Claims
- 1. An isolated DNA molecule selected from the group of:
a promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues and a promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 2. The isolated DNA molecule according to claim 1, wherein the DNA molecule is the promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 3. The isolated DNA molecule according to claim 2, wherein the chlorophyllous tissue is leaf tissue.
- 4. The isolated DNA molecule according to claim 2, wherein the isolated DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 5. The isolated DNA molecule according to claim 1, wherein the DNA molecule is the promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues.
- 6. The isolated DNA molecule according to claim 5, wherein the embryonic seed tissue is a cotyledon.
- 7. The isolates DNA molecule according to claim 5, wherein the isolated DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 8. A chimeric gene comprising:
a promoter region comprising a first DNA molecule according to claim 1;a coding region operably linked 3′ to the promoter region, the coding region comprising a second DNA molecule encoding an mRNA molecule or a protein or polypeptide; and a 3′ regulatory region operably linked 3′ of the coding region.
- 9. The chimeric gene according to claim 8, wherein the first DNA molecule is a promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 10. The chimeric gene according to claim 9, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 11. The chimeric gene according to claim 9, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of an herbicide resistance protein or polypeptide, a pest or pathogen resistance protein or polypeptide, pathogen-derived proteins, antibodies, and vaccines.
- 12. The chimeric gene according to claim 8, wherein the first DNA molecule is a promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues.
- 13. The chimeric gene according to claim 12, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 14. The chimeric gene according to claim 12, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of a protein which modifies amino acid content in seed tissues, a protein which modifies fatty acid content of seed tissues, and a protein or polypeptide which modifies gossypol content.
- 15. An expression system comprising an expression vector in which is inserted a chimeric gene according to claim 8.
- 16. A host cell comprising a chimeric gene according to claim 8.
- 17. The host cell according to claim 16, wherein the host cell is a plant cell or a bacteria cell.
- 18. The host cell according to claim 17, wherein the bacteria cell is Agrobacterium.
- 19. The host cell according to claim 17, wherein the plant cell is a cell of a plant selected from the group consisting of rice, wheat, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, strawberry, grape, raspberry, pineapple, soybean, tobacco, tomato, sorghum, sugarcane, poplar, rubber, Paulownia, pine, and elm.
- 20. A transgenic plant comprising a chimeric gene according to claim 8.
- 21. The transgenic plant according to claim 20, wherein the first DNA molecule is a promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues.
- 22. The transgenic plant according to claim 21, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 23. The transgenic plant according to claim 21, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of a protein which modifies amino acid content in seed tissues, a protein which modifies fatty acid content of seed tissues, and a protein or polypeptide which modifies gossypol content.
- 24. The transgenic plant according to claim 20, wherein the first DNA molecule is a promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 25. The transgenic plant according to claim 24, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 26. The transgenic plant according to claim 24, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of an herbicide resistance protein or polypeptide, a pest or pathogen resistance protein or polypeptide, pathogen-derived proteins, antibodies, and vaccines.
- 27. The transgenic plant according to claim 20, wherein the plant is selected from the group consisting of rice, wheat, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, strawberry, grape, raspberry, pineapple, soybean, tobacco, tomato, sorghum, sugarcane, poplar, rubber, Paulownia, pine, and elm.
- 28. The transgenic plant according to claim 12, wherein the plant is a cotton plant.
- 29. A plant seed obtained from the transgenic plant of claim 20.
- 30. The plant seed according to claim 29, wherein the transgenic plant is selected from the group consisting of rice, wheat, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, strawberry, grape, raspberry, pineapple, soybean, tobacco, tomato, sorghum, sugarcane, poplar, rubber, Paulownia, pine, and elm.
- 31. The plant seed according to claim 29, wherein the transgenic plant is cotton.
- 32. A transgenic plant seed comprising a chimeric gene according to claim 8.
- 33. The transgenic plant seed according to claim 32, wherein the first DNA molecule comprises a promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues.
- 34. The transgenic plant seed according to claim 33, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 35. The transgenic plant seed according to claim 33, wherein the embryonic seed tissue is a cotyledon.
- 36. The transgenic plant seed according to claim 33, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of a protein which modifies amino acid content in seed tissues, a protein which modifies fatty acid content of seed tissues, and a protein or polypeptide which modifies gossypol content.
- 37. The transgenic plant seed according to claim 32, wherein the first DNA molecule comprises a promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 38. The transgenic plant seed according to claim 37, wherein the first DNA molecule comprises a:nucleotide sequence of SEQ ID No: 1.
- 39. The transgenic plant seed according to claim 37, wherein the second DNA molecule encodes a protein or polypeptide selected from the group consisting of an herbicide resistance protein or polypeptide, a pest or pathogen resistance protein or polypeptide, pathogen-derived proteins, antibodies, and vaccines.
- 40. The transgenic plant seed according to claim 32, wherein the transgenic plant seed is from a plant selected from the group consisting of rice, wheat, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, chicory, lettuce, endive, cabbage, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant, pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, strawberry, grape, raspberry, pineapple, soybean, tobacco, tomato, sorghum, sugarcane, poplar, rubber, Paulownia, pine, and elm.
- 41. The transgenic plant seed according to claim 32, wherein the transgenic plant seed is from a cotton plant.
- 42. A method of making a transgenic plant comprising:
transforming a plant cell or tissue with a chimeric gene according to claim 8; and regenerating a transgenic plant from the transformed plant cell or tissue.
- 43. The method according to claim 42, wherein said transforming is carried out under conditions effective to insert the chimeric gene into the genome of the transformed plant cell or tissue.
- 44. The method according to claim 42, wherein said transforming is Agrobacterium mediated.
- 45. The method according to claim 42, wherein said transforming comprises:
propelling particles at a plant cell under conditions effective for the particles to penetrate into the cell interior and introducing an expression vector comprising the chimeric gene into the plant cell interior.
- 46. The method according to claim 42, wherein the first DNA molecule comprises a promoter-effective DNA molecule of Gossypium which is operable in chlorophyllous tissues.
- 47. The method according to claim 46, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 48. The method according to claim 42, wherein the first DNA molecule is a promoter-effective DNA molecule of Gossypium which is operable in embryonic seed tissues.
- 49. The method according to claim 48, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 50. A method of expressing a heterologous mRNA molecule or protein or polypeptide in chlorophyllous tissue of plants, said method comprising:
transforming a plant cell or tissue with a chimeric gene according to claim 9 and regenerating a plant from the transformed plant cell or tissue, wherein expression of the mRNA molecule or protein or polypeptide occurs in chlorophyllous tissue of the plant.
- 51. The method according to claim 50, wherein said transforming is carried out under conditions effective to insert the DNA construct into the genome of the plant cell or tissue.
- 52. The method according to claim 50, wherein said transforming is Agrobacterium mediated.
- 53. The method according to claim 50, wherein said transforming comprises:
propelling particles at a plant cell under conditions effective for the particles to penetrate into the cell interior and introducing an expression vector comprising the chimeric gene into the plant cell interior.
- 54. The method according to claim 50, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 55. The method according to claim 50, wherein the chlorophyllous tissue is leaf tissue.
- 56. A method of expressing a heterologous mRNA molecule or protein or polypeptide in embryonic seed tissues comprising:
providing a plant seed according to claim 33 and propagating the plant seed under conditions effective to yield a transgenic plant which expresses the mRNA molecule or the protein or polypeptide in embryonic seed tissues.
- 57. The method according to claim 56, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 2.
- 58. The method according to claim 56, wherein the embryonic seed tissue is a cotyledon.
- 59. A method of expressing a heterologous mRNA molecule or protein or polypeptide in chlorophyllous tissues comprising:
providing a plant seed according to claim 37 and propagating the plant seed under conditions effective to yield a transgenic plant which expresses the mRNA molecule or the protein or polypeptide in chlorophyllous tissues.
- 60. The method according to claim 59, wherein the first DNA molecule comprises a nucleotide sequence of SEQ ID No: 1.
- 61. The method according to claim 59, wherein the chlorophyllous tissue is leaf tissue.
Parent Case Info
[0001] This application claims the priority benefit of U.S. Provisional Patent Application Serial No. 60/223,496, which is hereby incorporated by reference in its entirety.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US01/24846 |
8/7/2001 |
WO |
|