Patent Grant
11066379
The phylum Porifera (sponges) presents the most ancient metazoan life forms. They have a simple body plan and a limited number of cell types, and often carry a high load of bacterial symbionts or commensals. The diversity of natural products produced by sponges and associated microorganisms is very high containing members of all known biosynthetic classes. Because of this sponges are the most heavily investigated marine invertebrate phylum, and several sponge compounds or their derivatives are in the clinic or in advanced stages of clinical trials.
Previous investigations of Spongionella species showed it to contain different biosynthetic classes of natural product: diterpenes, nor- and bisnorditerpenes, polyhydroxylated sterols, and furanosesterterpenes. The gracilins, including the rare nor-diterpene gracilin A, was originally isolated and characterized from the Mediterranean sponge Spongionella gracilis. The gracilins, are considered structurally unique owing to the unusual diacetoxyhexahydrodifuro[2,3-b;3′,2′-d]furan-2-one core. The cytotoxic activity of gracilins B, G-I isolated from Spongionella pulchella against a diverse panel of 12 human cancer cell lines has been reported, but these compounds did not progress further in preclinical evaluation. These diterpenes are structurally unique owing to the unusual diacetoxy furanose found in most members. The cytotoxic activity of gracilins B, G-I isolated from Spongionella pulchella against a diverse panel of 12 human cancer cell lines has been reported, but these compounds did not progress further in preclinical evaluation. The absolute configuration of gracilin A was never established, while its relative configuration was determined by X-ray analysis of the keto derivative of 9,11-dihydrogracilin A (3). Gracilin A was reported to be a potent inhibitor of phospholipase A2 (PLA2) with a 69% inactivation efficiency. It has been reported that gracilin A is mildly cytotoxic against K562 and PBMC cells, with IC50 values of 0.6 and 0.8 M, respectively, and inhibited EGF-R by 70% at 100 μM concentration. In addition, it was shown that gracilin A mimics the effects of cyclosporin A (CsA) through inhibition of cyclophilin A (CypA) and improves Alzheimer's Disease (AD) hallmarks in vitro and in vivo.
Treatments for neurodegenerative diseases are a challenge for pharmaceutical development given the multiple and highly interconnected cellular processes implicated in neurodegeneration. For example, oxidative stress (OS), an imbalance between the release of reactive oxygen species (ROS) and cellular antioxidant defenses, has been detected in the early stages of Alzheimer's Disease (AD). Neurons are sensitive to OS due to their high oxygen consumption rate, low antioxidant capacity, and high level of polyunsaturated fatty acids. The early role of neuronal OS in AD has been linked to amyloid beta protein (Aβ) formation, mitochondrial dysfunction, microglial activation and τ-hyperphosporylation, and has led to the study of antioxidants now in Phase I or II clinical trials, such as N-acetyl-L-cysteine. The cyclophilins (Cyp) are highly conserved peptidyl-prolyl, cis-trans isomerases (PPIase) involved in protein folding and trafficking and are found in multiple cellular compartments. Cyclophilin A (CypA), the cytoplasmic isoform, has several roles in cell metabolism and energy homeostasis including the calcineurin-mediated activation of pro-inflammatory genes that encode cytokines with enhanced expression in inflammation and cancer. The use of small molecules that selectively block the inflammation-related functions of CypA, while leaving other functions unaffected, is an important pharmacological strategy leading to effective immunosuppressive agents. Cyclophilin D is a mitochondrial isoform that translocates to form the mitochondrial permeability transition pore (mPTP) and this activity correlates with mitochondrial dysfunction observed in AD leading to neuronal death. Furthermore, mitochondrial amyloid-beta protein (AP) has been implicated in CypD-mediated formation of PTP which leads to ROS generation, intracellular calcium concentration disruption, release of pro-apoptotic factors and ultimately cell death. CypD deficiency was correlated to resistance to both Aβ and Ca2+-induced mitochondrial swelling and permeability and enhanced cognition and memory in an AD mouse model. Thus, small molecules that selectively inhibit the activity of CypD may be used for AD. CsA does not display the desired selectivity for CypD and this large macrocyclic peptide is not blood-brain barrier permeable. Therefore, CypD inhibitors devoid of immunosuppressive activity through binding to CypA but with the desired mitochondrial effect and BBB permeability could be an approach to AD.
Despite the advances in the development of immunosuppressive and neuroprotective agents noted above, a need exists for simplified gracilin A derivatives with high affinity for CypA with utility as immunosuppressants and other derivatives that demonstrate significant selectivity between CypA and CypD demonstrating their potential as neuroprotective agents devoid of immunosuppressive effects. The present invention seeks to fulfill this need and provides further related advantages.
The present invention provides gracilin A congeners and methods for using the gracilin A congeners as immunosuppressive and neuroprotective agents.
In one aspect, the invention provides gracilin A congeners. In certain embodiments, the invention provides a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl;
R4 is H, OH, OAc, or absent;
R5 and R8 are independently H or OAc;
R6 and R7 are H or R6 and R7, taken together, are O; and
the dashed line denotes a bond or absence of a bond with the proviso that only one dashed line is a bond, and with a further proviso that both dashed lines denote absence of a bond when R4 is H, OH, or OAc.
In another aspect of the invention, methods for suppressing an immune response in a subject are provided. In certain embodiments, the invention provides a method for suppressing an immune response in a subject, the method comprising administering to a subject in need thereof an effective amount of a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl, and the dashed line denotes a bond or an absence of a bond, with the proviso that only one dashed line is a bond.
In a further aspect, methods for treating a neurodegenerative disease in a subject are provided. In certain embodiments, the invention provides a method for treating a neurodegenerative disease in a subject, the method comprising administering to a subject in need thereof an effective amount of a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl;
R4 is H, OH, OAc, or absent;
R5 and R8 are independently H or OAc;
R6 and R7 are H or R6 and R7, taken together, are O; and
the dashed line denotes a bond when R4 is absent, or absence of a bond when R4 is H, OH, or OAc.
In other aspects, the invention provides pharmaceutical compositions that include a compound of the invention and a pharmaceutically acceptable carrier, the use of a compound of the invention as an immunosuppressive agent, and the use of a compound of the invention as a neuroprotective agent.
The foregoing aspects and many of the attendant advantages of this invention will become more readily appreciated as the same become better understood by reference to the following detailed description, when taken in conjunction with the accompanying drawings.
The present invention relates to gracilin A and its congeners and methods for using gracilin A and its congeners as immunosuppressive and neuroprotective agents.
In one aspect, the invention provides gracilin A congeners. As used herein, the term “gracilin A congener” refers to chemical substances related to gracilin A by origin, structure, or function. The terms “gracilin A congener” and “gracilin A derivative” are used interchangeably.
The following is a description of aspects and embodiments of the invention.
In one aspect, the invention provides gracilin A congeners.
In certain embodiments, the invention provides a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl;
R4 is H, OH, OAc, or absent;
R5 and R8 are independently H or OAc;
R6 and R7 are H or R6 and R7, taken together, are O; and
the dashed line denotes a bond or absence of a bond with the proviso that only one dashed line is a bond, and with a further proviso that both dashed lines denote absence of a bond when R4 is H, OH, or OAc.
In certain of these embodiments, the compound is
or a stereoisomer or racemate thereof.
In certain of these embodiments, the compound is
or a stereoisomer or racemate thereof.
Representative gracilin A congeners of the invention include compounds of formulae 22, 23a, 23b, 23c, 23d, 27a, 27b, A1, 29a, A1, A2, and A3:
As used herein, the term “stereoisomers” refers to molecules that have the same molecular formula and sequence of bonded atoms but differ in the three-dimensional arrangement of their atoms. Stereoisomers include enantiomers, diastereomers, and mixtures thereof, including racemic mixtures. The term “enantiomers” refers to two stereoisomers that are mirror images of each other and are non-superimposable. Diastereomers are stereoisomers not related through a reflection operation and include meso compounds, cis-trans isomers, E-Z isomers, and non-enantiomeric optical isomers.
All technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art, unless noted otherwise.
In another aspect of the invention, pharmaceutical compositions of gracilin A congeners are provided. In certain embodiments, the compositions include a pharmaceutically acceptable carrier and a gracilin A congener of the invention.
In further aspects, the present invention provides methods for using gracilin A and its congeners as immunosuppressive and neuroprotective agents.
In one further aspect, the invention provides a method for suppressing an immune response in a subject, the method comprising administering to a subject in need thereof an effective amount of a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl, and the dashed line denotes a bond or an absence of a bond, with the proviso that only one dashed line is a bond.
In certain embodiments of the method, the compound is:
or a stereoisomer or racemate thereof.
Representative compounds useful in the method include:
In certain embodiments of the method, the subject has been diagnosed as suffering from an autoimmune disease.
In certain embodiments of the method, the subject has been diagnosed as suffering from an inflammatory disease.
In certain embodiments, the compound inhibits interleukin-2 production. In certain of these embodiments, the interleukin-2 production is inhibited by greater than about 20%.
In certain embodiments, the compound binds to CypA with a KD of less than about 6 μM.
In certain embodiments, the compound reduces T-cell viability in vitro by less than about 10%.
The anti-inflammatory and immunosuppressive properties of representative gracilin A congeners of the invention are further described below.
In another further aspect, the invention provides gracilin A congeners that exhibit a neuroprotective effect. In certain embodiments, the invention provides a method for treating a neurodegenerative disease in a subject, the method comprising administering to a subject in need thereof an effective amount of a compound having the formula:
or a stereoisomer or racemate thereof, wherein:
R1, R2, and R3 are independently H or C1-C3 alkyl;
R4 is H, OH, OAc, or absent;
R5 and R8 are independently H or OAc;
R6 and R7 are H or R6 and R7, taken together, are O; and
the dashed line denotes a bond when R4 is absent, or absence of a bond when R4 is H, OH, or OAc.
In certain embodiments of the method, the compound is:
or a stereoisomer or racemate thereof.
Representative compounds useful in the method include:
In certain embodiments, the neurodegenerative disease is Alzheimer's disease.
In certain embodiments, the compound inhibits CypD with an IC50 of less than about 0.5 μM.
In certain embodiments, the compound inhibits CypA with an IC50 of less than about 0.3 μM.
In certain embodiments, the compound inhibits CypD and does not inhibit CypA.
The neuroprotective properties of representative gracilin A congeners of the invention are further described below.
The present disclosure identifies simplified gracilin A congeners that retain potency as immunosuppressive agents through high affinity for CypA with some derivatives displaying significantly higher affinity than gracilin A. In addition, disclosed herein are gracilin A congeners that demonstrate selectivity between CypA and CypD thus leading to potent neuroprotective effects for these congeners devoid of immunosuppressive effects.
The following is a description of the synthetic methodology for preparing the gracilin A congeners of the invention (see
The parent bis-acetoxy furanose 9 was readily obtained through a two-step oxidation/hydrogenation sequence to deliver the syn-substituted acetoxy furanose (±)-9a as a racemate along with the anti-diastereomer (±)-9b.
Derivatives devoid of the cyclohexyl substituent and exocyclic alkene were targeted, namely bicyclic bis-acetoxy furanose 18. This derivative was prepared from a common core 15a that would be employed for all other gracilin A derivatives. A Diels-Alder-Lactonization (DAL) organocascade employing diene 12 and acryloyl chloride 11 with tetramisole as Lewis base catalyst delivered an about 3:1 ratio of diastereomeric cycloadducts endo-15a/exo-15b on gram scale and in 94% ee. The required endo-diastereomer 15a could be isolated in 58% yield and a reduction with LiAlH4 followed by desilylation delivered the ketodiol 16. Swern oxidation led to a dialdehyde which was directly subjected to acid-promoted acetylation and epimerization gave the keto bis-acetoxy furanose 17 as a mixture of syn/anti diastereomers. A subsequent reduction with NaBH4 and dehydration with SOCl2 delivered the inseparable cyclohexenyl-fused, bis-acetoxy furanoses 18 as a mixture of syn/anti diastereomers and regioisomers.
Derivatives devoid of the exocyclic alkene but bearing the cyclohexyl substituent (
As a preliminary screen for bioactivity of initial gracilin A derivatives, the interaction with CypA of the initially synthesized, highly simplified bis-acetoxy furanose (±)-9 and derivatives 17 and 18 (as a mixture of inseparable olefin regioisomers) devoid of both the cyclohexyl substituent and exocylic alkene, was analyzed by surface plasmon resonance (SPR) employing cyclosporine A (CsA) and gracilin A as controls.
Association curves (KD) were measured at various concentrations compared to gracilin A and the highly simplified derivatives (±)-9, 17, and 18 showed no affinity for CypA (
Addition of the trimethyl cyclohexyl moiety to the bicyclic bis-acetoxy furanose core, led to gracilin A derivatives 22-23 with greater bioactivity. The initial diastereomeric mixture of tertiary alcohols 22 derived from cyclohexenyl zinc addition were inactive. However, upon dehydration to introduce the tri-substituted olefins in derivatives 23a-23d, immunosuppressive activity was initially observed with a 2-fold decrease in KD relative to gracilin A (2.53±0.40 μM) for alkene 23c bearing the 10S configuration (5.83±3.33 μM) with moderate IL-2 inhibition (about 19%). The interplay between the alkene position (Δ8,9 vs. Δ9,11) and the C10 configuration was borne out through derivatives 23a and 23d which exhibited nearly a 1000-fold increase in KD toward CypA (5.34±1.68 nM and 7.57±1.61 nM, respectively) compared to gracilin A. Inhibition of IL-2 production (about 41-46%) by alkenes 23a, 23d was not at the level of gracilin A (about 71%), however no impact on cell viability was observed for the more potent alkene 23d and ˜10% decrease in cell viability was observed for alkene 23a. The absence of the exocyclic methylidene at C8 in derivatives 23a, 23d with concomitant high affinity for CypA suggests that this moiety is not crucial for binding however may impact IL-2 inhibition. See
These data taken together suggest that the conformational preferences about the C8-C10 bond may play a pivotal role in bioactivity.
To fill gaps in the structure-activity relationship profile for immunosuppressive activity, changes in the oxidation state of the tetrahydrofuran (i.e., 24, 26) were targeted and the complexity of the cyclohexyl moiety (i.e., 27a,b) using previously accessed intermediates (
As described previously, neuroprotective agents can mediate their effects through inhibition of CypD, an isoform of CypA. Given the neuroprotective effects previously observed for gracilin A, the derivatives synthesized through application of BGR were also studied for their neuroprotective effects and their selectivity for CypD versus CypA PPiase activity was determined (
Surprisingly, the very simple diacetate 27b displayed the greatest differential CypD (IC50=0.48 μM) versus CypA (inactive) affinity while also displaying significant antioxidant activity in all five assays.
Biological Properties of Representative Gracilin a Derivatives CypA binding was analyzed by surface plasmon resonance (SPR) ((a) Alfonso, A., Pazos, M. J., Femandez-Araujo, A., Tobio, A., Alfonso, C., Vieytes, M. R. and Botana, L. M. Toxins 2014, 6, 96-107; (b) Sanchez, J. A., Alfonso, A., Leiros, M., Alonso, E., Rateb, M. E., Jaspars, M., Houssen, W. E., Ebel, R. and Botana, L. M. Cell Physiol. Biochem., 2015, 37, 779-792).
CypA modulates interleukin-2 release (IL-2) through the calcineurin pathway (Zydowsky, L. D., Etzkorn, F. A., Chang, H. Y., Ferguson, S. B., Stolz, L. A., Ho, S. I. and Walsh, C. T. Protein Sci., 1992, 1, 1092-1099). The cytotoxicity (
For neuroprotection assays, the compounds cytotoxicity was first assessed toward SH-SY5Y cells (Table 1). Compounds (−)-27a, (−)-27b, 29a, 29b, 22, 23a, 23b, 23c, and 23d led to cell death at the higher concentrations tested after 24 h. Due to the toxicity displayed by compounds 23a and 23d (IC50: 1.4 μM), these gracilin derivatives were assayed at lower concentrations (0.001, 0.01 and 0.1 μM) in the neuroprotective tests, whereas the other compounds were tested at 0.01, 0.1 and 1 μM.
Four parameters were monitored to determine the antioxidant potential: cell viability, mitochondrial membrane potential (ΔΨm), ROS release and glutathione (GSH) levels in the presence of the pro-oxidant H2O2. The known antioxidant vitamin E (VitE) at 25 μM was used as positive control.
Neuroprotection against H2O2 toxicity was evaluated by co-treating cells with 150 μM H2O2 and the compounds for 6 h. H2O2 decreased viability by 44.4±4.4% (p=0.00005). Eight gracilin A derivatives were protective at one or more concentrations. (−)-27a, (−)-27b, 29a, 22 and 23a protected cells against H2O2 insult at almost all concentrations (0.01, 0.1 and 1 μM;
Tetramethylrodamine methyl ester (TMRM) was used to evaluate the ΔΨm. After H2O2 treatment, cells exhibit a TMRM signal decrease of 24.1±3% (p=0.0032). 29a, 23a, (−)-27a, (−)-27b and 22 recovered ΔΨm compared to H2O2 control (
To assess if compounds were acting as ROS scavengers, carboxy-H2DCFDA was used to determine ROS levels. H2O2-treated cells increased ROS levels by 141.4±4.6% (p=0.00002) versus untreated cells. A decrease was observed after cotreatment with eight compounds, achieving untreated cell levels. Among them, five derivatives showed better results: (−)-27a, (−)-27b, 22, 23a and 23b diminishing ROS at two or three concentrations (
For GSH, the main non-enzymatic antioxidant in cells, neuroblastoma cotreated with H2O2 and (−)-27a, 22, (−)-27b and 29a recovered GSH levels (
Derivatives 29a, (−)-27a, (−)-27b and 22 prevent TBHP-induced opening (77.13}2.3%, p=0.00002) (
As CypD involvement in mPTP functioning is related with its PPIase activity (Connern, C. P. and Halestrap, A. P. Biochem. J., 1994, 302, 321-324), the modulation of this activity was studied. Compounds 29a, (−)-27a, (−)-27b and 22 inhibited CypD activity at low micromolar concentrations (IC50<0.5 μM) (
IC50 obtained after 24 h incubation and calculated with a log(inhibitor) vs. response fitting model of GraphPad Prism 5.0 software. Data of three independent experiments.
Anti-Inflammatory and Immunosuppressive Effects of Representative Gracilin Derivatives
Anti-inflammatory and immunosuppressive effects of selected gracilin A derivatives ((+)-23a and (−)-23d) are shown in
Neuroprotective Effects of Representative Gracilin Derivatives
Neuroprotective effects of selected gracilin A derivatives (21a, 27a, 27b, 29a, 21b, 22 and 23c) are compared in
Results are presented as mean±SEM of three replicates performed by triplicate. *p<0.05, **p<0.01, ***p<0.001. Nuclear expression of the transcription factors Nrf2 and NFκB-p65 in BV2 microglial cells are compared in
LPS control cells are compared to control cells (##p<0.01).
The following examples are provided for the purpose of illustrating, not limiting the invention.
In this example, the synthesis and characterization of representative gracilin derivatives of the invention are described.
General Procedures
All non-aqueous reactions were performed under a nitrogen atmosphere in oven-dried glassware. Dichloromethane (CH2Cl2), tetrahydrofuran (THF), diethyl ether (Et2O), acetonitrile (CH3CN) and toluene (PhMe) were dried by filtration through activated alumina (solvent purification system). Diisopropylethylamine (EtN(iPr)2) and triethylamine (Et3N) were distilled from calcium hydride prior to use. Other solvents and reagents were used as received from commercially available sources. Deuterated solvents were purchased from Cambridge Isotopes and used as received. 1H NMR spectra were measured at 500 MHz and referenced relative to residual chloroform (7.26 ppm) or benzene (7.16 ppm) and were reported in parts per million. Coupling constants (J) were reported in Hertz (Hz), with multiplicity reported following usual convention: s, singlet; d, doublet; t, triplet; q, quartet; dd, doublet of doublets; dt, doublet of triplets; dq, doublet of quartets; dq, doublet of quartets; td, triplet of doublets; tt, triplet of triplets; ddd, doublet of doublet of doublets; ddt, doublet of doublet of triplets; ddq, doublet of doublet of quartets; dddd, doublet of doublet of doublet of doublets; ddddt, doublet of doublet of doublet of doublet of triplets; ddquint, doublet of doublet of quintets; m, multiplet, br s, broad singlet. 13C NMR spectra were measured at 125 MHz and referenced relative to chloroform-d signal (77.23 ppm) or benzene (128.06 ppm) and were reported in parts per million (ppm). Flash column chromatography was performed with 60 Å Silica Gel (230-400 mesh) as stationary phase on an automated flash chromatography system (EtOAc/hexanes as eluent unless indicated otherwise). High-resolution mass spectra (ESI) were obtained through the Laboratory for Biological Mass Spectrometry (Texas A&M University). Thin Layer Chromatography (TLC) was performed using glass-backed silica gel F254 (Silicycle, 250 μm thickness). Visualization of developed plates was performed by fluorescence quenching or by treating with Seebach's staining solution. Fourier Transform Infrared (FTIR) spectra were recorded as thin films on NaCl plates. Optical rotations were recorded on a polarimeter at 589 nm employing a 25 mm cell. High Performance Liquid Chromatography (HPLC) was performed on a chromatographic system using various chiral columns (25 cm) as noted. X-ray diffraction was obtained by the X-ray Diffraction Laboratory at Texas A&M University. (S)-(−)-TM.HCl was purchased from TCI chemicals and used as received. All other chemicals were purchased from Sigma-Aldrich or Alfa Aesar and used as received without further purification.
Abbreviation List
EtN(iPr)2 N,N-Diisopropylethylamine
Et3N Triethylamine
NaBH4 Sodium borohydride
(S)-(−)-TM.HCl (S)-(−)-Tetramisole (levamisole) hydrochloride
LiAlH4 Lithium aluminum hydride
TBAF Tetrabutylammonium fluoride
Me2S Dimethyl sulfide
(COCl)2 Oxalyl chloride
DMAP 4-(Dimethylamino)pyridine
Ac2O Acetic anhydride
DIBAl-H Diisobutylaluminum hydride
DBU 1,8-Diazabicyclo(5.4.0)undec-7-ene
Preparation of Compounds (±)-9a, (±)-9b, (−)-15a, (+)-15b, 16, 17, 18, (−)-19, (±)-20a, (−)-21a, (−)-27, (−)-28, (−)-21b, (−)-22, (−)-23a, (−)-23b, (−)-23c, (−)-23d, (−)-24, (−)-26, (−)-27, (−)-28, (+)-15a, (+)-19, S3, (+)-21b, (+)-30, (+)-31a, (+)-31b, (±)-S7
cis- and trans-2,5-tetrahydrofuran-2,5-diyl diacetate ((±)-9a and (±)-9b): To a suspension of Pb(OAc)4 (2.0 g, 4.54 mmol, 1.1 equiv) in glacial AcOH (10 mL) was added furan 10 (0.30 mL, 4.13 mmol, 1.0 equiv) and the mixture was stirred at 23° C. for 20 h. AcOH was evaporated and Et2O was added to the residue. The precipitate was filtered, the filtrate was collected, evaporated and the residue was redissolved in anhydrous EtOAc (50 mL). 5% Rhodium on alumina (180 mg) was added and the hydrogenation was carried out under hydrogen atmosphere (1 atm, balloon) for 24 h. The solution was filtered through Celite, the filtrate was concentrated by rotary evaporation and purified by an automated flash chromatography system (5→50% EtOAc/hexanes) providing 721 mg (94% yield, 2 steps) of (±)-9a and (±)-9b as a clear colorless oil and as a mixture of two isomers (ratio 1.7:1 cis:trans, according to 500 MHz H-NMR). IR (thin film): 2921, 2851, 1745 cm−1; HRMS (ESI+) m/z calcd for C8H12LiO5 [M+Li]+: 195.0847, found: 195.0845. Spectral data matched that previously reported (Lee, S., Kaib, P. S. J. and List, B. J. Am. Chem. Soc., 2017, 139, 2156-2159).
(3aS,7aR)-5-((tert-butyldimethylsilyl)oxy)-3a,6,7,7a-tetrahydroisobenzofuran-1(31)-one ((−)-15a) and (3aR,7aR)-5-((tert-butyldimethylsilyl)oxy)-3a,6,7,7a-tetrahydroisobenzofuran-1(3H)-one ((+)-15b): To an oven-dried, 250-mL round-bottomed flask equipped with a magnetic stir bar was added silyloxydiene alcohol 12 (Abbasov, M. E., Hudson, B. M., Tantillo, D. J. and Romo, D. J. Am. Chem. Soc., 2014, 136, 4492-4495) (2.14 g, 10.0 mmol, 1.0 equiv), (S)-(−)-TM.HCl (2.40 g, 10.0 mmol, 1.0 equiv), 2,6-lutidine (3.5 mL, 30.0 mmol, 3.0 equiv) and anhydrous CH2Cl2 (100 mL, to make final concentration of silyloxydiene alcohol 0.1 M) at ambient temperature (23° C.). With vigorous stirring, 11 (1.05 mL, 13.0 mmol, 1.3 equiv) in CH2Cl2 (9.0 mL) was added over a period of 5 h by syringe pump addition. After stirring for an additional 13 h, the reaction mixture was filtered through a short pad of SiO2 and the filtrate was concentrated by rotary evaporation. Purification by automated flash chromatography (5→50% EtOAc/hexanes) afforded bicyclic γ-lactones (−)-15a (1.55 g, 58% yield, 94% ee) and (+)-15b (0.51 g, 19% yield, 94% ee).
(−)-15a: white crystalline solid; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.45; [α]D20.1=−32.77 (c=0.82, CHCl3); Enantiomeric excess was determined by chiral HPLC analysis in comparison with authentic racemic material using a Chiralcel OD-H column: hexanes:iPrOH=95:05, flow rate 0.5 mL/min, λ=210 nm: tmajor=13.5 min, tminor=15.2 min, 94% ee (Figure S1); 1H NMR (500 MHz, CDCl3) δ 4.76-4.73 (m, 1H), 4.32 (dd, J=8.8, 5.9 Hz, 1H), 3.98 (dd, J=8.8, 2.0 Hz, 1H), 3.17-3.11 (m, 1H), 2.74 (dt, J=7.8, 4.3 Hz, 1H), 2.19-2.12 (m, 1H), 2.12-2.06 (m, 1H), 1.96-1.89 (m, 1H), 1.86-1.78 (m, 1H), 0.89 (s, 9H), 0.11 (s, 3H), 0.10 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 178.48, 153.66, 103.01, 73.13, 37.65, 35.50, 26.12, 25.69 (3), 20.58, 18.10, −4.26, −4.44; IR (thin film): 2929, 2857, 1754, 1660 cm−1; HRMS (ESI+) m/z calcd for C14H24NaO3Si [M+Na]+: 291.1392, found: 291.1387.
(+)-15b: clear colorless oil; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.65; [α]D19.8=+23.84 (c=1.12, CHCl3); 1H NMR (500 MHz, CDCl3) δ 4.91 (d, J=1.3 Hz, 1H), 4.38 (dd, J=8.0, 6.6 Hz, 1H), 3.81 (dd, J=11.4, 8.0 Hz, 1H), 2.91-2.82 (m, 1H), 2.28-2.17 (m, 3H), 1.70-1.58 (m, 2H), 0.91 (s, 9H), 0.14 (s, 3H), 0.08 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 176.35, 154.19, 100.93, 71.74, 43.76, 41.00, 30.47, 25.68 (3), 20.87, 18.11, −3.46, −4.39. IR (thin film): 2930, 2857, 1777, 1640 cm−1; HRMS (ESI+) m/z calcd for C14H24NaO3Si [M+Na]+: 291.1392, found: 291.1394.
(3S,4R)-3,4-bis(hydroxymethyl)cyclohexan-1-one (16): To a 50 mL, single-necked, oven-dried, pear flask was added (−)-15a (493 mg, 1.85 mmol, 1.0 equiv) and 13.0 mL of THF. The resulting solution was cooled to 0° C. with an ice bath and stirred for 10 min before adding LiAlH4 as a solution in THF (2.4 M, 1.68 mL, 4.04 mmol, 2.2 equiv) dropwise via plastic syringe. After addition was complete, the mixture was allowed to stir for 15 min at 0° C. before quenching by the method of Fieser (Fieser, L. F.; Fieser, M. Reagents for Organic Synthesis, 1967, 581-595). After quenching at 0° C. the reaction mixture is allowed to warm to ambient temperature (23° C.) with vigorous stirring over 3 h. MgSO4 is then added to the mixture and the resulting slurry is filtered through Celite and the solvent removed by rotary evaporation, yielding an off-white oil which is used in the next step without purification.
To a 50 mL, single-necked, pear flask charged with the crude diol was added 5.0 mL of THF. The resulting solution was cooled to 0° C. with an ice bath and AcOH was added (0.53 mL, 9.20 mmol, 5.0 equiv) followed by TBAF as a solution in THF (1.0 M, 9.20 mL, 9.20 mmol, 5.0 equiv). The reaction mixture was then stirred for 1 h at 0° C. before removing the ice bath and allowing the mixture to warm to ambient temperature (23° C.) over 1 h. The reaction mixture was then concentrated by rotary evaporation and the resulting crude oil was purified by automated flash column chromatography (0→20% MeOH/EtOAc) to afford ketone diol 16 (220 mg, 76% yield, 2 steps) as a clear oil: TLC, Rf=0.51 (MeOH/EtOAc, 1:4 v/v).
(1S,3R,3aS,7aR)-5-oxooctahydroisobenzofuran-1,3-diyl diacetate (17): To a 50 mL, single-necked, oven-dried, pear flask was added 10 mL of CH2Cl2 which was cooled to −78° C. and stirred for 10 min before adding (COCl)2 (596 μL, 6.95 mmol, 5.0 equiv). The solution was then stirred for another 10 min before adding DMSO as a solution in anhydrous CH2Cl2 (2.8 M, 5.0 mL, 13.9 mmol, 10.0 equiv) slowly dropwise via plastic syringe. The resulting solution was stirred for 40 min at −78° C. Then diol 16 was added as a solution in CH2Cl2 (0.15 M, 220 mg, 1.39 mmol, 1.0 equiv) slowly dropwise via plastic syringe and stirred for 1 h. Et3N was then added to the reaction mixture (3.87 mL, 27.8 mmol, 20.0 equiv) slowly dropwise via plastic syringe. The reaction mixture was then stirred for 30 min at −78° C. before replacing cooling bath with a 0° C. ice bath and stirring for 1 h. The reaction mixture was quenched with saturated, aqueous NaHCO3, extracted with CH2Cl2 (3×15 mL), dried over MgSO4, filtered through Celite, and concentrated by rotary evaporation to give a crude oil which was used in the next step without purification.
To a 50 mL, single-necked, pear flask charged with crude material was added 7.0 mL of AcOH and 7.0 mL of Ac2O, followed by NaOAc (1.14 g, 13.9 mmol, 10.0 equiv) and concentrated H2SO4 (74 μL, 1.39 mmol, 1.0 equiv). The reaction mixture was stirred at ambient temperature (23° C.) for 48 h, poured into saturated aqueous NaHCO3 solution (50 mL), and the aqueous layer was extracted with EtOAc (3×50 mL). The combined organic layer was washed with brine (20 mL), dried over MgSO4, filtered through celite, and concentrated under reduced pressure to give a crude oil which was purified by automated flash column chromatography (0→100% EtOAc/hexanes) to afford bis-acetoxy furanose 17 (105 mg, 30% over 2 steps) as a mixture of two diastereomers with the appearance of an amber oil: TLC, Rf=0.39 (EtOAc/hexanes, 1:1 v/v).
(3aR,7aS)-1,3,3a,4,5,7a-hexahydroisobenzofuran-1,3-diyl diacetate (18): To a 50 mL, single-necked, oven-dried, pear flask charged with 17 (105 mg, 0.41 mmol, 1.0 equiv) was added 12.0 mL of THF. The resulting solution was cooled to 0° C. and stirred for 10 min before adding NaBH4 (18 mg, 0.49 mmol, 1.2 equiv) in one portion. The resulting mixture was allowed to stir for 1 h at 0° C. before quenching with AcOH (50 μL, 0.87 mmol, 2.1 equiv) and warming to ambient temperature (23° C.). The mixture was concentrated by rotary evaporation to give a crude pale yellow oil which was used in the next step without purification.
To a 10 mL, single-necked, oven-dried, pear flask charged with crude material was added 3.0 mL of pyridine. The resulting solution was cooled to 0° C. and stirred for 10 min before adding SOCl2 (87 μL, 1.20 mmol, 10.0 equiv). The cooling bath was removed and the mixture was allowed to warm to ambient temperature (23° C.) and stir for 3 h. The reaction mixture was then concentrated by high-vacuum rotary evaporation and the crude residue was purified by flash column chromatography (0→100% EtOAc/hexanes) to afford alkene bicycle 18 (16 mg, 17% yield over 2 steps) as a clear oil and a mixture of regioisomers and diastereomers as indicated in the scheme above. Rf=0.39 (EtOAc/hexanes, 1:1 v/v).
(3aS,7aR)-tetrahydroisobenzofuran-1,5(3H,4H)-dione ((−)-19): To a pre-cooled solution (0° C.) of silylenol ether (−)-15a (495 mg, 1.84 mmol, 1.0 equiv) and glacial AcOH (0.22 mL, 3.69 mmol, 2.0 equiv) in anhydrous THF (18.0 mL) was added TBAF (1.0 M in THF, 3.7 mL, 3.69 mmol, 2.0 equiv) dropwise. The reaction mixture was allowed to warm slowly up to ambient temperature (23° C.) over 4 h, and then concentrated by rotary evaporation. Purification by automated flash chromatography (5→80% EtOAc/hexanes) afforded ketolactone (−)-19 (278 mg, 98% yield).
(−)-19: clear colorless oil; TLC (EtOAc/hexanes, 1:1 v/v), Rf=0.23; [α]D20.0=−57.63 (c=0.38. CHCl3); 1H NMR (500 MHz, CDCl3) δ 4.39 (dd, J=9.4, 6.1 Hz, 1H), 3.98 (dd, J=9.4, 2.3 Hz, 1H), 3.08-2.98 (m, 1H), 2.94-2.86 (m, 1H), 2.53 (dd, J=15.2, 6.4 Hz, 1H), 2.38-2.26 (m, 4H), 2.21-2.10 (m, 1H); 13C NMR (126 MHz, CDCl3) δ 209.03, 177.64, 71.67, 41.21, 38.14, 37.34, 35.72, 22.72; IR (thin film): 2934, 2864, 1771, 1712 cm−1; HRMS (ESI+) m/z calcd for C8H11O3 [M+H]+: 155.0708, found: 155.0711.
3-Chloro-1-methylcyclohex-1-ene ((±)-20a): N-chlorosuccinimide (2.48 g, 18.6 mmol, 1.1 equiv) was weighed into an oven-dried, round-bottomed flask and suspended in CH2Cl2 (33.0 mL). The slurry was cooled to 0° C. and Me2S (1.61 mL, 21.9 mmol, 1.3 equiv) was added dropwise over −5 min producing a white solution. 3-methylcyclohex-2-en-1-ol ((±)-S1) (2.0 mL, 16.8 mmol, 1.0 equiv) was added dropwise with vigorous stirring to provide a clear solution. Within −10 min, a precipitate formed. The reaction was continued at 0° C. for 2 h and concentrated under reduced pressure. Pentane (50 mL) was added leading to immediate formation a white precipitate. The flask was then placed in a freezer for 4 h and the supernatant was decanted. The remaining solid was washed with cold pentane (2×50 mL) and the combined organics were washed with brine (2×50 mL), dried over MgSO4, and concentrated in vacuo. Allyl chloride (±)-20a was isolated as a clear colorless oil, (1.76 g, 80%) and this material was of sufficient purity (>95% as judged by 1H-NMR) to be carried directly to the next step without purification. TLC (EtOAc/hexanes, 3:7 v/v), Rf=0.79. Spectral data matched that previously reported (Ren, H., Dunet, G., Mayer, P. and Knochel, P. J. Am. Chem. Soc., 2007, 127, 5376-5377).
(3aS,5R,7aR)-5-hydroxy-5-(1-methylcyclohexyl)hexahydroisobenzofuran-1(31)-one ((−)-21a): To an oven-dried round-bottomed flask was added zinc powder (2.3 g, 35.0 mmol, 25 equiv) and LiCl (300 mg, 7.0 mmol, 5.0 equiv) with a large bore vacuum adapter with Teflon lined seal for addition of liquids via syringe. The flask and solids were then carefully flame-dried under high vacuum (using a vacuum/N2 double manifold) by repeated heating and cooling cycles with swirling of the solids to avoid the solids from clumping. After the final cycle of heating, the solids were allowed to cool for 3 min under N2, and then anhydrous THF (20.0 mL) was slowly and carefully added with vigorous stirring. After addition of THF, 1,2-dibromoethane (0.12 mL, 1.4 mmol, 1.0 equiv) was added which sometimes led to an exotherm simultaneous with rapid gas evolution, which needs to be controlled. Pressure fluctuations generated upon addition of 1,2-dibromethane were controlled by momentarily switching the manifold from N2 to vacuum. [Note: If gas evolution was not observed upon addition of 1,2-dibromoethane, a heat gun was employed to initiate gas evolution]. The solution was allowed to cool at ambient temperature over ˜5 min before being further cooled to 0° C. A solution of (−)-19 (215 mg, 1.4 mmol, 1.0 equiv) in THF (1.0 mL) was added dropwise by syringe followed by slow addition of (±)-20a (1.65 g, 12.6 mmol, 9.0 equiv) in THF (4.0 mL) via syringe pump at a rate of 1.0 mL/h. During this time, the reaction mixture was allowed to slowly warm to ambient temperature (23° C.). The reaction mixture was then quenched with saturated NH4Cl solution (100 mL). The organic phase was separated and the aqueous phase was further extracted with EtOAc (3×100 mL). The organic layers were combined, washed with brine (200 mL), dried with MgSO4, and concentrated in vacuo to afford cyclohexenol lactone S2 as a crude pale yellow oil and as an inseparable mixture of two diastereomers (1:1 dr, based on 1H-NMR analysis of the crude mixture) which was used in the next step without purification.
To a solution of the above crude cyclohexenol lactone S2 (267 mg, 1.07 mmol, 1.0 equiv) in absolute EtOH (25.0 mL) and under an atmosphere of N2 was added Pd(OH)2 on carbon (300 mg, 20 wt %, 2.14 mmol, 2.0 equiv). A H2 balloon was attached to the flask and after three consecutive cycles of evacuation (under high vacuum) and H2 purge, the mixture was stirred for 15 h. An aliquot was removed for H-NMR analysis to ensure complete hydrogenation of the cyclohexene moiety. The reaction mixture was filtered through a pad of Celite, washed with EtOAc and concentrated in vacuo. Purification by automated flash chromatography (5→60% EtOAc/hexanes) afforded cyclohexanol lactone (−)-21a (307 mg, 88% yield over 2 steps) as a single diastereomer (>19:1 dr, based on 1H-NMR analysis of the crude mixture).
(−)-21a: colorless crystalline solid; TLC (EtOAc/hexanes, 1:1 v/v), Rf=0.43; [α]D17.0=−34.15 (c=0.41, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; 1H NMR (500 MHz, CDCl3) δ 4.23 (ddd, J=8.8, 4.7, 0.7 Hz, 1H), 3.91 (d, J=8.9 Hz, 1H), 2.75 (dq, J=11.9, 6.2 Hz, 1H), 2.63 (t, J=6.4 Hz, 1H), 2.10-1.92 (m, 2H), 1.84 (ddd, J=13.8, 6.0, 2.9 Hz, 1H), 1.66-1.50 (m, 4H), 1.45-1.19 (m, 9H), 1.07-0.95 (m, 1H), 0.88 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 178.65, 75.45, 72.07, 40.06, 38.94, 33.21, 31.34, 30.43, 30.35, 26.36, 26.27, 22.03 (2), 18.65, 17.22; IR (thin film): 3509, 2966, 2929, 2862, 1747 cm−1; HRMS (ESI+) m/z calcd for C15H25O3 [M+H]+: 253.1804, found: 253.1793.
(3aS,7aR)-5-(1-methylcyclohexyl)-3a,6,7,7a-tetrahydroisobenzofuran-1(3H)-one ((−)-26): A glass tube was charged with a solution of (−)-21a (268 mg, 1.07 mmol, 1.0 equiv) in anhydrous CHCl3 (35.0 mL) and Martin Sulfurane dehydrating agent (1.44 g, 2.14 mmol, 2.0 equiv). The tube was sealed and placed in a 60° C. oil bath for 1 h. The reaction mixture was cooled to ambient temperature (23° C.), concentrated under reduced pressure and purified by automated flash chromatography (5→30% EtOAc/hexanes) to afford alkene lactone (−)-26 (182 mg, 73% yield) as a single regioisomer (>19:1 rr, based on 1H-NMR analysis of the crude mixture).
(−)-26: clear colorless oil; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.40; [α]D16.9=−72.31 (c=0.26, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; 1H NMR (500 MHz, CDCl3) δ 5.38 (s, 1H), 4.38 (dd, J=8.8, 6.7 Hz, 1H), 4.02 (dd, J=8.8, 3.0 Hz, 1H), 3.10 (dtq, J=8.4, 5.3, 3.1 Hz, 1H), 2.76 (dt, J=8.0, 4.9 Hz, 1H), 2.08 (dt, J=13.3, 4.8 Hz, 1H), 2.02-1.88 (m, 2H), 1.73 (dddd, J=13.1, 10.0, 5.4, 4.7 Hz, 1H), 1.69-1.58 (m, 2H), 1.48-1.17 (m, 8H), 0.92 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 179.14, 147.95, 117.80, 72.89, 38.86, 37.91, 37.85, 36.08, 35.97, 27.03, 26.43, 22.73, 22.52, 21.35, 20.81; IR (thin film): 2927, 2854, 1771 cm−1; HRMS (ESI+) m/z calcd for C15H22LiO2 [M+Li]+: 241.1780, found: 241.1793.
(1S,3aS,7aR)-5-(1-methylcyclohexyl)-1,3,3a,6,7,7a-hexahydroisobenzofuran-1-yl acetate ((−)-27): To a solution of (−)-26 (30 mg, 0.128 mmol, 1.0 equiv) in CH2Cl2 (2.0 mL) was added DIBAl-H (1.0 M solution in CH2Cl2, 154 μL, 0.154 mmol, 1.2 equiv) dropwise at −78° C. The reaction mixture was stirred at −78° C. for 4 h and carefully quenched in sequence with 6 μL H2O, 11 μL 15% aqueous NaOH, and 16 μL H2O. The dry-ice bath was removed and the mixture was allowed slowly warm to ambient temperature (23° C.). Subsequently, anhydrous MgSO4 was added and the reaction mixture was vigorously stirred for 30 min, filtered through a pad of Celite and concentrated by rotary evaporation. The crude lactol was of sufficient purity (>95% as judged by 1H-NMR) to be carried on directly to the next step.
To a solution of the above crude lactol in anhydrous pyridine (1.0 mL) was added DMAP (2.0 mg, 0.012 mmol, 0.1 equiv) and Ac2O (120 μL, 1.28 mmol, 10.0 equiv). The reaction mixture was stirred at ambient temperature (23° C.) for 14 h, poured into saturated aqueous NaHCO3 solution (10 mL), and the aqueous layer was extracted with CH2Cl2 (3×10 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (0→30% EtOAc/hexanes) afforded acetyl lactol (−)-27 (21 mg, 60% yield over 2 steps) as a single diastereomer (>19:1 dr, based on H-NMR analysis of the crude mixture).
(−)-27: clear colorless oil; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.49; [α]D19.1=−53.33 (c=0.15, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; 1H NMR (500 MHz, CDCl3) δ 6.04 (s, 1H), 5.44 (dd, J=4.3, 1.6 Hz, 1H), 4.28 (t, J=8.2 Hz, 1H), 3.65 (t, J=7.8 Hz, 1H), 3.00 (q, J=7.5 Hz, 1H), 2.30 (ddd, J=11.6, 7.3, 4.4 Hz, 1H), 2.13-2.08 (m, 1H), 2.07 (s, 3H), 1.96-1.87 (m, 1H), 1.84 (dq, J=13.5, 4.5 Hz, 1H), 1.71-1.62 (m, 3H), 1.52-1.22 (m, 8H), 0.95 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.77, 145.87, 117.56, 103.91, 74.92, 43.14, 38.68, 36.56, 36.26, 36.03, 27.10, 26.54, 22.86, 22.79, 22.69, 22.54, 21.53; IR (thin film): 2931, 2857, 1746 cm−1; HRMS (ESI+) m/z calcd for C17H26LiO3 [M+Li]+: 285.2042, found: 285.2052.
(3aS,5R,7aR)-5-hydroxy-5-(1,5,5-trimethylcyclohex-2-en-1-yl)hexahydroisobenzo-furan-1(3H)-one ((−)-21b): To an oven-dried round-bottomed flask was added zinc powder (2.3 g, 35.0 mmol, 25 equiv) and LiCl (300 mg, 7.0 mmol, 5.0 equiv) with a large bore vacuum adapter with Teflon lined seal for addition of liquids via syringe. The flask and solids were then carefully flame-dried under high vacuum (using a vacuum/N2 double manifold) by repeated heating and cooling cycles with swirling of the solids to avoid the solids from clumping. After the final cycle of heating, the solids were allowed to cool for 3 min under N2, and then anhydrous THF (20.0 mL) was slowly and carefully added with vigorous stirring. After addition of THF, 1,2-dibromoethane (0.12 mL, 1.4 mmol, 1.0 equiv) was added which sometimes led to an exotherm simultaneous with rapid gas evolution, which needs to be controlled. Pressure fluctuations generated upon addition of 1,2-dibromoethane were controlled by momentarily switching the manifold from N2 to vacuum. [Note: If gas evolution was not observed upon addition of 1,2-dibromoethane, a heat gun was employed to initiate gas evolution]. The solution was allowed to cool at ambient temperature over ˜5 min before being further cooled to 0° C. A solution of keto lactone (−)-19 (215 mg, 1.4 mmol, 1.0 equiv) in THF (1.0 mL) was added dropwise by syringe followed by slow addition of allyl chloride (±)-20b (Harvey, N. L., Krysiak, J., Chamni, S., Cho, S. W., Sieber, S. A. and Romo, D. Chem. Eur. J., 2015, 21, 1425-1428) (2.22 g, 14.0 mmol, 10 equiv) in THF (4.0 mL) via syringe pump at a rate of 1.0 mL/h. During this time, the reaction mixture was allowed to slowly warm to ambient temperature (23° C.). The reaction mixture was then quenched with saturated NH4Cl solution (100 mL). The organic phase was separated and the aqueous phase was further extracted with EtOAc (3×100 mL). The organic layers were combined, washed with brine (200 mL), dried with MgSO4, and concentrated under reduced pressure. Purification by automated flash chromatography (5→60% EtOAc/hexanes) afforded dimethyl cyclohexenol lactone (−)-21b (318 mg, 82% yield) as an inseparable mixture of two diastereomers (1:1 dr, based on 1H-NMR analysis of the crude mixture).
(−)-21b: white crystalline solid, m.p. 161.2-163.7° C. (recrystallized from pentane); TLC (EtOAc/hexanes, 1:1 v/v), Rf=0.44; [α]D17.3=−28.89 (c=0.18, CHCl3); Absolute stereochemistry was assigned based on X-ray analysis using anomalous dispersion. 1H NMR (500 MHz, CDCl3) δ 5.89 (ddd, J=9.9, 6.3, 2.1 Hz, 1H), 5.52 (dd, J=10.2, 1.8 Hz, 1H), 4.24 (dt, J=9.0, 4.5 Hz, 1H), 3.93 (dd, J=8.9, 4.3 Hz, 1H), 2.75 (tt, J=12.0, 6.1 Hz, 1H), 2.64 (q, J=5.5 Hz, 1H), 2.14-2.02 (m, 1H), 2.05-1.92 (m, 1H), 1.86-1.64 (m, 4H), 1.63-1.50 (m, 1H), 1.45-1.21 (m, 3H), 1.10 (dtd, J=13.6, 2.7, 1.3 Hz, 1H), 1.02 (s, 3H), 0.97 (s, 3H), 0.94 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 178.63, 130.27 (2), 75.44, 71.97, 43.43, 42.90, 39.05, 38.26, 33.22, 32.34, 30.92, 30.59, 27.20, 25.98, 22.16, 18.69; IR (thin film): 3518, 2951, 2867, 1766 cm−1; HRMS (ESI+) m/z calcd for C17H27O3 [M+H]*: 279.1960, found: 279.1963.
(1S,3R,3aS,5R,7aR)-5-hydroxy-5-(1,3,3-trimethylcyclohexyl)octahydroisobenzo-furan-1,3-diyl diacetate ((−)-22): To a solution of cyclohexenol lactone (−)-21b (281 mg, 1.01 mmol, 1.0 equiv) in absolute EtOH (20.0 mL) and under an atmosphere of N2 was added Pd(OH)2 on carbon (284 mg, 20 wt %, 2.02 mmol, 2.0 equiv). A H2 balloon was attached to the flask and after three consecutive cycles of evacuation (under high vacuum) and H2 purge, the mixture was stirred for 18 h. An aliquot was removed for 1H-NMR analysis to ensure complete hydrogenation of the cyclohexene moiety. The reaction mixture was filtered through a pad of Celite, washed with EtOAc, concentrated in vacuo, and taken on directly to the next step without purification.
To a solution of the above crude lactone in anhydrous THF (9.0 mL) was added LiAlH4 (2.0 M solution in THF, 0.60 mL, 1.2 mmol, 2.7 equiv) dropwise at 0° C. After stirring for 20 min, the ice bath was removed and the mixture was allowed to slowly warm to ambient temperature (23° C.) over 40 min. Upon consumption of the starting material (as judged by TLC), the reaction mixture was cooled to 0° C. and carefully quenched in sequence with 50 μL H2O, 85 μL 15% aqueous NaOH, and 120 μL H2O. The ice bath was removed and the mixture was allowed to slowly warm to ambient temperature (23° C.). Subsequently, anhydrous MgSO4 was added and the reaction mixture was vigorously stirred for 30 min, filtered through a pad of Celite and concentrated by rotary evaporation. The crude diol was of sufficient purity (>95% as judged by 1H-NMR) to be carried on directly to the next step.
To a solution of (COCl)2 (0.19 mL, 2.2 mmol, 10.0 equiv) in CH2Cl2 (15.0 mL) was added DMSO (0.31 mL, 4.4 mmol, 20.0 equiv) followed by a solution of the above crude diol in CH2Cl2 (5.0 mL) via syringe pump over 1 h at −78° C. To the reaction mixture was added Et3N (0.92 mL, 6.6 mmol, 30.0 equiv) and the reaction mixture was stirred at −78° C. for 1 h, and then at ambient temperature (23° C.) for 1 h. The mixture was quenched with 2 N HCl (10 mL) and the aqueous layer was extracted with CH2Cl2 (3×50 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. The crude dialdehyde was taken on directly to the next step without purification.
To a solution of the above crude dialdehyde in AcOH/Ac2O (1.5:1, 4.5/3.0 mL) was added NaOAc (180 mg, 2.2 mmol, 10.0 equiv) and concentrated H2SO4 (11 μL, 0.22 mmol, 1.0 equiv) successively at 0° C. The reaction mixture was stirred at ambient temperature (23° C.) for 48 h, poured into saturated aqueous NaHCO3 solution (10 mL), and the aqueous layer was extracted with EtOAc (3×50 mL). The combined organic layer was washed with brine (20 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (0→20% EtOAc/hexanes) afforded cyclohaxanol diacetate (−)-22 (142 mg, 37% yield over 4 steps) as an inseparable mixture of two diastereomers (1:1 dr, based on 1H-NMR analysis of the crude mixture).
(−)-22: white solid; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.10; [α]D19.1=−10.53 (c=0.19, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; 1H NMR (500 MHz, CDCl3) δ 6.17 (d, J=6.6 Hz, 1H), 5.94 (d, J=2.1 Hz, 1H), 2.65-2.57 (m, 1H), 2.59-2.49 (m, 1H), 2.12 (s, 3H), 2.08 (s, 3H), 2.02-1.77 (m, 2H), 1.70-1.47 (m, 5H), 1.42-1.21 (m, 7H), 1.15-1.09 (m, 1H), 1.01 (s, 3H), 1.00 (s, 3H), 0.91 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.93, 170.12, 103.22, 100.78, 75.72, 43.29, 41.48, 40.91, 39.15, 38.92, 36.16, 30.79 (2), 30.10, 28.82, 27.80, 25.45, 21.42, 20.51, 18.98, 18.54; IR (thin film): 3527, 2948, 2865, 1748 cm−1; HRMS (ESI−) m/z calcd for C21H34ClO6 [M+Cl]−: 417.2044, found: 417.2062.
(1S,3R,3aS,7aR)-5-((S)-1,3,3-trimethylcyclohexyl)-1,3,3a,4,7,7a-hexahydroisoben-zofuran-1,3-diyl diacetate ((−)-23a), (1S,3R,3aS,7aR)-5-((R)-1,3,3-trimethylcyclo-hexyl)-1,3,3a,4,7,7a-hexahydroisobenzofuran-1,3-diyl diacetate ((−)-23b), (1R,3S,3aR,7aS)-6-((S)-1,3,3-trimethylcyclohexyl)-1,3,3a,4,5,7a-hexahydroisobenzofuran-1,3-diyl diacetate ((−)-23c), and (0R,3S,3aR,7aS)-6-((R)-1,3,3-trimethyl-cyclohexyl)-1,3,3a,4,5,7a-hexahydroisobenzofuran-1,3-diyl diacetate ((−)-23)): A glass tube was charged with cyclohaxanol diacetate (−)-22 (20 mg, 0.052 mmol, 1.0 equiv) and methyl N-(triethylammoniosulfonyl)carbamate (Burgess reagent, 25 mg, 0.10 mmol, 2.0 equiv) in PhH (2.0 mL). The tube was sealed and placed in an 80° C. oil bath for 1 h. The reaction mixture was cooled to ambient temperature (23° C.) and concentrated under reduced pressure. Purification by automated flash chromatography (0→20% EtOAc/hexanes) afforded cyclohexenyl diacetate (−)-23 (18 mg, 97% yield) as a clear colorless oil and as an inseparable mixture of four diastereomers (1:1 dr, 1.3:1 rr, based on 1H-NMR analysis of the crude mixture). TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.32; [α]D20.2=−280.1 (c=0.011, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; IR (thin film): 2924, 2864, 1750 cm−1; HRMS (ESI+) m/z calcd for C21H32NaO5 [M+Na]+: 387.2147, found: 387.2151. This diastereomeric mixture was then subjected to a chiral HPLC purification using a Chiralcel OJ-H column: hexanes:iPrOH=96:04, flow rate 0.4 mL/min, λ=210 nm: t(−)-23a=11.4-13.5 min, t(−)-23b=13.6-15.4 min, t(−)-23e=15.5-17.5 min, t(−)-23d=17.6-20.8 min.
(−)-23a: 1H NMR (500 MHz, CDCl3) δ 6.11 (d, J=2.1 Hz, 1H), 6.03 (d, J=3.0 Hz, 1H), 5.50 (d, J=4.1 Hz, 1H), 2.96-2.92 (m, 1H), 2.52 (dddd, J=9.4, 7.3, 4.5, 2.2 Hz, 1H), 2.11 (s, 3H), 2.09 (s, 3H), 1.98-1.88 (m, 2H), 1.81-1.70 (m, 1H), 1.67-1.56 (m, 3H), 1.49-1.38 (m, 1H), 1.33-1.23 (m, 2H), 1.18-1.08 (m, 2H), 1.06-0.99 (m, 1H), 0.91 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.47, 170.33, 147.93, 114.39, 103.69, 103.10, 48.24, 44.10, 41.73, 40.41, 39.02, 36.32, 33.30, 31.55, 31.46, 26.71, 22.64, 21.79, 21.52, 21.49, 19.59.
(−)-23b: 1H NMR (500 MHz, CDCl3) δ 6.09 (d, J=1.5 Hz, 1H), 6.00 (d, J=3.6 Hz, 1H), 5.50 (d, J=4.3 Hz, 1H), 3.01-2.94 (m, 1H), 2.47 (dddd, J=9.7, 7.5, 4.6, 1.6 Hz, 1H), 2.11 (s, 3H), 2.09 (s, 3H), 2.00-1.90 (m, 2H), 1.86-1.80 (m, 1H), 1.66-1.52 (m, 2H), 1.48-1.36 (m, 2H), 1.33-1.23 (m, 2H), 1.19-1.08 (m, 2H), 1.04-0.98 (m, 1H), 0.91 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.37, 170.21, 147.82, 114.13, 104.02, 103.07, 47.67, 43.43, 41.99, 40.34, 38.88, 36.06, 35.10, 33.15, 31.40, 26.78, 24.62, 23.01, 22.53, 21.36, 19.38.
(−)-23c: 1H NMR (500 MHz, CDCl3) δ 6.01 (d, J=2.5 Hz, 1H), 5.97 (d, J=2.7 Hz, 1H), 5.57 (t, J=4.4 Hz, 1H), 2.65-2.53 (m, 2H), 2.36-2.22 (m, 2H), 2.10 (s, 3H), 2.09 (s, 3H), 2.07-1.95 (m, 2H), 1.93-1.87 (m, 1H), 1.63-1.53 (m, 2H), 1.48-1.38 (m, 20 1H), 1.31-1.23 (m, 1H), 1.18-1.10 (m, 2H), 1.03 (d, J=14.0 Hz, 1H), 0.92 (s, 3H), 0.86 (s, 3H), 0.79 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.41, 170.34, 143.99, 116.76, 104.27, 104.05, 48.17, 42.11, 40.65, 40.35, 38.53, 36.19, 33.20, 31.40, 31.28, 27.10, 24.35, 23.66, 21.49 (2), 19.54.
(−)-23d: 1H NMR (500 MHz, CDCl3) δ 6.06 (d, J=1.8 Hz, 1H), 6.01 (d, J=3.1 Hz, 1H), 5.57 (dt, J=5.5, 2.8 Hz, 1H), 2.50 (ddt, J=10.2, 7.3, 3.1 Hz, 1H), 2.46-2.35 (m, 3H), 2.09 (s, 3H), 2.09 (s, 3H), 2.00-1.76 (m, 3H), 1.57 (d, J=13.7 Hz, 1H), 1.49-1.38 (m, 1H), 1.31-1.22 (m, 2H), 1.14 (ddd, J=13.8, 11.5, 3.6 Hz, 2H), 1.03 (d, J=13.9 Hz, 1H), 0.91 (s, 3H), 0.86 (s, 3H), 0.78 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.38, 170.30, 144.90, 116.91, 104.09, 103.66, 48.27, 43.18, 41.11, 40.27, 38.67, 36.07, 33.01, 31.40, 30.94, 27.17, 24.58, 24.52, 21.49, 21.47, 19.53.
(1S,3aS,7aR)-5-oxooctahydroisobenzofuran-1-yl acetate ((−)-24): To a solution of lactone (−)-15a (72 mg, 0.268 mmol, 1.0 equiv) in CH2Cl2 (2.7 mL) was added DIBAl-H (1.0 M solution in CH2Cl2, 321 μL, 0.321 mmol, 1.2 equiv) dropwise at −78° C. The reaction mixture was stirred at −78° C. for 4 h and carefully quenched in sequence with 12 μL of H2O, 12 μL of 20% aqueous NaOH, and 32 μL of H2O. The dry-ice bath was removed and the mixture was allowed to slowly warm to ambient temperature (23° C.). Subsequently, anhydrous MgSO4 was added and the reaction mixture was vigorously stirred for 30 min, filtered through a pad of Celite and concentrated under reduced pressure. The crude silyl enol ether lactol was of sufficient purity (>95% as judged by 1H-NMR) to be carried on directly to the next step.
To a pre-cooled solution (0° C.) of the above crude silyl enol ether lactol and glacial AcOH (31 μL, 0.536 mmol, 2.0 equiv) in anhydrous THF (2.7 mL) was added TBAF (1.0 M in THF, 0.54 mL, 0.536 mmol, 2.0 equiv) dropwise. The reaction mixture was allowed to slowly warm to ambient temperature (23° C.) over 4 h, and then concentrated under reduced pressure. Purification by automated flash chromatography (5→80% EtOAc/hexanes) afforded ketolactol S3 (42 mg, 81% yield over 2 steps) as a mixture of two diastereomers (4:1 dr, based on 1H-NMR analysis of the crude mixture).
S3: clear colorless oil; TLC (EtOAc/hexanes, 4:1 v/v), Rf=0.23; (NMR data is provided for the major diastereomer) 1H NMR (500 MHz, C6D6) δ 5.08 (s, 1H), 3.94 (dd, J=8.6, 7.4 Hz, 1H), 3.27 (dd, J=8.6, 5.6 Hz, 1H), 2.40 (q, J=7.0 Hz, 1H), 2.15-1.85 (m, 5H), 1.76 (ddd, J=16.4, 11.2, 5.2 Hz, 1H), 1.50-1.38 (m, 1H), 1.36-1.23 (m, 1H); 13C NMR (126 MHz, C6D6) δ 209.93, 103.43, 71.70, 44.13, 40.38, 37.76, 36.50, 23.83; IR (thin film): 3383, 2924, 1710 cm−1; HRMS (ESI+) m/z calcd for C8H12LiO3 [M+Li]+: 163.0941, found: 163.0949.
To a solution of ketolactol S3 (20 mg, 0.128 mmol, 1.0 equiv) in anhydrous pyridine (1.0 mL) was added DMAP (2.0 mg, 0.012 mmol, 0.1 equiv) and Ac2O (120 μL, 1.28 mmol, 10.0 equiv). The reaction mixture was stirred at ambient temperature (23° C.) for 18 h, poured into saturated aqueous NaHCO3 solution (10 mL), and the aqueous layer was extracted with CH2Cl2 (3×10 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (0→40% EtOAc/hexanes) afforded acetyl ketolactol (−)-24 (24 mg, 98% yield) as a single diastereomer (>19:1 dr, based on 1H-NMR analysis of the crude mixture).
(−)-24: clear colorless oil; TLC (EtOAc/hexanes, 4:1 v/v), Rf=0.49; [α]D19.7=−21.08 (c=0.13, CHCl3); 1H NMR (500 MHz, C6D6) δ 6.05 (s, 1H), 3.76 (dd, J=8.9, 7.7 Hz, 1H), 3.23 (dd, J=8.8, 5.8 Hz, 1H), 2.19-2.09 (m, 1H), 1.96-1.83 (m, 3H), 1.70-1.60 (m, 4H), 1.46-1.33 (m, 2H), 1.26-1.10 (m, 1H); 13C NMR (126 MHz, C6D6) δ 208.12, 169.37, 103.35, 73.17, 43.34, 39.90, 37.57, 35.88, 23.42, 20.89; IR (thin film): 2957, 2923, 2853, 1712 cm−1; HRMS (ESI+) m/z calcd for C10H14LiO4 [M+Li]+: 205.1047, found: 205.1052.
(1S,3aR,7aS)-3,6-dioxooctahydroisobenzofuran-1-yl acetate ((−)-25): To a stirred solution of ketolactone (−)-19 (21 mg, 0.134 mmol, 1.0 equiv) in H2O (1.0 mL, 0.13 M) was added KOH (11 mg, 0.201 mmol, 1.5 equiv) at ambient temperature (23° C.) and stirred for 20 min. The solvent was removed under reduced pressure and azeotroped with PhMe (3×10 mL) to provide corresponding ketoacid as an amorphous solid.
To a pre-cooled solution (0° C.) of the above crude ketoacid in DMF (5.0 mL) was added Dess-Martin periodinane (292 mg, 0.688 mmol, 5.0 equiv) and NaHCO3 (224 mg, 2.67 mmol, 20 equiv). The reaction mixture was allowed to slowly warm to ambient temperature (23° C.) and stirred for 20 h. The reaction mixture was then quenched with H2O (20 mL) and extracted with EtOAc (2×20 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (5→80% EtOAc/hexanes) afforded hydroxy ketolactone S4 (5.5 mg, 24% yield over 2 steps) as an amorphous white solid and as a mixture of two diastereomers (1:1 dr, based on H-NMR analysis of the crude mixture). TLC (EtOAc/hexanes, 4:1 v/v), Rf=0.24.
To a stirred solution of hydroxyl ketolactone S4 (5.0 mg, 0.0294 mmol, 1.0 equiv) in pyridine (1.0 mL, 0.03 M) was added Ac2O (28 μL, 0.294 mmol, 10 equiv) and DMAP (3.6 mg, 0.0294 mmol, 1.0 equiv) at ambient temperature (23° C.). The reaction mixture was stirred for 4 h at 23° C. and then concentrated under reduced pressure. The crude reaction mixture was subjected to purification by automated flash column chromatography (0→100% EtOAc/hexanes) to afford acetoxy ketolactone (−)-25 (2.1 mg, 34% yield) as a single diastereomer (>19:1 dr, based on H-NMR analysis of the crude mixture).
(−)-25: white solid; TLC (EtOAc/hexanes, 4:1 v/v), Rf=0.45; [α]D20.1=−11.53 (c=0.021, CHCl3); 1H NMR (500 MHz, CDCl3) δ 6.69 (d, J=6.2 Hz, 1H), 3.33 (dtd, J=10.4, 6.6, 4.2 Hz, 1H), 3.02 (dt, J=10.0, 6.7 Hz, 1H), 2.54-2.39 (m, 4H), 2.39-2.20 (m, 2H), 2.07 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 207.85, 176.43, 168.69, 94.73, 37.51, 36.47, 31.97, 23.53, 20.71, 17.32.
(3aR,7aS)-5-((tert-butyldimethylsilyl)oxy)-3a,6,7,7a-tetrahydroisobenzofuran-1(3H)-one ((+)-15a): To a glass tube containing a solution of trans-fused bicyclic γ-lactone (+)-15b (202 mg, 0.752 mmol, 1.0 equiv) in CH2Cl2 (7.5 mL, 0.1 M) was added DBU (0.34 mL, 2.25 mmol, 3.0 equiv). The glass tube was sealed and placed in a 40° C. oil bath for 24 h. The reaction mixture was cooled to ambient temperature (23° C.), filtered through a short pad of SiO2 and the filtrate was concentrated by rotary evaporation. Purification by automated flash chromatography (5→50% EtOAc/hexanes) afforded cis-fused bicyclic γ-lactone (+)-15a (182 mg, 90% yield, 94% ee).
(+)-15a: white crystalline solid; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.45; [α]D19.9=+52.18 (c=0.77, CHCl3); Enantiomeric excess was determined by chiral HPLC analysis in comparison with authentic racemic material using a Chiralcel OD-H column: hexanes: iPrOH=95:05, flow rate 0.5 mL/min, λ=210 nm: tminor=13.5 min, tmajor=15.0 min, 94% ee; Absolute stereochemistry was assigned by analogy to bicyclic γ-lactone (+)-3c′; all spectral data matched that reported for cis-fused bicyclic γ-lactone (−)-15a.
(3aR,7aS)-tetrahydroisobenzofuran-1,5(3H,4H)-dione ((+)-19): To a pre-cooled solution (0° C.) of bicyclic γ-lactone (+)-15a (72 mg, 0.268 mmol, 1.0 equiv) and glacial AcOH (31 μL, 0.536 mmol, 2.0 equiv) in anhydrous THF (2.7 mL, 0.1 M) was added TBAF (1.0 M in THF, 0.54 mL, 0.536 mmol, 2.0 equiv) dropwise. The reaction mixture was allowed to slowly warm to ambient temperature (23° C.) over 4 h, and then concentrated under reduced pressure. Purification by automated flash chromatography (5→80% EtOAc/hexanes) afforded ketolactone (+)-19 (38 mg, 93% yield).
(+)-19: clear colorless oil; TLC (EtOAc/hexanes, 1:1 v/v), Rf=0.23; [α]D20.0=+24.35 (c=0.11. CHCl3); Absolute stereochemistry was assigned by analogy to bicyclic γ-lactone (+)-3c′; all spectral data matched that reported for ketolactone (+)-19.
(3aR,5S,7aS)-5-hydroxy-5-(1,5,5-trimethylcyclohex-2-en-1-yl)hexahydroisobenzo-furan-1(3H)-one ((+)-21b): To an oven-dried round-bottomed flask was added zinc powder (766 mg, 11.6 mmol, 25 equiv) and LiCl (100 mg, 2.33 mmol, 5.0 equiv) with a large bore vacuum adapter with Teflon lined seal for addition of liquids via syringe. The flask and solids were then carefully flame-dried under high vacuum (using a vacuum/N2 double manifold) by repeated heating and cooling cycles with swirling of the solids to avoid the solids from clumping. After the final cycle of heating, the solids were allowed to cool for 3 min under N2, and then anhydrous THF (6.7 mL) was slowly and carefully added with vigorous stirring. After addition of THF, 1,2-dibromoethane (40 μL, 0.46 mmol, 1.0 equiv) was added which sometimes led to an exotherm simultaneous with rapid gas evolution, which needs to be controlled. Pressure fluctuations generated upon addition of 1,2-dibromoethane were controlled by momentarily switching the manifold from N2 to vacuum. [Note: If gas evolution was not observed upon addition of 1,2-dibromoethane, a heat gun was employed to initiate gas evolution]. The solution was allowed to cool at ambient temperature over −5 min before being further cooled to 0° C. A solution of ketolactone (+)-19 (71 mg, 0.46 mmol, 1.0 equiv) in THF (0.33 mL) was added dropwise by syringe followed by slow addition of allyl chloride(±)-20b (740 mg, 4.6 mmol, 10 equiv) in THF (1.3 mL) via syringe pump at a rate of 1.0 mL/h. During this time, the reaction mixture was allowed slowly warm to ambient temperature (23° C.). The reaction mixture was then quenched with saturated NH4Cl solution (30 mL). The organic phase was separated and the aqueous phase was further extracted with EtOAc (3×30 mL). The organic layers were combined, washed with brine (10 mL), dried with MgSO4, and concentrated under reduced pressure. Purification by automated flash chromatography (5→60% EtOAc/hexanes) afforded dimethyl cyclohexenol lactone (+)-21b (98 mg, 78% yield) as an inseparable mixture of two diastereomers (1:1 dr, based on 1H-NMR analysis of the crude mixture).
(+)-21b: white crystalline solid; TLC (EtOAc/hexanes, 1:1 v/v), Rf=0.44; [α]D19.8=+31.44 (c=0.23, CHCl3); Absolute stereochemistry was assigned by analogy to dimethyl cyclohexenol lactone (−)-21b; All spectral data matched that reported for dimethyl cyclohexenol lactone (−)-21b.
(3aR,7aS)-5-(1,3,3-trimethylcyclohexyl)-3a,6,7,7a-tetrahydroisobenzofuran-1(3H-one ((+)-28): To a solution of dimethyl cyclohexenol lactone (+)-21b (93 mg, 0.33 mmol, 1.0 equiv) in absolute EtOH (6.6 mL) and under an atmosphere of N2 was added Pd(OH)2 on carbon (94 mg, 20 wt %, 0.67 mmol, 2.0 equiv). A H2 balloon was attached to the flask and after three consecutive cycles of evacuation (under high vacuum) and H2 purge, the mixture was stirred for 15 h. An aliquot was removed for 1H-NMR analysis to ensure complete hydrogenation of the cyclohexene moiety. The reaction mixture was filtered through a pad of Celite, washed with EtOAc, concentrated under reduced pressure, and taken on directly to the next step without purification.
A glass tube was charged with the above crude alcohol (89 mg, 0.33 mmol, 1.0 equiv) in anhydrous CHCl3 (11.6 mL, 0.02 M) and Martin Sulfurane dehydrating agent (478 mg, 0.67 mmol, 2.0 equiv). The tube was sealed and placed in a 60° C. oil bath for 1 h. The reaction mixture was cooled to ambient temperature (23° C.), concentrated under reduced pressure and purified by automated flash chromatography (5→30% EtOAc/hexanes) to afford cyclohexene lactone (+)-28 (69 mg, 79% yield over 2 steps) as a single regioisomer (>19:1 rr, based on 1H-NMR analysis of the crude mixture).
(+)-28: clear colorless oil; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.41; [α]D20.3=+66.91 (c=0.17, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; (NMR data is provided for two diastereomers) 1H NMR (500 MHz, CDCl3) δ 5.40 (s, 2H), 4.43-4.33 (m, 2H), 4.08-3.96 (m, 2H), 3.15-3.02 (m, 2H), 2.82-2.69 (m, 2H), 2.16-1.85 (m, 9H), 1.80-1.49 (m, 7H), 1.47-1.38 (m, 2H), 1.31-1.23 (m, 2H), 1.20-1.05 (m, 4H), 0.91 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.82 (s, 3H), 0.79 (s, 3H), 0.77 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 179.24, 179.19, 148.16, 148.03, 116.76, 116.67, 72.81, 72.79, 48.02, 47.72, 40.46, 40.37, 39.00, 38.95, 37.83, 37.82, 36.26, 36.23, 35.97, 35.93, 33.38, 33.18, 31.66, 31.64, 31.46, 31.39, 21.58, 21.57, 21.18, 20.98, 19.61, 19.46; IR (thin film): 2932, 2860, 1769 cm−1; HRMS (ESI+) m/z calcd for C17H26LiO2 [M+Li]+: 269.2093, found: 269.2107.
(1R,3aR,7aS)-5-(1,3,3-trimethylcyclohexyl)-1,3,3a,6,7,7a-hexahydroisobenzofuran-1-yl acetate ((+)-29): To a solution of cyclohexene lactone (+)-28 (20 mg, 0.076 mmol, 1.0 equiv) in CH2Cl2 (1.3 mL, 0.06 M) was added DIBAl-H (1.0 M solution in CH2Cl2, 92 μL, 0.092 mmol, 1.2 equiv) dropwise at −78° C. The reaction mixture was stirred at −78° C. for 4 h and carefully quenched in sequence with 92 μL MeOH and saturated aqueous Rochelle's salt (2.6 mL). The dry-ice bath was removed and the mixture was allowed to slowly warm to ambient temperature (23° C.). Subsequently, the reaction mixture was filtered through a pad of Celite, extracted with CH2Cl2 (3×10 mL), washed with brine (10 mL), and concentrated under reduced pressure. The crude lactol was of sufficient purity (>95% as judged by 1H-NMR) to be carried on directly to the next step.
To a solution of the above crude lactol in anhydrous pyridine (0.8 mL) was added DMAP (2.0 mg, 0.015 mmol, 0.2 equiv) and Ac2O (36 μL, 0.38 mmol, 5.0 equiv). The reaction mixture was stirred at ambient temperature (23° C.) for 14 h, poured into saturated aqueous NaHCO3 solution (10 mL), and the aqueous layer was extracted with CH2Cl2 (3×10 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (0→30% EtOAc/hexanes) afforded acetyl lactol (+)-29 (14 mg, 62% yield over 2 steps) as a clear colorless oil and as a single diastereomer (>19:1 dr, based on 1H-NMR analysis of the crude mixture). TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.51; [α]D20.3=+50.91 (c=0.11, CHCl3); IR (thin film): 2939, 2861, 1744 cm−1; HRMS (ESI+) m/z calcd for C19H30LiO3 [M+Li]+: 313.2355, found: 313.2367. Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; The diastereomeric mixture was further separated by semi-preparative reverse-phase HPLC (100×21.20 mm, 5 μm; linear gradient, 5→95% CH3CN/H2O with 0.1% of NH4HCO3 over 20 min, 10 mL/min) to yield (+)-29a (TR=5.7 min) and (+)-29b (TR=6.8 min).
(+)-29a: 1H NMR (500 MHz, CDCl3) δ 6.03 (s, 1H), 5.46 (dd, J=4.6, 1.9 Hz, 1H), 4.27 (t, J=8.2 Hz, 1H), 3.70 (t, J=7.6 Hz, 1H), 2.96 (p, J=7.3 Hz, 1H), 2.35-2.27 (m, 1H), 2.19-2.12 (m, 1H), 2.06 (s, 3H), 1.99-1.90 (m, 1H), 1.80 (dt, J=12.9, 4.7 Hz, 1H), 1.73-1.67 (m, 1H), 1.66-1.24 (m, 5H), 1.21-1.07 (m, 1H), 1.03 (d, J=14.0 Hz, 1H), 0.92 (s, 3H), 0.87 (s, 3H), 0.82 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.48, 145.36, 117.37, 103.60, 74.63, 47.76, 40.20, 38.75, 37.58, 36.00, 35.72, 33.13, 31.38, 31.20, 26.77, 25.97, 21.33, 20.93, 19.36.
(+)-29b: 1H NMR (500 MHz, CDCl3) δ 6.01 (s, 1H), 5.43 (dd, J=4.5, 2.0 Hz, 1H), 4.26 (t, J=8.3 Hz, 1H), 3.61 (t, J=7.9 Hz, 1H), 2.98 (dt, J=13.4, 7.3 Hz, 1H), 2.26 (td, J=7.4, 3.7 Hz, 1H), 2.09 (dt, J=16.9, 4.5 Hz, 1H), 2.05 (s, 3H), 2.01-1.79 (m, 2H), 1.67-1.52 (m, 2H), 1.46-1.23 (m, 4H), 1.18-1.06 (m, 2H), 0.99 (d, J=13.9 Hz, 1H), 0.91 (s, 3H), 0.86 (s, 3H), 0.84 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 170.51, 145.39, 117.40, 103.75, 74.65, 47.47, 40.12, 38.69, 37.57, 35.97, 35.68, 32.93, 31.38, 31.14, 26.76, 25.97, 21.32, 20.73, 19.21.
((3S,4R)-1′-methyl-[1,1′-bi(cyclohexan)]-1-ene-3,4-diyl)bis(methylene) diacetate (+)-S6): To a solution of the cyclohexene lactone (−)-26 (27 mg, 0.12 mmol, 1.0 equiv) in anhydrous THF (1.5 mL, 0.08 M) was added LiAlH4 (2.0 M solution in THF, 0.15 mL, 0.30 mmol, 2.5 equiv) dropwise at 0° C. After stirring for 1 h, the ice bath was removed and the mixture was allowed to warm slowly to ambient temperature (23° C.) over 30 min. Upon consumption of starting material (as judged by TLC), the reaction mixture was cooled to 0° C. and carefully quenched in sequence with 12 μL H2O, 12 μL 25% aqueous NaOH, and 30 μL H2O. The ice bath was removed and the mixture was allowed to warm slowly to ambient temperature (23° C.). Subsequently, anhydrous MgSO4 was added and the reaction mixture was vigorously stirred for 30 min, filtered through a pad of Celite and concentrated by rotary evaporation. The crude diol was of sufficient purity (>95% as judged by 1H-NMR) to be carried on directly to the next step.
To a solution of the above crude diol in anhydrous pyridine (1.0 mL, 0.1 M) was added DMAP (2.0 mg, 0.012 mmol, 0.1 equiv) and Ac2O (120 μL, 1.28 mmol, 10.0 equiv). The reaction mixture was stirred at ambient temperature (23° C.) for 24 h, poured into saturated aqueous NaHCO3 solution (10 mL), and the aqueous layer was extracted with CH2Cl2 (3×10 mL). The combined organic layer was washed with brine (10 mL), dried over MgSO4, filtered, and concentrated under reduced pressure. Purification by automated flash chromatography (0→30% EtOAc/hexanes) afforded cyclohexene diacetate (+)-27b (37 mg, 99% yield over 2 steps) as a single diastereomer (>19:1 dr, based on 1H-NMR analysis of the crude mixture). (+)-27b: clear colorless oil; TLC (EtOAc/hexanes, 1:4 v/v), Rf=0.53; [α]D18.5=+31.52 (c=0.33, CHCl3); Absolute stereochemistry was assigned by analogy to cyclohexenol lactone (−)-21b; 1H NMR (500 MHz, CDCl3) δ 5.34 (dt, J=4.0, 1.6 Hz, 1H), 4.13-3.96 (m, 4H), 2.70-2.61 (m, 1H), 2.17-2.08 (m, 1H), 2.05 (s, 3H), 2.03 (s, 3H), 2.00-1.92 (m, 1H), 1.70-1.62 (m, 4H), 1.63-1.52 (m, 1H), 1.49-1.38 (m, 3H), 1.38-1.29 (m, 3H), 1.28-1.18 (m, 2H), 0.92 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 171.25, 171.15, 146.01, 118.56, 65.48, 65.11, 38.70, 36.59, 36.41, 36.30, 34.56, 26.47, 23.29, 23.02, 22.72, 21.13, 21.11; IR (thin film): 2930, 2856, 1742, 1235 cm−1; HRMS (ESI+) m/z calcd for C19H31O4 [M+H]+: 323.2222, found: 323.2232.
1′,3′,3′-trimethyl-[1,1′-bi(cyclohexan)]-1-ene ((±)-S7): To an oven-dried round-bottomed flask was added zinc powder (3.5 g, 53.5 mmol, 25 equiv) and LiCl (900 mg, 21.4 mmol, 10.0 equiv) with a large bore vacuum adapter with Teflon lined seal for addition of liquids via syringe. The flask and solids were then carefully flame-dried under high vacuum (using a vacuum/N2 double manifold) by repeated heating and cooling cycles with swirling of the solids to avoid the solids from clumping. After the final cycle of heating, the solids were allowed to cool for 3 min under N2, and then anhydrous THF (40.0 mL, 0.05 M) was slowly and carefully added with vigorous stirring. After addition of THF, 1,2-dibromoethane (0.4 mL, 4.28 mmol, 2.0 equiv) was added which sometimes led to an exotherm simultaneous with rapid gas evolution, which needs to be controlled. Pressure fluctuations generated upon addition of 1,2-dibromoethane were controlled by momentarily switching the manifold from N2 to vacuum. [Note: If gas evolution was not observed upon addition of 1,2-dibromoethane, a heat gun was employed to initiate gas evolution]. The solution was allowed to cool at ambient temperature over ˜5 min before being further cooled to 0° C. A solution of cyclohexanone S5 (0.22 mL, 2.14 mmol, 1.0 equiv) in THF (1.0 mL) was added dropwise by syringe followed by slow addition of allyl chloride (±)-20b (1.7 g, 10.7 mmol, 5.0 equiv) in THF (2.4 mL) via syringe pump at a rate of 1.0 mL/h. During this time, the reaction mixture was allowed to slowly warm to ambient temperature (23° C.). The reaction mixture was then quenched with saturated NH4Cl solution (60 mL). The organic phase was separated and the aqueous phase was further extracted with EtOAc (3×60 mL). The organic layers were combined, washed with brine (20 mL), dried with MgSO4, and concentrated under reduced pressure to afford dimethyl cyclohexenol (±)-S6 as a single diastereomer (>19:1 dr, based on H-NMR analysis of the crude mixture). This material was of sufficient purity (>95% as judged by 1H-NMR) to be carried directly to the next step without purification.
To a solution of (±)-S6 (600 mg, 2.7 mmol, 1.0 equiv) in absolute EtOH (55.0 mL, 0.05M) and under an atmosphere of N2 was added Pd(OH)2 on carbon (758 mg, 20 wt %, 5.4 mmol, 2.0 equiv). A H2 balloon was attached to the flask and after three consecutive cycles of evacuation (under high vacuum) and H2 purge, the mixture was stirred for 15 h. An aliquot was removed for H-NMR analysis to ensure complete hydrogenation of the cyclohexene moiety. The reaction mixture was filtered through a pad of Celite, washed with EtOAc, concentrated under reduced pressure, and taken on directly to the next step without purification.
A glass tube was charged with the above crude alcohol (45 mg, 0.20 mmol, 1.0 equiv) in anhydrous CHCl3 (5.0 mL, 0.04 M) and Martin Sulfurane dehydrating agent (202 mg, 0.30 mmol, 1.5 equiv). The tube was sealed and placed in a 60° C. oil bath for 1 h. The reaction mixture was cooled to ambient temperature (23° C.), concentrated under reduced pressure and purified by automated flash chromatography using isocratic elution with HPLC grade n-hexane to afford cyclohexene (±)-S7 (41 mg, 87% yield over 3 steps).
(±)-S7: clear colorless oil; TLC (Hexanes), Rf=0.94; 1H NMR (500 MHz, CDCl3) δ 5.47 (dt, J=3.7, 2.3 Hz, 1H), 2.10-1.86 (m, 5H), 1.69-1.49 (m, 6H), 1.44-1.37 (m, 1H), 1.33-1.22 (m, 1H), 1.18-1.04 (m, 2H), 0.98 (d, J=13.9 Hz, 1H), 0.92 (s, 3H), 0.87 (s, 3H), 0.86 (s, 3H); 13C NMR (126 MHz, CDCl3) δ 143.86, 118.08, 48.11, 40.82, 38.71, 36.60, 33.32, 31.82, 31.60, 27.07, 25.92, 24.92, 23.74, 22.79, 19.76; IR (thin film): 2978, 2951, 2866 cm−1; A satisfactory high-resolution mass spectrum of the parent ion could not be obtained for this compound.
5-(1,3,3-Trimethylcyclohexyl)hexahydroisobenzofuran-1-yl acetate (33): Martin sulfurane (1.20 g, 1.78 mmol, 5.00 equiv) was weighed out to a flame dried RB flask in a N2 filled glovebox. The flask was sealed with a septum and removed from the glovebox. A solution of the crude alcohol 21b (100 mg, 1.78 mmol, 1.00 equiv) in dry CHCl3 (7.00 mL) was added to the reaction flask and the resulting mixture heated to 70° C. for 2 hours. After complete reaction, the mixture was concentrated, filtered through a short pad of silica (30% EtOAc:Hexanes), concentrated, and used crude in the next step.
The lactone (20 mg of crude) was taken up in CH2Cl2 (1.3 mL) and cooled to −78° C. DIBAL (16.0 mL, 91.0 mmol, 1.20 equiv) was added dropwise at this temperature and the reaction stirred for 4 hours. Upon completion, the mixture was successively quenched with H2O (4 mL), NaOH (15% aqueous, 4 mL) and H2O (10 mL). The mixture was stirred for 30 minutes then a spatula tip of MgSO4 was added and stirred for an additional 30 minutes. The resulting heterogeneous mixture was filtered through Celite and concentrated. Material was used without further purification.
The crude lactol was dissolved in pyridine (1.00 mL) and DMAP (1.00 mg, 8.00 mmol, 0.10 equiv) was added. Ac2O (71.8 mL, 0.76 mmol, 10.0 equiv) was added dropwise and the reaction allowed to stir overnight at room temperature. The mixture was quenched with NaHCO3 (sat. aqueous) and extracted with CH2C2. The combined organics were washed with brine, dried over MgSO4, filtered, and concentrated. Purification of the resulting residue by silica gel chromatography (10-40% Et2O:Hexanes) provided 23.4 mg (28% yield, 5 steps) of the title compound 33 as a mixture of diastereomers as a clear colorless oil. Due to spectral complexity, integrations are not corrected and are listed for all diastereomers. Rf=0.51 (20% EtOAc:Hexanes). IR (film) 2945 (s) 2864 (m), 2361 (w), 1745 (s), 1462 (w), 1364 (m), 1236 (s), 1103 (m), 1007 (m), 964 (m), 930 (m), 905 (m) cm 1. 1H NMR (600 MHz, CDCl3) δ 6.01 (s, 2H), 5.46-5.41 (m, 2H), 4.26 (td, J=8.3, 2.1 Hz, 2H), 3.68 (t, J=7.6 Hz, 1H), 3.61 (t, J=7.9 Hz, 1H), 3.01-2.91 (m, 2H), 2.34-2.22 (m, 3H), 2.17-2.02 (m, 10H), 2.00-1.87 (m, 6H), 1.87-1.74 (m, 3H), 1.72-1.53 (m, 10H), 1.53-1.24 (m, 5H), 1.18-1.07 (m, 8H), 1.05-0.97 (m, 7H), 0.91 (d, J=1.7 Hz, 3H), 0.86 (d, J=1.6 Hz, 3H), 0.84 (s, 2H), 0.81 (s, 2H). 13C NMR (150 MHz, CDCl3) δ 170.79, 170.77, 146.0, 116.5, 116.4, 104.0, 103.9, 75.0, 74.4, 48.5, 47.7, 43.23, 43.17, 40.6, 40.5, 38.83, 38.82, 36.8, 36.5, 36.3, 33.4, 31.6, 31.5, 23.8, 23.1, 22.64, 22.62, 21.6, 19.7, 19.5. HRMS (ESI): Calcd for C19H30O3Na1 [M+Na]+: 329.2087, Found: 329.2093.
Compound 34: The tertiary alcohol 21b (76.0 mg, 0.27 mmol, 1.00 equiv) was added to a flame dried reaction flask and dissolved in CH2Cl2 (1.00 mL). The resulting solution was cooled to 0° C. and Et3N (190 mL, 1.36 mmol, 5.00 equiv) was added. SOCl2 (78.9 mL, 1.08 mmol, 4.00 equiv) was added dropwise and the reaction was stirred for 1 hour at 0° C. Upon complete reaction, as judged by TLC, the mixture was concentrated. The residue was taken up in 30% Et2O:Hexanes and passed through a short pad of silica (30% Et2O:Hexanes) and concentrated. The resulting yellow oil was used in the next step without further purification.
The residue (20.4 mg, 78.0 mmol, 1.00 equiv) was taken up in THF (1.00 mL) and cooled to 0° C. LAH (2 M in THF, 78.0 mL, 156 mmol) was added and the reaction stirred for 30 minutes at 0° C. The mixture was sequentially quenched with 6 mL H2O, 6 mL NaOH (15% aqueous), and 18 mL H2O then stirred for 30 minutes while warming to r.t. A spatula tip of MgSO4 was added and the mixture stirred an additional 30 minutes. The heterogeneous mixture was filtered through Celite, concentrated, and used in the next step without purification.
The crude diol was dissolved in pyridine (1.00 mL) and DMAP (1.00 mg, 7.80 mmol, 0.10 equiv) was added. Ac2O (74.0 mL, 0.78 mmol, 10.0 equiv) was added and the reaction stirred overnight at r.t. The mixture was then quenched with NaHCO3 (sat. aqueous) and extracted with CH2Cl2 (3×1 mL). The combined organics were washed with brine, dried, filtered and concentrated. Purification by silica gel chromatography provided 16.3 mg (27% yield, 3 steps) of the bisacetate 34 as a mixture of four isomers. Rf=0.49 (30% Et2O:Hexanes, PAA). IR (film) 2947 (m), 2919 (m), 2865 (m), 1740 (s), 1462 (w), 1451 (w), 1366 (m), 1230 (s), 1034 (m). 1H NMR (400 MHz, CDCl3) δ 5.53-5.33 (m, 1H), 4.15-3.91 (m, 4H), 2.74-2.48 (m, 1H), 2.30-2.09 (m, 2H), 2.05 (s, 3H), 2.03 (s, 3H), 1.99-1.76 (m, 1H), 1.74-1.60 (m, 1H), 1.60-1.48 (m, 2H), 1.48-1.35 (m, 1H), 1.28 (m, 1H), 1.18-1.05 (m, 2H), 1.05-0.95 (m, 1H), 0.95-0.71 (m, 9H). HRMS (ESI): Calcd for C21H34O4Na1 [M+Na]+: 373.2349, Found: 373.2344.
In this example, methods for synthesizing gracilin cores and representative gracilin derivatives of the invention are described.
Synthesis of Starting Materials
(R)-5-oxo-2,5-dihydrofuran-2-yl acetate (35): Synthesized according to known literature procedure (Tao, D. J.; Slutskyy, Y.; Overman, L. E. J. Am. Chem. Soc. 2016, 138, 2186). 5-Hydroxyfuran-2(5H)-one (Moradei, O. M. and Paquette, L. A. Org. Synth. 2003, 80, 66) (7.97 g, 89.9 mmol) was added to a flame dried reaction flask under N2 and suspended in vinyl acetate (82.0 mL). Lipase AK (5.50 g) was added and the reaction mixture stirred for 8 days at room temperature. Upon complete reaction, the mixture was filtered through Celite and the filtrate concentrated. The residue was purified by silica gel chromatography to yield 10.9 grams (96% yield, 93:7 er) of the title compound as a pale yellow liquid. Spectroscopic data was in agreement with previously reported data. The enantiomeric purity was determined by HPLC analysis using a chiral column (Chiralpak AD-H column, 22° C., 1.0 mL/min, 90:10 Hexanes:Isopropanol, 254 nm, tminor=12.273 min, tmajor=13.853 min).
(R)-5-(naphthalen-2-yloxy)furan-2(5H)-one (36): Procedure was adapted from known literature procedure (Tao, D. J.; Slutskyy, Y.; Overman, L. E. J. Am. Chem. Soc. 2016, 138, 2186). Pd(PPh3)4 (3.23 g, 2.79 mmol, 0.05 equiv) was weighed out to a flamed dried round bottom flask in an N2 filled glovebox. The flask was capped with a septum and removed from the glovebox. The yellow solid was dissolved in CH2Cl2 (475 mL) at room temperature. 2-naphthol (12.1 g, 83.8 mmol, 1.50 equiv) was added to the reaction flask followed by (R)-5-oxo-2,5-dihydrofuran-2-yl acetate 35 (7.94 g, 55.9 mmol, 1.00 equiv). The resulting solution was allowed to stir for 10 hours at room temperature over which time the reaction underwent several color changes from yellow to orange, and finally dark garnet. The reaction mixture was concentrated and purified directly by silica gel chromatography (10-100% EtOAc:Hexanes) to yield the 7.58 grams (60% yield, 94:6 er) of the title compound as a white solid. This material could be recrystallized from CH2Cl2:Hexanes to a single enantiomer if desired. Spectroscopic data was in agreement with previously reported data. 1H NMR (600 MHz, CDCl3) δ 7.83 (app t, J=9.0 Hz, 3H), 7.59 (d, J=1.9 Hz, 1H), 7.52 (t, J=7.5 Hz, 1H), 7.49-7.41 (m, 2H), 7.28 (dd, J=8.7, 2.3 Hz, 1H), 6.56 (s, 1H), 6.39 (d, J=5.7 Hz, 1H). 13C NMR (150 MHz, CDCl3) δ 170.1, 154.3, 149.9, 134.2, 130.5, 130.1, 127.9, 127.5, 126.9, 125.5, 125.1, 118.7, 111.8, 100.9. The enantiomeric purity was established by HPLC analysis using a chiral column (Chiralpak AD-H, 22° C., 1.0 mL/min, 90:10 Hexanes:Isopropanol, 254 nm, tmajor=11.292 min, tminor=15.236 min).
(S)-5-(naphthalen-2-yloxy)furan-2(5H)-one (37): Synthesized according to known literature procedure (Trost, B. M. and Toste, F. D. J. Am. Chem. Soc. 2003, 125, 3090). The spectroscopic data was in agreement with the R-enantiomer prepared above. The enantiopurity was established by HPLC analysis using a chiral column (Chiralpak AD-H, 22° C., 1.0 mL/min, 90:10 Hexanes:Isopropanol, 254 nm, tminor=11.648 min, tmajor=15.663 min).
Diels-Alder Cycloaddition Optimization
General procedure for Diels-Alder cycloaddition optimization: The desired lactone (0.25 mmol, 1.00 equiv) was added to a flame dried one-dram scintillation vial under an atmosphere of dry N2. Toluene (1.00 mL) was added to the reaction vessel followed by 2-trimethylsiloxy-1,3-butadiene (equiv as stated in table below). The scintillation vial was sealed with a screw cap and heated to 100° C. for 16 h.
Upon cooling to 23° C., the solvent was removed under reduced pressure and the resulting residue taken up in THF (575 ml). HCl (575 ml, 1 M, aqueous) was added and the biphasic mixture stirred vigorously for 30 minutes. At the completion of the reaction, the phases were separated and the aqueous layer extracted with EtOAc (3×1 mL). The combined extracts were washed with brine, dried over MgSO4, filtered, and concentrated. 1,3,5-Trimethoxybenzene was added as an internal standard to determine the yield of each reaction.
iPr
aRegioisomeric ratio (rr) determined by 1H NMR analysis of the crude reaction mixture with a calibrated internal standard.
bEnantiospecificity determined by HPLC analysis with a chiral column.
Alternative Synthetic Route
(3R,3aR,7aS)-3-(naphthalen-2-yloxy)tetrahydroisobenzofuran-1,5 (3H,4H)-dione (40): (R)-5-(naphthalen-2-yloxy)furan-2(5H)-one 36 (2.47 g, 10.9 mmol, 1.00 equiv) was added to aflame dried pressure vessel and the atmosphere purged with N2. PhMe (8.67 mL) was added followed by 2-trimethylsiloxy-1,3-butadiene 38 (4.73 mL, 27.2 mmol, 2.50 equiv). The pressure vessel was sealed with a screw cap and the mixture heated to 120° C. overnight. Upon cooling, the solvent was removed under reduced pressure and the residue taken up in THF (25 mL). HCl (25 mL, 1 M aqueous) was added and the resulting mixture was stirred vigorously for 30 minutes. The mixture was then transferred to a separatory funnel and the phases separated. The aqueous phase was extracted with Et2O (3×25 mL) and the combined extracts were washed first with NaHCO3 (15 mL, sat. aqueous) then brine (20 mL). The organic phase was dried over MgSO4, filtered, and concentrated. To crude compound as a 9:1 mixture of regioisomers. Purification by silica gel chromatography (10-60% EtOAc:Hexanes) followed by recrystallization yielded 1.35 grams (42% yield, 94:6 er) of the title compound as a white solid. Rf=0.23 (60% Et2O:Hexanes). M.P.=143-145° C. IR (film) 3430 (w), 3060 (m), 2960 (m), 2253 (w), 1788 (s), 1716 (s), 1632 (m), 1600 (m), 1511 (m), 1468 (m), 1391 (m), 1366 (m), 1347 (m), 1252 (s), 1157 (s), 1122 (s), 1074 (m) cm−1. 1H NMR (600 MHz, CDCl3) δ 7.80 (app d, J=8.8 Hz, 2H), 7.77 (d, J=8.2 Hz, 1H), 7.48 (t, J=7.5 Hz, 1H), 7.45-7.39 (m, 2H), 7.18 (dd, J=8.9, 2.5 Hz, 1H), 5.85 (s, 1H), 3.42-3.36 (m, 1H), 3.28-3.21 (m, 1H), 2.73 (dd, J=15.3, 6.7 Hz, 1H), 2.48-2.36 (m, 4H), 2.33-2.24 (m, 1H). 13C NMR (150 MHz, CDCl3) δ 207.9, 176.5, 153.7, 134.2, 130.3, 130.1, 127.8, 127.5, 127.0, 125.1, 118.6, 111.2, 103.7, 42.2, 39.6, 37.4, 36.8, 22.4. HRMS (ESI): Calcd for C18H16O4Na1 [M+Na]+: 319.0941, Found: 319.0942. Optical Rotation: [α]D25-201.6 (c=1.00, CHCl3) for an enantiomerically enriched sample of 94:6 er. The enantiomeric purity was established by HPLC analysis using a chiral column (Chiralpak AD-H column, 22° C., 1.0 mL/min, 90:10 Hexane:Isopropanol, 254 nm, tminor=39.708 min, tmajor=59.605 min). Absolute stereochemistry determined unambiguously via X-ray diffraction.
(3R,3aR,5S,7aS)-5-hydroxy-5-(1-methylcyclohexyl)-3-(naphthalen-2-yloxy)hexahydroisobenzofuran-1(3H)-one (41): Zn° powder (1.38 g, 21.1 mmol, 25.0 equiv) and LiCl (179 mg, 4.22 mmol, 5.00 equiv) were added to a flame dried RB flask and flame dried under high vacuum and cooled to room temperature for three cycles. Upon final cooling, THF (11.7 mL) was added and the resulting heterogeneous solution was stirred vigorously. 1,2-Dibromoethane (73.0 mL, 0.84 mmol, 1.00 equiv) was added and the solution was heated with a heat gun to initiate a gentle reflux. The solution was then cooled to 0° C. and the ketolactone 40 (250 mg, 0.84 mmol, 1.00 equiv) was added as a solution in THF (1.50 mL). 3-chloro-1-methylcyclohex-1-ene (Machacek, M. R.; Romeo, E. T.; Kattar, S. D.; Christopher, M.; Altman, M. D.; Northrup, A. B.; Ellis, J. M.; O'Boyle, B.; Donofrio, A.; Grimm, J.; Reutershan, M. H.; Childers, K. K.; Otte, R. D.; Cash, B.; Ducharme, Y.; Haidle, A. M.; Spencer, K.; Vitharana, D.; Wu, L.; Zhang, L.; Zhang, P.; Beaulieu, C.; Guay, D. WO2014/48065 A1, Apr. 3, 2014) (990 mL, 7.59 mmol, 9.00 equiv) was added over 5 hours via syringe pump as a solution in THF (2.40 mL). The reaction was allowed to warm to r.t. over the course of the addition and stirred overnight. Upon complete reaction, the mixture was quenched with NH4Cl (sat. aqueous) and extracted with EtOAc (3×10 mL). The combined extracts were washed with brine, dried over MgSO4, filtered, and concentrated. Purification by silica gel chromatography (10-90% EtOAc:Hexanes) provided 152 mg (46% yield) of the homoallylic alcohol as an inseparable mixture of epimers. Additionally, 52.5 mg (21% recovery) of the starting material was isolated and could be resubjected to the reaction conditions. Rf=0.38 (30% EtOAc:Hex). IR (film) 3499 (br, w), 2933 (s), 2863 (m), 1784 (s), 1658 (w), 1631 (m), 1600 (m), 1512 (m), 1467 (m), 1443 (m), 1252 (m), 1213 (m), 1177 (m), 1139 (m), 1121 (m), 962 (w), 927 (w) cm 1. Due to spectral complexity, integrations are not corrected and are listed for both diastereomers.
1H NMR (600 MHz, CDCl3) δ 7.81-7.72 (m, 12H), 7.50-7.36 (m, 12H), 7.20-7.15 (m, 4H), 5.92-5.86 (m, 3H), 5.85 (s, 1H), 5.77 (d, J=5.1 Hz, 3H), 5.61-5.54 (m, 3H), 3.26 (t, J=6.4 Hz, 1H), 3.21-3.10 (m, 4H), 3.05 (app dtd, J=12.6, 6.5, 2.6 Hz, 3H), 2.54-2.47 (m, 1H), 2.40-2.34 (m, 1H), 2.28 (s, 1H), 2.21-1.62 (m, 36H), 1.40 (app dd, J=17.3, 12.9 Hz, 8H), 1.34-1.19 (m, 15H), 1.00 (d, J=4.0 Hz, 8H). 13C NMR (150 MHz, CDCl3) δ 177.88, 177.81, 176.7, 154.21, 154.19, 154.0, 134.3, 130.9, 130.8, 130.2, 130.1, 130.0, 129.85, 129.8, 127.81, 127.79, 127.42, 127.39, 126.9, 126.76, 126.74, 124.9, 124.73, 124.72, 118.83, 118.79, 118.6, 111.0, 110.91, 110.88, 104.0, 103.9, 103.1, 81.0, 74.4, 74.3, 42.6, 42.5, 39.7, 39.5, 39.4, 36.72, 36.69, 36.2, 31.1, 30.5, 30.2, 30.04, 30.02, 29.9, 27.9, 27.6, 26.8, 25.2, 22.2, 22.0, 19.68, 19.66, 18.7, 18.24, 18.20. HRMS (ESI): Calcd for C25H28O4Na1 [M+Na]+: 415.1880, Found: 415.1881.
The ketone prepared above was taken up in EtOAc (12.3 mL) and Pd(OH)2/C (20% wt., 108 mg, 0.40 equiv) was added. The atmosphere was purged with an H2 balloon and the mixture allowed to stir overnight at room temperature. Upon complete reduction, the mixture was filtered through Celite and concentrated. The resulting residue was purified by silica gel chromatography (30% EtOAc:Hex) to yield 84.5 mg (55% yield) of the title compound as a white solid. Rf=0.46 (30% EtOAc:Hexanes). M.P.=156-160° C. IR (film) 3516 (br, w) 2931 (s), 2860 (m), 1777 (s), 1631 (w), 1600 (w), 1512 (w), 1468 (w), 1445 (m) 1251 (m), 1214 (m), 1177 (m), 1140 (m), 1046 (m), 922 (s) cm. 1′H NMR (600 MHz, CDCl3) δ 7.80-7.73 (m, 3H), 7.46 (ddd, J=8.1, 6.9, 1.0 Hz, 1H), 7.43 (d, J=2.3 Hz, 1H), 7.39 (ddd, J=8.0, 6.9, 1.0 Hz, 1H), 7.18 (dd, J=8.9, 2.5 Hz, 1H), 5.77 (s, 1H), 3.71 (app t, J=5.6 Hz, 1H), 3.18 (app t, J=6.4 Hz, 1H), 3.04 (app dt, J=12.8, 6.4 Hz, 1H), 2.19-2.14 (m, 1H), 2.12-2.03 (m, 2H), 1.72-1.64 (m, 4H), 1.62-1.56 (m, 2H), 1.48-1.23 (m, 6H), 0.94 (s, 3H). 13C NMR (150 MHz, CDCl3) δ 177.8, 154.2, 134.3, 130.1, 129.9, 127.8, 127.4, 126.8, 124.7, 118.8, 110.9, 104.0, 75.1, 63.0, 40.2, 39.4, 36.7, 30.54, 30.45, 30.0, 29.9, 26.7, 26.3, 22.1, 18.3, 17.3. HRMS (ESI): Calcd for C25H30O4Na1 [M+Na]+: 417.2036, Found: 417.2036. Absolute stereochemistry determined unambiguously via X-ray diffraction.
Mixture of (3R,3aR,7aS)-5-(1-methylcyclohexyl)-3-(naphthalen-2-yloxy)-3a,6,7,7a-tetrahydroisobenzofuran-1(3H)-one and (3R,3aR,7aS)-5-(1-methylcyclohexyl)-3-(naphthalen-2-yloxy)-3a,4,7,7a-tetrahydroisobenzofuran-1(3H)-one (42): The tertiary alcohol 41 (84.5 mg, 0.24 mmol, 1.00 equiv) was added to a flame dried reaction flask and dissolved in CH2Cl2 (1.00 mL). The resulting solution was cooled to 0° C. and Et3N (150 mL, 1.07 mmol, 5.00 equiv) was added. SOCl2 (62.5 mL, 0.86 mmol, 4.00 equiv) was added dropwise and the reaction was stirred for 1 hour at 0° C. Upon complete reaction, as judged by TLC, the mixture was concentrated and purified directly by silica gel chromatography (0-20% EtOAc:Hex) to provide 38.0 mg (47% yield) of the eliminated product as a pale yellow oil. Attempts to separate the product isomers were unsuccessful. Rf=0.65 (30% EtOAc:Hexanes). IR (film) 2928 (s), 2854 (m), 1787 (s), 1631 (w), 1600 (w), 1511 (w), 1467 (w), 1363 (w), 1251 (m), 1213 (m), 1178 (m), 1148 (m), 1120 (m), 972 (m), 947 (s), 845 (m) cm−1. Due to spectral complexity, integrations are not corrected and are listed for all diastereomers. 1H NMR (600 MHz, CDCl3) δ 7.82-7.74 (m, 10H), 7.49-7.43 (m, 7H), 7.39 (app ddt, J=8.2, 6.8, 1.4 Hz, 3H), 7.21 (app ddd, J=8.7, 6.1, 2.5 Hz, 3H), 7.10 (app ddd, J=23.7, 8.9, 5.5 Hz, 4H), 5.84 (s, 2H), 5.82 (s, 1H), 5.65 (t, J=4.5 Hz, 1H), 5.56-5.53 (m, 2H), 5.45 (dq, J=7.6, 3.1 Hz, 1H), 5.35 (s, 1H), 5.34-5.31 (m, 1H), 4.19-4.11 (m, 1H), 3.39-3.32 (m, 2H), 3.28-3.18 (m, 3H), 3.18-3.06 (m, 1H), 3.07-2.67 (m, 7H), 2.60 dddd, J=17.1, 4.8, 3.0, 1.7 Hz, 1H), 2.43 (dddt, J=17.1, 8.6, 4.1, 1.4 Hz, 1H), 2.34 (dd, J=15.9, 6.8 Hz, 1H), 2.30-2.22 (m, 2H), 2.19-1.82 (m, 13H), 1.82-1.51 (m, 19H), 1.51-1.17 (m, 39H), 0.97 (s, 9H). 13C NMR (150 MHz, CDCl3) δ 178.7, 177.9, 154.1, 154.1, 134.2, 130.0, 129.73, 129.71, 127.66, 127.31, 127.28, 126.64, 126.62, 124.64, 124.61, 118.73, 118.69, 111.0, 110.9, 105.1, 104.4, 74.5, 74.3, 42.9, 40.8, 39.0, 38.4, 36.7, 36.6, 36.3, 35.96, 35.95, 35.9, 35.82, 35.80, 35.7, 26.4, 26.31, 26.29, 24.1, 23.1, 22.68, 22.67, 22.43, 22.36, 21.1, 20.4. HRMS (ESI): Calcd for C25H28O3Na1 [M+Na]+: 399.1931, Found: 399.1934.
(3aR,5S,7aS)-5-hydroxy-5-(1-methylcyclohex-2-en-1-yl)hexahydroisobenzofuran-1(3H-one (43): Procedure adapted from literature report (Trost, B. M.; Crawley, M. L. Chem. Eur. J. 2004, 10, 2237). NaBH4 (9.80 mg, 0.26 mmol, 2.50 equiv) was added to a stirred solution of NaOH (20.4 mg, 0.51 mmol, 5.00 equiv) in H2O (500 mL) under an air atmosphere. After stirring for 5 minutes at room temperature, the naphthyloxylactone 40a (40.3 mg, 0.1 mmol, 1.00 equiv) was added as a solution in MeOH (1.00 mL). The mixture was allowed to stir overnight at room temperature then quenched with HCl (conc. 100 mL) and stirred for 1 hour. The reaction was diluted with CH2Cl2 (2 mL) and H2O (2 mL) and the layers separated. The organic phase was washed with brine, dried over MgSO4, filtered, and concentrated. The crude residue was purified by silica gel chromatography (10-50% EtOAc:Hexanes) to obtain 4.6 mg (18% yield) of the title compound as an inseparable mixture of epimers. 1H NMR (600 MHz, CDCl3) δ 5.87 (ddd, J=10.1, 5.3, 2.0 Hz, 1H), 5.59-5.53 (m, 1H), 4.28-4.23 (m, 1H), 3.94 (dd, J=8.9, 2.8 Hz, 1H), 2.78 (dq, J=11.1, 5.7, 5.2 Hz, 1H), 2.68-2.63 (m, 1H), 2.13-2.06 (m, 1H), 2.06-1.80 (m, 6H), 1.70 (ddd, J=14.7, 10.3, 3.6 Hz, 3H), 1.64-1.49 (m, 5H), 1.41-1.20 (m, 6H), 0.97 (d, J=4.8 Hz, 2H). 13C NMR (150 MHz, CDCl3) δ 178.7, 178.61, 131.07, 131.02, 130.6, 74.88, 74.85, 72.1, 72.0, 42.6, 42.5, 39.1, 39.0, 33.33, 33.28, 32.5, 31.5, 30.12, 30.05, 27.5, 26.6, 25.2, 22.1, 22.0, 19.68, 19.67, 18.7, 18.6.
In this example, the biological properties of representative gracilin A derivatives of the invention are described.
Materials
Carboxymethyl dextran (CM5) sensor chips, Hank's Balance Solution Surfactant P20 (HBS-EP) buffer (pH 7.4, 0.01 M 4-(2-hydroxyethyl)-1-piperazinedsdethanesulfonic acid (HEPES), 0.15 M NaCl, 3 mM EDTA, 0.005% polysorbate), amine coupling kit (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were supplied by BiacoreAB (Uppsala, Sweden). Percoll was obtained from Pharmacia (Uppsala, Sweden). Plastic tissue-culture dishes were purchased from Falcon (Madrid, Spain). The Pan T cell Isolation Kit (human) was purchased from MiltenyiBiotec (Germany). Active human CypA full-length protein was from Abcam. Tetramethylrhodamine methyl ester (TMRM), Thiol Tracker Violet, 5-(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) and Human IL-2 ELISA kit were purchased from Thermo Fisher Scientific (Madrid, Spain). Bovine Serum Albumin (BSA) and other chemicals were reagent grade and purchased from Sigma-Aldrich (Madrid, Spain). The composition of saline solution (PBS) used for human T lymphocytes purification was (mM): 137 NaCl, 8.2 Na2HPO4, 1.5 KH2PO4, 3.2 KCl and 2 EDTA. The composition of Umbreit saline solution was (mM): NaCl 119, MgSO4 1.2, NaH2PO4 1.2, NaHCO3 22.85, KCl 5.94, CaCl2 1. Glucose (1 g/L) was added to the medium. The pH was equilibrated between 7.2-7.3. Stock solutions of drugs were done in dimethylsulphoxide (DMSO).
Cell Culture
Human neuroblastoma SH-SY5Y cell line was purchased from American Type Culture Collection (ATCC), number CRL 2266. Cells were maintained in Dulbecco's Modified Eagle's medium: Nutrient Mix F-12 (DMEM/F-12) supplemented with 10% fetal bovine serum (FBS), glutamax, 100 U/mL penicillin and 100 μg/mL streptomycin at 37° C. in a humidified atmosphere of 5% CO2 and 95% air. Cells were dissociated weekly using 0.05% trypsin/EDTA. All reagents were provided by Thermo Fisher Scientific.
Human T lymphocyte isolation
Peripheral lymphocytes were isolated from human fresh heparinised blood from healthy volunteers as previously described (Alfonso, A., Botana, M. A., Vieytes, M. R. and Botana, L. M. Cell Signal, 2001, 13, 819-826). The blood was diluted in the same proportion with PBS plus EDTA (2 mM) previously equilibrated at room temperature. 4 mL of diluted blood were placed over 3 mL of isotonic percoll (57.5%) carefully avoiding the mixture of these two phases. Once the tubes were prepared they were centrifuged at 3000 rpm, 25 min at room temperature. After centrifugation, different phases were obtained and only the fraction that contained the population of lymphocytes was collected and washed three times with PBS-EDTA to removed percoll at 1500 rpm, 10 min, room temperature. Lymphocyte purity was always higher than 80%. T lymphocytes were purified from this population with a Pan T cell Isolation Kit. This is an indirect magnetic labelling system for the isolation of untouched T cells. T cell purity was always higher than 95%. Assessment of cell purity was performed by flow cytometry using a monoclonal antibody to human CD3 labelled with FITC. Viability (>95%) was determined by trypan blue exclusion. Pure T cells were maintained in RPMI (Roswell Park Memorial Institute) 1640 plus, 10% FBS, and plated in 24 plastic tissue-culture dishes in humidified 5% CO2 and 95% air atmosphere at 37° C.
Cell Viability Assay
The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay was used to determine the cytotoxicity of gracilin derivatives in T lymphocytes (Burton, J. D. Methods Mol. Med., 2005, 110, 69-78) and SH-SY5Y cells and the protective effect of these compounds against H2O2 insult in neuroblastoma cells as previously described (Leiros, M., Alonso, E., Sanchez, J. A., Rateb, M. E., Ebel, R., Houssen, W. E., Jaspars, M., Alfonso, A. and Botana, L. M. ACS Chem. Neurosci., 2014, S, 71-80). Human T lymphocytes were cultured in 24 well plates at the concentration of 2.5×106 cells/mL and exposed to different compound concentrations (0.01, 0.1, 1 and 10 μM) added to the culture medium. Human T cells were maintained in the presence of the pure compound at 37° C. in humidified 5% CO2/95% air atmosphere for 48 h.
Saponin was used as cellular death control and its absorbance was subtracted from the other data. After the incubation period with compounds, assay was performed. First cells were centrifuged (1500 rpm, 5 min, 4° C.). The pellets were resuspended in saline solution with MTT (250 μg/mL) and then incubated at 37° C. during 30 min. After washing off excess MTT twice with saline solution, cells were disaggregated with H2O and sonicated for 1 min and the absorbance of the colored formazan salt was measured at 595 nm in a spectrophotometer plate reader. All experiments were performed three times.
SH-SY5Y cells were seeded in 96-well plates at a density of 2.5×105 cells/mL and allowed to adhere for 24 h. At first, cells were treated only with compounds at concentrations ranging from 10-0.01 μM for 24 h to determine their possible cytotoxic effects. Experiments were carried out three times. For neuroprotective experiments, cells were treated with compounds at non-toxic concentrations (1 μM→1 nM) and 150 μM H2O2 for 6 h. Briefly, cells were washed three times with saline solution and 200 μL of MTT (500 μg/mL) dissolved in saline buffer were added to each well. Following 1 h of incubation at 37° C., the cells were disaggregated with sodium dodecyl sulphate at 5%.
Absorbance of formazan crystals was measured at 595 nm with a spectrophotometer plate reader. Saponin was used as death control and its absorbance was subtracted from the other data. All experiments were performed four times.
Binding Experiments: Surface Activation, Ligand Immobilization and Binding
A Biacore X SPR biosensor with Control Software and BIA evaluation software version 3.0 from Biacore (GE Healthcare, Uppsala, Sweden) was used to check the binding between molecules. Sensor surface activation and ligand immobilization were performed by using HBS-EP as running buffer at a flow rate of 5 μL/min and 25° C. CM5 sensor chips were used as surface where Cyp A or D was immobilized as ligand. The CM5 chip is a glass slide coated with a thin layer of gold with a matrix of carboxymethylated dextran covalently attached. The CM5 chip was activated using an amine coupling kit. Following manufacture instructions, a mixture (1:1, v/v) of EDC and NHS was applied for 2 min over the sensor chip. After activation, the ligand, 100 μg/mL of active human CypA protein dissolved in sodium acetate 10 mM at pH 4.5 was added to be immobilized over a CM5 sensor chip. Finally ethanolamine-HCl was injected to deactivate the remaining active esters and to avoid non-specific binding. Next, analytes (CsA as positive control or synthetic compounds) were added to check the binding between them and CypA. Once analytes were tested and interaction was observed, individual binding curves were analyzed by determining the kinetic constants of analytes-CypA binding, namely, the observed rate constant (Kobs), the association rate constant (Kass), the dissociation rate constant (Kdiss), and the kinetic equilibrium dissociation constant (KD). At equilibrium, by definition, Kdiss/Kass=KD. The pseudo-first order association rate constants Kobs (s−1) were determined for each compound concentration by using the 1:1 Langmuir association model of BiaEvaluation software (BiaCore, Uppsala, Sweden). Then a representation of Kobs against the corresponding concentration of each compound was done. These plots follow a linear correlation coefficient. From the equation of these representations, Kass, M−1 s−1, gradient of the plot, and Kdiss, s−1, intercept of the plot was obtained. Within these two values, the kinetic equilibrium dissociation constant KD for each analyte-CypA binding was obtained.
The duration of the sample injection was 2 min at 10 μL/min flow rate. Next, dissociation of bound molecules in HBS-EP buffer flow was studied. The bound drugs were removed from the chip surface before the next injection by adding 1 M Glycine-HCl at pH 2.5 for 1 min. The association phase was used to quantify the compound-CypA or D interactions. All experiments were performed four times.
Interleukin 2 release
Human T lymphocytes at the concentration of 1×106 cells/mL were plated in 24 well plates and pre-treated for 2 hours with gracilin derivatives at a concentration that did not produce cell viability decrease determined by MTT test. Then, cells were stimulated with Concanavalin A (Con A) at 50 μg/mL for 48 h to induce IL-2 release. The amount of IL-2 released to the culture medium was evaluated using Human IL-2 ELISA kit. Experiments were carried out four times.
Mitochondrial Membrane Potential Measurement
The mitochondrial membrane potential (ΔΨm) was assessed using the tetramethylrhodamine methyl ester (TMRM) probe. SH-SY5Y cells were seeded in 96-well plates at 2.5×105 cells/mL. After 24 h, cells were treated with compounds at different concentrations (1 μM→1 nM) and 150 μM H2O2 for 6 h. Then, cells were washed twice with saline solution and 1 μM TMRM was added to each well for 30 min at 37° C. After this time, cells were solubilized with DMSO and H2O at 50%. The fluorescence was monitored with a spectrophotometer plate reader (535 nm excitation and 590 nm emission). All experiments were performed four times.
Determination of Intracellular ROS Levels
Intracellular levels of reactive oxygen species (ROS) were determined with carboxy-H2DCFDA (5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate). This dye diffuses through the cellular membrane and is converted by cellular esterases to carboxy-H2DCFH (non-fluorescent). When carboxy-H2DCFH is oxidized by ROS, it becomes fluorescent (Halliwell, B. and Whiteman, M. Br. J. Pharmacol., 2004, 142, 231-255). Cells were seeded at a density of 2.5×105 cells/mL in 96-well plates and allowed to attach for 24 h. Following treatment with compounds at various concentrations (1 μM→1 nM) and 150 μM H2O2 for 6 h, SH-SY5Y cells were washed twice with serum-free culture medium. Then, carboxy-H2DCFDA 20 μM dissolved in serum-free culture medium was added to each well and the cells were incubated for 1 h at 37° C. After this incubation, the medium with the fluorescent dye was replaced with PBS and the plate was incubated for 30 min at 37° C. Fluorescence was read at 527 nm, with an excitation wavelength of 495 nm. All experiments were performed four times.
Glutathione Assay
Glutathione (GSH) levels were determined using Thiol Tracker Violet dye, which reacts with reduced thiols in cells. Reduced glutathione represents the majority of intracellular free thiols, so this probe can be used to estimate its levels in cells. Measurements were performed following manufacturer's protocol. Cells were seeded in 96-well plates at 2.5×105 cells/mL for 24 h and treated with compounds at various concentrations (1 μM→1 nM) and 150 μM H2O2. After 6 h, SH-SY5Y cells were washed twice with PBS and loaded with 100 μL of prewarmed Thiol Tracker Violet dye (10 μM) for 30 min at 37° C. The fluorescence was measured at 404 nm excitation and emission at 526 nm. All experiments were performed four times.
Mitochondrial Membrane Permeability Transition Pore (mPTP) Measurement
The blockage of mPTP by compounds was determined with the MitoProbe Transition Assay Kit following manufacturer's instructions. Briefly, SH-SY5Y human neuroblastoma cells were re-suspended in pre-warmed PBS/Ca+2 buffer at a final concentration of 1×106 cells/mL. Cells were loaded with 0.01 μM Calcein-AM and incubated at 37° C. for 15 min. Then, 0.4 mM CoCl2 and compounds at selected concentrations were added and incubated for 15 min at 37° C. Cyclosporine A (CsA) at 0.2 μM was used as positive control. After this incubation, cells were centrifuged and re-suspended in 100 μL of PBS. Just before analyzing, 1 mM tert-butyl hydroperoxide (TBHP) was added to the samples to induce pore opening. Fluorescence intensity was measured at 488 nm excitation and 517 nm emission wavelengths by flow cytometry using the ImageStreamMKII (Amnis Corporation, Merck-Millipore) and INSPIRER software. The fluorescence of 10,000 events was analyzed with IDEASR Application vs 6.0 (Amnis Corporation, Merck-Millipore). Experiments were carried out four times.
CypD Enzyme Inhibition Assay
The inhibition of the peptidyl-prolyl cis-trans isomerase (PPIase) activity of compounds was determined by following the rate of hydrolysis of N-succinyl-Ala-Ala-Pro-Phe-pnitroanilide by chymotrypsin. CsA was used as positive control. The assay was performed as previously described (Yan, W., Qing, J., Mei, H., Nong, J., Huang, J., Zhu, J., Jiang, H., Liu, L., Zhang, L. and Li, J. Bioorg. Med. Chem. Lett., 2015, 25, 5682-5686) with small modifications. The assay buffer (20 mM Tris-HCl, 50 mM NaCl, pH 7.8), CypD (1 nM) and the compounds at concentrations ranging from 0.001 to 10 μM were pre-cooled at 4° C. for 1 h. After that time, chymotrypsin at 0.4 mg/mL in 1 mM HCl was added to each well. The reaction was initiated by the addition of the peptide (0.1 mg/mL in 500 mM LiCl in THF). The absorbance at 380 nm was monitored every 30 sec for 5 min with a spectrophotometer plate reader. The blank rates of hydrolysis (in absence of CypD) were subtracted from the rates in the presence of the enzyme. The half-maximal inhibitory concentration (IC50) was calculated by fitting the data with a log(inhibitor) vs. response model of GraphPad Prism 5.0 software. All experiments were performed four times.
Statistical Analyses
Data are presented as mean±SEM. Differences were evaluated by one way ANOVA with Dunnett's post hoc analysis. Statistical significance was considered at p<0.05.
While illustrative embodiments have been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the invention.
This application claims the benefit of U.S. Application No. 62/638,856, filed Mar. 5, 2018, expressly incorporated herein by reference in its entirety.
This invention was made with Government support under Contract No. GM R37 052964 awarded by the National Institutes for Health and No. CHE-1546973 awarded by the National Science Foundation. The Government has certain rights in the invention.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2019/020812 | 3/5/2019 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2019/173382 | 9/12/2019 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 6638417 | Ishida et al. | Oct 2003 | B2 |
| 20160030388 | Baker et al. | Feb 2016 | A1 |
| Entry |
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