Claims
- 1. In a method for the determination of gamma-glutamyl transferase wherein the transferase liberates a colored product from a substrate in the presence of an amino acid or peptide acceptor, and the rate of release of the colored product is measured optically as a measure of the transferase activity, the improvement comprising using, as the substrate, the compound of the formula ##STR8## wherein X is C.sub.1 -C.sub.7 alkyl, --(CH.sub.2).sub.m --COOH or --O--(CH.sub.2).sub.n --COOH wherein m is 0, 1, 2, 3 or 4 and n is 1, 2, 3 or 4;
- R is p-nitrophenyl; p-nitrophenyl substituted one or more times by halogen, cyano, C.sub.1 -C.sub.7 alkoxy, hydroxyl, C.sub.1 -C.sub.7 alkyl, carboxy-C.sub.1 -C.sub.7 -alkyl, amino, C.sub.1 -C.sub.7 -alkyl-amino or di-C.sub.1 -C.sub.7 -alkyl-amino, the substituents being the same or different; or R is a thiophene, a thiophene substituted one or more times by cyano, nitro, C.sub.1 -C.sub.7 alkyl or carboxy-C.sub.1 -C.sub.7 -alkyl, the substituents being the same or different; or R is a thiazole; a thiazole substituted one or more times by cyano, carboxyl or C.sub.1 -C.sub.7 alkyl, the substituents being the same or different; or R is a benzothiazole, a benzothiazole substituted one or more times by C.sub.1 -C.sub.7 -alkoxy; or R is a benzoisothiazole; a benzoisothiazole which is substituted one or more times by nitro; or R is an N-C.sub.1 -C.sub.7 alkyl-thiazole; a 1,3,5-thiodiazole or a 1,3,5-thiodazole which is substituted one or more times by C.sub.1 -C.sub.7 alkylthio; or
- an alkali metal, alkaline earth metal or ammonium salt thereof.
- 2. The method of claim 1 wherein each alkyl or alkoxy is selected from the group consisting of methyl, ethyl, methoxy and ethoxy.
- 3. The method of claim 1 wherein the alkali metal salts are lithium, sodium, potassium, rubidium or caesium salts and the alkaline earth metal salts are magnesium, calcium, strontium or barium salts and the ammonium salts contain ammonium ions which are unsubstituted or are substituted 1 to 4 times by C.sub.1 -C.sub.7 alkyl or C.sub.6 -C.sub.10 -ara-C.sub.1 -C.sub.7 alkyl.
- 4. The method of claim 1 wherein the salts are lithium, sodium, potassium, magnesium, calcium, unsubstituted ammonium or ammonium ions substituted with C.sub.1 -C.sub.7 alkyl or benzyl.
- 5. In a diagnostic agent for the determination of the activity of the enzyme gamma-glutamyl transferase containing at least one chromogenic substrate, an amino acid or a peptide acceptor and a buffer system, as well as an adjuvant, the improvement comprising using as said chromogenic substrate the compound of claim 1.
- 6. In a method for the determination of gamma-glutamyl transferase wherein the transferase liberates a colored product from a substrate in the present of an amino acid or peptide acceptor, and the rate of release of the colored product is measured optically as a measure of the transferase activity, the improvement comprising using, as the substrate at least one of the compounds selected from the group consisting of
- 2-amino-5-[[4-(2-cyano-4-nitrophenyl)-azo-3-(2-carboxyethyl)phenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[4-(2-cyano-4-nitrophenyl)-azo-3-(2-carboxyethoxy)phenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-(carboxy-4-(4-nitrophenyl)-azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5[[4-(2,5-dichloro-4-nitrophenyl)-azo-3-(carboxymethoxy)-phenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-(2-carboxyethyl)-4-(2,4-dicyano-3-methyl-5-thienyl)-azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-carboxymethoxy-4-(2,4-dicyano-3-methyl-5-thienyl-azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[4-(5-carboxy-4-methyl-2-thiazolyl-3-methylazophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-(2-carboxyethyl)-4-(5-cyano-4-methyl-2-thiazolyl)-azophenyl]amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-(2-carboxyethyl)-4-(2-thiazolyl)azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-(2-carboxylatoethyl)-4-(3-methylthiazolium-2-yl)-azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-carboxymethoxy-4-(6-nitro-2,1-benzisothiazol-3-yl)-azophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[4-(2-butylthio-1,3,4-thiadiazol-5-yl)-3-carboxymethoxyazophenyl]-amino]-5-oxopentanoic acid,
- 2-amino-5-[[3-carboxymethoxy-4-(6-ethoxy-2-benzothiazolyl)azophenyl]-amino]-5-oxopentanoic acid and the alkali metal, alkaline earth metal or ammonium salts thereof.
- 7. In a diagnostic agent for the determination of the activity of the enzyme gamma-glutamyl transferase containing at least one chromogenic substrate, an amino acid or a peptide acceptor and a buffer system, as well as, an adjuvant the improvement comprising using as said chromogenic substrate at least one of the compound of claim 6.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3602443 |
Jan 1986 |
DEX |
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Parent Case Info
This application is a divisional application of U.S. Ser. No. 007,077, filed 01/27/87 now U.S. Pat. No. 4,769,447.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4560650 |
Bauer et al. |
Dec 1985 |
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Non-Patent Literature Citations (1)
Entry |
Walter, Methods of Enzymatic Analysis, vol. V, Bergmeyer (Ed.), Verlay Chemie, Weinheim, 270-277 (1984), 3rd Edition. |
Divisions (1)
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Number |
Date |
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Parent |
7077 |
Jan 1987 |
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