GLUTAMATE AGGRECANASE INHIBITORS

Information

  • Patent Application
  • 20100010012
  • Publication Number
    20100010012
  • Date Filed
    May 14, 2009
    15 years ago
  • Date Published
    January 14, 2010
    14 years ago
Abstract
The present invention relates to modulators of metalloproteinase activity.
Description

Throughout this application, various publications are referenced. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art as known to those skilled therein as of the date of the invention described and claimed herein.


This patent disclosure contains material that is subject to copyright protection. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure, as it appears in the U.S. Patent and Trademark Office patent file or records, but otherwise reserves any and all copyright rights whatsoever.


FIELD OF THE INVENTION

The present invention relates to compounds and their use as, for example, metalloproteinase inhibitors.


BACKGROUND OF THE INVENTION

Metalloproteinases, including matrix metalloproteinases and aggrecanases, are known to have a role in the breakdown of connective tissue. Matrix metalloproteinases (“MMPs”) constitute a superfamily of proteolytic enzymes that are genetically related and capable of degrading almost all the constituents of extracellular matrix and basement membrane that restrict cell movement. Aggrecanases are members of the ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs) family of proteins. Aggrecanase-1 and aggrecanase-2 have been designated ADAMTS-4 and ADAMTS-5, respectively (Tang B L, Int J Biochem Cell Biol 2001, 33, 33-44).


The ADAMTS family is involved in cleaving aggrecan, a cartilage component also known as the large aggregating chondroitin sulphate proteoglycan (Abbaszade I et al., J Biol Chem 1999, 274, 23443-23450), procollagen processing (Colige A et al., Proc Natl Acad Sci USA 1997, 94, 2374-2379), angiogenesis (Vazquez F et al., J Biol Chem 1999, 274, 23349-23357), inflammation (Kuno K et al., J Biol Chem 1997, 272, 556-562) and tumor invasion (Masui T. et al., J Biol Chem 1997, 272, 556-562). MMPs have been shown to cleave aggrecan as well.


The loss of aggrecan has been implicated in the degradation of articular cartilage in arthritic diseases, for example osteoarthritis is a debilitating disease which affects at least 30 million Americans. Degradation of articular cartilage and the resulting chronic pain can severely reduce quality of life. An early and important characteristic of the osteoarthritic process is loss of aggrecan from the extracellular matrix, resulting in deficiencies in the biomechanical characteristics of the cartilage. Likewise, MMPs and aggrecanases are known to play a role in many disorders in which extracellular protein degradation or destruction occurs, such as cancer, asthma, chronic obstructive pulmonary disease (“COPD”), atherosclerosis, age-related macular degeneration, myocardial infarction, corneal ulceration and other ocular surface diseases, hepatitis, aortic aneurysms, tendonitis, central nervous system diseases, abnormal wound healing, angiogenesis, restenosis, cirrhosis, multiple sclerosis, glomerulonephritis, graft versus host disease, diabetes, inflammatory bowel disease, shock, invertebral disc degeneration, stroke, osteopenia, and periodontal diseases.


Therefore, metalloproteinase inhibitors, including inhibitors of MMPs and aggrecanases, are needed.


The present invention is directed to these and other important ends.


SUMMARY OF THE INVENTION

In one embodiment, the invention provides compounds of the Formula (I):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • W is —C(O)—, —OC(O)—, —NHC(O)—, —C(O)O—, or —C(O)NH—;

    • R1 is phenyl, heteroaryl, biphenyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each optionally substituted with one or more of R5 or R6, and when R1 is substituted with more than one of R5 or R6, the substituents can be identical or different;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R3 is —CO2H, —CONH2, —CONHOH, —CONHSO2R7, tetrazole, —SO2NHR7, —SO3H, —PO(OH)NH2, —PO(OH)OR7, —CONHR7, —COOR7, an acid mimetic group, or a 5 or 6-membered heterocycloalkyl or heteroaryl containing 1 to 4 heteroatoms selected from O, N, S;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl;

    • m is 0-4;

    • n is 0-4; and

    • p is 0-2.





In another embodiment, the invention provides compounds of the Formula (II):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)pR11, —OH, or —O—(C1-C6) alkyl;

    • R14 and R15 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, heterocycloalkyl —(CH2)n-aryl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, or heteroaryl optionally substituted with one or more R12; or R14 and R15 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • p is 0-2, and

    • R1, R11, R12, and n are as described above for compounds of the Formula (I).





In another embodiment, the compounds or pharmaceutically acceptable salts of the compounds of Formula (I) or Formula (II) are useful as pharmaceutical compositions comprising compounds or pharmaceutically acceptable salts of compounds of Formula (I) or Formula (II) and a pharmaceutically acceptable carrier.


In one embodiment, the compounds or pharmaceutically acceptable salts of the compounds of the Formula (I) or Formula (II) are useful as metalloproteinase modulators.


In one embodiment, the invention provides methods for treating a metalloproteinase-related disorder, comprising administering to an animal in need thereof the compounds or pharmaceutically acceptable salts of compounds of Formula (I) or Formula (II) in an amount effective to treat a metalloproteinase-related disorder.


In one embodiment, the invention provides methods of synthesizing the compounds or pharmaceutically acceptable salts of compounds of Formula (I) or Formula (II). In another embodiment, the invention provides compounds or pharmaceutically acceptable salts of compounds of Formula (I) or Formula (II) made by particular processes.





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 illustrates inhibition of aggecan degradation by test compound 8OO.



FIG. 2 illustrates the effect of test compound 8OO on aggreecan metabolism.





DESCRIPTION OF THE INVENTION

All recitations of a group, such as alkyl, are understood for the purposes of this specification to encompass both substituted and unsubstituted forms.


The term “alkyl”, as used herein, whether used alone or as part of another group, refers to a substituted or unsubstituted aliphatic hydrocarbon chain and includes, but is not limited to, straight and branched chains containing from 1 to 12 carbon atoms, or in some instances, from 1 to 6 carbon atoms, unless explicitly specified otherwise. For example, methyl, ethyl, propyl, isopropyl, butyl, i-butyl and t-butyl are encompassed by the term “alkyl.” (C1-C6)-alkyl includes straight and branched chain aliphatic groups having from 1 to 6 carbons. Specifically included within the definition of “alkyl” are those aliphatic hydrocarbon chains that are optionally substituted. The alkyl may suitably be a (C1-C3)-alkyl. In one embodiment, an alkyl is optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The carbon number as used in the definitions herein refers to carbon backbone and carbon branching, but does not include carbon atoms of the substituents, such as alkoxy substitutions and the like.


The term “alkenyl”, as used herein, whether used alone or as part of another group, refers to a substituted or unsubstituted hydrocarbon chain and includes, but is not limited to, straight and branched chains having 2 to 8 carbon atoms e.g. 2 to 6 carbon atoms and containing at least one double bond. In one embodiment, the alkenyl moiety has 1 or 2 double bonds. Such alkenyl moieties may exist in the E or Z conformations and the compounds of this invention include both conformations. (C2-C6) alkenyl includes a 2 to 6 carbon straight or branched chain having at least one carbon-carbon double bond. Specifically included within the definition of “alkenyl” are those aliphatic hydrocarbon chains that are optionally substituted. In one embodiment, a heteroatom, such as O, S or N, attached to an alkenyl is not attached to a carbon atom that is bonded to a double bond. In one embodiment, an alkenyl is optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “alkynyl”, as used herein, whether used alone or as part of another group, refers to a hydrocarbon moiety containing at least one carbon-carbon triple bond. (C2-C6) alkynyl includes a 2 to 6 carbon straight or branched chain having at least one carbon-carbon triple bond. In one embodiment, an alkynyl is optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “cycloalkyl” refers to a monocyclic, bicyclic, tricyclic, fused, bridged, or spiro monovalent saturated or unsaturated non-aromatichydrocarbon ring system of 3-15 carbon atoms e.g. 3 to 6 carbon atoms. Any suitable ring position of the cycloalkyl moiety may be covalently linked to the defined chemical structure. Examples of cycloalkyl moieties include, but are not limited to, cyclopropyl, cyclopropylmethyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, norbornyl, adamantyl, spiro[4.5]decanyl, and homologs, isomers, and the like. C3-C6 cycloalkyl includes monocyclic, saturated rings of 3 to 6 carbons. In one embodiment, a cycloalkyl is optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl. Cycloalkylalkyl when used herein refers to a (C1-C6)-alkyl group as defined above substituted by a C3 to C15 cycloalkyl group as defined above e.g. cyclohexylmethyl and cyclohexylethyl.


“Heteroaryl” refers to a 5 to 6 membered aromatic heterocyclic ring which contains from 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur atoms in the ring and may be fused with a carbocyclic or heterocyclic ring at any possible position (e.g. fused to one or more carbocyclic or heterocyclic rings, each having 5-8 ring atoms, the fused heterocyclic ring containing from 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur atoms in the ring) e.g. it is suitably a bicyclic or tricyclic ring. Exemplary heteroaryl groups include, but are not limited to, furanyl, furazanyl, homopiperazinyl, imidazolinyl, isothiazolyl, isoxazolyl, oxadiazolyl, oxazolyl, pyrimidinyl, phenanthridinyl, pyranyl, pyrazinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridooxazolyl, pyridoimidazolyl, pyridothiazolyl, pyridinyl, pyrimidinyl, pyrrolinyl, thiadiazinyl, thiadiazolyl, thienyl, thienothiazolyl, thienooxazolyl, thienoimidazolyl, thiophenyl, triazinyl, and triazolyl. In one embodiment, a heteroaryl is optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


“Heterocycloalkyl” refers to a 5 to 7-membered saturated or unsaturated non aromatic ring containing carbon atoms and from 1 to 4 heteroatoms selected from N, O, and S which may suitably be monocyclic or may be fused to one or more further rings to provide a polycyclic moiety e.g. a bicyclic or tricyclic moiety. The further ring(s) may be carbocyclic rings(s) or they may contain from 1 to 4 further heteroatoms selected from N, O and S, preferably they are carbocyclic rings. The further ring(s) may be saturated, unsaturated or aromatic rings(s), preferably they are non-aromatic rings) Exemplary heterocycloalkyl groups include, but are not limited to, azepanyl, azetidinyl, aziridinyl, imidazolidinyl, morpholinyl, oxazolidinyl, oxazolidinyl, piperazinyl, piperidinyl, pyrazolidinyl, pyrrolidinyl, quinuclidinyl, tetrahydrofuranyl, and thiomorpholinyl. In one embodiment, a heterocycloalkyl is optionally substituted with one or more of the following: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “aryl” as used herein as a group or part of a group refers to an aromatic carbocyclic ring system, e.g., of from 6 to 14 carbon atoms such as phenyl, which may be optionally substituted. An aryl group may be fused with a carbocyclic or heterocyclic ring at any possible position (e.g. fused to one or more carbocyclic or heterocyclic rings, each having 5-8 ring atoms, the fused heterocyclic ring containing from 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur atoms in the ring). “Phenyl”, as used herein, whether used alone or as part of another group, refers to a substituted or unsubstituted phenyl group. The aryl fused with a carbocyclic ring may suitably be a bicyclic aryl or a tricyclic aryl. In one embodiment, an aryl group such as phenyl is optionally substituted with one or more of the following: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “biphenyl” as used herein refers to two phenyl groups connected at one carbon site on each ring. In one embodiment, one or both phenyl groups is independently optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “biaryl” as used herein refers to two aryl groups connected at one carbon site on each ring. In one embodiment, one or both aryl groups is independently optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “bicyclic aryl” as used herein refers to two fused carbocyclic groups, wherein one or both of the carbocyclic groups is aromatic. For example, a bicyclic aryl can contain from 8 to 12 ring atoms. In one embodiment, one or both carbocyclic groups of the bicyclic aryl is independently optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “tricyclic aryl” as used herein refers to three fused carbocyclic groups, wherein two or three of the carbocyclic groups is aromatic. For example, a tricyclic aryl can contain from 13 to 18 ring atoms. In one embodiment, one or more of the carbocyclic groups of the tricyclic aryl are independently optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “bicyclic heteroaryl” as used herein refers to two fused cyclic groups, wherein one or both of the cyclic groups is aromatic and contains one to four heteroatoms selected from O, S, and N. For example, a bicyclic heteroaryl can contain from 8 to 12 ring atoms, and from 1 to 3 heteroatoms selected from O, N, and S in each ring. In one embodiment, one or both cyclic groups is independently optionally substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


The term “tricyclic heteroaryl” as used herein refers to three fused cyclic groups, wherein two or three of the cyclic groups is aromatic and at least one aromatic group contains 1 to 4 heteroatoms selected from O, S, and N. For example, a tricyclic aryl can contain from 13 to 18 ring atoms, and from 1 to 3 heteroatoms selected from O, N, and S in each ring. In one embodiment, the cyclic groups are independently substituted with one or more of the following groups: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


An optionally substituted moiety may be substituted with one or more substituents, examples of which are as illustrated herein. In one embodiment, an “optionally substituted” moiety is substituted with one or more of the following: —V-halogen, —V—(C1-C6)-alkyl, —V—(C2-C6)-alkenyl, —V—(C2-C6)-alkynyl, —V—N(R′)2, methylenedioxo, ethylenedioxo, —V—NHSO2R′, —V—NR′COR′, —V—NHCO2R′, —V—NO2, —V—SO2N(R′)2, —V—SO2R′, —V—OR′, —V—COR′, —V—CO2R′, —V—CON(R′)2, or —V—CN, wherein each R′ is independently hydrogen, unsubstituted (C1-C6)-alkyl, or unsubstituted aryl; and wherein each V is independently a bond or (C1-C6)-alkyl.


When such moieties are substituted, for example, they may typically be mono-, di-, tri- or persubstituted. Examples for a halogen substituent include 1-bromo vinyl, 1-fluoro vinyl, 1,2-difluoro vinyl, 2,2-difluorovinyl, 1,2,2-trifluorovinyl, 1,2-dibromo ethane, 1,2 difluoro ethane, 1-fluoro-2-bromo ethane, CF2F3, CF2CF2CF3, and the like.


The term halogen includes bromine, chlorine, fluorine, and iodine.


For the sake of simplicity, connection points (“−”) are not depicted. When an atom or compound is described to define a variable, it is understood that it is intended to replace the variable in a manner to satisfy the valency of the atom or compound. For example, if “X*” was C(R*)═C(R*), both carbon atoms form a part of the ring in order to satisfy their respective valences. Likewise, when divalent substituents are presented, it is understood that they are not limited to the order listed, for example, as used in this specification “OCH2” encompasses CH2O and OCH2.


The term “administer”, “administering”, or “administration”, as used herein refers to either directly administering a compound or pharmaceutically acceptable salt of the compound or a composition to a animal, or administering a prodrug derivative or analog of the compound or pharmaceutically acceptable salt of the compound or composition to the animal, which can form an equivalent amount of active compound within the animal's body.


The term “animal” as used herein includes, without limitation, a human, mouse, rat, guinea pig, dog, cat, horse, cow, pig, monkey, chimpanzee, baboon, or rhesus. In one embodiment, the animal is a mammal. In another embodiment, the animal is a human.


The term “amine protecting group” as used herein refers to a moiety that temporarily blocks an amine reactive site in a compound. Generally, this is done so that a chemical reaction can be carried out at another reactive site in a multifunctional compound or to otherwise stabilize the amine. In one embodiment, an amine protecting group is selectively removable by a chemical reaction. An exemplary amine protecting group is a 9-fluorenylmethoxycarbonyl protecting group. Another exemplary amine protecting group is a carbamate protecting group. Carbamate protecting groups include, without limitation, t-butyl carbamate, methyl carbamate, ethyl carbamate, 2,2,2-trichloroethyl carbamate, 2-(trimethylsilyl)ethyl carbamate, 1,1-dimethyl-2,2,2-trichloroethyl carbamate, benzyl carbamate, p-methoxybenzyl carbamate, p-nitrobenzylcarbamate, p-bromobenzyl carbamate, p-chlorobenzyl carbamate, and 2,4-dichlorobenzyl carbamate. See, Greene and Wuts, Protecting Groups in Organic Synthesis, Second Edition, John Wiley & Sons (1991).


The term “carboxylic acid protecting group” as used herein refers to a moiety that temporarily blocks a carboxylic acid reactive site in a compound. Generally, this is done so that a chemical reaction can be carried out at another reactive site in a multifunctional compound or to otherwise stabilize the carboxylic acid. In one embodiment, a carboxylic acid protecting group is selectively removable by a chemical reaction. An exemplary carboxylic acid protecting group is an alkyl ester protecting group, such as a tert-butyl ester.


The term “acid mimetic group” as used herein refers to a moiety that has chemical and physical similarities producing broadly similar biological properties to an acid. In one embodiment, an acid mimetic group is a 5 or 6-membered heterocycle containing 1 to 4 heteroatoms selected from O, N, S. Exemplary acid mimetic groups include:







The term “conditions effective to” as used herein refers to synthetic reaction conditions which will be apparent to those skilled in the art of synthetic organic chemistry.


The term “effective amount” as used herein refers to an amount of a compound or pharmaceutically acceptable salt of a compound that, when administered to an animal, is effective to prevent, to at least partially ameliorate, or to cure, a condition from which the animal suffers or is suspected to suffer.


The term “metalloproteinase-related disorder” used herein refers to a condition for which it would be beneficial to modulate activity of the metalloproteinase. Exemplary metalloproteinase-related disorders include, without limitation, arthritic disorders, osteoarthritis, cancer, rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, atherosclerosis, age-related macular degeneration, myocardial infarction, corneal ulceration and other ocular surface diseases, hepatitis, aortic aneurysms, tendonitis, central nervous system diseases, abnormal wound healing, angiogenesis, restenosis, cirrhosis, multiple sclerosis, glomerulonephritis, graft versus host disease, diabetes, inflammatory bowel disease, shock, invertebral disc degeneration, stroke, osteopenia, and periodontal diseases.


The term “metalloproteinase modulator” refers to a compound that is capable of modulating the expression of a metalloproteinase. For example, a metalloproteinase modulator may enhance metalloproteinase expression. A metalloproteinase modulator may also be an inhibitor of a metalloproteinase.


The term “isolated and purified” as used herein refers to an isolate that is separate from other components of a reaction mixture or a natural source. In certain embodiments, the isolate contains at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or at least about 98% of the compound or pharmaceutically acceptable salt of the compound by weight of the isolate.


As used herein, a compound of the invention includes a pharmaceutically acceptable salt thereof. The term “pharmaceutically acceptable salt” as used herein refers to a salt of an acid and a basic nitrogen atom of a compound of the present invention. Exemplary salts include, but are not limited to, sulfate, citrate, acetate, oxalate, chloride, hydrochloride, bromide, hydrobromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, camphorsulfonate, napthalenesulfonate, propionate, succinate, fumarate, maleate, malonate, mandelate, malate, phthalate, and pamoate. The term “pharmaceutically acceptable salt” as used herein also refers to a salt of a compound of the present invention having an acidic functional group, such as a carboxylic acid functional group, and a base. Exemplary bases include, but are not limited to, hydroxide of alkali metals including sodium, potassium, and lithium; hydroxides of alkaline earth metals such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, organic amines such as unsubstituted or hydroxyl-substituted mono-, di-, or tri-alkylamines, dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-OH—(C1-C6)-alkylamine), such as N,N-dimethyl-N-(2-hydroxyethyl)amine or tri-(2-hydroxyethyl)amine; N-methyl-D-glucamine; morpholine; thiomorpholine; piperidine; pyrrolidine; and amino acids such as arginine, lysine, and the like. The term “pharmaceutically acceptable salt” also includes a hydrate of a compound of the present invention.


The term “substantially free of its corresponding opposite enantiomer” as used herein means that the compound contains no more than about 10% by weight of its corresponding opposite enantiomer. In other embodiments, the compound that is substantially free of its corresponding opposite enantiomer contains no more than about 5%, no more than about 1%, no more than about 0.5%, or no more than about 0.1% by weight of its corresponding opposite enantiomer. An enantiomer that is substantially free of its corresponding opposite enantiomer includes a compound that has been isolated and purified or has been prepared substantially free of its corresponding opposite enantiomer.


The term “tautomer” as used herein refers to compounds produced by the phenomenon wherein a proton of one atom of a molecule shifts to another atom. See, Jerry March, Advanced Organic Chemistry: Reactions, Mechanisms and Structures, Fourth Edition, John Wiley & Sons, pages 69-74 (1992).


The following abbreviations as used herein mean: Ac is acetate; boc is t-butyl carbamate; Bu is butyl; DIEA is diisopropylethylamine; DMF is dimethylformamide; DMSO is dimethylsulfoxide; ESI is electrospray ionization; Et is ethyl; HPLC is high pressure liquid chromatography; HRMS is high resolution mass spectrometry; Me is methyl; MS is mass spectrometry; m/z is mass-to-charge ratio; r.t. is retention time; TFA is trifluoroacetic acid; THF is tetrahydrofuran; FMOC is 9-fluorenylmethoxycarbonyl; DEA is diethylamine; DME is dimethoxyethane; Bop is benzotriazole-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate; PyBop is benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate; HBTU is O-benzotriazole-N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate; and EDC is 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride.


Compounds and Pharmaceutically Acceptable Salts of Compounds of the Invention

The compounds or pharmaceutically acceptable salts of compounds of the present invention can contain an asymmetric carbon atom and some of the compounds or pharmaceutically acceptable salts of compounds of the invention can contain one or more asymmetric centers, and can thus give rise to optical isomers and diastereomers. While depicted without respect to stereochemistry in the compounds or pharmaceutically acceptable salts of compounds of the present invention, the present invention includes such optical isomers and diastereomers, as well as racemic and resolved, enantiomerically pure R and S stereoisomers, and also other mixtures of the R and S stereoisomers and pharmaceutically acceptable salts thereof. Where a stereoisomer is provided, it can in some embodiments be provided substantially free of its corresponding opposite enantiomer.


In addition, the compounds and pharmaceutically acceptable salts of compounds of the present invention can exist as tautomers. Such tautomers can be transient or isolatable as a stable product. These tautomers are within the scope of the present invention.


Prodrugs of the compounds or pharmaceutically acceptable salts of compounds are also within the scope of the present invention.


Compounds of the Formula (I)

In one embodiment, the present invention is directed to compounds of the Formula (I):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • W is —C(O)—, —OC(O)—, —NHC(O)—, —C(O)O—, or —C(O)NH—;

    • R1 is phenyl, heteroaryl, biphenyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each optionally substituted with one or more of R5 or R6, and when R1 is substituted with more than one of R5 or R6, the substituents can be identical or different;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R3 is —CO2H, —CONH2, —CONHOH, —CONHSO2R7, tetrazole, —SO2NHR7, —SO3H, —PO(OH)NH2, —PO(OH)OR7, —CONHR7, —COOR7, an acid mimetic group, or a 5 or 6-membered heterocycloalkyl or heteroaryl containing 1 to 4 heteroatoms selected from O, N, S;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl;

    • m is 0-4;

    • n is 0-4; and

    • p is 0-2.





In one embodiment, W is —CO.


In another embodiment, R3 is —CO2H.


In one embodiment, R1 is bicyclic aryl optionally substituted with one or more of R5 and R6.


In another embodiment, R1 is tricyclic aryl optionally substituted with one or more of R5 and R6.


In one embodiment, R1 is biphenyl optionally substituted with one or more of R5 and R6.


In one embodiment, m is 0-2. In another embodiment, m is 0.


In one embodiment, p is 0-2. In another embodiment, p is 2.


In another embodiment, m is 0 and p is 2.


In another embodiment, R3 is







In another embodiment, R5 is







wherein X′ is O, S, or NH.


In one embodiment, R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10.


In one embodiment, W is —CO, R2 is hydrogen, R3 is —CO2H, R1 is biphenyl optionally substituted with one or more of R5 and R6, R4 is —CONR9R10, m is 0, and p is 2.


In another embodiment, when R4 is other than hydrogen, the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.


In one embodiment, R2 is hydrogen, R4 is







and R12 is —F.

In one embodiment, R2 is hydrogen, R4 is







and each R12 is —OCH3.


In another embodiment, R2 is hydrogen; and R4 is







Compounds of the Formula (Ia)

In one embodiment, the invention is directed to compounds of the Formula (Ia):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • R1 is phenyl, heteroaryl, biphenyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each optionally substituted with one or more of R5 or R6, and when R1 is substituted with more than one of R5 or R6, the substituents can be identical or different;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; and

    • n is 0-4.





In one embodiment, R1 is bicyclic aryl optionally substituted with one or more of R5 and R6.


In another embodiment, R1 is tricyclic aryl optionally substituted with one or more of R5 and R6.


In one embodiment, R1 is biphenyl optionally substituted with one or more of R5 and R6.


In one embodiment, R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10.


In one embodiment, R1 is biphenyl optionally substituted with one or more of R5 and R6, and R4 is —CONR9R10.


In another embodiment, R5 is







wherein X′ is O, S, or NH.


In another embodiment, when R4 is other than hydrogen, the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.


In one embodiment, R2 is hydrogen, R4 is







and R12 is —F.

In one embodiment, R2 is hydrogen, R4 is







and each R12 is —OCH3.


In another embodiment, R2 is hydrogen; and R4 is







Compounds of the Formula (Ib)

In one embodiment, the invention is directed to compounds of the Formula (Ib):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • X, Y, and Z are each independently (CH2)n, O, S, NR7, CO, or SO2; or X, Y, and Z form a benzene in ring B; ring A and ring B are each independently optionally substituted with one or more of R5 and R6;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; and

    • n is 0-4.





In one embodiment, R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10.


In another embodiment, when R4 is other than hydrogen, the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.


In one embodiment, R2 is hydrogen, R4 is







and R12 is —F.

In one embodiment, R2 is hydrogen; R4 is







And each R12 is —OCH3.


In another embodiment, R2 is hydrogen; and R4 is







Compounds of the Formula (Ic)

In one embodiment, the invention is directed to compounds of the Formula (Ic):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • X, Y, and Z are each independently (CH2)n, O, S, NR7, CO, or SO2; or X, Y, and Z form a benzene in ring B; ring A, ring B, and ring C are each independently optionally substituted with one or more of R5 and R6;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; and

    • n is 0-4.





In one embodiment, R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10.


In another embodiment, when R4 is other than hydrogen, the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.


In one embodiment, R2 is hydrogen, R4 is







and R12 is —F.

In one embodiment, R2 is hydrogen; R4 is







and each R12 is —OCH3.


In another embodiment, R2 is hydrogen; and R4 is







Compounds of the Formula (Id)

In one embodiment, the invention is directed to compounds of the Formula (Id):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • ring A and ring B are each independently optionally substituted with one or more of R5 and R6;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)nR11, —OH, or —O—(C1-C6) alkyl;

    • R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O—(C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O—(C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R9 and R10 are each independently hydrogen, (C1-C6) alkyl, (C1-C6) alkyl-OH, (C1-C6) alkyl-O—(C1-C6) alkyl, aryl, cycloalkyl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more R12; or R9 and R10 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; and

    • n is 0-4.





In one embodiment, R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10.


In another embodiment, when R4 is other than hydrogen, the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.


In one embodiment, R2 is hydrogen, R4 is







and R12 is —F.

In one embodiment, R2 is hydrogen; R4 is







and each R12 is —OCH3.


In another embodiment, R2 is hydrogen; and R4 is







Compounds of the Formula (II)

In another embodiment, the invention provides compounds of the Formula (II):









    • and pharmaceutically acceptable salts thereof,

    • wherein

    • R1 is phenyl, (C1-C6) alkyl substituted with phenyl, heteroaryl, biphenyl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl, each optionally substituted with one or more of R5 or R6, and when R1 is substituted with more than one of R5 or R6, the substituents can be identical or different;

    • R2 is hydrogen, (C1-C6) alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, —(CH2)pR11, —OH, or —O—(C1-C6) alkyl;

    • R5 is aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, —S-heteroaryl, —NH-aryl, —NH-heteroaryl, —CO—(C1-C6) alkyl, —CO-aryl, —CO-heteroaryl, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2NH-aryl, —SO2NH-heteroaryl, —NHSO2—(C1-C6) alkyl, —NHSO2-aryl, —NHSO2-heteroaryl, —NHCO-aryl, —NHCO-heteroaryl, —CONH-aryl, —CONH-heteroaryl, (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, or —CONH—(C3-C6) cycloalkyl; each alkyl, aryl, cycloalkyl, or heteroaryl optionally substituted with one or more of R6, and when R5 is substituted with more than one R6, the substituents can be identical or different;

    • R6 and R12 are each independently hydrogen, halogen, —CN, —OCF3, —CF3, —NO2, —OH, —SH, —NR7R8, —CONR7R8, —NR8COR7, —NR8CO2R7, —CO2R7, —COR7, —SO2—(C1-C6) alkyl, —SO2-aryl, —SO2-heteroaryl, —SO2R7, —NR7SO2R8, —SO2NR7R8; (C1-C6) alkyl, —O—(C1-C6) alkyl, —S—(C1-C6) alkyl, —NH—(C1-C6) alkyl, —NHCO—(C1-C6) alkyl, —CONH—(C1-C6) alkyl, —O—(C3-C6) cycloalkyl, —S—(C3-C6) cycloalkyl, —NH—(C3-C6) cycloalkyl, —NHCO—(C3-C6) cycloalkyl, —CONH—(C3-C6) cycloalkyl, heterocycloalkyl, —(C1-C6) alkyl-OR7, (C2-C6) alkynyl, (C2-C6) alkenyl, —O— (C1-C6) alkyl-(C3-C6) cycloalkyl, —O-alkenyl, —O— (C1-C6) alkyl substituted with aryl, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, —O-aryl, —O-heteroaryl, —S-aryl, or —S-heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, alkenyl, or alkynyl optionally substituted with one or more of R13;

    • R7 and R8 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl; or R7 and R8 together with the atom to which they are attached may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • R11 is aryl, heteroaryl, or cycloalkyl;

    • R13 is halogen, —O—(C1-C6) alkyl, —CO2H, —OH, —CF3, hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, cycloalkyl substituted with —OH, aryl substituted with —NH2, aryl substituted with —O—(C1-C6) alkyl, —(CH2)n-aryl, or —(CH2)n-heteroaryl;

    • R14 and R15 are each independently hydrogen, (C1-C6) alkyl, aryl, heteroaryl, (C2-C6) alkenyl, (C2-C6) alkynyl, cycloalkyl, heterocycloalkyl —(CH2)n-aryl, bicyclic aryl, tricyclic aryl, bicyclic heteroaryl, or tricyclic heteroaryl; each alkyl, aryl, cycloalkyl, heterocycloalkyl, or heteroaryl optionally substituted with one or more R12; or R14 and R15 together may form a five- to seven-membered cyclic group containing up to 3 heteroatoms selected from N, O, or S;

    • n is 0-4, and

    • p is 0-2.





In one embodiment, R1 is bicyclic aryl optionally substituted with one or more of R5 and R6.


In another embodiment, R1 is tricyclic aryl optionally substituted with one or more of R5 and R6.


In one embodiment, R1 is biphenyl optionally substituted with one or more of R5 and R6.


In another embodiment, R5 is







wherein X′ is O, S, or NH.


In another embodiment, R2 is hydrogen.


In one embodiment, the compound of Formula (I) is N2-(4-benzoylbenzoyl)-N1-benzyl-L-α-glutamine, N-(4-benzoylbenzoyl)-L-glutamic acid, N1-(1,3-benzodioxol-5-ylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-butyl-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopropyl-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclohexyl-L-α-glutamine, N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutamine, N1-benzyl-N2-[4-(2-furyl)benzoyl]-L-α-glutamine, N1-benzyl-N2-[(4′-ethynyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-fluorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclooctyl-L-α-glutamine, N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutamine, N1-benzyl-N2-[(2′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethoxybenzyl)-L-α-glutamine, N1-benzyl-N2-(4-pyridin-4-ylbenzoyl)-L-α-glutamine, N1-benzyl-N2-[(3′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(4′-ethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(3′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(2′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(2′,6′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopentyl-L-α-glutamine, N1-[2-(acetylamino)ethyl]-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N1-2-adamantyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N1-(2-adamantylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-(4-pyridin-2-ylbenzoyl)-L-α-glutamine, N1-benzyl-N2-(4-pyridin-3-ylbenzoyl)-L-α-glutamine, N1-benzyl-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3,4-dimethoxyphenyl)ethyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxyethyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(1-naphthylmethyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-furylmethyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dichlorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(trifluoromethoxy)benzyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(methylthio)benzyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dimethoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dichlorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-difluorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-[3-(trifluoromethoxy)benzyl]-L-glutamamide, N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-[2-(3-methoxyphenyl)ethyl]-L-glutamamide, N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-(3,4,5-trimethoxybenzyl)-L-glutamamide, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-dimethoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-dimethoxybenzyl)-N5-hydroxy-L-glutamamide, N1-benzyl-N2-[(4′-vinyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-L-α-glutamine, N1-benzyl-N2-[(4′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(4′-propoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(4′-butoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-{[4′-(cyclobutylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine, N1-benzyl-N2-{[4′-(cyclohexylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine, N2-{[4′-(allyloxy)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-N5-hydroxy-L-glutamamide, N2-(1,1′-biphenyl-4-ylacetyl)-N1-(3-methoxybenzyl)-L-α-glutamine, N2-[(3-fluorophenyl)acetyl]-N1-(3-methoxybenzyl)-L-α-glutamine, N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine, N1-benzyl-N2-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine, N1-benzyl-N2-({4′-[(4-fluorobenzoyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine, N2-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine, N1-benzyl-N2-(5-phenyl-2-furoyl)-L-α-glutamine, N1-benzyl-N2-[5-(3-ethoxyphenyl)-2-furoyl]-L-α-glutamine, N1-benzyl-N2-[5-(3,5-dimethylphenyl)-2-furoyl]-L-α-glutamine, N1-benzyl-N2-(2,2′-bifuran-5-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-(5-thien-2-yl-2-furoyl)-L-α-glutamine, N2-[(2,2′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-phenylethyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethyl)benzyl]-L-α-glutamine, N1-(4-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N1-(3-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(trifluoromethyl)benzyl]-L-α-glutamine, N1-benzyl-N2-[(2-fluoro-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-fluorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine, N1-benzyl-N2-(1,1′-biphenyl-3-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-(3-thien-2-ylbenzoyl)-L-α-glutamine, N1-benzyl-N2-[(3-chloro-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(3-fluoro-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-[(2,6-dimethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-chlorophenyl)ethyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-fluorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-difluorobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methylbenzyl)-L-α-glutamine, N1-benzyl-N2-({4′-[(3-methoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine, N1-benzyl-N2-({4′-[(3,5-dimethoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine, N1-benzyl-N2-{[4′-(2-naphthylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,3-dimethylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenylbutyl)-L-α-glutamine, N1-(2-benzylphenyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxy-1,1′-biphenyl-3-yl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-heptyl-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-vinylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-thien-2-ylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N1-benzyl-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine, N1-(3-methoxybenzyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-tert-butylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-iodobenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-vinylbenzyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(sec-butyl)-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(cyclopropylmethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[4-(dimethylamino)benzyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-bromophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(2-biphenyl-4-ylethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2′-ethoxybiphenyl-4-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-ethynylbiphenyl-4-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4-(2-thienyl)phenyl]ethyl}-L-α-glutamine, N2— (biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-2-ylphenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[3-(dimethylamino)benzyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-diphenylpropyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-dimethylbutyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-isopropyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(pyridin-4-ylmethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1,N1-diethyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethylpropyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-4-yl]ethyl}-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-3-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-3-yl]ethyl}-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-3-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-4-yl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-bromophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-pentyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-4-ylphenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(tert-butyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-{[4′-(trifluoromethyl)biphenyl-3-yl]methyl}-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(2,2-dimethylpropyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[(4′-ethynylbiphenyl-3-yl)methyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(3-pyridin-2-ylbenzyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-hexyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(1,3,3-tetramethylbutyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(2-morpholin-4-ylethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(2-hydroxy-1,1-dimethylethyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-fluorophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-thienyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-chlorophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-(5-hydroxypentyl)-L-α-glutamine, 3-[(biphenyl-4-ylmethyl)amino]-3-oxopropanoic acid, N2-(biphenyl-4-ylcarbonyl)-N1-propyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-pyridin-2-ylphenyl)ethyl]-L-α-glutamine, N2-2-naphthoyl-N1-(3-phenylpropyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(9H-fluoren-1-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine, N2-(9H-fluoren-1-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine, N2-(4-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-hexyl-L-α-glutamine, N2-(9H-fluoren-1-ylcarbonyl)-N1-hexyl-L-α-glutamine, N1-hexyl-N2-(4-phenoxybenzoyl)-L-α-glutamine, N1-hexyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3-phenylpropyl)-L-α-glutamine, N2-(4-phenoxybenzoyl)-N1-(3-phenylpropyl)-L-α-glutamine, N-[4-(pyrimidin-2-ylamino)benzoyl]-L-glutamic acid, N-(1,1′-biphenyl-4-ylcarbonyl)-D-glutamic acid, N-[4-(pyrimidin-2-ylamino)benzoyl]-L-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-D-α-glutamine, N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-hydroxybutyl)-L-α-glutamine, N1-benzyl-N2-(1,1-biphenyl-4-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-[4-(pyrimidin-2-ylamino)benzoyl]-L-α-glutamine, (4S)-4-[(1,1′-biphenyl-4-ylcarbonyl)amino]-5-hydroxypentanoic acid, N-({4′-[(phenylacetyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid, N-[(4′-{[(3-methyl-1-benzofuran-2-yl)carbonyl]amino}-1,1′-biphenyl-4-yl)carbonyl]-L-glutamic acid, N-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid, N-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid, N-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid, N-({4′-[(1-benzofuran-2-ylcarbonyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid, N1-(1,1-dimethyl-2-phenylethyl)-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine, N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine, N1-(6-hydroxyhexyl)-N2-(4-phenoxybenzoyl)-L-α-glutamine, N2-(9H-fluoren-9-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine, N2-(9H-fluoren-9-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(3-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(9H-fluoren-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N1-1-adamantyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N1-1-adamantyl-N2-(4-phenoxybenzoyl)-L-α-glutamine, N1-1-adamantyl-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine, N1-1-adamantyl-N2-(9H-fluoren-9-ylcarbonyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine, N1-(3-methylbenzyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N2-(9H-fluoren-9-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-propyl-L-α-glutamine, N1-benzyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N1-benzyl-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-(9H-fluoren-9-ylcarbonyl)-L-α-glutamine, N1-benzyl-N2-(3-phenoxybenzoyl)-L-α-glutamine, N1-(1-methyl-1-phenylethyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N2-(9H-fluoren-9-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutamine, N1-(1-methyl-1-phenylethyl)-N2-(3-phenoxybenzoyl)-L-α-glutamine, N2— (biphenyl-4-ylcarbonyl)-N1-[(1S)-1-phenylethyl]-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-[(1S)-1-phenylethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[(1S)-1-(4-fluorophenyl)ethyl]-L-α-glutamine, tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(1-phenylethyl)-L-α-glutaminate, N2-(biphenyl-4-ylcarbonyl)-N1-[(1R)-1-(4-fluorophenyl)ethyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[(1R)-1-phenylethyl]-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-[(1R)-1-phenylethyl]-L-α-glutamine, N2-(9H-fluoren-1-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine, N1-(6-hydroxyhexyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-9H-fluoren-9-yl-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(2′-ethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(4′-methoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(4-bromobenzoyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, 4′-{[((1S)-3-carboxy-1-{[(3,4,5-trimethoxybenzyl)amino]carbonyl}propyl)amino]carbonyl}biphenyl-3-carboxylic acid, N2-[(4′-ethylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-benzyl-N2-[4-(2-naphthyl)benzoyl]-L-α-glutamine, N2-[(3′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(2′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-(3,4,5-trimethoxybenzyl)-N2-[(3′,4′,5′-trimethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1-adamantylmethyl)-N2-(biphenyl-4-ylcarbonyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-[(3′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(2′-ethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(4-bromobenzoyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-[(1S)-1-benzyl-2-hydroxyethyl]-N2-(biphenyl-4-ylcarbonyl)-L-α-glutamine, N-[(1S)-1-benzyl-2-hydroxyethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-(4-bromobenzoyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-[(2′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N-methyl-N-(2-phenylethyl)-L-α-glutamine, N2-[(4′-sec-butylbiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-isopropylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-(1,1′:4′,1″-terphenyl-4-ylcarbonyl)-L-α-glutamine, N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-({3′-[(dimethylamino)carbonyl]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-[(3′,5′-dimethylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-N-(2-phenylethyl)-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-({4′-[(3,5-dimethylbenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(4′-{[3,5-bis(trifluoromethyl)benzyl]oxy}biphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(3′-isopropylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-[(4′-isopropylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-(1-adamantylmethyl)-N2-[4-(1-benzofuran-5-yl)benzoyl]-L-α-glutamine, N-(1-adamantylmethyl)-N2-[4-(1H-indol-5-yl)benzoyl]-L-α-glutamine, N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-isopropylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(4′-isobutylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(1,1:4′,1 terphenyl-4-ylcarbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-benzyl-N2-{[3′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-L-α-glutamine, N-benzyl-N2-({4′-[(3-fluorobenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-benzyl-N2-{[4′-(benzyloxy)biphenyl-4-yl]carbonyl}-L-α-glutamine, N-benzyl-N2-[4-(phenylethynyl)benzoyl]-L-α-glutamine, N-benzyl-N2-{4-[(9-hydroxy-9H-fluoren-9-yl)ethynyl]benzoyl}-L-α-glutamine, N-benzyl-N2-{4-[(1E)-3-oxo-3-phenylprop-1-en-1-yl]benzoyl}-L-α-glutamine, N-(3,4,5-trimethoxybenzyl)-N2-[(2′,4′,6′-trimethylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(6-hydroxyhexyl)-L-α-glutamine, N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-(4-bromobenzoyl)-N-(3-methylbenzyl)-L-α-glutamine, N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-(3-methylbenzyl)-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N-butyl-N-methyl-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N-butyl-N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N-[2-(3,4-dimethoxyphenyl)ethyl]-N-methyl-L-α-glutamine, N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(3,4-dimethoxyphenyl)ethyl]-N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-L-α-glutamine, N2-(9H-fluoren-2-ylcarbonyl)-N-[(2R)-2-phenylpropyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N-[(2R)-2-phenylpropyl]-L-α-glutamine, N-benzyl-N2-{4-[(4-hydroxycyclohexyl)ethynyl]benzoyl}-L-α-glutamine, N2-{4-[(3-aminophenyl)ethynyl]benzoyl}-N-benzyl-L-α-glutamine, N-benzyl-N2-[4-(3-hydroxy-3,3-diphenylprop-1-yn-1-yl)benzoyl]-L-α-glutamine, N-benzyl-N2-{4-[(3-methoxyphenyl)ethynyl]benzoyl}-L-α-glutamine, N2-[(3′-hydroxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-glutamamide, N2-({4′-[(3-tert-butylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-({4′-[(3,5-di-tert-butylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-ethylphenoxy)methyl]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-methoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(biphenyl-4ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,2-dimethylethyl]-N5-hydroxy-L-glutamanide, N2-[4-(5-bromo-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,5′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,6′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(3′,5′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,3′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-(4-pyrimidin-5-ylbenzoyl)-L-α-glutamine, N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-[(3′,5′-difluorobiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-(biphenyl-4-ylcarbonyl)-N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-L-α-glutamine, N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-L-α-glutamine, N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-{[4′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(2-furyl)benzoyl]-L-α-glutamine, N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine, N2-{[3′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine, N2-[4-(2,3-dihydro-1-benzofuran-5-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(3′-chloro-4′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-{[3′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(3-thienyl)benzoyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(5-methyl-2-thienyl)benzoyl]-L-α-glutamine, N2-[4-(5-chloro-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine, N2-(2,2′-bithien-5-ylcarbonyl)-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[6-(3-methoxyphenyl)pyridin-3-yl]carbonyl}-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine, N2-[(3-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-4-yl-2-thienyl)carbonyl]-L-α-glutamine, N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine, N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine, N2-(2,2′-bithien-5-ylcarbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-pyridin-4-yl-2-thienyl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(5-chloro-2-thienyl)benzoyl]-L-α-glutamine, N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(5-phenyl-2-furoyl)-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine, N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-(2,2′-bithien-5-ylcarbonyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(5-formyl-2-thienyl)benzoyl]-L-α-glutamine, N2-[4-(5-acetyl-2-thienyl)benzoyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-(5-phenyl-2-furoyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(5-formyl-2-thienyl)benzoyl]-L-α-glutamine, N2-[4-(5-acetyl-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[3-(5-chloro-2-thienyl)benzoyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[3-(2-thienyl)benzoyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine, N2-[4-(5-bromo-2-thienyl)benzoyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[4-(5-bromo-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine, N2-[3-(5-chloro-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[3-(2-thienyl)benzoyl]-L-α-glutamine, N2-[(3′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(4′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(4′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(3-phenyl-2-thienyl)carbonyl]-L-α-glutamine, N2-[(4′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(3′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine, N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(3-phenyl-2-thienyl)carbonyl]-L-α-glutamine, N2-(4-bromobenzoyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine, N2-[4-(5-chloro-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutamine, N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-{[5-(3,4-difluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-({4′-[(1,3-benzodioxol-5-yloxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine, N2-{[3′-(benzyloxy)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-({3′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-({3′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine, N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine, N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine, N-benzyl-N2-({4′-[(3′-methoxybiphenyl-3-yl)methoxy]biphenyl-4-yl}carbonyl)-L-α-glutamine, N2-({4′-[(3,5-dimethoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine, N2-({3′-[(3,5-dimethoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine, N2-[(3′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(3-methylbenzyl)-L-α-glutamine, N2-({3′-[(3-ethylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine, N-benzyl-N2-{[4′-(biphenyl-2-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine, or N-benzyl-N2-({4′-[(3′-methoxybiphenyl-2-yl)methoxy]biphenyl-4-yl}carbonyl)-L-α-glutamine.


Methods for Making the Compounds and Pharmaceutically Acceptable Salts of Compounds of the Invention

The compounds and pharmaceutically acceptable salts of compounds of the present invention can be prepared using a variety of methods starting from commercially available compounds, known compounds, or compounds prepared by known methods. General synthetic routes to many of the compounds of the invention are included in the following schemes. It is understood by those skilled in the art that protection and deprotection steps not shown in the Schemes may be required for these syntheses, and that the order of steps may be changed to accommodate functionality in the target molecule.


As used in the schemes, PG1 is an amine protecting group; PG2 is a carboxylic acid protecting group; and R1, R2, R6, R12, R13, R14, and R15 are defined as above.


General Synthetic Schemes for Preparation of Compounds






The amide formation between a carboxylic acid and an amine is carried out using a coupling reagent in a solvent such as DMF. Coupling reagents that may be used include benzotriazole-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate (Bop reagent), benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate (PyBop reagent), O-benzotriazole-N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDC), and other known or commercially available coupling reagents.


Cross coupling is generally referred to as Suzuki or Stille coupling reactions.


The amine protecting group is cleaved under mild conditions with an amine base to afford the free amine. A variety of amine bases may be used, including for example, diethylamine, piperidine, morpholine, dicyclohexylamine, p-dimethylaminopyridine, or diisopropylethylamine in a solvent, such as acetonitrile or DMF.


Hydrolysis of the carboxylic acid protecting group is carried out using TFA, NaOH, LiOH, potassium carbonate, or the like.


Scheme 1 demonstrates the preparation of compounds of the invention in two steps from N-(4-aminobenzoyl)-L-glutamic diethyl ester (1). Heating an aryl or heteroaryl chloride such as chloropyridine with N-(4-aminobenzoyl)-L-glutamic diethyl ester gave the diethyl ester (2), which was hydrolyzed with bases such as NaOH, LiOH, or the like to provide (3).







Scheme 2 provides the preparation of a compound of the invention in two steps from biphenylcarboxylic acid (4). Amide formation was carried out using a coupling reagent, such as EDC. Hydrolysis of the diethyl ester (5) under basic conditions gave the final product dicarboxylic acid (6).







Scheme 3 provides the preparation of compounds of the invention in two steps. Coupling of the amine and carboxylic acid (7) was carried out using a coupling reagent such as EDC. The tert-butyl protecting group on (8) was hydrolyzed in acidic conditions (TFA in dichloromethane) to provide the product (9).







Scheme 4 demonstrates the synthesis of a compound or pharmaceutically acceptable salt of a compound of the Formula (II). As shown in Scheme 4, a compound of the Formula (III) is treated with an amine under conditions effective to provide a compound of the Formula (IV). Hydrolysis of the alkyl ester provides a compound of the Formula (II).







Scheme 5 provides the synthesis of compounds of the Formula (II) following the general procedures set forth in Scheme 4. Coupling of commercially available amines, amines known in the literature, or other amines with biphenylcarboxylic acid (4, or fluorenecarboxylic acids or other carboxylic acids) can be carried out in EDC, PyBop/DIEA, or other coupling reagents as described above in the general scheme. Hydrolysis of the tert-butyl ester (10) was carried out using TFA. The second coupling reaction of the carboxylic acid (11) with a variety of amines was carried out using a similar procedure as employed in the first step. Finally, the methyl ester (12) was hydrolyzed with NaOH/MeOH in THF to provide (13).







Scheme 6 demonstrates the synthesis of a compound or pharmaceutically acceptable salt of a compound of the Formula (II). As shown in Scheme 6, a compound of the Formula (V) is treated with an amine under conditions effective to provide a compound of the Formula (VI). Hydrolysis of the amine protecting group provides a compound of the Formula (VII), which is coupled with a carboxylic acid under conditions effective to provide a compound of the Formula (VIII). Hydrolysis of the protected carboxylic acid provides a compound of the Formula (II).







Scheme 7 provides the synthesis of compounds of the Formula (II) following the general procedures set forth in Scheme 6. In scheme 7, Fmoc-glu(Obut)OH (14) was reacted with a known or commercially available primary or secondary amine. Hydrolysis of the Fmoc protecting group of (15) was carried out with diethylamine in acetonitrile. The amine (16) was then coupled with a carboxylic acid (e.g. 4-biphenylcarboxylic acid or fluorenecarboxylic acid) and the resulting tert-butyl ester (17) was hydrolyzed using TFA in dichloromethane to provide (18).







Scheme 8 demonstrates the preparation of compounds of the invention similar to the procedure described in scheme 7. Bromo or iodophenethylamine (or bromo or iodo benzylamine) was coupled with the Fmoc protected amino acid (14). Removal of the Fmoc protecting group of (19) was carried out in base and the resulting compound (20) was reacted with 4-biphenylcarboxylic acid (or fluorenecarboxylic acid or other carboxylic acid). Cross-coupling of the bromo or iodo intermediate (21) with a boronic ester or stannyl reagent (via Suzuki or Stille method) gave the coupling product t-butyl ester (22), which was then hydrolyzed to give the final carboxylic acid product (23).







Scheme 9 demonstrates the preparation of compounds of the invention in three steps from bromo compounds. Cross coupling of (25) with either a boronic ester or a stannyl compound using either Suzuki or Stille coupling gave the tert-butyl ester (26), which was then hydrolyzed to the carboxylic acid (27).







Scheme 10 provides compounds of the invention prepared in three or four steps from bromo compounds. Suzuki coupling of (25) with the 4-hydroxyphenylboronic acid gave the intermediate (28). Path A: Alkylation of the phenolic compound with an alkylhalide or benzyl halide provided (29), followed by acid hydrolysis to give the carboxylic acid (30). Path B: The brominated compound (31) was treated with an alcohol to give (32), followed by acid hydrolysis to give the carboxylic acid (33). Path C: The intermediate (28) was treated a bromobenzylbromide compound to provide (52), followed by treatment with phenylboronic acid to provide (53), which was then acid hydrolized to give (54).










Scheme 11 provides compounds of the invention prepared in a similar manner described in scheme 10, except 5-bromofuroic acid was used in the first coupling reaction step. The bromo intermediate (34) was then reacted with a boronic acid and hydrolysis of the resulting tert-butyl ester (35) in acid provided the carboxylic acid (36).







Scheme 12 provides compounds of the invention prepared according to the procedure described in scheme 11, except 6-bromonicotinic acid was used to couple with the amino intermediate (37) to afford (38).







Scheme 13 provides compounds of the invention prepared according to the procedure described in scheme 10, except 4-(4,4,5,5)-tetramethyl-1,3,2-dioxoborolan-2-yl)aniline was used in the Suzuki coupling reaction with (25). The amino intermediate (39) was then acylated with an acid chloride and the tert-butyl ester (40) was hydrolyzed in acidic conditions to give the desired carboxylic acid (41).







Scheme 14 demonstrates the preparation of compounds of the invention in four steps. The L-glutamic acid α-tert-butyl ester γ-ethyl ester was reacted with 4-biphenylcarboxylic acid (4, or other carboxylic acid) using EDC, PyBop, Bop, or other coupling agent. Hydrolysis of the alpha tert-butyl ester (42) in trifluoroacetic acid gave the alpha carboxylic acid intermediate (43) in good yield. Treatment of the acid with a base (e.g. triethylamine) and ethyl chloroformate followed by sodium borohydride gave the corresponding alcohol (44). The ethyl ester was then hydrolyzed in basic conditions to give the final product (45).







Scheme 15 provides compounds of the invention prepared in four steps from bromobenzoic acid. L-glutamic acid di-tert butyl ester was coupled with the bromobenzoic acid (46). Suzuki coupling of the bromo compound (47) with 4-(4,4,5,5)-tetramethyl-1,3,2-dioxoborolan-2-yl)aniline gave the amino intermediate (48) in good yield. Acylation of the amine with an acid chloride gave the acylated product (49), and hydrolysis of the tert-butyl ester in acidic conditions gave the final dicarboxylic acid (50).







Scheme 16 provides compounds of the invention prepared in a single step from a carboxylic acid. A carboxylic acid of the invention (17) is reacted with Bop, hydroxylamine hydrochloride, and DIEA to afford the hydroxamic acid (51).







One of skill in the art will recognize that Schemes 1-16 can be adapted to produce other compounds and pharmaceutically acceptable salts of compounds according to the present invention.


Therapeutic Administration

When administered to an animal, the compounds or pharmaceutically acceptable salts of the compounds of the invention can be administered neat or as a component of a composition that comprises a physiologically acceptable carrier or vehicle. A composition of the invention can be prepared using a method comprising admixing the compound or a pharmaceutically acceptable salt of the compound and a physiologically acceptable carrier, excipient, or diluent. Admixing can be accomplished using methods well known for admixing a compound or a pharmaceutically acceptable salt of the compound and a physiologically acceptable carrier, excipient, or diluent.


The present compositions, comprising compounds or pharmaceutically acceptable salts of the compounds of the invention can be administered orally. The compounds or pharmaceutically acceptable salts of compounds of the invention can also be administered by any other convenient route, for example, by infusion or bolus injection, by absorption through epithelial or mucocutaneous linings (e.g., oral, rectal, vaginal, and intestinal mucosa, etc.) and can be administered together with another therapeutic agent. Administration can be systemic or local. Various known delivery systems, including encapsulation in liposomes, microparticles, microcapsules, and capsules, can be used.


Methods of administration include, but are not limited to, intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, oral, sublingual, intracerebral, intravaginal, transdermal, rectal, by inhalation, or topical, particularly to the ears, nose, eyes, or skin. In some instances, administration will result of release of the compound or a pharmaceutically acceptable salt of the compound into the bloodstream. The mode of administration is left to the discretion of the practitioner.


In one embodiment, the compound or a pharmaceutically acceptable salt of the compound is administered orally.


In another embodiment, the compound or a pharmaceutically acceptable salt of the compound is administered intravenously.


In another embodiment, it may be desirable to administer the compound or a pharmaceutically acceptable salt of the compound locally. This can be achieved, for example, by local infusion during surgery, topical application, e.g., in conjunction with a wound dressing after surgery, by injection, by means of a catheter, by means of a suppository or edema, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as sialastic membranes, or fibers.


In certain embodiments, it can be desirable to introduce the compound or a pharmaceutically acceptable salt of the compound into the central nervous system, circulatory system or gastrointestinal tract by any suitable route, including intraventricular, intrathecal injection, paraspinal injection, epidural injection, enema, and by injection adjacent to the peripheral nerve. Intraventricular injection can be facilitated by an intraventricular catheter, for example, attached to a reservoir, such as an Ommaya reservoir.


Pulmonary administration can also be employed, e.g., by use of an inhaler or nebulizer, and formulation with an aerosolizing agent, or via perfusion in a fluorocarbon or synthetic pulmonary surfactant. In certain embodiments, the compound or a pharmaceutically acceptable salt of the compound can be formulated as a suppository, with traditional binders and excipients such as triglycerides.


In another embodiment, the compound or a pharmaceutically acceptable salt of the compound can be delivered in a vesicle, in particular a liposome (see Langer, Science 1990, 249, 1527-1533 and Treat et al., Liposomes in the Therapy of Infectious Disease and Cancer 1989, 317-327 and 353-365.


In yet another embodiment, the compound or a pharmaceutically acceptable salt of the compound can be delivered in a controlled-release system or sustained-release system (see, e.g., Goodson, in Medical Applications of Controlled Release 1984, vol. 2, 115-138). Other controlled or sustained-release systems discussed in the review by Langer, Science 1990, 249, 1527-1533 can be used. In one embodiment, a pump can be used (Langer, Science 1990, 249, 1527-1533; Sefton, CRC Crit. Ref. Biomed. Eng. 1987, 14, 201; Buchwald et al., Surgery 1980, 88, 507; and Saudek et al., N. Engl. J Med. 1989, 321, 574). In another embodiment, polymeric materials can be used (see Medical Applications of Controlled Release (Langer and Wise, eds., 1974); Controlled Drug Bioavailability, Drug Product Design and Performance (Smolen and Ball, eds., 1984); Ranger and Peppas, J. Macromol. Sci. Rev. Macromol. Chem. 1983, 2, 61; Levy et al., Science 1935, 228, 190; During et al., Ann. Neural. 1989, 25, 351; and Howard et al., J. Neurosurg. 1989, 71, 105).


In yet another embodiment, a controlled- or sustained-release system can be placed in proximity of a target of the compound or a pharmaceutically acceptable salt of the compound, e.g., the reproductive organs, thus requiring only a fraction of the systemic dose.


The present compositions can optionally comprise a suitable amount of a physiologically acceptable excipient.


Such physiologically acceptable excipients can be liquids, such as water and oils, including those of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. The physiologically acceptable excipients can be saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea and the like. In addition, auxiliary, stabilizing, thickening, lubricating, and coloring agents can be used. In one embodiment the physiologically acceptable excipients are sterile when administered to an animal. The physiologically acceptable excipient should be stable under the conditions of manufacture and storage and should be preserved against the contaminating action of microorganisms. Water is a particularly useful excipient when the compound or a pharmaceutically acceptable salt of the compound is administered intravenously. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid excipients, particularly for injectable solutions. Suitable physiologically acceptable excipients also include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like. The present compositions, if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents.


Liquid carriers may be used in preparing solutions, suspensions, emulsions, syrups, and elixirs. The compound or pharmaceutically acceptable salt of the compound of this invention can be dissolved or suspended in a pharmaceutically acceptable liquid carrier such as water, an organic solvent, a mixture of both, or pharmaceutically acceptable oils or fat. The liquid carrier can contain other suitable pharmaceutical additives including solubilizers, emulsifiers, buffers, preservatives, sweeteners, flavoring agents, suspending agents, thickening agents, colors, viscosity regulators, stabilizers, or osmo-regulators. Suitable examples of liquid carriers for oral and parenteral administration include water (particular containing additives as above, e.g., cellulose derivatives, including sodium carboxymethyl cellulose solution), alcohols (including monohydric alcohols and polyhydric alcohols, e.g., glycols) and their derivatives, and oils (e.g., fractionated coconut oil and arachis oil). For parenteral administration the carrier can also be an oily ester such as ethyl oleate and isopropyl myristate. Sterile liquid carriers are used in sterile liquid form compositions for parenteral administration. The liquid carrier for pressurized compositions can be halogenated hydrocarbon or other pharmaceutically acceptable propellant.


The present compositions can take the form of solutions, suspensions, emulsion, tablets, pills, pellets, capsules, capsules containing liquids, powders, sustained-release formulations, suppositories, emulsions, aerosols, sprays, suspensions, or any other form suitable for use. In one embodiment, the composition is in the form of a capsule. Other examples of suitable physiologically acceptable excipients are described in Remington's Pharmaceutical Sciences 1447-1676 (Alfonso R. Gennaro, ed., 19th ed. 1995).


In one embodiment, the compound or a pharmaceutically acceptable salt of the compound is formulated in accordance with routine procedures as a composition adapted for oral administration to humans. Compositions for oral delivery can be in the form of tablets, lozenges, buccal forms, troches, aqueous or oily suspensions or solutions, granules, powders, emulsions, capsules, syrups, or elixirs for example. Orally administered compositions can contain one or more agents, for example, sweetening agents such as fructose, aspartame or saccharin; flavoring agents such as peppermint, oil of wintergreen, or cherry; coloring agents; and preserving agents, to provide a pharmaceutically palatable preparation. In powders, the carrier can be a finely divided solid, which is an admixture with the finely divided compound or pharmaceutically acceptable salt of the compound. In tablets, the compound or pharmaceutically acceptable salt of the compound is mixed with a carrier having the necessary compression properties in suitable proportions and compacted in the shape and size desired. The powders and tablets can contain up to about 99% of the compound or pharmaceutically acceptable salt of the compound.


Capsules may contain mixtures of the compounds or pharmaceutically acceptable salts of the compounds with inert fillers and/or diluents such as pharmaceutically acceptable starches (e.g., corn, potato, or tapioca starch), sugars, artificial sweetening agents, powdered celluloses (such as crystalline and microcrystalline celluloses), flours, gelatins, gums, etc.


Tablet formulations can be made by conventional compression, wet granulation, or dry granulation methods and utilize pharmaceutically acceptable diluents, binding agents, lubricants, disintegrants, surface modifying agents (including surfactants), suspending or stabilizing agents (including, but not limited to, magnesium stearate, stearic acid, sodium lauryl sulfate, talc, sugars, lactose, dextrin, starch, gelatin, cellulose, methyl cellulose, microcrystalline cellulose, sodium carboxymethyl cellulose, carboxymethylcellulose calcium, polyvinylpyrroldine, alginic acid, acacia gum, xanthan gum, sodium citrate, complex silicates, calcium carbonate, glycine, sucrose, sorbitol, dicalcium phosphate, calcium sulfate, lactose, kaolin, mannitol, sodium chloride, low melting waxes, and ion exchange resins. Surface modifying agents include nonionic and anionic surface modifying agents. Representative examples of surface modifying agents include, but are not limited to, poloxamer 188, benzalkonium chloride, calcium stearate, cetostearl alcohol, cetomacrogol emulsifying wax, sorbitan esters, colloidal silicon dioxide, phosphates, sodium dodecylsulfate, magnesium aluminum silicate, and triethanolamine.


Moreover, when in a tablet or pill form, the compositions can be coated to delay disintegration and absorption in the gastrointestinal tract, thereby providing a sustained action over an extended period of time. Selectively permeable membranes surrounding an osmotically active driving compound or a pharmaceutically acceptable salt of the compound are also suitable for orally administered compositions. In these latter platforms, fluid from the environment surrounding the capsule can be imbibed by the driving compound, which swells to displace the agent or agent composition through an aperture. These delivery platforms can provide an essentially zero order delivery profile as opposed to the spiked profiles of immediate release formulations. A time-delay material such as glycerol monostearate or glycerol stearate can also be used. Oral compositions can include standard excipients such as mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, and magnesium carbonate. In one embodiment the excipients are of pharmaceutical grade.


In another embodiment, the compound or a pharmaceutically acceptable salt of the compound can be formulated for intravenous administration. Typically, compositions for intravenous administration comprise sterile isotonic aqueous buffer. Where necessary, the compositions can also include a solubilizing agent. Compositions for intravenous administration can optionally include a local anesthetic such as lignocaine to lessen pain at the site of the injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water-free concentrate in a hermetically sealed container such as an ampule or sachette indicating the quantity of active agent. Where the compound or a pharmaceutically acceptable salt of the compound is to be administered by infusion, it can be dispensed, for example, with an infusion bottle containing sterile pharmaceutical grade water or saline. Where the compound or a pharmaceutically acceptable salt of the compound is administered by injection, an ampule of sterile water for injection or saline can be provided so that the ingredients can be mixed prior to administration.


In another embodiment, the compound or pharmaceutically acceptable salt of the compound can be administered transdermally through the use of a transdermal patch. Transdermal administrations include administrations across the surface of the body and the inner linings of the bodily passages including epithelial and mucosal tissues. Such administrations can be carried out using the present compounds or pharmaceutically acceptable salts of the compounds, in lotions, creams, foams, patches, suspensions, solutions, and suppositories (e.g., rectal or vaginal).


Transdermal administration can be accomplished through the use of a transdermal patch containing the compound or pharmaceutically acceptable salt of the compound and a carrier that is inert to the compound or pharmaceutically acceptable salt of the compound, is non-toxic to the skin, and allows delivery of the agent for systemic absorption into the blood stream via the skin. The carrier may take any number of forms such as creams or ointments, pastes, gels, or occlusive devices. The creams or ointments may be viscous liquid or semisolid emulsions of either the oil-in-water or water-in-oil type. Pastes comprised of absorptive powders dispersed in petroleum or hydrophilic petroleum containing the active ingredient may also be suitable. A variety of occlusive devices may be used to release the compound or pharmaceutically acceptable salt of the compound into the blood stream, such as a semi-permeable membrane covering a reservoir containing the compound or pharmaceutically acceptable salt of the compound with or without a carrier, or a matrix containing the active ingredient.


The compounds or pharmaceutically acceptable salts of the compounds of the invention may be administered rectally or vaginally in the form of a conventional suppository. Suppository formulations may be made from traditional materials, including cocoa butter, with or without the addition of waxes to alter the suppository's melting point, and glycerin. Water-soluble suppository bases, such as polyethylene glycols of various molecular weights, may also be used.


The compound or a pharmaceutically acceptable salt of the compound can be administered by controlled-release or sustained-release means or by delivery devices that are known to those of ordinary skill in the art. Such dosage forms can be used to provide controlled- or sustained-release of one or more active ingredients using, for example, hydropropylmethyl cellulose, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, liposomes, microspheres, or a combination thereof to provide the desired release profile in varying proportions. Suitable controlled- or sustained-release formulations known to those skilled in the art, including those described herein, can be readily selected for use with the active ingredients of the invention. The invention thus encompasses single unit dosage forms suitable for oral administration such as, but not limited to, tablets, capsules, gelcaps, and caplets that are adapted for controlled- or sustained-release.


In one embodiment a controlled- or sustained-release composition comprises a minimal amount of the compound or a pharmaceutically acceptable salt of the compound to treat or prevent a metalloproteinase-related disorder in a minimal amount of time. Advantages of controlled- or sustained-release compositions include extended activity of the drug, reduced dosage frequency, and increased compliance by the animal being treated. In addition, controlled- or sustained-release compositions can favorably affect the time of onset of action or other characteristics, such as blood levels of the compound or a pharmaceutically acceptable salt of the compound, and can thus reduce the occurrence of adverse side effects.


Controlled- or sustained-release compositions can initially release an amount of the compound or a pharmaceutically acceptable salt of the compound that promptly produces the desired therapeutic or prophylactic effect, and gradually and continually release other amounts of the compound or a pharmaceutically acceptable salt of the compound to maintain this level of therapeutic or prophylactic effect over an extended period of time. To maintain a constant level of the compound or a pharmaceutically acceptable salt of the compound in the body, the compound or a pharmaceutically acceptable salt of the compound can be released from the dosage form at a rate that will replace the amount of the compound or a pharmaceutically acceptable salt of the compound being metabolized and excreted from the body. Controlled- or sustained-release of an active ingredient can be stimulated by various conditions, including but not limited to, changes in pH, changes in temperature, concentration or availability of enzymes, concentration or availability of water, or other physiological conditions or compounds.


In certain embodiments, the present invention is directed to prodrugs of the compounds or pharmaceutically acceptable salts of compounds of the present invention. Various forms of prodrugs are known in the art, for example as discussed in Bundgaard, ed., Design of Prodrugs, Elsevier (1985); Widder et al., ed., Methods in Enzymology, vol. 4, Academic Press (1985); Kgrogsgaard-Larsen et al., ed., “Design and Application of Prodrugs”, Textbook of Drug Design and Development, Chapter 5, 113-191 (1991); Bundgaard et al., Journal of Drug Delivey Reviews 1992, 8, 1-38; Bundgaard et al., J. Pharmaceutical Sciences 1988, 77, 285 et seq.; and Higuchi and Stella, eds., Prodrugs as Drug Delivery Systems, American Chemical Society (1975).


The amount of the compound or a pharmaceutically acceptable salt of the compound is an amount that is effective for treating or preventing a metalloproteinase-related disorder. In addition, in vitro or in vivo assays can optionally be employed to help identify optimal dosage ranges. The precise dose to be employed can also depend on the route of administration, the condition, the seriousness of the condition being treated, as well as various physical factors related to the individual being treated, and can be decided according to the judgment of a health-care practitioner. Equivalent dosages may be administered over various time periods including, but not limited to, about every 2 hours, about every 6 hours, about every 8 hours, about every 12 hours, about every 24 hours, about every 36 hours, about every 48 hours, about every 72 hours, about every week, about every two weeks, about every three weeks, about every month, and about every two months. The number and frequency of dosages corresponding to a completed course of therapy will be determined according to the judgment of a health-care practitioner. The effective dosage amounts described herein refer to total amounts administered; that is, if more than one compound or a pharmaceutically acceptable salt of the compound is administered, the effective dosage amounts correspond to the total amount administered.


The amount of the compound or a pharmaceutically acceptable salt of the compound that is effective for treating or preventing a metalloproteinase-related disorder will typically range from about 0.001 mg/kg to about 250 mg/kg of body weight per day, in one embodiment, from about 1 mg/kg to about 250 mg/kg body weight per day, in another embodiment, from about 1 mg/kg to about 50 mg/kg body weight per day, and in another embodiment, from about 1 mg/kg to about 20 mg/kg of body weight per day.


In one embodiment, the pharmaceutical composition is in unit dosage form, e.g., as a tablet, capsule, powder, solution, suspension, emulsion, granule, or suppository. In such form, the composition is sub-divided in unit dose containing appropriate quantities of the active ingredient; the unit dosage form can be packaged compositions, for example, packeted powders, vials, ampoules, prefilled syringes or sachets containing liquids. The unit dosage form can be, for example, a capsule or tablet itself, or it can be the appropriate number of any such compositions in package form. Such unit dosage form may contain from about 1 mg/kg to about 250 mg/kg, and may be given in a single dose or in two or more divided doses.


The compound or a pharmaceutically acceptable salt of the compound can be assayed in vitro or in vivo for the desired therapeutic or prophylactic activity prior to use in humans. Animal model systems can be used to demonstrate safety and efficacy.


Thus, in one embodiment, the invention provides a composition comprising an effective amount of the compound or a pharmaceutically acceptable salt of the compound of the present invention and a pharmaceutically acceptable carrier.


In another embodiment, the pharmaceutically acceptable carrier is suitable for oral administration and the composition comprises an oral dosage form.


Therapeutic or Prophylactic Uses

In one embodiment, the compounds or pharmaceutically acceptable salts of the compounds of the present invention are useful as metalloproteinase modulators. Accordingly, the compounds and pharmaceutically acceptable salts of the compounds of the present invention are useful for treating an animal with a metalloproteinase-related disorder.


In one embodiment, the invention provides a method for treating a metalloproteinase-related disorder, comprising administering to an animal in need thereof a compound or a pharmaceutically acceptable salt of the compound of Formula (I) or Formula (II) in an amount effective to treat a metalloproteinase-related disorder. In another embodiment, the compound or pharmaceutically acceptable salt of the compound is of the Formula (Ia), (Ib), (Ic), or (Id).


In one embodiment, the metalloproteinase is a matrix metalloproteinase or an aggrecanase.


In another embodiment, the aggrecanase is aggrecanase-1 or aggrecanase-2.


In one embodiment, the metalloproteinase-related disorder is selected from the group consisting of arthritic disorders, osteoarthritis, cancer, rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, atherosclerosis, age-related macular degeneration, myocardial infarction, corneal ulceration and other ocular surface diseases, hepatitis, aortic aneurysms, tendonitis, central nervous system diseases, abnormal wound healing, angiogenesis, restenosis, cirrhosis, multiple sclerosis, glomerulonephritis, graft versus host disease, diabetes, inflammatory bowel disease, shock, invertebral disc degeneration, stroke, osteopenia, and periodontal diseases.


In another embodiment, the metalloproteinase-related disorder is osteoarthritis.


In one embodiment, the present invention is directed to a method for modulating the activity of a metalloproteinase in an animal in need thereof, comprising contacting the metalloproteinase with an effective amount of a compound or pharmaceutically acceptable salt of the compound of Formula (I) or Formula (II). In one embodiment, the method further comprises determining the activity of the metalloproteinase. In one embodiment, the step of determining the activity of the metalloproteinase is performed before the step of contacting the metalloproteinase with the compound or a pharmaceutically acceptable salt of the compound. In another embodiment, the step of determining the activity of the metalloproteinase is performed after the step of contacting the metalloproteinase with the compound or a pharmaceutically acceptable salt of the compound. In another embodiment, the compound or pharmaceutically acceptable salt of the compound is of the Formula (Ia), (Ib), (Ic), or (Id).


The compounds and pharmaceutically acceptable salts of the compounds of Formula (I) or Formula (II) are also useful in the manufacture of medicaments for treating a metalloproteinase-related disorder in an animal. In another embodiment, the compound or pharmaceutically acceptable salt of the compound is of the Formula (Ia), (Ib), (Ic), or (Id).


Accordingly, in one embodiment, the invention provides the use of a compound or pharmaceutically acceptable salt of the compound of Formula (I) or Formula (II) for the manufacture of a medicament for treating a metalloproteinase-related disorder. In another embodiment, the compound or pharmaceutically acceptable salt of the compound is of the Formula (Ia), (Ib), (Ic), or (Id).


In one embodiment, the metalloproteinase is a matrix metalloproteinase or an aggrecanase.


In another embodiment, the aggrecanase is aggrecanase-1 or aggrecanase-2.


In one embodiment, the metalloproteinase-related disorder is selected from the group consisting of arthritic disorders, osteoarthritis, cancer, rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, atherosclerosis, age-related macular degeneration, myocardial infarction, corneal ulceration and other ocular surface diseases, hepatitis, aortic aneurysms, tendonitis, central nervous system diseases, abnormal wound healing, angiogenesis, restenosis, cirrhosis, multiple sclerosis, glomerulonephritis, graft versus host disease, diabetes, inflammatory bowel disease, shock, invertebral disc degeneration, stroke, osteopenia, and periodontal diseases.


In another embodiment, the metalloproteinase-related disorder is osteoarthritis.


In one embodiment, the present invention is directed to the use of a compound or pharmaceutically acceptable salt of the compound of Formula (I) or Formula (II) for the manufacture of a medicament for modulating the activity of a metalloproteinase. In one embodiment, the medicament is also for determining the activity of the receptor. In another embodiment, the compound or pharmaceutically acceptable salt of the compound is of the Formula (Ia), (Ib), (Ic), or (Id).


EXAMPLES






Example 1
N-[4-(pyrimidin-2-ylamino)benzoyl]-L-glutamic acid






Step A: 2-[4-(pyrimidin-2-ylamino)-benzoylamino]-pentanedioic acid diethyl ester

2-Chloropyrimidine (0.72 g, 6.3 mmol) and N-(4-aminobenzoyl)-L-glutamic acid diethyl ester (2.04 g, 6.3 mmol, 1 equiv.) were dissolved in DMF (4 mL) and the resulting mixture was heated to 110° C. for 3 hrs. Reaction was complete as determined by TLC. The mixture was cooled to room temperature and partitioned between ethyl acetate and water. The organic phase was washed with brine, dried over MgSO4, and concentrated by rotavap. The residue was purified by column chromatography (silica gel, 50% EtOAc/hexane) to afford 1.75 g of desired product 2-[4-(pyrimidin-2-ylamino)-benzoylamino]-pentanedioic acid diethyl ester in 69% yield. MS (ESI) m/z 399.


Step B: N-[4-(pyrimidin-2-ylamino)benzoyl]-L-glutamic acid

A solution of 2-[4-(pyrimidin-2-ylamino)-benzoylamino]-pentanedioic acid diethyl ester (250 mg, 0.62 mmol) in THF (4 mL) was added to MeOH (4 mL), followed by the addition of 1N NaOH solution (5 mL, 5 mmol, 8 equiv.). The mixture was stirred at room temperature overnight. Reaction was complete as determined by HPLC. Solvents were removed by rotavap and a small amount of water (5 mL) was added to make a homogeneous solution. 1N HCl was added dropwise until the solution reached a pH of 4. The solid precipitate was collected by filtration, washed with EtOAc, and dried in vacuum oven. The product N-[4-(pyrimidin-2-ylamino)benzoyl]-L-glutamic acid (120 mg) was isolated in 45% yield. MS (ESI) m/z 343.







Example 2
N-(1,1′-biphenyl-4-ylcarbonyl)-D-glutamic acid






Step A: 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid diethyl ester

A solution of 4-biphenylcarboxylic acid (670 mg, 3.4 mmol) and (809 mg, 3.4 mmol, 1 equiv.) in dry DMF (20 mL) was added to EDCI (801 mg, 5.1 mmol, 1.5 equiv.), triethylamine (853 mg, 8.4 mmol, 2.5 equiv.) and DMAP (82 mg, 0.7 mmol, 20%). The mixture was stirred at room temperature overnight. Reaction was complete as determined by TLC. The mixture was diluted with EtOAc and washed with H2O, brine, and dried over magnesium sulfate. Solvent was removed and the residue subject to column chromatography (silica gel, 50% EtOAc/hexane). 660 mg of desired product 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid diethyl ester was isolated in 51% yield. MS (ESI) m/z 382.


Step B: N-(1,1′-biphenyl-4-ylcarbonyl)-D-glutamic acid

A solution of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid diethyl ester (250 mg, 0.65 mmol) in THF (6 mL) was added to MeOH (6 mL), followed by the addition of 1N NaOH solution (5.2 mL, 5.2 mmol, 8 eq.). The mixture was stirred at room temperature overnight. Reaction was complete as determined by HPLC. Solvents were removed by rotavap and a small amount of water (5 mL) was added to make a homogeneous solution. 1N HCl was added dropwise until the solution reached a pH of 4. The solid precipitate was collected by filtration, washed with EtOAc, and dried in vacuum oven. Product N-(1,1′-biphenyl-4-ylcarbonyl)-D-glutamic acid (189 mg) was isolated in 89% yield. MS (ESI) m/z 326.







Example 3
N2-[4-(pyrimidin-2-ylamino)benzoyl]-L-alpha-glutamine
Step A: 4-Carbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino]-butyric acid tert-butyl ester

4-(Pyrimidin-2-ylamino)-benzoic acid (1.80 g, 8.4 mmol, 1 equiv.) and EDCI (2.41 g, 12.6 mmol, 1.5 equiv.) were dissolved in DMF (60 mL) under N2. The mixture was stirred at room temperature for 20 minutes, followed by the addition of H-Glu (OtBu)-NH2 (2 g, 8.4 mmol, 1 equiv), Et3N (2.92 mL, 20.9 mmol, 2.5 equiv.), and DMAP (0.512 g, 4.2 mmol, 0.5 equiv). The mixture was allowed to stir at room temperature overnight. Reaction was complete as determined by TLC. DMF was removed by rotovap and the residue was dissolved in EtOAc. The organic layer was washed with brine and dried over MgSO4. Most of the solvent was removed by rotavap and a solid was precipitated from the mixture during solvent evaporation. The solid was collected by filtration and washed with EtOAc. 823 mg of 4-Carbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino]-butyric acid tert-butyl ester was obtained in 25% yield. MS (ESI) m/z 400.


Step B: N2-[4-(pyrimidin-2-ylamino)benzoyl]-L-alpha-glutamine

4-Carbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino]-butyric acid tert-butyl ester was dissolved in dichloroethane (10 mL), followed by the addition of TFA (5 mL). The mixture was stirred at room temperature for 4 hrs. TLC indicated the reaction was complete. Solvents were then removed by rotavap and the residue was washed with EtOAc. A solid was collected by filtration and dried in vacuum oven overnight. 4-Carbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino]-butyric acid was obtained in quantitative yield (285 mg). MS (ESI) m/z 344.


Example 4
N2-(1,1′-biphenyl-4-ylcarbonyl)-D-alpha-glutamine

The title compound was prepared according to the procedures similar to that described for Example 3. Yield 95%, MS (ESI) m/z 327.







Example 5
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-hydroxybutyl)-L-alpha-glutamine
Step A: 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester

A solution of 4-diphenyl carboxylic acid (3.57 g, 18 mmol, 1.0 equiv.) in DMF (40 mL) was added to EDCI (4.28 g, 22.3 mmol, 1.24 equiv.) and stirred at room temperature for 0.5 hrs. H-Glu(OMe)Ot-Bu (5.02 g, 19.8 mmol, 1.1 equiv.) was added, followed by the addition of Et3N (6.77 mL, 48.6 mmol, 2.7 equiv.) and DMAP (330 mg, 2.7 mmol, 15%). The mixture was stirred overnight under N2. Reaction was complete as determined by TLC. The reaction mixture was diluted with EtOAc, washed with H2O, then brine solution. The organic layer was separated and then dried over MgSO4. After concentration, crude was purified by column chromatography (Silica gel, 20% EtOAc/Hexane) to afford 3.2 g of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester in 44.7% yield. MS (ESI) m/z 396.


Step B: 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester

A solution of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester (1.5 g, 3.8 mmol) in dichloroethane (26 mL) was added to TFA (13 mL) at room temperature. The mixture was stirred for 4 hrs. and TLC indicated the reaction was complete. Solvent was removed by rotovap. The solid thus obtained was dried under vacuum to provide 1.128 g of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester [G9591-161-1] in 87% yield. MS (ESI) m/z 342.


Step C: 4-[(Biphenyl-4-carbonyl)-amino]-4-(4-hydroxy-butylcarbamoyl)-butyric acid methyl ester

A solution of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester (400 mg, 1.2 mmol, 1 equiv.) in DMF (10 mL) was added to EDCI (337 mg, 1.8 mmol, 1.5 equiv.). The solution was stirred at room temperature under N2 for 40 min. 4-Amino-1-butanol (0.11 mL, 1.2 mmol, 1 equiv.) was added, followed by addition of Et3N (0.41 mL, 2.9 mmol, 2.5 equiv.) and DMAP (72 mg, 0.59 mmol, 0.5 equiv.). The mixture was stirred overnight under N2. The reaction mixture was then poured into cold water and a solid precipitated from the solution. The solid was collected by filtration and dried under vacuum to give 229 mg of 4-[(Biphenyl-4-carbonyl)-amino]-4-(4-hydroxy-butylcarbamoyl)-butyric acid methyl ester in 47% yield. MS (ESI) m/z 413.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-hydroxybutyl)-L-alpha-glutamine

A solution of 4-[(Biphenyl-4-carbonyl)-amino]-4-(4-hydroxy-butylcarbamoyl)-butyric acid methyl ester (229 mg, 0.56 mmol, 1 equiv.) in THF (10 mL) and MeOH (2.8 mL) was added to 1N NaOH (2.8 mL, 2.8 mmol, 5 equiv.). The solution was stirred at room temperature for 2.5 hrs. After TLC indicated the reaction was complete, the solvent was evaporated and the residue was dissolved in H2O. After adjusting the pH of the solution to 3 using 1N HCl, a solid precipitated from the solution. The solid was collected by filtration and washed with water. After drying, 120 mg of N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-hydroxybutyl)-L-alpha-glutamine was isolated in 54.1% yield. MS (ESI) m/z 399.


Example 6

The following compounds were prepared according to the procedures similar to those described in Example 5.


Example 6A
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutamine
Step C: methyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutaminate

Described as above according to the procedure in Example 5, except PyBop/DIEA (or Bop/DIEA) was used. MS (ESI) m/z 457.2; MS (ESI) m/z 913.4; HRMS: calcd for C28H28N2O4+H+, 457.21218; found (ESI-FTMS, [M+H]1+), 457.21403.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutamine

MS (ESI) m/z 441.2; MS (ESI) m/z 883.3; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19699.


Example 6B
N2-(biphenyl-4-ylcarbonyl)-N1-(2-hydroxy-1,1-dimethylethyl)-L-α-glutamine
Step C: methyl N2-(biphenyl-4-ylcarbonyl)-N1-(2-hydroxy-1,1-dimethylethyl)-L-α-glutaminate

Described as above according to the procedure in Example 5, except PyBop/DIEA (or Bop/DIEA) was used. MS (ESI) m/z 413.1; MS (ESI) m/z 825.2; HRMS: calcd for C23H28N2O5+H+, 413.20710; found (ESI-FTMS, [M+H]1+), 413.20705.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(2-hydroxy-1,1-dimethylethyl)-L-α-glutamine

MS (ESI) m/z 399.1; MS (ESI) m/z 797.3; HRMS: calcd for C22H26N2O5+H+, 399.19145; found (ESI-FTMS, [M+H]1+), 399.19217.


Example 6C
N2-(biphenyl-4-ylcarbonyl)-N1-(1,1,3,3-tetramethylbutyl)-L-α-glutamine
Step C: methyl N2-(biphenyl-4-ylcarbonyl)-N1-(1,1,3,3-tetramethylbutyl)-L-α-glutaminate

Described as above according to the procedure in Example 5, except PyBop/DIEA (or Bop/DIEA) was used. MS (ESI) m/z 453.2; MS (ESI) m/z 905.4; HRMS: calcd for C27H36N2O4+H+, 453.27478; found (ESI-FTMS, [M+H]1+), 453.27588.


Step D: N2— (biphenyl-4-ylcarbonyl)-N1-(1,1,3,3-tetramethylbutyl)-L-α-glutamine

MS (ESI) m/z 439.2; MS (ESI) m/z 877.4; HRMS: calcd for C26H34N2O4+H+, 439.25913; found (ESI-FTMS, [M+H]1+), 439.25974.


Example 6D
N1-(1,1-dimethyl-2-phenylethyl)-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine
Step A: 1-tert-butyl 5-methyl-N-(9H-fluoren-2-ylcarbonyl)-L-glutamate

Described as above according to the procedure in Example 5, except 2-fluorenecarboxylic acid was used. MS (ESI) m/z 410.2; MS (ESI) m/z 819.4; HRMS: calcd for C24H27NO5+Na+, 432.17814; found (ESI-FTMS, [M+Na]1+), 432.17902.


Step B: (2S)-2-[(9H-fluoren-2-ylcarbonyl)amino]-5-methoxy-5-oxopentanoic acid

MS (ESI) m/z 354.1; MS (ESI) m/z 707.3; HRMS: calcd for C20H19NO5+H+, 354.13360; found (ESI-FTMS, [M+H]1+), 354.13428.


Step C: methyl N1-(1,1-dimethyl-2-phenylethyl)-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutaminate

MS (ESI) m/z 485.3; MS (ESI) m/z 969.5; MS (ESI) m/z 507.3; HRMS: calcd for C30H32N2O4+Na+, 507.22543; found (ESI-FTMS, [M+Na]1+), 507.22863.


Step D: N1-(1,1-dimethyl-2-phenylethyl)-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 471.3; MS (ESI) m/z 941.5.


Example 6E
N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

Step A: described as above according to the procedure in Example 5, except 2-fluorenecarboxylic acid was used.


Step C: methyl N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutaminate

MS (ESI) m/z 519.2; MS (ESI) m/z 1037.5; HRMS: calcd for C30H31ClN2O4+H+, 519.20451; found (ESI-FTMS, [M+H]1+), 519.20406.


Step D: N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 505.2; MS (ESI) m/z 1009.5.







Example 7
N1-(1,3-benzodioxol-5-ylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine
Step A: tert-butyl N1-(1,3-benzodioxol-5-ylmethyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

In a 50 mL round bottom flask, Fmoc-L-Glu-(OtBu)-OH (2.127 g, 5 mmol) was dissolved in DMF (10 mL) and stirred at room temperature under nitrogen. PyBOP (2.6 g, 5 mmol) was added to the stirring mixture followed by piperonylamine (0.755 g, 5 mmol), and DIEA (1.2 equivalent) added dropwise. The mixture was stirred overnight (18-24 hr) at room temperature under nitrogen. The reaction mixture was then poured into 150 mL of water and the solid was collected and washed with water. The wet solid was then dissolved in ethyl acetate (ca. 500 mL), and washed consecutively with 10% HCl (aq.), NaHCO3 (satd.), and NaCl (satd.). The organic phase was dried over MgSO4 (anh.) and concentrated. The crude product was redissolved in acetone and hexanes were added. The precipitated solid was filtered, washed with hexanes and dried in a vacuum desiccator overnight to give 1.56 g of tert-butyl N1-(1,3-benzodioxol-5-ylmethyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate as fine white solid. MS (ESI) m/z 559.1; HRMS: calcd for C32H34N2O7+H+, 559.24388; found (ESI_FT, [M+H]1+), 559.24348.


Step B: tert-butyl N1-(1,3-benzodioxol-5-ylmethyl)-L-α-glutaminate

The Fmoc-ester product (1.5 g, 2.7 mmol) was (partially) dissolved in acetonitrile (17 mL) and stirred at room temperature under nitrogen. The mixture was fully dissolved about 30 min after addition of diethylamine (4.2 mL) and was stirred for two hours. After reaction completion, the mixture is carefully concentrated (<30° C.) under reduced pressure by rotary evaporator. The crude product (clear oil) was then filtered through a short flash column. The column was gradient eluted starting with 5% Acetone/CH2Cl2 until the Fmoc byproduct was removed and ramped to 50% Acetone/CH2Cl2 while the product eluted. The desired fractions were collected and concentrated giving 0.8 g clear oil. MS (ESI) m/z 337.1; HRMS: calcd for C17H24N2O5+H+, 337.17580; found (ESI_FT, [M+H]1+), 337.17605.


Step C: tert-butyl N1-(1,3-benzodioxol-5-ylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutaminate

The free amine (0.36 g, 1.07 mmol) was dissolved in DMF (5 mL) and stirred at rt under nitrogen. 4-Biphenyl carboxylic acid (0.557 g, 1.07 mmol) was added followed by PyBOP reagent (0.21 g, 1.07 mmol) with vigorous stirring. The reaction mixture was cooled to 0° C. and DIEA (1.2 equiv.) was added dropwise. The reaction was complete in 14-24 hours. The reaction mixture was then poured into 100 mL water and the solid collected. The solid was redissolved in ethyl acetate and washed with 10% HCl (aq.), NaHCO3 (satd.), and NaCl (satd.). The organic phase was dried over Na2SO4 (anh.) and concentrated. Acetone was added to the residue and diluted with hexanes. The precipitate was collected and dried in a vacuum desiccator overnight to give 0.4 g of desired product. MS (ESI) m/z 517.2; HRMS: calcd for C30H32N2O6+H+, 517.23331; found (ESI_FT, [M+H]1+), 517.23311.


Step D: N1-(1,3-benzodioxol-5-ylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

The t-butyl ester (0.31 g, 0.6 mmol) was dissolved in CH2Cl2 (4 mL) and stirred at rt under nitrogen. Trifluoroacetic acid (4 mL) dissolved in CH2Cl2 (4 mL) is added to the solution and the light yellow solution is stirred at rt for 2 hours. The mixture is evaporated and diluted toluene (10 mL), evaporated to fully remove the TFA. A solid is obtained and dissolved in minimum acetone (ca. 25-50 mL) warmed slightly and hexanes added. The white solid was collected and dried in a vacuum desiccator to yield 0.214 g product. MS (ESI) m/z 461.1; HRMS: calcd for C26H24N2O6+H+, 461.17071; found (ESI_FT, [M+H]1+), 461.1704.


Example 8

The following compounds were prepared according to the procedures similar to those of Example 7.


Example 8A
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclohexyl-L-α-glutamine
Step A: tert-butyl N1-cyclohexyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 507.2; HRMS: calcd for C30H38N2O5+H+, 507.28535; found (ESI_FT, [M+H]1+), 507.28583.


Step B: tert-butyl N1-cyclohexyl-L-α-glutaminate

MS (ESI) m/z 285.2; HRMS: calcd for C15H28N2O3+H+, 285.21727; found (ESI_FT, [M+H]1+), 285.21644.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclohexyl-L-α-glutaminate

MS (ESI) m/z 465.2; HRMS: calcd for C28H36N2O4+H+, 465.27478; found (ESI_FT, [M+H]1+), 465.27386.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclohexyl-L-α-glutamine

HRMS: calcd for C24H28N2O4+H+, 409.21218; found (ESI_FT, [M+H]1+), 409.21163.


Example 8B
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopropyl-L-α-glutamine
Step A: tert-butyl N1-cyclopropyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 465.1; HRMS: calcd for C27H32N2O5+H+, 465.23840; found (ESI_FT, [M+H]1+), 465.23816.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopropyl-L-α-glutaminate

MS (ESI) m/z 423.2; HRMS: calcd for C25H30N2O4+H+, 423.22783; found (ESI_FT, [M+H]1+), 423.22725.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopropyl-L-α-glutamine

MS (ESI) m/z 367.1; MS (ESI) m/z 733.2; HRMS: calcd for C21H22N2O4+H+, 367.16523; found (ESI_FT, [M+H]1+), 367.16454.


Example 8C
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-butyl-L-α-glutamine
Step A: tert-butyl N1-butyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 481.2; MS (ESI) m/z 503.2; HRMS: calcd for C28H36N2O5+H+, 481.26970; found (ESI_FT, [M+H]1+), 481.26789.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-butyl-L-α-glutaminate

MS (ESI) m/z 439.1; MS (ESI) m/z 877.3; HRMS: calcd for C26H34N2O4+H+, 439.25913; found (ESI_FT, [M+H]1+), 439.25884.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-butyl-L-α-glutamine

MS (ESI) m/z 381.2; MS (ESI) m/z 763.3; HRMS: calcd for C22H26N2O4+H+, 383.19653; found (ESI_FT, [M+H]1+), 383.19514.


Example 8D
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-fluorobenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 533.2; HRMS: calcd for C31H33FN2O5+H+, 533.24463; found (ESI_FT, [M+H]1+), 533.24546.


Step B: tert-butyl N1-(4-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 311.1; HRMS: calcd for C16H23FN2O3+H+, 311.17655; found (ESI_FT, [M+H]1+), 311.17696.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 491.2; HRMS: calcd for C29H31FN2O4+H+, 491.23406; found (ESI_FT, [M+H]1+), 491.23339.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-fluorobenzyl)-L-α-glutamine

MS (ESI) m/z 435.1; HRMS: calcd for C25H23FN2O4+H+, 435.17146; found (ESI_FT, [M+H]1+), 435.17056.


Example 8E
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclooctyl-L-α-glutamine
Step A: tert-butyl N1-cyclooctyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 535.2; HRMS: calcd for C32H42N2O5+H+, 535.31665; found (ESI_FT, [M+H]1+), 535.31782.


Step B: tert-butyl N1-cyclooctyl-L-α-glutaminate

MS (ESI_FT) m/z 313.24821; MS (ESI_FT) m/z 313.24857; HRMS: calcd for C17H32N2O3+H+, 313.24857; found (ESI_FT, [M+H]1+), 313.24821.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclooctyl-L-α-glutaminate

MS (ESI) m/z 493.4; MS (ESI) m/z 985.6; HRMS: calcd for C30H40N2O4+H+, 493.30608; found (ESI_FTMS, [M+H]1+), 493.30412.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclooctyl-L-α-glutamine

MS (ESI) m/z 437.3; MS (ESI) m/z 873.6; HRMS: calcd for C26H32N2O4+H+, 437.24348; found (ESI_FT, [M+H]1+), 437.24272.


Example 8F
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethoxybenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,4-dimethoxybenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 575.3; MS (ESI) m/z 1149.6; HRMS: calcd for C33H38N2O7+H+, 575.27518; found (ESI_FT, [M+H]1+), 575.27609.


Step B: tert-butyl N1-(3,4-dimethoxybenzyl)-L-α-glutaminate

MS (ESI+) m/z 353.2; MS (ESI+) m/z 297.1; HRMS: calcd for C18H28N2O5+H+, 353.20710; found (ESI_FT, [M+H]1+), 353.20629.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethoxybenzyl)-L-α-glutaminate

MS (ESI+) m/z 533.3; HRMS: calcd for C31H36N2O6+H+, 533.26461; found (ESI_FT, [M+H]1+), 533.26323.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethoxybenzyl)-L-α-glutamine

MS (ESI−) m/z 475.3; HRMS: calcd for C27H28N2O6+H+, 477.20201; found (ESI_FT, [M+H]1+), 477.20053.


Example 8G
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopentyl-L-α-glutamine
Step A: tert-butyl N1-cyclopentyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI+) m/z 493.3; MS (ESI+) m/z 437.2; HRMS: calcd for C29H36N2O5+H+, 493.26970; found (ESI_FT, [M+H]1+), 493.26812.


Step B: tert-butyl N1-cyclopentyl-L-α-glutaminate

MS (ESI) m/z 271.2; HRMS: calcd for C14H26N2O3+H+, 271.20162; found (ESI_FT, [M+H]1+), 271.202.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopentyl-L-α-glutaminate

MS (ESI) m/z 451.3; MS (ESI) m/z 901.6; HRMS: calcd for C27H34N2O4+H+, 451.25913; found (ESI_FT, [M+H]1+), 451.25803.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-cyclopentyl-L-α-glutamine

MS (ESI) m/z 395.2; MS (ESI) m/z 789.4; HRMS: calcd for C23H26N2O4+H+, 395.19653; found (ESI_FT, [M+H]1+), 395.19564.


Example 8H
N1-2-adamantyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine
Step A: tert-butyl N1-2-adamantyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 559.4; MS (ESI) m/z 1117.7; HRMS: calcd for C34H42N2O5+H+, 559.31665; found (ESI_FT, [M+H]1+), 559.31524.


Step B: tert-butyl N1-2-adamantyl-L-α-glutaminate

MS (ESI) m/z 337.2; MS (ESI) m/z 673.5; HRMS: calcd for C19H32N2O3+H+, 337.24857; found (ESI_FT, [M+H]1+), 337.2475.


Step C: tert-butyl N1-2-adamantyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutaminate

MS (ESI) m/z 517.3; MS (ESI) m/z 1033.6; HRMS: calcd for C32H40N2O4+H+, 517.30608; found (ESI_FT, [M+H]1+), 517.30505.


Step D: N1-2-adamantyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 461.3; MS (ESI) m/z 921.5; HRMS: calcd for C28H32N2O4+H+, 461.24348; found (ESI_FT, [M+H]1+), 461.24227.


Example 8I
N1-(2-adamantylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine
Step A: tert-butyl N1-(2-adamantylmethyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 573.3; MS (ESI) m/z 1145.5; HRMS: calcd for C35H44N2O5+H+, 573.33230; found (ESI_FT, [M+H]1+), 573.33089.


Step B: tert-butyl N1-(2-adamantylmethyl)-L-α-glutaminate

MS (ESI) m/z 351.2; HRMS: calcd for C20H34N2O3+H+, 351.26422; found (ESI_FT, [M+H]1+), 351.26491.


Step C: tert-butyl N1-(2-adamantylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutaminate

MS (ESI) m/z 531.3; MS (ESI) m/z 1061.4; HRMS: calcd for C33H42N2O4+H+, 531.32173; found (ESI_FT, [M+H]1+), 531.32031.


Step D: N1-(2-adamantylmethyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 475.2; MS (ESI) m/z 949.3; HRMS: calcd for C29H34N2O4+H+, 475.25913; found (ESI_FT, [M+H]1+), 475.25734.


Example 8J
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxyethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-methoxyethyl)-L-α-glutaminate

MS (ESI) m/z 483.2; MS (ESI) m/z 965.3; HRMS: calcd for C27H34N2O6+H+, 483.24896; found (ESI_FT, [M+H]1+), 483.24851.


Step B: tert-butyl N1-(2-methoxyethyl)-L-α-glutaminate

MS (ESI) m/z 261.1; HRMS: calcd for C12H24N2O4+H+, 261.18088; found (ESI_FT, [M+H]1+), 261.18071.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxyethyl)-L-α-glutaminate

MS (ESI) m/z 441.2; MS (ESI) m/z 881.3; HRMS: calcd for C25H32N2O5+H+, 441.23840; found (ESI_FT, [M+H]1+), 441.23803.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxyethyl)-L-α-glutamine

MS (ESI) m/z 385.1; MS (ESI) m/z 769.2; HRMS: calcd for C21H24N2O5+H+, 385.17580; found (ESI_FT, [M+H]1+), 385.17538.


Example 8K
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxybenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 545.2; MS (ESI) m/z 1089.3; HRMS: calcd for C32H36N2O6+H+, 545.26461; found (ESI_FT, [M+H]1+), 545.26277.


Step B: tert-butyl N1-(4-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 323.2; HRMS: calcd for C17H26N2O4+H+, 323.19653; found (ESI_FT, [M+H]1+), 323.19702.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.4; HRMS: calcd for C30H34N2O5+H+, 503.25405; found (ESI_FT, [M+H]1+), 503.25435.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxybenzyl)-L-α-glutamine

MS (ESI) m/z 445.2; MS (ESI) m/z 891.5; HRMS: calcd for C26H26N2O5+H+, 447.19145; found (ESI_FT, [M+H]1+), 447.19235.


Example 8L
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3,4-dimethoxyphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(3,4-dimethoxyphenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 589.2; MS (ESI) m/z 1177.3; HRMS: calcd for C34H40N2O7+H+, 589.29083; found (ESI_FT, [M+H]1+), 589.28932.


Step B: tert-butyl N1-[2-(3,4-dimethoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 367.2; HRMS: calcd for C19H30N2O5+H+, 367.22275; found (ESI_FT, [M+H]1+), 367.22308.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3,4-dimethoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 547.3; MS (ESI) m/z 1093.4; HRMS: calcd for C32H38N2O6+H+, 547.28026; found (ESI_FT, [M+H]1+), 547.280241.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3,4-dimethoxyphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 491.2; MS (ESI) m/z 981.2; HRMS: calcd for C28H30N2O6+H+, 491.21766; found (ESI_FT, [M+H]1+), 491.21697.


Example 8M
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 559.2; MS (ESI) m/z 1117.3; HRMS: calcd for C33H38N2O6+H+, 559.28026; found (ESI_FT, [M+H]1+), 559.27899.


Step B: tert-butyl N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 337.1; MS (ESI) m/z 673.3; HRMS: calcd for C18H28N2O4+H+, 337.21218; found (ESI_FT, [M+H]1+), 337.21196.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 517.2; MS (ESI) m/z 1033.4; HRMS: calcd for C31H36N2O5+H+, 517.26970; found (ESI_FT, [M+H]1+), 517.26875.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-methoxyphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 461.2; MS (ESI) m/z 921.3; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FT, [M+H]1+), 461.2062.


Example 8N
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(1-naphthylmethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(1-naphthylmethyl)-L-α-glutaminate

MS (ESI) m/z 565.2; MS (ESI) m/z 1129.4; HRMS: calcd for C35H36N2O5+H+, 565.26970; found (ESI_FT, [M+H]1+), 565.26849.


Step B: tert-butyl N1-(1-naphthylmethyl)-L-α-glutaminate

MS (ESI) m/z 343.1; MS (ESI) m/z 685.3; HRMS: calcd for C20H26N2O3+H+, 343.20162; found (ESI_FT, [M+H]1+), 343.2014.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(1-naphthylmethyl)-L-α-glutaminate

MS (ESI) m/z 523.2; MS (ESI) m/z 1045.4; HRMS: calcd for C33H34N2O4+H+, 523.25913; found (ESI_FT, [M+H]1+), 523.2583.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(1-naphthylmethyl)-L-α-glutamine

MS (ESI) m/z 467.2; MS (ESI) m/z 933.3; HRMS: calcd for C29H26N2O4+H+, 467.19653; found (ESI_FT, [M+H]1+), 467.19528.


Example 8O
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 529.1; MS (ESI) m/z 1057.4; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FT, [M+H]1+), 529.26804.


Step B: tert-butyl N1-(3-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 307.1; MS (ESI) m/z 613.3; HRMS: calcd for C17H26N2O3+H+, 307.20162; found (ESI_FT, [M+H]1+), 307.20079.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutaminate

HRMS: calcd for C30H34N2O4+H+, 487.25913; found (ESI_FT, [M+H]1+), 487.25842.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine

HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI_FT, [M+H]1+), 431.19568.


Example 8P
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-furylmethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-furylmethyl)-L-α-glutaminate

MS (ESI) m/z 505.2; HRMS: calcd for C29H32N2O6+H+, 505.23331; found (ESI_FT, [M+H]1+), 505.23263.


Step B: tert-butyl N1-(2-furylmethyl)-L-α-glutaminate

HRMS: calcd for C14H22N2O4+H+, 283.16523; found (ESI_FT, [M+H]1+), 283.16497.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-furylmethyl)-L-α-glutaminate

HRMS: calcd for C27H30N2O5+H+, 463.22275; found (ESI_FT, [M+H]1+), 463.22212.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-furylmethyl)-L-α-glutamine

HRMS: calcd for C23H22N2O5+H+, 407.16015; found (ESI_FT, [M+H]1+), 407.15957.


Example 8Q
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methoxybenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 545.3; MS (ESI) m/z 1089.5; HRMS: calcd for C32H36N2O6+H+, 545.26461; found (ESI_FT, [M+H]1+), 545.26286.


Step B: tert-butyl N1-(3-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 323.3; MS (ESI) m/z 645.7; HRMS: calcd for C17H26N2O4+H+, 323.19653; found (ESI_FT, [M+H]1+), 323.19687.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.5; HRMS: calcd for C30H34N2O5+H+, 503.25405; found (ESI_FT, [M+H]1+), 503.25322.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-methoxybenzyl)-L-α-glutamine

MS (ESI) m/z 447.2; MS (ESI) m/z 893.3; HRMS: calcd for C26H26N2O5+H+, 447.19145; found (ESI_FT, [M+H]1+), 447.19079.


Example 8R
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 605.2; MS (ESI) m/z 1209.4; HRMS: calcd for C34H40N2O8+H+, 605.28574; found (ESI_FT, [M+H]1+), 605.28453.


Step B: tert-butyl N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 383.1; MS (ESI) m/z 765.3; HRMS: calcd for C19H30N2O6+H+, 383.21766; found (ESI_FT, [M+H]1+), 383.21755.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 563.3; MS (ESI) m/z 1125.5; HRMS: calcd for C32H38N2O7+H+, 563.27518; found (ESI_FT, [M+H]1+), 563.2744.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 507.2; MS (ESI) m/z 1013.4; HRMS: calcd for C28H30N2O7+H+, 507.21258; found (ESI_FT, [M+H]1+), 507.21179.


Example 8S
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dichlorobenzyl)-L-α-glutamine
Step A: tert-butyl N1-(2,4-dichlorobenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 583.1; HRMS: calcd for C31H32Cl2N2O5+H+, 583.17610; found (ESI_FT, [M+H]1+), 583.1742.


Step B: tert-butyl N1-(2,4-dichlorobenzyl)-L-α-glutaminate

MS (ESI) m/z 361.1; MS (ESI) m/z 721.2; HRMS: calcd for C16H22Cl2N2O3+H+, 361.10802; found (ESI_FT, [M+H]1+), 361.10891.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dichlorobenzyl)-L-α-glutaminate

MS (ESI) m/z 541.2; MS (ESI) m/z 1081.3; HRMS: calcd for C29H30Cl2N2O4+H+, 541.16554; found (ESI_FT, [M+H]1+), 541.16693.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dichlorobenzyl)-L-α-glutamine

MS (ESI) m/z 483.1; MS (ESI) m/z 967.2; HRMS: calcd for C25H22Cl2N2O4+H+, 485.10294; found (ESI_FT, [M+H]1+), 485.10231.


Example 8T
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(trifluoromethoxy)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[4-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 599.1; MS (ESI) m/z 1197.3; HRMS: calcd for C32H33F3N2O6+H+, 599.23635; found (ESI_FT, [M+H]1+), 599.23521.


Step B: tert-butyl N1-[4-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 377.1; HRMS: calcd for C17H23F3N2O4+H+, 377.16827; found (ESI_FT, [M+H]1+), 377.16901.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 557.2; MS (ESI) m/z 1113.4; HRMS: calcd for C30H31F3N2O5+H+, 557.22578; found (ESI_FT, [M+H]1+), 557.22537.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(trifluoromethoxy)benzyl]-L-α-glutamine

MS (ESI) m/z 501.2; MS (ESI) m/z 1001.3; HRMS: calcd for C26H23F3N2O5+H+, 501.16318; found (ESI_FT, [M+H]1+), 501.16263.


Example 8U
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 599.2; MS (ESI) m/z 1197.4; HRMS: calcd for C32H33F3N2O6+H+, 599.23635; found (ESI_FT, [M+H]1+), 599.23596.


Step B: tert-butyl N1-[3-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 377.1; MS (ESI) m/z 753.3; HRMS: calcd for C17H23F3N2O4+H+, 377.16827; found (ESI_FT, [M+H]1+), 377.1691.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutaminate

MS (ESI) m/z 557.2; MS (ESI) m/z 1113.4; HRMS: calcd for C30H31F3N2O5+H+, 557.22578; found (ESI_FT, [M+H]1+), 557.22558.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutamine

MS (ESI) m/z 499.1; MS (ESI) m/z 999.3; HRMS: calcd for C26H23F3N2O5+H+, 501.16318; found (ESI_FT, [M+H]1+), 501.16265.


Example 8V
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(methylthio)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[4-(methylthio)benzyl]-L-α-glutaminate

MS (ESI) m/z 561.2; MS (ESI) m/z 1121.4; HRMS: calcd for C32H36N2O5S+H+, 561.24177; found (ESI_FT, [M+H]1+), 561.24183.


Step B: tert-butyl N1-[4-(methylthio)benzyl]-L-α-glutaminate

MS (ESI) m/z 339.2; MS (ESI) m/z 677.3; HRMS: calcd for C17H26N2O3S+H+, 339.17369; found (ESI_FT, [M+H]1+), 339.1748.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(methylthio)benzyl]-L-α-glutaminate

MS (ESI) m/z 519.2; MS (ESI) m/z 1037.4; HRMS: calcd for C30H34N2O4S+H+, 519.23120; found (ESI_FT, [M+H]1+), 519.23078.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-(methylthio)benzyl]-L-α-glutamine

MS (ESI) m/z 461.1; MS (ESI) m/z 923.3; HRMS: calcd for C26H26N2O4S+H+, 463.16860; found (ESI_FT, [M+H]1+), 463.16807.


Example 8W
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenoxybenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-phenoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 607.3; MS (ESI) m/z 1213.5; HRMS: calcd for C37H38N2O6+H+, 607.28026; found (ESI_FT, [M+H]1+), 607.27904.


Step B: tert-butyl N1-(4-phenoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 385.2; MS (ESI) m/z 769.4; HRMS: calcd for C22H28N2O4+H+, 385.21218; found (ESI_FT, [M+H]1+), 385.21141.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 565.3; MS (ESI) m/z 1129.5; HRMS: calcd for C35H36N2O5+H+, 565.26970; found (ESI_FT, [M+H]1+), 565.27062.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenoxybenzyl)-L-α-glutamine

MS (ESI) m/z 507.2; MS (ESI) m/z 1015.4; HRMS: calcd for C31H28N2O5+H+, 509.20710; found (ESI_FT, [M+H]1+), 509.20657.


Example 8X
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 559.3; MS (ESI) m/z 1117.6; HRMS: calcd for C33H38N2O6+H+, 559.28026; found (ESI_FT, [M+H]1+), 559.28074.


Step B: tert-butyl N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 337.2; HRMS: calcd for C18H28N2O4+H+, 337.21218; found (ESI_FT, [M+H]1+), 337.21275.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 517.3; MS (ESI) m/z 1033.6; HRMS: calcd for C31H36N2O5+H+, 517.26970; found (ESI_FT, [M+H]1+), 517.27068.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 461.2; MS (ESI) m/z 921.4; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FT, [M+H]1+), 461.20723.


Example 8Y
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dimethoxybenzyl)-L-α-glutamine
Step A: tert-butyl N1-(2,4-dimethoxybenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 575.3; MS (ESI) m/z 1149.5; HRMS: calcd for C33H38N2O7+H+, 575.27518; found (ESI_FT, [M+H]1+), 575.275181.


Step B: tert-butyl N1-(2,4-dimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 353.2; MS (ESI) m/z 705.4; HRMS: calcd for C18H28N2O5+H+, 353.20710; found (ESI_FT, [M+H]1+), 353.20767.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 533.3; MS (ESI) m/z 1065.6; HRMS: calcd for C31H36N2O6+H+, 533.26461; found (ESI_FT, [M+H]1+), 533.26569.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,4-dimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 477.2; MS (ESI) m/z 953.4; HRMS: calcd for C27H28N2O6+H+, 477.20201; found (ESI_FT, [M+H]1+), 477.2019.


Example 8Z
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxybenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 545.2; HRMS: calcd for C32H36N2O6+H+, 545.26461; found (ESI_FT, [M+H]1+), 545.26356.


Step B: tert-butyl N1-(2-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 323.2; MS (ESI) m/z 645.4; HRMS: calcd for C17H26N2O4+H+, 323.19653; found (ESI_FT, [M+H]1+), 323.19657.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.5; HRMS: calcd for C30H34N2O5+H+, 503.25405; found (ESI_FT, [M+H]1+), 503.2546.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methoxybenzyl)-L-α-glutamine

MS (ESI) m/z 447.2; MS (ESI) m/z 893.4; HRMS: calcd for C26H26N2O5+H+, 447.19145; found (ESI_FT, [M+H]1+), 447.19143.


Example 8AA
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-difluorobenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,5-difluorobenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 551.2; MS (ESI) m/z 1101.4; HRMS: calcd for C31H32F2N2O5+H+, 551.23521; found (ESI_FT, [M+H]1+), 551.23338.


Step B: tert-butyl N1-(3,5-difluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 329.1; MS (ESI) m/z 657.3; HRMS: calcd for C16H22F2N2O3+H+, 329.16712; found (ESI_FT, [M+H]1+), 329.16762.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-difluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 509.2; MS (ESI) m/z 1017.3; HRMS: calcd for C29H30F2N2O4+H+, 509.22464; found (ESI_FTMS, [M+H]1+), 509.22463.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-difluorobenzyl)-L-α-glutamine

MS (ESI) m/z 451.1; MS (ESI) m/z 903.3; HRMS: calcd for C25H22F2N2O4+H+, 453.16204; found (ESI_FTMS, [M+H]1+), 453.16222.


Example 8BB
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dichlorobenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,4-dichlorobenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 583.1; MS (ESI) m/z 1165.2; HRMS: calcd for C31H32Cl2N2O5+H+, 583.17610; found (ESI_FT, [M+H]1+), 583.17528.


Step B: tert-butyl N1-(3,4-dichlorobenzyl)-L-α-glutaminate

MS (ESI) m/z 361.1; MS (ESI) m/z 721.2; HRMS: calcd for C16H22Cl2N2O3+H+, 361.10802; found (ESI_FT, [M+H]1+), 361.10861.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dichlorobenzyl)-L-α-glutaminate

MS (ESI) m/z 541.2; MS (ESI) m/z 1081.3; HRMS: calcd for C29H30Cl2N2O4+H+, 541.16554; found (ESI_FT, [M+H]1+), 541.16685.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dichlorobenzyl)-L-α-glutamine

MS (ESI) m/z 483.1; MS (ESI) m/z 967.2; HRMS: calcd for C25H22Cl2N2O4+H+, 485.10294; found (ESI_FTMS, [M+H]1+), 485.10288.


Example 8CC
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-dimethoxybenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,5-dimethoxybenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 575.3; HRMS: calcd for C33H38N2O7+H+, 575.27518; found (ESI_FTMS, [M+H]1+), 575.27468.


Step B: tert-butyl N1-(3,5-dimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 353.2; HRMS: calcd for C18H28N2O5+H+, 353.20710; found (ESI_FTMS, [M+H]1+), 353.20732.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-dimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 533.3; MS (ESI) m/z 1065.5; HRMS: calcd for C31H36N2O6+H+, 533.26461; found (ESI_FTMS, [M+H]1+), 533.26474.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,5-dimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 477.2; MS (ESI) m/z 953.4; HRMS: calcd for C27H28N2O6+H+, 477.20201; found (ESI-FTMS, [M+H]1+), 477.202.


Example 8DD
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,4-dimethylbenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 543.3; MS (ESI) m/z 1085.4; HRMS: calcd for C33H38N2O5+H+, 543.28535; found (ESI_FTMS, [M+H]1+), 543.28604.


Step B: tert-butyl N1-(3,4-dimethylbenzyl)-L-α-glutaminate

MS (ESI) m/z 321.2; HRMS: calcd for C18H28N2O3+H+, 321.21727; found (ESI_FTMS, [M+H]1+), 321.21716.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-L-α-glutaminate

MS (ESI) m/z 501.3; MS (ESI) m/z 1001.4; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FTMS, [M+H]1+), 501.2735.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-L-α-glutamine

MS (ESI) m/z 445.2; MS (ESI) m/z 889.3; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FTMS, [M+H]1+), 445.21199.


Example 8EE
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.4; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FTMS, [M+H]1+), 529.27003.


Step B: tert-butyl N1-(2-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 307.2; MS (ESI) m/z 613.4; HRMS: calcd for C17H26N2O3+H+, 307.20162; found (ESI_FTMS, [M+H]1+), 307.20094.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 487.2; MS (ESI) m/z 973.3; HRMS: calcd for C30H34N2O4+H+, 487.25913; found (ESI_FTMS, [M+H]1+), 487.2588.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 431.2; MS (ESI) m/z 861.3; HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI_FTMS, [M+H]1+), 431.1966.


Example 8FF
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-phenylpropyl)-L-α-glutaminate

MS (ESI) m/z 543.3; HRMS: calcd for C33H38N2O5+H+, 543.28535; found (ESI_FTMS, [M+H]1+), 543.2847.


Step B: tert-butyl N1-(3-phenylpropyl)-L-α-glutaminate

MS (ESI) m/z 321.2; MS (ESI) m/z 641.4; HRMS: calcd for C18H28N2O3+H+, 321.21727; found (ESI_FTMS, [M+H]1+), 321.2167.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutaminate

MS (ESI) m/z 501.3; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FTMS, [M+H]1+), 501.2742.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine

MS (ESI) m/z 445.2; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FTMS, [M+H]1+), 445.2121.


Example 8GG
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutamine
Step A: tert-butyl N1-2,3-dihydro-1H-inden-2-yl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 541.2; MS (ESI) m/z 1081.4; HRMS: calcd for C33H36N2O5+H+, 541.26970; found (ESI-FTMS, [M+H]1+), 541.27088.


Step B: tert-butyl N1-2,3-dihydro-1H-inden-2-yl-L-α-glutaminate

MS (ESI) m/z 319.1; MS (ESI) m/z 637.3; HRMS: calcd for C18H26N2O3+H+, 319.20162; found (ESI-FTMS, [M+H]1+), 319.20275.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutaminate

MS (ESI) m/z 499.2; MS (ESI) m/z 997.4; HRMS: calcd for C31H34N2O4+H+, 499.25913; found (ESI-FTMS, [M+H]1+), 499.258.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-2,3-dihydro-1H-inden-2-yl-L-α-glutamine

MS (ESI) m/z 441.2; MS (ESI) m/z 883.3; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19699.


Example 8HH
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,3-dimethylbenzyl)-L-α-glutamine
Step A: tert-butyl N1-(2,3-dimethylbenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 543.2; MS (ESI) m/z 1085.4; HRMS: calcd for C33H38N2O5+H+, 543.28535; found (ESI-FTMS, [M+H]1+), 543.28623.


Step B: tert-butyl N1-(2,3-dimethylbenzyl)-L-α-glutaminate

MS (ESI) m/z 321.2; MS (ESI) m/z 641.4; HRMS: calcd for C18H28N2O3+H+, 321.21727; found (ESI-FTMS, [M+H]1+), 321.2186.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,3-dimethylbenzyl)-L-α-glutaminate

MS (ESI) m/z 501.2; MS (ESI) m/z 1001.4; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI-FTMS, [M+H]1+), 501.27629.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2,3-dimethylbenzyl)-L-α-glutamine

MS (ESI) m/z 443.2; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI-FTMS, [M+H]1+), 445.21354.


Example 8II
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenylbutyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-phenylbutyl)-L-α-glutaminate

MS (ESI) m/z 557.2; MS (ESI) m/z 1113.4; HRMS: calcd for C34H40N2O5+H+, 557.30100; found (ESI-FTMS, [M+H]1+), 557.30162.


Step B: tert-butyl N1-(4-phenylbutyl)-L-α-glutaminate

MS (ESI) m/z 335.2; HRMS: calcd for C19H30N2O3+H+, 335.23292; found (ESI-FTMS, [M+H]1+), 335.23453;


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenylbutyl)-L-α-glutaminate

MS (ESI) m/z 515.3; MS (ESI) m/z 1029.5; HRMS: calcd for C32H38N2O4+H+, 515.29043; found (ESI-FTMS, [M+H]1+), 515.29152.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-phenylbutyl)-L-α-glutamine

MS (ESI) m/z 459.2; MS (ESI) m/z 917.4; HRMS: calcd for C28H30N2O4+H+, 459.22783; found (ESI-FTMS, [M+H]1+), 459.22972.


Example 8JJ
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 641.1; MS (ESI) m/z 1281.1; HRMS: calcd for C31H33IN2O5+H+, 641.15070; found (ESI-FTMS, [M+H]1+), 641.15111.


Step B: tert-butyl N1-(3-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 419.1; MS (ESI) m/z 837.1; HRMS: calcd for C16H23IN2O3+H+, 419.08262; found (ESI-FTMS, [M+H]1+), 419.08459.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 599.1; MS (ESI) m/z 1197.1; HRMS: calcd for C29H31IN2O4+H+, 599.14013; found (ESI-FTMS, [M+H]1+), 599.1414.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutamine

MS (ESI) m/z 543.1; MS (ESI) m/z 1085.1; MS (ESI) m/z 601.1; HRMS: calcd for C25H23IN2O4+H+, 543.07753; found (ESI-FTMS, [M+H]1+), 543.07723.


Example 8KK
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-tert-butylbenzyl)-L-α-glutamine
Step A: tert-butyl N1-(4-tert-butylbenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 571.3; MS (ESI) m/z 1141.6; HRMS: calcd for C35H42N2O5+H+, 571.31665; found (ESI_FT, [M+H]1+), 571.31581.


Step B: tert-butyl N1-(4-tert-butylbenzyl)-L-α-glutaminate

MS (ESI) m/z 349.2; HRMS: calcd for C20H32N2O3+H+, 349.24857; found (ESI-FTMS, [M+H]1+), 349.2489.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-tert-butylbenzyl)-L-α-glutaminate

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.4; HRMS: calcd for C33H40N2O4+H+, 529.30608; found (ESI-FTMS, [M+H]1+), 529.30622.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-tert-butylbenzyl)-L-α-glutamine

MS (ESI) m/z 473.2; MS (ESI) m/z 945.3; MS (ESI) m/z 531.2; HRMS: calcd for C29H32N2O4+H+, 473.24348; found (ESI-FTMS, [M+H]1+), 473.24348.


Example 8LL
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-iodobenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 641.1; MS (ESI) m/z 1281.1; HRMS: calcd for C31H33IN2O5+H+, 641.15070; found (ESI-FTMS, [M+H]1+), 641.15129.


Step B: tert-butyl N1-(4-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 419; HRMS: calcd for C16H23IN2O3+H+, 419.08262; found (ESI-FTMS, [M+H]1+), 419.08332.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-iodobenzyl)-L-α-glutaminate

MS (ESI) m/z 599; MS (ESI) m/z 1197; HRMS: calcd for C29H31IN2O4+H+, 599.14013; found (ESI-FTMS, [M+H]1+), 599.1407.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-iodobenzyl)-L-α-glutamine

MS (ESI) m/z 543; MS (ESI) m/z 1085; HRMS: calcd for C25H23IN2O4+H+, 543.07753; found (ESI-FTMS, [M+H]1+), 543.07755.


Example 8MM
N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethylpropyl)-L-α-glutamine
Step A: tert-butyl N1-(1,1-dimethylpropyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 495.3; HRMS: calcd for C29H38N2O5+H+, 495.28535; found (ESI_FT, [M+H]1+), 495.28577.


Step B: tert-butyl N1-(1,1-dimethylpropyl)-L-α-glutaminate

MS (ESI) m/z 273.2; HRMS: calcd for C14H28N2O3+H+, 273.21727; found (ESI-FTMS, [M+H]1+), 273.21738.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethylpropyl)-L-α-glutaminate

MS (ESI) m/z 453.2; MS (ESI) m/z 905.5; HRMS: calcd for C27H36N2O4+H+, 453.27478; found (ESI-FTMS, [M+H]1+), 453.27627.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethylpropyl)-L-α-glutamine

MS (ESI) m/z 397.2; MS (ESI) m/z 793.4; HRMS: calcd for C23H28N2O4+H+, 397.21218; found (ESI-FTMS, [M+H]1+), 397.2139.


Example 8NN
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 591.1; MS (ESI) m/z 1181.2; HRMS: calcd for C34H39ClN2O5+H+, 591.26203; found (ESI-FTMS, [M+H]1+), 591.26263.


Step B: tert-butyl N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutaminate

MS (ESI) m/z 369.1; MS (ESI) m/z 737.2; HRMS: calcd for C19H29ClN2O3+H+, 369.19395; found (ESI-FTMS, [M+H]1+), 369.19351.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutaminate

MS (ESI) m/z 549.1; MS (ESI) m/z 1097.2; RMS: calcd for C32H37ClN2O4+H+, 549.25146; found (ESI-FTMS, [M+H]1+), 549.25189.


Step D: 2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 493.1; HRMS: calcd for C28H29ClN2O4+H+, 493.18886; found (ESI-FTMS, [M+H]1+), 493.1895.


Example 8OO
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutaminate

MS (ESI) m/z 575.3; MS (ESI) m/z 1149.4; HRMS: calcd for C34H39FN2O5+H+, 575.29158; found (ESI-FTMS, [M+H]1+), 575.29261.


Step B: tert-butyl N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutaminate

MS (ESI) m/z 353.2; HRMS: calcd for C19H29FN2O3+H+, 353.22350; found (ESI-FTMS, [M+H]1+), 353.22526.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutaminate

MS (ESI) m/z 533.2; MS (ESI) m/z 1065.4; HRMS: calcd for C32H37FN2O4+H+, 533.28101; found (ESI-FTMS, [M+H]1+), 533.28063.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 477.2; MS (ESI) m/z 953.3; HRMS: calcd for C28H29FN2O4+H+, 477.21841; found (ESI-FTMS, [M+H]1+), 477.21783.


Example 8PP
N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine
Step A: tert-butyl N1-(1,1-dimethyl-2-phenylethyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 557.2; HRMS: calcd for C34H40N2O5+H+, 557.30100; found (ESI-FTMS, [M+H]1+), 557.2994.


Step B: tert-butyl N1-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 335.1; MS (ESI) m/z 669.3; HRMS: calcd for C19H30N2O3+H+, 335.23292; found (ESI-FTMS, [M+H]1+), 335.23259.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 515.2; MS (ESI) m/z 1029.3; HRMS: calcd for C32H38N2O4+H+, 515.29043; found (ESI-FTMS, [M+H]1+), 515.29018.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 459.1; MS (ESI) m/z 917.3; HRMS: calcd for C28H30N2O4+H+, 459.22783; found (ESI-FTMS, [M+H]1+), 459.22858.


Example 8QQ
N2-(biphenyl-4-ylcarbonyl)-N1-(3-isopropoxypropyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-isopropoxypropyl)-L-α-glutaminate

MS (ESI) m/z 525.3; MS (ESI) m/z 1049.5; HRMS: calcd for C30H40N2O6+H+, 525.29591; found (ESI-FTMS, [M+H]1+), 525.29528.


Step B: tert-butyl N1-(3-isopropoxypropyl)-L-α-glutaminate

MS (ESI) m/z 303.2; HRMS: calcd for C15H30N2O4+H+, 303.22783; found (ESI-FTMS, [M+H]1+), 303.22747.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(3-isopropoxypropyl)-L-α-glutaminate

MS (ESI) m/z 483.3; MS (ESI) m/z 965.5; HRMS: calcd for C28H38N2O5+H+, 483.28535; found (ESI-FTMS, [M+H]1+), 483.28364.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(3-isopropoxypropyl)-L-α-glutamine

MS (ESI) m/z 427.2; MS (ESI) m/z 853.4; MS (ESI) m/z 449.2; HRMS: calcd for C24H30N2O5+H+, 427.22275; found (ESI-FTMS, [M+H]1+), 427.22227.


Example 8RR
N2-(biphenyl-4-ylcarbonyl)-N1-(3-methylbutyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-methylbutyl)-L-α-glutaminate

MS (ESI) m/z 495.2; MS (ESI) m/z 989.5; HRMS: calcd for C29H38N2O5+H+, 495.28535; found (ESI-FTMS, [M+H]1+), 495.28729.


Step B: tert-butyl N1-(3-methylbutyl)-L-α-glutaminate

MS (ESI) m/z 273.2; HRMS: calcd for C14H28N2O3+H+, 273.21727; found (ESI-FTMS, [M+H]1+), 273.21718.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(3-methylbutyl)-L-α-glutaminate

MS (ESI) m/z 453.3; MS (ESI) m/z 905.5; MS (ESI) m/z 475.2; HRMS: calcd for C27H36N2O4+H+, 453.27478; found (ESI-FTMS, [M+H]1+), 453.27427.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(3-methylbutyl)-L-α-glutamine

MS (ESI) m/z 397.2; MS (ESI) m/z 793.3; HRMS: calcd for C23H28N2O4+H+, 397.21218; found (ESI-FTMS, [M+H]1+), 397.21129.


Example 8SS
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(4-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 547.2; MS (ESI) m/z 1093.3; HRMS: calcd for C32H35FN2O5+H+, 547.26028; found (ESI-FTMS, [M+H]1+), 547.26067.


Step B: tert-butyl N1-[2-(4-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 325.1; HRMS: calcd for C17H25FN2O3+H+, 325.19220; found (ESI-FTMS, [M+H]1+), 325.19212.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 505.2; MS (ESI) m/z 1009.3; HRMS: calcd for C30H33FN2O4+H+, 505.24971; found (ESI-FTMS, [M+H]1+), 505.25021.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 449.1; MS (ESI) m/z 897.2; HRMS: calcd for C26H25FN2O4+H+, 449.18711; found (ESI-FTMS, [M+H]1+), 449.18705.


Example 8TT
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-fluorophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(2-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 547.2; MS (ESI) m/z 1093.3; HRMS: calcd for C32H35FN2O5+H+, 547.26028; found (ESI-FTMS, [M+H]1+), 547.26138.


Step B: tert-butyl N1-[2-(2-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 325.1; MS (ESI) m/z 649.3; HRMS: calcd for C17H25FN2O3+H+, 325.19220; found (ESI-FTMS, [M+H]1+), 325.19217.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-fluorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 505.2; MS (ESI) m/z 1009.3; HRMS: calcd for C30H33FN2O4+H+, 505.24971; found (ESI-FTMS, [M+H]1+), 505.2497.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-fluorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 449.1; MS (ESI) m/z 897.2; HRMS: calcd for C26H25FN2O4+H+, 449.18711; found (ESI-FTMS, [M+H]1+), 449.18744.


Example 8UU
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(4-chlorophenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 563.1; MS (ESI) m/z 1125.2; HRMS: calcd for C32H35ClN2O5+Na+, 585.21267; found (ESI-FTMS, [M+Na]1+), 585.21454.


Step B: tert-butyl N1-[2-(4-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 341.1; MS (ESI) m/z 681.2; HRMS: calcd for C17H25ClN2O3+H+, 341.16265; found (ESI-FTMS, [M+H]1+), 341.16311.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 521.2; MS (ESI) m/z 1041.3; HRMS: calcd for C30H33ClN2O4+Na+, 543.20210; found (ESI-FTMS, [M+Na]1+), 543.20393.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-chlorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 463.1; MS (ESI) m/z 927.1; HRMS: calcd for C26H25ClN2O4+H+, 465.15756; found (ESI-FTMS, [M+H]1+), 465.15776.


Example 8VV
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-chlorophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(2-chlorophenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 563.1; MS (ESI) m/z 1125.2; HRMS: calcd for C32H35ClN2O5+H+, 563.23073; found (ESI-FTMS, [M+H]1+), 563.23042.


Step B: tert-butyl N1-[2-(2-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 341.2; MS (ESI) m/z 681.3; MS (ESI) m/z 703.3; HRMS: calcd for C17H25ClN2O3+H+, 341.16265; found (ESI-FTMS, [M+H]1+), 341.16301.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 521.2; MS (ESI) m/z 1041.4; HRMS: calcd for C30H33ClN2O4+H+, 521.22016; found (ESI-FTMS, [M+H]1+), 521.22075.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-chlorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 465; MS (ESI) m/z 487; HRMS: calcd for C26H25ClN2O4+H+, 465.15756; found (ESI-FTMS, [M+H]1+), 465.15834.


Example 8WW
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-thienyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(2-thienyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 535.1; MS (ESI) m/z 1069.2; HRMS: calcd for C30H34N2O5S+H+, 535.22612; found (ESI-FTMS, [M+H]1+), 535.22756.


Step B: tert-butyl N1-[2-(2-thienyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 313.1; MS (ESI) m/z 625.2; HRMS: calcd for C15H24N2O3S+H+, 313.15804; found (ESI-FTMS, [M+H]1+), 313.15788.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-thienyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 493.2; MS (ESI) m/z 985.4; HRMS: calcd for C28H32N2O4S+H+, 493.21555; found (ESI-FTMS, [M+H]1+), 493.21561.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2-thienyl)ethyl]-L-α-glutamine

MS (ESI) m/z 437.1; MS (ESI) m/z 873.3; HRMS: calcd for C24H24N2O4S+H+, 437.15295; found (ESI-FTMS, [M+H]1+), 437.15163.


Example 8XX
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-fluorobenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 533.2; HRMS: calcd for C31H33FN2O5+H+, 533.24463; found (ESI_FTMS, [M+H]1+), 533.2447.


Step B: tert-butyl N1-(3-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 311.2; HRMS: calcd for C16H23FN2O3+H+, 311.17655; found (ESI_FTMS, [M+H]1+), 311.1764.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 491.2; MS (ESI) m/z 981.4; HRMS: calcd for C29H31FN2O4+H+, 491.23406; found (ESI_FTMS, [M+H]1+), 491.2339.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-fluorobenzyl)-L-α-glutamine

MS (ESI) m/z 435.1; MS (ESI) m/z 869.2; HRMS: calcd for C25H23FN2O4+H+, 435.17146; found (ESI_FTMS, [M+H]1+), 435.1712.


Example 8YY
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-chlorophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(3-chlorophenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS m/z 03-305496LMS; HRMS: calcd for C32H35ClN2O5+H+, 563.23073; found (ESI_FTMS, [M+H]1+), 563.2309.


Step B: tert-butyl N1-[2-(3-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 341.1; HRMS: calcd for C17H25ClN2O3+H+, 341.16265; found (ESI_FTMS, [M+H]1+), 341.1611.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-chlorophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 521.1; MS (ESI) m/z 1041.1; HRMS: calcd for C30H33ClN2O4+H+, 521.22016; found (ESI-FTMS, [M+H]1+), 521.22136.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(3-chlorophenyl)ethyl]-L-α-glutamine

MS (ESI−) m/z 463.2; MS (ESI−) m/z 197; HRMS: calcd for C26H25ClN2O4+H+, 465.15756; found (ESI-FTMS, [M+H]1+), 465.15709.


Example 8ZZ
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-fluorobenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 533.1; MS (ESI) m/z 1065.1; HRMS: calcd for C31H33FN2O5+H+, 533.24463; found (ESI_FTMS, [M+H]1+), 533.24461.


Step B: tert-butyl N1-(2-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 311.1; MS (ESI) m/z 621.2; HRMS: calcd for C16H23FN2O3+H+, 311.17655; found (ESI_FTMS, [M+H]1+), 311.1762.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-fluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 491.1; MS (ESI) m/z 981.2; HRMS: calcd for C29H31FN2O4+H+, 491.23406; found (ESI-FTMS, [M+H]1+), 491.23526.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-fluorobenzyl)-L-α-glutamine

MS (ESI−) m/z 433.1; HRMS: calcd for C25H23FN2O4+H+, 435.17146; found (ESI-FTMS, [M+H]1+), 435.17161.


Example 8AAA
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-difluorobenzyl)-L-α-glutamine
Step A: tert-butyl N1-(3,4-difluorobenzyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 551.2; MS (ESI) m/z 1101.4; HRMS: calcd for C31H32F2N2O5+H+, 551.23521; found (ESI_FT, [M+H]1+), 551.23407.


Step B: tert-butyl N1-(3,4-difluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 329; MS (ESI) m/z 657.2; HRMS: calcd for C16H22F2N2O3+H+, 329.16712; found (ESI_FTMS, [M+H]1+), 329.1664.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-difluorobenzyl)-L-α-glutaminate

MS (ESI) m/z 509.1; MS (ESI) m/z 1017.1; HRMS: calcd for C29H30F2N2O4+H+, 509.22464; found (ESI-FTMS, [M+H]1+), 509.22485.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-difluorobenzyl)-L-α-glutamine

MS (ESI−) m/z 451.1; HRMS: calcd for C25H22F2N2O4+H+, 453.16204; found (ESI-FTMS, [M+H]1+), 453.1614.


Example 8BBB
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(sec-butyl)-L-α-glutamine
Step A: tert-butyl N1-(sec-butyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 481.2; HRMS: calcd for C28H36N2O5+H+, 481.26970; found (ESI-FTMS, [M+H]1+), 481.26966.


Step B: tert-butyl N1-(sec-butyl)-L-α-glutaminate

MS (ESI) m/z 259.1; HRMS: calcd for C13H26N2O3+H+, 259.20162; found (ESI-FTMS, [M+H]1+), 259.20164.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(sec-butyl)-L-α-glutaminate

MS (ESI) m/z 439.2; MS (ESI) m/z 877.4; HRMS: calcd for C26H34N2O4+H+, 439.25913; found (ESI-FTMS, [M+H]1+), 439.25972.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(sec-butyl)-L-α-glutamine

MS (ESI) m/z 383.1; MS (ESI) m/z 765.3; MS (ESI) m/z 441.2; HRMS: calcd for C22H26N2O4+H+, 383.19653; found (ESI-FTMS, [M+H]1+), 383.19745.


Example 8CCC
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(cyclopropylmethyl)-L-α-glutamine
Step A: tert-butyl N1-(cyclopropylmethyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 479.2; MS (ESI) m/z 957.3; HRMS: calcd for C28H34N2O5+H+, 479.25405; found (ESI-FTMS, [M+H]1+), 479.25563.


Step B: tert-butyl N1-(cyclopropylmethyl)-L-α-glutaminate

MS (ESI) m/z 257.1; HRMS: calcd for C13H24N2O3+H+, 257.18597; found (ESI-FTMS, [M+H]1+), 257.18608.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(cyclopropylmethyl)-L-α-glutaminate

MS (ESI) m/z 437.2; MS (ESI) m/z 873.3; HRMS: calcd for C26H32N2O4+H+, 437.24348; found (ESI-FTMS, [M+H]1+), 437.24446.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(cyclopropylmethyl)-L-α-glutamine

MS (ESI) m/z 381.1; MS (ESI) m/z 761.2; HRMS: calcd for C22H24N2O4+H+, 381.18088; found (ESI-FTMS, [M+H]1+), 381.18186.


Example 8DDD
N2-(biphenyl-4-ylcarbonyl)-N1-(2-morpholin-4-ylethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-morpholin-4-ylethyl)-L-α-glutaminate

MS (ESI) m/z 538.3; HRMS: calcd for C30H39N3O6+H+, 538.29116; found (ESI-FTMS, [M+H]1+), 538.29233.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(2-morpholin-4-ylethyl)-L-α-glutaminate

MS (ESI) m/z 496.2; HRMS: calcd for C28H37N3O5+H+, 496.28060; found (ESI-FTMS, [M+H]1+), 496.28079.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(2-morpholin-4-ylethyl)-L-α-glutamine

MS (ESI) m/z 440.2; HRMS: calcd for C24H29N3O5+H+, 440.21800; found (ESI-FTMS, [M+H]1+), 440.21827.


Example 8EEE
N2-(biphenyl-4-ylcarbonyl)-N1,N1-diethyl-L-α-glutamine
Step A: tert-butyl N1,N1-diethyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 481.3; MS (ESI) m/z 961.5; HRMS: calcd for C28H36N2O5+Na+, 503.25164; found (ESI-FTMS, [M+Na]1+), 503.25163.


Step B: tert-butyl N1,N1-diethyl-L-α-glutaminate

MS (ESI) m/z 259.2; HRMS: calcd for C13H26N2O3+H+, 259.20162; found (ESI-FTMS, [M+H]1+), 259.20149.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1,N1-diethyl-L-α-glutaminate

MS (ESI) m/z 439.2; MS (ESI) m/z 877.4; HRMS: calcd for C26H34N2O4+H+, 439.25913; found (ESI-FTMS, [M+H]1+), 439.26093.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1,N1-diethyl-L-α-glutamine

MS (ESI) m/z 383.2; MS (ESI) m/z 765.4; HRMS: calcd for C22H26N2O4+H+, 383.19653; found (ESI-FTMS, [M+H]1+), 383.19675.


Example 8FFF
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-phenylethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(2-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 529.2; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FTMS, [M+H]1+), 529.2693.


Step B: tert-butyl N1-(2-phenylethyl)-L-α-glutaminate

HRMS: calcd for C17H26N2O3+H+, 307.20162; found (ESI_FTMS, [M+H]1+), 307.2011.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 487.2; MS (ESI) m/z 973.3; HRMS: calcd for C30H34N2O4+H+, 487.25913; found (ESI_FTMS, [M+H]1+), 487.2588.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 431.2; MS (ESI) m/z 861.3; HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI_FTMS, [M+H]1+), 431.1963.


Example 8GGG
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethyl)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[3-(trifluoromethyl)benzyl]-L-α-glutaminate

MS (ESI) m/z 583.2; HRMS: calcd for C32H33F3N2O5+H+, 583.24143; found (ESI_FTMS, [M+H]1+), 583.2409.


Step B: tert-butyl N1-[3-(trifluoromethyl)benzyl]-L-α-glutaminate

HRMS: calcd for C17H23F3N2O3+H+, 361.17335; found (ESI_FTMS, [M+H]1+), 361.173.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethyl)benzyl]-L-α-glutaminate

MS (ESI) m/z 541.2; MS (ESI) m/z 1081.3; HRMS: calcd for C30H31F3N2O4+H+, 541.23087; found (ESI_FTMS, [M+H]1+), 541.2301.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[3-(trifluoromethyl)benzyl]-L-α-glutamine

MS (ESI) m/z 485.2; MS (ESI) m/z 969.3; HRMS: calcd for C26H23F3N2O4+H+, 485.16827; found (ESI_FTMS, [M+H]1+), 485.1682.


Example 8HHH
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(trifluoromethyl)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(trifluoromethyl)benzyl]-L-α-glutaminate

MS (ESI) m/z 583.2; HRMS: calcd for C32H33F3N2O5+H+, 583.24143; found (ESI_FT, [M+H]1+), 583.24162.


Step B: tert-butyl N1-[2-(trifluoromethyl)benzyl]-L-α-glutaminate

HRMS: calcd for C17H23F3N2O3+H+, 361.17335; found (ESI_FTMS, [M+H]1+), 361.1725.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(trifluoromethyl)benzyl]-L-α-glutaminate

MS (ESI) m/z 541.2; MS (ESI) m/z 1081.2; HRMS: calcd for C30H31F3N2O4+H+, 541.23087; found (ESI_FTMS, [M+H]1+), 541.23091.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[2-(trifluoromethyl)benzyl]-L-α-glutamine

MS (ESI) m/z 485; MS (ESI) m/z 969; HRMS: calcd for C26H23F3N2O4+H+, 485.16827; found (ESI_FTMS, [M+H]1+), 485.168.


Example 8III
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-methylbenzyl)-L-α-glutaminate

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.4; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FT, [M+H]1+), 529.26796.


Step B: tert-butyl N1-(4-methylbenzyl)-L-α-glutaminate

HRMS: calcd for C17H26N2O3+H+, 307.20162; found (ESI-FTMS, [M+H]1+), 307.20274.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methylbenzyl)-L-α-glutaminate

HRMS: calcd for C30H34N2O4+H+, 487.25913; found (ESI-FTMS, [M+H]1+), 487.25856.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methylbenzyl)-L-α-glutamine

HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI-FTMS, [M+H]1+), 431.19627.


Example 8JJJ
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-heptyl-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-heptyl-L-α-glutaminate

MS (ESI) m/z 523.3; MS (ESI) m/z 1045.5; HRMS: calcd for C31H42N2O5+H+, 523.31665; found (ESI-FTMS, [M+H]1+), 523.31783.


Step B: tert-butyl N1-heptyl-L-α-glutaminate

HRMS: calcd for C16H32N2O3+H+, 301.24857; found (ESI-FTMS, [M+H]1+), 301.2499.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-heptyl-L-α-glutaminate

HRMS: calcd for C29H40N2O4+H+, 481.30608; found (ESI-FTMS, [M+H]1+), 481.30706.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-heptyl-L-α-glutamine

HRMS: calcd for C25H32N2O4+H+, 425.24348; found (ESI-FTMS, [M+H]1+), 425.24417.


Example 8KKK
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-bromophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(4-bromophenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 607.1; HRMS: calcd for C32H35BrN2O5+H+, 607.18021; found (ESI-FTMS, [M+H]1+), 607.18182.


Step B: tert-butyl N1-[2-(4-bromophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 385; HRMS: calcd for C17H25BrN2O3+H+, 385.11213; found (ESI-FTMS, [M+H]1+), 385.11309.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-bromophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 565.1; MS (ESI) m/z 1129.1; HRMS: calcd for C30H33BrN2O4+H+, 565.16965; found (ESI-FTMS, [M+H]1+), 565.17.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-bromophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 507.2; HRMS: calcd for C26H25BrN2O4+H+, 509.10705; found (ESI-FTMS, [M+H]1+), 509.10906.


Example 8LLL
N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-dimethylbutyl)-L-α-glutamine
Step A: tert-butyl N1-(3,3-dimethylbutyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 509.3; MS (ESI) m/z 1017.5; HRMS: calcd for C30H40N2O5+H+, 509.30100; found (ESI-FTMS, [M+H]1+), 509.30165.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-dimethylbutyl)-L-α-glutaminate

MS (ESI) m/z 467.3; MS (ESI) m/z 933.5; MS (ESI) m/z 489.3; HRMS: calcd for C28H38N2O4+H+, 467.29043; found (ESI-FTMS, [M+H]1+), 467.29134.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-dimethylbutyl)-L-α-glutamine

HRMS: calcd for C24H30N2O4+H+, 411.22783; found (ESI-FTMS, [M+H]1+), 411.22929.


Example 8MMM
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-bromophenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N1-[2-(3-bromophenyl)ethyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 607.1; MS (ESI) m/z 1213.3; HRMS: calcd for C32H35BrN2O5+H+, 607.18021; found (ESI-FTMS, [M+H]1+), 607.18017.


Step B: tert-butyl N1-[2-(3-bromophenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 385.1; MS (ESI) m/z 769.2; HRMS: calcd for C17H25BrN2O3+H+, 385.11213; found (ESI-FTMS, [M+H]1+), 385.11175.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-bromophenyl)ethyl]-L-α-glutaminate

HRMS: calcd for C30H33BrN2O4+H+, 565.16965; found (ESI-FTMS, [M+H]1+), 565.16854.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-bromophenyl)ethyl]-L-α-glutamine

HRMS: calcd for C26H25BrN2O4+H+, 509.10705; found (ESI-FTMS, [M+H]1+), 509.10648.


Example 8NNN
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutaminate

MS (ESI) m/z 601.2; MS (ESI) m/z 1201.3; HRMS: calcd for C32H32F4N2O5+H+, 601.23201; found (ESI-FTMS, [M+H]1+), 601.23172.


Step B: tert-butyl N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutaminate

HRMS: calcd for C17H22F4N2O3+H+, 379.16393; found (ESI-FTMS, [M+H]1+), 379.16566.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutaminate

HRMS: calcd for C30H30F4N2O4+H+, 559.22145; found (ESI-FTMS, [M+H]1+), 559.22077.


Step D: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-[4-fluoro-3-(trifluoromethyl)benzyl]-L-α-glutamine

HRMS: calcd for C26H22F4N2O4+H+, 503.15885; found (ESI-FTMS, [M+H]1+), 503.1582.


Example 8OOO
N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-diphenylpropyl)-L-α-glutamine
Step A: tert-butyl N1-(3,3-diphenylpropyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 619.3; MS (ESI) m/z 1237.6; HRMS: calcd for C39H42N2O5+H+, 619.31665; found (ESI-FTMS, [M+H]1+), 619.31658.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-diphenylpropyl)-L-α-glutaminate

HRMS: calcd for C37H40N2O4+H+, 577.30608; found (ESI-FTMS, [M+H]1+), 577.30483.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(3,3-diphenylpropyl)-L-α-glutamine

HRMS: calcd for C33H32N2O4+H+, 521.24348; found (ESI-FTMS, [M+H]1+), 521.24166.


Example 8PPP
N2-(biphenyl-4-ylcarbonyl)-N1-isopropyl-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-isopropyl-L-α-glutaminate

MS (ESI) m/z 467.2; MS (ESI) m/z 933.5; HRMS: calcd for C27H34N2O5+Na+, 489.23599; found (ESI-FTMS, [M+Na]1+), 489.23577.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-isopropyl-L-α-glutaminate

MS (ESI) m/z 425.2; MS (ESI) m/z 447.2; MS (ESI) m/z 871.4; HRMS: calcd for C25H32N2O4+Na+, 447.22543; found (ESI-FTMS, [M+Na]1+), 447.22701.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-isopropyl-L-α-glutamine

MS (ESI) m/z 367.2; MS (ESI) m/z 735.3; MS (ESI) m/z 481.2; HRMS: calcd for C21H24N2O4+Na+, 391.16283; found (ESI-FTMS, [M+Na]1+), 391.16451.


Example 8QQQ
N2-(biphenyl-4-ylcarbonyl)-N1-(pyridin-4-ylmethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(pyridin-4-ylmethyl)-L-α-glutaminate

MS (ESI) m/z 516.3; HRMS: calcd for C30H33N3O5+H+, 516.24930; found (ESI_FTMS, [M+H]1+), 516.24875.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(pyridin-4-ylmethyl)-L-α-glutaminate

MS (ESI) m/z 474.2; HRMS: calcd for C28H31N3O4+H+, 474.23873; found (ESI-FTMS, [M+H]1+), 474.24265.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(pyridin-4-ylmethyl)-L-α-glutamine

HRMS: calcd for C24H23N3O4+H+, 418.17613; found (ESI-FTMS, [M+H]1+), 418.17812.


Example 8RRR
N2-(biphenyl-4-ylcarbonyl)-N1-pentyl-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-pentyl-L-α-glutaminate

MS (ESI) m/z 495.2; MS (ESI) m/z 989.5; HRMS: calcd for C29H38N2O5+Na+, 517.26729; found (ESI-FTMS, [M+Na]1+), 517.26785.


Step B: tert-butyl N1-pentyl-L-α-glutaminate

MS (ESI) m/z 273.2; HRMS: calcd for C14H28N2O3+H+, 273.21727; found (ESI-FTMS, [M+H]1+), 273.21761.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-pentyl-L-α-glutaminate

MS (ESI) m/z 453.2; MS (ESI) m/z 905.5; HRMS: calcd for C27H36N2O4+H+, 453.27478; found (ESI-FTMS, [M+H]1+), 453.27694.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-pentyl-L-α-glutamine

MS (ESI) m/z 397.2; MS (ESI) m/z 793.4; HRMS: calcd for C23H28N2O4+H+, 397.21218; found (ESI-FTMS, [M+H]1+), 397.2136.


Example 8SSS
N2-(biphenyl-4-ylcarbonyl)-N1-(tert-butyl)-L-α-glutamine
Step A: tert-butyl N1-(tert-butyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 481.2; MS (ESI) m/z 961.5; HRMS: calcd for C28H36N2O5+H+, 481.26970; found (ESI-FTMS, [M+H]1+), 481.27074.


Step B: tert-butyl N1-(tert-butyl)-L-α-glutaminate

MS (ESI) m/z 259.1; MS (ESI) m/z 517.3; HRMS: calcd for C13H26N2O3+H+, 259.20162; found (ESI-FTMS, [M+H]1+), 259.20174.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(tert-butyl)-L-α-glutaminate

MS (ESI) m/z 439.2; MS (ESI) m/z 877.4; HRMS: calcd for C26H34N2O4+H+, 439.25913; found (ESI-FTMS, [M+H]1+), 439.25922.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(tert-butyl)-L-α-glutamine

MS (ESI) m/z 383.2; MS (ESI) m/z 765.3; HRMS: calcd for C22H26N2O4+H+, 383.19653; found (ESI-FTMS, [M+H]1+), 383.19812.


Example 8TTT
N2-(biphenyl-4-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(1-methyl-1-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 543.2; MS (ESI) m/z 1085.4; HRMS: calcd for C33H38N2O5+H+, 543.28535; found (ESI-FTMS, [M+H]1+), 543.28707.


Step B: tert-butyl N1-(1-methyl-1-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 321.1; MS (ESI) m/z 641.3; HRMS: calcd for C18H28N2O3+H+, 321.21727; found (ESI-FTMS, [M+H]1+), 321.21716.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutaminate

MS (ESI) m/z 501.2; MS (ESI) m/z 1001.4; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI-FTMS, [M+H]1+), 501.27462.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutamine

HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI-FTMS, [M+H]1+), 445.21311.


Example 8UUU
N2-(biphenyl-4-ylcarbonyl)-N1-(2,2-dimethylpropyl)-L-α-glutamine
Step A: tert-butyl N1-(2,2-dimethylpropyl)-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 495.2; MS (ESI) m/z 989.4; HRMS: calcd for C29H38N2O5+H+, 495.28535; found (ESI-FTMS, [M+H]1+), 495.2859.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(2,2-dimethylpropyl)-L-α-glutaminate

MS (ESI) m/z 453.2; MS (ESI) m/z 905.4; HRMS: calcd for C27H36N2O4+H+, 453.27478; found (ESI-FTMS, [M+H]1+), 453.2764.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(2,2-dimethylpropyl)-L-α-glutamine

MS (ESI) m/z 397.1; MS (ESI) m/z 793.3; HRMS: calcd for C23H28N2O4+H+, 397.21218; found (ESI-FTMS, [M+H]1+), 397.21242.


Example 8VVV
N2-(biphenyl-4-ylcarbonyl)-N1-hexyl-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-hexyl-L-α-glutaminate

MS (ESI) m/z 509.3; MS (ESI) m/z 1017.5; HRMS: calcd for C30H40N2O5+H+, 509.30100; found (ESI-FTMS, [M+H]1+), 509.30265.


Step B: tert-butyl N1-hexyl-L-α-glutaminate

HRMS: calcd for C15H30N2O3+H+, 287.23292; found (ESI-FTMS, [M+H]1+), 287.23282.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-hexyl-L-α-glutaminate

MS (ESI) m/z 467.2; MS (ESI) m/z 933.4; HRMS: calcd for C28H38N2O4+H+, 467.29043; found (ESI-FTMS, [M+H]1+), 467.29169.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-hexyl-L-α-glutamine

MS (ESI) m/z 411.1; MS (ESI) m/z 821.3; HRMS: calcd for C24H30N2O4+H+, 411.22783; found (ESI-FTMS, [M+H]1+), 411.22833.


Example 8WWW
N2-(biphenyl-4-ylcarbonyl)-N1-(5-hydroxypentyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(5-hydroxypentyl)-L-α-glutaminate

MS (ESI) m/z 511.2; MS (ESI) m/z 1021.4; HRMS: calcd for C29H38N2O6+H+, 511.28026; found (ESI-FTMS, [M+H]1+), 511.28078.


Step B: tert-butyl N1-(5-hydroxypentyl)-L-α-glutaminate

HRMS: calcd for C14H28N2O4+H+, 289.21218; found (ESI-FTMS, [M+H]1+), 289.21245.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(5-hydroxypentyl)-L-α-glutaminate

HRMS: calcd for C27H36N2O5+H+, 469.26970; found (ESI-FTMS, [M+H]1+), 469.27133.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(5-hydroxypentyl)-L-α-glutamine

HRMS: calcd for C23H28N2O5+H+, 413.20710; found (ESI-FTMS, [M+H]1+), 413.20657.


Example 8XXX
N2-(biphenyl-4-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(6-hydroxyhexyl)-L-α-glutaminate

MS (ESI) m/z 525.2; MS (ESI) m/z 1049.4; HRMS: calcd for C30H40N2O6+Na+, 547.27786; found (ESI-FTMS, [M+Na]1+), 547.27869.


Step B: tert-butyl N1-(6-hydroxyhexyl)-L-α-glutaminate

HRMS: calcd for C15H30N2O4+H+, 303.22783; found (ESI-FTMS, [M+H]1+), 303.22841.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutaminate

HRMS: calcd for C28H38N2O5+H+, 483.28535; found (ESI-FTMS, [M+H]1+), 483.28725.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine

HRMS: calcd for C24H30N2O5+H+, 427.22275; found (ESI-FTMS, [M+H]1+), 427.22304.


Example 8YYY
N2-(biphenyl-4-ylcarbonyl)-N1-propyl-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-propyl-L-α-glutaminate

MS (ESI) m/z 467.1; MS (ESI) m/z 933.1; MS (ESI) m/z 955.1; HRMS: calcd for C27H34N2O5+H+, 467.25405; found (ESI-FTMS, [M+H]1+), 467.25527.


Step B: tert-butyl N1-propyl-L-α-glutaminate

MS (ESI) m/z 245.1; MS (ESI) m/z 489.2; HRMS: calcd for C12H24N2O3+H+, 245.18597; found (ESI-FTMS, [M+H]1+), 245.1861.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-propyl-L-α-glutaminate

HRMS: calcd for C25H32N2O4+H+, 425.24348; found (ESI-FTMS, [M+H]1+), 425.24455.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-propyl-L-α-glutamine

HRMS: calcd for C21H24N2O4+H+, 369.18088; found (ESI-FTMS, [M+H]1+), 369.18091.


Example 8ZZZ
N2-(biphenyl-4-ylcarbonyl)-N1-[4-(dimethylamino)benzyl]-L-α-glutamine
Step A: tert-butyl N1-[4-(dimethylamino)benzyl]-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 558.2; HRMS: calcd for C33H39N3O5+H+, 558.29625; found (ESI-FTMS, [M+H]1+), 558.29668.


Step B: tert-butyl N1-[4-(dimethylamino)benzyl]-L-α-glutaminate

MS (ESI) m/z 336.2; MS (ESI) m/z 671.3; HRMS: calcd for C18H29N3O3+H+, 336.22817; found (ESI-FTMS, [M+H]1+), 336.22836.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[4-(dimethylamino)benzyl]-L-α-glutaminate

MS (ESI) m/z 516.2; HRMS: calcd for C31H37N3O4+H+, 516.28568; found (ESI-FTMS, [M+H]1+), 516.28631.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[4-(dimethylamino)benzyl]-L-α-glutamine

MS (ESI) m/z 460.1; HRMS: calcd for C27H29N3O4+H+, 460.22308; found (ESI-FTMS, [M+H]1+), 460.22347.


Example 8AAAA
N1-(4-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(4-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 560.3; MS (ESI) m/z 1119.5; HRMS: calcd for C31H33N3O7+H+, 560.23913; found (ESI_FTMS, [M+H]1+), 560.23944.


Step B: tert-butyl N1-(4-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 338.2; HRMS: calcd for C16H23N3O5+H+, 338.17105; found (ESI-FTMS, [M+H]1+), 338.17116.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 518.2; MS (ESI) m/z 1035.5; HRMS: calcd for C29H31N3O6+H+, 518.22856; found (ESI-FTMS, [M+H]1+), 518.22861.


Step C′: Reductive alkylation of tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-nitrobenzyl)-L-α-glutaminate with 30% formalin in water and 10% Pd/C gave tert-butyl N1-(4-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutaminate


MS (ESI) m/z 488.2; MS (ESI) m/z 975.4; HRMS: calcd for C29H33N3O4+H+, 488.25438; found (ESI_FTMS, [M+H]1+), 488.2542.


Step D: N1-(4-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 432.2; HRMS: calcd for C25H25N3O4+H+, 432.19178; found (ESI_FTMS, [M+H]1+), 432.1915.


Example 8BBBB
N2-(biphenyl-4-ylcarbonyl)-N1-[3-(dimethylamino)benzyl]-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 560.3; MS (ESI) m/z 1119.4; HRMS: calcd for C31H33N3O7+H+, 560.23913; found (ESI_FTMS, [M+H]1+), 560.23892.


Step B: tert-butyl N1-(3-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 338.1; MS (ESI) m/z 675.3; HRMS: calcd for C16H23N3O5+H+, 338.17105; found (ESI-FTMS, [M+H]1+), 338.17102.


Step C: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-nitrobenzyl)-L-α-glutaminate

MS (ESI) m/z 518.2; MS (ESI) m/z 1035.4; HRMS: calcd for C29H31N3O6+H+, 518.22856; found (ESI-FTMS, [M+H]1+), 518.22868.


Step C′: Reductive alkylation of tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-nitrobenzyl)-L-α-glutaminate 30% formalin and 10% Pd/C gave tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[3-(dimethylamino)benzyl]-L-α-glutaminate


MS (ESI) m/z 516.2; HRMS: calcd for C31H37N3O4+H+, 516.28568; found (ESI-FTMS, [M+H]1+), 516.28548.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[3-(dimethylamino)benzyl]-L-α-glutamine

MS (ESI) m/z 460.3; HRMS: calcd for C27H29N3O4+H+, 460.22308; found (ESI-FTMS, [M+H]1+), 460.22304.


Example 8CCCC
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-nitrobenzyl)-L-α-glutamine

MS (ESI) m/z 462.2; MS (ESI) m/z 923.2; HRMS: calcd for C25H23N3O6+H+, 462.16596; found (ESI_FTMS, [M+H]1+), 462.16599.


Example 8DDDD
N1-(3-aminobenzyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

HRMS: calcd for C25H25N3O4+H+, 432.19178; found (ESI_FTMS, [M+H]1+), 432.1917.


Example 8EEEE
N1-benzyl-N2-(1,1-biphenyl-4-ylcarbonyl)-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-α-glutaminate

MS (ESI) m/z 458 (M-tBut).


Step B: tert-butyl N1-benzyl-L-α-glutaminate

MS (ESI) m/z 293.


Step C: tert-butyl N1-benzyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutaminate

MS (ESI) m/z 473.


Step D: N1-benzyl-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 417.


Example 8FFFF
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutaminate

Described as above according to the procedure of Example 7, except Fmoc-D-Glu-(OtBu)-OH was used as starting material for the first step. HRMS: calcd for C34H39FN2O5+H+, 575.29158; found (ESI-FTMS, [M+H]1+), 575.2927.


Step B: tert-butyl N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutaminate

HRMS: calcd for C19H29FN2O3+H+, 353.22350; found (ESI-FTMS, [M+H]1+), 353.22483.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutaminate

HRMS: calcd for C32H37FN2O4+H+, 533.28101; found (ESI-FTMS, [M+H]1+), 533.28347.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutamine

MS (ESI) m/z 477.1; MS (ESI) m/z 499; MS (ESI) m/z 953.1; HRMS: calcd for C28H29FN2O4+H+, 477.21841; found (ESI-FTMS, [M+H]1+), 477.21815.


Example 8GGGG
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutaminate

Described as above according to the procedure of Example 7, except Fmoc-DL-Glu-(OtBu)-OH (racemic mixture) was used as starting material for the first step. HRMS: calcd for C34H39FN2O5+H+, 575.29158; found (ESI-FTMS, [M+H]1+), 575.29217.


Step B: tert-butyl N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutaminate

HRMS: calcd for C19H29FN2O3+H+, 353.22350; found (ESI-FTMS, [M+H]1+), 353.22429.


Step C: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutaminate

HRMS: calcd for C32H37FN2O4+H+, 533.28101; found (ESI-FTMS, [M+H]1+), 533.28268.


Step D: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-α-glutamine

MS (ESI) m/z 477.1; MS (ESI) m/z 953.1; HRMS: calcd for C28H29FN2O4+H+, 477.21841; found (ESI-FTMS, [M+H]1+), 477.21805.


Example 8HHHH
4-Benzylcarbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino-butyric acid tert-butyric acid
Step C: 4-Benzylcarbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino-butyric acid tert-butyl ester

MS (ESI) m/z 488.


Step D: 4-Benzylcarbamoyl-4-[4-(pyrimidin-2-ylamino)-benzoylamino-butyric acid tert-butyric acid

MS (ESI) m/z 432.


Example 8IIII
N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine
Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-phenylpropyl)-L-α-glutaminate

1,9(Fmoc)L-glutamic acid-tert-butylester monohydrate (1.33 g, 3.00 mmol) was dissolved in DMF (8 mL) under nitrogen. BOP (1.33 g, 3.00 mmol) was added as a solid, followed by 3-phenyl-1-propylamine (427 mL, 3 mmol). Finally DIEA (627 mL, 3.6 mmol) was added dropwise. The reaction was monitored by TLC, and stirred for 24 hrs. The reaction mixture was then added to stirring H2O (400 mL). After stirring for 1 hour, clumps of white solid were formed. The clumps were broken up with a spatula and stirred for an additional 30 minutes. The solid was filtered and washed with H2O. The solid was then dissolved in CH2Cl2 and dried with Na2SO4. The solution was filtered and concentrated, leaving a white solid. The solid was dissolved in minimal acetone and hexanes were added. The solvents were then removed and the white solid was dried under vacuum. Yield 1.58 g of tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-phenylpropyl)-L-α-glutaminate at 97% yield.


MS (ESI) m/z 543.3; HRMS: calcd for C33H38N2O5+H+, 543.2853; found (ESI-FTMS, [M+H]1+), 543.2847.


Step B: tert-butyl N1-(3-phenylpropyl)-L-α-glutaminate

Tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3-phenylpropyl)-L-α-glutaminate (1.5 g, 2.76 mmol) was partially dissolved in CH3CN (15 mL). DEA (2.9 mL, 27.6 mmol) was added, which was fully dissolved within 10 minutes. The reaction was monitored by TLC and was complete at 2 hrs. The solvent was removed and the remaining material dried under vacuum. The material was purified on a short silica column using 5% Acetone/CH2Cl2 to elute the Fmoc by-product and then 50% Acetone/CH2Cl2 to elute the product. The clear oil present at the end was dried shortly under vacuum, and then taken to the next step immediately. It was never fully solvent free so the yield of tert-butyl N1-(3-phenylpropyl)-L-α-glutaminate was 1.65 g, and was assumed to be quantitative. MS (ESI) m/z 321.2; MS (ESI) m/z 641.4; HRMS: calcd for C18H28N2O3+H+, 321.2173; found (ESI-FTMS, [M+H]1+), 321.2167.


Step C: tert-butyl N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutaminate

Tert-butyl N1-(3-phenylpropyl)-L-α-glutaminate (350 mg, 1.09 mmol) was dissolved in DMF (5 mL), in a 20 mL vial under nitrogen. 2-Fluorenecarboxylic acid (229 mg, 1.09 mmol) and BOP (530 mg, 1.20 mmol) were added as solids and dissolved. Finally DIEA (228 mL, 1.31 mmol) was added, and the reaction stirred overnight. Monitored by TLC, the reaction was complete after stirring overnight. The reaction mixture was added to H2O (100 mL), precipitating a pale yellow solid. The solid was filtered and dried in a dessicator overnight. Once dry, the solid was taken up in a small amount of acetone, and hexanes were added to precipitate a pale yellow solid. The solid was filtered, washed with hexanes and dried in a dessicator. Yield 293 mg of tert-butyl N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutaminate at 52% yield. MS (ESI) m/z 513.3; MS (ESI) m/z 1025.6; MS (ESI) m/z 535.3; HRMS: calcd for C32H36N2O4+H+, 513.27478; found (ESI-FTMS, [M+H]1+), 513.2766.


Step D: N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine

Tert-butyl N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutaminate (273 mg, 0.533 mmol) was dissolved in CH2Cl2 (1.5 mL). TFA (1.23 mL, 16.0 mmol) was diluted in CH2Cl2 (2 mL) and was then slowly added to the first solution. The reaction was monitored by TLC and complete at 2 hrs. The solvent was removed with a nitrogen blower and the remaining yellow oil dried under vacuum. The yellow oil was then dissolved in minimal acetone and hexanes was added precipitating a white solid. The solid was filtered, washed with hexanes and then dried under vacuum. The reaction yielded 194 mg of N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine at an 80% yield. MS (ESI) m/z 457.3; MS (ESI) m/z 913.5.


Example 8JJJJ
N2-(9H-fluoren-1-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine
Step C: tert-butyl N2-(9H-fluoren-1-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutaminate

MS (ESI) m/z 513.3; MS (ESI) m/z 1025.6; HRMS: calcd for C32H36N2O4+H+, 513.27478; found (ESI-FTMS, [M+H]1+), 513.27653.


Step D: N2-(9H-fluoren-1-ylcarbonyl)-N1-(3-phenylpropyl)-L-α-glutamine

MS (ESI) m/z 457.3; MS (ESI) m/z 913.5; MS (ESI) m/z 479.3.


Example 8KKKK
N2-(9H-fluoren-2-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine






Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 605.2; MS (ESI) m/z 1209.4; HRMS: calcd for C34H40N2O8+H+, 605.28574; found (ESI_FT, [M+H]1+), 605.28453.


Step B: tert-butyl N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 383.1; MS (ESI) m/z 765.3; HRMS: calcd for C19H30N2O6+H+, 383.21766; found (ESI_FT, [M+H]1+), 383.21755.


Step C: tert-butyl N2-(9H-fluoren-2-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 575.4; MS (ESI) m/z 1149.7; HRMS: calcd for C33H38N2O7+H+, 575.27518; found (ESI-FTMS, [M+H]1+), 575.27604.


Step D: N2-(9H-fluoren-2-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 519.3; MS (ESI) m/z 1037.6; HRMS: calcd for C29H30N2O7+H+, 519.21258; found (ESI-FTMS, [M+H]1+), 519.2133.


Example 8LLLL
N2-(9H-fluoren-1-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine
Step C: tert-butyl N2-(9H-fluoren-1-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 575.4; MS (ESI) m/z 1149.7; HRMS: calcd for C33H38N2O7+H+, 575.27518; found (ESI-FTMS, [M+H]1+), 575.2761.


Step D: N2-(9H-fluoren-1-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 519.3; MS (ESI) m/z 1037.6; MS (ESI) m/z 541.3; HRMS: calcd for C29H30N2O7+H+, 519.21258; found (ESI-FTMS, [M+H]1+), 519.21329.


Example 8MMMM
N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine
Step C: tert-butyl N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 589.4; MS (ESI) m/z 1177.7; MS (ESI) m/z 606.4; HRMS: calcd for C33H36N2O8+H+, 589.25444; found (ESI-FTMS, [M+H]1+), 589.25507.


Step D: N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 531.3; MS (ESI) m/z 1063.5; HRMS: calcd for C29H28N2O8+H+, 533.19184; found (ESI-FTMS, [M+H]1+), 533.19236.


Example 8NNNN
N2-(4-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine
Step C: tert-butyl N2-(4-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 579.3; MS (ESI) m/z 1157.7; HRMS: calcd for C32H38N2O8+H+, 579.27009; found (ESI-FTMS, [M+H]1+), 579.2719.


Step D: N2-(4-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 523.3; MS (ESI) m/z 1045.5.


Example 8OOOO
N2-(9H-fluoren-2-ylcarbonyl)-N1-hexyl-L-α-glutamine






Step A: tert-butyl N2-[(9H-fluoren-9-ylmethoxy)carbonyl]-N1-hexyl-L-α-glutaminate

MS (ESI) m/z 509.3; MS (ESI) m/z 1017.5; HRMS: calcd for C30H40N2O5+H+, 509.30100; found (ESI-FTMS, [M+H]1+), 509.30265.


Step B: tert-butyl N1-hexyl-L-α-glutaminate

HRMS: calcd for C15H30N2O3+H+, 287.23292; found (ESI-FTMS, [M+H]1+), 287.23282.


Step C: tert-butyl N2-(9H-fluoren-2-ylcarbonyl)-N1-hexyl-L-α-glutaminate

MS (ESI) m/z 479.1; MS (ESI) m/z 979.2; MS (ESI) m/z 501.1; HRMS: calcd for C29H38N2O4+H+, 479.29043; found (ESI-FTMS, [M+H]1+), 479.29145.


Step D: N2-(9H-fluoren-2-ylcarbonyl)-N1-hexyl-L-α-glutamine

MS (ESI) m/z 423.3; MS (ESI) m/z 845.6; MS (ESI) m/z 445.3.


Example 8PPPP
N2-(9H-fluoren-1-ylcarbonyl)-N1-hexyl-L-α-glutamine
Step C: tert-butyl N2-(9H-fluoren-1-ylcarbonyl)-N1-hexyl-L-α-glutaminate

MS (ESI) m/z 479.1; MS (ESI) m/z 957.3; MS (ESI) m/z 979.2; HRMS: calcd for C29H38N2O4+H+, 479.29043; found (ESI-FTMS, [M+H]1+), 479.29145.


Step D: N2-(9H-fluoren-1-ylcarbonyl)-N1-hexyl-L-α-glutamine

MS (ESI) m/z 423.3; MS (ESI) m/z 845.5; MS (ESI) m/z 445.3.


Example 8QQQQ
N1-hexyl-N2-(4-phenoxybenzoyl)-L-α-glutamine
Step C: tert-butyl N1-hexyl-N2-(4-phenoxybenzoyl)-L-α-glutaminate

MS (ESI) m/z 483.1; MS (ESI) m/z 965.3; MS (ESI) m/z 987.3; HRMS: calcd for C28H38N2O5+H+, 483.28535; found (ESI-FTMS, [M+H]1+), 483.28715.


Step D: N1-hexyl-N2-(4-phenoxybenzoyl)-L-α-glutamine

MS (ESI) m/z 427.3; MS (ESI) m/z 853.5; MS (ESI) m/z 449.3.


Example 8RRRR
N1-hexyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine
Step C: tert-butyl N1-hexyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 493.1; MS (ESI) m/z 985.3; HRMS: calcd for C29H36N2O5+H+, 493.26970; found (ESI-FTMS, [M+H]1+), 493.27119.


Step D: N1-hexyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 437.3; MS (ESI) m/z 873.5; MS (ESI) m/z 1309.8.


Example 8SSSS
N2-(4-benzoylbenzoyl)-N1-benzyl-L-α-glutamine
Step C: tert-butyl N2-(4-benzoylbenzoyl)-N1-benzyl-L-α-glutaminate

MS (ESI) m/z 501.2; MS (ESI) m/z 523.2; HRMS: calcd for C30H32N2O5+H+, 501.23840; found (ESI+, [M+H]1+), 501.23859.


Step: D N2-(4-benzoylbenzoyl)-N1-benzyl-L-α-glutamine

MS (ESI) m/z 445.2; HRMS: calcd for C26H24N2O5+H+, 445.17580; found (ESI+, [M+H]1+), 445.17619.


Example 8TTTT
N1-(6-hydroxyhexyl)-N2-(4-phenoxybenzoyl)-L-α-glutamine

MS (ESI) m/z 443.2; MS (ESI) m/z 885.5; HRMS: calcd for C24H30N2O6+H+, 443.21766; found (ESI-FTMS, [M+H]1+), 443.21905.


Example 8UUUU
N2-(9H-fluoren-9-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine

MS (ESI) m/z 437.3; MS (ESI) m/z 551.3; HRMS: calcd for C25H30N2O5+H+, 439.22275; found (ESI-FTMS, [M+H]1+), 439.22374.


Example 8VVVV
N2-(9H-fluoren-9-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 519.1; MS (ESI) m/z 1037.2; MS (ESI) m/z 536.1; HRMS: calcd for C29H30N2O7+H+, 519.21258; found (ESI-FTMS, [M+H]1+), 519.213.


Example 8WWWW
N2-(3-phenoxybenzoyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 523.1; MS (ESI) m/z 1045.2; MS (ESI) m/z 545.1; HRMS: calcd for C28H30N2O8+H+, 523.20749; found (ESI-FTMS, [M+H]1+), 523.20712.


Example 8XXXX
N2-(9H-fluoren-4-ylcarbonyl)-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 519.1; MS (ESI) m/z 1037.2; HRMS: calcd for C29H30N2O7+H+, 519.21258; found (ESI-FTMS, [M+H]1+), 519.21308.


Example 8YYYY
N1-1-adamantyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 485.3; MS (ESI) m/z 971.6; MS (ESI) m/z 521.3; HRMS: calcd for C29H30N2O5+H+, 487.22275; found (ESI-FTMS, [M+H]1+), 487.22272.


Example 8ZZZZ
N1-1-adamantyl-N2-(4-phenoxybenzoyl)-L-α-glutamine

MS (ESI) m/z 477.1; MS (ESI) m/z 953.3; MS (ESI) m/z 499.1; HRMS: calcd for C28H32N2O5+H+, 477.23840; found (ESI-FTMS, [M+H]1+), 477.23857.


Example 8AAAAA
N1-1-adamantyl-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 473.3; MS (ESI) m/z 945.5; HRMS: calcd for C29H32N2O4+H+, 473.24348; found (ESI-FTMS, [M+H]1+), 473.24374.


Example 8BBBBB
N1-1-adamantyl-N2-(9H-fluoren-9-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 471.3; MS (ESI) m/z 585.3; MS (ESI) m/z 943.6; HRMS: calcd for C29H32N2O4+H+, 473.24348; found (ESI-FTMS, [M+H]1+), 473.2437.


Example 8CCCCC
N2-(9H-fluoren-2-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine

HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.1974.


Example 8DDDDD
N1-(3-methylbenzyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

HRMS: calcd for C27H24N2O5+H+, 457.17580; found (ESI-FTMS, [M+H]1+), 457.17616.


Example 8EEEEE
N2-(9H-fluoren-9-ylcarbonyl)-N1-(3-methylbenzyl)-L-α-glutamine

HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19709.


Example 8FFFFF
N2-(9H-fluoren-2-ylcarbonyl)-N1-propyl-L-α-glutamine

HRMS: calcd for C22H24N2O4+H+, 381.18088; found (ESI-FTMS, [M+H]1+), 381.18117.


Example 8GGGGG
4-[(9H-fluoren-9-carbonyl)-amino]-4-propylcarbamoyl-butyric acid

MS (ESI) m/z 395.1; MS (ESI) m/z 789.2; MS (ESI) m/z 811.2; HRMS: calcd for C22H24N2O4+H+, 381.18088; found (ESI-FTMS, [M+H]1+), 395.16097.


Example 8HHHHH
4-[(9-Oxo-9H-fluorene-2-carbonyl)-amino]-4-propylcarbamoyl-butyric acid

MS (ESI) m/z 381.1; MS (ESI) m/z 403.1; MS (ESI) m/z 761.2; HRMS: calcd for C22H22N2O5+H+, 395.16015; found (ESI-FTMS, [M+H]1+), 381.18165.


Example 8IIIII
N1-benzyl-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 441.2; MS (ESI) m/z 883.3; HRMS: calcd for C26H22N2O5+H+, 443.16015; found (ESI-FTMS, [M+H]1+), 443.16063.


Example 8JJJJJ
N1-benzyl-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 427.2; MS (ESI) m/z 855.3; HRMS: calcd for C26H24N2O4+H+, 429.18088; found (ESI-FTMS, [M+H]1+), 429.18148.


Example 8KKKKK
N1-benzyl-N2-(9H-fluoren-9-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 429; MS (ESI) m/z 857; MS (ESI) m/z 451; HRMS: calcd for C26H24N2O4+H+, 429.18088; found (ESI-FTMS, [M+H]1+), 429.1815.


Example 8LLLLL
N1-benzyl-N2-(3-phenoxybenzoyl)-L-α-glutamine

MS (ESI) m/z 433; MS (ESI) m/z 455; MS (ESI) m/z 865; HRMS: calcd for C25H24N2O5+H+, 433.17580; found (ESI-FTMS, [M+H]1+), 433.17696.


Example 8MMMMM
N1-(1-methyl-1-phenylethyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 471.2; MS (ESI) m/z 941.5; MS (ESI) m/z 493.2; HRMS: calcd for C28H26N2O5+H+, 471.19145; found (ESI-FTMS, [M+H]1+), 471.19271.


Example 8NNNNN
N2-(9H-fluoren-9-ylcarbonyl)-N1-(1-methyl-1-phenylethyl)-L-α-glutamine

MS (ESI) m/z 455.1; MS (ESI) m/z 911.3; HRMS: calcd for C28H28N2O4+H+, 457.21218; found (ESI-FTMS, [M+H]1+), 457.214.


Example 8OOOOO
N1-(1-methyl-1-phenylethyl)-N2-(3-phenoxybenzoyl)-L-α-glutamine

MS (ESI) m/z 461.2; MS (ESI) m/z 921.5; MS (ESI) m/z 483.2; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI-FTMS, [M+H]1+), 461.20882.


Example 8PPPPP
N2-(biphenyl-4-ylcarbonyl)-N1-[(1S)-1-phenylethyl]-L-α-glutamine

MS (ESI) m/z 431.2; MS (ESI) m/z 861.4; MS (ESI) m/z 453.2; HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI-FTMS, [M+H]1+), 431.19802.


Example 8QQQQQ
N2-(9H-fluoren-2-ylcarbonyl)-N1-[(1S)-1-phenylethyl]-L-α-glutamine

MS (ESI) m/z 443.2; MS (ESI) m/z 885.4; MS (ESI) m/z 465.2; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19772.


Example 8RRRRR
N2-(biphenyl-4-ylcarbonyl)-N1-[(1S)-1-(4-fluorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 449.2; MS (ESI) m/z 897.4; HRMS: calcd for C26H25FN2O4+H+, 449.18711; found (ESI-FTMS, [M+H]1+), 449.18839.


Example 8SSSSS
tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(1-Phenylethyl)-L-α-glutaminate

MS (ESI) m/z 505.3.


Example 8TTTTT
N2-(biphenyl-4-ylcarbonyl)-N1-[(1R)-1-(4-fluorophenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 449.2; MS (ESI) m/z 897.5; MS (ESI) m/z 471.2; HRMS: calcd for C26H25FN2O4+H+, 449.18711; found (ESI-FTMS, [M+H]1+), 449.18679.


Example 8UUUUU
N2-(biphenyl-4-ylcarbonyl)-N1-[(1R)-1-phenylethyl]-L-α-glutamine

MS (ESI) m/z 431.2; MS (ESI) m/z 861.5; MS (ESI) m/z 453.2; HRMS: calcd for C26H26N2O4+H+, 431.19653; found (ESI-FTMS, [M+H]1+), 431.19828.


Example 8VVVVV
N2-(9H-fluoren-2-ylcarbonyl)-N1-[(1R)-1-phenylethyl]-L-α-glutamine

MS (ESI) m/z 443.2; MS (ESI) m/z 885.5; MS (ESI) m/z 465.2; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19676.


Example 8WWWWW
N2-(9H-fluoren-1-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine

MS (ESI) m/z 437.3; MS (ESI) m/z 875.5; HRMS: calcd for C25H30N2O5+H+, 439.22275; found (ESI-FTMS, [M+H]1+), 439.22313.


Example 8XXXXX
N1-(6-hydroxyhexyl)-N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 453.2; HRMS: calcd for C25H28N2O6+H+, 453.20201; found (ESI-FTMS, [M+H]1+), 453.2027.


Example 8YYYYY
N2-(biphenyl-4-ylcarbonyl)-N1-9H-fluoren-9-yl-L-α-glutamine

MS (ESI) m/z 489.1; MS (ESI) m/z 979.2; HRMS: calcd for C31H26N2O4+H+, 491.19653; found (ESI-FTMS, [M+H]1+), 491.19778.


Example 8ZZZZZ
N2-(9H-fluoren-2-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 489.2; MS (ESI) m/z 977.4; HRMS: calcd for C29H29FN2O4+H+, 489.21841; found (ESI-FTMS, [M+H]1+), 489.21827.


Example 8AAAAAA
N-(1-adamantylmethyl)-N2-(biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 475.2; MS (ESI) m/z 949.5; HRMS: calcd for C29H34N2O4+H+, 475.25913; found (ESI-FTMS, [M+H]1+), 475.25916.


Example 8BBBBBB
N-[(1S)-1-benzyl-2-hydroxyethyl]-N2-(9H-fluoren-2-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 473.2; MS (ESI) m/z 945.4; HRMS: calcd for C28H28N2O5+H+, 473.20710; found (ESI-FTMS, [M+H]1+), 473.2073.


Example 8CCCCCC
N2-(biphenyl-4-ylcarbonyl)-N-methyl-N-(2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 445.4; MS (ESI) m/z 889.7; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI-FTMS, [M+H]1+), 445.21312.


Example 8DDDDDD
N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-N-(2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 457.4; MS (ESI) m/z 913.7; HRMS: calcd for C28H28N2O4+H+, 457.21218; found (ESI-FTMS, [M+H]1+), 457.21146.


Example 8EEEEEE
N2-(biphenyl-4-ylcarbonyl)-N-butyl-N-methyl-L-α-glutamine

MS (ESI) m/z 397.2; MS (ESI) m/z 793.4; MS (ESI) m/z 419.2; HRMS: calcd for C23H28N2O4+H+, 397.21218; found (ESI-FTMS, [M+H]1+), 397.21249.


Example 8FFFFFF
N-butyl-N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-L-α-glutamine

MS (ESI) m/z 409.3; MS (ESI) m/z 817.5; HRMS: calcd for C24H28N2O4+H+, 409.21218; found (ESI-FTMS, [M+H]1+), 409.21227.


Example 8GGGGGG
N2-(biphenyl-4-ylcarbonyl)-N-[2-(3,4-dimethoxyphenyl)ethyl]-N-methyl-L-α-glutamine

MS (ESI) m/z 505.3; MS (ESI) m/z 527.3; MS (ESI) m/z 1009.6; HRMS: calcd for C29H32N2O6+H+, 505.23331; found (ESI-FTMS, [M+H]1+), 505.23512.


Example 8HHHHHH
N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 521.1; MS (ESI) m/z 1041.3; HRMS: calcd for C29H29FN2O6+H+, 521.20824; found (ESI-FTMS, [M+H]1+), 521.20872.


Example 8IIIIII
N-[2-(3,4-dimethoxyphenyl)ethyl]-N2-(9H-fluoren-2-ylcarbonyl)-N-methyl-L-α-glutamine

MS (ESI) m/z 517.2; MS (ESI) m/z 1033.3; HRMS: calcd for C30H32N2O6+H+, 517.23331; found (ESI-FTMS, [M+H]1+), 517.23342.


Example 8JJJJJJ
N2-(9H-fluoren-2-ylcarbonyl)-N-[(2R)-2-phenylpropyl]-L-α-glutamine

MS (ESI) m/z 457.2; MS (ESI) m/z 913.3; MS (ESI) m/z 479.1; HRMS: calcd for C28H28N2O4+H+, 457.21218; found (ESI-FTMS, [M+H]1+), 457.21246.


Example 8KKKKKK
N2-(biphenyl-4-ylcarbonyl)-N-[(2R)-2-phenylpropyl]-L-α-glutamine

MS (ESI) m/z 445.2; MS (ESI) m/z 889.3; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI-FTMS, [M+H]1+), 445.21285.


Example 8LLLLLL
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamamide

MS (ESI) m/z 476.2; MS (ESI) m/z 951.5; HRMS: calcd for C28H30FN3O3+H+, 476.23440; found (ESI-FTMS, [M+H]1+), 476.23543.


Example 8MMMMMM
N-[(1S)-1-benzyl-2-hydroxyethyl]-N2-(biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 461.2; MS (ESI) m/z 921.4; MS (ESI) m/z 943.4; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI-FTMS, [M+H]1+), 461.20748.


Example 8NNNNNN
N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutamine

MS (ESI) m/z 419.1; MS (ESI) m/z 837.2; HRMS: calcd for C19H19BrN2O4+H+, 419.06009; found (ESI_FT, [M+H]1+), 419.05998.


Example 8OOOOOO
N1-[2-(acetylamino)ethyl]-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 410.2; MS (ESI) m/z 821.5; HRMS: calcd for C22H25N3O5+H+, 412.18670; found (ESI_FT, [M+H]1+), 412.18628.


Example 8PPPPPP
N2-(1,1′-biphenyl-4-ylacetyl)-N1-(3-methoxybenzyl)-L-α-glutamine

The title compound was prepared according to procedures similar to those described in Example 7, except biphenylacetic acid was used. MS (ESI) m/z 461.2; MS (ESI) m/z 921.4; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FTMS, [M+H]1+), 461.20514.


Example 8QQQQQQ
N1-(2-benzylphenyl)-N2-(1,1′-biphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI−) m/z 491.3; HRMS: calcd for C31H28N2O4+H+, 493.21218; found (ESI-FTMS, [M+H]1+), 493.21319.


Example 8RRRRRR
N2-2-naphthoyl-N1-(3-phenylpropyl)-L-α-glutamine

MS (ESI) m/z 419.1; MS (ESI) m/z 837.1; MS (ESI) m/z 441.1; HRMS: calcd for C25H26N2O4+H+, 419.19653; found (ESI-FTMS, [M+H]1+), 419.19678.


Example 8SSSSSS
N2-[(9-oxo-9H-fluoren-2-yl)carbonyl]-N1-(3-phenylpropyl)-L-α-glutamine

MS (ESI) m/z 471.3; MS (ESI) m/z 941.5; HRMS: calcd for C28H26N2O5+H+, 471.19145; found (ESI-FTMS, [M+H]1+), 471.19142.


Example 8TTTTTT
N2-(4-phenoxybenzoyl)-N1-(3-phenylpropyl)-L-α-glutamine

HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI-FTMS, [M+H]1+), 461.20673.


Example 8UUUUUU
N1-benzyl-N2-[4-(pyrimidin-2-ylamino)benzoyl]-L-α-glutamine

MS m/z 02-101699GMS.


Example 8VVVVVV
N2-(9H-fluoren-2-ylcarbonyl)-N1-(6-hydroxyhexyl)-L-α-glutamine

MS (ESI) m/z 439.2; HRMS: calcd for C25H30N2O5+H+, 439.22275; found (ESI-FTMS, [M+H]1+), 439.22379.


Example 8WWWWWW
N2-[4-(5-bromo-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 561.3; MS (ESI) m/z 1121.5; HRMS: calcd for C26H26BrFN2O4S+H+, 561.08534; found (ESI-FTMS, [M+H]1+), 561.08472.


Example 8XXXXXX
N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 517.3; MS (ESI) m/z 1033.5; MS (ESI) m/z 539.3; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13517.


MS (ESI) m/z 517.3; MS (ESI) m/z 1033.6; MS (ESI) m/z 539.3; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13686.


Example 8YYYYYY
N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 513.4; MS (ESI) m/z 1025.7; MS (ESI) m/z 535.3; HRMS: calcd for C28H27F3N2O4+H+, 513.19957; found (ESI-FTMS, [M+H]1+), 513.19837.


MS (ESI) m/z 513.4; MS (ESI) m/z 1025.8; MS (ESI) m/z 535.4; HRMS: calcd for C28H27F3N2O4+H+, 513.19957; found (ESI-FTMS, [M+H]1+), 513.20152.


Example 8ZZZZZZ
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.5; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.16931.


Example 8AAAAAAA
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.5; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.16951.


Example 8BBBBBBB
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,5′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 529.3; MS (ESI) m/z 1057.5; MS (ESI) m/z 551.2; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.16954.


Example 8CCCCCCC
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,6′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.4; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.16943.


Example 8DDDDDDD
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(3′,5′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 529.3; MS (ESI) m/z 1057.5; MS (ESI) m/z 551.3; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.17191.


Example 8EEEEEEE
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(2′,3′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.17167.


Example 8FFFFFFF
N-(1,1-dimethyl-2-phenylethyl)-N2-(4-pyrimidin-5-ylbenzoyl)-L-α-glutamine

MS (ESI) m/z 461.2; MS (ESI) m/z 921.4; HRMS: calcd for C26H28N4O4+H+, 461.21833; found (ESI-FTMS, [M+H]1+), 461.21863.


Example 8GGGGGGG
N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 495.2; MS (ESI) m/z 989.4; HRMS: calcd for C28H28F2N2O4+H+, 495.20899; found (ESI-FTMS, [M+H]1+), 495.21035.


Example 8HHHHHHH
N2-[(3′,5′-difluorobiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 495.2; MS (ESI) m/z 989.4; MS (ESI) m/z 517.2; HRMS: calcd for C28H28F2N2O4+H+, 495.20899; found (ESI-FTMS, [M+H]1+), 495.21017.


Example 8IIIIIII
N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-N2-[(3′,4′-difluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 533.3; MS (ESI) m/z 1067.7; HRMS: calcd for C26H25ClF2N2O4S+H+, 535.12644; found (ESI-FTMS, [M+H]1+), 535.1267.


Example 8JJJJJJJ
N2-(biphenyl-4-ylcarbonyl)-N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 499.4; MS (ESI) m/z 997.7; MS (ESI) m/z 521.4; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14616.


Example 8KKKKKKK
N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(5-chloro-2-thienyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 557.3; MS (ESI) m/z 1113.6; HRMS: calcd for C24H23Cl2FN2O4S2+H+, 557.05331; found (ESI-FTMS, [M+H]1+), 557.05439.


Example 8LLLLLLL
N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 537.1; MS (ESI) m/z 1073.3; HRMS: calcd for C29H32N2O8+H+, 537.22314; found (ESI-FTMS, [M+H]1+), 537.22326.


Example 8MMMMMMM
N2-{[4′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 599.1; MS (ESI) m/z 1197.1; HRMS: calcd for C29H31BrN2O7+H+, 599.13874; found (ESI-FTMS, [M+H]1+), 599.13934.


Example 8NNNNNNN
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 483.3; MS (ESI) m/z 965.5; HRMS: calcd for C26H27FN2O4S+H+, 483.17483; found (ESI-FTMS, [M+H]1+), 483.1743.


Example 8OOOOOOO
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(2-furyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 467.3; MS (ESI) m/z 933.6; HRMS: calcd for C26H27FN2O5+H+, 467.19768; found (ESI-FTMS, [M+H]1+), 467.19704.


Example 8PPPPPPP
N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 459.1; MS (ESI) m/z 919.5; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI-FTMS, [M+H]1+), 461.20822.


Example 8QQQQQQQ
N2-{[3′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 459.2; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI-FTMS, [M+H]1+), 461.20807.


Example 8RRRRRRR
N2-[4-(2,3-dihydro-1-benzofuran-5-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 517.3; MS (ESI) m/z 1035.7; HRMS: calcd for C30H31FN2O5+H+, 519.22898; found (ESI-FTMS, [M+H]1+), 519.22989.


Example 8SSSSSSS
N2-[(3′-chloro-4′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 529.3; MS (ESI) m/z 1057.6; HRMS: calcd for C28H27ClF2N2O4+H+, 529.17002; found (ESI-FTMS, [M+H]1+), 529.17061.


Example 8TTTTTTT
N2-{[3′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 521.1; HRMS: calcd for C27H27BrN2O4+H+, 523.12270; found (ESI-FTMS, [M+H]1+), 523.12235.


Example 8UUUUUUU
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(3-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 483.1; MS (ESI) m/z 965.3; HRMS: calcd for C26H27FN2O4S+H+, 483.17483; found (ESI-FTMS, [M+H]1+), 483.17572.


Example 8VVVVVVV
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[4-(5-methyl-2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 495.4; MS (ESI) m/z 991.7; HRMS: calcd for C27H29FN2O4S+H+, 497.19048; found (ESI-FTMS, [M+H]1+), 497.19118.


Example 8WWWWWWW
N2-[4-(5-chloro-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 515.3; MS (ESI) m/z 1031.7; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13644.


MS (ESI) m/z 515.3; MS (ESI) m/z 1031.6; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13658.


Example 8XXXXXXX
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 497.3; MS (ESI) m/z 995.6; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14509.


Example 8YYYYYYY
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 529.3; MS (ESI) m/z 1057.5; HRMS: calcd for C27H29ClN2O5S+H+, 529.15585; found (ESI-FTMS, [M+H]1+), 529.15608.


Example 8ZZZZZZZ
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 499.2; MS (ESI) m/z 997.5; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14644.


Example 8AAAAAAAA
N2-(2,2′-bithien-5-ylcarbonyl)-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 505.1; MS (ESI) m/z 1009.1; HRMS: calcd for C24H25ClN2O4S2+H+, 505.10170; found (ESI-FTMS, [M+H]1+), 505.10282.


Example 8BBBBBBBB
N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 517.5; MS (ESI) m/z 1035.9; HRMS: calcd for C30H31FN2O5+H+, 519.22898; found (ESI-FTMS, [M+H]1+), 519.22971.


Example 8CCCCCCCC
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[6-(3-methoxyphenyl)pyridin-3-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 508.4; HRMS: calcd for C28H30FN3O5+H+, 508.22423; found (ESI-FTMS, [M+H]1+), 508.22472.


Example 8DDDDDDDD
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 478.4; HRMS: calcd for C27H28FN3O4+H+, 478.21366; found (ESI-FTMS, [M+H]1+), 478.21435.


Example 8EEEEEEEE
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 483.2; MS (ESI) m/z 965.4; HRMS: calcd for C26H27FN2O4S+H+, 483.17483; found (ESI-FTMS, [M+H]1+), 483.17576.


Example 8FFFFFFFF
N2-[(3-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 495.3; MS (ESI) m/z 989.6; HRMS: calcd for C28H28F2N2O4+H+, 495.20899; found (ESI-FTMS, [M+H]1+), 495.20963.


Example 8GGGGGGGG
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-4-yl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 500.3; HRMS: calcd for C25H26ClN3O4S+H+, 500.14053; found (ESI-FTMS, [M+H]1+), 500.13984.


Example 8HHHHHHHH
N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 549.3; HRMS: calcd for C26H25Cl2FN2O4S+H+, 551.09689; found (ESI-FTMS, [M+H]1+), 551.09834.


Example 8IIIIIIII
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 535.4; MS (ESI) m/z 1069.8; HRMS: calcd for C30H31ClN2O5+H+, 535.19943; found (ESI-FTMS, [M+H]1+), 535.20047.


Example 8JJJJJJJJ
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 500.3; HRMS: calcd for C25H26ClN3O4S+H+, 500.14053; found (ESI-FTMS, [M+H]1+), 500.1423.


Example 8KKKKKKKK
N2-[4-(2,3-dihydro-1-benzofuran-6-yl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 501.4; MS (ESI) m/z 1001.8; HRMS: calcd for C30H32N2O5+H+, 501.23840; found (ESI-FTMS, [M+H]1+), 501.23954.


Example 8LLLLLLLL
N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 515.3; MS (ESI) m/z 1031.7; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13443.


Example 8MMMMMMMM
N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 465.2; MS (ESI) m/z 929.4; HRMS: calcd for C26H28N2O4S+H+, 465.18425; found (ESI-FTMS, [M+H]1+), 465.18402.


Example 8NNNNNNNN
N-(1,1-dimethyl-2-phenylethyl)-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 495.2; MS (ESI) m/z 989.4; HRMS: calcd for C27H30N2O5S+H+, 495.19482; found (ESI-FTMS, [M+H]1+), 495.1942.


Example 8OOOOOOOO
N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-phenyl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 465.2; MS (ESI) m/z 929.4; HRMS: calcd for C26H28N2O4S+H+, 465.18425; found (ESI-FTMS, [M+H]1+), 465.18359.


Example 8PPPPPPPP
N2-(2,2′-bithien-5-ylcarbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 469.3; MS (ESI) m/z 939.6; HRMS: calcd for C24H26N2O4S2+H+, 471.14067; found (ESI-FTMS, [M+H]1+), 471.1403.


Example 8QQQQQQQQ
N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-pyridin-4-yl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 466.4; HRMS: calcd for C25H27N3O4S+H+, 466.17950; found (ESI-FTMS, [M+H]1+), 466.17836.


Example 8RRRRRRRR
N-(1,1-dimethyl-2-phenylethyl)-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 466.3; HRMS: calcd for C25H27N3O4S+H+, 466.17950; found (ESI-FTMS, [M+H]1+), 466.17912.


Example 8SSSSSSSS
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(5-chloro-2-thienyl)benzoyl]-L-α-glutamine

HRMS: calcd for C26H26Cl2N2O4S+H+, 533.10631; found (ESI-FTMS, [M+H]1+), 533.10629.


Example 8TTTTTTTT
N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 500.3; MS (ESI) m/z 999.6; HRMS: calcd for C25H26ClN3O4S+H+, 500.14053; found (ESI-FTMS, [M+H]1+), 500.14123.


Example 8UUUUUUUU
N-(1,1-dimethyl-2-phenylethyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 460.4; HRMS: calcd for C27H29N3O4+H+, 460.22308; found (ESI-FTMS, [M+H]1+), 460.22319.


Example 8VVVVVVVV
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-(5-phenyl-2-furoyl)-L-α-glutamine

MS (ESI) m/z 483.3; MS (ESI) m/z 965.6; MS (ESI) m/z 987.6; HRMS: calcd for C26H27ClN2O5+H+, 483.16813; found (ESI-FTMS, [M+H]1+), 483.16818.


Example 8WWWWWWWW
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 534.3; HRMS: calcd for C25H25Cl2N3O4S+H+, 534.10156; found (ESI-FTMS, [M+H]1+), 534.10123.


Example 8XXXXXXXX
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 494.4; HRMS: calcd for C27H28ClN3O4+H+, 494.18411; found (ESI-FTMS, [M+H]1+), 494.18423.


Example 8YYYYYYYY
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[5-(3-methoxyphenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 513.3; MS (ESI) m/z 1025.7; HRMS: calcd for C27H29FN2O5S+H+, 513.18540; found (ESI-FTMS, [M+H]1+), 513.18635.


Example 8ZZZZZZZZ
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(5-pyridin-3-yl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 484.3; HRMS: calcd for C25H26FN3O4S+H+, 484.17008; found (ESI-FTMS, [M+H]1+), 484.17109.


Example 8AAAAAAAAA
N2-{[5-(3-chloro-4-fluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 535.2; MS (ESI) m/z 557.2; MS (ESI) m/z 1069.5; HRMS: calcd for C26H25ClF2N2O4S+H+, 535.12644; found (ESI-FTMS, [M+H]1+), 535.12696.


MS (ESI) m/z 533.3; MS (ESI) m/z 1067.6; HRMS: calcd for C26H25ClF2N2O4S+H+, 535.12644; found (ESI-FTMS, [M+H]1+), 535.12796.


Example 8BBBBBBBBB
N2-(2,2′-bithien-5-ylcarbonyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 489.3; MS (ESI) m/z 977.5; HRMS: calcd for C24H25FN2O4S2+H+, 489.13125; found (ESI-FTMS, [M+H]1+), 489.13208.


Example 8CCCCCCCCC
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(5-formyl-2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 527.1; MS (ESI) m/z 1053.1; MS (ESI) m/z 549; HRMS: calcd for C27H27ClN2O5S+H+, 527.14020; found (ESI-FTMS, [M+H]1+), 527.14185.


Example 8DDDDDDDDD
N2-[4-(5-acetyl-2-thienyl)benzoyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 541.1; MS (ESI) m/z 1081.2; MS (ESI) m/z 563.1; HRMS: calcd for C28H29ClN2O5S+H+, 541.15585; found (ESI-FTMS, [M+H]1+), 541.15471.


Example 8EEEEEEEEE
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 500.3; HRMS: calcd for C25H26ClN3O4S+H+, 500.14053; found (ESI-FTMS, [M+H]1+), 500.14163.


Example 8FFFFFFFFF
N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 466.3; HRMS: calcd for C25H27N3O4S+H+, 466.17950; found (ESI-FTMS, [M+H]1+), 466.18052.


Example 8GGGGGGGGG
N-(1,1-dimethyl-2-phenylethyl)-N2-(5-phenyl-2-furoyl)-L-α-glutamine

MS (ESI) m/z 449.3; MS (ESI) m/z 897.6; MS (ESI) m/z 471.3; HRMS: calcd for C26H28N2O5+H+, 449.20710; found (ESI-FTMS, [M+H]1+), 449.20772.


Example 8HHHHHHHHH
N-(1,1-dimethyl-2-phenylethyl)-N2-[4-(5-formyl-2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 493.3; MS (ESI) m/z 985.6; MS (ESI) m/z 515.3; HRMS: calcd for C27H28N2O5S+H+, 493.17917; found (ESI-FTMS, [M+H]1+), 493.18011.


Example 8IIIIIIIII
N2-[4-(5-acetyl-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 507.3; MS (ESI) m/z 1013.7; MS (ESI) m/z 529.3; HRMS: calcd for C28H30N2O5S+H+, 507.19482; found (ESI-FTMS, [M+H]1+), 507.19518.


Example 8JJJJJJJJJ
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[3-(5-chloro-2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 533.3; MS (ESI) m/z 1065.5; MS (ESI) m/z 555.3; HRMS: calcd for C26H26Cl2N2O4S+H+, 533.10631; found (ESI-FTMS, [M+H]1+), 533.10835.


Example 8KKKKKKKKK
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-[3-(2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 499.3; MS (ESI) m/z 997.6; MS (ESI) m/z 521.3; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14679.


Example 8LLLLLLLLL
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 533.3; MS (ESI) m/z 1065.5; MS (ESI) m/z 555.3; HRMS: calcd for C26H26Cl2N2O4S+H+, 533.10631; found (ESI-FTMS, [M+H]1+), 533.10784.


Example 8MMMMMMMMM
N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 517.3; MS (ESI) m/z 539.3; MS (ESI) m/z 1033.6; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.13691.


Example 8NNNNNNNNN
N2-[4-(5-bromo-2-thienyl)benzoyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 577.2; MS (ESI) m/z 1153.4; HRMS: calcd for C26H26BrClN2O4S+H+, 577.05579; found (ESI-FTMS, [M+H]1+), 577.05597.


Example 8OOOOOOOOO
N2-[4-(5-bromo-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 543.1; MS (ESI) m/z 1085.2; HRMS: calcd for C26H27BrN2O4S+H+, 543.09477; found (ESI-FTMS, [M+H]1+), 543.0965; HRMS: calcd for C26H27BrN2O4S+H+, 543.09477; found (ESI-FTMS, [M+H]1+), 543.09494.


Example 8PPPPPPPPP
N-(1,1-dimethyl-2-phenylethyl)-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 483.2; MS (ESI) m/z 965.4; MS (ESI) m/z 505.2; HRMS: calcd for C26H27FN2O4S+H+, 483.17483; found (ESI-FTMS, [M+H]1+), 483.17634.


Example 8QQQQQQQQQ
N2-[3-(5-chloro-2-thienyl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 499.2; MS (ESI) m/z 997.3; MS (ESI) m/z 521.1; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14544.


Example 8RRRRRRRRR
N-(1,1-dimethyl-2-phenylethyl)-N2-[3-(2-thienyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 465.2; MS (ESI) m/z 929.4; MS (ESI) m/z 487.2; HRMS: calcd for C26H28N2O4S+H+, 465.18425; found (ESI-FTMS, [M+H]1+), 465.18439.


Example 8SSSSSSSSS
N2-[(3′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

HRMS: calcd for C28H28ClFN2O4+H+, 511.17944; found (ESI-FTMS, [M+H]1+), 511.17974.


Example 8TTTTTTTTT
N2-[(4′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

HRMS: calcd for C28H28ClFN2O4+H+, 511.17944; found (ESI-FTMS, [M+H]1+), 511.17972.


Example 8UUUUUUUUU
N2-[(4′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

HRMS: calcd for C28H28F2N2O4+H+, 495.20899; found (ESI-FTMS, [M+H]1+), 495.20933.


Example 8VVVVVVVVV
N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

HRMS: calcd for C28H28F2N2O4+H+, 495.20899; found (ESI-FTMS, [M+H]1+), 495.20937.


Example 8WWWWWWWWW
N-[2-(4-chloro phenyl)-1,1-dimethylethyl]-N2-[(3-phenyl-2-thienyl)carbonyl]-L-α-glutamine

HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14664.


Example 8XXXXXXXXX
N2-[(4′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 527.3; MS (ESI) m/z 1053.7; MS (ESI) m/z 549.3; HRMS: calcd for C28H28Cl2N2O4+H+, 527.14989; found (ESI-FTMS, [M+H]1+), 527.15049.


HRMS: calcd for C28H28Cl2N2O4+H+, 527.14989; found (ESI-FTMS, [M+H]1+), 527.14931.


Example 8YYYYYYYYY
N2-[(3′-chlorobiphenyl-4-yl)carbonyl]-N-[2-(4-chlorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 527.3; MS (ESI) m/z 1053.7; MS (ESI) m/z 549.3; HRMS: calcd for C28H28Cl2N2O4+H+, 527.14989; found (ESI-FTMS, [M+H]1+), 527.15048.


Example 8ZZZZZZZZZ
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-{[5-(4-fluorophenyl)-2-thienyl]carbonyl}-L-α-glutamine

MS (ESI) m/z 501.3; MS (ESI) m/z 1001.6; MS (ESI) m/z 523.3; HRMS: calcd for C26H26F2N2O4S+H+, 501.16541; found (ESI-FTMS, [M+H]1+), 501.16642.


MS (ESI) m/z 501.3; MS (ESI) m/z 1001.6; MS (ESI) m/z 523.3; HRMS: calcd for C26H26F2N2O4S+H+, 501.16541; found (ESI-FTMS, [M+H]1+), 501.16709.


Example 8AAAAAAAAAA
N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 499.1; MS (ESI) m/z 997.2; HRMS: calcd for C26H27ClN2O4S+H+, 499.14528; found (ESI-FTMS, [M+H]1+), 499.14552.


Example 8BBBBBBBBBB
N-(1,1-dimethyl-2-phenylethyl)-N2-[(3-phenyl-2-thienyl)carbonyl]-L-α-glutamine

MS (ESI) m/z 465.2; MS (ESI) m/z 929.4; MS (ESI) m/z 951.4; HRMS: calcd for C26H28N2O4S+H+, 465.18425; found (ESI-FTMS, [M+H]1+), 465.18455.


Example 8CCCCCCCCCC
N2-(4-bromobenzoyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 479.3; MS (ESI) m/z 957.5; MS (ESI) m/z 501.2; HRMS: calcd for C22H24BrFN2O4+H+, 479.09762; found (ESI-FTMS, [M+H]1+), 479.0983.


Example 8DDDDDDDDDD
N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 477.3; MS (ESI) m/z 499.3; MS (ESI) m/z 953.7; HRMS: calcd for C28H29FN2O4+H+, 477.21841; found (ESI-FTMS, [M+H]1+), 477.21891.


Example 8EEEEEEEEEE
N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-fluorobiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 477.3; MS (ESI) m/z 499.3; MS (ESI) m/z 953.7; HRMS: calcd for C28H29FN2O4+H+, 477.21841; found (ESI-FTMS, [M+H]1+), 477.21875.


Example 8FFFFFFFFFF
N2-[4-(5-chloro-2-thienyl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-D-α-glutamine

MS (ESI) m/z 517.2; MS (ESI) m/z 539.2; MS (ESI) m/z 1033.5; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.1365.


Example 8GGGGGGGGGG
N2-{[6-(5-chloro-2-thienyl)pyridin-3-yl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 518.2; HRMS: calcd for C25H25ClFN3O4S+H+, 518.13111; found (ESI-FTMS, [M+H]1+), 518.13169.


Example 8HHHHHHHHHH
N2-{[5-(4-chlorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 517.3; MS (ESI) m/z 539.3; MS (ESI) m/z 1033.6; HRMS: calcd for C26H26ClFN2O4S+H+, 517.13586; found (ESI-FTMS, [M+H]1+), 517.1363.


Example 8IIIIIIIIII
N2-{[5-(3,4-difluorophenyl)-2-thienyl]carbonyl}-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 519.3; MS (ESI) m/z 1037.7; MS (ESI) m/z 541.3; HRMS: calcd for C26H25F3N2O4S+H+, 519.15599; found (ESI-FTMS, [M+H]1+), 519.1568.


The following intermediates (R1COOH in scheme 7) were prepared and used in the preparation of some of the compounds of Example 8:


Example 8JJJJJJJJJJ
4-(2-thienyl)benzoic acid






4-iodobenzoic acid (3 g, 12.1 mmol), 2-thiopheneboronic acid (3.10 g, 24.2 mmol), and Pd(PPh3)4 (1.4 g, 1.21 mmol) were dissolved in DMF (75 mL) in a 350 mL screw-cap pressure vessel. In a separate flask, K2CO3 (5.02 g, 36.3 mmol) was dissolved in H2O (12 mL) and was then added to the reaction. The flask was sealed, heated to 100° C. and stirred overnight (17 h).


The reaction was then diluted with H2O (150 mL), placed in a sep. funnel, and 1N NaOH was added to raise the pH to 10. The aqueous layer was washed with EtOAc (2×250 mL). The aqueous layer was kept, activated charcoal was added, and the mixture heated and stirred. Celite was added, followed by the mixture being filtered through celite. The filtrate was then acidified with concentrated HCl. At pH 7 a tan solid starts to precipitate. The pH was lowered to 5 and the solid formed is filtered out. The solid was partially dissolved in a small amount of toluene, which was then removed to remove some of the water still present. This step was repeated, then the solid was dried under vacuum. Yield—2.01 g tan solid, 81%. MS (ESI) m/z 202.9; HRMS: calcd for C11H8O2S+H+, 205.03178; found (ESI-FTMS, [M+H]1+), 205.03127.


The following compounds were prepared according to the procedure similar to the one described in Example 8JJJJJJJJJJ, from appropriate commercially available reagents:


i: 5-(3-methoxyphenyl)thiophene-2-carboxylic acid

MS (ESI) m/z 233.1; HRMS: calcd for C12H10O3S+H+, 235.04234; found (ESI-FTMS, [M+H]1+), 235.04226;


ii. 2,2′-bithiophene-5-carboxylic acid

MS (ESI) m/z 209.1; HRMS: calcd for C9H6O2S2+H+, 210.98820; found (ESI-FTMS, [M+H]1+), 210.98816;


iii. 5-pyridin-4-ylthiophene-2-carboxylic acid

MS (ESI) m/z 206.1; HRMS: calcd for C10H7NO2S+H+, 206.02703; found (ESI-FTMS, [M+H]1+), 206.02691;


iv. 4-(5-chloro-2-thienyl)benzoic acid

HRMS: calcd for C11H7ClO2S−H+, 236.97825; found (ESI-FTMS, [M−H]1−), 236.97751;


v. 5-phenyl-2-furoic acid

HRMS: calcd for C11H8O3+H+, 189.05462; found (ESI-FTMS, [M+H]1+), 189.05445;


vi. 4-(5-acetyl-2-thienyl)benzoic acid

MS (ESI) m/z 245.1; HRMS: calcd for C13H10O3S+H+, 247.04234; found (ESI-FTMS, [M+H]1+), 247.04223;


vii. 3-(5-chloro-2-thienyl)benzoic acid

MS (ESI) m/z 237; HRMS: calcd for C11H7ClO2S−H+, 236.97825; found (ESI-FTMS, [M−H]1−), 236.97757;


viii. 3-(2-thienyl)benzoic acid

MS (ESI) m/z 203.1; HRMS: calcd for C11H8O2S+H+, 205.03178; found (ESI-FTMS, [M+H]1+), 205.03194;


ix. 5-(4-chlorophenyl)thiophene-2-carboxylic acid

MS (ESI) m/z 237; MS (ESI) m/z 283; MS (ESI) m/z 475.1; HRMS: calcd for C11H7ClO2S−H+, 236.97825; found (ESI-FTMS, [M−H]1−), 236.97825;


x. 3-phenylthiophene-2-carboxylic acid

MS (ESI) m/z 203.1; MS (ESI) m/z 249.1; HRMS: calcd for C11H8O2S+H+, 205.03178; found (ESI-FTMS, [M+H]1+), 205.03177;


xi. 4′-chlorobiphenyl-4-carboxylic acid

MS (ESI) m/z 231.1; HRMS: calcd for C13H9ClO2−H+, 231.02183; found (ESI-FTMS, [M−H]1−), 231.02129;


xii. 3′-chlorobiphenyl-4-carboxylic acid

MS (ESI) m/z 231.1; HRMS: calcd for C13H9ClO2−H+, 231.02183; found (ESI-FTMS, [M−H]1−), 231.02128;


xiii. 5-(3,4-difluorophenyl)thiophene-2-carboxylic acid

MS (ESI) m/z 239; MS (ESI) m/z 285.1; MS (ESI) m/z 479.1; HRMS: calcd for C11H6F2O2S+H+, 241.01293; found (ESI-FTMS, [M+H]1+), 241.01183;


xiv. 5-(2,4-difluorophenyl)thiophene-2-carboxylic acid

MS (ESI) m/z 239; MS (ESI) m/z 285; HRMS: calcd for C11H6F2O2S+H+, 241.01293; found (ESI-FTMS, [M+H]1+), 241.01187;


xv. 4-phenoxybenzoic acid

MS (ESI) m/z 213.1; HRMS: calcd for C13H10O3+H+, 215.07027; found (ESI-FTMS, [M+H]1+), 215.07029;


xvi. 4-(2,3-dihydro-1-benzofuran-6-yl)benzoic acid

MS (ESI) m/z 239.1; HRMS: calcd for C15H12O3−H+, 239.07137; found (ESI-FTMS, [M−H]1−), 239.07131;


xvii. 5-(3-chloro-4-fluorophenyl)thiophene-2-carboxylic acid

MS (ESI) m/z 255.1; MS (ESI) m/z 511.2; HRMS: calcd for C11H6ClFO2S−H+, 254.96883; found (ESI-FTMS, [M−H]1−), 254.96892;


xviii. 5-pyridin-3-ylthiophene-2-carboxylic acid

MS (ESI) m/z 206.1; HRMS: calcd for C10H7NO2S+H+, 206.02703; found (ESI-FTMS, [M+H]1+), 206.02708;


xix. 6-(5-chloro-2-thienyl)nicotinic acid

MS (ESI) m/z 238;


xx. 6-phenylnicotinic acid

MS (ESI) m/z 200.1; HRMS: calcd for C12H9NO2+H+, 200.07061; found (ESI-FTMS, [M+H]1+), 200.07052;


xxi. 4-(1,3-thiazol-2-yl)benzoic acid

mp 235-237° C.; MS (ESI) m/z 206; MS (ESI) m/z 204;


xxii. 4-(5-bromo-2-thienyl)benzoic acid

MS (ESI) m/z 281; MS (ESI) m/z 394.8;


xxiii. 5-(4-fluorophenyl)thiophene-2-carboxylic acid

MS (ES) m/z 221.0; MS (ES) m/z 443.0;


xxiv. 4-[5-(aminosulfonyl)-2-thienyl]benzoic acid

MS (ESI) m/z 282.1; MS (ESI) m/z 565.2;


xxv. 4-(5-cyano-2-thienyl)benzoic acid

MS (ESI) m/z 228.1;


xxvi. 3′-fluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 215.1;


xxvii. 3′,4′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233;


xxviii. 2′,4′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233.1;


xxix. 2′,5′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233.1;


xxx. 3′,5′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233.1;


xxxi. 2′,6′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233.1;


xxxii. 2′,3′-difluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 233;


xxxiii. 4-(5-methyl-2-thienyl)benzoic acid

MS (ESI) m/z 217.1; HRMS: calcd for C12H10O2S+H+, 219.04743; found (ESI-FTMS, [M+H]1+), 219.04744;


xxxiv. 6-(3-methoxyphenyl)nicotinic acid

MS (ESI) m/z 230.1;


xxxv. 3-fluorobiphenyl-4-carboxylic acid

MS (ESI) m/z 215.1; HRMS: calcd for C13H9FO2+H+, 217.06593; found (ESI-FTMS, [M+H]1+), 217.06598.







Example 9
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutaminate

tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-bromophenyl)ethyl]-L-α-glutaminate (333 mg, 0.59 mmol, 1 equiv.) was dissolved in DME (5 mL) and the mixture was added to 4-formylphenyl boronic acid (0.97 g, 0.65 mmol, 1.1 equiv.) and Pd(PPh3)4 (68 mg, 0.059 mmol, 0.1 equiv.) and stirred for 30 minutes prior to the addition of aq. K2CO3 (163 mg, 1.18 mmol, 2 equiv.) in 1 mL H2O. The mixture was capped in a sealed vessel and stirred overnight at 80° C. The reaction was complete as determined by TLC. The mixture was filtered over celite, the solvent was removed, and the resulting tan solid was diluted with EtOAc (20 mL) and washed consecutively with H2O, 10% HCl, brine, and dried over Na2SO4. The solid was purified by column chromatography (silica gel, 41% Acetone/Hexanes) to give 38 mg of tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutaminate in 11% yield. MS (ESI) m/z 591.3; MS (ESI) m/z 1181.5; HRMS: calcd for C37H38N2O5+H+, 591.28535; found (ESI-FTMS, [M+H]1+), 591.28473.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutamine

tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutaminate (100 mg, 0.17 mmol, 1 equiv.) was dissolved in CH2Cl2 (5 mL) and added to TFA (2 mL). The mixture was stirred at room temperature for 3 hrs. The reaction was complete as determined by TLC. Solvent was removed and the resulting solid was purified by preparative HPLC to give N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-4-yl)ethyl]-L-α-glutamine in 89% yield (80 mg). MS (ESI) m/z 533.1; HRMS: calcd for C33H30N2O5+H+, 535.22275; found (ESI-FTMS, [M+H]1+), 535.22464.


Example 10

The following compounds were prepared according to procedures similar to those described in Example 9.


Example 10A
N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-4-yl]ethyl}-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-4-yl]ethyl}-L-α-glutaminate

MS (ESI) m/z 631.2; MS (ESI) m/z 1261.4; MS (ESI) m/z 653.2; HRMS: calcd for C37H37F3N2O4+H+, 631.27782; found (ESI-FTMS, [M+H]1+), 631.27719.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-4-yl]ethyl}-L-α-glutamine

HRMS: calcd for C33H29F3N2O4+H+, 575.21522; found (ESI-FTMS, [M+H]1+), 575.21548.


Example 10B
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-4-yl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-4-yl)ethyl]-L-α-glutaminate

MS (ESI) m/z 593.3; MS (ESI) m/z 1185.5; HRMS: calcd for C37H40N2O5+H+, 593.30100; found (ESI-FTMS, [M+H]1+), 593.29946.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-4-yl)ethyl]-L-α-glutamine

HRMS: calcd for C33H32N2O5+H+, 537.23840; found (ESI-FTMS, [M+H]1+), 537.23906.


Example 10C
N2-(biphenyl-4-ylcarbonyl)-N1-(2-biphenyl-4-ylethyl)-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(2-biphenyl-4-ylethyl)-L-α-glutaminate

MS (ESI) m/z 563.3; MS (ESI) m/z 1125.5; HRMS: calcd for C36H38N2O4+H+, 563.29043; found (ESI-FTMS, [M+H]1+), 563.28977.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-(2-biphenyl-4-ylethyl)-L-α-glutamine

HRMS: calcd for C32H30N2O4+H+, 507.22783; found (ESI-FTMS, [M+H]1+), 507.22688.


Example 10D
N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4-(2-thienyl)phenyl]ethyl}-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4-(2-thienyl)phenyl]ethyl}-L-α-glutaminate

MS (ESI) m/z 569.2; MS (ESI) m/z 1137.4; HRMS: calcd for C34H36N2O4S+H+, 569.24685; found (ESI-FTMS, [M+H]1+), 569.24702.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4-(2-thienyl)phenyl]ethyl}-L-α-glutamine

MS (ESI) m/z 511.1; HRMS: calcd for C30H28N2O4S+H+, 513.18425; found (ESI-FTMS, [M+H]1+), 513.18417.


Example 10E
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(2′-ethoxybiphenyl-4-yl)ethyl]-L-α-glutamine

MS (ESI) m/z 551.1; MS (ESI) m/z 1101.4; MS (ESI) m/z 573.1; HRMS: calcd for C34H34N2O5+H+, 551.25405; found (ESI-FTMS, [M+H]1+), 551.2538.


Example 10F
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-ethynylbiphenyl-4-yl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-ethynylbiphenyl-4-yl)ethyl]-L-α-glutaminate

MS (ESI) m/z 587.3; MS (ESI) m/z 1173.5; HRMS: calcd for C38H38N2O4+H+, 587.29043; found (ESI-FTMS, [M+H]1+), 587.29016.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-ethynylbiphenyl-4-yl)ethyl]-L-α-glutamine

MS (ESI) m/z 529.2; HRMS: calcd for C34H30N2O4+H+, 531.22783; found (ESI-FTMS, [M+H]1+), 531.22669.


Example 10G
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-2-ylphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-2-ylphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 564.3; HRMS: calcd for C35H37N3O4+H+, 564.28568; found (ESI-FTMS, [M+H]1+), 564.28472.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-2-ylphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 508.2.


Example 10H
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-4-ylphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-4-ylphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 564.2; HRMS: calcd for C35H37N3O4+H+, 564.28568; found (ESI-FTMS, [M+H]1+), 564.28648.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-pyridin-4-ylphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 508.1; MS (ESI) m/z 275.1; HRMS: calcd for C31H29N3O4+H+, 508.22308; found (ESI-FTMS, [M+H]1+), 508.22403.


Example 10I
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-pyridin-2-ylphenyl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-pyridin-2-ylphenyl)ethyl]-L-α-glutaminate

MS (ESI) m/z 564.3; HRMS: calcd for C35H37N3O4+H+, 564.28568; found (ESI-FTMS, [M+H]1+), 564.28531.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(3-pyridin-2-ylphenyl)ethyl]-L-α-glutamine

MS (ESI) m/z 508.1; HRMS: calcd for C31H29N3O4+H+, 508.22308; found (ESI-FTMS, [M+H]1+), 508.22432.


Example 10J
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-3-yl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-3-yl)ethyl]-L-α-glutaminate
Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-formylbiphenyl-3-yl)ethyl]-L-α-glutamine

MS (ESI) m/z 535.2; MS (ESI) m/z 1069.3; HRMS: calcd for C33H30N2O5+H+, 535.22275; found (ESI-FTMS, [M+H]1+), 535.22147.


Example 10K
N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-3-yl]ethyl}-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-3-yl]ethyl}-L-α-glutaminate

HRMS: calcd for C37H37F3N2O4+H+, 631.27782; found (ESI-FTMS, [M+H]1+), 631.27639.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-{2-[4′-(trifluoromethyl)biphenyl-3-yl]ethyl}-L-α-glutamine

MS (ESI) m/z 575.2; HRMS: calcd for C33H29F3N2O4+H+, 575.21522; found (ESI-FTMS, [M+H]1+), 575.21382.


Example 10L
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-3-yl)ethyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-3-yl)ethyl]-L-α-glutaminate

HRMS: calcd for C37H40N2O5+H+, 593.30100; found (ESI-FTMS, [M+H]1+), 593.29993.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4′-methoxybiphenyl-3-yl)ethyl]-L-α-glutamine

MS (ESI) m/z 537.2; HRMS: calcd for C33H32N2O5+H+, 537.23840; found (ESI-FTMS, [M+H]1+), 537.23745.


Example 10M
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-vinylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-vinylbenzyl)-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate was used as the starting material, MS (ESI) m/z 499.2; MS (ESI) m/z 997.4; HRMS: calcd for C31H34N2O4+H+, 499.25913; found (ESI-FTMS, [M+H]1+), 499.25906.


Step B: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-vinylbenzyl)-L-α-glutamine

MS (ESI) m/z 443.2; MS (ESI) m/z 885.3; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19728.


Example 10N
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-thien-2-ylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-thien-2-ylbenzyl)-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate was used as the starting material. MS (ESI) m/z 555.2; MS (ESI) m/z 1109.3; HRMS: calcd for C33H34N2O4S+H+, 555.23120; found (ESI-FTMS, [M+H]1+), 555.23173.


Step B: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-thien-2-ylbenzyl)-L-α-glutamine

MS (ESI) m/z 499.1; HRMS: calcd for C29H26N2O4S+H+, 499.16860; found (ESI-FTMS, [M+H]1+), 499.16976.


Example 10O
N2-(biphenyl-4-ylcarbonyl)-N1-{[4′-(trifluoromethyl)biphenyl-3-yl]methyl}-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-{[4′-(trifluoromethyl)biphenyl-3-yl]methyl}-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate was used as the starting material, MS (ESI) m/z 617.2; MS (ESI) m/z 1233.3; HRMS: calcd for C36H35F3N2O4+H+, 617.26217; found (ESI-FTMS, [M+H]1+), 617.26334.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-{[4′-(trifluoromethyl)biphenyl-3-yl]methyl}-L-α-glutamine

MS (ESI) m/z 559.1; MS (ESI) m/z 1119.1; HRMS: calcd for C32H27F3N2O4+H+, 561.19957; found (ESI-FTMS, [M+H]1+), 561.2001.


Example 10P
N2-(biphenyl-4-ylcarbonyl)-N1-[(4′-ethynylbiphenyl-3-yl)methyl]-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-[(4′-ethynylbiphenyl-3-yl)methyl]-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate was used as the starting material, MS (ESI) m/z 573.2; MS (ESI) m/z 1145.4; HRMS: calcd for C37H36N2O4+H+, 573.27478; found (ESI-FTMS, [M+H]1+), 573.27518.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-[(4′-ethynylbiphenyl-3-yl)methyl]-L-α-glutamine

MS (ESI) m/z 517.2; MS (ESI) m/z 1033.3; HRMS: calcd for C33H28N2O4+H+, 517.21218; found (ESI-FTMS, [M+H]1+), 517.2125.


Example 10Q
N2-(biphenyl-4-ylcarbonyl)-N1-(3-pyridin-2-ylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-(biphenyl-4-ylcarbonyl)-N1-(3-pyridin-2-ylbenzyl)-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3-iodobenzyl)-L-α-glutaminate was used as the starting material, MS (ESI) m/z 550.2; HRMS: calcd for C34H35N3O4+H+, 550.27003; found (ESI-FTMS, [M+H]1+), 550.27066.


Step B: N2-(biphenyl-4-ylcarbonyl)-N1-(3-pyridin-2-ylbenzyl)-L-α-glutamine

MS (ESI) m/z 494.1; HRMS: calcd for C30H27N3O4+H+, 494.20743; found (ESI-FTMS, [M+H]1+), 494.20803.


Example 10R
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-vinylbenzyl)-L-α-glutamine
Step A: tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-vinylbenzyl)-L-α-glutaminate

Tert-butyl N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-iodobenzyl)-L-α-glutaminate was used as the starting material, MS (ESI) m/z 499.2; MS (ESI) m/z 997.3; HRMS: calcd for C31H34N2O4+H+, 499.25913; found (ESI-FTMS, [M+H]1+), 499.25883.


Step B: N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-vinylbenzyl)-L-α-glutamine

MS (ESI) m/z 443.1; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19697.







Example 11
N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate

A solution of 4-Amino-4-benzylcarbamoyl-butyric acid tert-butyl ester (6.39 g, 21.9 mmol, 1 equiv.) in DMF (18 mL) was added to 4-Bromobenzoic acid (4.39 g, 21.9 mmol, 1 equiv.) and PyBOP (14.7 g, 28.2 mmol, 1.2 equiv.) and the solution was stirred at room temperature under nitrogen. DIEA (4.58 mL, 26.3 mmol, 1.2 equiv.) was then added dropwise and the solution was stirred for 2 hrs. The reaction was complete as determined by TLC. The solution was diluted with EtOAc (250 mL) and washed consecutively with H2O, 10% HCl, satd. NaHCO3, brine, and dried over Na2SO4. After the solvent was evaporated, the crude residue was dissolved in ether and washed with aq. LiBr (2×250 mL) and dried over Na2SO4. A light tan solid was obtained, 8.94 g of tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate in 86% yield.


MS (ESI) m/z 475.1; MS (ESI) m/z 949.2; HRMS: calcd for C23H27BrN2O4+Na+, 497.10464; found (ESI_FTMS, [M+Na]1+), 497.10542.


Step B: tert-butyl N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutaminate

Tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate (200 mg, 0.421 mmol) was dissolved in toluene (4 mL) under nitrogen. Copper iodide (16.0 mg, 0.0841 mmol), triphenylarsine (26.0 mg, 0.0841 mmol), and Pd(Cl)2(PPh3)2 (59 mg, 0.0841 mmol) were then added as solids. The reaction mixture was then fitted with a condenser and heated to reflux (115° C.), for 10 minutes. 2-(Tributylstannyl)-thiophene (267 mL, 0.841 mmol) was added and immediately the reaction went from orange to black. These are typical Stille reaction conditions. The reaction was monitored by TLC and was complete at 30 minutes. The reaction mixture was filtered through celite and the celite rinsed with MeOH (1 mL). Solvent was removed and the remaining yellow residue was dissolved in a minimal amount of CH2Cl2. Hexanes were added, precipitating a white solid that was filtered, washed with hexanes, and then dried at reduced pressure. 140 mg of tert-butyl N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutaminate, a white solid was obtained in 69% yield. MS (ESI) m/z 479.1; MS (ESI) m/z 957.3; HRMS: calcd for C27H30N2O4S+H+, 479.19990; found (ESI_FT, [M+H]1+), 479.19826.


Step C: N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutamine

Tert-butyl N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutaminate (135 mg, 0.282 mmol) was dissolved in CH2Cl2 (1.5 mL) under nitrogen. TFA was diluted in CH2Cl2 (2 mL), and then added to the reaction. The reaction was monitored by TLC and was complete at 2 hrs. The solvent was removed and the remaining yellow residue was dissolved in toluene, which was then removed (2×) leaving an orange solid. The orange solid was taken up in acetone, and hexanes added, precipitating a pale orange solid. The solid was filtered, washed with hexanes, and dried at reduced pressure. The pale orange solid was run on a SiO2 column, using 4% MeOH/CH2Cl2. The solvent was removed from the fractions yielding 79 mg of N1-benzyl-N2-(4-thien-2-ylbenzoyl)-L-α-glutamine, a white solid, at 66% yield. MS (ESI) m/z 421.1; MS (ESI) m/z 843.3; HRMS: calcd for C23H22N2O4S+H+, 423.13730; found (ESI_FTMS, [M+H]1+), 423.13742.


Example 12

The following compounds were prepared according to procedures similar to those described in Example 11.


Example 12A
N1-benzyl-N2-[4-(2-furyl)benzoyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[4-(2-furyl)benzoyl]-L-α-glutaminate

MS (ESI) m/z 463.2; MS (ESI) m/z 925.3; HRMS: calcd for C27H30N2O5+H+, 463.22275; found (ESI_FT, [M+H]1+), 463.22159.


Step C: N1-benzyl-N2-[4-(2-furyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 407.1; MS (ESI) m/z 813.2; HRMS: calcd for C23H22N2O5+H+, 407.16015; found (ESI_FTMS, [M+H]1+), 407.16031.


Example 12B
N1-benzyl-N2-[(4′-ethynyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(4′-ethynyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 497.3; MS (ESI) m/z 993.5; HRMS: calcd for C31H32N2O4+H+, 497.24348; found (ESI_FTMS, [M+H]1+), 497.24306.


Step C: N1-benzyl-N2-[(4′-ethynyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 439.2; MS (ESI) m/z 879.4; HRMS: calcd for C27H24N2O4+H+, 441.18088; found (ESI_FT, [M+H]1+), 441.18056.


Example 12C
N1-benzyl-N2-(4-pyridin-3-ylbenzoyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-(4-pyridin-3-ylbenzoyl)-L-α-glutaminate

MS (ESI) m/z 474.2; HRMS: calcd for C28H31N3O4+H+, 474.23873; found (ESI_FT, [M+H]1+), 474.238741.


Step C: N1-benzyl-N2-(4-pyridin-3-ylbenzoyl)-L-α-glutamine

MS (ESI) m/z 418.1; HRMS: calcd for C24H23N3O4+H+, 418.17613; found (ESI_FT, [M+H]1+), 418.17555.


Example 12D
N1-benzyl-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutaminate

MS (ESI) m/z 480.2; MS (ESI) m/z 959.2; HRMS: calcd for C26H29N3O4S+H+, 480.19515; found (ESI_FT, [M+H]1+), 480.19659.


Step C: N1-benzyl-N2-[4-(1,3-thiazol-2-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 422.2; MS (ESI) m/z 845.3; HRMS: calcd for C22H21N3O4S+H+, 424.13255; found (ESI_FT, [M+H]1+), 424.13204.


Example 12E
N1-benzyl-N2-(4-pyridin-2-ylbenzoyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-(4-pyridin-2-ylbenzoyl)-L-α-glutaminate

MS (ESI) m/z 474.2; HRMS: calcd for C28H31N3O4+H+, 474.23873; found (ESI_FT, [M+H]1+), 474.2401.


Step C: N1-benzyl-N2-(4-pyridin-2-ylbenzoyl)-L-α-glutamine

MS (ESI) m/z 418.1; HRMS: calcd for C24H23N3O4+H+, 418.17613; found (ESI_FT, [M+H]1+), 418.17517.


Example 12F
N-benzyl-N2-[4-(2-naphthyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 467.2; MS (ESI) m/z 933.4; HRMS: calcd for C29H26N2O4+H+, 467.19653; found (ESI-FTMS, [M+H]1+), 467.19661.


Example 12G
N-benzyl-N2-[4-(phenylethynyl)benzoyl]-L-α-glutamine

MS (ESI) m/z 441.2; MS (ESI) m/z 881.4.


Example 12H
N-benzyl-N2-{4-[(9-hydroxy-9H-fluoren-9-yl)ethynyl]benzoyl}-L-α-glutamine

MS (ESI) m/z 543.1; MS (ESI) m/z 1087.2.


Example 12I
N-benzyl-N2-{4-[(1E)-3-oxo-3-phenylprop-1-en-1-yl]benzoyl}-L-α-glutamine

MS (ESI) m/z 471.2; MS (ESI) m/z 941.4.


Example 12J
N2-{4-[(3-aminophenyl)ethynyl]benzoyl}-N-benzyl-L-α-glutamine

MS (ESI) m/z 456.1; MS (ESI) m/z 249.1.


Example 12K
N-benzyl-N2-[4-(3-hydroxy-3,3-diphenylprop-1-yn-1-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 545.1; MS (ESI) m/z 1091.2; MS (ESI) m/z 659.1.


Example 12L
N-benzyl-N2-{4-[(3-methoxyphenyl)ethynyl]benzoyl}-L-α-glutamine

MS (ESI) m/z 471.1; MS (ESI) m/z 941.2.


Example 13

The following compounds were prepared according to procedures similar to those described in Example 11, except a Suzuki reaction was used at step B in place of a Stille reaction. Hydrolysis of the ester to the final product followed the same procedure as previously described.


Example 13A
N1-benzyl-N2-[(3′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(3′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

Tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate (350 mg, 0.736 mmol) was dissolved in DME, under nitrogen in a sealed vial. 3,5-Dimethylboronic acid (110 mg, 0.736 mmol), and Pd(PPh3)4 (43 mg, 0.037 mmol) were added as solids, and the reaction heated to reflux for 10 minutes. At this point 2M K2CO3 (735 mL, 1.47 mmol) was added, the reaction vial was sealed, and the reaction heated to reflux and monitored by TLC. The reaction was complete at 2 hrs. The aqueous part of the reaction was removed via pipette. The reaction solution was then filtered through celite and the celite rinsed with MeOH. The solvent was removed, leaving a brown solid. The product was purified using a SiO2 column and 33% EtOAc/Hex as the solvent. The product was obtained as a white solid, yielding 222 mg at 60% yield. MS (ESI) m/z 501.3; MS (ESI) m/z 1001.6; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FT, [M+H]1+), 501.27332.


Step C: N1-benzyl-N2-[(3′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI_FT) m/z 445.21151; MS (ESI_FT) m/z 445.21219; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FT, [M+H]1+), 445.21151.


Example 13B
N1-benzyl-N2-[(2′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(2′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 501.3; MS (ESI) m/z 1001.6; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FT, [M+H]1+), 501.27441.


Step C: N1-benzyl-N2-[(2′,5′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 445.3; MS (ESI) m/z 889.5; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FT, [M+H]1+), 445.21172.


Example 13C
N1-benzyl-N2-[(4′-ethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(4′-ethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 501.3; MS (ESI) m/z 1001.6; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FT, [M+H]1+), 501.27326.


Step C: N1-benzyl-N2-[(4′-ethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI_FT) m/z 445.2115; MS (ESI_FT) m/z 445.21219; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FT, [M+H]1+), 445.2115.


Example 13D
N1-benzyl-N2-[(3′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(3′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 517.3; MS (ESI) m/z 1033.6; HRMS: calcd for C31H36N2O5+H+, 517.26970; found (ESI_FT, [M+H]1+), 517.26837.


Step C: N1-benzyl-N2-[(3′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI_FT) m/z 461.20568; MS (ESI_FT) m/z 461.2071; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FT, [M+H]1+), 461.20568.


Example 13E
N1-benzyl-N2-[(2′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(2′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 517.3; MS (ESI) m/z 1033.5; HRMS: calcd for C31H36N2O5+H+, 517.26970; found (ESI_FT, [M+H]1+), 517.26833.


Step C: N1-benzyl-N2-[(2′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI_FT) m/z 461.20572; MS (ESI_FT) m/z 461.2071; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FT, [M+H]1+), 461.20572.


Example 13F
N1-benzyl-N2-[(2′,6′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(2′,6′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI_FT) m/z 501.27323; MS (ESI_FT) m/z 501.27479; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI_FT, [M+H]1+), 501.27323.


Step C: N1-benzyl-N2-[(2′,6′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI_FT) m/z 445.21145; MS (ESI_FT) m/z 445.21219; HRMS: calcd for C27H28N2O4+H+, 445.21218; found (ESI_FT, [M+H]1+), 445.21145.


Example 13G
N1-benzyl-N2-[(4′-vinyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(4′-vinyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 499.3; MS (ESI) m/z 997.5; HRMS: calcd for C31H34N2O4+H+, 499.25913; found (ESI_FT, [M+H]1+), 499.25855.


Step C: N1-benzyl-N2-[(4′-vinyl-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 443.2; MS (ESI) m/z 885.4; HRMS: calcd for C27H26N2O4+H+, 443.19653; found (ESI-FTMS, [M+H]1+), 443.19675.













Example 14
N1-benzyl-N2-[(4′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-[(4′-hydroxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

Tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate (3.00 g, 6.31 mmol) was dissolved in DME (40 mL), under nitrogen in a 75 mL bomb flask. 4-Hydroxyphenylboronic acid (871 mg, 6.31 mmol), and Pd(PPh3)4 (729 mg, 0.631 mmol) were added as solids, and the reaction heated to reflux for 30 minutes. At this point K2CO3 (1.74 g, 12.6 mmol) was dissolved in H2O (8 mL), and added to the reaction. The bomb flask was sealed and the reaction was heated to reflux, stirred overnight, and monitored by TLC. The reaction was complete after stirring overnight. The reaction mixture was filtered through celite and the celite rinsed with MeOH. The solvent was removed and the remaining solid was dissolved in EtOAc (200 mL) and then washed with 200 mL of 10% HCl (2×) and brine. The organic layer (top) was then dried (Na2SO4), filtered and concentrated, leaving a dark red oily solid. The product was purified using a SiO2 column, and 40% Ace/Hex as the solvent. It would not dissolve in this solvent so it was dissolved in Acetone/MeOH (1:1) and dry mounted on the column. The product was obtained as the third spot of the column and as a yellow solid. Yield 1.9 g of tert-butyl N1-benzyl-N2-[(4′-hydroxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate at 62% yield. MS (ESI) m/z 487.4; MS (ESI) m/z 533.5; MS (ESI) m/z 975.8; HRMS: calcd for C29H32N2O5+H+, 489.23840; found (ESI_FTMS, [M+H]1+), 489.23862.


Step B: tert-butyl N1-benzyl-N2-[(4′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

Tert-butyl N1-benzyl-N2-[(4′-hydroxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate (300 mg, 0.614 mmol) was dissolved in CH3CN (7 mL) in a sealed 20 mL vial. K2CO3 was added as a solid, the vial sealed and heated to 80° C. for 1 hour. Iodoethane (54 mL, 0.675 mmol) was added, the vial sealed and stirred at 80° C. overnight. The reaction was monitored by TLC and was complete after stirring overnight. The solvent was removed via nitrogen blower, and the remaining solid dissolved in CH2Cl2 (7 mL) and washed with 10% HCl (7 mL). The aqueous layer was decanted with a pipette, and the solvent was removed via nitrogen blower. The remaining solid was dissolved in DMSO (2 mL) and purified on a Gilson HPLC. Yield: 123 mg of tert-butyl N1-benzyl-N2-[(4′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate, a white solid at 39% yield. MS (ESI) m/z 517.2; MS (ESI) m/z 1033.4; HRMS: calcd for C31H36N2O5+H+, 517.26970; found (ESI_FTMS, [M+H]1+), 517.26801.


Step C: N1-benzyl-N2-[(4′-ethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

The procedure used was similar to that described in Example 13. MS (ESI) m/z 461.2; MS (ESI) m/z 921.4; HRMS: calcd for C27H28N2O5+H+, 461.20710; found (ESI_FTMS, [M+H]1+), 461.20542.


Example 15

The following compounds were prepared according to procedures similar to those described in Example 14.


Example 15A
N1-benzyl-N2-[(4′-propoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(4′-propoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 531.3; MS (ESI) m/z 1061.4; HRMS: calcd for C32H38N2O5+H+, 531.28535; found (ESI_FTMS, [M+H]1+), 531.2844.


Step C: N1-benzyl-N2-[(4′-propoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 475.2; MS (ESI) m/z 949.4; HRMS: calcd for C28H30N2O5+H+, 475.22275; found (ESI_FTMS, [M+H]1+), 475.22333.


Example 15B
N1-benzyl-N2-[(4′-butoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[(4′-butoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 545.3; MS (ESI) m/z 1089.5; HRMS: calcd for C33H40N2O5+H+, 545.30100; found (ESI_FTMS, [M+H]1+), 545.30048.


Step C: N1-benzyl-N2-[(4′-butoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 489.3; MS (ESI) m/z 977.4; HRMS: calcd for C29H32N2O5+H+, 489.23840; found (ESI_FTMS, [M+H]1+), 489.23871.


Example 15C
N1-benzyl-N2-{[4′-(cyclobutylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-{[4′-(cyclobutylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutaminate

MS (ESI) m/z 557.3; MS (ESI) m/z 1113.4; HRMS: calcd for C34H40N2O5+H+, 557.30100; found (ESI_FTMS, [M+H]1+), 557.30064.


Step C: N1-benzyl-N2-{[4′-(cyclobutylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 501.3; MS (ESI) m/z 1001.5; HRMS: calcd for C30H32N2O5+H+, 501.23840; found (ESI_FTMS, [M+H]1+), 501.23937.


Example 15D
N1-benzyl-N2-{[4′-(cyclohexylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-{[4′-(cyclohexylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutaminate

MS (ESI) m/z 585.3; MS (ESI) m/z 1169.5; HRMS: calcd for C36H44N2O5+H+, 585.33230; found (ESI_FTMS, [M+H]1+), 585.33275.


Step C: N1-benzyl-N2-{[4′-(cyclohexylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 529.3; MS (ESI) m/z 1057.5; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FTMS, [M+H]1+), 529.27022.


Example 15E
N2-{[4′-(allyloxy)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine
Step B: tert-butyl N2-{[4′-(allyloxy)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutaminate

MS (ESI) m/z 529.2; MS (ESI) m/z 1057.4; HRMS: calcd for C32H36N2O5+H+, 529.26970; found (ESI_FTMS, [M+H]1+), 529.26804.


Step C: N2-{[4′-(allyloxy)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine
Example 15F
N1-benzyl-N2-({4′-[(3-methoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-({4′-[(3-methoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutaminate

MS (ESI) m/z 609.3; MS (ESI) m/z 1217.5; HRMS: calcd for C37H40N2O6+H+, 609.29591; found (ESI-FTMS, [M+H]1+), 609.29669.


Step C: N1-benzyl-N2-({4′-[(3-methoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 553.2; MS (ESI) m/z 1105.3; HRMS: calcd for C33H32N2O6+H+, 553.23331; found (ESI-FTMS, [M+H]1+), 553.23235.


Example 15G
N1-benzyl-N2-({4′-[(3,5-dimethoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-({4′-[(3,5-dimethoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutaminate

MS (ESI) m/z 639.3; MS (ESI) m/z 1277.4; HRMS: calcd for C38H42N2O7+H+, 639.30648; found (ESI-FTMS, [M+H]1+), 639.30698.


Step C: N1-benzyl-N2-({4′-[(3,5-dimethoxybenzyl)oxy]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 583.2; MS (ESI) m/z 1165.3; HRMS: calcd for C34H34N2O7+H+, 583.24388; found (ESI-FTMS, [M+H]1+), 583.2433.


Example 15H
N1-benzyl-N2-{[4′-(2-naphthylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-{[4′-(2-naphthylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutaminate

MS (ESI) m/z 629.3; HRMS: calcd for C40H40N2O5+H+, 629.30100; found (ESI-FTMS, [M+H]1+), 629.30277.


Step C: N1-benzyl-N2-{[4′-(2-naphthylmethoxy)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 571.3; HRMS: calcd for C36H32N2O5+H+, 573.23840; found (ESI-FTMS, [M+H]1+), 573.23779.


Example 15I
N-benzyl-N2-({4′-[(3-fluorobenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 541.1; MS (ESI) m/z 1081.2.


Example 15J
N-benzyl-N2-{[4′-(benzyloxy)biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 523.1; MS (ESI) m/z 1045.2.


The following compounds were prepared according to Scheme 10, Route A:


Example 15K
N2-{[3′-(benzyloxy)biphenyl-4-yl]carbonyl}-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 613.1; MS (ESI) m/z 1225.1; HRMS: calcd for C35H36N2O8+H+, 613.25444; found (ESI-FTMS, [M+H]1+), 613.25451.


Example 15L
N2-({3′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 673.3; MS (ESI) m/z 1345.6; MS (ESI) m/z 690.4; HRMS: calcd for C37H40N2O10+H+, 673.27557; found (ESI-FTMS, [M+H]1+), 673.27431.


Example 15M
N2-({3′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 643.3; MS (ESI) m/z 1285.6; HRMS: calcd for C36H38N2O9+H+, 643.26501; found (ESI-FTMS, [M+H]1+), 643.26574.


Example 15N
N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 643.3; MS (ESI) m/z 1285.6; HRMS: calcd for C37H39FN2O7+H+, 643.28141; found (ESI-FTMS, [M+H]1+), 643.28187.


The following compounds were prepared according to Scheme 10, Route B:


Example 15O
N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine
Step A: tert-butyl N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutaminate

tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate (1.00 g, 1.93 mmol) was dissolved in DME (10 mL) under nitrogen and placed in a microwave vial. 4-methylphenyl boronic acid (526 mg, 3.87.mmol), and Pd(PPh3)4 (334 mg, 0.290 mmol) were added as solids prior to the addition of K2CO3 (800 mg, 5.79 mmol) dissolved in H2O (2.3 mL). The vial was sealed and heated to 80° C. in a microwave for 20 minutes. The solvent was removed and the remaining yellow oil was dissolved in diethyl ether (125 mL). The organics were washed consecutively with 10% HCl (125 mL), water (125 mL) and brine (125 mL). The organic layer was dried with MgSO4, filtered through celite and the solvent was removed. The sticky solid was purified using on a 40 g Combi flash column using an increasing gradient of EtOAc/Hex as the solvent. The product was obtained as a light brown solid. Yield 792 mg of tert-butyl N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutaminate at 78% yield. MS (ESI) m/z 529.3; HRMS: calcd for C33H40N2O4+H+, 529.30608; found (ESI-FTMS, [M+H]1+), 529.30594.


Step B: tert-butyl N2-{[4′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate

tert-butyl N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutaminate (3.5 g, 6.62 mmol) was dissolved in CCl4 (50 mL) along with N-Bromosuccinimide (2.36 g, 13.2 mmol) and benzoyl peroxide (321 mg, 1.32 mmol) in a 100 mL flask equipped with a reflux condenser. The reaction mixture stirred overnight at 70° C. The solvent was removed and the residue was purified using a SiO2 column and 20% EtOAc/Hex as the solvent giving a yellow solid. Yield 1.05 g tert-butyl N2-{[4′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate at 57% yield. MS (ESI) m/z 607.1; HRMS: calcd for C33H39BrN2O4+H+, 607.21660; found (ESI-FTMS, [M+H]1+), 607.21591.


Step C: tert-butyl N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate

tert-butyl N2-{[4′-(bromomethyl)biphenyl-4-yl]carbonyl}-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate (253 mg, 0.41 mmol) 3,5-bis(trifluoromethyl)phenol (63 μL, 42 mmol) and K2CO3 (287 mg, 2.08 mmol) were all combined in a 10 mL vial. Acetonitrile (4 mL) was added dissolving some of the solids upon heating to 80° C. The reaction was monitored using TLC and MS. The reaction was stirred overnight (18 h). The reaction was filtered using a fritted funnel and the solvent was removed. The filtrate was collected and the solvent was removed. The residue was purified using a SiO2 column and 20% EtOAc/Hex as the solvent giving a white solid. Yield 242 mg of tert-butyl N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate at 77% yield. MS (ESI) m/z 757.4; HRMS: calcd for C41H42F6N2O5+H+, 757.30707; found (ESI-FTMS, [M+H]1+), 757.30532.


Step D: N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

tert-butyl N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutaminate (215 mg, 0.284 mmol) was dissolved in CH2Cl2 (3 mL) and stirred in a 10 mL flask. Trifluoroacetic acid (1.09 mL, 14 mmol) was added and the solution was stirred for 2 hours. The solvent was removed and the dried under vacuum. The resulting residue was purified using preparative HPLC (ACN/H2O, 0.02% TFA). The main peak fractions were collected and after removal of solvent gave the product as a white solid. Yield of 104 mg of N2-[(4′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine was obtained in 52% yield. MS (ESI) m/z 699.3; HRMS: calcd for C37H34F6N2O5+H+, 701.24447; found (ESI-FTMS, [M+H]1+), 701.24429.


The following compounds were prepared according to the procedure described above for example 15O:


Example 15P
N2-({4′-[(1,3-benzodioxol-5-yloxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 609.3; MS (ESI) m/z 1217.6; HRMS: calcd for C36H36N2O7+H+, 609.25953; found (ESI-FTMS, [M+H]1+), 609.26109.


Example 15Q
N2-({4′-[(3,5-dimethoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 595.4; HRMS: calcd for C35H36N2O7+H+, 597.25953; found (ESI-FTMS, [M+H]1+), 597.25901.


Example 15R
N2-({3′-[(3,5-dimethoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 595.2; HRMS: calcd for C35H36N2O7+H+, 597.25953; found (ESI-FTMS, [M+H]1+), 597.2593.


Example 15S
N2-[(3′-{[3,5-bis(trifluoromethyl)phenoxy]methyl}biphenyl-4-yl)carbonyl]-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 671.2; MS (ESI) m/z 1343.7; HRMS: calcd for C35H30F6N2O5+H+, 673.21317; found (ESI-FTMS, [M+H]1+), 673.21279.


Example 15T
N2-({3′-[(3-ethylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 563.3; HRMS: calcd for C35H36N2O5+H+, 565.26970; found (ESI-FTMS, [M+H]1+), 565.26999.


The following compounds were prepared according to Scheme 10, Route C:


Example 15U
N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-[(4′-hydroxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate

tert-butyl N1-benzyl-N2-(4-bromobenzoyl)-L-α-glutaminate (2.00 g, 4.21 mmol) was dissolved in DME (20 mL) under nitrogen and placed in a 75 mL flask. 4-Hydroxyphenyl boronic acid (638 mg, 4.63.mmol), and Pd(PPh3)4 (729 mg, 0.632 mmol) were added as solids prior to the addition of K2CO3 (1.75 g, 12.6 mmol) dissolved in H2O (5 mL). The flask was sealed and the reaction was heated to reflux, stirred for two days and monitored by TLC. The reaction mixture was filtered through celite and then rinsed with MeOH. The solvent was removed and the resulting residue was purified using a SiO2 column and 33% EtOAc/Hex as the solvent. The product was obtained as a white solid. Yield: 514 mg of tert-butyl N1-benzyl-N2-[(4′-hydroxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutaminate at 25% yield. MS (ESI) m/z 487.4; HRMS: calcd for C29H32N2O5+H+, 489.23840; found (ESI_FTMS, [M+H]1+), 489.23862.


Step B: tert-butyl N-benzyl-N2-({4′-[(3-bromobenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutaminate

3,3-bromobenzylbromide and K2CO3 were all combined in a 20 mL vial. CH3CN (6 mL) was added, dissolving some of the solids upon heating to 80° C. The reaction was monitored using TLC and MS. After 2 hours the reaction was complete. The resulting product quickly precipitated upon cooling of the CH3CN. The reaction was filtered using a fritted funnel and the filtrate was collected and redissolved in warm CH3CN. The solution was allowed to crystallize overnight in the freezer. The white solid was collected and washed with cold CH3CN. Yield: 357 mg of tert-butyl N-benzyl-N2-({4′-[(3-bromobenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutaminate at 57% yield. MS (ESI) m/z 657.2.


Step C: tert-butyl N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutaminate

tert-butyl N-benzyl-N2-({4′-[(3-bromobenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutaminate (250 mg, 0.38 mmol) was dissolved in DME (5 mL) under nitrogen in a 75 mL bomb flask. Phenylboronic acid (51 mg, 0.418 mmol), and Pd(PPh3)4 (66 mg, 0.057 mmol) were added as solids prior to the addition of K2CO3 (158 mg, 1.14 mmol) dissolved in H2O (1.5 mL). The bomb flask was sealed and the reaction was heated to reflux, stirred two days and monitored by TLC. The reaction mixture was filtered through celite and the celite rinsed with MeOH. The solvent was removed and the resulting residue was purified using preparative HPLC. The product was obtained as a pale orange solid. Yield: 154 mg of tert-butyl N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutaminate at 60% yield. MS (ESI) m/z 655.5; HRMS: calcd for C42H42N2O5+H+, 655.31665; found (ESI-FTMS, [M+H]1+), 655.31636.


Step D: N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine

tert-butyl N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutaminate (130 mg, 0.199 mmol) was dissolved in CH2Cl2 (3 mL) and stirred in a 10 mL flask. Trifluoroacetic acid (765 uL, 10 mmol) was added and the solution was stirred for 2 hours. The solvent was removed and the dried under vacuum. The orange residue was dissolved in minimum acetone and precipitated with hexanes. Yield of 109 mg of N-benzyl-N2-{[4′-(biphenyl-3-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine was obtained in 91% yield. MS (ESI) m/z 599.4; HRMS: calcd for C38H34N2O5+H+, 599.25405; found (ESI-FTMS, [M+H]1+), 599.25511.


Example 15V
N-benzyl-N2-({4′-[(3′-methoxybiphenyl-3-yl)methoxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 627.2; HRMS: calcd for C39H36N2O6+H+, 629.26461; found (ESI-FTMS, [M+H]1+), 629.26493.


Example 15W
N-benzyl-N2-{[4′-(biphenyl-2-ylmethoxy)biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 597.3; HRMS: calcd for C38H34N2O5+H+, 599.25405; found (ESI-FTMS, [M+H]1+), 599.25523.


Example 15X
N-benzyl-N2-({4′-[(3′-methoxybiphenyl-2-yl)methoxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 627.3; HRMS: calcd for C39H36N2O6+H+, 629.26461; found (ESI-FTMS, [M+H]1+), 629.26484.


Example 15Y
N2-({4′-[(3-tert-butylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

Tolyl-biphenylglutamate (1 g, 1.89 mmol) was dissolved in CCl4 (20 mL), in a 40 mL sealed vial. N-Bromosuccinimide (NBS) (673 mg, 3.78 mmol) and Benzoyl peroxide (92 mg, 0.378 mmol) were added. The reaction was heated to 70° C., and stirred overnight. The reaction was cooled and the solvent removed. The remaining sticky orange solid was purified by silica column chromatography using 20% EtOAc/Hexanes. 400 mg of Bromo-tolyl-biphenylglutamate was obtained, as a pale yellow solid at 36% yield.


3-t-Butylphenol (56 mg, 0.374 mmol) was dissolved in CH3CN in a 20 ml sealed vial and K2CO3 (258 mg, 1.87 mmol) was added. The reaction was heated to 80° C. and stirred for 30 minutes. The Bromo-tolyl-biphenylglutamate (250 mg, 0.411 mmol) was added and the reaction stirred at 80° C. overnight. The reaction was filtered, the solvent was removed and the remainder of the reaction was purified by silica column chromatography to give the title compound as a white solid was obtained in 87% yield.


MS (ESI) m/z 621.2; MS (ESI) m/z 1241.4; HRMS: calcd for C39H44N2O5+H+, 621.33230; found (ESI-FTMS, [M+H]1+), 621.33234.


The following compounds were prepared according to procedures similar to those described in Example 15Y:


Example 15Z
N2-({4′-[(3,5-di-tert-butylphenoxy)methyl]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 675.3; MS (ESI) m/z 1351.6; HRMS: calcd for C43H52N2O5+H+, 677.39490; found (ESI-FTMS, [M+H]1+), 677.39504.


Example 15AA
N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-ethylphenoxy)methyl]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 593.2; MS (ESI) m/z 1185.4; HRMS: calcd for C37H40N2O5+H+, 593.30100; found (ESI-FTMS, [M+H]1+), 593.30054.


Example 15BB
N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-methoxyphenoxy)methyl]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 595.3; MS (ESI) m/z 1189.6; HRMS: calcd for C36H38N2O6+H+, 595.28026; found (ESI-FTMS, [M+H]1+), 595.28031.


Example 16
N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

The title compound was prepared according to procedures similar to those described in Examples 7 and 11. MS (ESI) m/z 551.2; MS (ESI) m/z 1101.4; MS (ESI) m/z 573.2; HRMS: calcd for C30H34N2O8+H+, 551.23879; found (ESI-FTMS, [M+H]1+), 551.23735.


The following compounds were prepared according to procedures similar to those described in Example 16.


Example 17A
N2-[(2′-ethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 551.2; MS (ESI) m/z 1101.4; MS (ESI) m/z 573.2; HRMS: calcd for C30H34N2O8+H+, 551.23879; found (ESI-FTMS, [M+H]1+), 551.23739.


Example 17B
N2-[(4′-methoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 537.2; MS (ESI) m/z 1073.4; MS (ESI) m/z 559.2; HRMS: calcd for C29H32N2O8+H+, 537.22314; found (ESI-FTMS, [M+H]1+), 537.22188.


Example 17C
N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

HRMS: calcd for C29H32N2O8+H+, 537.22314; found (ESI-FTMS, [M+H]1+), 537.22188.


Example 17D
4′-{[((1S)-3-carboxy-1-{[(3,4,5-trimethoxybenzyl)amino]carbonyl}propyl)amino]carbonyl}biphenyl-3-carboxylic acid

MS (ESI) m/z 551.1; MS (ESI) m/z 1101.3; MS (ESI) m/z 573.1; HRMS: calcd for C29H30N2O9+H+, 551.20241; found (ESI-FTMS, [M+H]1+), 551.2008.


Example 17E
N2-[(4′-ethylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 535.2; MS (ESI) m/z 1069.4; HRMS: calcd for C30H34N2O7+H+, 535.24388; found (ESI-FTMS, [M+H]1+), 535.2425.


Example 17F
N2-[(3′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 567.2; MS (ESI) m/z 1133.5; MS (ESI) m/z 589.2; HRMS: calcd for C30H34N2O9+H+, 567.23371; found (ESI-FTMS, [M+H]1+), 567.23327.


Example 17G
N2-[(2′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 567.2; MS (ESI) m/z 1133.5; HRMS: calcd for C30H34N2O9+H+, 567.23371; found (ESI-FTMS, [M+H]1+), 567.23397.


Example 17H
N-(3,4,5-trimethoxybenzyl)-N2-[(3′,4′,5′-trimethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 597.2; MS (ESI) m/z 1193.4; HRMS: calcd for C31H36N2O10+H+, 597.24427; found (ESI-FTMS, [M+H]1+), 597.24385.


Example 17I
N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 489.2; MS (ESI) m/z 977.4; MS (ESI) m/z 511.2; HRMS: calcd for C29H32N2O5+H+, 489.23840; found (ESI-FTMS, [M+H]1+), 489.2373.


Example 17J
N2-[(3′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 519.2; MS (ESI) m/z 1037.5; HRMS: calcd for C30H34N2O6+H+, 519.24896; found (ESI-FTMS, [M+H]1+), 519.248971.


Example 17K
N-(1,1-dimethyl-2-phenylethyl)-N2-[(2′-ethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.5; HRMS: calcd for C30H34N2O5+H+, 503.25405; found (ESI-FTMS, [M+H]1+), 503.25421.


Example 17L
N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 491.2; MS (ESI) m/z 981.5; HRMS: calcd for C29H31FN2O4+H+, 491.23406; found (ESI-FTMS, [M+H]1+), 491.23402.


Example 17M
4-[(4′-Methoxy-3′-methyl-biphenyl-4-carbonyl)-amino]-4-(3,4,5-trimethoxy-benzylcarbamoyl)-butyric acid

MS (ESI) m/z 551.2; MS (ESI) m/z 1101.5; MS (ESI) m/z 1123.5.


Example 17N
4-(4-Benzo[1,3]dioxol-5-yl-benzoylamino)-4-(3,4,5-trimethoxy-benzylcarbamoyl)-butyric acid

MS (ESI) m/z 551.2; MS (ESI) m/z 1101.4.


Example 17O
N-(1-adamantylmethyl)-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 491.4; MS (ESI) m/z 981.7; HRMS: calcd for C29H34N2O5+H+, 491.25405; found (ESI-FTMS, [M+H]1+), 491.25465.


Example 17P
N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.4; MS (ESI) m/z 525.2; HRMS: calcd for C29H30N2O6+H+, 503.21766; found (ESI-FTMS, [M+H]1+), 503.21771.


Example 17Q
N2-[(2′,4′-dimethoxybiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 519.2; MS (ESI) m/z 1037.5; MS (ESI) m/z 541.2; HRMS: calcd for C30H34N2O6+H+, 519.24896; found (ESI-FTMS, [M+H]1+), 519.24917.


Example 17R
N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 539.3; MS (ESI) m/z 1077.6; MS (ESI) m/z 561.3; HRMS: calcd for C29H31FN2O7+H+, 539.21881; found (ESI-FTMS, [M+H]1+), 539.2182.


Example 17S
N2-[(4′-sec-butylbiphenyl-4-yl)carbonyl]-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 515.4; MS (ESI) m/z 1029.9; HRMS: calcd for C32H38N2O4+H+, 515.29043; found (ESI-FTMS, [M+H]1+), 515.29203.


Example 17T
N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-isopropylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 501.4; MS (ESI) m/z 1001.8; HRMS: calcd for C31H36N2O4+H+, 501.27478; found (ESI-FTMS, [M+H]1+), 501.27468.


Example 17U
N-(1,1-dimethyl-2-phenylethyl)-N2-(1,1′:4′,1″-terphenyl-4-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 535.4; MS (ESI) m/z 1069.8; HRMS: calcd for C34H34N2O4+H+, 535.25913; found (ESI-FTMS, [M+H]1+), 535.26044.


Example 17V
N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 521.3; MS (ESI) m/z 1043.7; HRMS: calcd for C28H30N2O8+H+, 523.20749; found (ESI-FTMS, [M+H]1+), 523.20878.


Example 17W
N-(1-adamantylmethyl)-N2-({3′-[(dimethylamino)carbonyl]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 546.4; MS (ESI) m/z 1091.9; HRMS: calcd for C32H39N3O5+H+, 546.29625; found (ESI-FTMS, [M+H]1+), 546.29678.


Example 17X
N-(1-adamantylmethyl)-N2-[(3′,5′-dimethylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 503.4; MS (ESI) m/z 1005.8; HRMS: calcd for C31H38N2O4+H+, 503.29043; found (ESI-FTMS, [M+H]1+), 503.29107.


Example 17Y
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 491.4; MS (ESI) m/z 981.7; MS (ESI) m/z 513.3; HRMS: calcd for C29H31FN2O4+H+, 491.23406; found (ESI-FTMS, [M+H]1+), 491.23228.


Example 17Z
N2-[(3′-isopropylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 549.4; MS (ESI) m/z 1097.9; HRMS: calcd for C31H36N2O7+H+, 549.25953; found (ESI-FTMS, [M+H]1+), 549.26013.


Example 17AA
N2-[(4′-isopropylbiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 549.4; MS (ESI) m/z 1097.9; HRMS: calcd for C31H36N2O7+H+, 549.25953; found (ESI-FTMS, [M+H]1+), 549.26066.


Example 17BB
N-(1-adamantylmethyl)-N2-[4-(1-benzofuran-5-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 515.4; MS (ESI) m/z 1029.9; HRMS: calcd for C31H34N2O5+H+, 515.25405; found (ESI-FTMS, [M+H]1+), 515.25447.


Example 17CC
N-(1-adamantylmethyl)-N2-[4-(1H-indol-5-yl)benzoyl]-L-α-glutamine

MS (ESI) m/z 514.4; MS (ESI) m/z 1027.7; HRMS: calcd for C31H35N3O4+H+, 514.27003; found (ESI-FTMS, [M+H]1+), 514.27038.


Example 17DD
N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 521.4; MS (ESI) m/z 1041.7; HRMS: calcd for C30H33FN2O5+H+, 521.24463; found (ESI-FTMS, [M+H]1+), 521.24248.


Example 17EE
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-methoxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 507.3; MS (ESI) m/z 1013.7; HRMS: calcd for C29H31FN2O5+H+, 507.22898; found (ESI-FTMS, [M+H]1+), 507.22717.


Example 17FF
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-isopropylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 519.4; MS (ESI) m/z 1037.7; HRMS: calcd for C31H35FN2O4+H+, 519.26536; found (ESI-FTMS, [M+H]1+), 519.26474.


Example 17GG
N2-[(3′-fluoro-4′-methylbiphenyl-4-yl)carbonyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

MS (ESI) m/z 509.3; MS (ESI) m/z 1017.7; HRMS: calcd for C29H30F2N2O4+H+, 509.22464; found (ESI-FTMS, [M+H]1+), 509.22537.


Example 17HH
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(4′-isobutylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 533.4; MS (ESI) m/z 1065.8; HRMS: calcd for C32H37FN2O4+H+, 533.28101; found (ESI-FTMS, [M+H]1+), 533.28214.


Example 17II
N2-(1,1′:4′,1″-terphenyl-4-ylcarbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 581.2; MS (ESI) m/z 1163.4; HRMS: calcd for C34H34N2O7+H+, 583.24388; found (ESI-FTMS, [M+H]1+), 583.24414.


Example 17JJ
N-benzyl-N2-{[3′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 445.1; MS (ESI) m/z 891.2.


Example 17KK
N-(3,4,5-trimethoxybenzyl)-N2-[(2′,4′,6′-trimethylbiphenyl-4-yl)carbonyl]-L-α-glutamine

HRMS: calcd for C31H36N2O7+H+, 549.25953; found (ESI-FTMS, [M+H]1+), 549.25817.


Example 17LL
N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 475.2; MS (ESI) m/z 949.4; HRMS: calcd for C28H30N2O5+H+, 475.22275; found (ESI-FTMS, [M+H]1+), 475.22358.


Example 17MM
N-(1,1-dimethyl-2-phenylethyl)-N2-[(3′-ethoxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 503.2; MS (ESI) m/z 1005.3; MS (ESI) m/z 525.1; HRMS: calcd for C30H34N2O5+H+, 503.25405; found (ESI-FTMS, [M+H]1+), 503.25252.


Example 17NN
N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI) m/z 475.1; MS (ESI) m/z 949.2; HRMS: calcd for C27H26N2O6+H+, 475.18636; found (ESI-FTMS, [M+H]1+), 475.1872.


Example 17OO
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(4′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 493.2; MS (ESI) m/z 985.4; HRMS: calcd for C28H29FN2O5+H+, 493.21333; found (ESI-FTMS, [M+H]1+), 493.21327.


Example 17PP
N2-[4-(1,3-benzodioxol-5-yl)benzoyl]-N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-L-α-glutamine

(1): MS (ESI) m/z 521.1; MS (ESI) m/z 1041.3; HRMS: calcd for C29H29FN2O6+H+, 521.20824; found (ESI-FTMS, [M+H]1+), 521.20872; (2): MS (ESI) m/z 521.2;


MS (ESI) m/z 1041.3.


Example 17QQ
N-benzyl-N2-{4-[(4-hydroxycyclohexyl)ethynyl]benzoyl}-L-α-glutamine

MS (ESI) m/z 463.1; MS (ESI) m/z 925.3.


Example 17RR
N2-[(3′-hydroxybiphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

HRMS: calcd for C28H30N2O8+H+, 523.20749; found (ESI-FTMS, [M+H]1+), 523.20809.


Example 17SS
N-(1,1-dimethyl-2-phenylethyl)-N2-{[4′-(hydroxymethyl)biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 487.1; MS (ESI) m/z 975.2; HRMS: calcd for C29H32N2O5+H+, 489.23840; found (ESI-FTMS, [M+H]1+), 489.2389.


Example 17TT
N-(1,1-dimethyl-2-phenylethyl)-N2-[(4′-methylbiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 473.1; MS (ESI) m/z 945.3; HRMS: calcd for C29H32N2O4+H+, 473.24348; found (ESI-FTMS, [M+H]1+), 473.24409.


Example 17UU
N-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N2-[(3′-hydroxybiphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 493.2; MS (ESI) m/z 985.4.


Example 17VV
N1-benzyl-N2-(4-pyridin-4-ylbenzoyl)-L-α-glutamine

MS (ESI) m/z 418.2; HRMS: calcd for C24H23N3O4+H+, 418.17613; found (ESI_FT, [M+H]1+), 418.17461.


Example 17WW
N1-benzyl-N2-(1,1′-biphenyl-3-ylcarbonyl)-L-α-glutamine

MS (ESI) m/z 417.1; MS (ESI) m/z 833.1; HRMS: calcd for C25H24N2O4+H+, 417.18088; found (ESI-FTMS, [M+H]1+), 417.18049.


Example 17XX
N1-benzyl-N2-(3-thien-2-ylbenzoyl)-L-α-glutamine

MS (ESI) m/z 423.1; MS (ESI) m/z 845; HRMS: calcd for C23H22N2O4S+H+, 423.13730; found (ESI-FTMS, [M+H]1+), 423.1383.


Example 17YY
N1-benzyl-N2-[(3-chloro-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 451.1; MS (ESI) m/z 901; HRMS: calcd for C25H23ClN2O4+H+, 451.14191; found (ESI-FTMS, [M+H]1+), 451.1417.


Example 17ZZ
N1-benzyl-N2-[(3-fluoro-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

HRMS: calcd for C25H23FN2O4+H+, 435.17146; found (ESI-FTMS, [M+H]1+), 435.17091.


Example 17AAA
N1-benzyl-N2-[(2,6-dimethoxy-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamine

HRMS: calcd for C27H28N2O6+H+, 477.20201; found (ESI-FTMS, [M+H]1+), 477.20098.


Example 17BBB
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(4-methoxy-1,1′-biphenyl-3-yl)-L-α-glutamine

MS (ESI) m/z 509.2; MS (ESI) m/z 1017.2; HRMS: calcd for C31H28N2O5+H+, 509.20710; found (ESI-FTMS, [M+H]1+), 509.20875.


Example 18
N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 673.4; MS (ESI) m/z 1345.9; HRMS: calcd for C37H40N2O10+H+, 673.27557; found (ESI-FTMS, [M+H]1+), 673.27498.


Example 19

The following compounds were prepared according to procedures similar to those described in Example 18.


Example 19A
N-(1-adamantylmethyl)-N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 611.5; MS (ESI) m/z 1222; HRMS: calcd for C37H42N2O6+H+, 611.31156; found (ESI-FTMS, [M+H]1+), 611.31164.


Example 19B
N-(1-adamantylmethyl)-N2-(4′-{[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 641.5; MS (ESI) m/z 1282; HRMS: calcd for C38H44N2O7+H+, 641.32213; found (ESI-FTMS, [M+H]1+), 641.32171.


Example 19C
N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 625.5; MS (ESI) m/z 1249.9; HRMS: calcd for C37H40N2O7+H+, 625.29083; found (ESI-FTMS, [M+H]1+), 625.2906.


Example 19D
N2-({4′-[(3,5-dimethylbenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 641.5; MS (ESI) m/z 1282.


Example 19E
N2-[(4′-{[3,5-bis(trifluoromethyl)benzyl]oxy}biphenyl-4-yl)carbonyl]-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 749.5; MS (ESI) m/z 771.5; HRMS: calcd for C37H34F6N2O8+H+, 749.22921; found (ESI-FTMS, [M+H]1+), 749.2297.


Example 19F
N-(1,1-dimethyl-2-phenylethyl)-N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-L-α-glutamine

MS (ESI) m/z 595.3; MS (ESI) m/z 1189.5; HRMS: calcd for C36H38N2O6+H+, 595.28026; found (ESI-FTMS, [M+H]1+), 595.28185.


Example 19G
N2-({4′-[(3,5-dimethoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(6-hydroxyhexyl)-L-α-glutamine

MS (ESI) m/z 591.2; HRMS: calcd for C33H40N2O8+H+, 593.28574; found (ESI-FTMS, [M+H]1+), 593.28726.


Example 19H
N2-({4′-[(3-methoxybenzyl)oxy]biphenyl-4-yl}carbonyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI-FTMS) m/z 643.26499; MS (ESI-FTMS) m/z 643.26501; HRMS: calcd for C36H38N2O9+H+, 643.26501; found (ESI-FTMS, [M+H]1+), 643.26499.







Example 20
N1-benzyl-N2-(5-phenyl-2-furoyl)-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-(5-bromo-2-furoyl)-L-α-glutaminate

Tert-butyl N1-benzyl-L-α-glutaminate (2.33 g, 7.98 mmol) was dissolved in DMF (7 mL) under nitrogen. 5-Bromofuroic acid (1.52 g, 7.98 mmol) was added, followed by addition of PyBOP (5.00 g, 9.6 mmol). Finally, DIEA (1.67 mL, 9.6 mmol) was added dropwise over 5 minutes via addition funnel. The reaction was monitored by TLC and stirred overnight. The solution was diluted with EtOAc (250 mL), washed consecutively with H2O, 10% HCl, saturated NaHCO3, brine, and then dried over Na2SO4. After solvent was evaporated, the crude residue was dissolved in ether and washed with aq. LiBr (2×250 mL) and dried over Na2SO4. Ether was removed, leaving an oil which was further purified on a SiO2 column using 33%-40% EtOAc/Hex as the solvent. The product was the third spot off the column and a sticky orange solid. Yield 1.60 g of tert-butyl N1-benzyl-N2-(5-bromo-2-furoyl)-L-α-glutaminate at 43% yield.


Step B: tert-butyl N1-benzyl-N2-(5-phenyl-2-furoyl)-L-α-glutaminate

HRMS: calcd for C27H30N2O5+Na+, 485.20469; found (ESI FTMS, [M+Na]1+), 485.20466.


Step C: N1-benzyl-N2-(5-phenyl-2-furoyl)-L-α-glutamine

HRMS: calcd for C23H22N2O5+H+, 407.16015; found (ESI_FTMS, [M+H]1+), 407.15841.


Example 21

The following compounds were prepared according to procedures similar to those described in Example 20.


Example 21A
N1-benzyl-N2-[5-(3-ethoxyphenyl)-2-furoyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[5-(3-ethoxyphenyl)-2-furoyl]-L-α-glutaminate

HRMS: calcd for C29H34N2O6+H+, 507.24896; found (ESI_FTMS, [M+H]1+), 507.24894.


Step C: N1-benzyl-N2-[5-(3-ethoxyphenyl)-2-furoyl]-L-α-glutamine

HRMS: calcd for C25H26N2O6+H+, 451.18636; found (ESI_FTMS, [M+H]1+), 451.18545.


Example 21B
N1-benzyl-N2-[5-(3,5-dimethylphenyl)-2-furoyl]-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-[5-(3,5-dimethylphenyl)-2-furoyl]-L-α-glutaminate

HRMS: calcd for C29H34N2O5+H+, 491.25405; found (ESI_FTMS, [M+H]1+), 491.2538.


Step C: N1-benzyl-N2-[5-(3,5-dimethylphenyl)-2-furoyl]-L-α-glutamine

HRMS: calcd for C25H26N2O5+H+, 435.19145; found (ESI_FTMS, [M+H]1+), 435.19034.


Example 21C
N1-benzyl-N2-(2,2′-bifuran-5-ylcarbonyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-(2,2′-bifuran-5-ylcarbonyl)-L-α-glutaminate

HRMS: calcd for C25H28N2O6+H+, 453.20201; found (ESI_FTMS, [M+H]1+), 453.20173.


Step C: N1-benzyl-N2-(2,2′-bifuran-5-ylcarbonyl)-L-α-glutamine

HRMS: calcd for C21H20N2O6+H+, 397.13941; found (ESI_FTMS, [M+H]1+), 397.13777.


Example 21D
N1-benzyl-N2-(5-thien-2-yl-2-furoyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2 (5-thien-2-yl-2-furoyl)-L-α-glutaminate

HRMS: calcd for C25H28N2O5S+H+, 469.17917; found (ESI_FTMS, [M+H]1+), 469.1789.


Step C: N1-benzyl-N2-(5-thien-2-yl-2-furoyl)-L-α-glutamine

HRMS: calcd for C21H20N2O5S+H+, 413.11657; found (ESI_FTMS, [M+H]1+), 413.11511.







Example 22

The following compounds were prepared according to procedures similar to those described in Example 20.


Example 22A
N1-benzyl-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine
Step A: tert-butyl N1-benzyl-N2-[(6-bromopyridin-3-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 476.1; MS (ESI) m/z 951.1; HRMS: calcd for C22H26BrN3O4+H+, 476.11794; found (ESI-FTMS, [M+H]1+), 476.11935.


Step B: tert-butyl N1-benzyl-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 474.2; HRMS: calcd for C28H31N3O4+H+, 474.23873; found (ESI-FTMS, [M+H]1+), 474.23861.


Step C: N1-benzyl-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 418.1; HRMS: calcd for C24H23N3O4+H+, 418.17613; found (ESI-FTMS, [M+H]1+), 418.17674.


Example 22B
N1-(3-methoxybenzyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine
Step A: tert-butyl N2-[(6-bromopyridin-3-yl)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 506.1; MS (ESI) m/z 1011.1; HRMS: calcd for C23H28BrN3O5+H+, 506.12851; found (ESI-FTMS, [M+H]1+), 506.12839.


Step B: tert-butyl N1-(3-methoxybenzyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutaminate

MS (ESI) m/z 504.2; HRMS: calcd for C29H33N3O5+H+, 504.24930; found (ESI-FTMS, [M+H]1+), 504.24835.


Step C: N1-(3-methoxybenzyl)-N2-[(6-phenylpyridin-3-yl)carbonyl]-L-α-glutamine

MS (ESI) m/z 448.1; HRMS: calcd for C25H25N3O5+H+, 448.18670; found (ESI-FTMS, [M+H]1+), 448.18686.


Example 22C
N2-[(2,2′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutamine
Step B: tert-butyl N2-[(2,2′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutaminate

MS (ESI) m/z 531.3; MS (ESI) m/z 1061.4; HRMS: calcd for C32H38N2O5+H+, 531.28535; found (ESI_FTMS, [M+H]1+), 531.2855.


Step C: N2-[(2,2′-dimethyl-1,1′-biphenyl-4-yl)carbonyl]-N1-(3-methoxybenzyl)-L-α-glutamine

HRMS: calcd for C28H30N2O5+H+, 475.22275; found (ESI_FTMS, [M+H]1+), 475.22271.


Example 22D
N2-(4-bromobenzoyl)-N-(3,4,5-trimethoxybenzyl)-L-α-glutamine

MS (ESI) m/z 507; MS (ESI) m/z 1015.1; HRMS: calcd for C22H25BrN2O7+H+, 509.09179; found (ESI-FTMS, [M+H]1+), 509.09078.


Example 22E
N2-(4-bromobenzoyl)-N-(1,1-dimethyl-2-phenylethyl)-L-α-glutamine

MS (ESI) m/z 461.1; MS (ESI) m/z 921.2; HRMS: calcd for C22H25BrN2O4+H+, 461.10705; found (ESI-FTMS, [M+H]1+), 461.10805.


Example 22F
N-(1-adamantylmethyl)-N2-(4-bromobenzoyl)-L-α-glutamine

MS (ESI) m/z 477.3; MS (ESI) m/z 953.5; HRMS: calcd for C23H29BrN2O4+H+, 477.13835; found (ESI-FTMS, [M+H]1+), 477.13801.


Example 22G
N2-(4-bromobenzoyl)-N-(3-methylbenzyl)-L-α-glutamine

MS (ESI-FTMS) m/z 433.07562; MS (ESI-FTMS) m/z 433.07575; HRMS: calcd for C20H21BrN2O4+H+, 433.07575; found (ESI-FTMS, [M+H]1+), 433.07562.







Example 23
N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine
Step A: tert-butyl N2-[(4′-amino-1,1′-biphenyl-4-yl)carbonyl]-N1-benzyl-L-α-glutaminate

4-Benzylcarbamoyl-4-(4-bromo-benzoylamino)-butyric acid tert-butyl ester (3.0 g, 6.31 mmol, 1 equiv.) was dissolved in DME (60 mL) was added to 4-(4,4,5,5)-tetramethyl-1,3,2-dioxoborolan-2-yl)aniline (1.38 g, 6.31 mmol, 1 equiv.) and Pd(PPh3)4 (728 mg, 0.063 mmol, 0.1 equiv.). The mixture was stirred for 30 minutes prior to the addition of aq. K2CO3 (1.74 g, 12.6 mmol, 2 equiv.) in 12 mL H2O. The mixture was capped in a sealed vessel and stirred overnight at 80° C. The reaction was complete as determined by TLC. The mixture was filtered over celite, the solvent was removed, and the resulting tan solid was diluted with EtOAc (200 mL) and washed consecutively with H2O, 10% HCl, and brine, and then dried over Na2SO4. The solid was purified by column chromatography (silica gel, 41% Acetone/Hexanes) to give 1.89 g of tert-butyl N2-[(4′-amino-1,1′-biphenyl-4-yl)carbonyl]-N1-benzyl-L-α-glutaminate in 61% yield. MS (ESI) m/z 488.2; HRMS: calcd for C29H33N3O4+H+, 488.25438; found (ESI_FTMS, [M+H]1+), 488.25433.


Step B: isopropyl N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutaminate

4-[(4′-Amino-biphenyl-4-carbonyl)-amino]-4-benzylcarbamoyl-butyric acid tert-butyl ester (300 mg, 0.62 mmol, 1 equiv.) was dissolved in CH2Cl2 (20 mL) and cooled to 0° C. followed by the addition of proton sponge (266 mg, 1.24 mmol, 2 equiv.). The mixture was stirred 30 minutes prior to the addition of acetyl chloride (48 μL, 0.68 mmol, 1.1 equiv.) The mixture was stirred at 0° C. for 1.5 hrs and allowed to warm to room temperature. Reaction was complete as determined by TLC. The mixture was washed consecutively with H2O, NaHCO3 satd., and brine, and then dried over Na2SO4. The tan solid was purified by preparative HPLC to give 85 mg of isopropyl N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutaminate in 26% yield.


MS (ESI) m/z 557.2; MS (ESI) m/z 279.1.


Step C: N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine

4-[(4′-Acetylamino-biphenyl-4-carbonyl)-amino]-4-benzylcarbamoyl-butyric acid tert-butyl ester (75 mg) was dissolved in dichloroethane (4 mL) and added to TFA (2 mL). The mixture was stirred at room temperature for 3 hrs. Reaction was complete as determined by TLC. The solvent was removed and the resulting solid was purified by preparative HPLC to give N2-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine in 54% yield (37 mg). MS (ESI) m/z 474.2; MS (ESI) m/z 947.3; HRMS: calcd for C27H27N3O5+H+, 474.20235; found (ESI_FTMS, [M+H]1+), 474.20274.


Example 24

The following compounds were prepared according to procedures similar to those described in Example 23.


Example 24A
N1-benzyl-N2-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutaminate

MS (ESI) m/z 582.3; MS (ESI) m/z 1163.5; HRMS: calcd for C34H35N3O6+H+, 582.25986; found (ESI_FTMS, [M+H]1+), 582.26058.


Step C: N1-benzyl-N2-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamine

MS (ESI) m/z 526.2; MS (ESI) m/z 1051.3; HRMS: calcd for C30H27N3O6+H+, 526.19726; found (ESI FTMS, [M+H]1+), 526.19773.


Example 24B
N1-benzyl-N2-({4′-[(4-fluorobenzoyl)amino]1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine
Step B: tert-butyl N1-benzyl-N2-({4′-[(4-fluorobenzoyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutaminate
Step C: N1-benzyl-N2-({4′-[(4-fluorobenzoyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamine
Example 24C
N2-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-N1-benzyl-L-α-glutamine

HRMS: calcd for C32H29N3O5—H+, 534.20345; found (ESI-FTMS, [M−H]1−), 534.2024.







Example 25
4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid
Step A: 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester

4-Diphenyl carboxylic acid (3.57 g, 18 mmol) and EDCI (4.28 g, 22.3 mmol, 1.2 equiv.) were dissolved in DMF (40 mL). The mixture was stirred at room temperature for 0.5 hrs. H-Glu(OMe)OtBu (5.02 g, 19.8 mmol, 1.1 equiv.) was added, followed by the addition of Et3N (6.77 mL, 48.6 mmol, 2.7 equiv.) and DMAP (330 mg, 2.7 mmol, 15%). The mixture was stirred overnight under N2. The reaction mixture was then diluted with EtOAc and washed with H2O and brine. The organic layer was dried over MgSO4, concentrated, and purified by column chromatography (20% EtOAc/Hexane) to afford 3.2 g of 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester in 44.7% yield. MS (ESI) m/z 396.


Step B: 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester

2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 1-tert-butyl ester 5-methyl ester (1.5 g, 3.8 mmol) was dissolved in dichloroethane (26 mL) and the mixture was added to TFA (13 mL). The mixture was stirred at room temperature for 4 hrs. TLC indicated the reaction was complete. Solvent was evaporated by rotovap. The solid obtained was washed with EtOAc and water. After drying, 2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester was obtained in 87% yield (1.128 g). MS (ESI) m/z 342.


Step C: 4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid methyl ester

2-[(Biphenyl-4-carbonyl)-amino]-pentanedioic acid 5-methyl ester (1.128 g, 3.30 mmol, 1.0 equiv.) was dissolved in THF (35 mL). The mixture was cooled to −20° C., followed by the addition of Et3N (343 mg, 3.3 mmol, 1.0 equiv.) and ethyl chloroformate (358 mg, 3.3 mmol, 1.0 equiv.). After stirring for 20 minutes, NaBH4 (375 mg, 9.9 mmol, 3 equiv.) was added and the temperature was raised to −10° C. After stirring for 5 min, MeOH (30 mL) was introduced and the mixture was further stirred for 20 min at this temperature and then at 0° C. for 20 min. 1N HCl was then added slowly to quench the reaction. Solvent was evaporated and the residue partitioned between EtOAc and H2O. The organic layer was washed with brine, dried over MgSO4, and removed solvent by rotavap. The crude mixture was then purified by column chromatography (silica gel, 3% MeOH/CH2Cl2) to afford 333 mg of 4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid methyl ester in 31% yield. MS (ESI) m/z 328.


Step D: 4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid

A solution of 4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid methyl ester (333 mg, 1.06 mmol) in THF (10 mL) and MeOH (1.27 mL) was added to 1N NaOH (1.27 mL, 1.27 mmol, 1.2 equiv) at room temperature. The mixture was stirred for 2 hours and TLC indicated the reaction was complete. Solvent was removed and the residue was dissolved in water. After adjusting the pH to 4 with 1N HCl, solid precipitated from the solution. The solid was then collected by filtration, washed with water, and dried under vacuum overnight to afford 240 mg of 4-[(Biphenyl-4-carbonyl)-amino]-5-hydroxy-pentanoic acid in 76.7% yield. MS (ESI) m/z 314.







Example 26
N-({4′-[(Phenylacetyl)amino]1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamic acid
Step A: 2-(4-Bromo-benzoylamino)-pentanedioic acid di-tert-butyl ester

A solution of 4-Bromobenzoic acid (4 g, 19.9 mmol, 1 equiv) in DMF (140 mL) was added to EDCI (5.72 g, 29.8 mmol, 1.5 equiv.) and the mixture was stirred at room temperature for 25 min. Et3N (6.9 mL, 49.7 mmol, 2.5 equiv) was then added, followed by addition of DMAP (486 mg, 4.0 mmol, 20%). The reaction mixture was allowed to stir at room temperature overnight. Reaction was complete as determined by TLC. DMF was removed by rotavap and the residue partitioned between EtOAc and brine solution. The organic layer was separated, dried over MgSO4 and concentrated. The crude mixture was purified by column chromatography (silica gel, 20% EtOAc/Hexane) to give 4.2 g of 2-(4-Bromo-benzoylamino)-pentanedioic acid di-tert-butyl ester in 47.7% yield. MS (ESI) m/z 441.


Step B: 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester

4-(4,4,5,5-Tetramethyl-[1,3,2]dioxaborolan-2-yl)-phenylamine (1.34 g, 6.104 mmol, 1 equiv.) and 2-(4-Bromo-benzoylamino)-pentanedioic acid di-tert-butyl ester (2.7 g, 6.104 mmol, 1 equiv.) were dissolved in DME (120 mL). A solution of K2CO3 (1.68 g, 12.21 mmol, 2 equiv.) in 30 mL of water was injected into the mixture, followed by the addition of Pd(PPh3)4 (352 mg, 0.31 mmol, 5%). The mixture was heated to reflux overnight. Reaction was complete as determined by TLC. Regular work-up and column chromatography (silica gel, 30-40% EtOAc/n-Hexane) afforded 2.3 g of 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester in 83% yield. MS (ESI) m/z 453.


Step C: Phenylacetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester

A solution of phenylacetic acid (89 mg, 0.65 mmol, 1 equiv) in DMF (10 mL) was added to EDCI (188 mg, 0.98 mmol, 1.5 equiv.). After stirring for 20 min, 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (297 mg, 0.65 mmol, 1 equiv.) was added, followed by the addition of Et3N (0.23 mL, 1.64 mmol, 2.5 equiv.) and DMAP (16 mg, 0.13 mmol, 20%). The reaction mixture was allowed to stir at room temperature overnight. Reaction was complete as determined by TLC. DMF was removed and the residue was dissolved in EtOAc. The organic layer was washed with H2O and brine, and then dried over MgSO4. After solvent was evaporated, the crude residue was purified by HPLC to give 45 mg of 2-[(4′-Phenylacetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester in 12% yield. MS (ESI) m/z 573.


Step D: N-({4′-[(phenylacetyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid

2-[(4′-Phenylacetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (45 mg) was dissolved in dichloroethane (3 mL) and added to TFA (1 mL). The mixture was stirred at room temperature for 3 hrs. Reaction was complete as determined by TLC. Solvent was removed and the resulting solid was dried under vacuum overnight. N-({4′ [(phenylacetyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid was obtained in 84.2% yield (30.3 mg). MS (ESI) m/z 461.


Example 27
N-[(4′-{[(3-Methyl-1-benzofuran-2-yl)carbonyl]-amino}-1,1′-biphenyl-4-yl)carbonyl]-L-α-glutamic acid






The title compound was prepared according to procedures similar to those described in Example 26.


Step C: 2-({4′-[(3-Methyl-benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester

EDCI coupling between 3-Methylbenzofuran 2 carboxylic acid (139 mg, 0.79 mmol) and 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (358 mg, 0.79 mmol, 1 equiv.) was carried out according to the procedure in step C of Example 5 to give 2-({4′-[(3-Methyl-benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester in 12.4% yield (60 mg). MS (ESI) m/z 611.


Step D: N-[(4′-{[(3-Methyl-1-benzofuran-2-yl)carbonyl]-amino}-1,1′-biphenyl-4-yl)carbonyl]-L-glutamic acid

Hydrolysis of 2-({4′-[(3-Methyl-benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester (60 mg) was carried out according to procedure in step D of Example 5 to give N-[(4′-{[(3-Methyl-1-benzofuran-2-yl)carbonyl]-amino}-1,1′-biphenyl-4-yl)carbonyl]-L-glutamic acid in 59% yield (28.9 mg). MS (ESI) m/z 499.


Example 28
N-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamic acid






Step A: 2-({4′-[(Furan-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester

To a solution of 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (350 mg, 0.77 mmol) and 2-Furoic acid (87 mg, 0.77 mmol, 1 equiv.) in DMF (6 mL) was added BOP reagent (409 mg, 0.92 mmol, 1.2 equiv.) and Hunig base (0.16 mL, 0.92 mmol, 1.2 equiv.) under N2. The mixture was stirred at room temperature overnight. Reaction was complete was determined by LC-MS. The reaction mixture was then poured onto cold water. Solid precipitated from the mixture was collected by filtration. The solid was then dissolved in EtOAc, washed with 1N HCl, saturated NaHCO3, and brine, and then dried over MgSO4. After removing the solvent, the solid was dried under vacuum overnight to provide 2-({4′-[(Furan-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester in 87% yield (369 mg). MS (ESI) m/z 549.


Step B: N-{[4′-(2-furoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid

2-({4′-[(Furan-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester (369 mg, 0.67 mmol) was dissolved in dichloroethane (10 mL) and added to TFA (5 mL) at 0° C. The cooling bath was removed after the addition was complete. The mixture was stirred at room temperature for 3 hrs. and the reaction was complete as determined by TLC. After removing solvent, the residue was washed 3 times with EtOAc. The solid was then dried under vacuum to give 2-({4′-[(Furan-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid in 85.6% yield (251 mg). MS (ESI) m/z 437.


Example 29
N-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamic acid






The title compound was prepared according to procedures similar to those described in Example 28.


Step A: 2-[(4′-Acetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester

BOP coupling of 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (350 mg, 0.77 mmol) was carried out according to procedures in step A of Example 6B to give 344 mg of 2-[(4′-Acetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester in 89% yield. MS (ESI) m/z 497.


Step B: N-{[4′-(acetylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid

Hydrolysis of 2-[(4′-Acetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (344 mg, 0.69 mmol) was carried out according to procedures in step B for Example 6B to give 212 mg of 2-[(4′-Acetylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid in 79.7% yield. MS (ESI) m/z 383.


Example 30
N-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-α-glutamic acid






The title compound was prepared according to procedures similar to those described for Example 6B.


Step D: N-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid

Hydrolysis of 2-[(4′-Benzoylamino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (191 mg) was carried out according to procedures in step D of Example 6B to give N-{[4′-(benzoylamino)-1,1′-biphenyl-4-yl]carbonyl}-L-glutamic acid in 61.6% yield (93.7 mg). MS (ESI) m/z 445.


Example 31
N-({4′-[(1-benzofuran-2-ylcarbonyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-α-glutamic acid






The title compound was prepared according to the procedures similar to that described for Example 28.


Step A: 2-({4′-[(Benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester

BOP coupling of 2-[(4′-Amino-biphenyl-4-carbonyl)-amino]-pentanedioic acid di-tert-butyl ester (349 mg, 0.77 mmol) with 2-Benzofuran carboxylic acid (124 mg, 0.77 mmol, 1 equiv.) was carried out according to procedures in step A of Example 6B to give 185 mg of 2-({4′-[(Benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester in 40% yield. MS (ESI) m/z 597.


Step B: N-({4′-[(1-benzofuran-2-ylcarbonyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid

Hydrolysis of 2-({4′-[(Benzofuran-2-carbonyl)-amino]-biphenyl-4-carbonyl}-amino)-pentanedioic acid di-tert-butyl ester (185 mg, 0.31 mmol) was carried out according to procedures in step B of Example 6B to give N-({4′-[(1-benzofuran-2-ylcarbonyl)amino]-1,1′-biphenyl-4-yl}carbonyl)-L-glutamic acid in 82.5% yield (124 mg). MS (ESI) m/z 485.







Example 32
N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N5-hydroxy-L-α-glutamamide






To a solution of the starting carboxylic acid (0.476 g, 1 mmol) in DMF (4 mL) under nitrogen is added BOP reagent (0.442 g, 1 mmol), followed by addition of hydroxylamine hydrochloride (0.076 g, 1.1 mmol), and DIEA (0.38 mL, 2.2 mmol). The reaction mixture was stirred for 24 hrs at room temperature. The mixture was then added slowly to 50 mL of water. The resulting white solid was collected, washed several times with water and dried under vacuum to give 350 mg of N2-(biphenyl-4-ylcarbonyl)-N1-[2-(4-fluorophenyl)-1,1-dimethylethyl]-N5-hydroxy-L-glutamamide.


Example 33

The following compounds were prepared according to procedures similar to those described in Example 32.


Example 33A
N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-(3,4,5-trimethoxybenzyl)-L-α-glutamamide

MS (ESI) m/z 522.2; HRMS: calcd for C28H31N3O7+H+, 522.22348; found (ESI_FTMS, [M+H]1+), 522.22365.


Example 33B
N2-(1,1′-biphenyl-4-ylcarbonyl)-N1-(3,4-dimethylbenzyl)-N5-hydroxy-L-α-glutamamide

MS (ESI) m/z 460.2; MS (ESI) m/z 919.4; HRMS: calcd for C27H29N3O4+H+, 460.22308; found (ESI_FTMS, [M+H]1+), 460.22328.


Example 33C
N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-[2-(3-methoxyphenyl)ethyl]-L-α-glutamamide

MS (ESI) m/z 476.2; MS (ESI) m/z 951.4; HRMS: calcd for C27H29N3O5+H+, 476.21800; found (ESI_FTMS, [M+H]1+), 476.21792.


Example 33D
N2-(1,1′-biphenyl-4-ylcarbonyl)-N5-hydroxy-N1-[3-(trifluoromethoxy)benzyl]-L-α-glutamamide

MS (ESI) m/z 516.2; MS (ESI) m/z 1031.4; HRMS: calcd for C26H24F3N3O5+H+, 516.17408; found (ESI_FTMS, [M+H]1+), 516.17389.


Example 34
In Vitro Fluorescence Screen for Aggrecanase-1 Inhibitors
Materials and Methods

This assay is a preliminary screen of potential aggrecanase-1 inhibitors at 11 concentrations to determine their ability to inhibit cleavage of a fluorescent peptide (Abz-TEGEARGSVI-Dap(Dnp)-KK). A GeminiXS fluorimeter (Molecular Devices) was turned on and the temperature was set to 30° C., ˜30 min before setting up the assay. The following buffers were used: 50 mM HEPES, pH 7.5; 100 mM NaCl; 5 mM CaCl2; 0.1% CHAPS; and 5% glycerol. The source of enzyme in the assay was purified recombinant human Aggrecanase-1 (rAgg1), used at 5 μg/ml (final concentration in the assay). The substrate used in the assay was the peptide (AnaSpec, MWT=1645.8, stored at 4° C.). A stock solution of peptide was prepared at 2 mg/ml in 100% DMSO. The absorbance at 354 nm (ε=18172 M−1 cm−1) was measured to determine the exact concentration of the stock solution. The stock solution was then diluted to 62.5 μM in buffer. The unused 100% DMSO stock was stored at −80° C. The final concentration of the substrate in the assay was 25 μM. Inhibitor solutions were prepared in 100% DMSO with a 10× starting concentration, and serial dilutions were performed (in duplicate) across the dilution plate in 100% DMSO.


The assay was performed as follows: 96-well plates were set up so that the final column of wells was used for controls. The total reaction volume of each well was 100 μl. Each compound was assayed in duplicate. Buffer was added to the entire 96-well plate (30 μl/well). rAgg1 was diluted to 25 μg/ml buffer just prior to addition on the plate, and added to the wells at 20 μl/well. 10 μl/well of 10× inhibitors was added to the appropriate wells from the working plate, except for the control wells, to which are added 10 μl/well of a 10× reference control compound or 100% DMSO. The mixtures were incubated for 10-15 min at 30° C. 40 μl/well of 62.5 μM peptide substrate was then added to the appropriate wells.


Results

The reaction was monitored for 30-40 min at 30° C., with λex=340 nm and λ em=420 nm. The fluorescence was linear during this time and the slope of the line (Vmax/sec) represents the initial reaction rate,ν. The maximal rate of cleavage of substrate was determined in the absence of inhibitor. The percent inhibition of activity in the presence of inhibitor was calculated as follows: % inhibition=(1−ν(Rate, RFU/sec)/Maximal Rate(RFU/sec))*100. The IC50 was obtained by fitting the initial rate, ν, or % inhibition at each concentration of inhibitor to the following equation: y=(a−d)/(1+C/IC50)̂n)+d. The assay results are shown in Tables 1, 2, and 3 below.


This model describes a sigmoidal curve with an adjustable baseline, a. y is the % inhibition or initial rate of reaction, C is the concentration of inhibitor under test. A is the limiting response as C approaches zero. As C increases without bound, y tends toward its lower limit, d. y is halfway between the lower and upper asymptotes when C=IC50. n is the Hill coefficient. The sign of n is positive when the response increases with increasing dose and is negative when the response decreases with increasing dose (inhibition). (See, Knight, C. G. Methods Enzymol. 1995, 248, 18-34; Knight, C. G. et al., FEBS Lett. 1992, 296, 263-266; Jin, G. et al., Anal. Biochem. 2002, 302, 269-275).


Example 35
In Vitro Fluorescence Screen for Aggrecanase-2 Inhibitors
Materials and Methods

A continuous assay was used in which the substrate is a synthetic peptide containing a fluorescent group that is quenched by energy transfer. Cleavage of the peptide by the enzyme results in a large increase in fluorescence. The initial rates of this reaction were compared to the initial rates of reactions containing inhibitors in order to assess the potency of small molecules. The source of enzyme in the assay was purified recombinant human Aggrecanase-2 prepared at Wyeth Research (Biological Chemistry, Cambridge). More specifically, the form used is denoted as Ag2t-Phe628-Strep (MW=41,737). This form is truncated relative to the full-length enzyme and contains an affinity tag. Aliquots of this enzyme were stored at 80° C. in 25 mM Tris (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 10 μM ZnCl2, 10% glycerol. The substrate in the assay was a synthetic peptide that is designed after a portion of brevican, one of the naturally occurring substrates of Aggrecanase-2. This peptide contains the fluorescent group 2-aminobenzoyl (Abz) that is quenched by energy transfer to a 2,4-dinitrophenyl group (Dnp). The peptide (mass=1740) is >95% pure based on HPLC analysis and has the sequence: Abz TESESRGAIY-Dap(Dnp)-KK-NH2. Stock solutions of the substrate were prepared with MilliQ water and aliquots were stored at 80° C. The concentration of this substrate stock was spectrophotometrically determined using the extinction coefficient at 354 nm of 18,172 M−1 cm−1. The Vmax and Km for this enzyme/substrate reaction were determined to be insensitive to DMSO up to at least 10% (v/v). The assay buffer (pH 7.4) consisted of 50 mM Hepes, 100 mM NaCl, 5 mM CaCl2, 0.1% CHAPS, 5% glycerol.


Each well of the 96-well or 384-well plates contained a reaction consisting of assay buffer, purified Agg-2 (diluted with assay buffer), and varied concentrations of inhibitor (prepared by serial dilution in DMSO in 96-well polypropylene plates). The plates were then incubated at room temperature for 10 minutes. The enzymatic reactions were initiated by adding substrate to a final concentration of 25 μM and mixed by pipetting up and down. The initial rates of the cleavage reactions were determined at room temperature with a fluorescence plate reader immediately after substrate addition. The Agg-2 assays were run on a Tecan Safire with the following wavelength settings: excitation—316 nm, 12 nm bandwidth; emission—432 nm, 12 nm bandwidth.


Results

A plot of time vs. RFU, representing the progress curve of the reaction, was generated. The slope for the portion of the progress curve that is most linear was determined. This slope (RFU/min) is the initial rate of the reaction. Plots of the inhibitor concentration vs. the initial cleavage rate were fit to the following equation: y=Vmax*(1−(xn/(Kn+xn))), whereby x=inhibitor concentration, y=initial rate, Vmax=initial rate in the absence of inhibitor, n=slope factor, and K=IC50 for the inhibition curve. The assay results are shown in Tables 1, 2, and 3 below.


Example 36
In Vitro Fluorescence Assay of MMP-13 Activity
Materials and Methods

A continuous assay was used in which the substrate is a synthetic peptide containing a fluorescent group (7-methoxycoumarin; Mca), which is quenched by energy transfer to a 2,4-dinitrophenyl group. When the peptide was cleaved by MMP, a large increase in fluorescence was observed. The source of enzyme in the assay was the recombinant human catalytic domain of MMP-13 (165 amino acids, residues 104-268, 19 kDa) prepared at Wyeth-Research in Cambridge. The substrate used was the peptide Mca-PQGL-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-AR-OH. The assay buffer consisted of 50 mM Hepes (pH 7.4), 100 mM NaCl, 5 mM CaCl2, and 0.005% Brij-35. Each well of the 96-well plates contained a 200 μL reaction mixture consisting of assay buffer, purified MMP (final concentration of 0.5 nM, prepared by diluting with the assay buffer), and varied concentrations of inhibitor (prepared by serially diluting a given inhibitor in DMSO in 96-well polypropylene plate). The plates were then incubated at 30° C. for 15 minutes. The enzymatic reactions were initiated by adding the substrate to a final concentration of 20 μM, and mixing 10 times with a pipette. The final DMSO concentration in the assay was 6.0%. The initial rate of the cleavage reaction was determined at 30° C. temperature with a fluorescence plate reader (excitation filter of 330 nm and emission filter of 395 nm) immediately after substrate addition.


Results

Plots of the inhibitor concentration vs. the percent inhibition were fit to the following equation: y=(a−d)/[1+(x/c)b]+d, a general sigmoidal curve with Hill slope, a to d. x is the inhibitor concentration under test. y is the percent inhibition. a is the limiting response as x approaches zero. As x increases without bound, y tends toward its limit d. c is the inflection point (IC50) for the curve. That is, y is halfway between the lower and upper asymptotes when x=c. b is the slope factor or Hill coefficient. (See, Knight, C. G. et al., FEBS Lett. 1992, 296, 263-266). The assay results are shown in Tables 1, 2, and 3 below.


Example 37
In Vitro Fluorescence Assay of MMP-14 Activity
Materials and Materials

A continuous assay was used in which the substrate is a synthetic peptide containing a fluorescent group (7-methoxycoumarin; Mca), which is quenched by energy transfer to a 2,4-dinitrophenyl group. When the peptide was cleaved by MMP, a large increase in fluorescence was observed. The source of enzyme in the assay was the recombinant human catalytic domain of MMP-14 (177 amino acids corresponding to Tyr89-Gly265 of mature human enzyme; 20 kDa; Chemicon International, Inc. (catalog number CC1041)). The substrate used was the peptide Mca-PQGL-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-AR-OH. The assay buffer consisted of 50 mM Hepes (pH 7.4), 100 mM NaCl, 5 mM CaCl2, and 0.005% Brij-35. Each well of the 96-well plates contained a 200 μL reaction mixture consisting of assay buffer, MMP (final concentration of 25 ng/ml, prepared by diluting with the assay buffer), and varied concentrations of inhibitor (prepared by serially diluting a given inhibitor in DMSO in 96-well polypropylene plate). The plates were then incubated at 30° C. for 15 minutes. The enzymatic reactions were initiated by adding the substrate to a final concentration of 20 μM, and mixing 10 times with a pipette. The final DMSO concentration in the assay was 6.0%. The initial rate of the cleavage reaction was determined at 30° C. temperature with a fluorescence plate reader (excitation filter of 330 nm and emission filter of 395 nm) immediately after substrate addition.


Results

Plots of the inhibitor concentration vs. the percent inhibition were fit to the following equation: y=(a−d)/[1+(x/c)b]+d, a general sigmoidal curve with Hill slope, a to d. x is the inhibitor concentration under test. y is the percent inhibition. a is the limiting response as x approaches zero. As x increases without bound, y tends toward its limit d. c is the inflection point (IC50) for the curve. That is, y is halfway between the lower and upper asymptotes when x=c. b is the slope factor or Hill coefficient. (See, Knight, C. G. et al., FEBS Lett. 1992, 296, 263-266). The assay results are shown in Tables 1, 2, and 3 below.


Example 38
In Vitro Fluorescence Assay of MMP-2 Activity
Materials and Methods

A continuous assay was used in which the substrate is a synthetic peptide containing a fluorescent group (7-methoxycoumarin; Mca), which is quenched by energy transfer to a 2,4-dinitrophenyl group. When the peptide was cleaved by MMP, a large increase in fluorescence was observed. The source of enzyme in the assay was the recombinant human MMP-2 (66 kDa; Oncogene Research Products (catalog number PF023 from Calbiochem)). The substrate used was the peptide Mca-PQGL-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)-AR-OH. The assay buffer consisted of 50 mM Hepes (pH 7.4), 100 mM NaCl, 5 mM CaCl2, and 0.005% Brij-35. Each well of the 96-well plates contained a 200 μL reaction mixture consisting of assay buffer, MMP (final concentration of 25 ng/ml, prepared by diluting with the assay buffer), and varied concentrations of inhibitor (prepared by serially diluting a given inhibitor in DMSO in 96-well polypropylene plate). The plates were then incubated at 30° C. for 15 minutes. The enzymatic reactions were initiated by adding the substrate to a final concentration of 20 μM, and mixing 10 times with a pipette. The final DMSO concentration in the assay was 6.0%. The initial rate of the cleavage reaction was determined at 30° C. temperature with a fluorescence plate reader (excitation filter of 330 nm and emission filter of 395 nm) immediately after substrate addition.


Results

Plots of the inhibitor concentration vs. the percent inhibition were fit to the following equation: y=(a−d)/[1+(x/c)b]+d, a general sigmoidal curve with Hill slope, a to d. x is the inhibitor concentration under test. y is the percent inhibition. a is the limiting response as x approaches zero. As x increases without bound, y tends toward its limit d. c is the inflection point (IC50) for the curve. That is, y is halfway between the lower and upper asymptotes when x=c. b is the slope factor or Hill coefficient. (See, Knight, C. G. et al., FEBS Lett. 1992, 296, 263-266). The assay results are shown in Tables 1, 2, and 3 below.


Example 39
In Vitro Articular Cartilage Model for the Inhibition of Degradation of Proteoglycan by Small Molecule Compounds: Bovine Articular Cartilage Explant Cultures

This in vitro procedure was used to evaluate the effect and relative potencies of small molecule compounds to inhibit the degradation of proteoglycan in articular cartilage treated with Interleukin 1 alpha (IL-1α). Treatment of articular cartilage with IL 1α induces expression of a cascade of enzymes that initiates cartilage matrix degradation. This assay was utilized to select small molecule compounds that inhibit the enzyme activity and protects cartilage matrix degradation.


Materials and Methods

Articular cartilage: bovine metacarpal joints were disinfected with 10% Wescodyne followed by 70% ethanol. 6 mm articular cartilage discs were prepared and rinsed once in PBS and medium, respectively. The discs were cultured in a stirring flask with serum-free medium for 5 days for equilibrium with one medium change. Small molecule compounds: stock solutions of test small molecule compounds (usually at 5 mg/ml) were prepared in DMSO. Working solutions were diluted with DMSO and generally ranged from 0.1 μg/ml to 10 ug/ml. The DMSO stocks were stored at 20° C. Articular cartilage explant culture medium: DMEM, 2 mM glutamax, 100 U/ml of antibiotic and antimycotic, 50 μg/ml ascorbate and 10 mM Hepes (pH-7.0). Proteoglycan content-Dimethyl methylene blue (DMMB) solution: IL-16 mg DMMB, 10 ml 100% ethanol, 29.5 ml of 1M NaOH, 3.5 ml of 98% formic acid and water to 1 L. Chondroitin sulphate sodium salt: 1.56-200 ug/ml serial dilutions.


For the explant culture, cartilage discs were cultured in serum-free medium for 48 h or more. Discs were weighed and transferred to a 96-well plate, 1 disc per-well. 0.2 ml of medium containing 5 ng/ml of IL-1 and different concentrations of compounds were added to each well, with 3 wells allocated for each treatment. The medium was collected at days 1, 2, & 3 and replaced accordingly.


For the DMMB assay, conditioned medium (CM) samples were diluted with medium. 10 ul of each standard or diluted sample was added in duplicate into a 96-well-plate and 200 μl of DMMB solution was added into each well. Absorbance at 520 nm was measured for each well. Proteoglycan contents in CM were calculated daily.


Results

The proteoglycan contents are expressed as ug per mg of cartilage. The inhibitory effect of the compounds is expressed as percentage. (See, Tortorella, M. D. et al., Science 1999, 284, 1664-1666). The assay results are shown in Tables 1, 2, and 3 below.


















TABLE 1








Agg 2









Agg1 IC50
Ki
IC50


Example
(nM)
(nM)
(nM)
MMP-13
MMP-2
MMP-14
MMP-1
bovine
bovine
























8SSSS
>200,000










7
150

1,800
~13,000
3,000
~13,000
>100,000
61
28


8C
110

1,200
~50,000
6,000
~25,000
>100,000
68
34


8B
460

1,900
~50,000
5,600
~25,000
>100,000
54
22


8A
150

900
~40,000
4,400
~25,000
>100,000
75
29


11
110

~800
~5,000
~1,000
~2,000
~2,000
78
52


12A
700

~5,000
~6,000
~2,000
~2,000
~2,000
41
18


12B
35000


8D
220

2,800
~25,000
4,400
17,000
>100,000
67
27


8E
90

746
~25,000
3,700
~25,000
>200,000
79
34


8NNNNNN
~40,000


13B
~50,000


8F
30
12
304
~22,000
5,630
~25,000
>200,000
81
56


17VV
7900


13A
1500

~67,000
>100,000
~67,000
>100,000
>100,000
37
18


13C
9600


13D
750

~26,000
>200,000
>200,000
>100,000
>100,000
51
25


13E
~25,000


13F
~200


8G
80

614
~50,000
~50,000
~25,000
>100,000
76
39


8OOOOOO
670


8H
200

266
~50,000
3,310
~25,000
>100,000
85
67


8I
500


12E
1800


12C
25000


12D
>100,000


8K
300


8L
55

189
~50,000
10,500
21,500
>100,000
87
79


8J
360


8M
120

856
~50,000
8,000
13,000
>100,000
85
17


8N
290


8O
75
82
1,300
31,400
5,000
~22,000
no inh
69
34


8P
290


8Q
11
31
400
37,300
6,100
~22,000
no inh
66
24


8R
16
5
50
42,400
10,400
~22,000
no inh
86
78


8S
150
245
1,300
>200,000
3,130
~25,000
no inh
51
40


8T
300






51
20


8U
200






63
26


8V
160

1,100
~67,000
3,430
~30,000
>100,000
73
45


8W
75

971
~22,000
~2,500
~25,000
no inh


8X
100

1,100
~67,000
7,000
~25,000
no inh


8Y
50

520
~67,000
5,534
~25,000
no inh


8Z
80

680
~67,000
3,820
~25,000
no inh


8BB
74

650
19,000
2,300
~25,000
no inh


8AA
370


33D
<10

13.4
52
3.3
73.6
~1,560


33C
<10

25
29
4.5
110
1,500


Control
<10

2.6
32
4.7
112
1,750


(33A)


8CC
22
19
121
~22,000
5,655
~25,000
no inh
94
71


13G
~2500


8DD
94

930
~22,000
4,580
16,200
no inh
89
50


14
60% @



16,700


15A
>22,000


15B
67% @



7,500


15C
50% @



7,500


15D
86% @



7,500


15E
3500


33B
<30


8PPPPPP
>33,000


23
>6000


24A
1100


24B
~2200


24C
~300


20
~22,000


21A
2500-7500


21B
~6700


21C
no inh


21D
no inh


22C
>50,000


8FFF
200

1000


8GGG,
100






57
12


step D


8AAAA
100


8DDDD
110


8HHHH
200


8XX
300


8FF
26
28





86
50


17WW
no inh


17XX
no inh


17YY
2000


17ZZ
200


17AAA
no inh


8YY
530

4500


8ZZ
210


8AAA
48
44





83
55


8EE
200


15F
600


15G
2200


15H
200


6A
150

740
>50,000
29800
>200,000
>50,000


8HH
160


8II
44
39





75
40


8QQQQQQ
690


17BBB
900


8III
120


8NNN
100
219
1750
14,000
>10,000
>50,000
>50,000
60
0


8JJJ
54
54
300
30,000
>20,000
>200,000
>200,000
74
26


8JJ
45
33
1100
17,000
27,000
>200,000
>200,000
70
17


10M
43
54
1100
~20,000
>20,000
>200,000
>50,000
67
35


10N
19
28
970
>50,000
7600
>200,000
>200,000
65
24


8OO
<30
6
44
>50,000
>20,000
>200,000
>200,000
87
40


22A
1000


22B
200


8KK
180


8LL
55

990
24,000
50,000
200,000
200,000
40
0


10R
77
187
1900
>20,000
>20,000
>200,000
>50,000
41
0


8BBB
32
130
700
>50,000

no inh
>200,000
35
0


8CCC
100






74
26


8ZZZ
100

1600
no inh
13,000
>200,000
>50,000
72
34


8KKK
120

2000


10C
240

>5000


10E
370


10F
130

>30000


10D
480

3200


10G
1000

4400


8BBBB
100






40
0


8OOO
280

1000


8LLL
230


8PPP
130


8QQQ
330


8EEE
7900


8MM
58
126
980


9
200


10A
840

2100


10J
260


10K
380

>100000


10L
130

2000


10B
150


8MMM
40

500


8RRR
82

680


10H
300


8SSS
64

470
>50,000
10300


10O
470


8UUU
61
60
660
~50,000
8900
>200,000
no inh


10P
700


10Q
98

3000
~50,000

>50,000
no inh


8PP
<30
10
85
~50,000
6000
>66,600
>200,000


8NN
<30
14
133
~50,000
9000
no inh
>200,000


8VVV
30
38
420
~50,000
5600
>66,600
no inh


8TTT
>10000


6C
150


8DDD
300


6B
300


8SS
100

800
~50,000
12400
>66,600
no inh


8TT
300


8UU
200


8WW
70

350
~50,000
13300
>66,600
no inh


8VV
100

1900
~50,000
9100
>66,600
no inh


8XXX
30

930
~50,000
7900
>200,000
no inh


8WWW
36

840
~200,000
7400
>200,000
no inh


8YYY
100

1600
>50,000
9400
>200,000
no inh


8GGGG
30

160
>50,000
18600
no inh
no inh


8FFFF
3600


10I
320


8RRRRRR
3600


8KKKK
<30
4


8LLLL
no inh


8MMMM
47
57


8IIII
<30
9


8JJJJ
>50000


8NNNN
3400


8OOOO
52
60


8PPPP
>50000


8QQQQ
9600


8RRRR
122


8SSSSSS
146


8TTTTTT
17300


1
900


2
111


3
12700


4
1100


5
1800


8EEEE,
400


step D


8UUUUUU
4000


26
5000


27
400


28
400


29
3000


30
500


31
1000


6D
45


6E
56


8VVVVVV
46























TABLE 2








Agg 2







Agg1 IC50

IC50


MMP-


Example
(nM)
Ki (nM)
(nM)
MMP-13
MMP-2
14
MMP-1






















8TTTT
>17,000

>22,000






8UUUU
>17,000

~67,000


8VVVV
>17000

>67,000


8WWWW
8300

>67,000


8XXXX
>50,000

>200,000


8YYYY
1500

>200,000


8ZZZZ
>17000

>22,000


8AAAAA
372

410


8BBBBB
>17000

20900


8CCCCC
107

11000


8DDDDD
315

1100


8EEEEE
>50000

>200,000


8FFFFF
92

710
>100,000


8GGGGG
>50,000


8HHHHH
357


8IIIII
813

1900


8JJJJJ
191

1100


8KKKKK
>50,000

>22,000


8LLLLL
>17,000

>200,000


8MMMMM
20000

~22,000


8NNNNN
1200

1,893 +/− 76  


8OOOOO
>50,000

>200,000


8PPPPP
157

2967


8QQQQQ
108

1165


8RRRRR
129

2905


8SSSSS
208

2470


8TTTTT
389

2011


8UUUUU
317

2119


8VVVVV
221

1558


8WWWWW
>17,000

>67,000


8XXXXX
246

1450


8YYYYY
87

2800
~20,000


8ZZZZZ
<30

63
>100,000


16
<30 nM

467


17A
116

1123


17B
204

1020


18D
4400

>22,000


17C
<30 nM

80


17D
>17,000 nM   

>200,000


17E
251

1040


12F
~3,000 nM    

3,000-8,000


17F
1700

~8,300


17G
1600

3,000-8,000


17H
1000

~22,000


8AAAAAA
534

1759



17I
<30

217 +/− 8 
~100,000


17J
~5,600

~30,000



17K
1200

~3,700



17L
133

226



18E
2200

>11,000



17M
~310

>11,000



17N
40

42
11,200 +/−






1,200


8MMMMMM
550

5100



8BBBBBB
470

2671



18F
>67,000

>67,000



17O
8500

~22,000



17P
126

58



17Q
~13,000

>30,000



17R
73

142
6,000 +/− 400


18
49

50
~1,600 nM


19A
930

1020


19B
3200

590


8CCCCCC
2400

>22000


17S
4600

10100


17T
3200

7200


17U
1800

~6700


19C
140 +/− 20

75


17V
90

1800


17W
>25,000

~67000


17X
2000

~22000


8DDDDDD
2400

>22000


17Y
<30

156


19D
210

223


19E
49

1120


17Z
170

3140


17AA
3500

5700


17BB
3600

2900


17CC
2900

2970


17DD
<30 nM

1190


17EE
<30 nM

194


17FF
690

13900


17GG
34

113


17HH
2200

6320


17II
~1000-3000

~10,000


17JJ
2400

19454


15I
430

1593


15J
510

~3,125


12G
>25000

11700


12H
1200

1635


12I
>25000

>20,000


17KK
>25000

>67,000


19F
68

163


17LL
87

1193


17MM
46

2707


19G
270

146


19H
69

158


18G
~8300


17NN
200

729


8EEEEEE
5000


17OO
<30

1677


8FFFFFF
3900

18131


8GGGGGG
4200


8HHHHHH
38

46


8IIIIII
2900

11908


8JJJJJJ
300

943


8KKKKKK
350

1674


17QQ
>25,000

~22000


12J
~8,000

~22000


12K
~25,000

>22000


12L
~22,000

>22000


17RR
<30

26


17SS
450

3959


17TT
120

173


8LLLLLL
>25000

>100000


15Y
220

841


15Z
1400

6036


15AA
44

327


15BB
53

527


17UU
<30

39

























TABLE 3






Agg1
Ki
Agg 2
Ki








IC50
Agg1
IC50
Agg2
MMP-

MMP-

MMP-


Example
(nM)
(nM)
(nM)
(nM)
13
MMP-2
14
MMP-8
12
























8WWWWWW
<03
7.1
50
72

2600

1000
5800


8XXXXXX
<30
<3
20
60

7500

4100
1600


8YYYYYY
<30
13.5
30
160

4700

737
365


8ZZZZZZ
<30

120


8AAAAAAA
80

550


8BBBBBBB
110

1000


8CCCCCCC
50

680


8DDDDDDD
<30

220


8EEEEEEE
37

510


8FFFFFFF
200

4200


8GGGGGGG
60

160


8HHHHHHH
50

180


8IIIIIII
<30

42


8JJJJJJJ
<30

53


8KKKKKKK
<30

<30


8LLLLLLL
228

3380


8MMMMMMM
198

614


8NNNNNNN
<30
<3
<30
37

2150

1210
680


8OOOOOOO
<30

100-400


8PPPPPPP
3116

48%





16700


8QQQQQQQ
NT

NT


8RRRRRRR
70-210

48%





1200


8SSSSSSS
57
22
230
356


8TTTTTTT
707

20%





11000


8UUUUUUU
<30
<3
60
60

5180

2030
410


8VVVVVVV
<30
7
51% 140
305

570

410
770


8WWWWWWW
<30
<3
50
42

1080

370
630


8XXXXXXX
<30
4.5
20-70
56


8YYYYYYY
<30
27
200-600
197


8ZZZZZZZ
<30
12.7
200-600
290


8AAAAAAAA
<30
86
 600-1800
1147


8BBBBBBBB
300

200-600


8CCCCCCCC
140

5200


8DDDDDDDD
50

1000


8EEEEEEEE
<30
3.8
 60-200
79


8FFFFFFFF
<30

200


8GGGGGGGG
40

3400


8HHHHHHHH
60

3000


8IIIIIIII
65%

 90-270



200


8JJJJJJJJ
<30

 800-2500


8KKKKKKKK
70

270-800


8LLLLLLLL
<30
<3
 60-200
156


8MMMMMMMM
<30
<3
 60-200
38


8NNNNNNNN
<30
<3
 70-200
151


8OOOOOOOO
<30

200-600


8PPPPPPPP
<30

200-600


8QQQQQQQQ
<30

3300


8RRRRRRRR
57% 70

7200


8SSSSSSSS
<30
5.4
 70-200
51


8TTTTTTTT
20-70
37.6
100-300
359


8UUUUUUUU
100

700


8VVVVVVVV
60%

50%



16700

50000


8WWWWWWWW
20-70
40.6
100-300
526


8XXXXXXXX
100

1700


8YYYYYYYY
<30
6.2
100-300
143


8ZZZZZZZZ
20-70

9900


8AAAAAAAAA
<30
6.7
98
45


8BBBBBBBBB
<30
11.5
100-300
681


8CCCCCCCCC
<30

900


8DDDDDDDDD
2400

50%





7400


8EEEEEEEEE
1200

50%





2500


8FFFFFFFFF
270

62%





25000


8GGGGGGGGG
30%

28%



8300

25000


8HHHHHHHHH
<30

500


8IIIIIIIII
300-900

5% 2500


8JJJJJJJJJ
25%

10%



25000

25000


8KKKKKKKKK
16%

10%



25000

25000


8LLLLLLLLL
<30

>300


8MMMMMMMMM
<30
<3
 30-100
87


8NNNNNNNNN
<30
8.3
 30-100
69


8OOOOOOOOO
<30
2.7
~30
66

718

349
372


8PPPPPPPPP
<30
11.5
 30-300
126

7240

2550
2890


8QQQQQQQQQ
>900

>8300


8RRRRRRRRR
>8300

100000


8SSSSSSSSS
<30
8.8
200
157


8TTTTTTTTT
<30
6.7
59
54


8UUUUUUUUU
<30
6.9
83
75


8VVVVVVVVV
<30
4.5
77
81


8WWWWWWWWW
>25000

>100000


8XXXXXXXXX
<30
18.2
25
71.6

2610

1790
597


8YYYYYYYYY
<30
39.6
250
349

25500

13200
3710


8ZZZZZZZZZ
<30
4.7
30-90
123

8280

2440
2860


8AAAAAAAAAA
<30
28.9
100-300
204

6970

2230
2240


8BBBBBBBBBB
22000

>200000


8CCCCCCCCCC
1040

7400


8DDDDDDDDDD
<30
27.2
90
218

4000

763
628


8EEEEEEEEEE
<30
23.2
80
180

6400

2500
1070


8FFFFFFFFFF
>400

>2700


8GGGGGGGGGG
<30
42
>300
723

1300

>5000
>10,000


8HHHHHHHHHH
<30
11.7
>100
405

8000

3400
>10,000


8IIIIIIIIII


15O
52

3132


15P
<30

255


15K
<30

379


15L
47

413


15M
<30

789


15N
91

103


15U
53%

600



100


15V
47% 40

700


15Q
158

1939


15R
560

48%





11000


15S
30%

41%



1850

33000


15U
53%

30%



1000

11000


15W
56%

59%



1900

11000


15X
46%

52%



1900

11000









Example 40
Aggrecanase Inhibitors in Human Osteoarthritic Cartilage Explant Studies and Effect on Aggrecan Metabolism in Human Osteoarthritic Cartilage

The loss of cartilage matrix in osteoarthritis (OA) is associated with increased loss of type II collagen by collagenases and aggrecan loss by aggrecanases. Depletion of aggrecan is one of the earliest changes observed in osteoarthritis (Lohmander, L S et al., Arthritis & Rheum 1993, 36, 1214-1222). Aggrecan is a chondroitin sulfate and keratan sulfate-bearing proteoglycan. Aggrecanase 1 and Aggrecanase 2 cleave aggrecan within the interglobular domain at the aggrecanase site between residues Glu373 and Ala374 (Sandy, J D et al., J Clin Invest. 1992, 89, 1512-1516). Aggrecan cleavage by aggrecanase results in the release of a large sulfated glycosaminoglycan (sGAG)-containing aggrecan fragments which diffuse out of the cartilage matrix. Neoepitope antibodies have been generated to recognize these aggrecanase generated aggrecan fragments (Hughes, C E et al., Biochem J. 1995, 305, 799-804). Aggrecan fragments in inflammatory and OA synovial fluid have been reported as being generated by cleavage at the aggrecanase site (Malfait, A-M et al., J Biol Chem. 2002, 277, 22201-22208). Inhibiting aggrecanase activity is an attractive therapeutic target for OA. In this example, we investigated the effects of a selective aggrecanase inhibitor on the fate of existing and newly synthesized aggrecan in human OA cartilage.


Materials and Methods

Human OA Cartilage Explant Culture: Discarded tissues from consented knee replacement patients were obtained from New England Baptist Hospital (Brookline, Mass.) in sterile phosphate buffered saline (PBS) after about 2-4 hrs post surgery. Cartilage slices were harvested from the knee specimens and washed several times in PBS. These cartilage slices were cut into appropriate pieces (6-8 mm) and rinsed in PBS and then in media and further cultured for additional days as cartilage explants. The medium consisted of Dulbecco's Modified Eagle's medium (DMEM, JRH Biosciences, Lenexa, Kans.), 50 μg/ml ascorbic acid (Wako, Osaka, Japan), 10 mM HEPES, pH 7.0 (Mediatech, Herndon, Va.), 2 mM L-glutamine (Mediatech), 100 U/ml antibiotic-antimycotic solution (Mediatech).


Test compounds: The test compounds are described in Examples 8OO, 8NNNNNNN, and 8WWWWWWW. Stock solutions of the compounds were prepared at 1 mg/ml in DMSO, aliquoted and stored at −20° C. Working solutions of the compounds were made in DMSO and added to the culture for efficacy studies.


Efficacy study: Cartilage explants were cultured for 2-3 days in DMEM medium in a 37° C. and 5% CO2 environment to equilibrate the tissue. Explants were weighed (˜250 mg) and placed in wells of a 24-well culture dish in 1 ml of medium and 3 replicates per treatment group. For the efficacy study, cartilage explants were cultured for 6 days in the presence or absence of various concentrations of the test compound. The media was replaced on day 3 and the experiment was terminated on day 6. Total aggrecan content of conditioned media was measured by a colorimetric assay with DMMB (Farndale, R W et al., Biochim Biophys Acta. 1986, 883, 173-177).


Cartilage aggrecan metabolism study: Cartilage metabolism study was performed with test compound 8OO. Cartilage explants immediately after harvest were cultured in the presence or absence of test compound 8OO. On day 3, media was collected and replaced with media, test compound and labeled sulfate (35SO4,5 mCi, Perkin Elmer, Boston, Mass.). On day 6, media was collected, explants washed and replaced with media and test compound 8OO. On Day 9, media was collected, explants washed and the remaining tissue was digested with Proteinase K (Sigma). Samples were separated on NAP10 columns (Pharmacia, Uppsala, Sweden) to separate free label and newly synthesized labeled aggrecan. Labeled aggrecan molecules were counted using Opti-Fluor (Perkin Elmer) and a liquid scintillation-measuring instrument (Beckman Coulter, Fullerton, Calif.). The percentage of newly synthesized aggrecan in cartilage and media was estimated.


Western analysis: Aggrecan fragments in conditioned medium were analyzed by western analysis using neoepitope antibody, BC-3 designed to recognize the Aggrecanase-generated N-terminal interglobular neoepitope 374ARGS. Equal cartilage weight equivalent conditioned media from each replicate well per treatment group was pooled and digested with Chondroitinase ABC (Sigma, St Louis, Mo.), Keratinase I (Seikagaku America, Falmouth, Mass.) and Keratinase II (Seikagaku America, Falmouth, Mass.) for 2 h at 37° C. Samples were concentrated by YM-10 centrifugal filter device (Millipore Corp., Bedford, Mass.), lyophilized and reconstituted with equal volume of water. Samples were separated under reducing conditions on 4-12% gradient Tris-glycine gels (Invitrogen, Carlsbad, Calif.). Proteins from the gels were transferred to nitrocellulose membranes. Immunoblots were probed using Mab BC-3 (Abcam, Cambridge, Mass.). Incubation with primary and alkaline-phosphatase-conjugated secondary goat anti-mouse IgG (Promega Corp., Madison, Wis.) was performed overnight at 4° C. and at room temperature for 1 h, respectively. The immunoblots were incubated with NBT/BCIP substrate (Promega Corp., Madison, Wis.) at room temperature to achieve optimum color development. The aggrecan fragments containing the ARGS neoepitope were quantitated by densitometry analysis and the percentage inhibition of aggrecan degradation by test compounds was determined.


Results

Efficacy of test compounds 8OO, 8NNNNNNN, and 8WWWWWWW: Information on human donors including age and sex are shown in Table 4b, 5b, and 6b. Cartilage from all the donors when placed in culture exhibited Aggrecanase-mediated aggrecan cleavage and when treated with test compounds showed dose dependent inhibition of Aggrecanase-mediated aggrecan cleavage. Results of the inhibition of aggrecanase activity in terms of inhibition of aggrecanase generated aggrecan fragments by test compounds are shown in Table 4a, 5a, and 6a. Based on these results, EC50 of test compound 8OO was determined to be about 150 ng/ml (FIG. 1), EC50 for test compound 8WWWWWWW was around 40-160 ng/ml; and EC50 for test compound 8NNNNNNN was less than 40 ng/ml. However, test compound 8WWWWWWW and test compound 8NNNNNNN were tested in only 8 and 4 donors, respectively. Test compound 8OO was tested in a total of 15 donors.


Effect of test compound 8OO on Aggrecan Metabolism: Articular cartilage from 3 human OA donors was analyzed for the effect of test compound 8OO on newly synthesized aggrecan. Cartilage from all 3 donors showed the ability to synthesize new aggrecan molecules that was either in the cartilage matrix or released into conditioned medium. Cartilage from all 3 donors when treated with compound 8OO showed dose dependent increase in newly synthesized aggrecan content of the cartilage matrix and an equivalent decrease in aggrecan released into the medium (FIG. 2).


Aggrecanase activity is a characteristic of human osteoarthritis throughout the disease process. Analysis of aggrecan released from human osteoarthritic articular cartilage revealed significant ongoing degradation of aggrecan by Aggrecanases in human donors tested. Inhibition of Aggrecanase activity by Aggrecanase selective inhibitors reduced release of aggrecan degradation products from the articular cartilage matrix.


Since newly synthesized aggrecan appeared to be susceptible to degradation by Aggrecanases, selective Aggrecanase inhibition resulted in a net increase in aggrecan incorporation into the cartilage matrix. These results indicate that inhibition of Aggrecanases will reduce aggrecan degradation in human osteoarthritic articular cartilage throughout the disease process, and also result in a net increase in extracellular matrix aggrecan.









TABLE 4







A) Efficacy of test compound 8OO in human explant studies


Compound 8OO (BC3 analysis)









Conc. of




compound
Average of 15 donors









(ug/ml)
% inhibition
SDM





0.02
8.52


0.04
26.16
5.33


0.08
42.93
7.86


0.15
49.03
7.87


0.3 
56.40
5.95


0.6 
73.92
5.24


1.25
83.36
5.59


2.5 
100.94
4.12


5  
98.88
1.32


10   
101.60
0.99










B) Human donor information for test compound 8OO studies


Compound 8OO (Donor info)










Sex
Age







F
65



M
83



M
70



F
56



F
76



M
53



F
77



F
74



F
75



M
79



M
61



F
57



M
72



M
70



M
62







~EC50 = 150 ng/ml













TABLE 5







A) Efficacy of test compound 8WWWWWWW


in human explant studies


Compound 8WWWWWWW (BC3 analysis)









Conc. of




compound
Average of 8 donors









(ug/ml)
% inhibition
SDM





0.01
2.85
2.85


0.02
46.39
15.95


0.04
23.50
10.57


0.08
76.91
5.99


0.15
88.47
3.25


0.3 
95.03
1.70


0.6 
98.03
0.90


1.25
100.30
0.55










B) Human donor information for test compound


8WWWWWWW studies


Test compound (Donor info)










Sex
Age







F
69



F
69



F
63



M
60



M
57



F
72



F
65



F
75







~EC50 = 40-160 ng/ml













TABLE 6







A) Efficacy of test compound 8NNNNNNN in human explant studies


Test compound 8NNNNNNN (BC3 analysis)









Conc. of




compound
Average of 4 donors









(ug/ml)
% inhibition
SDM





0.04
70.22


0.08
84.46


0.15
66.74
10.97


0.3 
73.14
19.73


0.6 
98.93
0.92


1.25
99.43
1.44


2.5
98.66
2.20


5  
100.21
1.15


10   
101.15
0.42










B) Human donor information for test compound 8NNNNNNN studies


Test compound (Donor info)










Sex
Age







M
63



F
68



F
72



F
60







~EC50 = less than 40 ng/ml






While particular embodiments of the present invention have been illustrated and described, it would be obvious to those skilled in the art that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims
  • 1-35. (canceled)
  • 36. A compound of the formula (I):
  • 37. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein W is —CO.
  • 38. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein R3 is —CO2H.
  • 39. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein m is 0 and p is 2.
  • 40. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein R4 is —CO2H, —CONH2, —(CH2)nOR7, or —CONR9R10, wherein R7, R9, R10, and n are defined as in claim 36.
  • 41. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein W is —CO,R2 is hydrogen,R3 is —CO2H,R4 is —CONR9R10,p is 2, andR5, R6, R9, and R10 are defined as in claim 36.
  • 42. The compound or pharmaceutically acceptable salt of the compound of claim 36, wherein the compound or pharmaceutically acceptable salt of the compound is the S-enantiomer with respect to the carbon to which R4 is bound.
  • 43. The compound of claim 36 of the formula (Ia):
  • 44. The compound of claim 36 of the formula (Id):
  • 45. The compound of claim 44 of the formula (Id-1):
  • 46. The compound of claim 44 or a pharmaceutically acceptable salt thereof, whereinR2 is hydrogen;R4 is
  • 47. The compound of claim 46 or a pharmaceutically acceptable salt thereof, wherein R13 is halogen.
  • 48. A compound selected from:
  • 49. A composition comprising the compound or a pharmaceutically acceptable salt of the compound of claim 36 and a pharmaceutically acceptable carrier.
  • 50. The composition of claim 49, wherein the pharmaceutically acceptable carrier is suitable for oral administration and the composition comprises an oral dosage form.
  • 51. A method for treating a disorder, in an animal in need thereof, a disorder selected from the group consisting of arthritic disorders, osteoarthritis, cancer, rheumatoid arthritis, asthma, chronic obstructive pulmonary disease, atherosclerosis, age-related macular degeneration, myocardial infarction, corneal ulceration and other ocular surface diseases, hepatitis, aortic aneurysms, tendonitis, central nervous system diseases, abnormal wound healing, angiogenesis, restenosis, cirrhosis, multiple sclerosis, glomerulonephritis, graft versus host disease, diabetes, inflammatory bowel disease, shock, invertebral disc degeneration, stroke, osteopenia, and periodontal diseases, wherein the method comprises administering an effective dose of the compound of claim 36, or a pharmaceutically acceptable salt or hydrate thereof, wherein the metalloproteinase-related.
  • 52. The method of claim 55, wherein the disorder is osteoarthritis.
CROSS-REFERENCE TO RELATED APPLICATIONS

This is a Divisional Application of U.S. Ser. No. 11/484,005, filed Jul. 11, 2006, which claims the benefit under 35 U.S.C. § 119(e) of U.S. Ser. No. 60/697,590, filed Jul. 11, 2005, all of which are incorporated by reference herein in their entirety.

Provisional Applications (1)
Number Date Country
60697590 Jul 2005 US
Divisions (1)
Number Date Country
Parent 11484005 Jul 2006 US
Child 12465840 US