3D chromatogram) of the evaluation target at a specific wavelength, appearance time points (hereinafter, referred to as retention time points) of the peaks and ultraviolet-visible absorbance spectra (hereinafter, referred to as UV spectra) of the peaks.
This FP is configured by three-dimensional information (peaks, retention time points, and UV spectra) similar to the 3D chromatogram.
Accordingly, the FP is data that directly succeeds to the information unique to the drug. In spite of that, the data volume is highly compressed, so that the amount of information to be processed is much smaller than that of the 3D chromatogram, thereby increasing the processing speed.
As a result, each peak of the target FP can be efficiently assigned to each peak of the reference FP with high accuracy, and accordingly, the accuracy and the efficiency of the evaluation can be improved.
The storage medium storing the FP preparing program according to the present invention has the above-identified configuration, so that it realizes each function in a computer to prepare the FP having three-dimensional information, thereby improving the accuracy and the efficiency of the evaluation.
The FP preparing device according to the present invention has the above-identified configuration, so that it operates each part to prepare the FP having three-dimensional information, thereby improving the accuracy and the efficiency of the evaluation.
The FP according to the present invention has the above-identified configuration, so that the accuracy and the efficiency of the evaluation can be improved.
The object of improving the accuracy and the efficiency of an evaluation is realized by a FP that is prepared as three-dimensional information (peaks, retention time points, and UV spectra).
According to Embodiment 1 of the present invention, there are provided a FP preparing method, a FP preparing program, a FP preparing device, and a FP for an evaluation target such as a multicomponent material, for example, a multicomponent drug.
A multicomponent drug is defined as a drug that contains a plurality of effective chemical components. Examples of the multicomponent drug include a crude drug, a combination of crude drugs, an extract thereof, and a kampo medicine, but are not limited thereto. In addition, the dosage form is not particularly limited, and, examples include a liquid medicine, an extract, a capsule, a granule, a pill, suspension-emulsion, a powder, a spiritus, a tablet, an infusion-decoction, a tincture, a troche, aromatic water, a fluid extract, which are specified in “general rule for preparations” of “The Japanese Pharmacopoeia”, Fifteenth Edition. As the multicomponent material, materials other than a drug are also included.
Specific examples of the kampo medicine are written in Industry Standard and Voluntarily Revision of “Precautions” in 148 Prescriptions for Medical Kampo Drug Formulation and in Guide to General Kampo Prescription (1978).
In an evaluation of a multicomponent drug, it evaluates whether or not an evaluation target drug is equivalent to a plurality of drugs that are defined as normal products. For this, first, a target FP is prepared by extracting information unique to the drug from a three-dimensional chromatogram data (hereinafter, referred to as a 3D chromatogram) of the evaluation target drug.
Next, each peak of the target FP is assigned to peak correspondence data (hereinafter, referred to as a reference group FP) of all reference FPs, which is prepared by performing a peak assigning process to all the reference FPs, whereby a peak feature value is acquired.
Next, equivalency between peaks of the reference group FP and the assigned peaks of the target FP (hereinafter, referred to as target FP assignment peaks) is evaluated by MT method. Finally, it is determined whether or not the evaluation target drug is equivalent to a normal product by comparing an acquired evaluation value (hereinafter, referred to as a MD value) with a preset determination value (an upper limit value of the MD value).
The 3D chromatogram is a HPLC chromatogram data (hereinafter, referred to as chromatogram) of a multicomponent drug that is a multicomponent material as an evaluation target and includes UV spectra.
The FP is fingerprint data that is configured by maximum values or area values (hereinafter, referred to as peaks) in signal strength (height) of peaks detected at a specific wavelength and by appearance time points (hereinafter, referred to as retention time points) of the peaks.
The target FP is acquired by extracting a plurality of peaks, retention time points and UV spectra thereof at a specific detection wavelength from a 3D chromatogram that is three-dimensional chromatogram data of a kampo medicine being an evaluation target.
The reference FP corresponds to the target FP and is a FP of a kampo medicine as a multicomponent drug that is a multicomponent material determined as a normal product.
As illustrated in
In this embodiment, the FP preparing part 3, the reference FP selecting part 5, the peak pattern preparing part 7, the peak assigning part 9, and the evaluating part 11 are configured by a single computer. Alternatively, the FP preparing part 3, the reference FP selecting part 5, the peak pattern preparing part 7, the peak assigning part 9, and the evaluating part 11 may be configured by respective discrete computers, or the FP preparing part 3 and the reference FP selecting part 5, the peak pattern preparing part 7 and the peak assigning part 9, and the evaluating part 11 may be configured by discrete computers.
The FP preparing part 3 is included in a FP preparing device that is configured as a part of the evaluating apparatus 1 for a multicomponent drug and is a functional part that prepares a target FP 17 (hereinafter, simply also referred to as an “FP 17”) according to a FP preparing program that is installed to a computer. The target FP 17 is acquired by extracting a plurality of peaks at a specific detection wavelength, the retention time points, and UV spectra from a 3D chromatogram 15 that is three-dimensional chromatogram data as illustrated in
This FP 17 is configured as three-dimensional information (peaks, retention time points, and UV spectra) similar to the 3D chromatogram 15.
The FP 17, therefore, is data that directly succeed to the information unique to the drug. In spite of that, the data volume of the FP 17 is compressed at the ratio of about 1/70, and therefore, information amount to be processed is much smaller than that of the 3D chromatogram 15, thereby increasing processing speed.
The 3D chromatogram 15 is a result of applying high performance liquid chromatography (HPLC) to a kampo medicine 13 (
Although the detector is not particularly limited, an absorbance detector employing an optical characteristic is used as the detector. A peak is three-dimensionally acquired as a signal strength according to a detection wavelength of ultraviolet (UV). As a detector employing an optical characteristic, a transmittance detector may be used.
The detection wavelengths are not particularly limited, and are a plurality of wavelengths selected preferably from a range of 150 nm to 900 nm, selected more preferably from a range of 200 nm to 400 nm corresponding to a UV-visible absorption range, and selected further more preferably from a range of 200 nm to 300 nm.
The 3D chromatogram 15 at least includes a number (lot number), retention time points, detection wavelengths, and peaks of a kampo medicine as data.
In addition, the 3D chromatogram 15 can be also acquired by using commercially-available devices. As such a commercially-available device, there is “Agilent 1100 system” or the like. Furthermore, the chromatograph is not limited to the HPLC, and any other type of chromatography may be employed.
In the 3D chromatogram 15, as illustrated in
The FP 17 at least includes a number (lot number), retention time points, peaks at a specific wavelength, and UV spectra of a kampo medicine as data.
The FP 17 is two-dimensionally represented with the x-axis representing the retention time points and the y-axis representing the peaks for the specific detection wavelength as illustrated in
Meanwhile, there are cases where all the peaks are not included at a single wavelength. In such a case, a plurality of detection wavelengths are set to prepare a FP that includes all the peaks by combining the plurality of wavelengths as described later.
In Embodiment 1, although the peak is set as the maximum value of the signal strength (peak height), the area value may be used as the peak. In addition, a FP may not include UV spectra, so that the FP is set as two-dimensional display information in which the x-axis represents the retention time points, and the y-axis represents the peaks for a specific wavelength. In such a case, the FP can be prepared from a 2D chromatogram as a chromatogram that includes a number (lot number) and retention time points of a kampo medicine as data.
The reference FP selecting part 5 is a functional part that selects a reference FP that is used by the peak pattern preparing part 7 from among a plurality of reference FPs. The reference FP selecting part 5 selects a FP of a multicomponent material that is appropriate to the assignment of the peaks to the target FP from among the plurality of reference FPs. In other words, in order to perform peak assignment of each peak of the target FP with high accuracy, as illustrated in
In addition, the peak pattern preparing part 7 is a functional part that, as illustrated in
The allowable range is not particularly limited, but is preferably in the range of 0.5 to 2 minutes with the object of the accuracy and efficiency. In Embodiment 1, the allowable range is set to one minute.
In addition, the peak pattern preparing part 7 is configured to be able to flexibly respond to even a case where there is a difference between the number of the peaks of the target FP 33 and that of the reference FP 55 (in other words, there are one or more peaks that are not present on one side). For this, as illustrated in
The peak assigning part 9 is a functional part that compares the individual peak patterns of the target FP and the reference FP to specify corresponding peaks. In the embodiment, the corresponding peaks are specified by calculating the degree of matching between peak patterns for assignment target peaks and assignment candidate peaks and the degree of matching between the UV spectra. It will be described specifically later.
In addition, the peak assigning part 9 is a functional part that calculates the degrees of matching for the assignment candidate peaks by integrating aforementioned two kinds of the degrees of matching to assign each peak of the target FP 33 to each peak of the reference FP 55 based on the calculated degrees of matching.
Furthermore, the peak assigning part 9 is a functional part that finally assigns the peaks of the target FP to respective peaks of the reference group FP as illustrated in
The peak assigning part 9 calculates the degree of matching between peak patterns based on differences between corresponding peaks and retention time points of the peak patterns of the assignment target peak and the assignment candidate peak as illustrated in
The evaluating part 1 is a functional part that evaluates the peaks that are specified and assigned by the peak assigning part 9 by comparison with the peaks of the plurality of reference FPs. In the embodiment, the evaluating part 11 is a functional part that evaluates the equivalency between the target FP assignment peaks 21 and the reference group FP 19 with MT method.
MT method represents a calculation technique that is generally known in quality engineering. For example, MT method is described in pp 136 to 138, “Mathematics for Quality Engineering” published by Japanese Standards Association (2000); in pp 454 to 456 of Quality Engineering of Application Course “Technical Developments in Chemistry, Pharmacy and Biology” published by Japanese Standards Association (1999); in pp 78 to 84 of Quality Engineering 11(5) (2003); and in “Introduction to MT System” (2008).
In addition, MT method program software that is commercially available in the market can be used. As such commercially-available MT method program software, there are “ATMTS” provided by Angle Try Associates, “TM-ANOVA” provided by Japanese Standards Association, an “MT method for Windows” provided by OHKEN Co., Ltd, and the like.
The evaluating part 11 assigns a variable axis according to MT method to one of the lot number and the retention time point of a kampo medicine or the UV detection wavelength of the target FP 17 and sets the peaks as feature values according to MT method.
Although the assignment of the variable axis is not particularly limited, it is preferable that the retention time point is assigned to a so-called category axis according to MT method, the number of a multicomponent-based drug is assigned to a so-called number row axis, and the peak is assigned to a so-called feature value according to MT method.
Here, the category axis and the number row axis are defined as below. According to MT method, an average value mj and a standard deviation σj are acquired for a data set Xij, a correlation coefficient “r” between “i” and “j” is acquired from a value xij=(Xij−mj)/σj that is the standardized Xij, and accordingly, a unit space or a Mahalanobis distance is acquired. At this time, the category axis and the number row axis are defined such that “the average value mj and the standard deviation σj are acquired for each value of the category axis by changing the value of the number row axis.”
Based on the data and the feature values to which the axes are assigned, a reference point and an unit quantity (it may be abbreviated as a “unit space”) are acquired using MT method. Here, the reference point, the unit quantity, and the unit space are defined in accordance with the description of MT method presented in the above-described literatures.
According to MT method, an MD value is acquired as a value that represents the degree of a difference between a drug to be evaluated and the unit space. Here, the MD value is defined in the same way as the description of MT method presented in the literatures, and the MD value is acquired with the method described in the literatures.
By using the MD value acquired in this manner, the drug to be evaluated can be evaluated by determining the degree of a difference from a plurality of drugs defined as normal products.
For example, by performing the assignment process for each target FP illustrated in
When this MD value is evaluated with respect to an MD value of a normal product, MD values are similarly acquired for a plurality of drugs defined as normal products. A threshold value is set from the MD values of these normal products, the MD value of the evaluation target drug is plotted as an evaluation result 23 of the evaluating part 11 illustrated in
In addition, it is sufficient for the evaluating part 11 to be able to compare and evaluate the equivalency between the target FP assignment peaks 21 and the reference group FP 19, and therefore, a pattern recognition technique other than MT method or the like can be used.
The function of the above-described reference FP selecting part 5 will be further described with reference to
In the peak assigning process for the target FP 33, the peaks of the target FP 33 are assigned to a reference FP whose FP pattern is closest to the target FP 33 as much as possible. Selecting this reference FP that is closest to the target FP 33 from among a plurality of reference FPs is an important point for performing assignment with high accuracy.
Thus, as a method of evaluating similarity to a FP pattern of the target FP 33 in an objective and simplified manner, the similarity of the FP pattern is evaluated based on the degree of matching between the retention time appearance patterns.
For example, in a case where the retention time points of the target FP 33 and the reference FP 55 are as illustrated in
In
Accordingly, an inter-retention time point distance between the peaks 35 and 37 is (10.5)−(10.2)=(0.3). Similarly, a distance between the peaks 35 and 39 is (0.6), a distance between the peaks 37 and 39 is (0.3), etc. The followings are similarly acquired and a target FP appearance pattern 79 is formed into a table on the lower side of
In
Accordingly, in the same way, inter-retention time point distances form a reference FP appearance pattern 81 into a table on the lower side of
The individual peaks patterned as illustrated in
Namely, all the inter-retention time point distances of the retention time appearance patterns of the target FP 33 and the reference FP 55 are sequentially compared with each other in units of rows in a round-robin system, thereby calculating the number of the distances that match within a set range.
For example, comparing the first rows of the target and reference FP retention time appearance patterns 79 and 81 in
The results are represented in
When the degree of matching between retention time appearance patterns is RP, a degree (RPfg) of matching between a retention time appearance pattern of the f-th row of the target FP 33 and a retention time appearance pattern of the g-th row of the reference FP 55 is calculated using Tanimoto coefficient as:
RP
fg={1−(m/(a+b−m))}×(a−m+1).
In the equation, “a” is the number of peaks of the target FP 33 (the number of target FP peaks), “b” is the number of peaks of the reference FP 55 (the number of reference FP peaks), and “m” is the number of matches in the appearance distance (see
A minimum value (RP_min) of these RPs is set as the degree of matching between the retention time appearance patterns of the target FP 33 and the reference FP 55. In the case of
The degrees of matching are calculated for all the reference FPs, and a reference FP having the smallest degree of matching is selected, and the peaks of the target FP are assigned to the reference FP.
The reference FP selecting part 5 may pattern the target FP 33 and the reference FP 55 at peak heights ratios.
The peaks patterned with use of the peak height ratios are compared in a round-robin system, to calculate the number of matches in the height ratio within a set range. By performing the calculation, similarly to the case of
In addition, in the case where the peaks are patterned at the peak height ratios, there is a case where a plurality of similar values are present in one row, and thus these values are required not to be counted a plurality of times.
The degree of matching can be acquired by setting the Tanimoto coefficient as “the number of matches in height ratio/(the number of target FP peaks+the number of reference FP peaks−the number of matches in the height ratio)” and approaching (1−Tanimoto coefficient) to zero.
In addition, (1−Tanimoto coefficient) is weighted by (the number of target FP peaks−the number of matches in height ratios+1) to be “(1−Tanimoto coefficient)×(the number of target FP peaks−the number of matches in the appearance distance or the height ratio+1”, whereby a reference FP that matches more peaks (35, 37, . . . ) of the target FP 33 in accordance with the weighting can be selected.
Feature Value Prepared in Accordance with Peak Pattern
The functions of the peak pattern preparing part 7 and the peak assigning part 9 will be described further with reference to
When the assignment target peak 45 is assigned to one of peaks of the reference FP 55, it works out to that the peak should be assigned to which one of the peaks as illustrated in
In addition, as illustrated in
However, even in a case where the peak assignment is performed based on the three kinds of information, UV spectra with similar components are the almost same as the characteristics. Accordingly, if a plurality similar components are included in the assignment candidate peaks, the assignment is consequently performed based on only peak information, whereby sufficient accuracy cannot be acquired. Hence, in order to perform peak assignment with high accuracy, more information is necessary to be added to the three kinds of information.
Then, peak patterns including information of peripheral peaks as illustrated in
If the peak pattern includes the peripheral peaks, the peripheral information is added to the prior three kinds of information. Accordingly, the peak assignment can be performed based on four kinds of information, whereby higher assignment accuracy can be acquired.
As a result, massive peaks can be efficiently assigned all together through one assignment process with high accuracy.
In addition, by configuring data used for the peak assignment as the four kinds of information including the peripheral information, there is no need of restriction conditions (definition of peaks and the like) to be set in a conventional peak assignment process.
In the case illustrated in
In the case illustrated in
In the cases of
In the case of
In the cases of
In order to compare peak patterns with higher accuracy, it is important to prepare a peak pattern in which the numbers of peripheral peaks are increased for both the target FP and the reference FP as illustrated in
For example, by comparing peak patterns having a total of five peaks that includes four peripheral peaks, higher assignment accuracy is acquired.
In the case of
In the cases of
In addition, in order to perform the assignment according to the peak patterns with higher accuracy, it is necessary to respond to a case in which there is a difference between the number of peaks of the target FP and the number of peaks of the reference FP (in other words, there is a peak that is not present on one side). For this, it is important to prepare peak patterns in which peak pattern configuring peaks are comprehensively changed for both the assignment target peak and the assignment candidate peak, as illustrated in
More specifically, peaks being candidates for the peak pattern configuring peak (hereinafter, peak pattern configuring candidate peaks) are set from among peripheral peaks of the assignment target peak of the target FP in advance. Peak patterns are prepared by setting the peak pattern configuring candidate peaks as the peak pattern configuring peak in turns. Also for the assignment candidate peaks of the reference FP, similarly, peak pattern configuring candidate peaks are set to prepare peak patterns are by setting the peak pattern configuring candidate peaks as the peak pattern configuring peak in turn.
For example, as illustrated in
In addition, in a case where ten peak pattern configuring candidate peaks are set, arbitrary two peak pattern configuring peaks are set, and peak patterns of 10C2 (=45) patterns are prepared for each one of the assignment target peak and the assignment candidate peak. In a case where arbitrary four peaks are set as the peak pattern configuring peaks, peak patterns of 10C4 (=210) patterns are prepared for each one of the assignment target peak and the assignment candidate peak.
The function of the peak assigning part 9 will be described further with reference to
The peak assigning part 9 calculates the degree of matching between peak patterns (hereinafter, referred to as P_Sim) based on differences in corresponding peaks and retention time points over all the peak patterns for the assignment target peak and the assignment candidate peaks prepared by the peak pattern preparing part 7. The peak assigning part 9 sets the minimum value of the P_Sim (hereinafter, referred to as P_Sim_min) as the degree of matching between peak patterns for the assignment target peak and the assignment candidate peak.
For example, as illustrated in
Incidentally, in a case where ten peak pattern configuring candidate peaks located in front and in the rear in the time axis direction are set and the peak pattern configuring peaks are set as two arbitrary peaks for each one of the assignment target peak 45 and the assignment candidate peak 65, peak patterns of 4C2(=45) patterns are prepared for each one of the assignment target peak and the assignment candidate peak. Accordingly, the P_Sims of the assignment target peak 45 and the assignment candidate peak 65 are calculated as 45 patterns×45 patterns (=2025), and the P_Sim_min that is the minimum value of the P_Sims is set as the degree of matching between the assignment target peak 45 and the assignment candidate peak 65. In addition, in a case where the peak pattern configuring peaks are set as four arbitrary peaks, peak patterns of 10C4 (=210) patterns are prepared for each one of the assignment target peak and the assignment candidate peak. Accordingly, the P_Sims of the assignment target peak 45 and the assignment candidate peak 65 are calculated as 210 patterns×210 patterns (=44100), and the P_Sim_min that is the minimum value of the P_Sims is set as the degree of matching between the assignment target peak 45 and the assignment candidate peak 65.
The P_Sim is similarly calculated for all the assignment candidate peaks for the assignment target peak 45.
A calculating method of the degree of matching between peak patterns for comparing peak patterns each configured by three peaks will be described with reference to
In the peak pattern 87 of the assignment target peak 45, peak data and a retention time point of the assignment target peak 45 are assumed to be p1 and r1, peak data and a retention time point of a peak pattern configuring peak 43 are assumed to be dn1 and cn1, and peak data and a retention time point of a peak pattern configuring peak 47 are assumed to be dn2 and cn2.
In the peak pattern 91 of the assignment candidate peak 67, peak data and a retention time point of the assignment candidate peak 67 are assumed to be p2 and r2, peak data and a retention time point of a peak pattern configuring peak 65 are assumed to be fn1 and en1, and peak data and a retention time point of a peak pattern configuring peak 69 are assumed to be fn2 and en2.
When the degree of matching between peak patterns is P_Sim, the degree of matching between peak patterns (P_Sim(45-67)), each configured by three peaks, of the assignment target peak 45 and the assignment candidate peak 67 is calculated as:
Here, d represented in the equation is a value used for correcting the deviation of the retention time point.
The calculating method of the degree of matching between peak patterns used for comparing the peak patterns each configured by five peaks will be described with reference to
In the peak pattern 97 of the assignment target peak 45, peak data and a retention time point of the assignment target peak 45 are assumed to be p1 and r1, and peak data and retention time points of peak pattern configuring peaks 41, 43, 47, and 49 are assumed to be dn1 and cn1, dn2 and cn2, dn3 and cn3, and dn4 and cn4.
In the peak pattern 101 of the assignment candidate peak 67, peak data and a retention time point of the assignment candidate peak 67 are assumed to be p2 and r2, and peak data and retention time points of peak pattern configuring peaks 63, 65, 69, and 71 are assumed to be fn1 and en1, fn2 and en2, fn3 and en3, and fn4 and en4.
The degree of matching between peak patterns (P_Sim(45-67)) each configured by five peaks, of the assignment target peak 45 and the assignment candidate peak 67 is calculated as:
Here, d represented in the equation is a value used for correcting the deviation of the retention time point.
The peak assigning part 9 calculates the degree of matching between the UV spectra of the assignment target peak and the assignment candidate peak as illustrated in
UV
—
Sim(45-67)=RMSD(107 vs 111).
The RMSD is defined as a mean square deviation and is defined as the square root of arithmetic average of a value that is a square of a distance between two corresponding points (dis). In other words, RMSD is calculated as √{Σdis2/n}.
“n” is the number of “dis.”
Here, the waveform of the UV spectrum has a maximum wavelength and a minimum wavelength, and the degree of matching also can be calculated by comparing either the maximum wavelengths or the minimum wavelengths. However, compounds having no absorbance property or compounds having similar absorbance properties, they may quite differs from each other in the waveforms as a whole while having the same maximum and minimum wavelengths. Accordingly, there is a risk that the degree of matching between the waveforms may not be calculated by comparing either the maximum wavelengths or the minimum wavelengths.
In contrast to this, in a case where the RMSD is used in accordance with the waveforms of the UV spectra, the whole waveforms are compared with each other.
Therefore, the degree of matching between the waveforms of the UV spectra can be calculated with accuracy, whereby even compounds having no absorbance property or compounds having similar absorbance properties can be identified with accuracy.
The degree of matching between the UV spectra is calculated similarly for all the assignment candidate peaks of the assignment target peak 45.
In addition, the peak assigning part 9 calculates the degree of matching of the assignment candidate peaks that is acquired by integrating the above-described two degrees of matching as illustrated in
As illustrated in
SCORE(45-67)=P—Sim_min(45-67)×UV—Sim(45-67).
The degree of matching of assignment candidate peaks is similarly calculated for all the assignment candidate peaks for the assignment target peak 45.
Then, the SCOREs of all the assignment candidate peaks are compared to determine an assignment candidate peak having a lowest SCORE as an assignment peak of the assignment target peak 45.
Since the peak assigning part 9 determines the peaks to which the assignment target peaks should be assigned by integrating two viewpoints, it can realize peak assignment with accuracy.
In addition, the peak assigning part 9 assigns each peak of the target FP 17 to the reference group FP 19 based on the result of the assignment of the target FP to the reference FP as illustrated in
Each peak of the target FP 17 is assigned to the reference F′ configuring the reference group FP through the above-described assignment process. Base on the result of the assignment, finally, the peaks are assigned to the reference group FP 19.
In addition, the reference group FP 19 is prepared by performing an assignment process like the above for the plurality of reference FPs determined as normal products, and each peak is represented by an average value (black point) of assigned peaks±standard deviation (vertical line).
From this result, the MD value (MD values: 0.25, 2.99, and the like) can be acquired by MT method (see
As illustrated in
The above-described FP preparing step 113 is provided as the FP preparing method, sets the 3D chromatogram that has data of retention time points, detection wavelengths, and peaks as the chromatogram, and prepares the FP 17 by the peaks, the retention time points, and the UV spectra of the peaks detected from this 3D chromatogram 15 at a specific wavelength.
The FP preparing function is realized in Step S1, the reference FP selecting function is realized in Step S2, the peak pattern preparing function is realized in Step S3, the peak assigning function is realized in Steps S3 to S5, and the evaluating function is realized in Steps S6 and S7.
In Step S1, the “FP preparing process” is performed with a 3D chromatogram and peak information at a specific detection wavelength as input data.
The 3D chromatogram is data that is acquired by analyzing an evaluation target drug through HPLC and it is configured as three-dimensional information including a retention time points, detection wavelengths, and peaks (signal strength) as represented as a data example 123 of the 3D chromatogram in
In Step S1, the FP preparing part 3 (
In Step S2, the “target FP assigning process 1” is performed with input of the target FP and all the reference FPs output in Step S1.
In Step S2, the reference FP selecting part 5 of the computer functions to calculate the degree of matching between retention time appearance patterns of all the reference FPs with respect to the target FP 17, to select a reference FP that is appropriate to the assignment of the target FP 17.
The reference FPs are FPs prepared by the same process as that of Step S1 based on the 3D chromatogram and peak information of drugs determined as normal products. In addition, the normal product is defined as a drug of which the safety and the effectiveness are checked and a plurality of drugs with different product lots correspond thereto. The reference FP is data configured similarly to the FP data example 127 in
In Step S3, the “target FP assigning process 2” is performed according to the target FP 17 and the reference FP selected in Step S2 as input.
In Step S3, the peak pattern preparing part 7 (
In Step S4, the “target FP assigning process 3” is performed according to the determination result file output in Step S3 as an input.
In Step S4, the peak assigning part 7 of the computer functions to, between the target FP 17 and the reference FP, specify peaks of the reference FP that correspond to the respective peaks of the target FP based on the degree (SCORE) of matching of the assignment candidate peaks and outputs the result in the form of a file (collation result file).
In Step S5, the “target FP assigning process 4” is performed according to the collation result file output in Step S4 and the reference group FP as inputs.
The reference group FP is peak correspondence data over all the reference FPs prepared from the all reference FPs in the same process as that of Steps S2 to S4.
In Step S5, the peak assigning part 7 of the computer functions to assign the peaks of the target FP 17 to the respective peaks of the reference group FP based on the collation result file of the target FP 17 as illustrated in
In Step S6, the “FP evaluating process” is performed according to the peak data feature value file output in Step S5 and the reference group FP as inputs.
In Step S6, the evaluating part 11 of the computer functions to evaluate the equivalency between the peak data feature value data output in Step S5 and the reference group FP by MT method, and outputs the evaluation result as an MD value (
In Step S7, the “determination of a success or not” is performed according to the MD value output in Step S6 as input.
In Step S7, the evaluating part 11 of the computer functions to compare the MD value output in Step S6 with a threshold value (the upper limit of the MD value) set in advance so as to make a decision to pass or fail (Graph 23 illustrated in
In Step S101, a process of “reading peak information” is performed. In this process, peak information is read out as the first one of two kinds of data that are necessary for preparing a FP, and it proceeds to Step S102.
In Step S102, a process of “sequentially acquiring a retention time point (R1) of a peak and peak data (P1) corresponding thereto” is performed. In this process, retention time points (R1) and peak data (P1) of the peaks are sequentially acquired from the peak information one by one, and it proceeds to Step S103.
In Step S103, a process of “reading a 3D chromatogram” is performed. In this process, a 3D chromatogram is read as the second one of the two kinds of data necessary for preparing the FP, and it proceeds to Step S104.
In Step S104, a process of “sequentially acquiring a retention time point (R2) of a peak and a UV spectrum (U1) corresponding thereto” is performed. In this process, retention time points (R2) and UV spectra (U1) are acquired from the 3D chromatogram at each period that is a half of a sampling rate at the time of analyzing the HPLC, and it proceeds to Step S105.
In Step S105, a determining process “|R1−R2|≦Threshold Value?” is performed. In this process, it is determined whether or not the retention time points R1 and R2 read in Steps S102 and S104 correspond to each other within a threshold value range. If corresponding (YES), it is determined that two retention time points are the same and the UV spectrum of the peak at the retention time point R1 is U1. Then, it proceeds to Step S106. If not corresponding (NO), it is determined that the two retention time points are not the same and the UV spectrum of the peak at the retention time point of R1 is not the UV spectrum U1. Then, it proceeds to Step S104 so as to perform comparison with the next data of the 3D chromatogram. The threshold value used in this determination process is the “sampling rate” of the 3D chromatogram.
In Step S106, a process of “normalizing the UV spectrum U1 with the maximum value of “1”” is performed. In this process, the UV spectrum U1 determined as the UV spectrum of the retention time point R1 in Step S105 is normalized with the maximum value of “1”, and it proceeds to Step S107.
In Step S107, a process of “outputting R1 and P1 as well as the normalized U1 (target FP)” is performed. In this process, the R1 and P1 acquired from the peak information and the U1 normalized in S106 are output to the target FP, and it proceeds to Step S108.
In Step S108, a determining process “Has the process for all the peaks been completed?” is performed. In this process, it is determined whether or not all the peaks included in the peak information have been processed. If the process has not been completed for all the peaks (NO), it proceeds to Step S102 in order to process one or more peaks that have not been processed. The process of Steps S102 to S108 is repeated until the process of all the peaks is completed. If the process of all the peaks has been completed (YES), the FP preparing process is finished.
This FP preparing process is for preparing a FP that covers all the peaks of the 3D chromatogram with use of peak information of a plurality of wavelengths in a case where all the peaks detected in the 3D chromatogram cannot be covered at the single wavelength as illustrated in
In addition,
In Step S110, a process of “preparing a FP for each wavelength” is performed. In this process, the above-described FP preparing process using only the single wavelength is performed for each wavelength so as to prepare n FPs, and it proceeds to Step S111.
In Step S111, a process of “listing the FPs according to the number of peaks (descending order)” is performed. In this process, the n FPs are listed in the descending order of the number of peaks, and it proceeds to Step S112.
In Step S112, as initialization of a counter for sequentially processing n FPs, one is substituted into n (n←1), and it proceeds to Step S113.
In Step S113, a process of “reading the n-th FP in the list” is performed. In this process, the n-th FP in the list is read, and it proceeds to Step S114.
In Step S114, a process of “acquiring all the retention time points (X)” is performed. In this process, all the retention time point information of the FPs read in S113 is acquired, and it proceeds to Step S115.
In Step S115, a process of “updating n (n←n+1)” is performed. In this process, “n+1” is substituted into “n” as the update of “n” in order to transfer the process to the next FP, and it proceeds to Step S116.
In Step S116, a process of “reading the n-th FP in the list” is performed. In this process, the n-th FP in the list is read, and it proceeds to Step S117.
In Step S117, a process of “acquiring all the retention time points (Y)” is performed. In this process, the retention time point information of all the FPs read in S116 is acquired, and it proceeds to Step S118.
In Step S118, a process of “integrating X and Y without duplication (Z)” is performed. In this process, the retention time point information X acquired in S114 and retention time point information Y acquired in Step S117 are integrated without duplication, thereafter, the integrated information is stored in Z, and it proceeds to Step S119.
In Step S119, a process of “updating X (X←Z)” is performed. In this process, as the update of X, Z stored in Step S118 is substituted for X, and it proceeds to Step S120.
In Step S120, a determining process “Have all the FPs been processed?” is performed. In this process, it is determined whether or not all the n FPs prepared in Step S110 have been processed. If processed (YES), it proceeds to Step S121. If there are one or more FPs that have not been processed (NO), it proceeds to Step S115 in order to perform the process of Steps S115 to S120 for the FPs that have not been processed. Until the process of all the FPs are completed, the process of Steps S115 to S120 is repeated.
In Step S121, as the initialization of the counter for sequentially processing n FPs, “1” is substituted into “n” (n←1), and it proceeds to Step S122.
In Step S122, a process of “reading the n-th FP in the list” is performed. In this process, the n-th FP in the list is read, and it proceeds to Step S123.
In Step S123, a process of “sequentially acquiring a retention time point (R1), peak data (P1), and a UV spectrum (U1) of each peak” is performed. In this process, retention time points (R1), peak data pieces (P1), and UV spectra (U1) of peaks are sequentially acquired from the FP read in Step S122 one by one, and it proceeds to Step S124.
In Step S124, a process of “sequentially acquiring retention time points (R2) from X” is performed. In this process, retention time points (R2) are sequentially acquired from X in which the retention time points of all the FPs are stored without duplication one by one, and it proceeds to Step S125.
In Step S125, a determining process “R1=R2?” is performed. In this process, it is determined whether or not R1 acquired in Step S123 and R2 acquired in Step S124 are the same. If being the same (YES), it proceeds to Step S127. If not being the same (NO), it proceeds to Step S126.
In Step S126, a determining process “Has the comparison of all the retention time points of X been completed?” is performed. In this process, it is determined whether or not the comparison of R1 acquired in S123 with all the retention time points of X has been completed. If completed (YES), it is determined that the peak at the retention time point of R1 has been processed and it proceeds to Step S123 in order to transfer the process to the next peak. If not completed (NO), it proceeds to Step S124 in order to transfer the process to the next retention time point of X.
In Step S127, a process of “adding (n−1)×analysis time (T) to R1 (R1←R1+(n←1)×T)” is performed. In this process, for each retention time point that is present in the first FP, which has the highest number of peaks, in the list, the retention time point is unchanged. For the retention time of a peak that is not present in the 1st FP in the list but is present in the 2nd FP, an analysis time (T) is added to R1. For the retention time of a peak that is not present in the 1st to (n−1)-th FP in the list but is present in the n-th FP, (n−1)×T is added to R1. Then, it proceeds to Step S128.
In Step S128, a process of “outputting R1, P1, and U1 (target FP)” is performed. In this process, R1 processed in Step S127, P1 and U1 acquired in Step S123 are output to the target FP, and it proceeds to Step S129.
In Step S129, a process of “removing R2 from X” is performed. In this process, since the process at the retention time points R1(=R2) have been completed in Steps S127 and S128, the retention time points (R2) that have been processed are removed from X, and it proceeds to S130.
In Step S130, a determining process “Have all peak processes been completed?” is performed. In this process, it is determined whether or not the process has been completed for all the peaks of the n-th FP in the list. If completed (YES), the FP preparing process for the n-th FP in the list is finished to proceed to Step S131. If not completed (NO), it proceeds to Step S123 in order to process any peak that has not been completed. Until the process of all the peaks is finished, the process of Steps S123 to S130 is repeated.
In Step S131, a process of “updating n (n←n+1)” is performed. In this process, in order to transfer the process to the next FP, “n+1” is substituted into “n” as the update of “n” to proceed to Step S132.
In Step S132, a determining process “Have all FP processes been completed?” is performed. In this process, it is determined whether or not all the n FPs prepared in Step S110 have been processed. If processed (YES), the FP preparing process is finished. If there are one or more FPs that have not been processed (NO), it proceeds to Step S122 in order to perform the process of Steps S122 to S132 for the FPs that have not been processed. Until the process of all the FPs is completed, the process of Steps S122 to S132 is repeated.
In Step S201, a process of “reading a target FP” is performed. In this process, the FP that is an assignment target is read, and it proceeds to Step S202.
In Step S202, a process of “acquiring all the retention time points (R1)” is performed. In this process, all the retention time point information of the target FP that is read in S201 is acquired, and it proceeds to Step S203.
In Step S203, a process of “listing file names of all the reference FPs” is performed. In this process, file names of all the reference FPs are listed in advance in order to sequentially process all the reference FPs later, and it proceeds to Step S204.
In Step S204, “1” is substituted into “n” (n←1) as an initial value of the counter used for sequentially processing all the reference FPs, and it proceeds to Step S205.
In Step S205, a process of “reading the n-th reference FP (reference FPn) in the list” is performed. In this process, the n-th FP of the file name list of all the reference FPs listed in Step S203 is read, and it proceeds to Step S206.
In Step S206, a process of “acquiring all the retention time points (R2)” is performed. In this process, all of the retention time point information of the reference FP that are read in S205 are acquired, and it proceeds to Step S207.
In Step S207, a process of “calculating the degree of matching between retention time appearance patterns of R1 and R2 (RPn
In Step S208, a process of “storing RPn
In Step S209, a process of “updating n (n←n+1)” is performed. In this process, in order to transfer the process to the next FP, “n+1” is substituted for n as the update of n, and it proceeds to Step S210.
In Step S210, a determining process “Have all reference FP processes been completed?” is performed. In this process, it is determined whether or not all the reference FPs have been processed. If processed (YES), it proceeds to Step S211. If there are one or more reference FPs that have not been processed (NO), it proceeds to Step S205 in order to perform the process of Steps S205 to S210 for the FPs that have not been processed. Until the process of all the reference FPs are completed, the process of Steps S205 to S210 is repeated.
In Step S211, a process of “selecting a reference FP demonstrating the minimum degree of matching from RPall
In Step S301, a process of “reading a target FP” is performed. In this process, the FP that is an assignment target is read, and it proceeds to Step S302.
In Step S302, a process of “sequentially acquiring a retention time point (R1), peak data (P1), and a UV spectrum (U1) of an assignment target peak” is performed. In this process, the peaks of the target FP read in Step S301 are sequentially set as the assignment target peak to acquire R1, P1, and U1, and it proceeds to Step S303.
In Step S303, a process of “reading the reference FP” is performed. In this process, the reference FP that is selected in the “Target FP Assigning Process 1” in
In Step S304, a process of “sequentially acquiring a retention time point (R2), peak data (P2), and a UV spectrum (U2) of a peak of the reference FP” is performed. In this process, R2, P2, and U2 are acquired from the reference FP read in Step S303 for each peak, and it proceeds to Step S305.
In Step S305, a determining process “|R1−(R2+d)|<Threshold Value?” is performed. In this process, it is determined whether or not R1 and R2 read in Steps S302 and S304 correspond to each other within the threshold value range. If corresponding (YES), it is determined that the peak of which the retention time point is R2 is an assignment candidate peak of the peak of which the retention time point is R1. Then, in order to calculate the degree of matching for the assignment candidate peak (SCORE), it proceeds to Step S306. If not corresponding (NO), since the peak of which the retention time point is R2 and the peak of which the retention time point is R1 have a great difference in the retention time, it is determined that the peak cannot be set as the assignment candidate peak, and it proceeds to Step S309. In addition, “d” used in this determination process is a value for correcting the retention time points of the peaks of the target FP and the reference FP, and the initial value is set to zero. A difference between retention time points of peaks is acquired whenever being assigned during the progress of the process to update “d” with the value. In addition, the threshold value is an allowable range of the retention time points used for determining whether to be set as an assignment candidate peak.
In Step S306, a process of “calculating the degree of matching between UV spectra (UV_Sim)” is performed. In this process. UV_Sim is calculated based on U1 of the assignment target peak acquired in Step S302 and U2 of the assignment candidate peak acquired in S304, and it proceeds to Step S307. In addition, a detailed calculation flow of UV_Sim will be described with reference to “Subroutine 2” in
In Step S307, a process of “calculating the degree of matching between peak patterns (P_Sim_min)” is performed. In this process, from R1 and P1 of the assignment target peak acquired in Step S302 and R2 and P2 of the assignment candidate peak acquired in Step S304, peak patterns are comprehensively prepared for these peaks. In addition, P_Sim_min of these peak patterns is calculated, and it proceeds to Step S308. A detailed calculation flow of P_Sim_min will be described with reference to “Subroutine 3” in
In Step S308, a process of “calculating the degree of matching for the assignment candidate peak (SCORE)” is performed. In this process, from UV_Sim calculated in Step S306 and P_Sim_min calculated in Step S307, SCORE of the assignment target peak and the assignment candidate peak is calculated as:
SCORE=UV—Sim×P—Sim_min.
It proceeds to Step S310.
In Step S309, a process of “substituting “888888” into SCORE (SCORE←888888)” is performed. In this process, SCORE of a peak of an assignment target peak that does not correspond to an assignment candidate peak is set to “888888”, and it proceeds to Step S310.
In Step S310, a process of “storing SCORE (SCORE_all)” is performed. In this process, SCORE acquired in Step S308 or S309 is stored in the SCORE_all, and it proceeds to Step S311.
In Step S311, a determining process “Has the process of all reference peaks been completed?” is performed. In this process, it is determined whether or not all the peaks of the reference FP have been processed. If processed (YES), it proceeds to Step S312. If there are one or more peaks that have not been processed (NO), it proceeds to Step S304 in order to perform the process of S304 to S311 for the unprocessed peaks. Until the process of all the peaks is completed, the process of Steps S304 to S311 is repeated.
In Step S312, a process of “outputting the SCORE_all to a determination result file to initialize (vacate) the SCORE_all” is performed. In this process, the SCORE_all is output to the determination result file, and thereafter, the SCORE_all is initialized (vacated), and it proceeds to Step S313.
In Step S313, a determining process “Has the process of all target peaks been completed?” is performed. In this process, it is determined whether all the peaks of the target FP have been processed. If processed (YES), the target FP assigning process 2 is finished. If there are one or more peaks that have not been processed (NO), it proceeds to Step S302 in order to perform the process of Steps S302 to S313 for the unprocessed peaks. Until the process of all the peaks is completed, the process of S302 to S313 is repeated.
In Step S401, a process of “reading the determination result file” is performed. In this process, the determination result file prepared by the “target FP assigning process 2” in
In Step S402, a process of “preparing an assignment candidate peak score table with data satisfying the condition of “SCORE<Threshold value”” is performed. In this process, an assignment candidate score table 131 is prepared in
In Step S403, a process of “preparing an assignment candidate peak number table” is performed. In this process, an assignment candidate peak number table 133 illustrated in
In Step S404, a process of “acquiring the peak numbers of the target FP to be assigned” is performed. In this process, a peak number of the target FP that is located at the highest position is acquired for each peak of the reference FP from the assignment candidate peak number table prepared in Step S403, and it proceeds to Step S405.
In Step S405, a determining process “Are the acquired peak numbers aligned in a descending order (without duplication)?” is performed. In this process, it is determined whether or not the peak numbers of the target FP acquired in Step S404 are aligned in the descending order without duplication. If aligned (YES), it is determined that the peaks of the target FP corresponding to respective peaks of the reference FP can be settled, and it proceeds to Step S408. If not aligned (NO), in order to reconsider one or more problematic peaks of the target FP to be assigned to peaks of the reference FP, it proceeds to Step S406.
In Step S406, a process of “comparing SCOREs of problematic peaks to update the assignment candidate peak number table” is performed. In this process, SCOREs corresponding to the peak numbers of the target FP that have the problem are compared with use of the assignment candidate score table, and the assignment candidate peak number table is updated in which a peak number having a larger SCORE is substituted into a peak number located in the second, and it proceeds to Step S407.
In Step S407, a process of “updating the assignment candidate peak store table” is performed. In this process, in accordance with the updated content of the assignment candidate peak number table in Step S406, the assignment candidate peak score table is updated, and it proceeds to Step S404. Until there is no problem in the peak numbers of the target FP (there is no duplication, or the peak numbers are aligned in the descending order), the process of Steps S404 to S407 is repeated.
In Step S408, a process of “storing an assignment result (TEMP)” is performed. In this process, the peak numbers of all the peaks, the retention time points and the peaks of the reference FP and peak data of the target FP that is specified as the peaks corresponding to these peak of the reference FP are stored in TEMP, and it proceeds to Step S409.
In Step S409, a determining process “Are all the peaks of the target FP included in TEMP?” is performed. In this process, it is determined whether the peak data of all the peaks of the target FP is included in TEMP stored in Step S408. If all included (YES), it is determined that the process for all the peaks of the target FP has been completed, and it proceeds to Step S412. If there is any excluded peak (NO), in order to add peak data of the excluded peak, it proceeds to Step S410.
In Step S410, a process of “correcting the retention time point of the peak of the target FP that is not included in TEMP” is performed. In this process, the retention time point of the peak of the target FP (the peak of the target FP that is needed to be corrected) that is excluded from TEMP is corrected as a correction value=k1+(k2−k1)*(t0−t1)/(t2−t1), wherein:
k1: it is a retention time point of a peak having a shorter retention time point of two reference FP-side peaks that are assigned in the vicinity of a peak of a target FP for which correction is necessary;
k2: it is a retention time point of a peak having a larger retention time point of two reference FP-side peaks that are assigned in the vicinity of the peak of the target FP for which correction is necessary;
t0: it is a retention time point of the peak of the target FP for which correction is necessary;
t1: it is a retention time point of a peak having a shorter retention time point of two target FP-side peaks that are assigned in the vicinity of the peak of the target FP for which correction is necessary; and
t2: it is a retention time point of a peak having a longer retention time point of two target FP-side peaks that are assigned in the vicinity of the peak of the target FP for which correction is necessary, and it proceeds to Step S411.
In Step S411, a process of “adding the corrected retention time point and the peak data thereof to TEMP, and updating TEMP” is performed. In this process, the retention time point of the peak of the target FP corrected in S410 and not included in TEMP is compared with the retention time points of the reference FP in TEMP, to add the corrected retention time point and peak data of the peak of the target FP that is not included in TEMP to a valid position in TEMP and update TEMP, and it proceeds to Step S409. Until all the peaks of the target FP are added, the process of Steps S409 to S411 is repeated.
In Step S412, a process of “outputting TEMP to a collation result file” is performed. In this process, TEMP that specifies the correspondence relation between all the peaks of the reference FP and the all the peaks of the target FP is output as a collation result file, and the target FP assigning process 3 ends.
In Step S501, a process of “reading the collation result file” is performed. In this process, the collation result file output in Step S412 illustrated in
In Step S502, a process of “reading the reference group FP” is performed. In this process, the reference group FP that is a final assignment opponent of each peak of the target FP is read, and it proceeds to Step S503.
In Step S503, a process of “integrating and storing the target FP and the reference group FP (TEMP)” is performed. In this process, two files are integrated based on the peak data of the reference FP that is commonly present in the collation result file and the reference group FP to store the result as TEMP, and it proceeds to Step S504.
In Step S504, a process of “correcting the retention time point of the peak of the target FP that does not correspond to any peaks in the reference FP” is performed. In this process, the retention time points of all the peaks of the target FP that do not correspond to any peaks in the reference FP in the collation result file are corrected to the retention time points of TEMP stored in Step S503, and it proceeds to Step S505. In addition, the correction for the retention time point is performed by the same method as that of Step S410 of the “Target FP Assigning Process 3”.
In Step S505, a process of “sequentially acquiring the peak data (P1) corresponding to the corrected retention time point (R1 and R3)” is performed. In this process, peak data pieces of peaks corresponding to as retention time points corrected in Step S504 as R1 and R3 are sequentially acquired as P1, and it proceeds to Step S506.
In Step S506, a process of “sequentially acquiring peak data (P2) of the target FP corresponding to retention time point (R2) of assignment candidate peak from TEMP” is performed. In this process, peak data pieces are sequentially acquired as P2 corresponding to retention time points R2 at which no peak of the target FP are assigned from TEMP stored in Step S503, and it proceeds to Step S507.
In Step S507, a determining process “|R1−R2|<threshold value 1?” is performed. In this process, it is determined whether or not a difference between the retention time points R1 and R2 acquired in Steps S505 and S506 is less than the threshold value 1. If a difference is less than the threshold value (YES), it is determined that there is a possibility that the retention time point of the target FP with the retention time point R1 corresponding to the retention time point of the reference FP with the retention time point R2, and it proceeds to Step S508. If a difference between the retention time points R1 and R2 is “1” or more (NO), it is determined that there is no possibility of the correspondence, and it proceeds to Step S512.
In Step S508, a process of “acquiring UV spectra (U1, U2) corresponding to the retention times R1 and R2” is performed. In this process, the UV spectra corresponding to the peaks of the retention time points of R1 and R2 that are determined to have the possibility of the correspondence in Step S507 are acquired from respective FPs, and it proceeds to Step S509.
In Step S509, a process of “calculating the degree of matching between the UV spectra (UV_Sim)” is performed. In this process, the UV_Sim is calculated using the same method as that of Step S306 of the “Target FP Assigning Process 2” of Step S3 based on the UV spectra U1 and U2 acquired in Step S508, and it proceeds to Step S510. In addition, a detailed calculation flow of the UV_Sim will be additionally described with reference to Subroutine 2 illustrated in
In Step S510, a determining process “UV_Sim<threshold value 2?” is performed. In this process, it is determined whether the UV_Sim calculated in Step S509 is less than the threshold value 2. If it is less than the threshold value 2 (YES), it is determined that the peak of the UV spectrum U1 corresponds to the peak of U2, and it proceeds to Step S511. If the UV_Sim is the threshold value 2 or more (NO), it is determined that there is no correspondence, and it proceeds to Step S507.
In Step S511, a process of “R3←R2, and threshold value 2←UV_Sim” is performed. In this process, the retention time point R3 (that is, R1) determined to have the correspondence in Step S510 is updated with R2 that is the retention time point of the corresponding opponent, and thereafter, the threshold value 2 is updated with the value of UV_Sim, and it proceeds to Step S507.
In Step S512, a determining process “Have the retention time points of all the assignment candidate peaks been compared?” is performed. In this process, it is determined whether comparisons of R1 with the retention time points of all the assignment candidate peaks have been completed. If completed (YES), it proceeds to Step S513. If not completed (NO), it proceeds to Step S507.
In Step S513, a process of “storing R1, R3 and P1 as well as the threshold value 2 (TEMP2)” is performed. In this process, the retention time point (R1) determined to have the correspondence in Step S510 and the peak (P1) corresponding to R3 updated to the retention time point (R2) of the corresponding opponent are stored as well as the threshold value 2 at this time (TEMP2), and it proceeds to Step S507.
In Step S514, a determining process “Have the retention time points of all non-corresponding peaks been compared?” is performed. In this process, it is determined whether or not comparisons with the retention time points of the assignment candidate peaks have been completed in the retention time points of all non-corresponding peaks. If completed (YES), it is determined that the assignment process of all the non-corresponding peaks has been completed, and it proceeds to Step S516. If not completed (NO), it is determined that one or more non-corresponding peaks that have not been processed remain, and it proceeds to Step S515.
In Step S515, a process of “threshold value 2+→initial value” is performed. In this process, the threshold value 2 that is updated to UV_Sim in Step S511 is returned to the initial value, and it proceeds to Step S505.
In Step S516, a determining process “Are there peaks having the same value of R3 present in TEMP2?” is performed. In this process, it is determined whether or not a plurality of non-corresponding peaks are assigned to the same peak in TEMP. If there are non-corresponding peaks assigned to the same peak (YES), it proceeds to Step S517. If such non-corresponding peak is not present (NO), it proceeds to Step S518.
In Step S517, a process of “comparing the threshold values 2 of the peaks having the same values of R3 and returning R3 of the peak having a larger threshold value to its original value (R1) “is performed. In this process, the threshold values 2 of the peaks having the same value of R3 in TEMP2 are compared with each other, to return the value of R3 of the peak having a larger threshold value to its original value (in other words, R1), and it proceeds to Step S518.
In Step S518, a process of “adding a peak of TEMP2 to TEMP (only a peak of whose R3 coincides with the retention time point of TEMP)” is performed. In this process, every peak of which R3 coincides with the retention time point of TEMP is added to TEMP, and it proceeds to Step S519. Every peak of which R3 does not coincide with the retention time point of TEMP is not added, because there is no peak to be an assignment opponent in the reference group FP.
In Step S519, a process of “outputting the peaks of the target FP included in TEMP (peak feature value file)” is performed. In this process, the peak data of the target FP assigned to the reference group FP 137 is output as a peak data feature value file, to finish the target FP assigning process 4.
In Step S1001, a process of “x←R1 and y←R2” is performed. In this process, R1 and R2 acquired in Steps S202 and S206 of
In Step S1002, a process of “acquiring the numbers of data “x” and “y” (a, b)” is performed. In this process, the numbers of data “x” and “y” are acquired as “a” and “b,” respectively, and it proceeds to Steps S1003.
In Step S1003, as an initial value of a counter used for sequentially invoking the retention time points of “x”, “1” is substituted into “i” (i←1), and it proceeds to Step S1004.
In Step S1004, a process of “acquiring all distances from the xi-th retention time point (f)” is performed. In this process, all distances, from the xi-th retention time point, of retention time points after the xi-th retention time point are acquired as “f”, and it proceeds to Step S1005.
In Step S1005, as an initial value of a counter for sequentially invoking the retention time points of “y”, “1” is substituted into “j” (j←1), and it proceeds to Step S1006.
In Step S1006, a process of “acquiring all distances from the yj-th retention time point (g)” is performed. In this process, all distances, from the yj-th retention time point, of retention time points after the yj-th retention time point are acquired as “g”, and it proceeds to Step S1007.
In Step S1007, a process of “acquiring the number of data pieces satisfying a relation of “|inter-retention time point distance of “f”—inter-retention time point distance of “g”|<threshold value” (m)” is performed. In this process, an inter-retention time point distances “f” and “g” acquired in Steps S1004 and S1006 are compared with each other in a round robin manner, the number of data pieces satisfying the condition of “|inter-retention time point distance of “f”—inter retention time point distance of “g”|<threshold value” is acquired as “m”, and it proceeds to Step S1008.
In Step S1008, a process of “calculating the degree of matching between the retention time appearance patterns of “f” and “g” (RPfg)” is performed. In this process, RPfg is calculated based on “a” and “b” acquired in Step S1002 and “m” acquired in Step S1007 as:
RP
fg=(1−(m/(a+b−m)))×(a−m+1).
It proceeds to Step S1009.
In Step S1009, a process of “storing RPfg (RP_all)” is performed. In this process, the degree of matching calculated in Step S1008 is stored in RP_all, and it proceeds to Step S1010.
In Step S1010, a process of “updating j (j←j+1)” is performed. In this process, in order to perform the process of “y” at the next retention time point, “j+1” is substituted into “j” as the update of “j”, and it proceeds to Step S1011.
In Step S1011, a determining process “Has the process been completed at all the retention time points of “y”?” is performed. In this process, it is determined whether or not the process of all the retention time points of “y” has been completed. If completed (YES), it is determined that the process of all the retention time points has been completed, to proceed to Step S1012. If not completed (NO), it is determined that one or more retention time points that have not been processed remain in “y”, to proceed to Step S1006. In other words, the process of Steps S1006 to S1011 is repeated until all the retention time points of “y” is processed.
In Step S1012, a process of “updating “i” (i←i+1)” is performed. In this process, as the update of “i” for bringing the process of “x” to the next retention time point. “i+1” is substituted into “i”, and it proceeds to Step S1013.
In Step S1013, a determining process “Has the process been completed at all the retention time points of “x”?” is performed. In this process, it is determined whether or not the process of all the retention time points of “x” has been completed. If completed (YES), it is determined that the process of all the retention time points of “x” has been completed, to proceed to Step S1014. If not completed (NO), it is determined that one or more retention time points that have not been processed remain in “x”, to proceed to Step S1004. In other words, the process of Steps S1004 to S1013 is repeated until all the retention time points of “x2 are processed.
In Step S1014, a process of “acquiring a minimum value from RP_all (RP_min)” is performed. In this process, the minimum value in RP_all in which RPs for all the combinations of the retention time appearance patterns of the target FP and the reference FP are stored is acquired as RP_min, and RP_min is input to Step S207 of
In Step S2001, a process of “x←U1, y←U2, z←0” is performed. In this process, the UV spectra U1 and U2 acquired in Steps S302 and S304 of
In Step S2002, a process of “acquiring the number of data pieces of “x” (a)” is performed. In this process, the number of data pieces of “x” is acquired as “a”, and it proceeds to Step S2003.
In Step S2003, a process of “i←1” is performed. In this process. “1” is substituted into “i” as an initial value used for sequentially invoking absorbance at each detection wavelength configuring the UV spectra U1 and U2 from “x” and “y”, and it proceeds to Step S2004.
In Step S2004, a process of “acquiring the xi-th data (b)” is performed. In this process, the i-th absorbance data of “x” into which the UV spectrum “U1” is substituted is acquired as “b”, and it proceeds to Step S2005.
In Step S2005, a process of “acquiring yi-th data (c)” is performed. In this process, the i-th absorbance data of “y” into which UV spectrum U2 is substituted is acquired as “c”, and it proceeds to Step S2006.
In Step S2006, a process of “calculating an inter-UV spectra distance (d) and a sum (z) of squares of the inter-UV spectra distance” is performed. In this process, the inter-UV spectra distance “d” and the sum “z” of squares of the inter-UV spectra distance are calculated as:
d=b−c; and
z=z+d
2.
It proceeds to Step S2007.
In Step S2007, a process of “updating i (i←i+1)” is performed. In this process, as the update of “i,” “i+1” is substituted into “i,” to proceed to Step S2008.
In Step S2008, a determining process “Have the process of all data of “x” been completed ?” is performed. In this process, it is determined whether the process of all data of “x” and “y” have been completed. If completed (YES), it is determined that the process of all data of “x” and “y” have been completed, to proceed to Step S2009. If not completed (NO), it is determined that there are one or more data pieces of “x” and “y” that have not been processed, to proceed to Step S2004. In other words, the process of Steps S2004 to S2008 is repeated until all the absorbance data of “x” and “y” is processed.
In Step S2009, a process of “calculating the degree of matching between the UV spectra of “x” and “y” (UV_Sim)” is performed. In this process, UV_Sim is calculated based on the sum “z” of squares of the inter-U V spectra distance and the number “a” of data of “x” as follows:
UV
—
Sim=√(z/a).
UV_Sim is input to Step S306 of
In Step S3001, a process of “setting the number (m) of peak pattern configuring candidates and the number (n) of peak pattern configuring peaks” is performed. In this process, as setting for comprehensively preparing peak patterns, the number (m) of peak pattern configuring candidates and the number (n) of peak pattern configuring peaks are set, and it proceeds to Step S3002.
In Step S3002, a process of “x←target FP name, r1←R1, p1←P1, y←reference FP name, r2←R2, and p2←P2” is performed. In this process, the file names of the target FP and the reference FP that are necessary for the process, and the retention time points and the peak data acquired in Steps S302 and S304 of
In Step S3003, a process of “acquiring all retention time points of “x” (a)” is performed. In this process, a file (target FP) having a name substituted into “x” in Step S3002 is read, all the retention time points of the file are acquired as “a”, and it proceeds to Step S3004.
In Step S3004, a process of “acquiring all retention time points of “y” (b)” is performed. In this process, a file (reference FP) having a name substituted into “y” in Step S3002 is read, all the retention time points of the file are acquired as “b”, and it proceeds to Step S3005.
In Step S3005, a process of “acquiring retention time points (cm) and peak data (dm) of m peak pattern configuring candidate peaks of “r1” from “a”” is performed. In this process, retention time points of m peak pattern configuring candidate peaks of “r1” that are the retention time points of the assignment target peaks are acquired as “cm” and the peak data thereof as “dm” from “a”, and it proceeds to Step S3006. Here, m peak pattern configuring candidate peaks are m peaks with retention time points close to “r1.”
In Step S3006, a process of “acquiring retention time points (em) and peak date (fm) of m peak pattern configuring candidate peaks of “r2” from “b”” is performed. In this process, retention time points of m peak pattern configuring candidate peaks of “r2” that are the retention time points of the assignment target peaks are acquired as “em” and the peak data thereof as “fm” from “b”, and it proceeds to Step S3007. Here, m peak pattern configuring candidate peaks are m peaks with retention time points close to “r2”.
In Step S3007, a process of “aligning “cm” and “dm” in the retention time order (ascending order)” is performed. In this process, “cm” and “dm” acquired in Step S3005 are rearranged so as to be in the ascending order of the retention time, and it proceeds to Step S3008.
In Step S3008, a process of “aligning “em” and “fm” in the retention time order (ascending order)” is performed. In this process, “em” and “fm” acquired in Step S3006 are rearranged so as to be in the ascending order of the retention time, and it proceeds to Step S3009.
In Step S3009, a process of “sequentially acquiring retention time points (cn) and peak data (dn) of n peak pattern configuring peaks from “cm” and “dm”” is performed. In this process, retention time points are sequentially acquired as “cn” and the peak data thereof as “dn” from “cm” and “dm” of m peak pattern configuring candidate peaks, and it proceeds to Step S3010.
In Step S3010, a process of “sequentially acquiring retention time points (en) and peak data (fn) of n peak pattern configuring peaks from “em” and “fm”” is performed. In this process, retention time points of n peak pattern configuring peaks are sequentially acquired as “en” and the peak data thereof as “fn” from “em” and “fm” of m peak pattern configuring candidate peak, and it proceeds to Step S3011.
In Step S3011, a process of “calculating the degree of matching between peak patterns (P_Sim)” is performed. In this process, the degree (P_Sim) of matching between peak patterns is calculated based on “r1” and “p1” of the assignment target peaks, “cn” and “dn” of n peak pattern configuring peaks, “r2” and “p2” of the assignment candidate peaks, and “en” and “fn” of n peak pattern configuring peaks, which have been acquired until now as:
in the case of n=4 as an example as represented in
In Step S3012, a process of “storing P_Sim (P_Sim_all)” is performed. In this process, P_Sim calculated in Step S3011 is sequentially stored in P_Sim-all, and it proceeds to Step S3013.
In Step S3013, a determining process “Have all the combinations to take out n pieces from m pieces included in “em” been completed?” is performed. In this process, it is determined whether or not the process has been completed for all the combinations to take out n peak pattern configuration peaks out from m peak pattern configuring candidate peaks. If completed (YES), it is determined that the preparation of comprehensive peak patterns and the calculation of the degrees of matching for the patterns have been completed for the assignment candidate peaks, to proceed to Step S3014. If not completed (NO), it is determined that one or more combinations to take out n pieces out from m pieces have not been completed, to proceed to Step S3010. In other words, the process of Steps S3010 to S3013 is repeated until the process is completed for all the combinations to take out n pieces out from m pieces.
In Step S3014, a process of determining “Have all the combinations to take out m pieces from n pieces included in “cm” been completed?” is performed. In this process, it is determined whether or not the process has been completed for all the combinations to take out n peak pattern configuring peaks from m peak pattern configuring candidate peaks of the assignment target peak. If completed (YES), it is determined that the preparation of comprehensive peak patterns and the calculation of the degrees of matching for the patterns have been completed for the assignment candidate peak, to proceed to Step S3015. If not completed (NO), it is determined that one or more combinations to take out n pieces from m pieces has not been completed, to proceed to Step S3009. In other words, the process of Steps S3009 to S3014 is repeated until the process is completed for all the combinations to take out n pieces out from m pieces.
In Step S3015, a process of “acquiring a minimum value from P_Sim_all (P_Sim_min)” is performed. In this process, the minimum value of the P_Sim-all stored in S3012 is acquired as P_Sim_min, and the P_Sim_min is input to Step S307 of
The reference FP feature value file is prepared for comparing the target FP feature value data with the reference FP feature value data as illustrated in
The reference FP preparing function is realized in Step S10001. The reference FP peak assigning function is realized in Steps S10002, S10003, and S10004.
The reference FP assigning result integrating function is realized in Step S10005. The reference FP peak feature value preparing function is realized in Step S10006.
Steps S10001 to S10004 correspond to Steps S1 to S4 relating to the preparation of the target FP feature value integrating file illustrated in
In Step S10001, the “FP preparing process” is performed according to a 3D chromatogram and peak information at a specific detection wavelength as inputs.
Both the 3D chromatograph and the peak data are provided for each one of a plurality of evaluation reference drug (reference kampo medicine) that are evaluation criteria.
In Step S10001, the reference FP preparing part of the computer functions and a reference FP is prepared similarly to the target FP 17 (
In Step S10002, the “reference FP assigning process 1” is performed according to all reference FPs output in Step S10001 as inputs.
In Step S10002, the reference FP peak assigning part of the computer functions, and, for all the reference FPs, a combination is selected from among the all reference FPs in order to calculate assignment scores for the selected combination in the selected order, and it proceeds to Step S10003.
In Step S10003, the “reference FP assigning process 2” is performed according to the selected combination of the reference FPs as an input.
In Step S10003, for all the peaks of the combination of the reference FPs that is selected in Step S2, peak patterns are comprehensively prepared as illustrated in
In Step S10004, the “reference FP assigning process 3” is performed according to the determination result file output in Step S10003 as input.
In Step S10004, between the reference FPs in the selected combinations, peaks of the reference FPs in the selected combinations, which correspond to each other are specified based on the degree of matching between the assignment candidate peaks (SCORE). The result is output as the reference FP assigning data for each reference FP.
In Step S10005, the “reference FP assigning result integrating process” is performed according to all the reference FP assigning data output in Step S10004 is received as input.
In Step S10005, the reference FP assigning result integrating part of the computer functions to prepare a reference FP correspondence table by integrating all the FP assigning data with reference to the peak correspondence relation of the individual reference FP specified by the reference FP peak assigning part, and it proceeds to Step S10006.
In Step S10006, the reference FP peak feature value preparing part of the computer functions to prepare a peak feature value (reference group FP) according to the all reference FPs based on the reference FP correspondence table that is prepared by the reference FP assigning result integrating part. In the process at the reference FP peak feature value preparing part, statistic values (a maximum value, a minimum value, a medium value, an average value, and the like) are calculated for each peak (column) in the reference FP correspondence table, to select the peak (column) based on the calculated information. The selected peak (column) is output as the reference group FP (see the reference group FP example 137 illustrated in
In Step S10101, a process of “reading the 1st assignment data in the assignment order as integrated data” is performed. In this process, the reference FP assigning data, in which the assignment process is performed first to specify the correspondence relation of peaks in Step S10004, is read as the integrated data. Then, it proceeds to Step S10102.
In Step S10102, a process of “sequentially reading subsequent assignment data” is performed. In this process, at first the reference FP assigning data, in which the assignment process is secondarily performed to specify the correspondence relation of peaks in Step S10004, is read as integrated data. Then, it proceeds to Step S10103.
In Step S10103, a process of “integrating the integrated data and the assignment data with common peak data” is performed. In this process, the two files are integrated based on the peak data of the reference FP commonly-existing in the integrated data and the assignment data, the integrated data is updated as a result thereof, and it proceeds to Step S10104.
In Step S10104, a determining process “Have all the peaks included in the assignment data been added to the integrated data?” is performed. In this process, it is determined whether or not all the peaks in the assignment data have been added to the integrated data. If added (YES), it proceeds to Step S10105. If there is one or more peaks (lacking peaks) that have not been added (NO), in order to add the lacking peaks to the integrated data, it proceeds to Step S10107. In addition, in the process (S10107 to S10120) of adding the lacking peaks to the integrated data, the same process as that of Steps S504 to S517 in S5 (target FP assigning process 4) is performed.
In Step S10121, a process of “adding data of TEMP2 to the integrated data (all the retention time points and peaks)” is performed. In this process, all the retention time points (R3) and the peaks (P1) in TEMP2 are added to corresponding positions in the integrated data, and it proceeds to Step S10122.
In Step S10122, a process of “threshold value 2←initial value, and deleting all the data in TEMP2” is performed. In this process, the threshold value 2 updated to UV_Sim is returned to the original value, all the data are deleted from TEMP2 storing data such as retention time points and peaks of all the lacking peaks and the like, and it is returned to Step S10104.
In Step S10105 to which it proceeds from Step S10104, a determining process “Has the process of all the assignment data been completed?” is performed. In this process, it is determined whether or not the process of all reference data has been completed. If completed (YES), in order to output a reference FP correspondence table that is an integration result of all the assignment data, it proceeds to Step S10106. If not completed (NO), it is returned to Step S10102 to sequentially process the remaining assignment data.
In Step S10106, a process of “outputting the integrated data (reference FP correspondence table)” is performed. In this process, the result integrating all the assignment data is output as the reference FP correspondence table, to finish the process of preparing the reference FP correspondence table.
In Step S10201, a process of “reading the reference FP correspondence table” is performed. In this process, the reference FP correspondence table prepared in Step S10005 is read to proceed to Step S10202.
In Step S10202, a process of “calculating statistic values for each peak (column)” is performed. In this process, the statistic values (a maximum value, a minimum value, a medium value, an average value, a variance, a standard deviation, an existence number, and an existence ratio) are calculated for each peak (column) of the reference FP correspondence table. Then, it proceeds to Step S10203.
In Step S10203, a process of “selecting a peak (column) with reference to the calculated statistic values” is performed. In this process, a peak is selected with reference to the statistic values calculated in Step S10102, to proceeds to Step S10204.
In Step S10204, a process of “outputting the selected peak (column) (reference group FP)” is performed. In this process, the selecting result of the peak (column) according to the statistic amounts is output as the reference group FP to finish of preparing the reference group FP.
In Embodiment 1 of the present invention, the FP preparing step 113 preparing target FP 17 that comprises peaks, retention time points and UV spectra of the peaks detected from the 3D chromatogram 15 of the multicomponent drug that is the evaluation target at a specific wavelength, for example, 203 nm; the reference FP selecting step 115 selecting a reference FP that is appropriate to peak assignment of the target FP 17 from among a plurality of reference FPs; a peak pattern preparing step 117 preparing peak patterns that comprises, for example, three peaks including two peaks that are present at least on one of sides located in front and in the rear in a time axis direction for each peak of the target FP and the selected reference FP; the peak assigning step 119 comparing the peak patterns and the UV spectra of the peaks to specify corresponding peaks; and the evaluating step 121 evaluating the assigned peak of the target FP by comparison with the peaks of the plurality of reference FPs, for example, with use of MT method.
By processing the 3D chromatogram 15 of the multicomponent drug that is an evaluation target through these five steps (113, 115, 117, 119, and 121), it can improve the accuracy and the efficiency of the quality evaluation of the evaluation target drug.
The target FP 17 prepared by the FP preparing step 113, similarly to the 3D chromatogram 15, is configured as three dimensional information (peaks, retention time points, and UV spectra). Accordingly, the target FP 17 is data directly succeeding to the information unique to the drug. In spite of that, the data volume is compressed at the ratio of about 1/70, compared to the 3D chromatogram 15, the amount of information to be processed can be greatly reduced to increase the processing speed.
The FP preparing step 113 prepares a FP by composing a plurality of FPs at different detection wavelengths. Accordingly, for even a multicomponent drug acquired by combining components all of which cannot be detected using one wavelength, a quality evaluation including all the components can be performed by composing a FP having a plurality of detection wavelengths.
The FP preparing step 113 prepares a FP that includes all the peaks detected in the 3D chromatogram. Accordingly, the FP preparing step is suited for an evaluation of the quality of a kampo medicine that is a multicomponent drug.
The reference FP selecting step 115 compares retention time appearance patterns of FPs with each other, to select a reference FP having a high degree of matching between patterns as a reference FP that is appropriate to the assignment. Accordingly, in the peak assigning step 119, the assignment process can be performed between FPs having similar patterns, whereby assignment can be performed with high accuracy.
The peak pattern preparing step 117 comprehensively prepares peak patterns with use of a plurality of peripheral peaks for each of the assignment target peak and the assignment candidate peak. Accordingly, even if there is a difference between the whole patterns of the target FP and the reference FP more or less, assignment can be performed through the peak assigning step 119 with high accuracy.
The peak assigning step 119, in addition to the degree of matching between peak patterns prepared by the peak pattern preparing step 117, the degree of matching between UV spectra of the assignment target peak and the assignment candidate peak is used for specifying the peak to be assigned. Accordingly, assignment can be performed with high accuracy.
The peak assigning step 119 assigns all the peaks of the target FP to the peaks of the reference FP all together. Accordingly, the assignment process can be performed with high efficiency.
The evaluating step 121 collects a FP that is composed by multiple components as multi-dimensional data as a MD value in one dimension by MT method, to easily compare and evaluate a plurality of evaluation target lots. Accordingly, it is suited for evaluating a multicomponent based drug that is composed of multiple components.
The evaluating program for a multicomponent drug according to this embodiment of the present invention realizes the functions in a computer to improve the accuracy and the efficiency of the evaluation.
The evaluating apparatus for a multicomponent drug according to this embodiment of the present invention operates the units 3, 5, 7, 9 and 11 to improve the accuracy and the efficiency of the evaluation.
Modified Examples of Calculation of Degree of Matching between Peak Patterns (P_Sim)
In the case of
In the FP preparing method, an FP preparing program, an FP preparing device, and an FP, there may be a case where a peak represents a maximum value of a signal strength (height) as described above or a case where a peak represents an area value (peak area) of a signal strength in a form of a height.
In other words, even in the case where the FP is prepared with use of peak areas, the area values are represented in a form of heights to prepare the FP. Accordingly, the FP has the same representation as that of the case where the FP is prepared with use of the peak heights as in the above-described embodiment. Therefore, similar to the case where the FP is prepared with use of the peak heights, the FP can be evaluated by the process of the above-described embodiment.
However, in the case where the FP is prepared with use of the peak areas, differences between the peak values of comparison targets are larger. Accordingly, it is appropriate that the calculation is made based on a ratio so as to make the handling thereof easy.
Hereinafter, the degree of matching between peak patterns (P_Sim) that is calculated based on the ratios will be represented for exemplary cases where n=2 and n=4.
In the case where n=2, the calculation is represented as follows:
In a case where n=4, the calculation is represented as follows:
Here, #1 represents a ratio (larger value/smaller value) of two comparison target values.
In addition, also in the case where the FP is prepared by means of the peak heights, the degree of matching between peak patterns (P_Sim) can be calculated based on a ratio, and, also in the case where the FP is prepared by means of the peak areas, similarly to the case of a difference between the peak heights, the degree of matching between peak patterns (P_Sim) can be acquired based on a difference between peak area values.
In the modified example of this Subroutine 2, a process of adding inclination information in moving average of a UV pattern (DNS) to the RMSD of Subroutine 2 in
By adding the DNS information to the RMSD, the degree of matching between waveforms of UV spectra can be calculated more accurately.
In Subroutine 2 according to the modified example of
In Subroutine 2 of this modified example, Steps S2010 to S2013 are added so as to add the DNS, so that it enables Steps S2009A to calculate the degree of matching to which the DNS is added.
In Step S2010, a determining process of “Is the DNS added?” is performed. If the DNS is determined to be added (YES), it proceeds to Step S2011. If the DNS is determined not to be added (NO), it proceeds to Step S2009A. The determination whether the DNS is added or not is based on, for example, an initial setting. For example, if the FP is prepared by means of peak areas, the DNS is set to be added; and if the FP is prepared by means of peak heights, the DNS is set to be not added.
However, also in the case of the above-described embodiment in which the FP is prepared by means of peak heights, the degree of matching between UV patterns can be calculated through a process to which the DNS is added; and also in the case where the FP is prepared by means of peak areas, the degree of matching between UV patterns can be calculated through the process of the above-described embodiment to which the DNS is not added.
In Step S2011, a process of “calculating the moving averages of “x” and “y” in interval 1 (w1)” is performed, to find the moving averages for interval 1 (w1). Interval 1 (w1) is an interval relating to the wavelength of the UV data. In a case where w1=3 in the initial setting of Step S2001, interval 1 (3) is set and the average of the UV intensities of three wavelengths is acquired. More specifically, description will be made later with reference to a table represented in
In Step S2012, the process of “calculating the moving inclinations of “x” and “y” in interval 2 (w2)” is performed to acquire the moving inclinations in interval 2 (w2). Interval 2 (w2) is an interval relating to the moving average acquired in Step S2011. If w2=3 in the initial setting performed in Step S2001, interval 2 (3) is set to acquire inclinations of (±) over the three moving averages based on the moving averages calculated in Step S2011. More specifically, description will be made later with reference to a table illustrated in
In Step S2013, a process of “calculating the number of mismatches between the codes of the moving inclinations of “x” and “y” (DNS)” is performed, to calculate the number of matches in the inclinations of (±) based on the moving inclinations calculated in Step S2012. The moving inclination of (+) represents rising to the right in
When proceeding from Step S2013 to Step S2009A, the degree of matching to which the DNS is added is calculated in the process of Step S2009A.
In Step S2009A, a process of “calculating the degree of matching between UV spectra of “x” and “y” (UV_Sim)” is performed. In the calculation process of the degree of matching to which the DNS is added, the UV_Sim is calculated based on the sum “z” of squares of inter-UV spectrum, the number “a” of data of “x” and the DNS distances as:
UV
—
Sim=√(z/a)×1.1DNS.
This UV_Sim is input to Step S306 in
In addition, the process performed in a case where it proceeds from Step S2010 to Step S2009A is the same as that of Step S2009 in
In
For the example of the interval 1 (w1=3), the moving averages are calculated as m1, m2 . . . as respective values calculated for an interval (a1, a2, a3), an interval (a2, a3, a4) . . . in Step S2012 (see
In this way, when preparing the FP by means of peak areas, in the assignment process to the reference group FP and the reference FP assigning result integrating process, the degree of matching between UV patterns can be calculated through the process to which the DNS is added. With this calculation, even if a distance (dis) between two corresponding points illustrated in
Although this embodiment of the present invention is applied to an evaluation of a kampo medicine as a multicomponent drug, it can be also applied to an evaluation of other multicomponent materials.
Although all the peaks on the 3D chromatogram are set as targets in the FP of the embodiment, the FP may be prepared with the exclusion of fine data such as peaks each having a peak area corresponding to 5% or less on the 3D chromatogram.
In the above-described embodiment, the FP is prepared based on the peak heights, to acquire evaluations in
| Number | Date | Country | Kind |
|---|---|---|---|
| 2011-123845 | Jun 2011 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP2012/003607 | 5/31/2012 | WO | 00 | 2/6/2013 |
