Claims
- 1. A method for producing a gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which comprises altering a gene encoding EPSP synthase to cause the substitution of an alanine residue for the second glycine residue in the amino acid sequence:
- -L-G-N-A-G-T-A-
- located between positions 80 and 120 in a mature EPSP synthase sequence.
- 2. A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a plant EPSP synthase gene.
- 3. A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a bacterial EPSP synthase gene.
- 4. A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a fungal gene encoding EPSP synthase.
- 5. A glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which contains the amino acid sequence:
- -L-G-N-A-A-T-A-
- between positions 80 and 120 in the mature EPSP synthase sequence.
- 6. The glyphosate-tolerant EPSP synthase enzyme of claim 5 as shown in FIGS. 1(a) and 1(b).
- 7. A glyphosate-tolerant EPSP synthase gene produced by the method of claim 1 wherein the wild-type EPSP synthase coding sequence is selected from the group of EPSP synthases consisting of Aspergillus nidulans, petunia, tomato, maize, Arabidopsis thaliana, Glycine max, E. coli K-12 and Salmonella typhimurium as shown in FIG. 2.
- 8. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Aspergillus nidulans.
- 9. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from petunia.
- 10. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from tomato.
- 11. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from maize.
- 12. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Arabidopsis thaliana.
- 13. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Glycine max.
- 14. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from E. coli K-12.
- 15. A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Salmonella typhimurium.
Parent Case Info
This application is a continuation-in-part of co-pending application Ser. No. 054,337, filed May 26, 1987, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4769061 |
Comai |
Sep 1988 |
|
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
54337 |
May 1987 |
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