Patent Application
20090264411
The present invention relates to a class of fused thiophene derivatives and to their use in therapy. More particularly, the invention is concerned with 4,5,6,7-tetrahydro-thieno[2,3-c]azepin-8-one derivatives, and analogues thereof, which are substituted in the 2-position by a substituted anilino moiety. These compounds are selective inhibitors of MEK (MAPKK) enzymes, and are accordingly of benefit as pharmaceutical agents, especially in the treatment of adverse inflammatory, autoimmune, cardiovascular, proliferative (including oncological) and nociceptive conditions.
MEK enzymes are implicated in a variety of physiological and pathological functions that are believed to be operative in a range of human diseases. These functions are summarised in paragraphs [0004] and [0005] of US 2005/0049276 A1.
The compounds of use in the present invention, being potent and selective MEK inhibitors, are therefore beneficial in the treatment and/or prevention of various human ailments. These include autoimmune and inflammatory disorders such as rheumatoid arthritis, osteoarthritis, multiple sclerosis, asthma, inflammatory bowel disease, psoriasis and transplant rejection; cardiovascular disorders including thrombosis, cardiac hypertrophy, hypertension, and irregular contractility of the heart (e.g. during heart failure); proliferative disorders such as restenosis, and oncological conditions including leukaemia, glioblastoma, lymphoma, melanoma, and human cancers of the liver, bone, skin, brain, pancreas, lung, breast, stomach, colon, rectum, prostate, ovary and cervix; and pain and nociceptive disorders, including chronic pain and neuropathic pain.
In addition, the compounds of use in the present invention may be beneficial as pharmacological standards for use in the development of new biological tests and in the search for new pharmacological agents. Thus, the compounds of use in this invention may be useful as radioligands in assays for detecting compounds capable of binding to human MEK enzymes.
WO 2005/023818 describes a broad-ranging class of compounds based on a fused heterobicyclic ring system, which generically encompasses 5,6-dihydro-1-benzothiophen-7(4H)-one and 5,6-dihydrothieno[2,3-c]pyridin-7(4H)-one derivatives attached to a substituted anilino moiety but nowhere specifically discloses any actual compound of this type. Whilst no discrete pharmacological activity is ascribed to the compounds described therein, they are nevertheless stated to be useful inter alia in the treatment of cell proliferative diseases such as cancer.
Nowhere in the prior art, however, is there the precise disclosure of a class of 4,5,6,7-tetrahydrothieno[2,3-c]azepin-8-one derivatives, and analogues thereof, attached at the 2-position to a substituted anilino moiety. It has now been found that such compounds are particularly valuable as selective inhibitors of MEK enzymes.
The compounds of the present invention are potent and selective MEK inhibitors having a binding affinity (IC50) for the human MEK1 and/or MEK2 enzyme of 50 μM or less, generally of 20 μM or less, usually of 5 μM or less, typically of 1 μM or less, suitably of 500 nM or less, ideally of 100 nM or less, and preferably of 20 nM or less (the skilled person will appreciate that a lower IC50 figure denotes a more active compound). The compounds of the invention may possess at least a 10-fold selective affinity, typically at least a 20-fold selective affinity, suitably at least a 50-fold selective affinity, and ideally at least a 100-fold selective affinity, for the human MEK1 and/or MEK2 enzyme relative to other human kinases.
The present invention provides a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof:
wherein
-X- represents a group of formula (a), (b), (c), (d), (e), (f) or (g):
Y represents oxygen, sulphur or N—R8;
R1 and R2 independently represent hydrogen; or C1-6 alkyl, C3-7 cycloalkyl, C3-7 cycloalkyl(C1-6)alkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl-(C1-6)alkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents; or
R1 and R2, when both are attached to the same carbon atom, represent, when taken together with the carbon atom to which they are both attached, C3-7 cycloalkyl or C3-7 heterocycloalkyl, either of which groups may be optionally substituted by one or more substituents; or
R1 and R2, when attached to adjacent carbon atoms, represent, when taken together with the carbon atoms to which they are attached, C5-7 cycloalkyl, phenyl or heteroaryl, any of which groups may be optionally benzo-fused and/or substituted by one or more substituents;
R3 represents hydrogen, C1-6 alkyl, C3-7 heterocycloalkenyl (optionally substituted by one or two methyl groups), cyano, —CO2Ra, —CORb, —CONRbRc, —SO2NRbRc, —CON(ORb)Rc, —CON(Rc)CORb, —CON(Rc)SO2Rb, —SO2N(Rc)CORb, —CON(Rd)NRbRc, —C(═NRc)NRbRc or —CON(Rd)C(═NRc)NRbRc; or
R3 represents an optionally substituted five-membered heteroaromatic ring selected from furan, thiophene, pyrrole, oxazole, thiazole, isoxazole, isothiazole, imidazole, pyrazole, oxadiazole, thiadiazole, triazole and tetrazole; or
R3 represents an optionally substituted six-membered heteroaromatic ring selected from pyridine, pyrazine, pyrimidine, pyridazine and triazine;
R4a and R4b independently represent hydrogen, halogen, cyano, nitro, C1-6 alkyl, trifluoromethyl, C1-6 alkoxy, trifluoromethoxy, C1-6 alkylthio, C1-6 alkylsulphinyl or C1-6 alkylsulphonyl;
R5 represents halogen, nitro, cyano, C1-6 alkyl, C2-6 alkynyl, hydroxy(C1-6)alkyl or formyl;
R6 represents hydrogen, C1-6 alkyl, formyl, C2-6 alkylcarbonyl, trifluoromethylcarbonyl or C1-6 alkylsulphonyl;
R7 and R8 independently represent hydrogen or C1-6 alkyl;
Ra represents hydrogen, C1-6 alkyl or C3-7 heterocycloalkyl(C1-6)alkyl;
Rb represents hydrogen; or C1-6 alkyl, C3-7 cycloalkyl, C3-7 cycloalkyl(C1-6)alkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl, C4-9 heterobicycloalkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents; and
Rc represents hydrogen or C1-6 alkyl (optionally substituted by hydroxy); or
Rb and Rc, when taken together with the nitrogen atom to which they are both attached, represent azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, homopiperidinyl, homomorpholinyl or homopiperazinyl, any of which groups may be optionally substituted by one or more substituents; and
Rd and Re independently represent hydrogen or C1-6 alkyl.
The present invention also provides a compound of formula (I) as depicted above, or a pharmaceutically acceptable salt or solvate thereof, wherein
R3 represents hydrogen, cyano, —CO2Ra, —CORb, —CONRbRc, —SO2NRbRc, —CON(ORb)Rc, —CON(Rc)CORb, —CON(Rc)SO2Rb, —SO2N(Rc)CORb, —CON(Rd)NRbRc, —C(═NRe)NRbRc or —CON(Rd)C(═NRc)NRbRc; or
R3 represents an optionally substituted five-membered heteroaromatic ring selected from furan, thiophene, pyrrole, oxazole, thiazole, isoxazole, isothiazole, imidazole, pyrazole, oxadiazole, thiadiazole, triazole and tetrazole; or
R3 represents an optionally substituted six-membered heteroaromatic ring selected from pyridine, pyrazine, pyrimidine, pyridazine and triazine;
R5 represents halogen, nitro, C1-6 alkyl, hydroxy(C1-6)alkyl or formyl;
Ra represents hydrogen or C1-6 alkyl;
Rb represents hydrogen; or C1-6 alkyl, C3-7 cycloalkyl, C3-7 cycloalkyl(C1-6)alkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents; and
Rc represents hydrogen or C1-6 alkyl; or
Rb and Rc, when taken together with the nitrogen atom to which they are both attached, represent azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, homopiperidinyl or homomorpholinyl, any of which groups may be optionally substituted by one or more substituents; and
R1, R2, R4a, R4b, Rd and Rc are as defined above.
Where a group in the compounds of formula (I) above is stated to be optionally substituted, this group may be unsubstituted, or substituted by one or more substituents. Typically, such a group will be unsubstituted, or substituted by one or two substitutents. Suitably, such a group will be unsubstituted or monosubstituted.
For use in medicine, the salts of the compounds of formula (I) will be pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds of the invention or of their pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds of this invention include acid addition salts which may, for example, be formed by mixing a solution of the compound of the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid or phosphoric acid. Furthermore, where the compounds of the invention carry an acidic moiety, e.g. carboxy, suitable pharmaceutically acceptable salts thereof may include alkali metal salts, e.g. sodium or potassium salts; alkaline earth metal salts, e.g. calcium or magnesium salts; and salts formed with suitable organic ligands, e.g. quaternary ammonium salts.
The present invention includes within its scope solvates of the compounds of formula (I) above. Such solvates may be formed with common organic solvents, e.g. hydrocarbon solvents such as benzene or toluene; chlorinated solvents such as chloroform or dichloromethane; alcoholic solvents such as methanol, ethanol or isopropanol; ethereal solvents such as diethyl ether or tetrahydrofuran; or ester solvents such as ethyl acetate. Alternatively, the solvates of the compounds of formula (I) may be formed with water, in which case they will be hydrates.
Suitable alkyl groups which may be present on the compounds of the invention include straight-chained and branched C1-6 alkyl groups, for example C1-4 alkyl groups. Typical examples include methyl and ethyl groups, and straight-chained or branched propyl, butyl and pentyl groups. Particular alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl and 2,2-dimethylpropyl. Derived expressions such as “C1-6 alkoxy”, “C1-6 alkylthio”, “C1-6 alkylsulphonyl” and “C1-6 alkylamino” are to be construed accordingly.
Specific C3-7 cycloalkyl groups are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
Suitable aryl groups include phenyl and naphthyl, preferably phenyl.
Suitable aryl(C1-6)alkyl groups include benzyl, phenylethyl, phenylpropyl and naphthylmethyl.
Suitable heterocycloalkyl groups, which may comprise benzo-fused analogues thereof, include azetidinyl, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, indolinyl, thiazolidinyl, imidazolidinyl, tetrahydropyranyl, piperidinyl, 1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, piperazinyl, 1,2,3,4-tetrahydroquinoxalinyl, morpholinyl, thiomorpholinyl, homopiperidinyl and homopiperazinyl.
A typical C3-7 heterocycloalkenyl group is dihydroimidazolyl (e.g. 4,5-dihydro-1H-imidazol-2-yl).
A typical C4-9 heterobicycloalkyl group is azabicyclo[2.2.2]octyl (e.g. quinuclidin-3-yl).
Suitable heteroaryl groups include furyl, benzofuryl, dibenzofuryl, thienyl, benzothienyl, pyrrolyl, indolyl, pyrrolo[2,3-b]pyridinyl, pyrazolyl, indazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, imidazo[1,2-a]pyridinyl, benzimidazolyl, oxadiazolyl, thiadiazolyl, triazolyl, benzotriazolyl, tetrazolyl, pyridinyl, quinolinyl, isoquinolinyl, pyridazinyl, pyrimidinyl and pyrazinyl groups.
The term “halogen” as used herein is intended to include fluorine, chlorine, bromine and iodine atoms.
Where the compounds of formula (I) have one or more asymmetric centres, they may accordingly exist as enantiomers. Where the compounds of the invention possess two or more asymmetric centres, they may additionally exist as diastereomers. The invention is to be understood to extend to all such enantiomers and diastereomers, and to mixtures thereof in any proportion, including racemates. Formula (I) and the formulae depicted hereinafter are intended to represent all individual stereoisomers and all possible mixtures thereof, unless stated or shown otherwise. In addition, compounds of formula (I) may exist as tautomers, for example keto (CH2C═O)-enol (CH═CHOH) tautomers. Formula (I) and the formulae depicted hereinafter are intended to represent all individual tautomers and all possible mixtures thereof, unless stated or shown otherwise.
Specific sub-classes of compounds in accordance with the present invention are represented by the compounds of formula (IA), (IB), (IC), (ID), (IE), (IF) and (IG):
wherein Y, R1, R2, R3, R4a, R4b, R5, R6 and R7 are as defined above.
Selected sub-classes of compounds in accordance with the present invention are represented by the compounds of formula (IA), (IB) and (IC) as depicted above. In one embodiment, the compounds according to the present invention are represented by formula (IA) as depicted above. In another embodiment, the compounds according to the present invention are represented by formula (IB) as depicted above. In a further embodiment, the compounds according to the present invention are represented by formula (IC) as depicted above.
A particular sub-class of compounds in accordance with the present invention is represented by the compounds of formula (IC) as depicted above.
In the compounds of formula (I), -X- typically represents a group of formula (a), (b) or (c) as depicted above. In one embodiment, -X- represents a group of formula (a) as depicted above. In another embodiment, -X- represents a group of formula (b) as depicted above. In a further embodiment, -X- represents a group of formula (c) as depicted above.
In the compounds of formula (I), -X- suitably represents a group of formula (c) as depicted above.
In one embodiment, Y is oxygen. In another embodiment, Y is sulphur. In a further embodiment, Y is N—R8 in which R8 is as defined above.
Suitably, R1 represents hydrogen; or C1-6 alkyl, aryl or heteroaryl, any of which groups may be optionally substituted by one or more substituents.
Suitably, R2 represents hydrogen or optionally substituted C1-6 alkyl.
Examples of typical substituents on R1 and/or R2 include halogen, cyano, nitro, C1-6 alkyl, trifluoromethyl, hydroxy, C1-6 alkoxy, difluoromethoxy, trifluoromethoxy, aryloxy, C1-6 alkylthio, C1-6 alkylsulphonyl, amino, C1-6 alkylamino, di(C1-6)alkylamino, C2-6 alkylcarbonylamino, C2-6 alkoxycarbonylamino, C1-6 alkylsulphonylamino, formyl, C2-6 alkylcarbonyl, carboxy, C2-6 alkoxycarbonyl, aminocarbonyl, C1-6 alkylamino-carbonyl, di(C1-6)alkylaminocarbonyl, aminosulphonyl, C1-6 alkylaminosulphonyl and di(C1-6)alkylaminosulphonyl; especially halogen, C1-6 alkoxy or C1-6 alkylthio.
Examples of particular substituents on R1 and/or R2 include fluoro, chloro, bromo, cyano, nitro, methyl, trifluoromethyl, hydroxy, methoxy, difluoromethoxy, trifluoro-methoxy, phenoxy, methylthio, methylsulphonyl, amino, methylamino, dimethylamino, acetylamino, methoxycarbonylamino, methylsulphonylamino, formyl, acetyl, carboxy, methoxycarbonyl, aminocarbonyl, methylaminocarbonyl, dimethylaminocarbonyl, aminosulphonyl, methylaminosulphonyl and dimethylaminosulphonyl; especially chloro, methoxy or methylthio.
Typical values of R1 include hydrogen, methyl, n-propyl, isopropyl, phenyl, chlorophenyl, methoxyphenyl, methylthiophenyl and furyl. In one embodiment, R1 is hydrogen. A particular value of R1 is methyl.
Typical values of R2 include hydrogen and methyl. In one embodiment, R2 is hydrogen. In another embodiment, R2 is C1-6 alkyl, especially methyl.
Alternatively, R1 and R2, when both are attached to the same carbon atom, may together form an optionally substituted spiro linkage. Thus, R1 and R2, when both are attached to the same carbon atom, may represent, when taken together with the carbon atom to which they are both attached, C3-7 cycloalkyl or C3-7 heterocycloalkyl, either of which groups may be unsubstituted, or substituted by one or more, typically by one or two, substituents. In this context, R1 and R2, when taken together with the carbon atom to which they are both attached, may suitably represent an optionally substituted cyclopentyl, cyclohexyl, pyrrolidine or piperidine ring, especially cyclopentyl or cyclohexyl.
Alternatively, R1 and R2, when attached to adjacent carbon atoms, may together form an optionally benzo-fused and/or substituted cycloalkyl, phenyl or heteroaryl (e.g. pyridinyl) ring fused to the ring containing the variable X. Thus, R1 and R2, when attached to adjacent carbon atoms, may represent, when taken together with the carbon atoms to which they are attached, C5-7 cycloalkyl, phenyl or heteroaryl (e.g. pyridinyl), any of which groups may be benzo-fused and/or unsubstituted, or substituted by one or more, typically by one or two, substituents. In this context, in one embodiment, R1 and R2, when taken together with the adjacent carbon atoms to which they are attached, suitably represent a phenyl ring fused to the ring containing the variable X. Also in this context, in another embodiment, R1 and R2, when taken together with the adjacent carbon atoms to which they are attached, suitably represent a benzo-fused cyclopentyl ring, i.e. an indanyl moiety fused to the ring containing the variable X.
Typically, Ra represents hydrogen or C1-6 alkyl. Suitably, Ra represents hydrogen, methyl or ethyl, especially hydrogen or ethyl. In one embodiment, Ra represents hydrogen. In another embodiment, Ra represents methyl. In a further embodiment, Ra represents ethyl. In a still further embodiment, Ra represents C3-7 heterocycloalkyl-(C1-6)alkyl, especially piperidinylmethyl.
In a favoured embodiment, Rb represents hydrogen; or C1-6 alkyl, C3-7 cycloalkyl-(C1-6)alkyl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl, C4-9 heterobicycloalkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents.
In an illustrative embodiment, Rb represents hydrogen; or C1-6 alkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents.
Suitably, Rb represents hydrogen; or C1-6 alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents.
In a definitive embodiment, Rb represents hydrogen; or methyl, ethyl, propyl, butyl, pentyl, hexyl, cyclohexylmethyl, benzyl, phenylethyl, azetidinyl, tetrahydrofuryl, tetrahydrothienyl, pyrrolidinyl, piperidinyl, homopiperidinyl, quinuclidinyl, azetidinylmethyl, tetrahydrofurylmethyl, pyrrolidinylmethyl, pyrrolidinylethyl, pyrrolidinylpropyl, thiazolidinylmethyl, imidazolidinylethyl, piperidinylmethyl, piperidinylethyl, tetrahydroquinolinylmethyl, piperazinylpropyl, morpholinylethyl, morpholinylpropyl, pyridinyl, indolylethyl, pyrazolylethyl, imidazolylmethyl, imidazolylethyl, benzimidazolylmethyl, triazolylethyl, pyridinylmethyl or pyridinylethyl, any of which groups may be optionally substituted by one or more substituents.
Definitive examples of suitable substituents on Rb, or on the cyclic moiety —NRbRc, include C1-6 alkyl, C1-6 alkoxy, C1-6 alkylamino(C1-6)alkoxy, C1-6 alkoxy(C1-6)-alkyl, C1-6 alkylthio, C1-6 alkylsulphonyl, hydroxy, hydroxy(C1-6)alkyl, amino(C1-6)alkyl, nitro(C1-6)alkyl, cyano, trifluoromethyl, oxo, C2-6 alkylcarbonyl, carboxy, C2-6 alkoxycarbonyl, amino, C1-6 alkylamino, di(C1-6)alkylamino, bis[hydroxy(C1-6)alkyl]-amino, C1-6 alkylamino(C1-6)alkylamino, phenylamino, pyridinylamino, C2-6 alkylcarbonylamino, C2-6 alkoxycarbonylamino, [(C2-6)alkoxycarbonyl][(C1-6)alkyl]-amino, bis[(C2-6)alkoxycarbonyl(C1-6)alkyl]amino, C2-6 alkoxycarbonylamino(C1-6)alkyl, aminocarbonyl and guanidinyl.
Examples of typical substituents on Rb, or on the cyclic moiety —NRbRc, include C1-6 alkyl, C1-6 alkoxy, hydroxy, hydroxy(C1-6)alkyl, amino(C1-6)alkyl, (amino)(hydroxy)-(C1-6)alkyl, halogen, oxo, C2-6 alkylcarbonyl, carboxy, C2-6 alkoxycarbonyl, di(C1-6)alkylhydrazinylcarbonyl, amino, C1-6 alkylamino, di(C1-6)alkylamino, C2-6 alkylcarbonylamino, aminocarbonylamino, aminocarbonyl, C1-6 alkylaminocarbonyl, di(C1-6)alkylaminocarbonyl, aminosulfonyl, C1-6 alkylsulfonyl and C1-6 alkylaminocarbonyl(C1-6)alkyl; especially C1-6 alkyl, hydroxy, hydroxy(C1-6)alkyl or amino.
Definitive examples of specific substituents on Rb, or on the cyclic moiety —NRbRc, include methyl, ethyl, isopropyl, methoxy, isopropoxy, methylaminoethoxy, methoxymethyl, methylthio, ethylthio, methylsulphonyl, hydroxy, hydroxymethyl, hydroxyethyl, aminomethyl, nitromethyl, cyano, trifluoromethyl, oxo, acetyl, carboxy, methoxycarbonyl, ethoxycarbonyl, tert-butoxycarbonyl, amino, methylamino, ethylamino, dimethylamino, bis[hydroxyethyl]amino, ethylaminoethylamino, phenylamino, pyridinylamino, acetylamino, tert-butoxycarbonylamino, (tert-butoxy-carbonyl)(methyl)amino, bis(ethoxycarbonylmethyl)amino, tert-butoxycarbonylamino-methyl, aminocarbonyl and guanidinyl.
Examples of particular substituents on Rb, or on the cyclic moiety —NRcRc, include methyl, methoxy, hydroxy, hydroxymethyl, 2-hydroxyethyl, aminomethyl, 2-amino-3-hydroxypropyl, fluoro, oxo, acetyl, carboxy, methoxycarbonyl, ethoxycarbonyl, tert-butoxycarbonyl, dimethylhydrazinylcarbonyl, amino, methylamino, 1,3-dimethylbutylamino, dimethylamino, acetylamino, aminocarbonylamino, aminocarbonyl, ethylaminocarbonyl, diethylaminocarbonyl, aminosulfonyl, methylsulfonyl and methylaminocarbonylmethyl; especially methyl, hydroxy, hydroxymethyl or amino.
Typically, Rb represents C1-6 alkyl, optionally substituted by one or more, preferably one or two, hydroxy groups.
Specific values of Rb include hydrogen, methyl, carboxymethyl, aminocarbonyl-methyl, methoxyethyl (especially 2-methoxyethyl), methylaminoethoxyethyl (especially 2-[2-(methylamino)ethoxy]ethyl), ethylthioethyl (especially 2-(ethylthio)ethyl), methylsulphonylethyl (especially 2-(methylsulphonyl)ethyl), hydroxyethyl (especially 2-hydroxyethyl), cyanoethyl (especially 2-cyanoethyl), (hydroxy)(trifluoromethyl)ethyl (especially 2-hydroxy-3,3,3-trifluoropropyl), carboxyethyl (especially 2-carboxyethyl), ethoxycarbonylethyl (especially 2-(ethoxycarbonyl)ethyl), aminoethyl (especially 2-aminoethyl), (amino)(carboxy)ethyl (especially 2-amino-2-carboxyethyl), methylamino-ethyl (especially 2-(methylamino)ethyl), dimethylaminoethyl (especially 2-(dimethylamino)ethyl), bis(hydroxyethyl)aminoethyl (especially 2-[bis(2-hydroxyethyl)amino]-ethyl), ethylaminoethylaminoethyl (especially 2-[2-(ethylamino)ethylamino]ethyl), phenylaminoethyl (especially 2-(phenylamino)ethyl), pyridinylaminoethyl (especially 2-(pyridin-2-ylamino)ethyl), acetylaminoethyl (especially 2-(acetylamino)ethyl), (tert-butoxycarbonylamino)(carboxy)ethyl (especially 2-(tert-butoxycarbonylamino)-2-carboxyethyl), (tert-butoxycarbonyl)(methyl)aminoethyl (especially 2-[N-(tert-butoxycarbonyl)-N-methylamino]ethyl), aminocarbonylethyl (especially 1-(aminocarbonyl)ethyl), propyl, methoxypropyl (especially 2-methoxy-1-methylethyl), isopropoxypropyl (especially 3-isopropoxypropyl), hydroxypropyl (especially 2-hydroxypropyl or 3-hydroxypropyl), dihydroxypropyl (especially 2,3-dihydroxypropyl), (carboxy)(methylthio)propyl (especially 1-carboxy-3-(methylthio)propyl), ethoxycarbonylpropyl (especially 2-ethoxycarbonyl-1-methylethyl), aminopropyl (especially 3-aminopropyl), (amino)(hydroxy)propyl (especially 3-amino-2-hydroxypropyl), methylaminopropyl (especially 3-(methylamino)propyl), ethylaminopropyl (especially 3-(ethylamino)propyl), dimethylaminopropyl (especially 3-(dimethylamino)propyl), tert-butyl, hydroxybutyl (especially 1,1-dimethyl-2-hydroxyethyl or 2-hydroxy-2-methylpropyl), dihydroxybutyl (especially 3,4-dihydroxybutyl), aminobutyl (especially 2-amino-2-methylpropyl or 4-aminobutyl), (carboxy)(guanidinyl)butyl (especially 1-carboxy-4-(guanidinyl)butyl), aminopentyl (especially 3-amino-2,2-dimethylpropyl), dimethylaminopentyl (especially 3-(dimethylamino)-2,2-dimethylpropyl), hydroxyhexyl (especially 1-(tert-butyl)-2-hydroxyethyl), hydroxycyclohexylmethyl, (aminomethyl)-cyclohexylmethyl, methoxybenzyl, (hydroxy)(phenyl)ethyl, (oxo)(phenyl)ethyl, (carboxy)(hydroxyphenyl)ethyl, azetidinyl, (oxo)tetrahydrofuryl, (dioxo)tetrahydrothienyl, pyrrolidinyl, methylpyrrolidinyl, tert-butoxycarbonylpyrrolidinyl, piperidinyl, methylpiperidinyl, (oxo)homopiperidinyl, quinuclidinyl, azetidinylmethyl, hydroxyazetidinylmethyl, tert-butoxycarbonylazetidinylmethyl, (tert-butoxycarbonyl)(hydroxy)-azetidinylmethyl, tetrahydrofurylmethyl, pyrrolidinylmethyl, ethylpyrrolidinylmethyl, pyrrolidinylethyl, methylpyrrolidinylethyl, (carboxy)(isopropyl)(oxo)pyrrolidinylethyl, (oxo)pyrrolidinylpropyl, thiazolidinylmethyl, (oxo)imidazolidinylethyl, piperidinylmethyl, methylpiperidinylmethyl, tert-butoxycarbonylpiperidinylmethyl, piperidinylethyl, tetrahydroquinolinylmethyl, methylpiperazinylpropyl, morpholinylethyl, morpholinylpropyl, pyridinyl, aminopyridinyl, (carboxy)indolylethyl, dimethylpyrazolylethyl, methylimidazolylmethyl, imidazolylethyl, benzimidazolylmethyl, triazolylethyl, pyridinylmethyl and pyridinylethyl.
Typical values of Rb include hydrogen, methyl, aminoethyl (especially 2-aminoethyl), hydroxypropyl (especially 2-hydroxypropyl or 3-hydroxypropyl), aminopropyl (especially 3-aminopropyl), dihydroxypropyl (especially 2,3-dihydroxypropyl), (amino)(hydroxy)propyl (especially 3-amino-2-hydroxypropyl), hydroxybutyl (especially 1,1-dimethyl-2-hydroxyethyl or 2-hydroxy-2-methylpropyl), aminobutyl (especially 2-amino-2-methylpropyl or 4-aminobutyl), dihydroxybutyl (especially 3,4-dihydroxybutyl), piperidinyl (especially piperidin-3-yl), methylpiperidinyl (especially 1-methylpiperidin-4-yl), piperidinylmethyl (especially piperidin-4-ylmethyl), imidazolylethyl [especially 2-(imidazol-5-yl)ethyl] and pyridinylethyl [especially 2-(pyridin-3-yl)ethyl or 2-(pyridin-4-yl)ethyl].
Suitably, Rc represents hydrogen or C1-6 alkyl. In one embodiment, Rc is hydrogen. In another embodiment, Rc represents C1-6 alkyl, especially methyl or ethyl, particularly methyl. In a further embodiment, Rc represents hydroxy-substituted C1-6 alkyl, e.g. hydroxyethyl (especially 2-hydroxyethyl).
Alternatively, the moiety —NRbRc may suitably represent azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, morpholin-4-yl, thiomorpholin-4-yl, piperazin-1-yl, homopiperidin-1-yl, homomorpholin-4-yl or homopiperazin-1-yl, any of which groups may be optionally substituted by one or more substituents. Favourably, the moiety —NRbRc may suitably represent azetidin-1-yl, pyrrolidin-1-yl, piperidin-1-yl, morpholin-4-yl, piperazin-1-yl or homopiperazin-1-yl, any of which groups may be optionally substituted by one or more substituents. Typically, the moiety —NRbRc may suitably represent azetidin-1-yl, pyrrolidin-1-yl or piperazin-1-yl, any of which groups may be optionally substituted by one or more substituents.
Typical substituents on the cyclic moiety —NRbRc include C1-6 alkyl, C1-6 alkoxy(C1-6)alkyl, hydroxy, hydroxy(C1-6)alkyl, amino(C1-6)alkyl, nitro(C1-6)alkyl, oxo, C2-6 alkylcarbonyl, carboxy, C2-6 alkoxycarbonyl, amino, C2-6 alkylcarbonylamino, C2-6 alkoxycarbonylamino, bis[(C2-6)alkoxycarbonyl(C1-6)alkyl]amino, C2-6 alkoxycarbonylamino(C1-6)alkyl and aminocarbonyl. Specific substituents include methyl, ethyl, methoxymethyl, hydroxy, hydroxymethyl, hydroxyethyl, aminomethyl, nitromethyl, oxo, acetyl, carboxy, methoxycarbonyl, tert-butoxycarbonyl, amino, acetylamino, tert-butoxycarbonylamino, bis(ethoxycarbonylmethyl)amino, tert-butoxycarbonylaminomethyl and aminocarbonyl.
Suitably, the cyclic moiety —NRbRc may be substituted by C1-6 alkyl, hydroxy or hydroxy(C1-6)alkyl; especially methyl, hydroxy or hydroxymethyl.
Definitive values of —NRbRc include hydroxyazetidin-1-yl, (hydroxy)(nitro-methyl)azetidin-1-yl, aminoazetidin-1-yl, (aminomethyl)azetidin-1-yl, (aminomethyl)-(hydroxy)azetidin-1-yl, (tert-butoxycarbonylaminomethyl)azetidin-1-yl, pyrrolidin-1-yl, (methoxymethyl)pyrrolidin-1-yl, hydroxypyrrolidin-1-yl, (hydroxymethyl)pyrrolidin-1-yl, (aminomethyl)pyrrolidin-1-yl, carboxypyrrolidin-1-yl, (methoxycarbonyl)pyrrolidin-1-yl, aminopyrrolidin-1-yl, (acetylamino)pyrrolidin-1-yl, (tert-butoxycarbonylamino)-pyrrolidin-1-yl, [bis(ethoxycarbonylmethyl)amino]pyrrolidin-1-yl, (tert-butoxycarbonyl-aminomethyl)pyrrolidin-1-yl, hydroxypiperidin-1-yl, (hydroxymethyl)piperidin-1-yl, (hydroxyethyl)piperidin-1-yl, carboxypiperidin-1-yl, (aminocarbonyl)piperidin-1-yl, piperazin-1-yl, methylpiperazin-1-yl, ethylpiperazin-1-yl, (hydroxyethyl)piperazin-1-yl, oxopiperazin-1-yl, acetylpiperazin-1-yl, carboxypiperazin-1-yl, (tert-butoxycarbonyl)-(carboxy)piperazin-1-yl, morpholin-4-yl, dimethylmorpholin-4-yl, (hydroxymethyl)-morpholin-4-yl and homopiperazin-1-yl.
Particular values of —NRbRc include 3-hydroxyazetidin-1-yl, pyrrolidin-1-yl, 3-hydroxypyrrolidin-1-yl, 2-(hydroxymethyl)pyrrolidin-1-yl, piperazin-1-yl and 4-methylpiperazin-1-yl.
In one embodiment, Rd is hydrogen. In another embodiment, Rd represents C1-6 alkyl, especially methyl.
In one embodiment, Re is hydrogen. In another embodiment, Re represents C1-6 alkyl, especially methyl.
Where R3 represents a five-membered heteroaromatic ring, this ring may be optionally substituted by one or, where possible, two substituents. As will be appreciated, where R3 represents an oxadiazole, thiadiazole or tetrazole ring, only one substituent will be possible; otherwise, one or two optional substituents may be accommodated around the five-membered heteroaromatic ring R3. Where R3 represents a six-membered heteroaromatic ring, this ring may be optionally substituted by one or more substituents, typically by one or two substituents. Examples of suitable substituents on the five-membered or six-membered heteroaromatic ring as specified for R3 include C1-6 alkyl, C3-7 cycloalkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, heteroaryl, heteroaryl(C1-6)-alkyl, C1-6 alkoxy, C1-6 alkylthio, amino, C1-6 alkylamino, di(C1-6)alkylamino, halogen, cyano and trifluoromethyl.
Favourably, R3 represents hydrogen, C1-6 alkyl, C3-7 heterocycloalkenyl (optionally substituted by one or two methyl groups), cyano, —CO2Ra, —CONRbRc, —CON(ORb)Rc, —CON(Rd)NRbRc, —C(═NRe)NRbRc or —CON(Rd)C(═NRe)NRbRc, in which Ra, Rb, Rc, Rd and Re are as defined above.
Suitably, R3 represents hydrogen, —CO2Ra, —CONRbRc, —CON(ORb)Rc or —CONHNRbRc, in which Ra, Rb and Rc are as defined above.
In one embodiment, R3 represents hydrogen. In another embodiment, R3 represents C1-6 alkyl, especially methyl. In another embodiment, R3 represents C3-7 heterocycloalkenyl (optionally substituted by one or two methyl groups), e.g. 4,4-dimethyl-4,5-dihydro-1H-imidazol-2-yl. In another embodiment, R3 represents cyano. In another embodiment, R3 represents —CO2Ra, in which Ra is as defined above. In a further embodiment, R3 represents —CONRbRc, in which Rb and Rc are as defined above. In a still further embodiment, R3 represents —CON(ORb)Rc, in which Rb and Rc are as defined above. In an additional embodiment, R3 represents —CONHNRbRc, in which Rb and Rc are as defined above. In another additional embodiment, R3 represents —C(═NH)NRbRc, in which Rb and Rc are as defined above. In a further additional embodiment, R3 represents —CONHC(═NH)NRbRc, in which Rb and Rc are as defined above.
Suitable values of R4a and/or R4b include hydrogen, halogen (especially fluoro or chloro) and C1-6 alkyl (especially methyl).
Preferably, R4a is attached at the 2-position relative to the anilino nitrogen atom.
Suitably, R4a represents halogen. In one embodiment, R4a is fluoro. In another embodiment, R4a is chloro.
Typically, R4b may be attached at the 6-position relative to the anilino nitrogen atom.
Suitably, R4b is hydrogen.
Suitably, R5 represents halogen, nitro, cyano, C2-6 alkynyl, hydroxy(C1-6)alkyl or formyl. Typically, R5 represents halogen, nitro, hydroxy(C1-6)alkyl or formyl.
In one embodiment, R5 represents halogen, especially bromo or iodo, particularly iodo. In another embodiment, R5 represents nitro. In another embodiment, R5 represents cyano. In another embodiment, R5 represents C1-6 alkyl, especially methyl. In another embodiment, R5 represents C2-6 alkynyl, especially ethynyl. In a further embodiment, R5 represents hydroxy(C1-6)alkyl, especially hydroxymethyl. In an additional embodiment, R5 represents formyl.
Suitably, R6 represents hydrogen or C1-6 alkyl. In one embodiment, R6 represents hydrogen. In another embodiment, R6 represents C1-6 alkyl, especially methyl.
In one embodiment, R7 represents hydrogen. In another embodiment, R7 represents C1-6 alkyl, especially methyl.
In one embodiment, R8 represents hydrogen. In another embodiment, R8 represents C1-6 alkyl, especially methyl.
One sub-class of compounds according to the invention is represented by the compounds of formula (IIA), and pharmaceutically acceptable salts and solvates thereof:
wherein
-X-, R3, R4a, R4b and R5 are as defined above;
R11 represents hydrogen or optionally substituted C1-6 alkyl; and
R12 represents hydrogen; or C1-6 alkyl, C3-7 cycloalkyl, C3-7 cycloalkyl(C1-6)alkyl, aryl, aryl(C1-6)alkyl, C3-7 heterocycloalkyl, C3-7 heterocycloalkyl(C1-6)alkyl, heteroaryl or heteroaryl(C1-6)alkyl, any of which groups may be optionally substituted by one or more substituents; or
R11 and R12, when taken together with the carbon atom to which they are both attached, represent C3-7 cycloalkyl or C3-7 heterocycloalkyl, either of which groups may be optionally substituted by one or more substituents.
Where R11 and/or R12 in the compounds of formula (IIA) above is stated to be optionally substituted, this group may be unsubstituted, or substituted by one or more substituents. Typically, R11 and/or R12 will be unsubstituted, or substituted by one or two substitutents. Suitably, R11 and/or R12 will be unsubstituted or monosubstituted.
Suitably, R11 represents hydrogen or unsubstituted C1-6 alkyl.
Suitably, R12 represents hydrogen; or C1-6 alkyl, aryl or heteroaryl, any of which groups may be optionally substituted by one or more substituents. Particular values of R12 include hydrogen and unsubstituted C1-6 alkyl.
Examples of typical substituents on R11 and/or R12 include halogen, cyano, nitro, C1-6 alkyl, trifluoromethyl, hydroxy, C1-6 alkoxy, difluoromethoxy, trifluoromethoxy, aryloxy, C1-6 alkylthio, C1-6 alkylsulphonyl, amino, C1-6 alkylamino, di(C1-6)alkylamino, C2-6 alkylcarbonylamino, C2-6 alkoxycarbonylamino, C1-6 alkylsulphonylamino, formyl, C2-6 alkylcarbonyl, carboxy, C2-6 alkoxycarbonyl, aminocarbonyl, C1-6 alkylamino-carbonyl, di(C1-6)alkylaminocarbonyl, aminosulphonyl, C1-6 alkylaminosulphonyl and di(C1-6)alkylaminosulphonyl; especially halogen, C1-6 alkoxy or C1-6 alkylthio.
Examples of particular substituents on R11 and/or R12 include fluoro, chloro, bromo, cyano, nitro, methyl, trifluoromethyl, hydroxy, methoxy, difluoromethoxy, trifluoromethoxy, phenoxy, methylthio, methylsulphonyl, amino, methylamino, dimethylamino, acetylamino, methoxycarbonylamino, methylsulphonylamino, formyl, acetyl, carboxy, methoxycarbonyl, aminocarbonyl, methylaminocarbonyl, dimethylaminocarbonyl, aminosulphonyl, methylaminosulphonyl and dimethylaminosulphonyl; especially chloro, methoxy or methylthio.
Typical values of R11 include hydrogen and methyl. In one embodiment, R11 is hydrogen. In another embodiment, R11 is methyl.
Typical values of R12 include hydrogen, methyl, n-propyl, isopropyl, phenyl, chlorophenyl, methoxyphenyl, methylthiophenyl and furyl, especially hydrogen or methyl. In one embodiment, R12 is hydrogen. In another embodiment, R12 is methyl.
Alternatively, R11 and R12 may together form an optionally substituted spiro linkage. Thus, R11 and R12, when taken together with the carbon atom to which they are both attached, may represent C3-7 cycloalkyl or C3-7 heterocycloalkyl, either of which groups may be unsubstituted, or substituted by one or more, typically by one or two, substituents. In this context, R11 and R12, when taken together with the carbon atom to which they are both attached, may suitably represent an optionally substituted cyclopentyl, cyclohexyl, pyrrolidine or piperidine ring, especially cyclopentyl or cyclohexyl.
One particular sub-group of the compounds of formula (IIA) is represented by the compounds of formula (IIB), and pharmaceutically acceptable salts and solvates thereof:
wherein
R3, R6, R11 and R12 are as defined above;
R14 represents halogen; and
R15 represents halogen, nitro, cyano, C2-6 alkynyl, hydroxy(C1-6)alkyl or formyl.
In one specific embodiment, R14 is fluoro. In another specific embodiment, R14 is chloro.
Typically, R15 represents halogen, nitro, hydroxy(C1-6)alkyl or formyl.
In one embodiment, R15 represents halogen, especially iodo. In another embodiment, R15 represents nitro. In another embodiment, R15 represents cyano. In another embodiment, R15 represents C2-6 alkynyl, especially ethynyl. In a further embodiment, R15 represents hydroxy(C1-6)alkyl, especially hydroxymethyl. In an additional embodiment, R15 represents formyl.
Another sub-group of the compounds of formula (IIA) is represented by the compounds of formula (IIC), and pharmaceutically acceptable salts and solvates thereof:
wherein
R3, R11 and R12, R14 and R15 are as defined above.
A further sub-group of the compounds of formula (IIA) is represented by the compounds of formula (IID), and pharmaceutically acceptable salts and solvates thereof:
wherein
R3, R6, R11, R12, R14 and R15 are as defined above.
Specific novel compounds in accordance with the present invention include each of the compounds whose preparation is described in the accompanying Examples, and pharmaceutically acceptable salts and solvates thereof.
The present invention also provides a pharmaceutical composition which comprises a compound of formula (I) as defined above, or a pharmaceutically acceptable salt or solvate thereof, in association with one or more pharmaceutically acceptable carriers.
Pharmaceutical compositions according to the invention may take a form suitable for oral, buccal, parenteral, nasal, topical, ophthalmic or rectal administration, or a form suitable for administration by inhalation or insufflation.
For oral administration, the pharmaceutical compositions may take the form of, for example, tablets, lozenges or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g. pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methyl cellulose); fillers (e.g. lactose, microcrystalline cellulose or calcium hydrogenphosphate); lubricants (e.g. magnesium stearate, talc or silica); disintegrants (e.g. potato starch or sodium glycollate); or wetting agents (e.g. sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents, emulsifying agents, non-aqueous vehicles or preservatives. The preparations may also contain buffer salts, flavouring agents, colouring agents or sweetening agents, as appropriate.
Preparations for oral administration may be suitably formulated to give controlled release of the active compound.
For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.
The compounds of formula (I) may be formulated for parenteral administration by injection, e.g. by bolus injection or infusion. Formulations for injection may be presented in unit dosage form, e.g. in glass ampoules or multi-dose containers, e.g. glass vials. The compositions for injection may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising, preserving and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile pyrogen-free water, before use.
In addition to the formulations described above, the compounds of formula (I) may also be formulated as a depot preparation. Such long-acting formulations may be administered by implantation or by intramuscular injection.
For nasal administration or administration by inhalation, the compounds according to the present invention may be conveniently delivered in the form of an aerosol spray presentation for pressurised packs or a nebuliser, with the use of a suitable propellant, e.g. dichlorodifluoromethane, fluorotrichloromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas or mixture of gases.
The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack or dispensing device may be accompanied by instructions for administration.
For topical administration the compounds according to the present invention may be conveniently formulated in a suitable ointment containing the active component suspended or dissolved in one or more pharmaceutically acceptable carriers. Particular carriers include, for example, mineral oil, liquid petroleum, propylene glycol, polyoxyethylene, polyoxypropylene, emulsifying wax and water. Alternatively, the compounds according to the present invention may be formulated in a suitable lotion containing the active component suspended or dissolved in one or more pharmaceutically acceptable carriers. Particular carriers include, for example, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, benzyl alcohol, 2-octyldodecanol and water.
For ophthalmic administration the compounds according to the present invention may be conveniently formulated as microionized suspensions in isotonic, pH-adjusted sterile saline, either with or without a preservative such as a bactericidal or fungicidal agent, for example phenylmercuric nitrate, benzylalkonium chloride or chlorhexidine acetate. Alternatively, for ophthalmic administration compounds may be formulated in an ointment such as petrolatum.
For rectal administration the compounds according to the present invention may be conveniently formulated as suppositories. These can be prepared by mixing the active component with a suitable non-irritating excipient which is solid at room temperature but liquid at rectal temperature and so will melt in the rectum to release the active component. Such materials include, for example, cocoa butter, beeswax and polyethylene glycols.
The quantity of a compound of the invention required for the prophylaxis or treatment of a particular condition will vary depending on the compound chosen and the condition of the patient to be treated. In general, however, daily dosages may range from around 10 ng/kg to 1000 mg/kg, typically from 100 ng/kg to 100 mg/kg, e.g. around 0.01 mg/kg to 40 mg/kg body weight, for oral or buccal administration, from around 10 ng/kg to 50 mg/kg body weight for parenteral administration, and from around 0.05 mg to around 1000 mg, e.g. from around 0.5 mg to around 1000 mg, for nasal administration or administration by inhalation or insufflation.
The compounds of formula (I) above may be prepared by a process which comprises reacting a compound of formula (III) with a compound of formula (IV):
wherein R1, R2, R3, R4a, R4b, R5 and X are as defined above, and L1 represents a suitable leaving group.
The leaving group L1 is typically a halogen atom, e.g. bromo.
The reaction is conveniently effected at an elevated temperature in a suitable solvent, e.g. N,N-dimethylformamide, typically under basic conditions, e.g. in the presence of a base such as cesium carbonate.
By way of example, the intermediates of formula (IV) above wherein R3 is ethoxycarbonyl may be prepared by a process which comprises reacting the product formed by reacting ethyl acetoacetate and sodium ethoxide with a compound of formula (V):
wherein R4a, R4b and R5 are as defined above.
The reaction is conveniently effected by stirring the reactants in a suitable solvent, e.g. a lower alkanol such as ethanol.
Similarly, the intermediates of formula (IV) above wherein R3 is cyano may be prepared by a process which comprises reacting compound (V) with acetonitrile, typically in the presence of a strong base such as sodium hexamethyldisilazide.
The intermediates of formula (V) above may be prepared by reacting a compound of formula (VI):
wherein R4a, R4b and R5 are as defined above; with thiophosgene.
The reaction is conveniently effected in a suitable solvent, typically a mixture of chloroform and water.
In an alternative procedure, the compounds of formula (I) above may be prepared by a process which comprises reacting a compound of formula (VII) with a compound of formula (VIII):
wherein R1, R2, R3, R4a, R4b, R5 and X are as defined above.
The reaction is conveniently effected, at an elevated temperature if required, in a suitable solvent, e.g. N,N-dimethylformamide, typically under basic conditions, e.g. in the presence of a base such as cesium carbonate.
By way of example, the intermediates of formula (VII) above wherein R3 represents —CO2Ra may be prepared by reacting a compound of formula N≡C—CH2—CO2Ra with the appropriate compound of formula (IX):
wherein X, R1, R2 and Ra are as defined above; in the presence of sulphur.
The reaction is conveniently effected at an elevated temperature in a suitable solvent, e.g. a lower alkanol such as ethanol, typically under basic conditions, e.g. in the presence of morpholine.
In another procedure, the compounds of formula (I) above may be prepared by a process which comprises reacting a compound of formula (VI) as defined above with a compound of formula (X):
wherein X, R1, R2 and R3 are as defined above.
The reaction is conveniently effected in the presence of a strong base such as sodium hexamethyldisilazide.
The intermediates of formula (X) above may be prepared from the precursors of formula (VII) as defined above by a multi-stage procedure which comprises the following steps: (i) diazotisation/bromination by treatment with tert-butyl nitrite and copper(II) bromide; (ii) treatment of the bromo derivative thereby obtained with dimethyl disulphide, typically in the presence of a strong base such as tert-butyllithium; and subsequently (iii) oxidation of the methylthio derivative thereby obtained, typically with an oxidising agent such as 3-chloroperoxybenzoic acid, to afford the desired compound of formula (X).
Where they are not commercially available, the starting materials of formula (III), (VI), (VIII) and (IX) may be prepared by methods analogous to those described in the accompanying Examples, or by standard methods well known from the art.
It will be understood that any compound of formula (I) initially obtained from any of the above processes may, where appropriate, subsequently be elaborated into a further compound of formula (I) by techniques known from the art. By way of example, a compound of formula (IA), (IB), (IC) or (ID) wherein Y is oxygen may be converted into the corresponding compound wherein Y is sulphur by treatment with Lawesson's Reagent (i.e. 2,4-bis(4-methoxyphenyl)-1,3-dithia-2,4-diphosphetane-2,4-disulphide). A compound of formula (IB) wherein Y is oxygen may be converted into the corresponding compound of formula (ID) by treatment with hydroxylamine-O-sulfonic acid, typically in the presence of formic acid at an elevated temperature. A compound of formula (IB) wherein Y is oxygen may be converted into the corresponding compound of formula (IE) by treatment with a reducing agent such as lithium aluminium hydride. A compound of formula (IB) wherein Y is oxygen may be converted into the corresponding compound of formula (IF) by treatment with a hydroxylamine derivative of formula H2N—OR7. A compound of formula (IB) wherein Y is oxygen may be converted into the corresponding compound of formula (IG) by treatment with hydrazine hydrate. A compound of formula (IF) may be converted into the corresponding compound of formula (IC) by treatment with p-toluenesulphonyl chloride, typically in the presence of pyridine at an elevated temperature. A compound of formula (IB) wherein Y is oxygen and R1 is hydrogen may be converted into the corresponding compound wherein R1 is methyl by treatment with a methyl halide, e.g. iodomethane, in the presence of a strong base, e.g. lithium diisopropylamide. A compound of formula (IC) wherein Y is sulphur may be converted into the corresponding compound wherein Y is NH by treatment with dimethyl sulphate or iodomethane, followed by treatment of the methylthio-substituted cyclic imine thereby obtained with ammonia, typically at elevated temperature and pressure, or with ammonium acetate, typically at elevated temperature.
A compound of formula (IC) wherein R6 represents hydrogen may be converted into the corresponding compound wherein R6 represents C1-6 alkyl by treatment with a trialkylsilyl halide, e.g. trimethylsilyl chloride or tert-butyldimethylsilyl chloride, in the presence of a base, e.g. sodium hydride, followed by treatment with a C1-6 alkyl halide, e.g. iodomethane, in the presence of a base, e.g. sodium hydride.
A compound of formula (I) wherein R3 represents —CO2Ra in which R1 is other than hydrogen may be saponified to give the corresponding compound in which R3 represents —CO2H by treatment with a base such as lithium hydroxide; more prolonged treatment with lithium hydroxide gives rise to the decarboxylated product in which R3 represents hydrogen. A compound of formula (I) wherein R3 represents —CO2Ra may be converted into the corresponding compound wherein R3 represents —CONH2 by treatment with ammonia, typically at elevated temperature and optionally also at elevated pressure. A compound of formula (I) wherein R3 represents —CO2H may be converted into the corresponding compound wherein R3 represents —CONRbRc, —CON(ORb)Rc or —CON(Rd)C(═NRe)NRbRc by treatment with the appropriate amine of formula H—NRbRc, H—N(ORb)Rc or H—N(Rd)C(═NRe)NRbRc respectively and a condensing agent such as 1,1′-carbonyldiimidazole (CDI) or 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide (EDC), typically in the presence of 4-methylmorpholine (NMM) and 1-hydroxybenzotriazole (HOBT). Alternatively, a compound of formula (I) wherein R3 represents —CO2H may be converted into the corresponding compound wherein R3 represents —CONRbRc, —CON(ORb)Rc or —CON(Rd)NRbRc by a two-stage procedure which comprises (i) treatment with pentafluorophenol and a condensing agent such as EDC, typically in the presence of NMM and HOBT; and (ii) reaction of the pentafluorophenyl ester thereby obtained with the appropriate amine of formula H—NRbRc, H—N(ORb)Rc or H—N(Rd)NRbRc respectively, typically in the presence of an organic base such as triethylamine.
A compound of formula (I) wherein R3 represents —CO2H may be converted into the corresponding compound wherein R3 represents —CO2Ra by a two-stage procedure which comprises (i) treatment with pentafluorophenol and a condensing agent such as EDC, typically in the presence of NMM and HOBT; and (ii) reaction of the pentafluorophenyl ester thereby obtained with the appropriate alcohol of formula RaOH, typically in the presence of an organic base such as triethylamine.
A compound of formula (I) wherein R3 represents —CO2H may be converted into the corresponding compound wherein R3 represents —CONRbRc by a two-stage procedure which comprises (i) treatment with tetrafluorophenol resin and a condensing agent such as 1,3-diisopropylcarbodiimide, typically in the presence of 4-(dimethylamino)pyridine; and (ii) reaction of the tetrafluorophenyl ester functionalised resin thereby obtained with the appropriate amine of formula H—NRbRc.
A compound of formula (I) wherein R3 represents —CO2Ra (e.g. ethoxycarbonyl) may be converted into the corresponding compound wherein R3 represents methyl by treatment with a reducing agent such as diisobutylaluminium hydride.
A compound of formula (I) wherein R3 represents —CO2Ra (e.g. ethoxycarbonyl) may be converted into the corresponding compound wherein R3 represents —CONRbRc by treatment with the appropriate amine of formula H—NRbRc in the presence of trimethylaluminium. Similarly, a compound of formula (I) wherein R3 represents cyano may be converted into the corresponding compound wherein R3 represents —C(═NH)NRbRc by treatment with the appropriate amine of formula H—NRbRc in the presence of trimethylaluminium. A compound of formula (I) wherein R3 represents cyano may be converted into the corresponding compound wherein R3 represents 4,4-dimethyl-4,5-dihydro-1H-imidazol-2-yl in a single step by treatment with 1,2-diamino-2-methylpropane in the presence of trimethylaluminium.
A compound of formula (I) wherein R3 represents cyano may be converted into the corresponding compound wherein R3 represents —CONH2 by treatment with hydroxylamine.
A compound of formula (I) wherein R3 contains a NH functionality may be converted into the corresponding compound wherein R3 contains a N-methyl functionality by treatment with formaldehyde in the presence of a suitable reducing agent, e.g. sodium cyanoborohydride.
A compound of formula (I) wherein R5 represents formyl may be converted into the corresponding compound wherein R5 represents hydroxymethyl by treatment with a suitable reducing agent, e.g. sodium borohydride.
A compound of formula (I) wherein R5 represents iodo may be converted into the corresponding compound wherein R5 represents ethynyl by treatment at an elevated temperature with (trimethylsilyl)acetylene and a transition metal catalyst, e.g. bis(triphenylphosphine)palladium(II) dichloride, typically in the presence of copper(I) iodide and a base such as diisopropylamine.
Where a mixture of products is obtained from any of the processes described above for the preparation of compounds according to the invention, the desired product can be separated therefrom at an appropriate stage by conventional methods such as preparative HPLC; or column chromatography utilising, for example, silica and/or alumina in conjunction with an appropriate solvent system.
Where the above-described processes for the preparation of the compounds according to the invention give rise to mixtures of stereoisomers, these isomers may be separated by conventional techniques. In particular, where it is desired to obtain a particular enantiomer of a compound of formula (I) this may be produced from a corresponding mixture of enantiomers using any suitable conventional procedure for resolving enantiomers. Thus, for example, diastereomeric derivatives, e.g. salts, may be produced by reaction of a mixture of enantiomers of formula (I), e.g. a racemate, and an appropriate chiral compound, e.g. a chiral base. The diastereomers may then be separated by any convenient means, for example by crystallisation, and the desired enantiomer recovered, e.g. by treatment with an acid in the instance where the diastereomer is a salt. In another resolution process a racemate of formula (I) may be separated using chiral HPLC. Moreover, if desired, a particular enantiomer may be obtained by using an appropriate chiral intermediate in one of the processes described above. Alternatively, a particular enantiomer may be obtained by performing an enantiomer-specific enzymatic biotransformation, e.g. an ester hydrolysis using an esterase, and then purifying only the enantiomerically pure hydrolysed acid from the unreacted ester antipode. Chromatography, recrystallisation and other conventional separation procedures may also be used with intermediates or final products where it is desired to obtain a particular geometric isomer of the invention.
During any of the above synthetic sequences it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J. F. W. McOmie, Plenum Press, 1973; and T. W. Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 3rd edition, 1999. The protecting groups may be removed at any convenient subsequent stage utilising methods known from the art.
The following Examples illustrate the preparation of compounds according to the invention.
The compounds in accordance with this invention potently inhibit the activity of human MEK enzyme.
MEK1 activity was measured in a cascade assay initiated by active Raf, via activation of MEK, Erk2 and subsequent phosphorylation of fluorescein-labelled Erk-tide substrate in an assay based on fluorescence polarisation (IMAP). The assay was carried out in 20 mM Tris+5 mM MgCl2+2 mM DL-dithiothreitol+0.01% Tween 20 pH 7.2, containing 1.5 nM unactive MEK, 100 nM unactive Erk and 200 nM Erk-tide (all concentrations are final concentrations). Compounds, or DMSO controls, were tested at a final concentration of 2% DMSO, and the assay initiated in the presence of 5 μM ATP by addition of 1.25 nM active Raf in assay buffer. After 20 min at r.t., stop solution was added followed by IMAP binding beads, the assay mixture was then incubated for 90 min at r.t. (with shaking) and then read on a Molecular Devices UL HT reader.
When tested in the above assay, the compounds of the accompanying Examples were all found to inhibit human MEK enzyme with IC50 values of 10 μM or better.
All NMRs were obtained either at 300 MHz or 400 MHz.
The LC-MS system used comprises a Waters Alliance 2795 HT quaternary HPLC, Waters 996 Photo Diode Array (PDA) detector and Waters ZQ 4000 single quadrupole mass spectrometer. The ZQ can acquire data simultaneously in positive and negative electrospray ionisation modes.
Data were acquired in a full scan from 100 to 1000 m/z.
The reverse phase separation was carried out on a Gemini C18 from Phenomenex 50×4.6 mm with 5 μm silica.
Solvent A: 90% 10 mM NH4CO2 in water/0.1% formic acid/10% CH3CN
Solvent B: 90% CH3CN/0.1% formic acid/10% 10 mM NH4CO2 in water
The aqueous solvent was approximately pH 3.2.
Hydroxylamine hydrochloride (39.66 g, 0.57 mol) was added to a solution of dimedone (80 g, 0.57 mol) in MeOH (500 mL) and the reaction was heated to reflux for 4 h. The reaction was concentrated in vacuo to give an orange oil. The resulting oil was dissolved in MeCN (500 mL) and triethylamine (85.6 mL, 0.62 mol) added. The reaction was cooled to 0° C. and a solution of p-toluenesulphonyl chloride (112.0 g, 0.59 mol) in MeCN (600 mL) was added slowly dropwise and the reaction stirred at r.t. for 1 h. Water (32 mL) was added and the reaction heated to 60° C. for 18 h. The reaction was cooled, K2CO3 (172 g, 1.24 mol) added, and the suspension was stirred at r.t. for 2 h. The mixture was filtered and the resulting filtrate concentrated in vacuo. The residue was dissolved in DCM (800 mL) and washed with water (100 mL). The combined organic extracts were dried (MgSO4) and filtered through silica gel (100 g). The resulting filtrate was concentrated in vacuo to give a brown solid. The solid was dissolved in 1:1 THF/heptane (360 mL) and heated to 50° C. for 1.5 h. The mixture was cooled, and the resulting precipitate filtered and dried in vacuo to give the title compound as a beige solid (32.1 g, 36%). δH (DMSO-d6) 7.89 (1H, br s), 3.43 (2H, s), 3.08 (2H, d, J 6.3 Hz), 2.37 (2H, s), 0.90 (6H, s). LCMS (ES+) RT 1.87 minutes, 156 (M+H)+.
N-Bromosuccinimide (11.5 g, 64.5 mmol) was added slowly to a solution of
Intermediate 1 (10.0 g, 64.5 mmol) and NaHSO4 (1.94 g, 16.1 mmol) in THF (350 mL) at 0° C., and the reaction was warmed to r.t. for 1 h. Aqueous NaHCO3 (300 mL) was added to the reaction, and the mixture extracted with DCM (3×100 mL). The combined organic extracts were dried (MgSO4), and concentrated in vacuo to give a white solid. The solid was triturated with isopropyl ether and the resulting precipitate filtered and dried in vacuo to give the title compound as a white solid (11.18 g, 74%). δH (DMSO-d6) 8.37 (1H, m), 5.74 (1H, s), 3.29 (1H, dd, J 15.1, 6.2 Hz), 2.50-2.70 (2H, m), 2.32 (1H, d, J 11.9 Hz), 0.99 (3H, s), 0.86 (3H, s). LCMS (ES+) RT 2.17 minutes, 236 (M+H)+.
Thiophosgene (17.8 ml, 232 mmol) was added to a rapidly stirred mixture of 2-fluoro-4-iodoaniline (50.0 g, 211 mmol) in CHCl3 (500 ml) and water (300 ml). The mixture was stirred at room temperature for 4 hours. The organic phase was separated, washed with saturated sodium bicarbonate solution, dried (Na2SO4), filtered and the volatiles removed in vacuo to give the title compound as an off-white crystalline solid (51.7 g, 88%). δH (DMSO-d6) 7.87 (1H, dd, J 1.8, 9.5 Hz), 7.63 (1H, ddd, J 1.0, 1.8, 8.4 Hz), 7.25 (1H, dd, J 8.2, 8.4 Hz).
Sodium metal (2 g, 87 mmol) was dissolved in ethanol (250 mL), treated with ethyl acetoacetate (10.72 g, 82.5 mmol) and the reaction stirred for 15 min. Intermediate 3 (23 g, 82.5 mmol) was added portionwise and the reaction stirred at r.t. for 4 h. The reaction mixture was poured onto 2M HCl and extracted into ethyl acetate, dried (Na2SO4), filtered and concentrated in vacuo, yielding a red solid. δH (DMSO-d6) 11.62 (1H, s), 7.78 (1H, dd, J 9.7, 1.8 Hz), 7.63 (1H, m), 7.40 (1H, t, J 8.1 Hz), 4.03 (2H, q, J 7.1 Hz), 3.89 (2H, s), 1.21 (3H, t, J 7.1 Hz). LCMS (ES+) RT 2.69 minutes, 366 (M+H)+.
1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide (1.78 g, 9.28 mmol) was added to a mixture of 4-methylmorpholine (1.78 g, 16.88 mmol), 1-hydroxybenzotriazole (1.14 g, 8.44 mmol), pentafluorophenol (1.55 g, 8.44 mmol) and Example 2 (4.0 g, 8.44 mmol) in DCM/DMF (10:1 mixture; 300 mL) and stirred at r.t. for 18 h. Brine (100 mL) was added to the reaction, and the mixture was extracted with DCM (3×100 mL). The combined organic extracts were dried (MgSO4) and concentrated in vacuo. The residual DMF was azeotroped with heptane. The crude product was triturated with MeCN to give the title compound as a cream solid (3.2 g, 59%). δH (DMSO, d6) 9.77 (1H, s), 8.05 (1H, t, J 5.0 Hz), 7.84 (1H, dd, J 9.9, 1.8 Hz), 7.69 (1H, dd, J 8.4, 0.9 Hz), 7.44 (1H, t, J 8.4 Hz), 2.92 (2H, s), 2.88 (2H, d, J 5.0 Hz), 0.98 (6H, s). LCMS (ES+) RT 3.76 minutes, 641 (M+H)+.
Sulphur (2.50 g, 78.1 mmol) and ethyl cyanoacetate (8.3 mL, 78.1 mmol) were added to a solution of Intermediate 1 (10.1 g, 65.1 mmol) in EtOH (30 mL) and the reaction heated to 45° C. for 0.5 h. Morpholine (6.8 mL, 78.1 mmol) was added slowly dropwise and the reaction mixture heated to 65° C. for 18 h. The reaction was cooled to 0° C. and the precipitate filtered and dried in vacuo to give the title compound as a white solid (9.4 g, 51%). δH (DMSO-d6) 7.73 (1H, t, J 5.1 Hz), 7.69 (2H, br s), 4.21 (2H, q, J 7.1 Hz), 2.82 (2H, s), 2.79 (2H, d, J 5.2 Hz), 1.27 (3H, t, J 7.1 Hz), 0.94 (6H, s). LCMS (ES+) RT 2.58 minutes, 283 (M+H)+.
1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide (98 mg, 0.51 mmol) was added to a mixture of 4-methylmorpholine (112 μL, 1.02 mmol), 1-hydroxybenzotriazole (69 mg, 0.51 mmol), (S)-(+)-(2,2-dimethyl-[1,3]dioxolan-4-yl)methylamine (73 mg, 0.56 mmol) and Example 5 (250 mg, 0.51 mmol) in DMF (5 mL) and stirred at r.t. for 18 h. Brine (50 mL) was added to the reaction, and the mixture was extracted with DCM (3×10 mL). The combined organic extracts were dried (MgSO4) and concentrated in vacuo. The residual DMF was azeotroped with heptane. The crude product was purified by chromatography (silica, 0-30% EtOAc in DCM) to give the title compound as a cream solid (130 mg, 42%). δH (CDCl3) 10.51 (1H, s), 7.64 (1H, d, J 2.0 Hz), 7.48 (1H, dd, J 8.6, 2.0 Hz), 7.35 (1H, d, J 8.6 Hz), 6.30 (1H, br s), 6.08 (1H, t, J 5.3 Hz), 4.31-4.23 (1H, m), 4.08-4.03 (1H, m), 3.81-3.73 (1H, m), 3.63 (1H, dd, J 8.5, 6.0 Hz), 3.38-3.29 (1H, m), 2.98 (2H, d, J 4.7 Hz), 2.78 (2H, s), 1.36 (3H, s), 1.24 (3H, s) 1.06 (6H, s). LCMS (ES+) RT 3.19 minutes, 604 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and piperazine-1-carboxylic acid tert-butyl ester (104 mg, 0.56 mmol) by the method of Intermediate 7. The title compound was obtained as a cream solid (100 mg, 30%). δH (CDCl3) 7.61 (1H, d, J 2.0 Hz), 7.43 (1H, dd, J 8.6, 2.0 Hz), 7.39 (1H, br s), 7.23 (1H, d, J 8.6 Hz), 6.46 (1H, t, J 4.8 Hz), 3.48-3.33 (6H, m), 3.28-3.17 (2H, m), 2.97 (2H, d, J 4.9 Hz), 2.52 (2H, s), 1.39 (9H, s), 0.98 (6H, s). LCMS (ES+) RT 3.15 minutes, 659 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and O-(2,2-dimethyl-[1,3]dioxolan-4-ylmethyl)hydroxylamine (prepared according to the procedure described in WO 02/06213; 79 mg, 0.54 mmol) by the method of Intermediate 7. The title compound was obtained as a yellow solid (74 mg, 23%). δH (MeOH-d4) 7.77 (1H, d, J 2.9 Hz), 7.70-7.53 (4H, m), 7.19 (1H, d, J 8.6 Hz), 4.36-4.30 (1H, m), 4.17-4.05 (2H, m), 3.92-3.75 (2H, m), 3.01 (2H, d, J 4.2 Hz), 2.81 (2H, s), 1.38 (3H, s), 1.33 (3H, s), 1.08 (6H, s). LCMS (ES+) RT 3.12 minutes, 620 (M+H)+.
To a solution of (S)-2,2-dimethyl-1,3-dioxolane-4-acetamide (2.0 g, 12.56 mmol) in THF (60 mL) at 0° C. was added lithium aluminium hydride (1.67 g, 43.95 mmol). The reaction was stirred at room temperature for 2 h. The reaction was quenched by slow addition of 1.7 mL of water, followed by 1.7 mL of aqueous KOH (15% w/v), followed by approximately 3.5 mL of water. The resulting precipitate was removed by filtration, and the filtrate concentrated in vacuo. The title compound was obtained as a yellow oil (1.41 g, 77%) and used crude without further purification.
Prepared from Example 5 (250 mg, 0.51 mmol) and Intermediate 10 (222 mg, 1.53 mmol) by the method of Intermediate 7. The title compound was obtained as an off-white solid (90 mg, 28%). δH (CDCl3) 10.53-10.36 (1H, m), 7.73 (1H, d, J 0.7 Hz), 7.56 (1H, d, J 8.6 Hz), 7.43 (1H, d J 8.6 Hz), 6.44-6.31 (2H, m), 4.33-4.18 (1H, m), 4.17-4.00 (1H, m), 3.87-3.65 (1H, m), 3.65-3.51 (2H, m), 3.07 (2H, s), 2.84 (2H, s), 2.07-1.94 (3H, m), 1.86-1.74 (1H, m), 1.41 (3H, s), 1.35 (3H, s), 1.12 (6H, s). LCMS (ES+) RT 3.43 minutes, 618 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and 4-(aminomethyl)piperidine-1-carboxylic acid tert-butyl ester (164 mg, 0.76 mmol) by the method of Intermediate 7. The title compound was obtained as an off-white solid (117 mg, 33%). δH (CDCl3) 10.05 (1H, s), 7.62 (1H, d, J 2.0 Hz), 7.44 (1H, dd, J 8.6 2.0 Hz), 7.23 (1H, d, J 8.6 Hz), 6.10-6.00 (2H, br m), 4.10-4.04 (1H, br m), 3.34-3.29 (2H, br m), 2.95 (2H, d, J 5.2 Hz), 2.71 (2H, s), 2.70-2.64 (2H, br m), 1.75-1.56 (4H, br m), 1.38 (9H, s), 1.15-1.06 (2H, br m), 1.02 (6H, s). LCMS (ES+) RT 3.82 minutes, 687 (M+H)+.
To a suspension of Example 4 (478 mg, 1.0 mmol) in THF (5 mL) under nitrogen was added sodium hydride (44 mg, 1.1 mmol). The solution was stirred for 20 minutes before tert-butyldimethylsilyl chloride (109 mg, 1.0 mmol), was added and stirring continued for a further 15 minutes. A second portion of sodium hydride (44 mg, 1.1 mmol) was added and the reaction stirred for 15 minutes. Methyl iodide (142 mg, 1.0 mmol) was added and the reaction stirred at room temperature for 24 hours. The reaction mixture was poured into brine (100 mL) and the mixture extracted with ethyl acetate (3×100 mL). The combined organic extracts were dried (Na2SO4), filtered and the solvents removed in vacuo. The crude residue was purified by column chromatography (SiO2, 0-10% ethyl acetate in dichloromethane) to give the title compound as a pale cream solid (210 mg, 43%). δH (CDCl3) 10.87 (1H, s), 7.77 (1H, d, J 2.1 Hz), 7.61 (1H, m), 7.56 (1H, m), 4.42 (2H, q, J 7.1 Hz), 3.19 (3H, s), 3.08 (2H, s), 2.99 (2H, s), 1.44 (3H, t, J 7.1 Hz), 1.09 (6H, s). LCMS (ES+) RT 4.60 minutes, 533 (M+H)+.
Intermediate 13 (130 mg, 0.24 mmol) was dissolved in THF (5 mL) and a solution of lithium hydroxide (21 mg, 0.48 mmol) in water (1 mL) added. The mixture was heated to 75° C. for 18 hours. The volatiles were removed in vacuo and the residue treated with 10% aqueous citric acid solution. The resultant precipitate was filtered and dried under suction to give the title compound as a cream solid (125 mg, quant.). LCMS (ES−) RT 2.71 minutes, 503 (M+H)−.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (R)-3-aminopiperidine-1-carboxylic acid tert-butyl ester (78 mg, 0.39 mmol) by the method of Example 19. The title compound was obtained as a yellow solid (130 mg, 51%). δH (CDCl3) 10.48 (1H, s), 7.46 (2H, dd, J 10.1, 1.6 Hz), 7.36 (1H, t, J 8.3 Hz), 6.35 (1H, br s), 6.04 (1H, d, J 7.1 Hz), 4.23-4.20 (1H, m), 3.60-3.46 (3H, m), 3.34-3.30 (1H, m), 3.04 (2H, d, J 4.9 Hz), 2.80 (2H, s), 1.92-1.80 (2H, m), 1.67-1.61 (2H, m), 1.45 (9H, s), 1.11 (6H, d, J 3.2 Hz). LCMS (ES+) RT 3.75 minutes, 657 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (S)-3-aminopiperidine-1-carboxylic acid tert-butyl ester (78 mg, 0.39 mmol) by the method of Example 19. The title compound was obtained as a yellow solid (172 mg, 67%). δH (CDCl3) 10.51 (1H, s), 7.48-7.44 (2H, m), 7.37 (1H, t, J 8.3 Hz), 6.37 (1H, br s), 5.99 (1H, d, J 7.1 Hz), 4.23-4.17 (1H, m), 3.60-3.52 (3H, m), 3.32-3.26 (1H, m), 3.06 (2H, d, J 4.9 Hz), 2.81 (2H, s), 1.91-1.81 (2H, m), 1.67-1.64 (2H, m), 1.45 (9H, s), 1.13 (6H, d, J 3.6 Hz). LCMS (ES+) RT 3.35 minutes, 657 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and tert-butyl-N-(aminoethyl)carbamate (62 mg, 0.39 mmol) by the method of Example 19. The title compound was obtained as a yellow solid (106 mg, 67%). δH (CDCl3) 10.57 (1H, s), 7.49-7.45 (2H, m), 7.40-7.35 (1H, m), 6.66 (1H, br s), 6.45 (1H, br s), 4.95 (1H, br s), 3.63-3.57 (2H, m), 3.41-3.48 (2H, m), 3.07 (2H, d, J 4.9 Hz), 2.90 (2H, s), 1.41 (9H, s), 1.13 (6H, s). LCMS (ES+) RT 2.85 minutes, 617 (M+H)+.
By the method of Example 32, with (4-aminobutyl)carbamic acid tert-butyl ester (106 mg, 056 mmol). LCMS (ES+) RT 3.36 minutes, 645 (M+H)+.
Example 4 (3 g, 2 mmol) and Lawesson's reagent (1.18 g, 2.9 mmol) were dissolved in toluene (90 mL). The reaction was stirred at 110° C. for 2 h. The volatiles were removed in vacuo. The crude product was purified by chromatography (silica, 0-25% ethyl acetate in DCM) to give the title compound as a yellow solid (2.5 g, 85%). δH (DMSO-d6) 10.27 (1H, s), 8.40-8.30 (1H, m), 7.81 (1H, dd, J 10.3, 1.8 Hz), 7.68 (1H, d, J 8.4 Hz), 7.47-7.40 (1H, m), 4.31 (2H, q, J 7 Hz), 2.93 (2H, d, J 5.4 Hz), 2.84 (2H, s), 1.33 (3H, t, J 7 Hz), 0.99 (6H, s).
Intermediate 19 (400 mg, 0.75 mmol), dimethyl sulfate (0.2 mL, 2 mmol) and potassium carbonate (200 mg, 1.44 mmol) were dissolved in DCM (20 mL). The reaction was stirred at room temperature for 48 h. Water (20 mL) was added to the reaction, and the mixture was extracted with DCM (3×30 mL). The combined organic extracts were washed with aqueous saturated sodium bicarbonate solution (150 mL) and brine (150 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-20% ethyl acetate in DCM) to give the title compound as an orange solid (130 mg, 31%). δH (CDCl3) 10.76 (1H, s), 7.69 (1H, d, J 1.9 Hz), 7.53 (1H, dd, J 8.6, 1.9 Hz), 7.39 (1H, d, J 8.6 Hz), 4.34 (2H, q, J 7.0 Hz), 3.17 (2H, s), 2.67 (2H, s), 2.43 (3H, bs), 1.34 (3H, t, J 7.0 Hz), 1.03 (6H, s). LCMS (ES+) RT 2.88 minutes, 548.8 (M+H)+.
To a stirred solution of Example 4 (500 mg, 0.97 mmol) in diisopropylamine (10 mL) were added (trimethylsilyl)acetylene (95 mg, 0.97 mmol) and Pd(PPh3)2Cl2 (34 mg, 0.05 mmol), followed by CuI (18 mg, 0.10 mmol). The reaction mixture was stirred at 60° C. for 1 h, and then cooled to r.t. Water was added, and the reaction mixture was extracted with DCM (3×100 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-20% EtOAc in DCM) to give the title compound as a cream solid (263 mg, 65%). δH (DMSO-d6) 10.81 (1H, s), 8.04 (1H, t, J 4.9 Hz), 7.74-7.71 (2H, m), 7.56 (1H, dd, J 8.5, 1.9 Hz), 4.34 (2H, q, J 7.2 Hz), 4.27 (1H, s), 2.93 (2H, s), 2.86 (2H, d, J 5.1 Hz), 1.34 (3H, t, J 7.2 Hz), 0.99 (6H, s). LCMS (ES+) RT 3.77 minutes, 417 (M+H)+.
Prepared from Intermediate 21 (263 mg, 0.63 mmol) and LiOH by the method of Example 2. The title compound was obtained as a cream solid (199 mg, 65%). δH (DMSO-d6) 13.08 (1H, br s), 7.87-7.80 (1H, m), 7.67-7.62 (2H, m), 7.51 (1H, dd, J 8.5, 2.1 Hz), 4.19 (1H, s), 3.07 (2H, s), 2.85 (2H, d, J 5.1 Hz), 0.98 (6H, s). One exchangeable proton was not observed. LCMS (ES+) RT 3.24 minutes, 389 (M+H)+.
Prepared from Intermediate 22 (199 mg, 0.51 mmol) and (S)-(+)-(2,2-dimethyl-[1,3]dioxolan-4-yl)methylamine (73 mg, 0.56 mmol) by the method of Intermediate 7. The title compound was obtained as a yellow solid (98 mg, 38%). δH (CDCl3) 10.68 (1H, s), 7.65 (1H, d, J 8.5 Hz), 7.56 (1H, d, J 1.9 Hz), 7.41 (1H, dd, J 8.5, 1.9 Hz), 6.16 (1H, t, J=5.3 Hz), 6.09 (1H, t, J 5.5 Hz), 4.39-4.32 (1H, m), 4.12 (1H, dd, J 8.5, 6.6 Hz), 3.88 (1H, ddd, J 13.9, 6.6, 3.2 Hz), 3.73 (1H, dd, J 8.5, 6.0 Hz), 3.43 (1H, ddd, J 13.9, 7.3, 4.9 Hz), 3.12-3.05 (3H, m), 2.87 (2H, s), 1.47 (3H, s), 1.38 (3H, s), 1.15 (6H, d, J 2.4 Hz). LCMS (ES+) RT 3.21 minutes, 502 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (R)-2-(aminomethyl)-pyrrolidine-1-carboxylic acid tert-butyl ester (156 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (224 mg, 87%). δH (CDCl3) 10.78 (1H, br s), 7.79 (1H, br s), 7.47-7.39 (3H, m), 6.39 (1H, br s), 4.10-4.02 (1H, m), 3.73-3.69 (1H, m), 3.48-3.30 (4H, m), 3.16-3.02 (3H, m), 2.14-2.06 (1H, m), 1.99-1.81 (2H, m), 1.79-1.73 (1H, m), 1.44 (9H, s), 1.29 (3H, s), 1.27 (3H, s). LCMS (ES+) RT 3.45 minutes, 657 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (S)-2-(aminomethyl)-pyrrolidine-1-carboxylic acid tert-butyl ester (156 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (236 mg, 92%). δH (CDCl3) 10.76 (1H, dd, J 1.3, 0.7 Hz), 7.78 (1H, dd, J 1.7, 0.9 Hz), 7.46-7.38 (3H, m), 6.44 (1H, br s), 4.09-4.00 (1H, m), 3.73-3.68 (1H, m), 3.52-3.45 (2H, m), 3.43-3.30 (2H, m), 3.10-3.02 (2H, m), 2.95-2.85 (1H, m), 2.13-2.05 (1H, m), 1.98-1.81 (2H, m), 1.78-1.73 (1H, m), 1.43 (9H, s), 1.15 (3H, s), 1.10 (3H, s). LCMS (ES+) RT 3.45 minutes, 657 (M+H)+.
1,1′-Carbonyldiimidazole (222 mg, 1.37 mmol) and (S)-1-BOC-3-amino-pyrrolidine (234 mg, 1.26 mmol) was added to a solution of Example 2 (500 mg, 1.05 mmol) and the reaction mixture was stirred at RT for 18 h. Aqueous NaOH solution (5%) was added to the reaction and the mixture extracted with DCM (3×100 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-40% EtOAc in DCM) to give the title compound as a yellow solid (149 mg, 22%). δH (CDCl3) 10.06 (1H, s), 7.37-7.32 (2H, m), 7.18-7.12 (1H, m), 6.37-6.31 (2H, m), 4.56 (1H, dd, J 10.1, 4.6 Hz), 3.60 (1H, dd, J 11.5, 5.8 Hz), 3.44-3.37 (2H, m), 3.29-3.20 (1H, m), 2.91 (2H, d, J 5.1 Hz) 2.65 (2H, s), 2.19-2.10 (1H, m), 1.94-1.89 (1H, m), 1.39 (9H, s), 1.00 (6H, d, J 3.4 Hz). LCMS (ES+) RT 3.25 minutes, 641 (M+H)−.
Prepared from Intermediate 5 (996 mg, 1.56 mmol) and azetidin-3-ylcarbamic acid tert-butyl ester (536 mg, 3.11 mmol) by the method of Example 19. The title compound was obtained as a cream solid (367 mg, 37%). δH (DMSO-d6) 8.69 (1H, s), 7.92 (1H, t, J 5.0 Hz), 7.69 (1H, dd, J 10.5, 1.8 Hz), 7.56-7.51 (2H, m), 7.10 (1H, t, J 8.7 Hz), 4.12-4.05 (3H, m), 3.84-3.79 (2H, m), 2.91 (2H, d, J 5.0 Hz), 2.63 (2H, s), 1.41 (9H, s), 1.01 (6H, s). LCMS (ES+) RT 3.01 minutes, 629 (M+H)+.
Prepared from Intermediate 5 (350 mg, 0.55 mmol) and 3-aminoazetidine-1-carboxylic acid tert-butyl ester (188 mg, 1.09 mmol) by the method of Example 19. The title compound was obtained as a cream solid (212 mg, 61%). δH (DMSO-d6) 8.92 (1H, s), 8.52-8.50 (1H, m), 7.92 (1H, br s), 7.62 (1H, dd, J 10.5, 1.9 Hz), 7.46 (1H, d, J 8.4 Hz), 7.06 (1H, t, J 8.8 Hz), 4.48-4.42 (1H, m), 4.04-3.97 (2H, m), 3.63 (2H, dd, J 8.7, 5.5 Hz), 2.87 (2H, d, J 5.1 Hz), 2.68 (2H, s), 1.38 (9H, s), 0.95 (6H, s). LCMS (ES+) RT 3.30 minutes, 629 (M+H)+.
Example 1 (200 mg, 0.40 mmol) was dissolved in anhydrous THF (6.5 mL) and NaH (60% dispersion in mineral oil, 26 mg, 0.64 mmol) was added. The solution was stirred for 1 h and then cooled to 0° C. TMSCl (43 mg, 0.40 mmol) was added and, after a further 30 min, NaH (60% dispersion in mineral oil, 26 mg, 0.64 mmol) was added. After 90 min, MeI (27 μl, 0.44 mmol) was added and the reaction warmed to room temperature overnight. Brine (20 mL) was added and the aqueous layer was extracted with ether (2×25 mL). The aqueous layer was then neutralised with dilute aqueous HCl and then extracted with DCM (2×25 mL). The combined organic layers were dried (Na2SO4) and the solvent removed under reduced pressure. The orange oil was purified by column chromatography on silica gel (DCM→DCM/Et2O 10%) to yield the title compound (148 mg, 70%). δH (d6-DMSO) 10.20 (1H, s), 7.75 (1H, dd, J 10.4, 1.9 Hz), 7.62-7.59 (1H, m), 7.41 (1H, t, J 8.7 Hz), 4.28 (2H, q, J 7.0 Hz), 3.05 (2H, s), 3.00 (3H, s), 2.84 (2H, s), 1.29 (3H, t, J 7.0 Hz), 0.98 (6H, s). LCMS (ES+) RT (pH 3) 3.94 minutes, 517 (M+H)+.
Intermediate 29 (460 mg, 0.89 mmol) was dissolved in THF/H2O (3:1, 9 mL) and LiOH (106 mg, 4.43 mmol) was added. The reaction mixture was heated at reflux for 18 h and the THF was then removed under reduced pressure. The remaining aqueous solution was acidified using 10% citric acid and the precipitate was vigorously stirred for 3 h. The solid was filtered, washed with water and dried to yield the title compound as a white solid (354 mg, 81%). δH (d6-DMSO) 7.72 (1H, dd, J 10.5, 1.9 Hz), 7.65-7.59 (1H, m), 7.42 (1H, t, J 8.7 Hz), 3.26 (1H, br s), 3.07 (2H, s), 3.03 (3H, s), 2.98 (2H, s), 0.99 (6H, s). One exchangeable proton was not observed. LCMS (ES+) RT (pH 10) 1.75 minutes, 489 (M+H)+.
Intermediate 30 (354 mg, 0.72 mmol) was dissolved in DCM (10 mL) and DMF (1 mL). N-Methylmorpholine (200 mg, 1.97 mmol), HOBT (195 mg, 1.44 mmol), EDC (278 mg, 1.44 mmol) and pentafluorophenol (265 mg, 1.44 mmol) were added sequentially. The reaction mixture was stirred overnight and then brine (30 mL) was added. The aqueous layer was separated and extracted with further DCM (2×30 mL). The combined organics were dried with Na2SO4 and the solvent removed under reduced pressure. Any remaining DMF was removed by azeotroping with toluene and the resulting oil was purified using silica gel chromatography (DCM→DCM/EtOAc 10%) to yield the title compound (355 mg, 75%). δH (d6-DMSO) 9.78 (1H, s), 7.85 (1H, dd, J 10.0, 1.9 Hz), 7.72-7.67 (1H, m), 7.44 (1H, t, J 8.5 Hz), 3.14 (2H, s), 3.04 (3H, s), 2.89 (2H, s), 1.01 (6H, s). LCMS (ES+) RT (pH 3) 4.21 minutes, 655 (M+H)+.
Intermediate 31 (300 mg, 0.45 mmol) was dissolved in DCM (4.5 mL) and azetidin-3-ylmethylcarbamic acid tert-butyl ester (167 mg, 0.90 mmol) and triethylamine (125 μl, 0.90 mmol) were added. The reaction mixture was stirred for 3 h and then quenched with brine (20 mL). The aqueous layer was extracted with DCM (3×20 mL) and the combined organics were dried with Na2SO4. The solvent was then removed under reduced pressure and the crude material was purified using silica gel chromatography (DCM→DCM/EtOAc 50%) to yield the title compound (240 mg, 81%). δH (d6-DMSO) 8.71 (1H, s), 7.63 (1H, dd, J 10.5, 1.9 Hz), 7.42-7.48 (1H, m), 6.99 (1H, t, J 8.5 Hz), 3.90 (2H, m), 3.92-3.84 (2H, m), 3.58 (2H, dd, J 9.4, 5.3 Hz), 3.27 (2H, s), 3.07-3.03 (6H, m), 1.35 (9H, s), 0.98 (6H, s). One exchangeable proton was not observed. LC RT (pH 3) 2.94 minutes.
3-Oxoazetidine-1-carboxylic acid tert-butyl ester (500 mg, 2.9 mmol) was dissolved in ethanol (1.5 mL) and to this was added nitromethane (0.6 mL) and triethylamine (catalytic). The reaction mixture was stirred for 18 h and the solvent then removed under reduced pressure to yield the title compound as a white solid (650 mg, 97%). δH (d6-DMSO) 6.42 (1H, s), 4.86 (2H, s), 4.04 (2H, d, J 9.2 Hz), 3.75 (2H, d, J 9.2 Hz), 1.39 (9H, s). LCMS (ES+) RT (pH 10) 1.73 minutes, 231 (M−H)−.
Intermediate 33 (500 mg, 2.2 mmol) was dissolved in ethanol (40 mL) in a hydrogenation vessel and 10% palladium on charcoal (43 mg) was added. The vessel was charged with hydrogen to 50 psi and heated to 50° C. This was then stirred for 2 h and the catalyst was removed by filtering through a plug of celite. The solvent was removed under reduced pressure to yield a pale yellow oil. This was purified by chromatography on an amine column using DCM/MeOH 5% as the eluent to afford the title compound (306 mg, 68%). δH (d6-DMSO) 5.50 (1H, s), 3.73 (2H, d, J 8.5 Hz), 3.54 (2H, d, J 8.5 Hz), 2.60 (2H, s), 1.37 (9H, s). Some exchangeable protons were not observed.
N—BOC-ethanolamine (9.3 g, 57.7 mmol) and triethylamine (9.7 mL, 69.2 mmol) in DCM were treated with methanesulphonyl chloride (7.93 g, 69.2 mmol) and the reaction mixture stirred at r.t. for 4 h. The reaction mixture was diluted with further DCM and washed with water then dried (sodium sulphate) and concentrated in vacuo to give the title compound as a viscous oil (15 g, quant). δH (DMSO-d6) 7.03 (1H, br s), 4.16 (2H, t, J 5.6 Hz), 3.24-3.22 (2H, m), 3.15 (3H, s), 1.38 (9H, s).
Intermediate 35 (15 g, 62.8 mmol) and N-hydroxyphthalimide (11.4 g, 69 mmol) in DMF (100 mL) with triethylamine (9.7 mL, 69 mmol) were heated at 50° C. for 18 h. The DMF was removed in vacuo and the residue dissolved in toluene, washed with water, concentrated and azeotroped with heptane to remove residual DMF, then concentrated again. The residue was triturated with diethyl ether to yield the title compound. δH (DMSO-d6) 7.87 (4H, s), 6.86-6.77 (1H, m), 4.14 (2H, t, J 5.6 Hz), 3.28 (2H, q, J 5.6 Hz), 1.38 (9H, s). LCMS (ES+) RT 2.83 minutes, 329 (M+Na)+.
Intermediate 36 (1 g, 3.3 mmol) in DCM (20 mL) was treated with methyl-hydrazine (158 mg, 3.4 mmol) and the reaction mixture stirred at r.t. for 16 h. The solvent was removed, and the residue was suspended in diethyl ether, filtered and concentrated, to give the title compound. δH (DMSO-d6) 6.74-6.65 (1H, br m), 5.99-5.88 (2H, br s), 3.47 (2H, t, J 5.8 Hz), 3.09 (2H, q, J 5.8 Hz), 1.38 (9H, s). LCMS (ES−) RT 1.93 minutes, 175 (M−H)−.
Intermediate 31(370 mg, 0.57 mmol) and triethylamine (66 mg, 0.57 mmol) in DMF (5 mL) with 1-BOC-3-aminopyrrolidine (207 mg, 1.11 mmol) were stirred at r.t. for 5 days. The solvent was removed in vacuo and the residue azeotroped with heptane then partitioned between ethyl acetate and water. The organic phase was dried (sodium sulphate) and concentrated, and the residue was purified by column chromatography (SiO2; DCM/ethyl acetate) to yield the title compound. δH (DMSO-d6) 8.68 (1H, s), 8.20 (1H, d, J 5.0 Hz), 7.59 (1H, d, J 11.7 Hz), 7.44 (1H, d, J 8.6 Hz), 7.07-6.96 (1H, br m), 4.29-4.18 (1H, br m), 3.42-3.32 (1H, m), 3.29-3.21 (1H, m), 3.08 (2H, s), 3.04 (3H, s), 2.58 (2H, s), 3.04-1.92 (1H, br m), 1.79-1.68 (1H, br m), 1.39 (9H, s), 1.25 (2H, br s), 0.98 (3H, s), 0.96 (3H, s). HPLC RT 3.18 minutes.
Acetonitrile (1 mL) was added at −78° C. to a 1M solution of sodium hexamethyldisilazide (3.38 mL, 3.38 mmol) in THF (3 mL) and, after stirring for 15 minutes, (2-chloro-4-iodophenyl)isothiocyanate (500 mg, 1.69 mmol) was added. The reaction was allowed to warm to r.t. and quenched with water, the pH adjusted to pH 7 with 5% citric acid, and the reaction mixture extracted with DCM. The organic phase was separated, dried and concentrated in vacuo and the residue purified by chromatography (SiO2; 1:1-1:2 hexane:DCM) to give the title compound (350 mg, 61%). δH (DMSO-d6) 11.81 (1H, s), 7.97 (1H, d, J 1.9 Hz), 7.76 (1H, dd, J 8.3, 1.9 Hz), 7.20 (1H, d, J 8.3 Hz), 4.27 (2H, s). LCMS (ES+) RT 2.82 minutes, 376/378 (M+K)+.
From Example 5 (6.0 g, 12.2 mmol) by the method of Intermediate 5 to yield the title compound (5.17 g, 65%). δH (DMSO-d6) 10.02 (1H, s), 8.08 (1H, t, J 5.0 Hz), 8.03 (1H, d, J 1.9 Hz), 7.84 (1H, dd, J 8.4, 1.9 Hz), 7.53 (1H, d, J 8.4 Hz), 2.94 (2H, s), 2.90 (2H, d, J 5.0 Hz), 0.99 (6H, s). LCMS (ES+) RT 4.14 minutes, 657/659 (M+H)+.
To a solution of Example 2 (2.56 g, 1.5 eq) in DMF (28 mL) was added tetrafluorophenol resin (2.8 g, 1 eq) followed by the addition of 1,3-diisopropylcarbodiimide (2.54 g, 4.5 eq) and 4-(dimethylamino)pyridine (265 mg, 0.6 eq). The mixture was stirred for 24 hours at room temperature. The desired functionalised resin was then filtered and rinsed twice with sequential washes of DMF, DCM, MeOH then DCM and dried in vacuo.
Example 1 (10 g, 20 mmol) and Lawesson's reagent (4.0 g, 10 mmol) were dissolved in toluene (200 mL) and the reaction mixture stirred at 110° C. for 2 h. The reaction was concentrated in vacuo. The crude residue was triturated (1:1) with MeCN and isopropyl ether, and the precipitate filtered off and dried to give the title compound as a yellow solid (7.8 g, 75%). δH (CDCl3) 10.72 (1H, d, J 2.0 Hz), 8.06 (1H, br s), 7.46-7.36 (3H, m), 4.33 (2H, q, J 7.1 Hz), 2.97 (2H, d, J 5.8 Hz), 2.91 (2H, s), 1.35 (3H, t, J=7.1 Hz), 1.02 (6H, s). LCMS (ES+) RT 3.66 minutes, 519 (M+H)+.
Intermediate 42 (3.0 g, 5.8 mmol) and MeI (3.6 mL, 58 mmol) were dissolved in MeCN (100 mL) and stirred at r.t. for 18 h. The reaction mixture was concentrated in vacuo. The crude residue was triturated with MeCN and the precipitate was filtered off and dried to give the title compound as a yellow solid (3.3 g, 87%). δH (DMSO-d6) 11.44 (1H, br s), 10.48 (1H, br s), 7.90 (1H, dd, J 9.9, 1.5 Hz), 7.72 (1H, d, J 8.4 Hz), 7.44 (1H, t, J 8.4 Hz), 4.38 (2H, q, J 7.0 Hz), 3.25 (2H, s), 2.95 (2H, s), 2.79 (3H, s), 1.35 (3H, t, J=7.1 Hz), 1.12 (6H, s). LCMS (ES+) RT 2.94 minutes, 533 (M+H)+.
To a stirred solution of 5,5-dimethylcyclohexane-1,3-dione (98.0 g, 699.1 mmol) and malononitrile (46.2 g, 699.1 mmol) in EtOH (400 mL) at r.t. was added piperidine (10 mL, 99.9 mmol) dropwise over 15 minutes. The reaction mixture was heated to reflux for 3 days, and then concentrated in vacuo. The residue was dissolved in EtOAc (500 mL), and the solution was dried (MgSO4), filtered and concentrated in vacuo. Purification by column chromatography (SiO2, 15% MeOH/DCM) gave the title compound (108.2 g, 82%) as a yellow solid. δH (DMSO-d6) 8.31 (2H, br s), 2.51 (2H, t, J=1.8 Hz), 2.30 (2H, s), 1.04 (6H, s).
To a stirred solution of Intermediate 44 (50.8 g, 269.9 mmol) and sulphur (10.3 g, 323.9 mmol) in EtOH (600 mL) at r.t. was added morpholine (47.0 mL, 539.8 mmol) dropwise. The reaction mixture was heated to 80° C. for 24 h, and then cooled. The precipitate formed was filtered and washed with cold Et2O to give the title compound (41.2 g, 53%) as a brown solid that was used without further purification. δH (DMSO-d6) 8.31 (2H, br s), 2.51 (2H, t, J 1.8 Hz), 2.30 (2H, s), 1.04 (6H, s). LCMS (ES+) 221.0 (M+H)+, RT 2.67 minutes.
To a stirred solution of CuBr2 (4.2 g, 19.1 mmol) in MeCN (100 mL) at 0° C. was added tert-butyl nitrite (2 mL, 15.0 mmol) dropwise. The reaction mixture was then stirred at this temperature for 10 minutes before Intermediate 45 (3.0 g, 13.6 mmol) was added portionwise. The reaction mixture was then allowed to warm to r.t., stirred for 4 h, and then partitioned between 2M aqueous HCl (200 mL) and EtOAc (3×200 mL). The combined organic fractions were dried (MgSO4), filtered and concentrated in vacuo. Purification by column chromatography (SiO2, 25% DCM/hexanes) gave the title compound (1.5 g, 40%) as an off-white solid. δH (DMSO-d6) 2.84 (2H, s), 2.51 (2H, s), 1.07 (6H, s).
Intermediate 46 (100 mg, 0.35 mmol) and dimethyl disulfide (63 μL, 0.70 mmol) were stirred in THF (3.5 ml) and the mixture cooled to −78° C. tert-Butyllithium (1.7M in pentane, 0.42 ml) was added dropwise and the reaction was stirred for 2 h under an inert atmosphere. A saturated solution of ammonium chloride (10 mL) was added and the reaction mixture was allowed to warm to room temperature. The reaction mixture was extracted with ether (3×15 ml) and the organics combined and dried with sodium sulfate. The solvent was removed under reduced pressure and the crude product was purified using silica gel chromatography (DCM/hexane 1:1→DCM gradient elution) to yield the title compound (49 mg, 44%) contaminated with a 20% impurity. δH (d6-DMSO) 2.79 (5H, m), 2.46 (2H, s), 1.06 (6H, s). LCMS (ES+) RT (pH 3) 2.73 minutes, 252 (M+H)+.
Intermediate 47 (100 mg, 0.38 mmol) was dissolved in DCM (4 mL) and the solution cooled to 0° C. mCPBA was added and the reaction was allowed to warm to r.t. After 4 h, the product was washed with a saturated solution of NaHCO3 (10 mL). The DCM layer was dried with sodium sulfate. The solvent was removed to yield an oil which was triturated with ether/hexane to yield the title compound as a white solid (36 mg, 35%). δH (d6-DMSO) 3.58 (3H, s), 2.84 (2H, s), 2.61 (2H, s), 1.09 (6H, s). LC RT (pH 10) 2.24 minutes.
To a stirred solution of ethyl 3,3-dimethylacrylate (5.0 g, 39.1 mmol) in EtOH (20 mL) in a Parr® reactor at 0° C. was added liquid NH3 (approximately 20 mL). The reactor was sealed and heated to 90° C. for 24 h. The reaction mixture was then cooled to r.t., bubbled with nitrogen to remove the residual NH3 and treated with 4M HCl in 1,4-dioxane (10 mL). The reaction mixture was stirred for 30 minutes at r.t. and then evaporated in vacuo to dryness. The resulting grey paste was triturated with DCM, filtered and dried to give the title compound (5.0 g, 70%) as a grey solid that was used without further purification. δH (CDCl3) 8.27 (3H, br. s), 4.10 (2H, q, J 7.1 Hz), 2.65 (2H, s), 1.26 (6H, s), 1.20 (3H, t, J 7.1 Hz).
To a stirred suspension of Intermediate 49 (5.0 g, 27.4 mmol) in DCM (40 mL) was added triethylamine (11.1 g, 15.3 mL, 109.6 mmol). The reaction mixture was then cooled to 0° C. and ethyl malonyl chloride (4.4 g, 3.7 mL, 28.8 mmol) was added dropwise. The suspension was stirred at r.t. for 2 h before it was diluted with DCM (50 mL) and washed with aqueous 1M HCl (50 mL) and water (2×50 mL). The organics were dried over MgSO4, filtered and concentrated in vacuo to give the title compound (5.0 g, 71%) as an orange oil that was used without further purification. δH (DMSO-d6) 7.75 (1H, br s), 4.15-3.95 (4H, m), 3.14 (2H, s), 2.71 (2H, s), 1.29 (6H, s), 1.21-1.11 (6H, m).
To a stirred solution of NaOEt, prepared in situ from Na (0.53 g, 23.16 mmol) in EtOH (30 mL), was added dropwise a solution of Intermediate 50 (5.00 g, 19.30 mmol) in toluene (30 mL) and the reaction mixture was heated to 80° C. for 2 h. The solution was then concentrated to approximately 10 mL and the residue was dissolved in toluene (30 mL) and extracted with water (3×30 mL). The combined aqueous layers were acidified to pH 2-3 with aqueous 1M HCl and extracted with EtOAc (4×50 mL). The combined organic fractions were dried (MgSO4), filtered and evaporated in vacuo to give a pale yellow solid that was dissolved in MeCN (90 mL) containing 1% water. The solution was heated to reflux for 2 h and then evaporated in vacuo to dryness. The resulting solid was triturated with isopropyl ether, filtered and dried to give the title compound (1.55 g, 57%) as a cream solid that was used without further purification. Both the keto and enol forms were observed (ratio 3.6:1 keto/enol). δH (DMSO-d6) 10.29 (1H, br s, enol), 8.14 (1H, br s, keto), 6.66 (1H, s, enol), 4.81 (1H, s, enol), 3.15 (2H, s), 2.51 (2H, s), 1.20 (6H, s, keto), 1.18 (6H, s, enol).
To a stirred solution of sulphur (1.0 g, 31.0 mmol), Intermediate 51 (4.0 g, 28.0 mmol) and ethyl cyanoacetate (3.7 g, 3.5 mL, 29.0 mmol) in EtOH (20 mL) at 45° C. was added morpholine (2.9 g, 2.9 mL, 33.0 mmol) dropwise over 15 minutes. The reaction mixture was stirred at this temperature for 15 minutes and then at 65° C. for 48 h before it was cooled and concentrated in vacuo. To the residue was added water and the resulting solid was filtered and washed with water to give the title compound as a pale brown solid (4.1 g, 54%). δH (DMSO-d6) 7.86 (2H, s), 7.28 (1H, s), 4.21 (2H, q, J 7.0 Hz), 2.88 (2H, s), 1.27 (3H, t, J 7.1 Hz), 1.23 (6H, s). LCMS (ES+) 269.1 (M+H)+.
To a stirred suspension of Intermediate 53 (0.20 g, 0.75 mmol) in MeCN (5 mL) at 0-5° C. was added CuBr2 (0.20 g, 0.90 mmol) followed by tert-butyl nitrite (0.10 g, 0.10 mL, 0.80 mmol) dropwise. The reaction mixture was stirred at this temperature for 10 minutes before it was partitioned between EtOAc (50 mL) and water (50 mL). The organics were separated, washed with water (3×20 mL) and brine (20 mL), dried (Na2SO4), filtered and concentrated in vacuo. The crude residue was washed with Et2O to give the title compound as a pale brown solid (0.15 g, 61%). δH (DMSO-d6) 8.53 (1H, s), 4.32 (2H, q, J 7.0 Hz), 3.10 (2H, s), 1.33 (3H, t, J 7.1 Hz), 1.26 (6H, s). LCMS (ES+) 332.0 and 334.0 (M+H)+.
To a stirred solution of Intermediate 53 (1.0 g, 3.0 mmol) in anhydrous THF (10 mL) cooled to 0° C. was added portionwise sodium hydride (144 mg, 3.6 mmol, 60% dispersion in mineral oil) and the resulting mixture allowed to stir for 30 minutes. Di-tert-butyl dicarbonate (654 mg, 3.0 mmol) was added and the reaction mixture allowed to warm to ambient temperature whereupon anhydrous DMF (5 mL) was added to aid solubility. The resultant solution was stirred for 18 hours. The reaction mixture was poured into saturated brine (100 mL) and extracted with EtOAc (3×100 mL). The combined organic fractions were dried (Na2SO4), filtered and the volatiles removed in vacuo. Purification by column chromatography (SiO2, 90-100% DCM in isohexanes) gave the title compound as a yellow oil (602 mg, 58%). δH (DMSO-d6) 4.38 (2H, q, J 7.1 Hz), 3.22 (2H, s), 1.55 (9H, s), 1.49 (6H, s), 1.39 (3H, t, J 7.1 Hz). LCMS (ES+) RT 3.46 minutes, 434 (M+H)+.
To a stirred solution of Intermediate 54 (602 mg, 1.4 mmol) and dimethyl disulphide (0.15 mL, 1.7 mmol) in anhydrous THF (10 mL) cooled to −78° C. was added dropwise tert-butyllithium (1.6 mL, 2.78 mmol, 1.7M in pentane). The resultant reaction mixture was stirred at −78° C. for two hours. The reaction was quenched by addition of 10% aqueous ammonium chloride solution (50 mL) and the aqueous phase extracted with DCM (3×100 mL). The combined organic fractions were dried (Na2SO4), filtered and the volatiles removed in vacuo. Purification by column chromatography (SiO2, 0-60% EtOAc in isohexanes) gave the title compound as a yellow oil (366 mg, 65%). δH (DMSO-d6) 4.28 (2H, q, J 7.1 Hz), 3.18 (2H, s), 2.65 (3H, s), 1.48 (9H, s), 1.42 (6H, s), 1.29 (3H, t, J 7.1 Hz). LCMS (ES+) RT 3.36 minutes, 400 (M+H)+.
To a stirred solution of Intermediate 55 (366 mg, 0.92 mmol) in DCM (5 mL) was added 3-chloroperoxybenzoic acid (206 mg, 1.2 mmol). The resultant reaction mixture was stirred at ambient temperature for 18 hours. The reaction was quenched by addition of saturated aqueous NaHCO3 solution (50 mL) and the aqueous phase extracted with DCM (3×100 mL). The combined organic fractions were dried (Na2SO4), filtered and the volatiles removed in vacuo to give a 1:1 mixture of the title compound and the sulphone as a clear oil (258 mg). The material was used in the next step without further purification. LCMS (ES+) sulphoxide RT 2.70 minutes, 416 (M+H)+; sulphone RT 2.94 minutes, 432 (M+H)+.
Intermediate 2 (11.2 g, 47.6 mmol) was added to a mixture of Intermediate 4 (17.5 g, 47.6 mmol) and caesium carbonate (15.5 g, 47.6 mmol) in DMF (400 mL) and the mixture heated at 80° C. for 18 h. The reaction was cooled and poured onto iced water (200 mL) and 2M HCl added (30 mL). The precipitate was filtered and washed with MeCN to give the title compound as a yellow solid (11.53 g, 48%). δH (DMSO-d6) 10.32 (1H, s), 7.98 (1H, t, J 4.8 Hz), 7.76 (1H, dd, J 10.4, 1.6 Hz), 7.63 (1H, d, J 8.6 Hz), 7.43 (1H, t, J 8.6 Hz), 4.30 (2H, q, J 7.1 Hz), 2.89 (2H, s), 2.84 (2H, d, J 5.0 Hz), 1.32 (3H, t, J=7.1 Hz), 0.98 (6H, s). LCMS (ES+) RT 3.64 minutes, 503 (M+H)+.
Lithium hydroxide monohydrate (1.43 g, 59.8 mmol) was added to a solution of Example 1 (10 g, 19.92 mmol) in THF (20 mL) and water (5 mL) and heated to reflux for 18 h. The reaction mixture was concentrated in vacuo and 5% citric acid (10 mL) added. The resulting precipitate was filtered and dried in vacuo to give the title compound as a yellow solid (7.3 g, 77%). δH (DMSO, d6) 11.65 (1H, br s), 7.87 (1H, t, J 4.8 Hz), 7.72 (1H, dd, J 10.6, 1.8 Hz), 7.61 (1H, d, J 8.5 Hz), 7.43 (1H, t, J 8.7 Hz), 3.00 (2H, s), 2.84 (2H, d, J 4.9 Hz), 0.97 (6H, s). LCMS (ES+) RT 2.98 minutes, 473 (M+H)+.
Caesium carbonate (142 mg, 0.74 mmol) and 3,4-difluoronitrobenzene (118 mg, 0.74 mmol) were added to a solution of Intermediate 6 (100 mg, 0.34 mmol) in DMF (5 mL) and stirred at r.t. for 18 h. Brine (10 mL) was added to the reaction, and the mixture extracted with EtOAc (3×10 mL). The combined organic extracts were dried (MgSO4) and concentrated in vacuo. The crude product was triturated with MeOH (5 mL) to give the title compound as a yellow solid (45 mg, 31%). δH (DMSO-d6) 10.86 (1H, s), 8.25-8.15 (3H, m), 7.72 (1H, t, J 8.5 Hz), 4.30 (2H, q, J 7.0 Hz), 2.91 (2H, s), 2.87 (2H, d, J 4.9 Hz), 1.29 (3H, t, J 7.0 Hz), 0.99 (6H, s). LCMS (ES+) RT 3.05 minutes, 422 (M+H)+.
Caesium carbonate (2.77 g, 8.51 mmol) and 2-chloro-4-iodo-1-fluorobenzene (2.18 g, 8.51 mmol) were added to a solution of Intermediate 6 (2.0 g, 7.09 mmol) in DMF (20 mL) and heated at 65° C. for 18 h. Brine (100 mL) was added to the reaction and the mixture extracted with DCM (3×50 mL). The combined organic extracts were dried (MgSO4) and concentrated in vacuo. The residual DMF was azeotroped with heptane. The crude product was purified by chromatography (silica, 0-30% EtOAc in DCM) to give the title compound as a cream solid (916 mg, 25%). δH (DMSO-d6) 10.60 (1H, s), 8.01 (1H, t, J 5.0 Hz), 7.95 (1H, d, J 2.0 Hz), 7.78 (1H, dd, J 8.6, 2.0 Hz), 7.54 (1H, d, J 8.6 Hz), 4.32 (2H, q, J 7.1 Hz), 2.91 (2H, s), 2.85 (2H, d, J 5.2 Hz), 1.33 (3H, t, J 7.1 Hz), 0.99 (6H, s). LCMS (ES+) RT 3.81 minutes, 519 (M+H)+.
Lithium hydroxide monohydrate (49 mg, 1.16 mmol) was added to a solution of Example 4 (300 mg, 0.58 mmol) in THF (20 mL) and water (5 mL) and heated to reflux for 18 h. The reaction mixture was concentrated in vacuo and 5% citric acid (10 mL) added. The resulting precipitate was filtered and dried in vacuo to give the title compound as a cream solid (225 mg, 79%). δH (DMSO-d6) 13.90 (1H, br s), 7.79 (1H, d, J 2.0 Hz), 7.75-7.70 (1H, m), 7.67 (1H, dd, J 8.6, 2.0 Hz), 7.43 (1H, d, J 8.6 Hz), 3.13 (2H, s), 2.82 (2H, d, J 5.0 Hz), 0.96 (6H, s). LCMS (ES+) RT 3.10 minutes, 491 (M+H)+.
A mixture of Intermediate 7 (130 mg, 0.21 mmol) in MeOH (20 mL) and 2M HCl (2 mL) was stirred at r.t. for 1 h. The reaction was concentrated in vacuo to give the title compound as a cream solid (90 mg, 75%). δH (DMSO-d6) 9.42 (1H, s), 7.96 (1H, t, J 4.6 Hz), 7.82-7.77 (2H, m), 7.64 (1H, dd, J 8.6, 1.9 Hz), 7.22 (1H, d, J 8.6 Hz), 4.76 (1H, d, J 4.9 Hz), 4.54 (1H, t, J 5.5 Hz), 3.58-3.54 (1H, m), 3.39-3.29 (3H, m), 3.28-3.10 (1H, m), 2.88 (2H, d, J 4.9 Hz), 2.77 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.64 minutes, 562 (M+H)+.
A mixture of Intermediate 8 (100 mg, 0.15 mmol) and 4M HCl in 1,4-dioxane (5 mL) was stirred at ambient temperature for 1 hour. The resulting precipitate was filtered and dried in vacuo to give the title compound as a cream solid (80 mg, 95%). δH (DMSO-d6) 9.35 (1H, br s), 9.26 (1H, br s), 8.48 (1H, s), 7.90 (1H, t, J 4.9 Hz), 7.82 (1H, d, J 1.9 Hz), 7.59 (1H, dd, J 8.5, 1.9 Hz), 6.97 (1H, d, J 8.5 Hz), 3.61-3.57 (6H, m), 3.12-3.04 (4H, m), 2.87 (2H, d, J 4.8 Hz), 0.96 (6H, s). LCMS (ES+) RT 2.19 minutes, 559 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and methylamine hydrochloride (36 mg, 0.54 mmol) by the method of Intermediate 7. The title compound was obtained as a cream solid (83 mg, 32%). δH (DMSO-d6) 9.45 (1H, s), 7.95-7.93 (1H, br m), 7.84-7.81 (2H, br m), 7.64 (1H, dd, J 8.6, 2.0 Hz), 7.22 (1H, d, J 8.6 Hz), 2.90 (2H, d, J 5.0 Hz), 2.75 (3H, s), 2.72 (2H, d, J 4.6 Hz), 0.97 (6H, s). LCMS (ES+) RT 3.02 minutes, 504 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and pyrrolidine (38 mg, 0.54 mmol) by the method of Intermediate 7. The title compound was obtained as a pink solid (91 mg, 33%). δH (DMSO-d6) 8.37 (1H, s), 7.92-7.88 (1H, br m), 7.75 (1H, d, J 2.0 Hz), 7.53 (1H, dd, J 8.5, 2.0 Hz), 6.87 (1H, d, J 8.5 Hz), 3.40-3.10 (6H, br m), 2.87 (2H, d, J 4.7 Hz), 1.80-1.65 (4H, br m), 0.94 (6H, s). LCMS (ES+) RT 3.02 minutes, 544 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and 1-methylpiperazine (54 mg, 0.54 mmol) by the method of Intermediate 7. The title compound was obtained as an off-white solid (113 mg, 39%). δH (CDCl3) 7.61 (1H, d, J 2.0 Hz), 7.44 (1H, dd, J 8.6, 2.0 Hz), 7.37 (1H, br s), 7.25 (1H, d, J 8.6 Hz), 6.05-5.95 (1H, br m), 3.65-3.40 (4H, br m), 2.96 (2H, d, J 5.3 Hz), 2.53 (2H, s), 2.45-2.44 (2H, br m), 2.42-2.25 (5H, br m), 0.99 (6H, s). LCMS (ES+) RT 2.41 minutes, 573 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and N,O-dimethylhydroxylamine hydrochloride (53 mg, 0.54 mmol) by the method of Intermediate 7. The title compound was obtained as a pale yellow solid (60 mg, 16%). δH (DMSO-d6) 8.22 (1H, s), 7.96-7.93 (1H, br m), 7.77 (1H, d, J 2.0 Hz), 7.57 (1H, dd, J 8.6, 2.0 Hz), 6.99 (1H, d J 8.6 Hz), 3.46 (3H, s), 3.16 (3H, s), 2.89 (2H, d, J 5.1 Hz), 2.53 (2H, s), 0.96 (6H, s). LCMS (ES+) RT 3.23 minutes, 534 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and 3-(2-aminoethyl)pyridine (93 mg, 0.76 mmol) by the method of Intermediate 7. The title compound was obtained as a pale green solid (165 mg, 54%). δH (DMSO-d6) 9.26 (1H, s), 8.44 (1H, d, J 2.8 Hz), 8.39 (1H, dd, J 4.7, 1.6 Hz), 8.00-7.92 (2H, m), 7.65 (1H, d, J 2.0 Hz), 7.64 (2H, dd, J 8.5, 2.0 Hz), 7.27 (1H, dd, J 7.1, 4.7 Hz), 7.20 (1H, d, J 8.5 Hz), 3.54-3.52 (2H, m), 2.86-2.78 (4H, m), 2.56 (2H, s), 0.89 (6H, s). LCMS (ES+) RT 3.00 minutes, 595 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and 4-(2-aminoethyl)pyridine (93 mg, 0.76 mmol) by the method of Intermediate 7. The title compound was obtained as an off-white solid (86 mg, 28%). δH (DMSO-d6) 9.23 (1H, s), 8.43 (2H, dd, J 4.5, 1.5 Hz), 8.05-7.90 (2H, br m), 7.82 (1H, d, J 2.0 Hz), 7.64 (1H, dd, J 8.6, 2.0 Hz), 7.24 (2H, dd, J 4.5, 1.5 Hz), 7.18 (1H, d, J 8.6 Hz), 3.55-3.49 (2H, m), 2.86-2.78 (4H, m), 2.55 (2H, s), 0.87 (6H, s). LCMS (ES+) RT 2.87 minutes, 595 (M+H)+.
Prepared from Example 5 (250 mg, 0.51 mmol) and 1,2-diamino-2-methylpropane (67 mg, 0.76 mmol) by the method of Intermediate 7. The title compound was obtained as an off-white solid (27 mg, 9%). δH (DMSO-d6) 8.98 (1H, br s), 8.35 (1H, br s), 7.85 (1H, br m), 7.77 (1H, d, J 2.0 Hz), 7.61 (1H, dd, J 8.6, 2.0 Hz), 7.23 (1H, d, J 8.6 Hz), 3.37-3.33 (4H, br m), 2.91 (2H, s), 2.88 (2H, d, J 4.9 Hz), 1.19 (6H, s), 0.98 (6H, s). LCMS (ES+) RT 2.24 minutes, 561 (M+H)+.
Prepared from Intermediate 9 (74 mg, 0.12 mmol) by the method of Example 6.
The title compound was obtained as an off-white solid (25 mg, 36%). δH (DMSO-d6) 7.90-7.85 (1H, br m), 7.78 (1H, d, J 1.9 Hz), 7.60 (1H, dd, J 8.6, 1.9 Hz), 7.16 (1H, d, J=8.6 Hz), 3.86-3.81 (1H, m), 3.72-3.67 (2H, m), 3.36 (2H, d, J 4.4 Hz), 2.86 (2H, d, J 5.1 Hz), 2.76-2.72 (2H, br m), 0.97 (6H, s). LCMS (ES+) RT 2.66 minutes, 580 (M+H)+.
Prepared from Intermediate 11(74 mg, 0.12 mmol) by the method of Example 6. The title compound was obtained as an off-white solid (51 mg, 63%). δH (DMSO-d6) 9.32 (1H, s), 7.97-7.90 (2H, br m), 7.81 (1H, d, J 2.0 Hz), 7.63 (1H, dd, J 8.6, 2.0 Hz), 7.18 (1H, d, J 8.6 Hz), 4.53-4.46 (2H, m), 3.47-3.43 (1H, m), 3.27-3.20 (2H, m), 2.89 (2H, d, J 4.9 Hz), 2.75 (2H, s), 2.54 (2H, s), 1.65-1.61 (1H, br m), 1.43-1.38 (1H, m), 0.97 (6H, s). LCMS (ES+) RT 2.51 minutes, 578 (M+H)+.
Prepared from Intermediate 12 (115 mg, 0.17 mmol) by the method of Example 7. The title compound was obtained as a yellow powder (106 mg, 100%). δH (DMSO-d6) 9.03 (1H, s), 8.81-8.80 (1H, br m), 8.60-8.50 (1H, br m), 8.18 (1H, t, J 5.9 Hz), 8.07 (1H, t, J 5.0 Hz), 7.84 (1H, d, J 2.0 Hz), 7.66 (1H, dd, J 8.6, 2.0 Hz), 7.11 (1H, d, J 8.6 Hz), 3.29-3.23 (2H, br m), 3.14 (2H, t, J 5.9 Hz), 2.95 (2H, d, J 5.0 Hz), 2.87-2.78 (4H, br m), 1.79-1.74 (3H, br m), 1.43-1.30 (1H, m), 1.03 (6H, s). LCMS (ES+) RT 2.20 minutes, 587 (M+H)+.
To a solution of Intermediate 14 (123 mg, 0.24 mmol) in DMF (5 mL) was added 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide (47 mg, 0.24 mmol), 1-hydroxy-benzotriazole (33 mg, 0.24 mmol), 4-methylmorpholine (50 mg, 0.48 mmol) and (S)-(+)-(2,2-dimethyl-[1,3]-dioxolan-4-yl)methylamine (36 mg, 0.27 mmol). The mixture was stirred at room temperature for 7 days. 2M HCl (2 mL) was added to the reaction and the mixture stirred for 3 hours. The reaction was poured into brine (50 mL), and extracted with ethyl acetate (3×50 mL). The combined organics were dried (Na2SO4), filtered and the solvents removed in vacuo. The crude residue was purified by column chromatography (SiO2, 20-100% ethyl acetate in dichloromethane) to give the title compound as an off-white solid (62 mg, 44%). δH (DMSO-d6) 9.26 (1H, s), 7.81-7.80 (2H, m), 7.63 (1H, dd, J2.0, 8.6 Hz), 7.17 (1H, d, J 8.6 Hz), 4.76 (1H, d, J 5.0 Hz), 4.54 (1H, t, J 5.7 Hz), 3.58-3.51 (1H, m), 3.35-3.25 (3H, m), 3.17-3.10 (3H, m), 3.05 (3H, s), 2.70 (2H, s), 0.99 (6H, s). LCMS (ES+) RT 2.77 minutes, 578 (M+H)+.
1-Amino-2-methylpropan-2-ol (35 mg, 0.39 mmol) was added to a mixture of Intermediate 5 (250 mg, 0.39 mmol) and triethylamine (54 μL, 0.39 mmol) in DCM (5 mL) and stirred at r.t. for 18 h. H2O (50 mL) was added to the reaction, and the mixture was extracted with DCM (3×10 mL). The combined organic extracts were dried (MgSO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-40% EtOAc in DCM) to give the title compound as a cream solid (102 mg, 48%). δH (DMSO-d6) 9.12 (1H, s), 7.94 (1H, br s), 7.72 (1H, br s), 7.62 (1H, dd, J 10.7, 1.8 Hz), 7.49 (1H, d, J 8.6 Hz), 7.12 (1H, t, J 8.7 Hz), 4.49 (1H, s), 3.15 (2H, d, J 5.9 Hz), 2.88 (2H, d, J 5.0 Hz), 2.75 (2H, s), 1.06 (6H, s), 0.96 (6H, s). LCMS (ES+) RT 3.24 minutes, 546 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 2-methylpropane-1,2-diamine (172 mg, 1.95 mmol) by the method of Example 19. The title compound was obtained as a cream solid (45 mg, 21%). δH (DMSO-d6) 9.80 (1H, br s), 7.58-7.52 (1H, m), 7.48 (1H, dd, J 10.8, 1.8 Hz), 7.41 (1H, d, J 8.6 Hz), 7.19 (1H, t, J 8.8 Hz), 5.75 (3H, br s), 3.21 (2H, s), 2.96 (2H, s), 2.81 (2H, d, J 4.8 Hz), 1.09 (6H, s), 0.96 (6H, s). LCMS (ES+) RT 2.39 minutes, 545 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 2-amino-2-methylpropan-1-ol (35 mg, 0.39 mmol) by the method of Example 19. The title compound was obtained as a cream solid (62 mg, 29%). δH (DMSO-d6) 8.78 (1H, d, J 1.4 Hz), 7.92 (1H, t, J 5.0 Hz), 7.61 (1H, dd, J 10.7, 1.9 Hz), 7.46 (1H, d, J 8.5 Hz), 7.30 (1H, s), 7.05 (1H, t, J 8.8 Hz), 4.88 (1H, t, J 5.6 Hz), 3.37 (2H, d, J 5.6 Hz), 2.85 (2H, d, J 5.1 Hz), 2.68 (2H, s), 1.15 (6H, s), 0.96 (6H, s). LCMS (ES+) RT 3.28 minutes, 546 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and propane-1,3-diamine (145 mg, 1.95 mmol) by the method of Example 19. The title compound was obtained as a cream solid (120 mg, 58%). δH (DMSO-d6) 8.36 (4H, br s), 7.86 (1H, t, J 4.9 Hz), 7.59 (1H, dd, J 10.7, 1.9 Hz), 7.46 (1H, d, J 8.6 Hz), 7.13 (1H, t, J 8.7 Hz), 3.24 (2H, t, J 6.6 Hz), 2.85 (2H, d, J 4.9 Hz), 2.80-2.75 (4H, m), 1.77-1.68 (2H, m), 0.95 (6H, s). LCMS (ES+) RT 2.37 minutes, 531 (M+H)+.
4M HCl in 1,4-dioxane (2 mL) was added to a mixture of Intermediate 15 (130 mg, 0.20 mmol) and 1,4-dioxane (5 mL) in DCM (5 mL) and stirred at r.t. for 1 h. The reaction was concentrated in vacuo to give the title compound as a yellow solid (84 mg, 71%). δH (DMSO-d6) 9.04 (1H, br s), 8.86 (2H, br s), 8.20 (1H, d, J 7.4 Hz), 7.95 (1H, t, J 4.9 Hz), 7.61 (1H, dd, J 10.7, 1.8 Hz), 7.45 (1H, d, J 8.6 Hz), 7.05 (1H, t, J 8.8 Hz), 4.02 (1H, br s), 3.18-3.10 (2H, m), 2.86 (2H, d, J 4.9 Hz), 2.81-2.68 (4H, m), 1.77-1.62 (3H, m), 1.47-1.23 (1H, m), 0.96 (6H, d, J 1.0 Hz). LCMS (ES+) RT 2.39 minutes, 557 (M+H)+.
Prepared from Intermediate 16 (172 mg, 0.26 mmol) and 4M HCl in 1,4-dioxane (2 mL) by the method of Example 23. The title compound was obtained as a yellow solid (120 mg, 78%). δH (DMSO-d6) 9.16 (1H, br s), 8.97 (1H, br s), 8.87 (1H, s), 8.24 (1H, d, J 7.8 Hz), 7.95 (1H, t, J 5.0 Hz), 7.61 (1H, dd, J 10.7, 1.9 Hz), 7.45 (1H, d, J 8.5 Hz), 7.05 (1H, t, J 8.7 Hz), 4.04 (1H, br s), 3.18-3.10 (2H, m), 2.86 (2H, d, J 5.0 Hz), 2.81-2.75 (2H, m), 2.69 (2H, d, J 3.6 Hz), 1.84-1.59 (3H, m), 1.48-1.37 (1H, m), 0.96 (6H, d, J 0.9 Hz). LCMS (ES+) RT 2.39 minutes, 557 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 1,3-diamino-2-propanol (450 mg, 1.95 mmol) by the method of Example 19. The title compound was obtained as a yellow solid (45 mg, 21%). δH (MeOD-d4) 7.56-7.48 (2H, m), 7.22 (1H, t, J 8.7 Hz), 3.78-3.71 (1H, m), 3.59-3.31 (2H, m), 3.03 (2H, s), 2.84 (2H, s), 2.77 (1H, dd, J 13.2, 4.3 Hz), 2.65 (1H, dd, 13.2, 7.3 Hz), 1.08 (6H, s). LCMS (ES+) RT 2.36 minutes, 547 (M+H)+.
Prepared from Intermediate 17 (106 mg, 0.17 mmol) and 4M HCl in 1,4-dioxane (2 mL) by the method of Example 23. The title compound was obtained as a yellow solid (52 mg, 55%). δH (DMSO-d6) 9.35 (1H, s), 8.16 (1H, br s), 8.05 (3H, m), 7.93 (1H, t, J 4.8 Hz), 7.64 (1H, dd, J 10.6, 1.8 Hz), 7.50 (1H, d, J 8.5 Hz), 7.19 (1H, t, J 8.7 Hz), 3.46-3.39 (2H, m), 2.92-2.87 (4H, m), 2.78 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.06 minutes, 517 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 1-amino-2-propanol (59 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (30 mg, 14%). δH (DMSO-d6) 9.09 (1H, s), 7.92 (1H, br s), 7.81 (1H, br s), 7.63 (1H, dd, J 10.6, 1.9 Hz), 7.48 (1H, d, J 8.5 Hz), 7.13 (1H, t, J 8.7 Hz), 4.69 (1H, d, J 4.7 Hz), 3.69-3.63 (1H, m), 3.12 (2H, t, J 5.9 Hz), 2.87 (2H, d, J 5.0 Hz), 2.74 (2H, s), 1.02 (3H, d, J 6.2 Hz), 0.96 (6H, s). LCMS (ES+) RT 3.12 minutes, 532 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 3-hydroxyazetidine hydrochloride (86 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (62 mg, 30%). δH (DMSO-d6) 8.69 (1H, s), 7.87 (1H, br s), 7.63 (1H, d, J 10.6 Hz), 7.55 (1H, d, J 8.0 Hz), 7.01 (1H, t, J 8.6 Hz), 5.66 (1H, d, J 5.6 Hz), 4.47-4.39 (1H, m), 4.09-4.03 (2H, m), 3.66-3.64 (2H, m), 2.85 (2H, d, J 4.4 Hz), 2.56 (2H, s), 0.96 (6H, s). LCMS (ES+) RT 2.52 minutes, 530 (M+H)+.
Intermediate 6 (0.6 g, 2.13 mmol) in DMF (5 mL) was treated with 3,4-difluoro-benzaldehyde (0.3 g, 2.13 mmol) and Cs2CO3 (0.7 g, 2.13 mmol), then the reaction was heated at 80° C. for 18 h. After pouring onto water the solution was extracted into DCM and then toluene. The combined organic phases were dried over Na2SO4 and concentrated in vacuo. The crude residue was purified by chromatography (SiO2, DCM-ethyl acetate) to give the title compound (280 mg). δH (DMSO-d6) 10.86 (1H, d, J 3.2 Hz), 9.89 (1H, d, J 1.9 Hz), 8.11 (1H, t, J 5.0 Hz), 7.95-7.80 (3H, m), 4.33 (2H, q, J 7.1 Hz), 2.93 (2H, s), 2.87 (2H, d, J 5.1 Hz), 1.32 (3H, t, J 7.1 Hz), 1.00 (6H, s). LCMS (ES+) RT 3.04 minutes, 403 (M)−.
Example 29 (265 mg, 0.66 mmol) in ethanol (5 mL) was treated with sodium borohydride (25 mg, 0.72 mmol) and stirred at r.t. for 30 minutes. The reaction was quenched with water, the ethanol removed in vacuo and the residue extracted into DCM. After drying over sodium sulphate and evaporation in vacuo the product was chromatographed (SiO2, DCM-ethyl acetate) to yield the title compound (210 mg). δH (DMSO-d6) 10.15 (1H, s), 7.94 (1H, t, J 4.8 Hz), 7.58 (1H, t, J 8.4 Hz), 7.29 (1H, d, J 11.9 Hz), 7.24 (1H, d, J 8.5 Hz), 5.31 (1H, t, J 5.8 Hz), 4.51 (2H, d, J 5.7 Hz), 4.32 (2H, q, J 7.1 Hz), 2.91 (2H, s), 2.84 (2H, d, J 5.1 Hz), 1.34 (3H, t, J 7.1 Hz), 1.00 (6H, s). LCMS (ES+) RT 2.88 minutes, 407 (M+H)+.
Intermediate 5 (200 mg, 0.31 mmol) and 1M hydrazine in THF (0.63 mL, 0.63 mmol) were stirred at r.t. for 18 h. The reaction mixture was partitioned between DCM and 2M NaOH solution, causing a white precipitate to fall out. The precipitate was filtered off, washed with water and dried in vacuo, yielding the title compound. δH (DMSO-d6) 9.16 (1H, br s), 9.01 (1H, br s), 7.90 (1H, br s), 7.64 (1H, d, J 11.0 Hz), 7.50 (1H, d, J 8.6 Hz), 7.17 (1H, t, J 8.8 Hz), 4.42 (2H, brs), 2.88 (2H, d, J 4.7 Hz), 2.71 (2H, s), 0.96 (6H, s). LCMS (ES+) RT 2.62 minutes, 489 (M+H)+.
Example 2 (250 mg, 0.51 mmol), EDC (98 mg, 0.51 mmol), HOBT (69 mg, 0.51 mmol), NMM (103 mg, 1.02 mmol) and 2-(3H-imidazol-4-yl)ethylamine (62 mg, 0.56 mmol) in DMF (5 mL) were stirred at r.t. for 18 h. The reaction was treated with water and DCM, stirred and the organic phase separated then concentrated in vacuo. Residual water and DMF were removed by azeotrope with heptane and the resulting oil chromatographed (SiO2, DCM-ethyl acetate) to yield the title compound. δH (DMSO-d6) 9.64 (1H, br s), 8.17 (1H, s), 8.01 (1H, t, J 4.8 Hz), 7.87 (1H, t, J 4.7 Hz), 7.65 (1H, dd, J 1.9, 10.6 Hz), 7.53-7.49 (2H, m), 7.20 (1H, t J 8.7 Hz), 6.81 (1H, s), 3.44-3.38 (2H, m), 2.86 (2H, d, J 4.9 Hz), 2.68-2.63 (4H, m), 0.93 (6H, s). LCMS (ES+) RT 1.97 minutes, 568 (M+H)+.
Prepared by the method of Example 32, starting from (S)-(2,2-dimethyl-[1,3]dioxolan-4-yl)methylamine (75 mg, 0.56 mmol). δH (DMSO-d6) 9.31 (1H, s), 8.00 (1H, s), 7.85 (1H, m), 7.73 (1H, d, J 10.7 Hz), 7.59 (1H, d, J 8.6 Hz), 7.26 (1H, t, J 8.8 Hz), 4.86 (1H, d, J 4.9 Hz), 4.64 (1H, t, J 5.8 Hz), 3.68-3.62 (1H, m), 3.46-3.40 (3H, m), 3.26-3.18 (1H, m), 2.96 (2H, d, J 4.9 Hz), 2.83 (2H, s), 1.06 (6H, s). LCMS (ES+) RT 2.63 minutes, 548 (M+H)+.
Prepared by the method of Example 32, starting from 1-methylpiperidin-4-ylamine (64 mg, 0.56 mmol). δH (DMSO-d6) 8.77 (1H, br s), 7.93 (1H, br s), 7.85 (1H, br s), 7.61 (1H, dd, J 10.7, 1.7 Hz), 7.45 (1H, d, J 8.5 Hz), 7.03 (1H, t, J 8.8 Hz), 3.59 (1H, br s), 2.86 (2H, d, J 5.0 Hz), 2.73-2.64 (4H, m), 2.15 (3H, s), 2.01-1.91 (2H, m), 1.65-1.61 (2H, m), 1.48-1.35 (2H, m), 0.96 (6H, s). LCMS (ES+) RT 2.27 minutes, 571 (M+H)+.
Prepared by the method of Example 32, starting from (S)-pyrrolidin-3-ol (49 mg, 0.56 mmol). δH (DMSO-d6) 8.59-8.43 (1H, m), 7.92-7.81 (1H, m), 7.59 (1H, dd, J 10.6, 1.6 Hz), 7.42 (1H, dd, J 8.5, 1.0 Hz), 6.96 (1H, t, J 8.7 Hz), 4.88 (1H, br s), 4.20 (1H, br s), 3.50-2.99 (5H, m), 2.92-2.79 (2H, m), 2.49-2.34 (1H, m), 1.93-1.62 (2H, m), 0.95 (6H, s). LCMS (ES+) RT 2.62 minutes, 544 (M+H)+.
Prepared by the method of Example 32, starting from (S)-(pyrrolidin-2-yl)-methanol (57 mg, 0.56 mmol). δH (DMSO-d6) 8.40-7.70 (1H, br s), 7.52 (1H, d, J 9.3 Hz), 7.43 (1H, d, J 8.4 Hz), 7.38 (1H, br s), 7.03 (1H, t, J 8.7 Hz), 4.40-4.10 (1H, br s), 4.01-3.94 (1H, br m), 3.47-3.39 (1H, m), 3.39-3.27 (3H, m), 3.01-2.88 (2H, m), 2.66-2.56 (2H, m), 1.94-1.79 (3H, m), 1.78-1.63 (1H, m), 1.02 (3H, s), 0.99 (3H, s). LCMS (ES+) RT 2.82 minutes, 558 (M+H)+.
Prepared by the method of Example 32, starting from 3-aminopropan-1-ol (42 mg, 0.56 mmol). δH (DMSO-d6) 9.10 (1H, br s), 7.90 (2H, br s), 7.60 (1H, d, J 10.6 Hz), 7.47 (1H, d, J 8.6 Hz), 7.12 (1H, t, J 8.8 Hz), 4.45 (1H, t, J 5.0 Hz), 3.42 (2H, q, J 6.0 Hz), 3.22 (2H, q, J 6.7 Hz), 2.87 (2H, d, J 5.0 Hz), 2.72 (2H, s), 1.56 (2H, d, J 6.5 Hz), 0.96 (6H, s). LCMS (ES+) RT 2.83 minutes, 532 (M+H)+.
Prepared by the method of Example 32, starting from (R)-3-Aminoxypropane-1,2-diol (83 mg, 0.56 mmol). δH (DMSO-d6) 11.24 (1H, br s), 8.86 (1H, br s), 7.91 (1H, br s), 7.63 (1H, dd, J 10.6, 1.9 Hz), 7.48 (1H, d, J 8.5 Hz), 7.10 (1H, t, J 8.7 Hz), 4.86 (1H, br s), 4.57 (1H, br s), 3.80 (1H, q, J 7.1 Hz), 3.72-3.61 (2H, m), 3.35 (2H, d, J 4.7 Hz), 2.86 (2H, d, J 5.1 Hz), 2.66 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.46 minutes, 564 (M+H)+.
Intermediate 18 (200 mg, 0.31 mmol) was dissolved in DCM (5 mL) and treated with 4M HCl in 1,4-dioxane (5 mL), after stirring at r.t. for 2 h. The solvent was removed in vacuo and the residue triturated with methanol, yielding the required product. δH (DMSO-d6) 9.10 (1H, s), 8.01-7.93 (2H, m), 7.80 (2H, br s), 7.63 (1H, dd, J 10.7, 1.9 Hz), 7.47 (1H, dd, J 8.5, 1.0 Hz), 7.11 (1H, t, J 8.8 Hz), 3.16 (2H, q, J 6.4 Hz), 2.88 (2H, d, J 5.0 Hz), 2.76 (2H, br s), 2.72 (2H, s), 1.60-1.38 (4H, m), 0.97 (6H, s). LCMS (ES+) RT 2.36 minutes, 545 (M+H)+.
Intermediate 20 (120 mg, 0.22 mol) was dissolved in 2-(2-ethoxyethoxy)ethanol (7.5 mL) and liquid ammonia (10 mL) added. The reaction was heated in a Parr instrument to 90° C. at 60 atmospheres for 24 h. The residue was dissolved in DCM (100 mL) and washed with water (100 mL). The combined organic extracts were dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 9% MeOH, 1% aqueous NH3 and 89% DCM) to give the title compound as a yellow solid (37 mg, 34%). δH (DMSO-d6) 9.90 (1H, d, J 4.0 Hz), 8.35-8.32 (1H, m), 7.70 (1H, d, J 2.0 Hz), 7.53 (1H, dd, J 8.5, 2.0 Hz), 7.32 (2H, br s), 7.17 (1H, d, J 8.5 Hz), 6.60 (1H, d, J 4.3 Hz), 3.54-3.34 (4H, m), 2.80 (2H, s), 0.98 (6H, s). LCMS (ES+) RT 2.44 minutes, 488.8 (M+H)+.
Chromatography of the crude residue from the preparation of Example 40 (silica, 9% MeOH, 1% aqueous NH3 and 89% DCM) gave the title compound as a yellow solid (11 mg, 10%). δH (DMSO-d6) 10.50 (1H, s), 9.76-9.73 (1H, m), 8.72 (1H, br s), 8.00 (1H, d, J 1.6 Hz), 7.86 (1H, d, J 8.5 Hz), 7.49 (1H, dd, J 8.5, 2.0 Hz), 4.36 (2H, q, J 7.0 Hz), 2.93-2.89 (4H, m), 1.35 (3H, t, J 7.0 Hz), 1.00 (6H, s). LCMS (ES+) RT 2.78 minutes, 517.8 (M+H)+.
Example 1(1 g, 2 mmol) and lithium hydroxide (115 mg, 4.8 mmol) were added to a solution of THF/H2O (150 mL, 2:1) and stirred at 110° C. for 18 h. The crude reaction was acidified (pH=1) using 2N HCl and the mixture stirred at 95° C. until decarboxylation was completed. The reaction was filtered and the filtrate extracted with DCM (3×100 mL). The combined organic extracts were washed with water (300 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was triturated with diethyl ether (7 mL) to give the title compound as a yellow solid (732 mg, 85%). δH (DMSO-d6) 9.00 (1H, s), 7.68-7.65 (1H, m), 7.59 (1H, dd, J 10.8, 2.0 Hz), 7.46 (1H, d, J 8.5 Hz), 7.27-7.20 (1H, m), 6.36 (1H, s), 2.89 (2H, d, J 5.0 Hz), 2.60 (2H, s), 0.96 (6H, s). LCMS (ES+) RT 3.49 minutes, 431 (M+H)+.
Prepared from Intermediate 23 (98 mg, 0.19 mmol) by the method of Example 6. The title compound was obtained as a yellow solid (10 mg, 11%). δH (DMSO-d6) 9.50 (1H, s), 7.99 (1H, m), 7.83 (1H, m), 7.59 (1H, d, J 1.5 Hz), 7.40 (2H, m), 4.76 (1H, d, J 4.9 Hz), 4.54 (1H, t, J 5.7 Hz), 4.16 (1H, s), 3.57-3.55 (1H, m), 3.33-3.29 (3H, m), 3.18-3.12 (1H, m), 2.89 (2H, d, J 4.5 Hz), 2.78 (2H, s), 0.98 (6H, s). LCMS (ES+) RT 2.45 minutes, 462 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 1-aminopropan-2-ol (59 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (151 mg, 73%). δH (DMSO-d6) 8.87 (1H, s), 7.92 (1H, t, J 4.9 Hz), 7.67 (1H, s), 7.61 (1H, dd, J 10.7, 1.9 Hz), 7.46 (1H, d, J 8.5 Hz), 7.07 (1H, t, J 8.8 Hz), 4.71 (1H, t, J 5.5 Hz), 3.90-3.86 (1H, m), 3.38-3.21 (2H, m), 2.86 (2H, d, J 5.0 Hz), 2.70 (2H, s), 0.99 (3H, d, J 6.7 Hz), 0.96 (6H, s). LCMS (ES+) RT 2.78 minutes, 532 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and morpholine (70 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (35 mg, 17%). δH (DMSO-d6) 8.57 (1H, s), 7.89 (1H, br s), 7.61 (1H, d, J 10.4 Hz), 7.41 (1H, d, J 8.0 Hz), 6.95 (1H, t, J 8.5 Hz), 3.46-3.29 (10H, m), 2.87 (2H, s), 0.95 (6H, s). LCMS (ES+) RT 2.55 minutes, 542 (M+H)−.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and pyridin-2-ylhydrazine (85 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (24 mg, 11%). δH (DMSO-d6) 9.85 (2H, br s), 8.78 (1H, s), 7.97 (1H, d, J 4.5 Hz), 7.89-7.84 (1H, m), 7.72 (1H, dd, J 10.4, 1.5 Hz), 7.58-7.50 (2H, m), 7.40 (1H, t, J=8.9 Hz), 6.74-6.66 (2H, m), 2.90 (2H, d, J 4.9 Hz), 2.70 (2H, s), 0.98 (6H, s). LCMS (ES+) RT 2.85 minutes, 566 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 2-(piperazin-1-yl)ethanol (102 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (98 mg, 45%). δH (DMSO-d6) 8.52 (1H, s), 7.89 (1H, t, J 4.6 Hz), 7.60 (1H, dd, J 10.5, 1.7 Hz), 7.40 (1H, d, J 8.5 Hz), 6.92 (1H, t, J 8.7 Hz), 4.36 (1H, t, J 5.1 Hz), 3.48-3.43 (2H, m), 3.38-3.30 (1H, m), 2.86 (2H, d, J 4.5 Hz), 2.50-2.46 (5H, m), 2.35-2.29 (6H, m), 0.95 (6H, s). LCMS (ES+) RT 2.44 minutes, 587 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 3-aminopropionic acid ethyl ester (120 mg, 1.02 mmol) by the method of Example 19. The title compound was obtained as a cream solid (82 mg, 37%). δH (DMSO-d6) 9.08 (1H, s), 7.98-7.91 (2H, m), 7.63 (1H, d, J 10.6 Hz), 7.49 (1H, d, J 8.3 Hz), 7.12 (1H, t, J 8.8 Hz), 4.04 (2H, q, J 7.0 Hz), 3.38 (2H, d, J 5.8 Hz), 2.86 (2H, d, J 4.0 Hz), 2.68 (2H, s), 2.47-2.43 (2H, s), 1.17 (3H, t, J 7.0 Hz), 0.95 (6H, s). LCMS (ES+) RT 3.27 minutes, 574 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (azetidin-3-ylmethyl)-carbamic acid tert-butyl ester (145 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (236 mg, 94%). δH (DMSO-d6) 8.70 (1H, s), 7.86 (1H, t, J 5.0 Hz), 7.63 (1H, dd, J 10.5, 1.9 Hz), 7.46 (1H, d, J 8.5 Hz), 7.01 (1H, t, J=8.7 Hz), 6.98-6.95 (1H, m), 4.02-3.85 (2H, m), 3.59 (2H, dd, J 9.2, 5.3 Hz), 3.30-3.27 (1H, m), 3.08-3.04 (2H, m), 2.85 (2H, d, J 5.1 Hz), 2.56 (2H, s), 1.35 (9H, s), 0.96 (6H, s). LCMS (ES+) RT 3.14 minutes, 643 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and (R)-1-BOC-3-amino-pyrrolidine (145 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (207 mg, 83%). δH (DMSO-d6) 8.80 (1H, br s), 8.17 (1H, br s), 7.93 (1H, br s), 7.60 (1H, d, J 9.9 Hz), 7.46 (1H, d, J 8.3 Hz), 7.09-7.01 (1H, m), 4.25-4.20 (1H, m), 3.38 (1H, dd, J 10.7, 6.2 Hz), 3.29-3.26 (1H, m), 3.15-3.06 (1H, m), 2.86 (2H, d, J 4.9 Hz), 2.66 (2H, s), 1.99-1.90 (1H, m), 1.81-1.69 (1H, m), 1.39 (10H, s), 0.95 (6H, d, J 3.4 Hz). LCMS (ES+) RT 3.38 minutes, 643 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and [1,4]diazepane (78 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (118 mg, 54%). δH (DMSO-d6; 120° C.) 7.52 (1H, dd, J 10.6, 1.8 Hz), 7.43 (2H, d, J=8.5 Hz), 7.05 (1H, t, J 8.7 Hz), 3.60-3.52 (1H, m), 3.44-3.39 (3H, m), 2.94 (2H, d, J 5.1 Hz) 2.84-2.76 (5H, m), 2.53 (2H, s), 1.68-1.60 (2H, m), 1.00 (6H, s). One exchangeable proton was not observed. LCMS (ES+) RT 2.34 minutes, 557 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and N,N-dimethylethylene-diamine (69 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (115 mg, 54%). δH (DMSO-d6) 9.22 (1H, br s), 7.88-7.84 (2H, m), 7.66 (1H, dd, J 10.5, 1.3 Hz), 7.50 (1H, d, J 8.3 Hz), 7.14 (1H, t, J 8.7 Hz), 3.29-3.26 (2H, m), 2.88 (2H, d, J 4.7 Hz), 2.71 (2H, s), 2.27 (2H, t, J 6.1 Hz), 2.14 (6H, s), 0.96 (6H, s). LCMS (ES+) RT 2.52 minutes, 545 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 2-(morpholin-4-yl)-ethylamine (102 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (136 mg, 60%). δH (DMSO-d6) 9.16 (1H, s), 7.92 (1H, t, J 4.7 Hz), 7.78 (1H, t, J 5.3 Hz), 7.65 (1H, dd, J 10.5, 1.7 Hz), 7.50 (1H, d, J 8.7 Hz), 7.13 (1H, t, J 8.8 Hz), 3.52-3.49 (2H, m), 3.30 (2H, d, J 5.6 Hz), 2.89 (2H, d, J 4.9 Hz), 2.74 (2H, s), 2.49-2.30 (6H, m), 2.07 (2H, s), 0.98 (6H, s). LCMS (ES+) RT 2.55 minutes, 587 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and N,N,2,2-tetramethyl-1,3-propanediamine (102 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (124 mg, 54%). δH (DMSO-d6) 9.10 (1H, br s), 8.00-7.92 (2H, m), 7.62 (1H, dd, J 10.7, 1.9 Hz), 7.48 (1H, d, J 8.5 Hz), 7.09 (1H, t, J 8.7 Hz), 3.09 (2H, s), 2.89 (2H, d, J 4.9 Hz), 2.76 (2H, s), 2.25-2.05 (8H, m), 0.97 (6H, s), 0.82 (6H, s). LCMS (ES+) RT 2.56 minutes, 587 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 3-(4-methylpiperazin-1-yl)propylamine (123 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (96 mg, 40%). δH (DMSO-d6) 8.70 (1H, br s), 7.68 (1H, br s), 7.35 (1H, d, J 10.7 Hz), 7.21 (1H, d, J 8.6 Hz), 6.80 (1H, t, J 8.8 Hz), 3.05 (2H, s), 2.90 (2H, t, J 6.5 Hz), 2.63 (2H, d, J 4.8 Hz), 2.46 (2H, s), 2.14-1.96 (9H, m),1.89 (3H, s), 1.30-0.99 (2H, m), 0.72 (6H, s). LCMS (ES+) RT 2.43 minutes, 614 (M+H)+.
Trifluoroacetic acid (2 mL) was added to a solution of Intermediate 24 (224 mg, 0.34 mmol) in DCM (2 mL) and the reaction mixture was stirred at r.t. for 18 h. Saturated aqueous NaHCO3 solution was added to the reaction until pH=8 and the aqueous fraction was extracted with DCM (3×100 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 10% MeOH in DCM) to give the title compound as a yellow solid (128 mg, 68%). δH (DMSO-d6) 9.10 (1H, br s), 7.72 (1H, br s), 7.56 (1H, d, J 10.7 Hz), 7.45 (1H, d, J 8.7 Hz), 7.18-7.12 (1H, m), 3.40-3.25 (3H, m), 2.90-2.80 (6H, m), 1.75-1.60 (3H, m), 1.52-1.41 (1H, m), 0.96 (6H, s). Two exchangeable protons were not observed. LCMS (ES+) RT 2.55 minutes, 557 (M+H)+.
Prepared from Intermediate 25 (236 mg, 0.36 mmol) by the method of Example 56 to give the title compound as a yellow solid (117 mg, 56%). δH (DMSO-d6) 9.19 (1H, br s), 7.70 (1H, br s), 7.56 (1H, d, J 10.7 Hz), 7.45 (1H, d, J 8.7 Hz), 7.16 (1H, t, J 8.7 Hz), 3.35-3.26 (3H, m), 2.89-2.78 (6H, m), 1.76-1.68 (3H, m), 1.43-1.38 (1H, m), 0.96 (6H, s). Two exchangeable protons were not observed. LCMS (ES+) RT 2.54 minutes, 557 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 1-methylpiperazine (90 mg, 0.90 mmol) by the method of Example 19. The title compound was obtained as a cream solid (32 mg, 15%). δH (DMSO-d6) 8.51 (1H, s), 7.89 (1H, br s), 7.60 (1H, d, J 10.6 Hz), 7.40 (1H, d, J 8.3 Hz), 6.91 (1H, t, J 8.6 Hz), 2.87-2.83 (2H, m), 2.58-2.45 (6H, m), 2.17-2.12 (7H, m), 0.94 (6H, s). LCMS (ES+) RT 2.36 minutes, 557 (M+H)+.
Prepared from Intermediate 26 (149 mg, 0.23 mmol) by the method of Example 7 to give the title compound as a yellow solid (82 mg, 61%). δH (DMSO-d6) 9.39 (1H, br s), 9.23 (1H, br s), 8.98 (1H, s), 8.39 (1H, d, J 6.3 Hz), 7.93 (1H, br s), 7.62 (1H, d, J 10.2 Hz), 7.48 (1H, d, J 7.8 Hz), 7.09 (1H, t, J 8.5 Hz), 4.49-4.30 (1H, m), 3.39-3.14 (3H, m), 3.55-3.42 (1H, m), 3.09-3.02 (1H, m), 2.88 (2H, d, J 3.4 Hz), 2.73 (2H, s), 2.11-2.04 (1H, m), 1.81-1.72 (1H, m), 0.97 (6H, s). LCMS (ES+) RT 2.71 minutes, 542 (M+H)+.
Prepared from Example 50 (192 mg, 0.30 mmol) by the method of Example 7 to give the title compound as a yellow solid (54 mg, 33%). δH (DMSO-d6) 9.30 (1H, br s), 9.16 (1H, br s), 8.89 (1H, s), 8.31 (1H, d, J 6.1 Hz), 7.85 (1H, br s), 7.54 (1H, d, J 10.6 Hz), 7.39 (1H, d, J 7.8 Hz), 7.00 (1H, t, J 8.6 Hz), 4.40-4.30 (1H, m), 4.11-3.95 (1H, m), 3.39-3.14 (4H, m), 2.96-2.73 (2H, m), 2.64 (2H, s), 2.00-1.92 (1H, m), 1.73-1.65 (1H, m), 0.88 (6H, s). LCMS (ES+) RT 2.72 minutes, 543 (M+H)+.
Prepared from Intermediate 5 (250 mg, 0.39 mmol) and 3-(aminomethyl)-azetidine-1-carboxylic acid tert-butyl ester (145 mg, 0.78 mmol) by the method of Example 19. The title compound was obtained as a cream solid (211 mg, 84%). δH (DMSO-d6) 8.99 (1H, s), 8.11 (1H, t, J 5.3 Hz), 7.94 (1H, t, J 4.7 Hz), 7.63 (1H, dd, J 10.7, 1.9 Hz), 7.48 (1H, d, J 8.5 Hz), 7.09 (1H, t, J 8.7 Hz), 3.80 (2H, t, J 8.3 Hz), 3.53 (2H, dd, J 8.3, 5.5 Hz), 3.34 (2H, t, J 6.2 Hz), 2.88 (2H, d, J 4.9 Hz), 2.70 (2H, s), 2.65-2.61 (1H, m), 1.36 (9H, s), 0.96 (6H, s). LCMS (ES+) RT 3.21 minutes, 641 (M+H)−.
Prepared from Example 61 (251 mg, 0.39 mmol) and trifluoroacetic acid by the method of Example 56. The title compound was obtained as a yellow solid (9 mg, 4%). δH (DMSO-d6) 8.38 (1H, s), 7.67 (1H, s), 7.58 (1H, dd, J 10.5, 1.7 Hz), 7.48 (1H, dd, J 8.5, 1.1 Hz), 7.15 (1H, t, J 8.8 Hz), 3.49-3.42 (4H, m), 3.15 (2H, dd, J 13.0, 8.4 Hz), 2.85 (2H, d, J 4.7 Hz), 2.66 (2H, s), 2.08-1.95 (1H, m), 1.23-1.15 (2H, m), 0.97 (6H, s). LCMS (ES+) RT 2.49 minutes, 543 (M+H)+.
Prepared from Example 49 (463 mg, 0.72 mmol) and trifluoroacetic acid by the method of Example 56. The title compound was obtained as a yellow solid (254 mg, 65%). δH (DMSO-d6) 7.78 (1H, t, J 4.8 Hz), 7.64 (1H, dd, J 10.6, 1.8 Hz), 7.45 (1H, d, J=8.5 Hz), 7.00 (1H, t, J 8.7 Hz), 5.26 (2H, br s), 3.91 (2H, t, J 8.7 Hz), 3.62-3.57 (2H, m), 2.85 (2H, d, J 5.0 Hz), 2.71 (2H, d, J 7.0 Hz), 2.56 (2H, s), 0.96 (6H, s). LCMS (ES+) RT 2.39 minutes, 543 (M+H)+.
Prepared from Intermediate 5 (350 mg, 0.55 mmol) and racemic 2-(aminomethyl)-piperidine-1-carboxylic acid tert-butyl ester (233 mg, 1.09 mmol) by the method of Example 19. The title compound was obtained as a cream solid (304 mg, 83%). δH (DMSO-d6) 9.31 (1H, br s), 7.93 (1H, t, J 4.7 Hz), 7.85-7.80 (1H, m), 7.64 (1H, dd, J 10.7, 1.9 Hz), 7.50 (1H, d, J 8.5 Hz), 7.15 (1H, t, J 8.7 Hz), 4.28-4.21 (1H, m), 3.83-3.79 (1H, m), 3.42-3.29 (2H, m), 2.88 (3H, d, J 4.9 Hz), 2.73 (2H, d, J 4.0 Hz), 1.58-1.38 (5H, m), 1.30 (10H, s), 0.97 (6H, s). LCMS (ES+) RT 3.62 minutes, 671 (M+H)+.
Prepared from Intermediate 5 (350 mg, 0.55 mmol) and N-(2-aminoethyl)-N-methylcarbamic acid tert-butyl ester (142 mg, 1.09 mmol) by the method of Example 19. The title compound was obtained as a cream solid (300 mg, 87%). δH (DMSO-d6) 9.33-9.20 (1H, m), 7.93-7.90 (2H, m), 7.65 (1H, dd, J 10.7, 1.9 Hz), 7.50 (1H, d, J 8.5 Hz), 7.15 (1H, t, J 8.5 Hz), 3.29-3.24 (4H, m), 2.88 (2H, d, J 4.9 Hz), 2.79 (3H, s), 2.73 (2H, s), 1.33 (9H, s), 0.97 (6H, s). LCMS (ES+) RT 3.42 minutes, 631 (M+H)+.
Prepared from Example 65 (273 mg, 0.43 mmol) by the method of Example 7 to give the title compound as a yellow solid (196 mg, 80%). δH (DMSO-d6) 9.37 (1H, s), 8.94 (2H, br s), 8.20 (1H, br s), 7.93 (1H, d, J 0.4 Hz), 7.64 (1H, d, J 10.4 Hz), 7.51 (1H, d, J 8.3 Hz), 7.19 (1H, t, J 8.5 Hz), 3.47 (2H, d, J 4.6 Hz), 2.95-2.88 (4H, m), 2.78 (2H, s), 2.49 (3H, s), 0.97 (6H, s). LCMS (ES+) RT 2.42 minutes, 530 (M+H)+.
Prepared from Example 64 (284 mg, 0.42 mmol) by method of Example 7 to give the title compound as a yellow solid (157 mg, 66%). δH (DMSO-d6) 9.24 (1H, s), 9.10 (1H, br s), 8.89-8.86 (1H, m), 8.28 (1H, br s), 7.95 (1H, br s), 7.63 (1H, dd, J 10.7, 1.9 Hz), 7.48 (1H, d, J 8.3 Hz), 7.13 (1H, t, J 8.7 Hz), 4.01-3.90 (1H, m), 3.69-3.51 (1H, m), 3.48-3.36 (1H, m), 3.29-3.17 (1H, m), 3.10-2.98 (1H, m), 2.88 (2H, d, J 4.0 Hz), 2.77-2.73 (2H, m), 1.71-1.64 (4H, m), 1.39-1.23 (2H, m), 0.97 (6H, s). LCMS (ES+) RT 2.94 minutes, 571 (M+H)+.
Paraformaldehyde (63 mg, 2.10 mmol) was added to a solution of Example 23 (250 mg, 0.42 mmol) and sodium cyanoborohydride (32 mg, 0.51 mmol) in MeOH (5 mL) and the reaction mixture was stirred at r.t. for 18 h. 2M HCl (20 mL) was added to the reaction which was then neutralised with NaOH (10% aq) and extracted with DCM (3×100 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-10% MeOH in DCM) to give the title compound as a white solid (15 mg, 60%). δH (DMSO-d6) 8.86 (1H, br s), 7.93 (1H, br s), 7.73 (1H, d, J 7.5 Hz), 7.61 (1H, dd, J 10.7, 1.7 Hz), 7.45-7.42 (1H, m), 7.00 (1H, t, J 8.8 Hz), 3.80-3.77 (1H, m), 2.86 (2H, d, J 5.1 Hz), 2.68 (2H, s), 2.49-2.46 (2H, m), 2.11 (3H, s), 1.95-1.92 (1H, m), 1.89-1.72 (1H, m), 1.60-1.52 (2H, m), 1.48-1.40 (1H, m), 1.25-1.17 (1H, m), 0.96 (6H, d, J 2.0 Hz). LCMS (ES+) RT 2.43 minutes, 571 (M+H)+.
Prepared from Intermediate 27 (367 mg, 0.58 mmol) and trifluoroacetic acid by the method of Example 56. The title compound was obtained as a cream solid (215 mg, 70%). δH (DMSO-d6) 7.86 (1H, t, J 4.9 Hz), 7.64 (1H, dd, J 10.6, 1.9 Hz), 7.48-7.45 (1H, m), 7.04 (1H, t, J 8.7 Hz), 4.02 (2H, t, J 8.5 Hz), 3.65-3.50 (3H, m), 2.85 (2H, d, J=5.0 Hz), 2.56 (2H, s), 0.95 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT 1.97 minutes, 529 (M+H)+.
Prepared from Intermediate 28 (212 mg, 0.33 mmol) and trifluoroacetic acid by the method of Example 19. The title compound was obtained as a cream solid (18 mg, 10%). δH (DMSO-d6) 7.94 (1H, t, J 4.9 Hz), 7.75 (1H, dd, J 10.7, 1.8 Hz), 7.64 (1H, d, J 8.5 Hz), 7.47 (1H, t, J 8.7 Hz), 4.42 (1H, t, J 8.0 Hz), 4.29-4.16 (2H, m), 2.92 (2H, s), 2.87 (2H, d, J 5.0 Hz), 2.71 (2H, d, J 5.0 Hz), 0.98 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT 2.05 minutes, 529 (M+H)+.
Intermediate 32 (240 mg, 0.37 mmol) was dissolved in DCM (5 mL) and TFA (2.5 mL) was added. The reaction mixture was stirred overnight and then quenched by the addition of 10% aqueous NaOH. The DCM was separated and the aqueous layer was extracted with DCM (2×25 mL). The combined organics were dried with MgSO4 and the solvent was removed under reduced pressure. The resulting oil was purified on silica (DCM DCM/MeOH 2.5%) followed by preparative HPLC to yield the title compound as an off-white solid (9 mg, 6%). δH (d6-DMSO) 7.63-7.56 (1H, m), 7.45-7.39 (1H, m), 6.98 (1H, t, J 8.9 Hz), 3.93-3.85 (2H, m), 3.63-3.54 (2H, m), 3.30 (2H, s), 3.06 (2H, s), 3.04 (3H, s), 2.59 (2H, m), 0.98 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT (pH 3) 2.17 minutes, 557 (M+H)+.
Intermediate 33 (200 mg, 0.86 mmol) was dissolved in DCM (10 mL) and trifluoroacetic acid (1 mL) was added. The reaction mixture was stirred for 1 h and then the solvent and remaining acid were removed under vacuum at room temperature. The resulting salt was dissolved in DMF (8.3 mL) and Intermediate 5 (353 mg, 0.55 mmol) and triethylamine (317 μL) were added sequentially. The reaction was stirred for 18 h and the DMF was then removed under reduced pressure. A saturated aqueous solution of NaHCO3 was then added to the pale solid and the biphasic system was stirred for 1 h. The remaining solid was filtered, washed with further NaHCO3 (aq) and dried. This was then stirred in 50:50 MeCN/isopropyl ether, filtered once more and dried in a vacuum oven at 50° C. to yield the title compound (128 mg, 40%). δH (d6-DMSO) 8.71 (1H, s), 7.86 (1H, t, J 5.1 Hz), 7.67 (1H, dd, J 10.5, 1.9 Hz), 7.53-7.47 (1H, m), 7.08 (1H, t, J 8.7 Hz), 6.48 (1H, s), 4.82 (2H, s), 4.16 (2H, d, J 10.2 Hz), 3.87 (2H, d, J 10.2 Hz), 2.86 (2H, d, J 5.1 Hz), 2.59 (2H, s), 0.96 (6H, s). LCMS (ES+) RT (pH 10) 2.32 minutes, 589 (M+H)+.
Intermediate 34 was reacted according to method of Example 72 to yield the title compound (250 mg, 81%). δH (d6-DMSO) 7.88 (1H, t, J 5.1 Hz), 7.63 (1H, dd, J 10.5, 1.9 Hz), 7.48-7.43 (1H, m), 7.01 (1H, t, J 8.7 Hz), 5.66 (1H, br s), 4.58 (2H, br s), 3.86 (2H, d, J 9.8 Hz), 3.67 (2H, d, J 9.8 Hz), 2.85 (2H, d, J 5.1 Hz), 2.62 (2H, s), 2.56 (2H, s), 0.96 (6H, s). One exchangeable proton was not observed. LCMS (ES+) RT (pH 10) 1.87 minutes, 559 (M+H)+.
Intermediate 34 (100 mg, 0.50 mmol) was dissolved in DMF (4.5 mL) and
Intermediate 5 (289 mg, 0.45 mmol) and triethylamine (70 μL) were added sequentially. The reaction mixture was stirred overnight and the DMF was removed under reduced pressure. The resulting solid was dissolved in DCM (25 mL) and extracted with aq NaHCO3 (25 mL), aq citric acid (25 mL) and brine (25 mL). The organic layer was dried with sodium sulfate and the solvent removed to yield a pale solid. This was purified using silica gel chromatography (DCM to DCM/MeOH 5%) to yield a white solid which was triturated with 50:50 MeCN/isopropyl ether and dried to yield the title compound (198 mg, 73%). δH (d6-DMSO) 9.06 (1H, s), 8.07-8.03 (1H, m), 7.95-7.90 (1H, m), 7.64 (1H, d, J 12.2 Hz), 7.52-7.48 (1H, m), 7.15 (1H, t, J 9.0 Hz), 5.86 (1H, s), 3.82 (2H, d, J 9.0 Hz), 3.62-3.57 (2H, m), 3.41-3.37 (2H, m), 2.88 (2H, d, J 4.8 Hz), 2.73 (2H, s), 1.35 (9H, s), 0.95 (6H, s). LCMS (ES+) RT (pH 10) 2.02 minutes, 659 (M+H)+.
Example 74 (155 mg, 0.24 mmol) was dissolved in DCM (2.4 mL) and TFA (0.25 mL) was added. The reaction was stirred overnight and the solvent and acid were removed. The amine was then purified with silica gel chromatography (DCM to DCM/MeOH 5%) and preparative HPLC to yield the title compound (15 mg, 11%). δH (d6-DMSO) 9.09 (1H, s), 7.66-7.62 (1H, m), 7.58 (1H, dd, J 10.5, 1.9 Hz), 7.50-7.45 (1H, m), 7.23 (1H, t, J 8.9 Hz), 6.90 (1H, br s), 3.55-3.47 (4H, m), 3.40 (2H, s), 2.83 (2H, d, J 4.9 Hz), 2.78 (2H, s), 0.95 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT (pH 10) 2.02 minutes, 559 (M+H)+.
Intermediate 39 (2.40 g, 7.13 mmol), Intermediate 2 (1.67 g, 7.13 mmol) and cesium carbonate (2.32 g, 7.13 mmol) in DMF (20 mL) were heated at 80° C. for 2 h. The reaction mixture was poured onto ice-water (200 mL) and acidified with 2M aqueous HCl. The resulting orange precipitate was filtered off, washed with water then acetonitrile and dried in vacuo yielding the title compound (1.70 g, 38%). δH (DMSO-d6) 9.79 (1H, s), 7.95-7.91 (2H, m), 7.73 (1H, dd, J 8.4 Hz), 7.21 (1H, d, J 8.4 Hz), 2.93 (2H, d, J 5.0 Hz), 2.63 (2H, s), 0.99 (6H, s). LCMS (ES+) RT 3.13 minutes, 472/474 (M+H)+.
Intermediate 6 (1 g, 3.55 mmol), 3-chloro-4-fluorobenzonitrile (0.55 g, 3.55 mmol) and cesium carbonate (1.15 g, 3.55 mmol) in DMF (10 mL) were stirred at 65° C. overnight, then partitioned between DCM (200 mL) and water (200 mL). The organic phase was separated, dried over sodium sulphate and concentrated. After azeotroping with heptane to remove residual DMF the product was subjected to column chromatography (SiO2; DCM/ethyl acetate) to yield the title compound (800 mg, 54%). δH (DMSO-d6) 10.98 (1H, s), 8.17 (1H, d, J 1.9 Hz), 8.13 (1H, t, J 5.0 Hz), 7.90 (1H, dd, J 8.6, 1.9 Hz), 7.80 (1H, d, J 8.6 Hz), 4.33 (2H, q, J 7.1 Hz), 2.93 (2H, s), 2.87 (2H, d, J 5.1 Hz), 1.32 (3H, t, J 7.1 Hz), 1.00 (6H, s). LCMS (ES+) RT 3.31 minutes, 418/420 (M+H)+.
Propylamine (140 μL, 1.70 mmol) in toluene (3 mL) was treated with 2M trimethylaluminium in toluene (852 μL, 1.70 mmol) and the reaction mixture stirred at r.t. for 0.5 h. Example 76 (140 mg, 0.34 mmol) in toluene (2 mL) was added and the reaction mixture stirred at reflux for 3.5 h. The reaction was then cooled to r.t. and poured onto a slurry of silica in DCM (6 mL) and methanol (2 mL). After filtering and washing with further DCM/methanol the filtrate was concentrated in vacuo and dried at 50° C. under vacuum yielding the title compound (137 mg, 15%). δH (DMSO-d6) 7.90 (1H, br m), 7.71 (1H, s), 7.55 (1H, d, J 8.5 Hz), 7.20 (1H, d, J 8.5 Hz), 3.20-3.10 (2H, brm), 2.86 (2H, br m), 2.78-2.73 (2H, br m), 1.62-1.55 (2H, br m), 0.98 (6H, s), 0.93 (3H, t, J 7.4 Hz). Some exchangeable protons were not observed. LCMS (ES+) RT 2.55 minutes, 531/533 (M+H)+.
From Example 76 (300 mg, 0.64 mmol), 2M trimethylaluminium in toluene (1.6 mL, 3.18 mmol) and 1,2-diamino-2-methylpropane (280 mg, 3.18 mmol) in toluene (5 mL) by the method of Example 78. After pouring onto a mixture of silica (1 g) and 2:1 DCM/methanol (50 mL) and washing with further DCM/methanol the filtrate was concentrated to yield an orange solid (400 mg). The residue was dissolved in DCM and washed with 2M NaOH, dried and concentrated. Chromatography (silica; DCM→1:1 DCM:ethyl acetate) yielded the title compound (35 mg, 10%). δH (DMSO-d6) 11.21 (1H, br s), 8.70 (1H, br s), 7.74 (1H, d, J 2.1 Hz), 7.63 (1H, t, J 5.0 Hz), 7.60 (1H, dd, J 8.6, 2.1 Hz), 7.29 (1H, d, J 8.6 Hz), 3.47 (2H, s), 2.86 (2H, d, J 5.0 Hz), 2.76 (2H, s), 1.38 (6H, s), 1.01 (6H, s). LCMS (ES+) RT 2.55 minutes, 543/545 (M+H)+.
Example 2 (1.0 g, 2.6 mmol), EDC (362 mg, 2.9 mmol), HOBT (300 mg, 2.9 mmol), NMM (427 mg, 5.2 mmol) and O-(2-hydroxyethyl)hydroxylamine (163 mg, 2.6 mmol) in DMF (10 mL) and DCM (9 mL) were stirred for 48 h. The reaction mixture was poured onto water and extracted with DCM, the unreacted acid removed by filtration and the remaining organic phase washed with 1M aqueous HCl then dried over sodium sulphate and concentrated in vacuo. Chromatography (silica; ethyl acetate) yielded the title compound. δH (DMSO-d6) 11.22 (1H, br s), 8.82 (1H, br s), 7.93 (1H, t, J 4.6 Hz), 7.64 (1H, dd, J 10.7, 2.1 Hz), 7.48 (1H, d, J 8.5 Hz), 7.08 (1H, t, J 8.5 Hz), 4.71 (1H, br s), 3.81-3.74 (2H, m), 3.59-3.50 (2H, m), 2.86 (2H, d, J 5.0 Hz), 2.65 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.85 minutes, 534 (M+H)+.
From Example 76 (500 mg, 1.06 mmol), 2M trimethylaluminium in toluene (2.65 mL, 5.30 mmol) and (S)-(+)-(2,2-dimethyl-[1,3]dioxolan-4-yl)methylamine (700 μL, 5.30 mmol) in toluene (10 mL) by the method of Example 78. The reaction was worked up by pouring onto 2M aqueous HCl, extracted into ethyl acetate, the aqueous phase basified with sodium hydroxide and the resulting precipitate collected by filtration. After chromatography (silica; 5% methanol/DCM→10% methanol/DCM) the product was further purified by preparative HPLC yielding the title compound (32 mg, 5%). δH (d4-MeOH) 8.57 (1H, s), 7.82 (1H, d, J 2.0 Hz), 7.62 (1H, dd, J 8.6, 2.0 Hz), 3.88-3.84 (1H, br s), 3.62-3.55 (2H, m), 3.54-3.36 (2H, m), 3.05 (2H, s), 2.71 (2H, s), 1.09 (6H, s). Exchangeable protons were not observed. LCMS (ES+) RT 2.51 minutes, 563/565 (M+H)+.
Intermediate 40 (300 mg, 0.46 mol) in THF (10 mL) was treated with aqueous ammonia (1 mL) and the mixture stirred at r.t. for 0.5 h. The reaction mixture was diluted with water, extracted with DCM and the aqueous phase filtered, to yield, after washing with methanol and drying, the title compound (108 mg, 68%). δH (DMSO-d6) 9.89 (1H, s), 7.96 (1H, t, J 4.9 Hz), 7.83 (1H, d, J 2.0 Hz), 7.67 (1H, dd, J 8.6, 2.0 Hz), 7.47 (2H, br s), 7.27 (1H, d, J 8.6 Hz), 2.90 (2H, d, J 4.9 Hz), 2.82 (2H, s), 0.98 (6H, s). LCMS (ES+) RT 2.98 minutes, 490/492 (M+H)+.
Example 2 (673 mg, 1.42 mmol) and Intermediate 37 (300 mg, 1.7 mmol) in DMF (10 mL) were treated with CDI (300 mg, 1.84 mmol) and the reaction mixture stirred at r.t. for 18 h. The solvent was removed in vacuo, the residue partitioned between DCM and water, dried (sodium sulphate) and concentrated. The resulting oil was chromatographed (SiO2; DCM/ethyl acetate) then further purified by HPLC at pH 3 (HCO2H) to give the title compound (150 mg). δH (DMSO-d6) 8.25 (1H, s), 7.67 (1H, t, J=4.9 Hz), 7.75 (1H, dd, J 10.7, 1.9 Hz), 7.49 (1H, ddd, J 8.7, 2.1, 1.1 Hz), 7.25 (1H, t, J 8.9 Hz), 3.90 (2H, t, J 4.7 Hz), 2.99 (2H, s), 2.90 (2H, t, J 2.0 Hz), 2.81 (2H, d, J 5.1 Hz), 0.96 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT 2.14 minutes, 533 (M+H)+.
Example 2 (250 mg, 0.42 mmol) and CDI (75 mg, 0.46 mmol) were mixed in DMF (3 mL) and the reaction mixture stirred at ambient temperature for 15 minutes. Guanidine carbonate (76 mg, 0.42 mmol) and triethylamine (60 μL, 0.42 mmol) were added and the reaction mixture stirred at r.t. for 4 h then 100° C. for 1 h. The solvent was removed in vacuo and the product triturated with DCM then purified further by preparative HPLC to give the title compound. δH (DMSO-d6) 13.07 (1H, br s), 7.77 (1H, brt, J 4.8 Hz), 7.69 (1H, dd, J 10.5, 2.1 Hz), 7.60 (1H, ddd, J 8.5, 2.1, 1.1 Hz), 7.40 (1H, t, J 8.7 Hz), 3.13 (2H, s), 2.82 (2H, d, J 5.1 Hz), 0.97 (6H, s). Some exchangeable protons were not observed. LCMS (ES+) RT 2.62 minutes, 516 (M+H)+.
Intermediate 38 (190 mg, 0.29 mmol) was dissolved in a minimum of DCM and treated with 2M hydrogen chloride in diethyl ether (5 mL). After stirring at ambient temperature overnight the reaction mixture was concentrated in vacuo to yield the title compound (160 mg, quant.). δH (DMSO-d6) 8.96 (2H, br s), 8.38 (1H, d, J 6.8 Hz), 7.62 (1H, dd, J 10.5, 1.7 Hz), 7.46 (1H, d, J 8.7 Hz), 7.04 (1H, t, J 8.7 Hz), 4.42-4.32 (1H, m), 3.38-3.33 (1H, m), 3.25-3.16 (2H, m), 3.10 (2H, s), 3.04 (3H, s), 3.02-2.94 (1H, m), 2.63 (2H, s), 2.12-1.99 (1H, m), 1.82-1.69 (1H, m), 0.99 (6H, s).
Intermediate 31 (450 mg, 0.69 mmol) and 1,2-diamino-2-methylpropane (122 mg, 1.38 mmol) in DMF (5 mL) with triethylamine (78 mg, 0.69 mmol) were stirred at r.t. for 18 h. The solvent was removed in vacuo and the residue azeotroped with heptane then partitioned between ethyl acetate and 1M aqueous NaOH solution. The organic phase was separated, dried (sodium sulphate), concentrated and the residue subjected to column chromatography (silica; 0-10% MeOH in DCM) to yield the title compound (210 mg, 55%). δH (DMSO-d6) 9.92 (1H, br s), 7.47 (1H, dd, J 10.8, 1.8 Hz), 7.40 (1H, d, J 8.5 Hz), 7.16 (1H, t, J 8.7 Hz), 3.23 (2H, s), 3.03 (2H, s), 3.01 (3H, s), 2.93 (2H, s), 1.11 (6H, s), 0.98 (6H, s). Some exchangeable protons not observed. LCMS (ES+) RT 2.85 minutes, 559 (M+H)+.
Intermediate 5 (400 mg, 0.63 mmol) and 2,5-diaminopyridine (100 mg, 0.92 mmol) in DMF (5 mL) with triethylamine (88 μL, 0.63 mmol) were stirred at r.t. for 24 h. The solvent was removed in vacuo and the residue azeotroped with heptane then partitioned between 1M aqueous NaOH and ethyl acetate. The organic phase was separated, dried over sodium sulphate and the solvents removed in vacuo to give a crude residue which was purified by HPLC to yield the title compound. δH (DMSO-d6) 9.69 (1H, br s), 8.80 (1H, br s), 8.14 (0.4H, s, HCOOH), 8.00 (1H, br s), 7.96-7.86 (1H, br m), 7.59 (1H, dd, J 10.6, 1.9 Hz), 7.49 (1H, br s), 7.46 (1H, d, J 8.4 Hz), 7.10 (1H, t, J 8.8 Hz), 6.40 (1H, d, J 8.9 Hz), 5.74 (2H, s), 2.89 (2H, d, J 5.0 Hz), 2.73 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.14 minutes, 566 (M+H)+.
Intermediate 5 (400 mg, 0.63 mmol) and 2,6-diaminopyridine (100 mg, 0.92 mmol) in DMF (5 mL) with triethylamine (88 μL, 0.63 mmol) were stirred at r.t. for 24 h. The solvent was removed in vacuo and the residue azeotroped with heptane then partitioned between 1M aqueous NaOH and ethyl acetate. The organic phase was separated, dried over sodium sulphate and the solvents removed in vacuo to give a crude residue which was purified by HPLC to yield the title compound. δH (DMSO-d6) 7.91 (1H, br s), 7.58 (1H, d, J 10.7 Hz), 7.45 (1H, d, J 8.9 Hz), 7.35 (1H, t, J 7.8 Hz), 7.30-7.20 (1H, m), 7.13 (1H, t, J 8.7 Hz), 6.17 (1H, d, J 7.9 Hz), 5.75 (2H, br s), 2.86 (2H, d, J 4.8 Hz), 2.81-2.72 (2H, br m), 0.97 (6H, s). Some exchangeable protons not observed. LCMS (ES+) RT 2.76 minutes, 566 (M+H)+.
Intermediate 5 (300 mg, 0.63 mmol) and 3-(methylamino)propane-1,2-diol (80 mg, 0.76 mmol) in DMF (5 mL) were stirred at r.t. for 4 h. The solvent was removed in vacuo and the resulting oil azeotroped with heptane then partitioned between 1M aqueous NaOH and ethyl acetate. After drying over sodium sulphate and removal of the volatiles in vacuo the residue was purified by HPLC to yield the required product. δH (CDCl3) 7.49-7.40 (2H, m), 7.40-7.39 (2.5H, m), 7.12 (0.5H, br s), 6.06-5.97 (1H, m), 4.08-3.96 (1H, m), 3.96-3.79 (0.5H, m), 3.79-3.64 (1.5H, m), 3.64-3.52 (1.5H, m), 3.44-3.32 (2.5H, m), 3.17-3.10 (2H, m), 3.08 (3H, s), 3.04-2.92 (1H, m), 2.71-2.51 (3H, m), 1.10 (3H, s), 1.04 (3H, s). LCMS (ES+) RT 1.68 minutes, 562 (M+H)+.
Diisobutylaluminium hydride in toluene (1.0M, 2.3 mL, 2.3 mmol) was added to a solution of Example 1 (250 mg, 0.46 mmol) in DCM (25 mL) at 0° C. The mixture was allowed to warm to ambient temperature and stirred for 90 h. The crude reaction mixture was treated with saturated aqueous NH4Cl (125 mL) and an aqueous solution of Rochelle's salt (125 mL) and then extracted with DCM (3×125 mL). The combined organic extracts were washed with brine (200 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-25% ethyl acetate in DCM) to give the title compound as a white solid (146 mg, 69%). δH (DMSO-d6) 8.04 (1H, s), 7.93-7.89 (1H, m), 7.52 (1H, dd, J 10.9, 1.9 Hz), 7.32 (1H, d, J 8.4 Hz), 6.50-6.42 (1H, m), 3.29 (2H, s), 2.86 (2H, q, J 5.3 Hz), 1.85 (3H, s), 0.99 (6H, s). LCMS (ES+) RT 3.24 minutes, 445.2 (M+H)+.
Example 1 (30 mg, 0.05 mmol) and aqueous ammonia solution (2 mL) was dissolved in 1,4-dioxane (2 mL). The mixture was stirred at 120° C. for 90 minutes in the microwave. The crude reaction was concentrated in vacuo and partitioned between water (100 mL) and DCM. The aqueous phase was extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-35% ethyl acetate in DCM) to give the title compound as a beige solid (5 mg, 19%). δH (DMSO-d6) 9.89 (1H, s), 8.12-8.05 (1H, m), 7.72 (1H, d, J 10.7 Hz) 7.60 (1H, d, J 8.0 Hz), 7.42-7.32 (1H, m), 7.22 (2H, s), 2.88 (2H, s), 2.68 (2H, d, J 5.4 Hz), 0.95 (6H, s). LCMS (ES+) RT 2.93 minutes, 474 (M+H)+.
Diethanolamine (502 mg, 4.75 mmol) was added to a solution of Intermediate 5 (500 mg, 0.78 mmol) and triethylamine (0.3 ml, 2.15 mmol) in DCM (10 mL). The reaction mixture was stirred at room temperature for 72 h. Water (150 mL) was added to the crude reaction and the mixture extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-5% methanol in DCM) to give the title compound as a white solid (300 mg, 68%). δH (DMSO-d6) 8.30 (1H, s), 7.90-7.86 (1H, m), 7.59 (1H, dd, J 10.6, 1.8 Hz), 7.42 (1H, d, J 8.5 Hz), 7.04 (1H, t, J 8.7 Hz), 5.03 (1H, br s), 4.74 (1H, br s), 3.60-3.40 (8H, m), 3.28-3.27 (2H, m), 2.89 (2H, br s), 0.94 (6H, s). LCMS (ES+) RT 2.30 minutes, 562.2 (M+H)+.
From Intermediate 5 and 2-piperidinemethanol (350 mg, 2.5 mmol) by the method of Example 92 to give the crude product which was purified by chromatography (silica, 0-20% ethyl acetate in DCM) to give the title compound as a beige solid (114 mg, 42%). LCMS (ES+) RT 2.66 minutes, 572.2 (M+H)+.
From Intermediate 5 and (3R)-(+)-3-(tert-butoxycarbonylamino)pyrrolidine (300 mg, 1.6 mmol) by the method of Example 92 to give the crude product which was purified by chromatography (silica, 0-50% ethyl acetate in DCM) to give the title compound as a white solid (468 mg, 93%). δH (DMSO-d6, 100° C.) 8.14 (1H, s), 7.56-7.51 (2H, m), 7.44 (1H, d, J 8.5 Hz), 7.05-6.95 (1H, m), 6.60 (1H, s), 4.00-3.90 (1H, m), 3.60-3.40 (2H, m), 3.40-3.25 (1H, m), 3.20-3.10 (1H, m), 3.00-2.90 (4H, m), 2.05-1.95 (1H, m), 1.82-1.70 (1H, m), 1.40 (9H, s), 0.98 (6H, s). LCMS (ES+) RT 2.97 minutes, 643.2 (M+H)+.
From Intermediate 5 and (3S)-(−)-3-(tert-butoxycarbonylamino)pyrrolidine (300 mg, 1.6 mmol) by the method of Example 92 to give the crude product which was purified by chromatography (silica, 0-50% ethyl acetate in DCM) to give the title compound as a white solid (490 mg, 98%). δH (DMSO-d6, 100° C.) 8.14 (1H, s), 7.56-7.46 (2H, m), 7.44 (1H, d, J 8.5 Hz), 7.00 (1H, t, J 8.5 Hz), 6.60 (1H, s), 4.00-3.90 (1H, m), 3.58-3.40 (2H, m), 3.38-3.22 (1H, m), 3.16 (1H, dd, J 11.4, 5.2 Hz), 3.10-2.87 (4H, m), 2.05-1.95 (1H, m), 1.85-1.73 (1H, m), 1.40 (9H, s), 1.00 (6H, s). LCMS (ES+) RT 3.03 minutes, 643.2 (M+H)+.
From Intermediate 5 and (R)-pyrrolidin-2-ylmethylcarbamic acid tert-butyl ester (312 mg, 1.56 mmol) by the method of Example 92 to give the crude product which was purified by chromatography (silica, 20-50% ethyl acetate in DCM) to give the title compound as a white solid (482 mg, 94%). δH (DMSO-d6, 110° C.) 8.06 (1H, s), 7.52 (1H, dd, J 10.5, 1.9 Hz), 7.47 (1H, s), 7.42 (1H, d, J 8.5 Hz), 7.05-7.01 (1H, m), 6.30 (1H, s), 4.10-4.00 (1H, m), 3.40 (2H, s), 3.10-3.05 (1H, m), 3.00-2.95 (4H, m), 2.65-2.50 (1H, m), 1.92-1.65 (4H, m), 1.39 (9H, s), 1.01 (3H, s), 0.98 (3H, s). LCMS (ES+) RT 3.15 minutes, 657.2 (M+H)+.
From Intermediate 5 and (S)-pyrrolidin-2-ylmethylcarbamic acid tert-butyl ester (312 mg, 1.56 mmol) by the method of Example 92 to give the crude product which was purified by chromatography (silica, 20-50% ethyl acetate in DCM) to give the title compound as a white solid (504 mg, 98%). δH (DMSO-d6, 110° C.) 8.06 (1H, s), 7.52 (1H, dd, J 10.5, 1.8 Hz), 7.47 (1H, s), 7.42 (1H, d, J 8.5 Hz), 7.05-7.01 (1H, m), 6.30 (1H, s), 4.10-4.00 (1H, m), 3.34 (2H, br s), 3.10-3.05 (1H, m), 3.00-2.90 (4H, m), 2.65-2.50 (1H, m), 1.90-1.65 (4H, m), 1.40 (9H, s), 1.01 (3H, s), 0.98 (3H, s). LCMS (ES+) RT 3.14 minutes, 657.2 (M+H)+.
Example 94 (460 mg, 0.7 mmol) in ether (10 mL) was stirred at room temperature with 2N aqueous hydrochloric acid (4.8 ml, 9 mmol) for 65 h. Crude solids were filtered and washed with ether (2×5 mL) and dried to give the title compound as a yellow solid (368 mg, 91%). δH (DMSO-d6, 100° C.) 8.31 (3H, br s), 7.56 (1H, dd, J 10.4, 1.8 Hz), 7.50-7.40 (2H, m), 7.09 (1H, t, J 8.4 Hz), 3.85-3.73 (1H, m), 3.72-3.55 (2H, m), 3.52-3.40 (3H, m), 2.92 (2H, s), 2.55-2.45 (2H, m), 2.30-2.15 (1H, m), 2.10-1.98 (1H, m), 1.00 (6H, s). LCMS (ES+) RT 2.05 minutes, 543.0 (M+H)+.
Example 95 (506 mg, 0.78 mmol) in diethyl ether (10 mL) was stirred at room temperature with 2N aqueous hydrochloric acid (4.8 mL, 9 mmol) for 65 h. Crude solids were filtered and washed with ether (2×5 mL) and dried to give the title compound as a yellow solid (385 mg, 85%). δH (DMSO-d6, 110° C.) 8.35 (3H, v br s), 7.56 (1H, dd, J 10.4, 1.8 Hz), 7.50-7.40 (2H, m), 7.15-7.08 (1H, m), 3.80-3.73 (1H, m), 3.72-3.58 (2H, m), 3.57-3.33 (3H, m), 2.92 (2H, s), 2.55-2.50 (2H, m), 2.30-2.15 (1H, m), 2.15-1.98 (1H, m), 1.00 (6H, s). LCMS (ES+) RT 1.89 minutes, 543.0 (M+H)+.
Example 96 (460 mg, 0.70 mmol) in diethyl ether (10 mL) was stirred at room temperature with 2N aqueous hydrochloric acid (9.5 mL, 19 mmol) for 150 h. Crude solids were filtered and washed with ether (2×5 mL) and dried to give the title compound as an off-white solid (350 mg, 84%). δH (DMSO-d6, 130° C.) 8.07 (3H, br s), 7.55 (1H, dd, J 10.5, 1.7 Hz), 7.46 (1H, d, J 8.6 Hz), 7.41 (1H, br s), 7.09 (1H, t, J 8.6 Hz), 4.25 (1H, m), 3.40 (2H, m), 3.20-2.80 (6H, m), 2.64-2.45 (1H, m), 2.15-2.05 (1H, m), 1.92-1.70 (3H, m), 1.02 (3H, s), 0.99 (3H, s). LCMS (ES+) RT 2.02 minutes, 557.2 (M+H)+.
Example 97 (480 mg, 0.73 mmol) in diethyl ether (10 mL) was stirred at room temperature with 2N aqueous hydrochloric acid (9.5 mL, 19 mmol) for 150 h. Crude solids were filtered and washed with ether (2×5 mL) and dried to give the title compound as a white solid (380 mg, 87%). δH (DMSO-d6, 130° C.) 8.08 (3H, br s), 7.55 (1H, dd, J 10.5, 1.6 Hz), 7.46 (1H, d, J 8.5 Hz), 7.41 (1H, s), 7.12-7.08 (1H, m), 4.30-4.20 (1H, m), 3.50-3.38 (2H, m), 3.10-2.80 (6H, m), 2.62-2.46 (1H, m), 2.13-2.00 (1H, m), 1.92-1.70 (3H, m), 1.02 (3H, s), 0.99 (3H, s). LCMS (ES+) RT 2.01 minutes, 557.2 (M+H)+.
Paraformaldehyde (62 mg, 0.48 mmol) and sodium borohydride (35 mg, 0.55 mmol) was added to a solution of Example 60 (168 mg, 0.29 mmol) in methanol (10 mL). The reaction mixture was stirred at room temperature for 18 h. The pH was adjusted to 3 with the addition of aqueous 2N HCl, then basified with 10% aqueous NaOH solution and extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-15% methanol in DCM) to give the title compound as an off-white solid (148 mg, 92%). δH (DMSO-d6) 8.85 (1H, br s), 8.12-8.05 (1H, m), 8.00-7.90 (1H, m), 7.60 (1H, d, J 10.6 Hz), 7.43 (1H, d, J 8.3 Hz), 7.00 (1H, t, J=8.8 Hz), 4.30-4.12 (1H, m), 2.85 (2H, d, J 4.7 Hz), 2.75-2.60 (3H, m), 2.55-2.35 (2H, m), 2.30-2.15 (4H, m), 2.12-1.97 (1H, m), 1.70-1.50 (1H, m), 0.95 (6H, s). LCMS (ES+) RT 1.98 minutes, 557.4 (M+H)+.
tert-Butoxycarbonyl-DAP-OH (188 mg, 0.92 mmol) was added to a solution of Intermediate 5 (506 mg, 0.78 mmol) and triethylamine (0.13 mL, 0.92 mmol) in DCM (8 mL). The mixture was stirred at room temperature for 48 h. H2O (75 mL) was added followed by 2 mL of citric acid (sat aqueous solution) and extracted with DCM/EtOAc (75:25) (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was triturated with DCM (5 mL) to give the title compound as a white solid (225 mg, 74%). δH (DMSO-d6) 12.65 (1H, br s), 9.24 (1H, br s), 8.00-7.87 (2H, m), 7.65 (1H, dd, J 10.5, 1.8 Hz), 7.52 (1H, d, J 8.5 Hz), 7.22 (1H, t, J 8.7 Hz), 7.00 (1H, d, J 7.8 Hz), 4.20-4.08 (1H, m), 3.60-3.40 (2H, m), 2.87 (2H, d, J 4.8 Hz), 2.73 (2H, s), 1.36 (9H, s), 0.96 (6H, s). LCMS (ES+) RT 2.74 minutes, 659.2 (M)+.
Example 103 (205 mg, 0.31 mmol) in tetrahydrofuran (8 mL) was stirred at room temperature with 5N aqueous hydrochloric acid for 18 h. Crude solids were filtered and dried to give the title compound as an off-white solid (115 mg, 62%). δH (DMSO-d6) 13.86 (1H, br s), 9.36 (1H, br s), 8.39 (3H, br s), 8.20-8.10 (1H, m), 7.98-7.88 (1H, m), 7.67 (1H, dd, J 10.6, 1.5 Hz), 7.53 (1H, d, J 8.5 Hz), 7.30-7.20 (1H, m), 4.10-4.00 (1H, m), 3.80-3.60 (2H, m), 2.89 (2H, d, J 4.2 Hz), 2.78 (2H, s), 0.97 (6H, s). LCMS (ES+) RT 2.08 minutes, 561.1 (M+H)+.
(S)-Morpholin-3-ylmethanol (417 mg, 3.56 mmol) was added to a solution of Intermediate 5 (300 mg, 0.47 mmol) and triethylamine (0.33 mL, 2.38 mmol) in DCM (7 mL). The mixture was stirred at room temperature for 48 h. H2O (75 mL) was added and the mixture extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was triturated with DCM (5 mL) to give the title compound as a white solid (115 mg, 43%). LCMS (ES+) RT 2.42 minutes, 574.1 (M+H)+.
{N-(Ethoxycarbonylmethyl)-N-[3(R)-pyrrolidin-3-yl]amino}acetic acid ethyl ester (82 mg, 0.28 mmol) was added to a solution of Intermediate 5 (100 mg, 0.15 mmol) and triethylamine (0.15 mL, 1.08 mmol) in DCM (7 mL). The mixture was stirred at room temperature for 120 h. H2O (75 mL) was added and the pH was adjusted to 4 using aqueous citric acid solution. The mixture was extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-50% ethyl acetate in DCM) to give the title compound as a white solid (105 mg, 98%). δH (CDCl3) 7.50-7.38 (2H, m), 7.35-7.28 (2H, m), 6.43-6.30 (1H, m), 4.30-4.10 (4H, m), 3.90-3.20 (9H, m), 3.00 (2H, br s), 2.75-2.50 (2H, m), 2.14-2.05 (1H, m), 2.00-1.80 (1H, m), 1.40-1.20 (6H, m), 1.07 (3H, s), 1.04 (3H, s). LCMS (ES+) RT 2.75 minutes, 715.0 (M+H)+.
(R)-Piperidin-2-ylmethanol hydrochloride salt (237 mg, 1.56 mmol) was added to a solution of Intermediate 5 (500 mg, 0.78 mmol) and triethylamine (0.3 mL, 1.71 mmol) in DCM (8 mL). The mixture was stirred at room temperature for 18 h and later heated at 50° C. for 24 h. H2O (75 mL) was added and the pH was adjusted to 4 using aqueous citric acid solution. The mixture was extracted with DCM (3×100 ml). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-2% methanol in DCM) to give the title compound as a white solid (200 mg, 45%). 8H (DMSO-d6) 7.92 (1H, t, J 4.7 Hz), 7.80 (1H, dd, J 10.2, 1.9 Hz), 7.64 (1H, d, J 8.4 Hz), 7.42 (1H, t, J 8.5 Hz), 4.12 (2H, dq, J 10.8, 4.6 Hz), 3.40-3.12 (3H, m), 2.92 (2H, s), 2.87 (2H, d, J 5.1 Hz), 2.84-2.75 (1H, m), 2.55-2.45 (1H, m), 1.80-1.70 (1H, m), 1.70-1.58 (1H, m), 1.54-1.40 (1H, m), 1.40-1.10 (3H, m), 0.98 (6H, s). LCMS (ES+) RT 2.07 minutes, 572 (M+H)+.
(S)-Piperidin-2-ylmethanol hydrochloride salt (240 mg, 1.56 mmol) was added to a solution of Intermediate 5 (510 mg, 0.78 mmol) and triethylamine (0.3 mL, 1.71 mmol) in DCM (8 mL). The mixture was stirred at room temperature for 18 h and later heated at 50° C. for 24 h. H2O (75 mL) was added and the pH adjusted to 4 with aqueous citric acid solution. The mixture was extracted with DCM (3×100 mL) and the combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-2% methanol in DCM) to give the title compound as a white solid (200 mg, 45%). δH (DMSO-d6) 7.92-7.82 (1H, m), 7.76 (1H, d, J 10.0 Hz), 7.62 (1H, d, J 8.2 Hz), 7.39 (1H, t, J 8.5 Hz), 4.09 (2H, dq, J 10.7, 4.4 Hz), 3.40-3.12 (3H, m), 2.91 (2H, s), 2.86 (2H, d, J 5.0 Hz), 2.83-2.71 (1H, m), 2.50-2.40 (1H, m), 1.80-1.70 (1H, m), 1.68-1.58 (1H, m), 1.56-1.42 (1H, m), 1.40-1.10 (3H, m), 0.97 (6H, s). LCMS (ES+) RT 2.05 minutes, 572.2 (M+H)+.
(S)-4-N-tert-Butoxycarbonylpiperazine-2-carboxylic acid (336 mg, 1.45 mmol) was added to a solution of Intermediate 5 (300 mg, 0.46 mmol) and triethylamine (0.33 mL, 2.3 mmol) in DCM (8 mL). The mixture was stirred at room temperature for 18 h. Water (75 mL) was added and the mixture extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-10% methanol in DCM) to give the title compound as a white solid (285 mg, 78%). LCMS (ES+) RT 2.72 minutes, 687.1 (M+H)+.
(R)-4-N-tert-Butoxycarbonylpiperazine-2-carboxylic acid (346 mg, 1.50 mmol) was added to a solution of Intermediate 5 (300 mg, 0.46 mmol) and triethylamine (0.33 mL, 2.3 mmol) in DCM (8 mL). The mixture was stirred at room temperature for 18 h. Water (75 mL) was added and the mixture extracted with DCM (3×100 mL). The combined organic extracts were washed with brine (100 mL), dried (MgSO4), filtered and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-10% methanol in DCM) to give the title compound as a white solid (266 mg, 74%). LCMS (ES+) RT 2.72 minutes, 687.1 (M+H)+.
Example 109 (285 mg, 0.36 mmol) in tetrahydrofuran (8 mL) was stirred at room temperature with 5N aqueous hydrochloric acid for 18 h. Crude solids were filtered and dried to give the title compound as a yellow solid (199 mg, 80%). LCMS (ES+) RT 1.45 minutes, 587.1 (M+H)+.
Example 110 (266 mg, 0.34 mmol) in tetrahydrofuran (8 mL) was stirred at room temperature with 5N aqueous hydrochloric acid for 18 h. Crude solids were filtered and dried to give the title compound as a yellow solid (160 mg, 68%). LCMS (ES+) RT 1.60 minutes, 587.1 (M+H)+.
To Intermediate 41 (35 mg, 1 eq) was added a solution of the desired amine (0.8 eq) in THF/DMF (1:1, 2 mL). The mixture was stirred overnight at room temperature. The resin was then filtered and rinsed with a solution of THF/DMF (1:1). The organic solutions were combined and the volatiles removed in vacuo to give the desired products as crude solids. The compounds were purified by preparative HPLC and characterized by LCMS.
Examples 113 to 206 were all prepared by this method.
LCMS (ES+) RT 1.70 minutes, 545 (M+H)+.
LCMS (ES+) RT 1.77 minutes, 518 (M+H)+.
LCMS (ES+) RT 1.85 minutes, 541 (M+H)+.
LCMS (ES+) RT 1.65 minutes, 544 (M+H)+.
LCMS (ES+) RT 1.80 minutes, 532 (M+H)+.
LCMS (ES+) RT 1.88 minutes, 532 (M+H)+.
LCMS (ES+) RT 1.89 minutes, 559 (M+H)+.
LCMS (ES+) RT 1.65 minutes, 587 (M+H)+.
LCMS (ES+) RT 2.15 minutes, 574 (M+H)+.
LCMS (ES+) RT 2.03 minutes, 545 (M+H)+.
LCMS (ES+) RT 2.23 minutes, 586 (M+H)+.
LCMS (ES+) RT 2.39 minutes, 587 (M+H)+.
LCMS (ES+) RT 1.85 minutes, 585 (M+H)+.
LCMS (ES+) RT 2.05 minutes, 565 (M+H)+.
LCMS (ES+) RT 2.07 minutes, 579 (M+H)+.
LCMS (ES+) RT 2.14 minutes, 546 (M+H)+.
LCMS (ES+) RT 2.30 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.90 minutes, 565 (M+H)+.
LCMS (ES+) RT 2.09 minutes, 571 (M+H)+.
LCMS (ES+) RT 2.12 minutes, 558 (M+H)+.
LCMS (ES+) RT 1.75 minutes, 559 (M+H)+.
LCMS (ES+) RT 2.22 minutes, 558 (M+H)+.
LCMS (ES+) RT 2.04 minutes, 532 (M+H)+.
LCMS (ES+) RT 1.66 minutes, 671 (M+H)+.
LCMS (ES+) RT 2.35 minutes, 594 (M+H)+.
LCMS (ES+) RT 2.27 minutes, 574 (M+H)+.
LCMS (ES+) RT 2.28 minutes, 594 (M+H)+.
LCMS (ES+) RT 2.12 minutes, 594 (M+H)+.
LCMS (ES+) RT 1.82 minutes, 559 (M+H)+.
LCMS (ES+) RT 1.78 minutes, 559 (M+H)+.
LCMS (ES+) RT 1.58 minutes, 559 (M+H)+.
LCMS (ES+) RT 1.92 minutes, 558 (M+H)+.
LCMS (ES+) RT 2.28 minutes, 562 (M+H)+.
LCMS (ES+) RT 2.03 minutes, 594 (M+H)+.
LCMS (ES+) RT 1.69 minutes, 531 (M+H)+.
LCMS (ES+) RT 1.61 minutes, 557 (M+H)+.
LCMS (ES+) RT 1.70 minutes, 585 (M+H)+.
LCMS (ES+) RT 2.31 minutes, 592 (M+H)+.
LCMS (ES+) RT 1.61 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.92 minutes, 565 (M+H)+.
LCMS (ES+) RT 1.97 minutes, 579 (M+H)+.
LCMS (ES+) RT 1.74 minutes, 572 (M+H)+.
LCMS (ES+) RT 1.92 minutes, 571 (M+H)+.
LCMS (ES+) RT 1.99 minutes, 575 (M+H)+.
LCMS (ES+) RT 1.77 minutes, 586 (M+H)+.
LCMS (ES+) RT 1.77 minutes, 583 (M+H)+.
LCMS (ES+) RT 1.99 minutes, 604 (M+H)+.
LCMS (ES+) RT 2.39 minutes, 619 (M+H)+.
LCMS (ES+) RT 2.09 minutes, 586 (M+H)+.
LCMS (ES+) RT 1.80 minutes, 558 (M+H)+.
LCMS (ES+) RT 1.80 minutes, 568 (M+H)+.
LCMS (ES+) RT 1.69 minutes, 661 (M+H)+.
LCMS (ES+) RT 1.80 minutes, 569 (M+H)+.
LCMS (ES+) RT 1.45 minutes, 631 (M+H)+.
LCMS (ES+) RT 1.55 minutes, 638 (M+H)+.
LCMS (ES+) RT 1.62 minutes, 606 (M+H)+.
LCMS (ES+) RT 2.26 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.88 minutes, 592 (M+H)+.
LCMS (ES+) RT 1.84 minutes, 580 (M+H)+.
LCMS (ES+) RT 1.47 minutes, 532 (M+H)+.
LCMS (ES+) RT 2.13 minutes, 596 (M+H)+.
LCMS (ES+) RT 2.08 minutes, 572 (M+H)+.
LCMS (ES+) RT 2.41 minutes, 574 (M+H)+.
LCMS (ES+) RT 2.33 minutes, 572 (M+H)+.
LCMS (ES+) RT 1.65 minutes, 544 (M+H)+.
LCMS (ES+) RT 2.12 minutes, 586 (M+H)+.
LCMS (ES+) RT 1.81 minutes, 558 (M+H)+.
LCMS (ES+) RT 1.70 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.84 minutes, 572 (M+H)+.
LCMS (ES+) RT 2.03 minutes, 586 (M+H)+.
LCMS (ES+) RT 2.28 minutes, 593 (M+H)+.
LCMS (ES+) RT 2.07 minutes, 594 (M+H)+.
LCMS (ES+) RT 1.79 minutes, 586 (M+H)+.
LCMS (ES+) RT 1.70 minutes, 587 (M+H)+.
LCMS (ES+) RT 1.74 minutes, 605 (M+H)+.
LCMS (ES+) RT 1.76 minutes, 599 (M+H)+.
LCMS (ES+) RT 1.49 minutes, 586 (M+H)+.
LCMS (ES+) RT 2.08 minutes, 572 (M+H)+.
LCMS (ES+) RT 2.06 minutes, 573 (M+H)+.
LCMS (ES+) RT 2.12 minutes, 574 (M+H)+.
LCMS (ES+) RT 1.63 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.77 minutes, 545 (M+H)+.
LCMS (ES+) RT 2.05 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.95 minutes, 555 (M+H)+.
LCMS (ES+) RT 1.62 minutes, 589 (M+H)+.
LCMS (ES+) RT 1.47 minutes, 546 (M+H)+.
LCMS (ES+) RT 1.50 minutes, 586 (M+H)+.
LCMS (ES+) RT 1.46 minutes, 572 (M+H)+.
LCMS (ES+) RT 1.80 minutes, 599 (M+H)+.
LCMS (ES+) RT 2.20 minutes, 585 (M+H)+.
LCMS (ES+) RT 1.89 minutes, 601 (M+H)+.
LCMS (ES+) RT 1.70 minutes, 558 (M+H)+.
LCMS (ES+) RT 1.70 minutes, 532 (M+H)+.
LCMS (ES+) RT 2.35 minutes, 560 (M+H)+.
Intermediate 43 (1.4 g, 2.6 mmol) and ammonium acetate (2.0 g, 26 mmol) were dissolved in MeCN (25 mL) and heated at reflux for 4 h. The reaction mixture was cooled, 10% aqueous NaOH was added and the reaction mixture was extracted with DCM (3×100 mL). The combined organics were further washed with NaHCO3 and brine and then the organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude residue was triturated (1:1) with MeCN and isopropyl ether, and the precipitate was filtered off and dried to give the title compound as a cream solid (550 mg, 42%). δH (DMSO-d6) 10.04 (1H, br s), 8.85 (1H, br s), 7.87 (1H, dd, J 10.0, 1.8 Hz), 7.71 (1H, dd, J 8.4, 1.1 Hz), 7.41 (1H, t, J 8.5 Hz), 4.36 (2H, q, J 7.1 Hz), 2.91 (4H, d, J 4.2 Hz), 1.34 (3H, t, J 7.1 Hz), 1.02 (6H, s). One exchangeable proton was not observed. LCMS (ES+) RT 2.46 minutes, 502 (M+H)+.
Trimethylaluminium (1.72 mL, 3.45 mmol) was added to a solution of 1,2-diamino-2-methylpropane (303 mg, 3.45 mmol) in THF (10 mL) at 0° C. and stirred for 30 minutes. Example 207 was added to the mixture and then heated to 100° C. for 4 h. Aqueous NaOH (10%) was added to the reaction mixture and the aqueous phase extracted with EtOAc (3×100 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by trituration with MeCN and filtered off to give the title compound as a yellow solid (149 mg, 40%). δH (DMSO-d6) 10.52 (1H, t, J 5.6 Hz), 7.49 (1H, dd, J 10.3, 1.9 Hz), 7.41 (1H, d, J 8.5 Hz), 7.12 (1H, t, J 8.7 Hz), 3.23 (2H, s), 3.12 (2H, d, J 5.7 Hz), 2.80 (2H, s), 0.98 (12H, s). LCMS (ES+) RT 2.23 minutes, 544 (M+H)+.
2-Fluoro-4-iodoaniline (35 mg, 0.15 mmol) was dissolved in THF and the solution cooled to 0° C. Sodium hexamethyldisilazide (0.6M solution in THF, 0.27 ml) was added dropwise to the solution and the reaction was stirred for 10 min. Intermediate 48 (36 mg, 0.135 mmol) was then added and the reaction stirred for a further 3 h. The solvent was then removed under reduced pressure and purified by preparative HPLC to yield the title compound (3 mg, 5%). δH (d6-DMSO) 10.79 (1H, s), 7.82-7.75 (1H, m), 7.64-7.58 (1H, m), 7.25 (1H, t, J 8.9 Hz), 2.65 (2H, s), 2.34 (2H, s), 1.06 (6H, s). LCMS (ES+) RT (pH 10) 2.63 minutes, 441 (M+H)+.
Hydroxylamine (0.5 mL, 50% solution in water) was added to a solution of Example 209 (83 mg, 0.19 mmol) in THF (5 mL) and heated to reflux for 18 h. The reaction was cooled and brine (50 mL) was added to the residue and the mixture extracted with EtOAc (3×50 mL). The combined organic extracts were dried (Na2SO4) and concentrated in vacuo. The crude product was purified by chromatography (silica, 0-30% EtOAc in DCM) to give the title compound as a cream solid (13 mg, 23%). δH (DMSO-d6) 11.33 (1H, br s), 7.74 (1H, d, J 10.4 Hz), 7.62 (1H, d, J 8.4 Hz), 7.44-7.39 (3H, m), 2.93 (2H, s), 2.35 (2H, s), 1.05 (6H, s). LCMS (ES+) RT 2.94 minutes, 459 (M+H)+.
To a stirred solution of 2-fluoro-4-iodoaniline (294 mg, 1.24 mmol) in anhydrous THF (5 mL) at 0° C. was added a solution of lithium hexamethyldisilazide (1.12 mL, 1.24 mmol, 1.06M in THF). The reaction mixture was allowed to stir at 0° C. for one hour. A solution of Intermediate 57 (258 mg, 0.52 mmol) in anhydrous THF (5 mL) was added to the reaction mixture and stirred at ambient temperature for 18 hours. The reaction was quenched by the addition of saturated brine (100 mL) and extracted with EtOAc (3×100 mL), then the combined organic fractions were dried (Na2SO4), filtered and the volatiles removed in vacuo. The crude residue was dissolved in DCM (5 mL) and 4M HCl in 1,4-dioxane (1 mL) added. The mixture was stirred at ambient temperature for 3 hours before being neutralized to pH 7.4 by the addition of 10% aqueous sodium hydroxide solution. The aqueous phase was extracted with DCM (3×100 mL) and the combined organic fractions were dried (Na2SO4), filtered and the volatiles removed in vacuo. The crude residue was purified by column chromatography (SiO2, 0-30% EtOAc in DCM) to give the title compound as a pale pink solid (30 mg, 10%). δH (DMSO-d6) 10.89 (1H, brs), 7.48-7.40 (2H, m), 7.27 (1H, t, J 8.6 Hz), 5.29 (1H, s), 4.24 (2H, q, J 7.1 Hz), 3.13 (2H, s), 1.31 (6H, s), 1.30 (3H, t, J 7.1 Hz). LCMS (ES+) RT 3.60 minutes, 489 (M+H)+.
When ranges are used herein, for example, for biological activity, such as binding data, chemical properties, such as chemical formulae, or dosage ranges, all combinations and subcombinations of ranges and specific embodiments therein are intended to be included.
The disclosures of each patent, patent application and publication cited or described in this document are hereby incorporated herein by reference, in their entirety.
Those skilled in the art will appreciate that numerous changes and modifications can be made to the preferred embodiments of the invention and that such changes and modifications can be made without departing from the spirit of the invention. It is, therefore, intended that the appended claims cover all such equivalent variations as fall within the true spirit and scope of the invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 0616214.3 | Aug 2006 | GB | national |
The present application is a continuation of International Application No.: PCT/GB2007/003114, filed Aug. 15, 2007, which claims priority under 119(a-d) to Great Britain Application No. GB 0616214.3, filed Aug. 15, 2006. Each of these applications is hereby incorporated herein by reference in their entireties.
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/GB2007/003114 | Aug 2007 | US |
| Child | 12371124 | US |
