Claims
- 1. A DNA comprising a DNA encoding the following amino acid sequence: AGGGYWHTSGREILDANNVPVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIR LPYSDDILKPGTMPNSINFYQMNQDLQGLTSLQVMDKIVAYAGQIGLRIILDRHRPDCSGQS ALWYTSSVSEATWISDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAG NAVLSVNPNLLIFVEGVQSYNGDSYWWGGNLQGAGQYPVVLNVPNRLVYSAHDYATSVYPQT WFSDPTFPNNMPGIWNKNWGYLFNQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTAQYG ADSFQWTFWSWNPDSGDTGGILKDDWQTVDTVKDGYLAPIKSSIFDPVGASASPSSQPSPSV SPSPSPSPSASRTPTPTPTPTASPTPTLTPTATPTPTASPTPSPTAASGARCTASYQVNSDW GNGFTVTVAVTNSGSVATKTWTVSWTFGGNQTITNSWNAAVTQNGQSVTARNMSYNNVIQPG QNTTFGFQASYTGSNAAPTVACAAS (SEQ ID NO:3).
- 2. The DNA according to claim 1 further comprising the following sequence attached to an amino terminal end:
- MLRVGVVVAVLALVAALANLAVPRPARA, (SEQ ID NO:4).
- 3. The DNA according to claim 2 further comprising the following sequence attached to an amino terminal end:
- VPRALRRVPGSRV, SEQ ID NO:5.
- 4. The DNA according to claim 1 comprising the following sequence:
- __________________________________________________________________________GGATCCACGT TGTACAAGGT CACCTGTCCG TCGTTCTGGT AGAGCGGCGG 50GATGGTCACC CGCACGATCT CTCCTTTGTT GATGTCGACG GTCACGTGGT 100TACGGTTTGC CTCGGCCGCG ATTTTCGCGC TCGGGCTTGC TCCGGCTGTC 150GGGTTCGGTT TGGCGTGGTG TGCGGAGCAC GCCGAGGCGA TCCCAATGAG 200GGCAAGGGCA AGAGCGGAGC CGATGGCACG TCGGGTGGCC GATGGGGTAC 250GCCGATGGGG CGTGGCGTCC CCGCCGCGGA CAGAACCGGA TGCGGAATAG 300GTCACGGTGC GACATGTTGC CGTACCGCGG ACCCGGATGA CAAGGGTGGG 350TGCGCGGGTC GCCTGTGAGC TGCCGGCTGG CGTCTGGATC ATGGGAACGA 400TCCCACCATT CCCCGCAATC GACGCGATCG GGAGCAGGGC GGCGCGAGCC 450GGACCGTGTG GTCGAGCCGG ACGATTCGCC CATACGGTGC TGCAATGCCC 500AGCGCCATGT TGTCAATCCG CCAAATGCAG CAATGCACAC ATGGACAGGG 550ATTGTGACTC TGAGTAATGA TTGGATTGCC TTCTTGCCGC CTACGCGTTA 600CGCAGAGTAG GCGACTGTAT GCGGTAGGTT GGCGCTCCAG CCGTGGGCTG 650GACATGCCTG CTGCGAACTC TTGACACGTC TGGTTGAACG CGCAATACTC 700CCAACACCGA TGGGATCGTT CCCATAAGTT TCCGTCTCAC AACAGAATCG 750GTGCGCCCTC ATGATCAACG TGAAAGGAGT ACGGGGGAGA ACAGACGGGG 800GAGAAACCAA CGGGGGATTG GCGGTGCCGC GCGCATTGCG GCGAGTGCCT 850GGCTCGCGGG TGATGCTGCG GGTCGGCGTC GTCGTCGCGG TGCTGGCATT 900GGTTGCCGCA CTCGCCAACC TAGCCGTGCC GCGGCCGGCT CGCGCCGCGG 950GCGGCGGCTA TTGGCACACG AGCGGCCGGG AGATCCTGGA CGCGAACAAC 1000GTGCCGGTAC GGATCGCCGG CATCAACTGG TTTGGGTTCG AAACCTGCAA 1050TTACGTCGTG CACGGTCTCT GGTCACGCGA CTACCGCAGC ATGCTCGACC 1100AGATAAAGTC GCTCGGCTAC AACACAATCC GGCTGCCGTA CTCTGACGAC 1150ATTCTCAAGC CGGGCACCAT GCCGAACAGC ATCAATTTTT ACCAGATGAA 1200TCAGGACCTG CAGGGTCTGA CGTCCTTGCA GGTCATGGAC AAAATCGTCG 1250CGTACGCCGG TCAGATCGGC CTGCGCATCA TTCTTGACCG CCACCGACCG 1300GATTGCAGCG GGCAGTCGGC GCTGTGGTAC ACGAGCAGCG TCTCGGAGGC 1350TACGTGGATT TCCGACCTGC AAGCGCTGGC GCAGCGCTAC AAGGGAAACC 1400CGACGGTCGT CGGCTTTGAC TTGCACAACG AGCCGCATGA CCCGGCCTGC 1450TGGGGCTGCG GCGATCCGAG CATCGACTGG CGATTGGCCG CCGAGCGGGC 1500CGGAAACGCC GTGCTCTCGG TGAATCCGAA CCTGCTCATT TTCGTCGAAG 1550GTGTGCAGAG CTACAACGGA GACTCCTACT GGTGGGGCGG CAACCTGCAA 1600GGAGCCGGCC AGTACCCGGT CGTGCTGAAC GTGCCGAACC GCCTGGTGTA 1650CTCGGCGCAC GACTACGCGA CGAGCGTCTA CCCGCAGACG TGGTTCAGCG 1700ATCCGACCTT CCCCAACAAC ATGCCCGGCA TCTGGAACAA GAACTGGGGA 1750TACCTCTTCA ATCAGAACAT TGCACCGGTA TGGCTGGGCG AATTCGGTAC 1800GACACTGCAA TCCACGACCG ACCAGACGTG GCTGAAGACG CTCGTCCAGT 1850ACCTACGGCC GACCGCGCAA TACGGTGCGG ACAGCTTCCA GTGGACCTTC 1900TGGTCCTGGA ACCCCGATTC CGGCGACACA GGAGGAATTC TCAAGGATGA 1950CTGGCAGACG GTCGACACAG TAAAAGACGG CTATCTCGCG CCGATCAAGT 2000CGTCGATTTT CGATCCTGTC GGCGCGTCTG CATCGCCTAG CAGTCAACCG 2050TCCCCGTCGG TGTCGCCGTC TCCGTCGCCG AGCCCGTCGG CGAGTCGGAC 2100GCCGACGCCT ACTCCGACGC CGACAGCCAG CCCGACGCCA ACGCTGACCC 2150CTACTGCTAC GCCCACGCCC ACGGCAAGCC CGACGCCGTC ACCGACGGCA 2200GCCTCCGGAG CCCGCTGCAC CGCGAGTTAC CAGGTCAACA GCGATTGGGG 2250CAATGGCTTC ACGGTAACGG TGGCCGTGAC AAATTCCGGA TCCGTCGCGA 2300CCAAGACATG GACGGTCAGT TGGACATTCG GCGGAAATCA GACGATTACC 2350AATTCGTGGA ATGCAGCGGT CACGCAGAAC GGTCAGTCGG TAACGGCTCG 2400GAATATGAGT TATAACAACG TGATTCAGCC TGGTCAGAAC ACCACGTTCG 2450GATTCCAGGC GAGCTATACC GGAAGCAACG CGGCACCGAC AGTCGCCTGC 2500GCAGCAAGTT AATACGTCGG GGAGCCGACG GGAGGGTCCG GACCGTCGGT 2550TCCCCGGCTT CCACCTATGG AGCGAACCCA ACAATCCGGA CGGAACTGCA 2600GGTACCAGAG AGGAACGACA CGAATGCCCG CCATCTCAAA ACGGCTGCGA 2650GCCGGCGTCC TCGCCGGGGC GGTGAGCATC GCAGCCTCCA TCGTGCCGCT 2700GGCGATGCAG CATCCTGCCA TCGCCGCGAC GCACGTCGAC AATCCCTATG 2750CGGGAGCGAC CTTCTTCGTC AACCCGTACT GGGCGCAAGA AGTACAGAGC 2800GAACGGCGAA CCAGACCAAT GCCACTCTCG CAGCGAAAAT GCGCGTCGTT 2850TCCACATATT CGACGGCCGT CTGGATGGAC CGCATCGCTG CGATCAACGG 2900CGTCAACGGC GGACCCGGCT TGACGACATA TCTGGACGCC GCCCTCTCCC 2950AGCAGCAGGG AACCACCCCT GAAGTCATTG AGATTGTCAT CTACGATCTG 3000CCGG3004 SEQ ID NO: 6.__________________________________________________________________________
- 5. A vector comprising the DNA according to claim 1 and a vector sequence encoding either an origin of replication or an integration site for a host genome.
- 6. A vector according to claim 5 further comprising DNA encoding a signal sequence operably linked thereto.
- 7. A vector according to claim 5 further comprising exogenous regulatory sequences capable of causing expression of said DNA in a suitable host.
- 8. A recombinant microorganism containing the vector according to claim 5.
- 9. A recombinant microorganism containing the vector according to claim 6.
- 10. A recombinant microorganism containing the vector according to claim 7.
- 11. A recombinant microorganism according to claim 5 wherein a genus of said microorganism is selected from the group consisting of Saccharomyces, Streptomyces, Bacillus, Zymomonas and Escherichia.
- 12. A method for producing an endoglucanase comprising culturing the recombinant microorganism according to claim 8 in a vessel under culture conditions sufficient to express said DNA and recovering said endoglucanase therefrom.
- 13. The method according to claim 12, further comprising separating the recombinant microorganism from microbial medium and recovering said endoglucanase from the medium.
- 14. A method for producing an endoglucanase according to claim 12, further comprising effectively increasing the permeability of a membrane of the recombinant microorganism to permit release of said endoglucanase.
- 15. A DNA comprising at least one domain but not all of the domains of the Acidothermus cellulolyticus E1 endoglucanase.
- 16. The DNA according to claim 15 further comprising at least one domain from a cellulase gene other than E1 endoglucanase.
- 17. The DNA according to claim 16 wherein the DNA encodes a protein having a cellulase activity.
- 18. The DNA according to claim 17 wherein the cellulase activity is an endoglucanase activity.
Parent Case Info
This application is a continuation-in-part of Ser. No. 08/125,115 filed Sep. 21, 1993, now U.S. Pat. No. 5,366,884, which is a continuation-in-part of 07/826,089 filed Jan. 27, 1992, now U.S. Pat. No. 5,275,944, which was a continuation-in-part of Ser. No. 412,434 filed Sep. 26, 1989 now U.S. Pat. No. 5,110,735.
Government Interests
The United States Government has rights in this invention under Contract No. DE-AC02-83CH10093 between the United States Department of Energy and the National Renewable Energy Laboratory, a Division of the Midwest Research Institute.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5110735 |
Tucker et al. |
May 1992 |
|
5275944 |
Himmel et al. |
Jan 1944 |
|
5366884 |
Adney et al. |
Nov 1994 |
|
Non-Patent Literature Citations (2)
Entry |
Mohagheghi et al., Int. J. System. Bacteriol., 36:435-443 (1986). |
Lejeune et al., Biosynthesis and Biodegradation of Cellulose, C. Haigler and P. J. Weimer, Ed., Marcel-Dekker, NY 1991, pp. 623-672. |
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
125115 |
Sep 1993 |
|
Parent |
826089 |
Jan 1992 |
|
Parent |
412434 |
Sep 1989 |
|