Claims
- 1. A reagent system for the determination of fructosamine in sera which comprises:
- (a) a first liquid alkaline aqueous reagent containing a tetrazolium salt which reacts with all reactive substances in sera including fructosamine and interfering substances to exhibit a measurable first change of color within a predetermined time span of less than about 10 minutes in consequence of the reaction of the tetrazolium salt with fructosamine and the interfering substances; and
- (b) a second alkaline aqueous reagent identical to the first alkaline aqueous reagent but including a substance which inhibits reaction of fructosamine with the tetrazolium salt by binding or complexing to the fructosamine to produce by reduction of the tetrazolium salt with the reactive substances including interfering substances other than fructosamine a measurable second change of color in the same predetermined time span as the first reagent to enable the determination of fructosamine concentration in sera by the difference in the first color change produced using the first alkaline aqueous reagent which reacts with fructosamine and the second color change produced using the second alkaline aqueous reagent, each color change produced on the same proportional amount of sera and after identical predetermined time spans.
- 2. The reagent system as claimed in claim 1 in combination with a liquid standard containing a predetermined amount of fructosamine which when used with said first and second alkaline aqueous reagents enable the determination of a factor used as multiplier used to compute from the difference in color change the fructosamine concentration in a sera sample.
- 3. An assay system as claimed in claim 2 further including a liquid normal level fructosamine control and a liquid high level fructosamine control.
- 4. An assay system as claimed in claim 2 further including a liquid normal level fructosamine control having a fructosamine range of 229-281 .mu.mol, when assayed, and a liquid high fructosamine level control having a fructosamine range, when assayed, of 457-559 .mu.mol.
- 5. An assay system as claimed in claim 3 further including a liquid normal fructosamine level control having a fructosamine range of 229-281 .mu.mol, when assayed, and a liquid high fructosamine level control having a fructosamine range, when assayed, of 457-559 .mu.mol.
- 6. A method for determining fructosamine in sera which comprises:
- (a) determining by absorbance a first color produced by reaction of a first amount of sera, with a first amount of a liquid alkaline aqueous reagent to produce a first change in color in consequence of reduction by fructosamine and other interfering substances in sera of a tetrazolium salt at two predetermined points in time, each within 10 minutes at 37.degree. C.;
- (b) determining absorbance of a second color produced by reaction of the same amount of reagent with the same amount of said sera using a second liquid alkaline aqueous reagent which produces a second color change by reaction of the same amount of said tetrazolium salt with interfering substances in the sera other than fructosamine in consequence of the reagent containing a substance which inhibits the reaction of fructosamine with the color producing tetrazolium salt, by binding or complexing to the fructosamine the change in absorbance being determined for the same predetermined period of time; and
- (c) determining the fructosamine concentration in the sera as function of the difference in the first and second color change produce using the first liquid alkaline aqueous reagent which reacts with fructosamine and the second liquid alkaline aqueous reagent which is inhibited from reacting with fructosamine.
Parent Case Info
This is a continuation division of application Ser. No. 07/895,606 filed Jun. 8, 1992 now U.S. Pat. No. 5,312,760.
US Referenced Citations (4)
Continuations (1)
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Number |
Date |
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Parent |
895606 |
Jun 1992 |
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