Genetic Engineering of Acidity in Tomato Fruit I Characterization of Genes for NADP-Malic Enzyme in Tomato Fruit

Information

• NSF Award
• 9362031

Owner

• DNA Plant Technology Corporation

• Award Id

  9362031

• Award Effective Date

  2/15/1994 - 30 years ago

• Award Expiration Date

  1/31/1995 - 29 years ago

• Award Amount

  $ 73,155.00

• Award Instrument

  Standard Grant

9362031 Dunsmuir Flavor in tomato fruit is associated with high levels of both sugars and acids. It is known that the level of malic acid in the fruit declines by 50% with fruit ripening and that this reduction is thought to be mediated by a cytosolic NADP dependent malic enzyme (NADP-ME). The tomato NADP-ME genes which are expressed in the pericarp during ripening by using a heterologous probe for NADP-ME from maize will be isolated and characterized. Once isolated, the tomato genes will be used in genetic engineering experiments so that the induction of NADP-ME which occurs during ripening would be reduced or inhibited and higher malic acid levels would persist within the ripe fruit. In parallel higher levels of the same enzyme in the fruit will be engineered in order to gain insight into mechanisms which regulate acid levels in the fruit. %%% The anticipated outcome of this research would be the ability to directly engineer the acid levels in fruit which could significantly affect the flavor of tomatoes in the treatment of processing tomatoes. ***

• Program Officer

  Kesh S. Narayanan

• Min Amd Letter Date

  2/19/1994 - 30 years ago

• Max Amd Letter Date

  10/27/1994 - 30 years ago

• ARRA Amount

• Name

  DNA Plant Technologies, Inc.

• City

  Oakland

• State

  CA

• Country

  United States

• Address

  6701 San Pablo Avenue

• Postal Code

  946081244

• Phone Number

  5105472395

• First Name

  Pamela

• Last Name

  Dunsmuir

• Start Date

  2/15/1994 12:00:00 AM

• Name

  Agriculture

• Code

  201000