Claims
- 1. A method of producing or enhancing production of a carotenoid in a host cell, relative to an untransformed host cell, the method comprising inserting into the host cell a vector comprising a heterologous nucleic acid sequence which encodes for a protein having ∈-cyclase enzyme activity, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence to produce the protein.
- 2. The method of claim 1, wherein the heterologous nucleic acid sequence has a sequence which encodes an amino acid sequence which is at least 85% identical to SEQ ID NOS:2, 23 or 25-27.
- 3. The method of claim 1, wherein the heterologous nucleic acid sequence has a sequence which encodes the amino acid sequence of SEQ ID NOS:2, 23 or 25-27.
- 4. The method of claim 1, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell, a yeast cell and a plant cell.
- 5. The method of claim 1, wherein the host cell is a photosynthetic cell.
- 6. A method of modifying the production of carotenoids in a host cell, the method comprising inserting into the host cell a vector comprising a heterologous nucleic acid sequence which produces an RNA and/or encodes for a protein which modifies ∈-cyclase enzyme activity, relative to an untransformed host cell, wherein the heterologous nucleic acid sequence is operably linked to a promoter; and expressing the heterologous nucleic acid sequence in the host cell to modify the production of the carotenoids in the host cell, relative to the untransformed host cell.
- 7. The method of claim 6, wherein the heterologous nucleic acid sequence has a sequence which encodes an amino acid sequence which is at least 85% identical to SEQ ID NOS:2, 23 or 25-27.
- 8. The method of claim 6, wherein the heterologous nucleic acid sequence has a sequence which encodes the amino acid sequence of SEQ ID NOS:2, 23 or 25-27.
- 9. The method of claim 6, wherein the host cell is selected from the group consisting of a bacterial cell, an algal cell, a yeast cell, and a plant cell.
- 10. The method of claim 6, wherein the host cell is a photosynthetic cell.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. application Ser. Nos. 09/088,724, filed Jun. 2, 1998, 09/088,725, filed Jun. 2, 1998, and 08/937,155, filed Sep. 25, 1997, U.S. application Ser. No. 08/937,155 being a divisional of U.S. application Ser. No. 08/624,125, filed Mar. 29, 1996, now U.S. Pat. No. 5,744,341.
Divisions (1)
|
Number |
Date |
Country |
Parent |
08624125 |
Mar 1996 |
US |
Child |
08937155 |
Sep 1997 |
US |
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
09088725 |
Jun 1998 |
US |
Child |
09323998 |
Jun 1999 |
US |
Parent |
09088724 |
Jun 1998 |
US |
Child |
09088725 |
Jun 1998 |
US |
Parent |
08937155 |
Sep 1997 |
US |
Child |
09088724 |
Jun 1998 |
US |