Claims
- 1. A conjugate composition comprising one or more, in any combination, of Structures 1-6:
- 2. The composition of claim 1 wherein linker groups R1-R6 are straight or branched chains, rings, or combinations thereof.
- 3. The composition of claim 1 wherein R1-R6 contain 2-100 atoms
- 4. The composition of claim 1 wherein R1-R6 contain 2-10 atoms.
- 5. The composition of claim 1 wherein B is a polymeric microsphere.
- 6. The composition of claim 5 wherein said polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 7. The composition of claim 6 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 8. A composition of claim 1 comprising Structure 1.
- 9. The composition of claim 8 wherein R1 contains 2-100 atoms.
- 10. The composition of claim 8 wherein R1 contains 2-10 atoms.
- 11. A composition of claim 8 in which Structure 1 is connected through its linker group R1 to an entity B, wherein B is
(a) a 2-D film or substrate; (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof; (c) an enzyme, antibody, protein, DNA, RNA, nucleotide, PNA, carbohydrate, fatty acid, lectin, peptide, receptor, dendrimer, cell, bacteria, virus, whole prokaryotic or eukaryotic organism, synthetic or natural membrane, biotin, hapten, organic monomer or polymer, or any combination thereof; (d) a chromophore, fluorophore, bio- or chemi-luminescent compound, J or H aggregate, or any combination thereof; (e) one or more of Structures 1-6 connected through their linker groups R1-R6; or any combination thereof.
- 12. The composition of claim 10 wherein entity B is a polymeric microsphere.
- 13. The composition of claim 11 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 14. The composition of claim 12 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 15. A conjugate composition of claim 1 in which Structure 2 is connected through its linker group R2 to a solid support B, wherein the solid support B is
(a) a 2-D film or substrate; or (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof.
- 16. The composition of claim 14 wherein solid support B is a polymeric microsphere.
- 17. The composition of claim 15 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 18. The composition of claim 16 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 19. A conjugate composition of claim 1 in which Structure 3 is connected through its linker group R3 to a solid support B, wherein the solid support B is
(a) a 2-D film or substrate; or (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof.
- 20. The composition of claim 19 wherein solid support B is a polymeric microsphere.
- 21. The composition of claim 20 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 22. The composition of claim 21 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 23. A conjugate composition of claim 1 in which Structure 4 is connected through its linker group R4 to a solid support B, wherein X2 is chlorine or fluorine, wherein the solid support B is
(a) a 2-D film or substrate; or (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof.
- 24. The composition of claim 23 wherein solid support B is a polymeric microsphere.
- 25. The composition of claim 24 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 26. The composition of claim 25 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 27. A conjugate composition of claim 1 in which Structure 5 is connected through its linker group R5 to a solid support B, wherein X3 is chlorine or fluorine, Y2 is nitrogen or carbon, and wherein the solid support B is
(a) a 2-D film or substrate; or (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof.
- 28. The composition of claim 27 wherein solid support B is a polymeric microsphere.
- 29. The composition of claim 28 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 30. The composition of claim 29 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 31. A conjugate composition of claim 1 in which Structure 6 is connected through its linker group R6 to a solid support B, wherein aromatic group, arom, can be substituted or unsubstituted phenyl, naphthyl or other polycyclic aromatic rings, and wherein the solid support B is
(a) a 2-D film or substrate; or (b) a micro- or nano-particle of any size or shape composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube, or any combination thereof.
- 32. The composition of claim 31 wherein solid support B is a polymeric microsphere.
- 33. The composition of claim 32 wherein the polymeric microsphere comprises polystyrene/divinyl benzene and carboxyl functional groups at least on its surface.
- 34. The composition of claim 33 wherein said microsphere further comprises one or more fluorescent dyes in differing, distinguishable molar amounts.
- 35. A method for coupling two or more entities together, comprising:
(a) providing one or more conjugate compositions of claims 1 having one or more, in any combination, of Structures 1-6 connected through their respective linker groups, R1-R6, to at least one entity B; (b) providing one or more nucleophile-containing entities comprised of:
a 2-D film or substrate; a micro- or nano-particle of any size or shape, composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube or any combination thereof; an enzyme, antibody, protein, DNA, RNA, nucleotide, PNA, carbohydrate, fatty acid, lectin, peptide, receptor, chromophore, fluorophore, bio- or chemi-luminescent compound, J or H aggregate, cell, bacteria, virus, whole prokaryotic or eukaryotic organism, synthetic or natural membrane, biotin, hapten, organic monomer or polymer, or dendrimer, or any combination thereof; and (c) reacting said one or more conjugate compositions with said one or more nucleophile-containing entities to produce at least one entity B linked through one or more linker groups of Structures 1-6 to said one or more nucleophile-containing entities.
- 36. A method for the synthesis of compositions of claim 1 wherein:
(a) the one or more, in any combination, of Structures 1-6 of claim 1 are conjugated to said entity B before the fabrication of said entity B; (b) the one or more, in any combination, of Structures 1-6 of claim 1 are conjugated to said entity B during the fabrication of said entity B; or (c) the one or more, in any combination, of Structures 1-6 of claim 1 are conjugated to said entity B after the fabrication of said entity B.
- 37. A method of crosslinking one or more nucleophilile-containing entities to at least one other entity comprising reacting a composition of Structure 1 of claim 1, wherein:
(a) n is 0, 1, 2, or 3 (b) X and Y are oxygen and/or sulfur in any combination; (c) Z and R1 are chloride; fluoride; 2,3,5,6-tetrafluoro-4-sulfo-phenoxide; N-hydroxysuccinimide; and similar electrophilic groups or any combination thereof; with one or more nucleophile-containing entities, comprised of: a 2-D film or substrate; a micro- or nano-particle of any size or shape, composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube or any combination thereof; an enzyme, antibody, protein, DNA, RNA, nucleotide, PNA, carbohydrate, fatty acid, lectin, peptide, receptor, chromophore, fluorophore, bio- or chemi-luminescent compound, J or H aggregate, cell, bacteria, virus, whole prokaryotic or eukaryotic organism, synthetic or natural membrane, biotin, hapten, organic monomer or polymer, or dendrimer, or any combination thereof; to provide one or more nucleophile-containing entities crosslinked to at least one other entity.
- 38. A bridge group composition comprising two or more of Structures 1-6 of claim 1, in any combination, joined together through their respective linker groups R1-R6, wherein said joining is one or more of end-to-end, branched, or dendritic, in any combination.
- 39. A method for crosslinking two or more nucleophile-containing entities
(a) providing one or more bridge group compositions of claim 38;(b) providing one or more nucleophile-containing entities comprised of:
a 2-D film or substrate; a nucro- or nano-particle of any size or shape, composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube or any combination thereof; an enzyme, antibody, protein, DNA, RNA, nucleotide, PNA, carbohydrate, fatty acid, lectin, peptide, receptor, chromophore, fluorophore, bio- or chemi-luminescent compound, J or H aggregate, cell, bacteria, virus, whole prokaryotic or eukaryotic organism, synthetic or natural membrane, biotin, hapten, organic monomer or polymer, or dendrimer, or any combination thereof; and (c) reacting said one or more bridge group compositions with said one or more nucleophile-containing entities to produce at least two crosslinked nucleophile-containing entities.
- 40. A bridge group composition comprising one or more of Structures 1-6 of claim 1, in any combination, conjugated by their respective linker groups R1-R6 to one or more nucleophilic groups, wherein if two or more of Structure 1-6 are joined together, they are joined together through their respective linker groups R1-R6, wherein said joining is one or more of end-to-end, branched, or dendritic, in any combination.
- 41. A method for crosslinking one or more nucleophile-containing entities with one or more entities conjugated with one of more of Structures 1-6 of claim 1, comprising:
(a) providing one or more bridge group compositions of claim 40;(b) providing one or more of the compositions of claim 1;(c) providing one or more nucleophile-containing entities comprised of:
a 2-D film or substrate; a micro- or nano-particle of any size or shape, composed of organic polymer, MIPS, glass, metal, clay, resin, diatomaceous earth, zeolite, inorganic crystal, semiconductor particle, semiconductor nanocrystal, magnetic particle, fullerene, nanotube or any combination thereof; an enzyme, antibody, protein, DNA, RNA, nucleotide, PNA, carbohydrate, fatty acid, lectin, peptide, receptor, chromophore, fluorophore, bio- or chemi-luminescent compound, J or H aggregate, cell, bacteria, virus, whole prokaryotic or eukaryotic organism, synthetic or natural membrane, biotin, hapten, organic monomer or polymer, or dendrimer, or any combination thereof; and (d) reacting said one or more bridge group compositions with said one or more nucleophile-containing entities and one or more compositions of claim 1 to produce at least two crosslinked entities.
1. CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to U.S. provisional Application No. 60/331,312, filed Nov. 14, 2001, the disclosure of which is hereby incorporated herein by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60331312 |
Nov 2001 |
US |