Claims
- 1. A plant gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the amino acid sequence:
- -L-G-N-A-A-T-A-
- between positions 80 and 120 in the mature EPSP synthase sequence.
- 2. A DNA molecule encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the amino acid sequence:
- -L-G-N-A-A-T-A-
- between positions 80 and 120 in the mature EPSP synthase sequence.
- 3. A DNA molecule of claim 2 which is less than twenty kilobases in length.
- 4. A DNA molecule of claim 3 encoding a glyphosate-tolerant EPSP synthase which contains the amino acid sequence:
- -L-G-N-A-A-T-A-
- between positions 80 and 120 in the mature EPSP synthase sequence as shown in FIG. 1.
- 5. A method of using a planted crop seed or plant which is glyphosate tolerant to selectively control weeds in a field containing said planted crop seed or plant, said method comprising the steps of:
- a) planting said crop seed or plant which is glyphosate tolerant as a result of a chimeric gene being inserted into said crop seed or plant, said chimeric gene having
- i) a promoter sequence which functions in plant cells,
- ii) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell and said glyphosate-tolerant EPSP synthase has the sequence:
- -L-G-N-A-A-T-A-
- between positions 80 and 120 of the mature EPSP synthase sequence, and
- iii) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of multiple adenine nucleotides to the 3' end of the RNA,
- where the promoter is adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with said gene; and
- b) applying to said crop and weeds in said field a sufficient amount of glyphosate to control said weeds without significantly affecting said crop.
- 6. THe method of claim 5 wherein said gene encoding a glyphosate-tolerant EPSP synthase enzyme is from a plant source.
Parent Case Info
This application is a divisional of and claims priority from co-pending application Ser. No. 550,267 filed Jul. 9. 1990, now U.S. Pat. No. 5,145,783, which is a continuation-in-part of application Ser. No. 179,245, filed Apr. 22, 1988, now U.S. Pat. No. 4,971,908, which is a continuation-in-part of application Ser. No. 054,337, filed May 26, 1987, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4769061 |
Comai |
Sep 1988 |
|
4971908 |
Kishore et al. |
Nov 1990 |
|
5145783 |
Kishore et al. |
Sep 1992 |
|
Divisions (1)
|
Number |
Date |
Country |
Parent |
550267 |
Jul 1990 |
|
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
179245 |
Apr 1988 |
|
Parent |
54337 |
May 1987 |
|