Claims
- 1. An isolated polypeptide having glucose oxidase activity which is obtained from a strain of Cladosporium which has more than about 75% of maximum activity between about pH 5-8, determined at about 30.degree. C. with D-glucose as substrate, has an amino acid sequence which has at least about 80% identity with the amino acid sequence set forth in SEQ ID NO:1; and is encoded by a nucleic acid sequence which hybridizes with the nucleic acid sequence set forth in SEQ ID NOS:2, or 4 when prehybridized and subsequently hybridized at 42.degree. C. in 5.times.SSPE, 0.3% SDS, 200 ug/ml sheared and denatured salmon sperm DNA and 35% formamide.
- 2. The polypeptide of claim 1 in which said polypeptide is obtained from a strain of Cladosporium oxysporum.
- 3. The polypeptide of claim 1 in which said polypeptide is obtained from a strain of Cladosporium oxysporum, designated as CBS 163.94.
- 4. The polypeptide of claim 1 in which said polypeptide has an amino acid sequence set forth in SEQ ID NO:1.
- 5. The polypeptide of claim 1 in which said polypeptide has a pH optimum of about 6-7.
- 6. A method for producing the glucose oxidase of claim 1 comprising
- (a) fermenting a Cladosporium strain to produce a supernatant comprising the glucose oxidase; and
- (b) recovering the glucose oxidase.
- 7. A glucose oxidase obtained according to the method of claim 6.
- 8. A laundry detergent composition comprising surfactant, the glucose oxidase of claim 1, and a substrate for the glucose oxidase.
- 9. The laundry detergent composition according to claim 8, which additionally comprises a peroxidase.
- 10. The laundry detergent composition according to claim 9, which additionally comprises an oxidizable substrate selected from the group consisting of an organic compound, a phenolic compound, and p-hydroxybenzenesulfonic acid.
- 11. A dishwasing detergent composition comprising said glucose oxidase of claim 1 and a bleach precursor or peroxy acid, and substrate for glucose oxidase.
- 12. A method for strengthening gluten quality in dough comprising adding to the dough the glucose oxidase of claim 1 in an amount effective to strengthen gluten quality in the dough.
- 13. A method for removing bacteria from toothpaste, mouthwash, denture cleaner, liquid soap, skin care creams and lotions, hair care and body care formulations and solutions for cleaning contact lenses comprising adding the glucose oxidase of claim 1 to the toothpaste, mouthwash, denture cleaner, liquid soap, skin care creams and lotions, hair care and body care formulations.
Priority Claims (1)
Number |
Date |
Country |
Kind |
504/94 |
May 1994 |
DKX |
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Parent Case Info
This application is a continuation-in-part of application Ser. No. 08/446,645, filed May 25, 1995, which is a continuation-in-part of PCT/DK95/00178 May 3, 1995, the contents of which are incorporated herein by reference.
US Referenced Citations (4)
Foreign Referenced Citations (2)
Number |
Date |
Country |
0 338 452 |
Oct 1989 |
EPX |
8912675 |
Dec 1989 |
WOX |
Non-Patent Literature Citations (3)
Entry |
Oji Paper Co., JP 6141854, Dialog Abs. No. 009937307 (1994). |
Arica et al., J.Chem. Technol. Biotechnol., vol. 58(3) pp. 287-292, 1993. |
MPSRCH SEQ Searches, Headings: US-08-746-283-1.rag, pp. 1-2; US-08-746-283-2.rge, pp. 1-2. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
446645 |
May 1995 |
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