GENETIC METHOD TO ANALYZE BACS AND CONSTRUCT CONTIG MAPS

Information

• Research Project
• 2828661

• ApplicationId

  2828661

• Core Project Number

  R43HG001967

• Full Project Number

  1R43HG001967-01

• Serial Number

  1967

• FOA Number

• Sub Project Id

• Project Start Date

  3/1/1999 - 25 years ago

• Project End Date

  8/31/1999 - 25 years ago

• Program Officer Name

  FELSENFELD, ADAM

• Budget Start Date

  3/1/1999 - 25 years ago

• Budget End Date

  8/31/1999 - 25 years ago

• Fiscal Year

  1999

• Support Year

  1

• Suffix

• Award Notice Date

  2/20/1999 - 25 years ago

Organizations

• STRATAGENE

The goal of this proposal is to develop a genetic method to analyze BAC DNA clones and create a contig map of a BAC library. This method avoids the requirement of redundant sequencing in analyzing a BAC clone and provides a method for contig construction that does not involve hybridization or multiplex PCR analysis. PROPOSED COMMERCIAL APPLICATIONS: The system of transposons, plasmids, and E. coli host strains used would provide the research lab with the tools necessary to study individual sections of BAC clones and construct contig maps that do not require expensive instrumentation or large quantities of biochemical reagents.

IC Name

• Activity

  R43

• Administering IC

  HG

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  172

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  ZRG5

• Study Section Name

• Organization Name

  STRATAGENE

• Organization Department

• Organization DUNS

• Organization City

  SAN DIEGO

• Organization State

  CA

• Organization Country

  UNITED STATES

• Organization Zip Code

  92037

• Organization District

  UNITED STATES