Claims
- 1. A method for selectively controlling weeds in a field containing a crop of planted crop seeds or plants which method comprises the steps of:
- a) planting said crop seeds or plants which are glyphosate resistant as a result of a chimeric gene being inserted into said crop seed or plant, said chimeric gene having
- i) a promoter sequence which functions in plant cells,
- ii) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell, and
- iii) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA,
- where the promoter is heterologous with respect to the coding sequence and adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with said gene; and
- b) applying to said crop and weeds in said field a sufficient amount of glyphosate to control said weeds without significantly affecting said crop.
- 2. The method of claim 1 wherein said promoter sequence is a plant virus promoter sequence.
- 3. The method of claim 2 wherein said promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
- 4. The method of claim 3 wherein said promoter sequence is the CaMV35S promoter sequence.
- 5. The method of claim 1 in which the coding sequence encodes a glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
- 6. The method of claim 1 in which the EPSPS coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
- 7. The method of claim 1 wherein said chloroplast transit peptide is from a plant EPSPS gene.
- 8. A glyphosate-resistant dicotyledonous plant seed, said seed comprising a chimeric plant gene having:
- i) a promoter sequence which functions in plant cells;
- ii) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell; and
- iii) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA,
- where the promoter is heterologous with respect to the coding sequence and adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with said gene.
- 9. The glyphosate-resistant dicotyledonous plant seed of claim 8 wherein said promoter sequence is a plant virus promoter sequence.
- 10. The glyphosate-resistant dicotyledonous plant seed of claim 9 wherein said promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
- 11. The glyphosate-resistant dicotyledonous plant seed of claim 10 wherein said promoter sequence is the CaMV35S promoter sequence.
- 12. The glyphosate-resistant dicotyledonous plant seed of claim 8 wherein said coding sequence encodes a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
- 13. The glyphosate-resistant dicotyledonous plant seed of claim 8 wherein said coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
- 14. The glyphosate-resistant dicotyledonous plant seed of claim 8 wherein said chloroplast transit peptide is from a plant EPSPS gene.
- 15. A glyphosate-resistant tomato seed of claim 8.
- 16. A glyphosate-resistant tobacco seed of claim 8.
- 17. A glyphosate-resistant oil seed rape seed of claim 8.
- 18. A glyphosate-resistant flax seed of claim 8.
- 19. A glyphosate-resistant soybean seed of claim 8.
- 20. A glyphosate-resistant sunflower seed of claim 8.
- 21. A glyphosate-resistant sugar beet seed of claim 8.
- 22. A glyphosate-resistant alfalfa seed of claim 8.
- 23. A glyphosate-resistant cotton seed of claim 8.
Parent Case Info
This application is a continuation-in-part application of and claims priority from our copending application Ser. No. 06/879,814, filed Jul. 7, 1989 now U.S. Pat. No. 4,940,835, which is a continuation-in-part of application Ser. No. 792,390, filed Oct. 29, 1985 abandoned, which, in turn, is a continuation-in-part of application Ser. No. 763,482, filed Aug. 7, 1985 abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4940835 |
Shah et al. |
Jul 1990 |
|
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
879814 |
Jul 1986 |
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Parent |
792390 |
Oct 1985 |
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Parent |
763482 |
Aug 1985 |
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