Patent Application
20220296665
This application claims the benefit of priority based on Korean Patent Application No. 10-2019-0103194 filed on Aug. 22, 2019, the entire contents of which are incorporated herein as part of the present specification.
The present invention relates to a functional food composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
Irritable bowel syndrome (IBS) is a chronic functional disorder characterized by lower abdominal bloating or discomfort, abdominal pain, frequent passage of stools, diarrhea or constipation or their alternating phenomenon, flatus, unpleasant odor, and the like, and is a clinical symptom characterized by non-specificity and diversity and the absence of anatomical or organic lesion abnormalities.
Irritable bowel syndrome is defined somewhat differently depending on the researcher, and according to Rome criteria II, which reflects recent research results on functional gastrointestinal diseases, it has been defined as a group including several functional bowel diseases in which abdominal discomfort or pain is associated with defecation or which are accompanied by changes in bowel habits or abnormal defecation features. According to the data reported by Manning et al. in 1978, four symptoms were statistically significantly common in patients with irritable bowel syndrome: abdominal pain improved by stools, loose stools when symptoms occurred, increased frequency of passage of stools when pain occurred, and abdominal bloating; and the two symptoms of mucous stool and feeling of incomplete evacuation also tended to be found more frequently in patients with irritable bowel syndrome.
For the diagnosis of irritable bowel syndrome symptoms, experts from various countries gathered in 1989 and presented the diagnostic criteria of Rome criteria I for various functional gastrointestinal diseases, and the irritable bowel syndrome was diagnosed when abdominal pain or abdominal discomfort accompanied by changes in the frequency of passage of stools or the shape of stools persists for more than 3 months, and two or more of the five defecation disorders such as (1) change in the frequency of passage of stools, (2) change in stool form, (3) abnormal stool evacuation, (4) mucous stool and (5) abdominal bloating are accompanied. These diagnostic criteria have been revised and complemented over the years, and Rome criteria II in 1999 and Rome criteria III in 2006 were revised. Currently, the most widely used criteria is Roman criteria II, and in the diagnostic method, irritable bowel syndrome was diagnosed when symptoms start at least 6 months before and the abdominal pain or abdominal discomfort due to defecation is alleviated for at least 3 days every month for the past 3 months, or when there is a change in frequency and shape. It is also classified into diarrhea type, constipation type, and alternating diarrhea and constipation type, which are the subtypes of irritable bowel syndrome.
Although the cause of irritable bowel syndrome is not clearly known, it is generally expected to occur due to complex causes such as changes in intestinal motility, visceral hypersensitivity, psychosocial factors, neurotransmitter imbalance, bacterial infection and inflammation. In addition, although the exact mechanism of irritable bowel syndrome has not been elucidated, according to recently reported data, a correlation with the concentration of serotonin among neurotransmitters has been revealed, and in particular, it is known that the concentration of serotonin in the blood is mainly influenced by the concentration of serotonin in the intestinal tract, which affects the diarrhea-dominant type of irritable bowel syndrome. Other mechanisms include a study on visceral stimulation derived from the gastrointestinal wall, a mechanism according to the interaction changed due to low-grade inflammation between mucosal immune system and afferent nerve endings distributed in the intestine, oxidative stress, and the like.
In particular, it has been reported that oxidative stress that causes DNA damage increases the ulceration of the intestinal mucosa, and in this case, 8-hydroxy-2′-deoxyguanosine (8-OHdG) is used as a mainly used biomarker. It has been reported that intestinal mucosa damage affects the diarrhea-predominant type symptom of irritable bowel syndrome.
Since the main cause of such irritable bowel syndrome is weak intestine or stress, the fundamental effect cannot be achieved by simply treating symptoms such as constipation and diarrhea, but it is expected that the fundamental effect will be achieved only by relieving stress. In addition, in order to develop a food or therapeutic agent capable of obtaining such an effect, studies to obtain an active ingredient from a natural product with no or fewer side effects are being actively conducted, but no significant results have been reported yet.
Recently, as research results of a Lonicera japonica extract among natural products on anti-inflammatory, antibacterial, antipyretic, hepatoprotective, anticancer, immune, anti-allergic, lipid metabolism, anti-arthritis and sedative effects have been published, interest in food or pharmaceutical uses of a Lonicera japonica extract is growing.
Lonicera japonica refers to dried flower buds or freshly blooming flowers of Lonicera japonica Thunberg belonging to the family Caprifoliaceae. Since ancient times, all parts such as stems, leaves, roots and flowers of Lonicera japonica have been used as medicinal herbs. In particular, it is recorded in the Chinese Pharmacopoeia as one of the drugs that has been used in traditional Chinese medicine (TCM) for thousands of years, and it is known in China that it has superior efficacy than ginseng. The flowers of Lonicera japonica are very fragrant and contain a lot of honey, and have a mild and slightly bitter taste. Lonicera japonica has been traditionally used for headaches, acute fever, sore throat, infectious diseases and the like, and through recent studies, various research results such as anti-inflammatory, antibacterial, antipyretic, hepatoprotective, anticancer, immune, anti-allergic, lipid metabolism, anti-arthritis and sedative effects have been reported.
Korean Patent No. 10-1605312 relates to a food composition for the treatment or prevention of gastrointestinal motility disorder using Lonicera japonica extract. Specifically, it discloses that the acetate fraction of the Lonicera japonica water extract is effective in improving the symptoms of gastrointestinal motility disorder, which is one or more of functional dyspepsia, constipation, irritable bowel symptoms, diabetic gastrointestinal motility disorder, gastrointestinal motility disorder caused by chemotherapy, intestinal atresia caused by gastrointestinal motility disorder, and gastrointestinal motility disorder caused by myotonic dystrophy. The above patent suggests the use of a Lonicera japonica extract for improving gastrointestinal motility disorders, but there is a problem that only mentions symptoms related to gastrointestinal motility and does not provide a solution for improving various symptoms occurring in the gastrointestinal tract.
As such, studies on food or drugs for improving symptoms of various gastrointestinal disorders in relation to a Lonicera japonica extract are continuing, but no specific research has been done on food or drugs related to the improvement of irritable bowel syndrome with diarrhea.
[Prior Art Reference]
(Patent Document 1) Korean Patent No. 10-1605312
Accordingly, it is an object of the present invention to provide a functional food composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
It is another object of the present invention to provide a pharmaceutical composition for preventing or treating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
In order to achieve the above objects, the present invention provides a functional food composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
The present invention also provides a pharmaceutical composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
According to the present invention, the Lonicera japonica extract exhibits an effect of alleviating the symptoms of irritable bowel syndrome by improving the symptoms of diarrhea. Specifically, the Lonicera japonica extract may alleviate irritable bowel syndrome by exhibiting an effect of improving increased passage of stools, loose stools, and urgent need for defecation.
In addition, the Lonicera japonica extract according to the present invention exhibits an effect of alleviating irritable bowel syndrome by improving the borborygmus, which is one of the symptoms related to irritable bowel syndrome.
In addition, the Lonicera japonica extract exhibits an effect of alleviating irritable bowel syndrome by reducing the blood concentration of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is used as a biomarker for oxidative stress.
In addition, among the Lonicera japonica extracts, a Lonicera japonica water extract may be more effective in alleviating borborygmus and/or irritable bowel syndrome with diarrhea.
Hereinafter, the present invention will be described in more detail.
Food Composition
The present invention relates to a functional food composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient.
Lonicera japonica is a flower of Lonicera japonica Thunberg belonging to the family Caprifoliaceae, and is also called honeysuckle or Lonicera japonica Thunberg flower.
The Lonicera japonica extract may be extracted with a solvent selected from water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
The Lonicera japonica extract may be an organic solvent fraction of the Lonicera japonica extract. In this case, the organic solvent fraction of the Lonicera japonica extract refers to a substance obtained by independently or sequentially performing systematic fractionation of the Lonicera japonica extract using one or more of the following organic solvents.
The organic solvent may include one or more selected from the group consisting of hexane, ethyl acetate, dichloromethane, chloroform, and butanol.
For example, in consideration of the efficacy of alleviating irritable bowel syndrome, the organic solvent fraction of the Lonicera japonica extract may be an ethyl acetate fraction of the Lonicera japonica water extract as described below.
In addition, the Lonicera japonica extract may be a Lonicera japonica water extract extracted using water. The Lonicera japonica extract may be effective in alleviating symptoms of irritable bowel syndrome with diarrhea (disease code: K58.0) among irritable bowel syndrome.
According to the Gastrointestinal Symptom Rating Scale (GSRS), the diarrhea symptoms may include increased passage of stools, loose stools, and urgent need for defecation.
Therefore, the Lonicera japonica extract may alleviate symptoms of irritable bowel syndrome with diarrhea by improving the symptoms of increased passage of stools, loose stools and urgent need for defecation, which are symptoms of diarrhea.
In addition, the Lonicera japonica extract may improve borborygmus, which is a symptom related to irritable bowel disease. Borborygmus is a sound that occurs in the stomach and intestines, and when the amount of food is small and there is a lot of gas, or when the food is not digested and excessive gas is formed, a rumbling sound is generated in the process of liquid and gas advance due to the contraction of the stomach muscle, and the Lonicera japonica extract may alleviate the irritable bowel syndrome by improving the borborygmus.
The content of the Lonicera japonica extract is not particularly limited, but may be 0.1 to 10% by weight based on the total weight of the food composition, in consideration of the effect of alleviating symptoms related to irritable bowel syndrome as described above.
In the present invention, the formulations of the food composition are not particularly limited, but may be formulations such as a tablet, a capsule, a powder, a liquid, a granule, a pill, a piece, a paste, a syrup, a gel, a jelly, a caramel, a gummi, and a bar.
The food composition of the present invention may further include commonly used excipients and/or additives. The additive may be a binder, a disintegrant, a sweetener, a perfuming agent, a colorant, or a preservative, but is not limited thereto, as long as it is an additive commonly accepted in a food composition.
The excipient is not particularly limited in the present invention as long as the excipient is a substance added to facilitate ingestion of the preparation or to make a certain form. Examples of such excipients may include, but are not limited to, maltodextrin, starch, calcium carbonate, sucrose, lactose, glucose, mannitol, isomalt, xylitol, gelatin, (micro)crystalline cellulose, sorbitol, maltitol, hypromellose (HPMC), sodium lauryl sulfate, sodium alginate, calcium phosphate, and the like.
The binder is a substance that allows the shape of the preparation to be maintained, and may be, for example, one or more selected from water, an aqueous suspension of methacrylic acid copolymer, an aqueous ethyl cellulose suspension, an aqueous polyvinyl acetate suspension, and magnesium stearate.
The disintegrant is for promoting disintegration in the digestive tract in the body, and may be, for example, one or more selected from hydroxypropyl methylcellulose, bentonite, hydroxypropyl starch, sodium carboxymethylcellulose, sodium alginate, sodium lauryl sulfate, silicic anhydride, 1-hydroxypropylcellulose, dextran, starch, polyvinyl acetate, formaldehyde-treated casein and gelatin, alginic acid, amylose, guar gum, sodium bicarbonate polyvinylpyrrolidone, gum arabic, amylopectin, pectin, sodium polyphosphate, ethyl cellulose, sucrose, and magnesium aluminum silicate.
The sweetener serves to mask the bitter taste, and for example, white sugar, glucose, D-sorbitol, aspartame, licorice extract, erythritol, steviol glycosides, enzyme-treated stevioside and the like may be used.
As the perfuming agent, for example, orange oil, fruit juice extract, cinnamon oil, spearmint oil, peppermint water, vanilla, peppermint oil, rose oil, lemon oil, berry flavor, strawberry flavor, grape flavor, berry flavor, yogurt flavor and the like may be used.
As the colorant, for example, an artificial colorant such as a food synthetic colorant, a natural colorant such as a gardenia yellow colorant, a cochineal extract colorant, a caramel colorant, a Monascus colorant, and a saffron colorant and the like may be used.
As the preservative, for example, dehydroacetic acids, sorbic acids, benzoic acids, propionic acids, paraoxybenzoic acid esters and the like may be used.
In addition, the food composition of the present invention may further include additives such as commonly used a stabilizer, a thickener, an extender, or a pH adjuster.
In addition, there is no particular limitation on the type of the food. Examples of foods to which the Lonicera japonica extract may be added include meat, sausage, bread, chocolate, candy, snacks, pizza, ramen, other noodles, dairy products including ice cream, various soups, beverages, drinks, alcoholic beverages, vitamins mixtures and the like, and may include all health foods in a conventional sense.
The Lonicera japonica extract according to the present invention may be prepared by grinding Lonicera japonica, then adding an extraction solvent and heating them.
The extraction may be carried out at a temperature of 50 to 100° C., preferably 60 to 95° C. for 1 to 10 hours, preferably 3 to 7 hours.
After obtaining the Lonicera japonica extract, concentration of the extract may be further carried out, and the concentration may be performed at a temperature of 30 to 70° C. for 1 to 10 hours.
In addition, an excipient may be added for powdering of the concentrated Lonicera japonica extract, and examples of the excipient include maltodextrin, starch, lactose, crystalline cellulose and the like, and the powder may be prepared by mixing the Lonicera japonica extract and the excipient in a ratio of 10:90 to 50:50. After mixing with the excipient, the drying process of the extract may be carried out by vacuum drying, spray drying, or freeze drying, and through this process, a Lonicera japonica extract is prepared. The drying process may be performed at a temperature of 20 to 100° C. for 15 to 18 hours.
In this case, the extraction solvent may be selected from water, alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, and considering the efficacy of alleviating the symptoms of irritable bowel syndrome, the extraction solvent may be water.
In addition, by suspending the concentrated Lonicera japonica extract in water and then fractionating it with an organic solvent, an organic solvent fraction of the Lonicera japonica extract may be obtained.
Pharmaceutical Composition
The present invention relates to a pharmaceutical composition for alleviating irritable bowel syndrome, comprising a Lonicera japonica extract as an active ingredient, and the pharmaceutical composition may be a pharmaceutical composition for preventing or treating irritable bowel syndrome.
The irritable bowel syndrome is borborygmus and/or irritable bowel syndrome with diarrhea, and the irritable bowel syndrome with diarrhea may include one or more symptoms selected from the group consisting of increased passage of stools, loose stools, and urgent need for defecation.
In the present invention, the pharmaceutical composition may further include an excipient and/or an additive, and the excipient and/or additive are not particularly limited as long as they are pharmaceutically acceptable.
For example, the excipient may be maltodextrin, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil.
The additives may be a carrier, a diluent, a filler, an extender, a binder, a wetting agent, a disintegrant or a surfactant, commonly used in formulating a pharmaceutical composition.
In the present invention, the pharmaceutical composition comprising the Lonicera japonica extract as an active ingredient may be formulated into an oral or parenteral dosage form.
The formulation for oral administration may be, for example, one or more selected from the group consisting of a tablet, a pill, a hard/soft capsule, a solution, a suspension, an emulsion, a syrup and a granule.
In addition, the formulation for parenteral administration may be one or more selected from the group consisting of a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried preparation, and a suppository. The formulation for parenteral administration may be prepared in the form of a solution by mixing the Lonicera japonica extract in water with a stabilizer or buffer, and may be commercialized in a unit dosage form of ampoules or vials and injected by a subcutaneous injection, an intravenous injection, an intramuscular injection, or an intrathoracic injection.
The dosage of the pharmaceutical composition for alleviating irritable bowel syndrome comprising the Lonicera japonica extract of the present invention as an active ingredient is according to the doctor's prescription depending on factors such as the weight and age of the patient, and the specific nature and severity of the disease. However, as the oral or parenteral dosage required for adult treatment, it may be orally administered within the range of 100 to 2000 mg, preferably 100 to 1000 mg, more preferably 100 to 400 mg once or twice a day.
Hereinafter, preferred examples will be provided to help understanding the present invention, but the following examples are only for illustrating the present invention. It will be apparent to those skilled in the art that various changes and modifications may be made within the scope and technical spirit of the present invention, and it is obvious that such changes and modifications fall within the scope of the appended claims.
100 g of flower buds of Lonicera japonica were dried and ground, and then extracted by putting them in 100 mL of purified water and heating them at 60° C. for 4 hours, and filtered, and concentrated at 30° C. for 2 hours to prepare an Lonicera japonica extract.
Thereafter, it was mixed with maltodextrin as an excipient for powdering the extract, and then dried under reduced pressure at a temperature of 40° C. for 14 hours to prepare 60 g of a Lonicera japonica extract in powder form.
An experiment was conducted on the preventive or therapeutic efficacy for the symptoms of irritable bowel syndrome using the Lonicera japonica extract prepared in Example 1.
Specific test subjects, test methods, test items and test results are as follows:
1-1. Test Subject
1-2. Experimental Method
The Lonicera japonica extract prepared in Example 1 above was used as a tablet and used. The Lonicera japonica extract was prepared as a tablet of the Lonicera japonica extract (300 mg/l tablet) by a conventional method. In this case, the content of the Lonicera japonica extract contained in the tablets was set to 125 mg/l tablet.
For the test group, the Lonicera japonica extract tablets were administered twice a day, 1 tablet at a time for 8 weeks, and the administration dose of the Lonicera japonica extract was 250 mg/day.
For the control group, the tablets (300 mg/l tablet) containing only the above maltodextrin were administered twice a day, 1 tablet at a time for 8 weeks.
1-3. Experiment Result
(1) Evaluation Items
For the test group and control group that completed the experiment, a questionnaire related to the Gastrointestinal Symptom Rating Scale (GSRS) was conducted.
Table 1 below classifies the gastrointestinal and functional symptom scales.
Among the Gastrointestinal Symptom Rating Scale in Table 1 above, a questionnaire was conducted on 7 items including 3 items related to constipation symptoms, 3 items related to diarrhea syndrome and item related to irritable bowel disease-related borborygmus. The 7 items include borborygmus, which is a sound from the stomach; decreased passage of stools, hard stools, and feeling of incomplete evacuation, which are constipation symptoms; and increased passage of stools, loose stools, and urgent need for defecation symptoms, which are diarrhea symptoms.
(1) Evaluation Method
The degree of improvement for the symptoms specified in 7 items including the constipation symptoms, diarrhea symptoms and borborygmus symptom were classified into 4 grades, and then the degree of improvement for each item was evaluated for the test group and the control group, and the results were analyzed by the statistical analysis method, the Mann-Whitney U test, and the statistical analysis method before and after ingestion of the Lonicera japonica extract was tested using the Wilcoxon signed-rank test.
(2) Evaluation Results
In this case, detailed symptoms related to the above constipation and diarrhea include decreased passage of stools, hard stools, and feeling of incomplete evacuation, which are constipation symptoms; and increased passage of stools, loose stools, and urgent need for defecation symptoms, which are diarrhea symptoms.
Referring to
Referring to
In addition, Table 2 below shows the evaluation results for detailed symptoms related to constipation and diarrhea symptoms at the time points of before administration (baseline, Day 0), 4 weeks (Day 28) after administration and 8 weeks (Day 56) after administration of the Lonicera japonica extract tablets, for the test group and the control group.
Lonicera japonica
Lonicera japonica
Lonicera japonica
Lonicera japonica
Lonicera japonica
Lonicera japonica
Lonicera japonica
Referring to Table 2 above, it can be seen that the test group administered with the Lonicera japonica extract of Example significantly improved loose stools and urgent need for defecation symptoms, which are diarrhea symptoms, and borborygmus symptom, compared to the control group.
In addition, referring to Table 2 above, it can be seen that the test group administered with the Lonicera japonica extract of Example 1 significantly improved 5 out of 7 items, i.e., decreased passage of stools, feeling of incomplete evacuation, loose stools, urgent need for defecation symptoms and borborygmus, compared to before ingestion of the Lonicera japonica extracts. No significant improvement was found in the control group.
From these results, it can be seen that the Lonicera japonica extract according to the present invention is effective in improving irritable bowel syndrome, and in particular, diarrhea symptoms including loose stools, urgent need for defecation, and/or borborygmus symptoms are significantly improved, and thus, the Lonicera japonica extract is effective in improving irritable bowel syndrome with diarrhea.
8-OHdG is a biomarker for measuring oxidative stress and is used as a biomarker indicative of DNA damage in cells, and thus, it has been reported to be associated with inflammatory diseases, especially irritable bowel syndrome, which is a disease related to the gastrointestinal tract (Kauppi J, Rasanen J, Sihvo E, Nieminen U, et al., Transl Oncol. 2016; 9:336-339; Albayrak F, Uyanik M H, Dursun H, Albayrak Y, et al., South Med J. 2010; 103:753-757). That is, as the blood concentration of 8-OHdG decreases, the symptoms of irritable bowel syndrome are improved.
Accordingly, for the test group of Experimental Example 1 and control group, hematological analysis was performed on the blood concentration of 8-OHdG, and the statistical analysis method, the Mann-Whitney U test was used.
Referring to
Accordingly, it can be seen that the Lonicera japonica extract is effective in alleviating irritable bowel syndrome.
Although the present invention has been described above with reference to limited examples and drawings, the present invention is not limited thereto, and it will be apparent that various modifications and variations may be made within the scope of the technical spirit of the present invention and equivalents of the claims to be described below by those skilled in the art to which the present invention pertains.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2019-0103194 | Aug 2019 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2020/011154 | 8/21/2020 | WO |
