Claims
- 1. A method for producing a germanium-enriched dried yeast product comprising the steps of:
- contacting live yeast cells with an aqueous solution containing a germanium compound selected from the group consisting of carboxyethyl germanium sesquioxide and dicarboxygermanium sesquioxide;
- incubating said yeast cells in growth medium;
- isolating said yeast cells;
- washing said isolated yeast cells to remove extracellular germanium; and
- drying yeast cells containing intracellular germanium.
- 2. The method of claim 1 wherein said contacting step lasts from about 5 minutes to about 60 minutes.
- 3. The method according to claim 1 wherein said aqueous solution comprises the germanium compound diluted in purified water.
- 4. The method of claim 1 wherein the pH of the aqueous solution ranges from between about pH 4 to about pH 7.
- 5. The method of claim 1 wherein the pH of the aqueous solution containing the germanium compound ranges from between about pH 4.5 to about pH 6.
- 6. The method of claim 1 wherein the pH of the aqueous solution containing the germanium compound ranges from between about pH 5 to about pH 6.
- 7. The method of claim 1 wherein the concentration of the germanium compound ranges from between about 100 ppm to about 30,000 ppm.
- 8. The method of claim 1 wherein the concentration of the germanium compound ranges from between about 500 ppm to about 25,000 ppm.
- 9. The method of claim 1 wherein the concentration of the germanium compound ranges from between about 1,000 ppm to about 20,000 ppm.
- 10. The method of claim 1 wherein the germanium compound contained in said aqueous solution is substantially pure.
- 11. The method of claim 1 wherein said yeast cells are edible.
- 12. The method of claim 11 wherein said yeast is selected from the group consisting of Saccharomyces cerevisae, Torula Utilis, and Saccharomyces uvarum.
- 13. The method of claim 1 wherein said growth medium includes nutrients selected from the group consisting of molasses, malt extract broth, potato dextrose broth, potassium chloride, magnesium sulfate, nitrogen, ammonium dihydrogen phosphate, ammonia, and phosphoric acid.
- 14. The method of claim 1 wherein said incubating step lasts from about 4 to about 50 hours.
- 15. The method of claim 1 wherein said incubating step is conducted at a temperature from between about 20.degree. C. and about 40.degree. C.
- 16. The method of claim 1 wherein said incubating step is conducted at a temperature from between about 25.degree. C. to about 35.degree. C.
- 17. The method of claim 1 wherein said incubating step is conducted at a temperature from between about 28.degree. C. to about 32.degree. C.
- 18. A dried yeast product containing germanium produced by the method of claim 1.
- 19. The dried yeast product of claim 18 having a concentration of intracellular germanium from about 500 .mu.g to about 3,000 .mu.g germanium per gram of dried yeast solids.
- 20. A dietary supplement comprising the dried yeast product of claim 18 in a suitable carrier.
- 21. A method of supplementing the human diet comprising administering an effective amount of the dried yeast product of claim 18 in a suitable carrier.
- 22. The method of claim 21 wherein the effective amount of the dried yeast product is between about 50 .mu.g/dose to about 200 .mu.g/dose.
CROSS-REFERENCE TO RELATED APPLICATION
The is a continuation-in-part of co-pending provisional application Ser. No. 60/006,339 filed Nov. 8, 1995.
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Non-Patent Literature Citations (1)
Entry |
Barnett J.A. et al. Yeasts Characteristics and Identification, Cambridge University Press. 2d Ed. 1990, pp. 595-597. |