Patent Grant
10532126
This is the U.S. National Stage of International Patent Application No. PCT/EP2015/079502 filed on Dec. 11, 2015, which was published in English under PCT Article 21(2), and which in turn claims priority to European Patent Application Nos. 14197449.3, filed Dec. 11, 2014, and 15158224.4, filed Mar. 9, 2015.
The present invention relates to a three dimensional graft, particularly for repair of cranio-facial features and injured joints, and to a process of producing patient specific grafts using computer aided modelling and three-dimensional bio-printing with biocompatible inks.
Reconstruction of the nose and external ear in a patient specific manner are some of the greatest challenges in plastic surgery because of the complex, three-dimensional properties of the inner cartilage structure with regionally changing mechanical properties and overlaying skin. Auricular reconstruction is applicable to congenital deformities, microtia, melanoma related tissue sacrifice and injuries including accidents and severe burns. Ears are involved in approximately 90% of burns involving the head and neck. The mostly frequently used standard treatment for total auricular reconstruction in the United States and the European Union is based on a two- to three-stage surgical technique using autologous costal cartilage harvested from the sixth, seventh and eighth rib which is sculpted into an ear-like shape to the extent possible by the limited amount of harvested tissue. Sufficient amount of costal cartilage is generally achieved at age 10, delaying the reconstructive surgery. Another reconstruction method for ear reconstructive surgery is the use of silicone implants to avoid the need for costal cartilage harvesting. However placing an acellular scaffold under a thin layer of skin exposes the patient to a high risk of long-term complications. Additionally, it is impossible to provide for customized size and shape for each patient, and the reconstructed ear does not grow like the contralateral ear leading to asymmetry. Available reconstruction strategies involve several surgeries and their outcome is highly dependent of the expertise of the reconstructive surgeon. Donor side morbidity, collapse of the abdominal wall due to lack of costal cartilage support and severe pain related to the costal cartilage harvest are common complications.
Additionally, there is a large clinical need to repair osteochondral lesions, which occur as a result of sport injury, trauma and degenerative diseases such as osteoarthritis. Current methodologies to treat this involve transplantation of osteochondral grafts, which are either autologous or derived from bone banks. This treatment has several disadvantages including donor site morbidity, scarcity of donor tissue, surgical difficulty and the fact the graft consists of multiple pieces, each which can come loose or be mis-positioned in the height.
In view of this state of the art, the objective of the present invention is to provide methods and means for providing patient specific grafts that improve on the above mentioned deficiencies of the state of the art. This objective is attained by the subject matter of the claims of the present specification.
According to a first aspect of the invention, a method of providing a graft scaffold, particularly for use in a human patient, comprises the steps of:
According to another aspect of the invention, a method of providing a graft comprises the steps of:
According to yet another aspect of the invention, a graft as obtained or obtainable by any of the preceding aspects of the invention, or any of their specific embodiments, is provided, particularly for use in a method for craniofacial or joint repair.
According to yet another aspect of the invention, a method of craniofacial or joint repair comprises the computer model of the patient specific graft modified for three-dimensional additive manufacturing with cartilage specific printing mix comprising at least one cytocompatible polymer, at least one of minced tissue or other additive particle and cells, the crosslinking being provided by spontaneous or externally triggered reaction of reactive groups and molecules embedded in the co-extruded material or in bio-ink internally, at least one of these types being present on at least one of the polymer, minced tissue and cells to reconstruct functional and native cartilage like tissue grafts.
According to an aspect of the invention, a method of creating internal polymer gradients, porosity and support regions for grafts better suited for mechanical loading of the tissue graft manufactured is provided by additive manufacturing methods.
According to an aspect of the invention, a method of creating sacrificial external support structures to aid in the printing of overhanging features of grafts is provided, wherein the sacrificial polymer is co-deposited with the printing mix and functions as a reservoir of crosslinking initiators to polymerize the printing mix and is removed after polymerization.
A first aspect of the invention concerns a method of providing a graft scaffold, particularly in a human patient, comprising the steps of:
In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and particles. In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and fibres.
Fibres and/or particles, particularly when derived from cartilaginous tissue, may comprise factors aiding in supporting the growth of cells within the graft.
In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and both particles and fibres.
In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and cells. In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and cells and one or several growth factors. The inventors have surprisingly found that even in the absence of cartilaginous particles or fibres, the provision of gelling material and cells may be sufficient, particularly in presence of growth factors, to sustain cell viability and proliferation.
In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and particles and cells. In certain embodiments, the printing mix comprises an aqueous solution of a gelling polysaccharide and particles and fibres and cells.
In certain embodiments, said particles consist of, or comprise, tissue particles. In certain embodiments, said particles consist of, or comprise, cartilage particles. In certain embodiments, said particles consist of, or comprise, particles consisting of lyophilized cartilage tissue. In certain embodiments, said particles consist of, or comprise, human cartilage tissue. In certain preferred embodiments, said particles consist of, or comprise, autologous cartilage tissue. In certain preferred embodiments the particles can be clinical products of micronized matrix including BioCartilage, Amniofix, Alloderm-Cymetra, Cook Biotech Small Intestial Muscosa (SIS) particles. In certain preferred embodiments the particles can be hydroxyapatite or calcium phosphate.
In certain embodiments, the particles and/or fibres are made of a synthetic polymer, particularly a polymer selected from the group consisting of polymers, or polymers derived from, polyethylene glycol, polypropylene glycol, gel forming poloxamers F108, F127, F68, F88, polyoxazolines, polyethylenimine, polyvinyl alcohol, polyvinyl acetate, polymethylvinylether-co-maleic anhydride, polylactide, poly-N-isopropylacrylamide, polyglycolic acid, polymethylmethacrylate, polyacrylamide, polyacrylic acid, and polyallylamine or co-polymers of these or block-copolymers of these.
In certain embodiments, the particles and/or fibres comprise or are predominantly or exclusively composed of minced tissue. In certain embodiments, the minced tissue is derived from tissue selected from the group consisting of auricular cartilage, nasal cartilage, nucleus pulposus, meniscus, trachea, nasal cartilage, rib cartilage, articular cartilage, synovial fluid, vitreous humor, brain, spinal cord, muscle, connective tissues, small intestinal submucosa and liver. In certain embodiments, the minced tissue is in the range of from 5 μm-50 μm, 50-200 μm and 200-1000 μm or a combination of these.
In certain embodiments, said gelling polysaccharide is gellan gum, acylated and/or sulfated gellan gum. In certain embodiments, said gelling polysaccharide is selected from guar gum, cassia gum, konjac gum, Arabic gum, ghatti gum, locust bean gum, xanthan gum, xanthan gum sulfate, carrageen, carrageen sulfate, or a mixture of any of the above gelling polysaccharides.
In certain embodiments, said solution of a gelling polysaccharide comprises a cytocompatible polymer as an additive in addition to the gelling polysaccharide, particularly a cytocompatible polymer selected from the group consisting of alginate, alginate sulfate, gellan sulfate, carrageen, carrageen sulfate, guar gum, cassia gum, konjac gum, Arabic gum, ghatti gum, locust bean gum, xanthan gum, xanthan gum sulfate, heparin, fibrin, heparin sulfate, elastin, tropoelastin, chondroitin sulfate, dermatan sulfate, hyaluronic acid, hyaluronan sulfate, cellulose, dextran, dextran sulfate, poly-l-lysine, chitosan, silk and collagen.
In certain embodiments, the additive is comprised in combination with gellan gum, acylated and/or sulfated gellan gum.
Gellan gum is a water-soluble polysaccharide produced by the bacterium Pseudomonas elodea. The repeating unit of the polymer is a tetrasaccharide, which consists of two residues of D glucose and one of each residues of L-rhamnose and D-glucuronic acid. The repeat has the following structure: [D-Glc (β1→47D-GlcA(β1→)Djhbn-Glc(β877→u8ir)L-Rha(α1→3)]n
“Acylated gellan gum” is a term known in the art and refers to gellan that comprises acetyl in some or all oxygen 5′ positions and glycerylic acid in some of all oxygen 2′ positions of the glucose unit. See
In certain embodiments, the solution of a gelling polysaccharide comprises gellan gum or acetylated gellan gum, or a sulfation product of acylated gellan gum, as the gelling polysaccharide, and alginate, alginate sulfate, gellan sulfate, carrageen, and/or carrageen sulfate as a cytocompatible polymer additive.
In certain embodiments, an aqueous solution of a salt comprising monovalent, divalent and/or trivalent cations is added to said gelling polysaccharide to effect gelation.
In certain embodiments, said aqueous solution comprises between 10 and 150 mmol/l of divalent ions. In certain embodiments, said aqueous solution comprises strontium ions (Sr2+). In certain embodiments, said aqueous solution comprises barium ions (Ba2+). In certain embodiments, said aqueous solution comprises calcium ions (Ca2+).
In certain embodiments, said aqueous solution comprises a total of between 10 and 150 mmol/l of divalent ions. In certain embodiments, said aqueous solution comprises between 10 and 150 mmol/l of strontium ions (Sr2+), particularly between 15 and 50 mmol/l Sr2+. In certain embodiments, said aqueous solution comprises between 10 and 150 mmol/l of barium ions (Ba2+), particularly between 15 and 50 mmol/l Ba2+. In certain embodiments, said aqueous solution comprises between 10 and 150 mmol/l of calcium ions (Ca2+), particularly between 15 and 100 mmol/l Ca2+.
In certain embodiments, said aqueous solution comprises a total of between 10 and 150 mmol/l of Sr2+ and Ba2+, particularly between 15 and 50 mmol/l of Sr2+ and Ba2+. In certain embodiments, said aqueous solution comprises a total of between 10 and 150 mmol/l of Ca2+ and Ba2+, particularly between 15 and 50 mmol/l of Ca2+ and Ba2+. In certain embodiments, said aqueous solution comprises a total of between 10 and 150 mmol/l of Sr2+ and Ca2+, particularly between 15 and 50 mmol/l of Sr2+ and Ca2+.
In certain embodiments, said solution of a gelling polysaccharide comprises a monosaccharide sugar or disaccharide sugar, particularly glucose, mannose or arabinose, at physiologic osmolarity. This addition can be important to safeguard viability of the cells embedded in the printing mix.
In certain embodiments, said particles and/or fibres consist of, or comprise,
In certain embodiments, particles are formed from a biopolymer mentioned above in oil emulsion or by precipitation. In certain specific embodiments, such biopolymer is alginate.
In certain embodiments, said tissue particles are derived from tissue selected from the group consisting of auricular cartilage, nasal cartilage, nucleus pulposus, meniscus, trachea, nasal cartilage, rib cartilage, articular cartilage, synovial fluid, vitreous humor, brain, spinal cord, muscle, connective tissues, small intestinal submucosa and liver.
In certain embodiments, the cytocompatible polymer is a natural polymer.
In certain embodiments, the cytocompatible polymer is gellan gum of varying acylation degree, particularly acylation ranging between 100% to 10% acylation, with 100% being high, and optionally comprises an additive selected from the group consisting of alginate, alginate sulfate, heparin, fibrin, heparin sulfate, elastin, tropoelastin, chondroitin sulfate, dermatan sulfate, hyaluronic acid, hyaluronan sulfate, cellulose, dextran, dextran sulfate, poly-l-lysine, chitosan, silk and collagen of varying type and sulfated versions of these.
In certain embodiments, ≥90%, ≥95% or ≥98% of said particles are in the range of from 5 μm-1000 μm, particularly from 5 μm to 50 μm, 5 μm to 200 μm, 50 μm-200 μm or 200 μm to 1000 μm.
In certain embodiments, said fibers are sized in the range of 5 μm-50 μm and 50-500 μm in length having an aspect ratio ranging between 2-1000, particularly an aspect range of 10-500, more particularly 100-500, 100-1000, 200-1000 or from 500 to 1000. In certain embodiments, silk fibres are used having a diameter of 1 μm or less, and a length of 500 to 1000 μm or more. The aspect ratio for the purpose of the term's use in the context of the present specification is defined as the ratio of fiber length to diameter.
In certain embodiments, said mammalian cells are cartilage cells, cartilage precursor cells or stem cells capable of differentiating into cartilage precursor cells or cartilage cells.
In certain embodiments, the mammalian cells are selected from the group consisting of primary autologous chondrocytes, primary allogenic chondrocytes, chondroprogenitor cells, chondroblasts, mesenchymal stem cells, induced pluripotent stem cells and adipose-derived stem cells.
In certain embodiments, the printing mix comprises:
In certain embodiments, the printing mix comprises:
In certain embodiments, the printing mix is deposited together with a sacrificial polymer.
This allows the generation of overhanging structures, such as are especially important in shaping certain features of the nose and ear.
In certain embodiments, the printing mix is deposited onto a sacrificial polymer scaffold.
In certain embodiments, the sacrificial polymer scaffold is co-deposited with the printing mix.
In certain embodiments, the sacrificial polymer mix and/or scaffold comprises divalent cations or other agents of gelation/polymerization.
By diffusing out of the sacrificial polymer mix, into the printing mix, these cations or other agents of gelation/polymerization allow for a rapid formation of the three dimensional structure of the scaffold.
In certain embodiments, the three dimensional form and/or said sacrificial polymer scaffold is derived by 3-D-printing methods, particularly on the basis of a three dimensional computer model of a contralateral organ of said patient.
In certain embodiments, the three dimensional model is obtained by computed tomography, magnetic resonance imaging, laser scanning or utilizing three dimensional cameras.
In certain embodiments, the computer model is created to support load bearing in gradients and to create internal structures for better cell survival and porosity.
In certain embodiments, the polymer scaffold is derived by additive manufacturing methods.
In certain embodiments, the additive manufacturing method is ink jet printing, bioprinting, extrusion printing or layer-by-layer method.
In certain embodiments, the polymer scaffold is characterized by internal polymer gradients, porosity and support regions.
In certain embodiments additional polymers are added to increase the matrix liquid viscosity so that the ink can be extruded consistently and is not blocked due to filter pressing phenomena.
In certain embodiments, the sacrificial polymer is removed prior or subsequent to said cell culture step.
In certain embodiments, the tissue particles and/or bioink comprise a growth factor or a combination of growth factors, particularly selected from BMP-2, BMP-7, TGF-β1, TGF-β2, TGF-β3, and/or FGF-2, and/or mitogenic factors, particularly IGF-1, to promote healing and regeneration.
In certain embodiments, growth factors can be directly loaded into the bioink mixture. In certain embodiments, the concentration of said growth factor(s) is in the range of from 0.1-5 mg/ml, 5-50 ng/ml or 50-500 ng/ml of one growth factor or a combination of several growth factors. In certain embodiments, the growth factors are selected from BMP-2, BMP-7, TGF-β1, 2, 3, IGF-1 and/or FGF-2.
In certain embodiments, the printing mix, particularly the particles, comprise additional components, particularly components selected from growth factors, antioxidants, cytokines, drugs and biologics.
In certain embodiments, the sacrificial polymer concludes an agent for initiating the crosslinking, said agent being a monovalent, divalent and trivalent cation, enzyme, hydrogen peroxide, horseradish peroxidase, radiation polymerizable monomers such as lithium phenyl-2,4,6-trimethylbenzoylphosphinate.
In certain embodiments, crosslinking initiating groups are present in the printing mix, particularly selected from groups that participate in light exposure, cation-mediated crosslinking and enzyme-mediated crosslinking.
Another aspect of the invention concerns a method of providing a graft repair, comprising the steps of:
Another aspect of the invention concerns a graft scaffold obtainable by, or obtained by, the method according to any one of the preceding methods of the invention, or any specific embodiment or combination of features provided by the specific embodiments.
The present invention provides patient-specific craniofacial reconstructive grafts produced by additive manufacturing methods. The cartilage tissue graft abolishes the need for cartilage harvesting thus decreasing patient discomfort, reducing the surgical time and allowing better replication of shape, size and mechanical flexibility. Furthermore faster tissue regeneration and increased cell proliferation can be achieved to enhance the surgical recovery. For craniofacial applications this technique can be combined with currently used skin augmentation treatment (i.e. expanders) or other natural or synthetic skin grafts.
Printing
According to the present invention, patient specific auricular and nasal grafts are produced based on the three-dimensional scanned models from the patient by utilizing additive manufacturing methods such as but not limited to extrusion printing, inkjet printing and other layer-by-layer deposition methods. Clinical computed tomography (CT), magnetic resonance imaging (MRI) or other three dimensional imaging tools such as laser scanners, 3D cameras or combinations of these are used to produce the computerized model of the patient specific implant. For ear reconstruction, the image can be mirrored to produce a computational model precisely mimicking the contralateral ear for tissue graft production. For ear and nose reconstruction a library of graft models can be used to provide choice of grafts for the patient especially in a case where a normal contralateral scan cannot be performed. These methods can lead to better cosmetic and aesthetic results when specific size reductions tools are used to reduce the dimensions of the cartilage framework by the thickness of the skin layer to achieve a final graft of correct size. Additive manufacturing methods can be utilized in creation of these constructs in high precision and in sterile conditions. Furthermore internal support structures and porosity for cell survival in large constructs can be added in a patient-specific shape and/or stiffness depending on the patient's needs. Avascularized cartilage can be designed to host vascular structures for over laying skin and other tissues in its proximity to prevent necrosis. The printed cartilage framework can be used as a bioactive template for the construction of overlying tissues, releasing growth factors and other secretory molecules to enhance the viability of neighboring cells. This release can be specially designed by having sulfated polymers in the mixture to bind growth factors to the proximity of the cells and slowly releasing the molecules.
In certain embodiments, the 3D form can be created as a computer model to support load bearing in gradients and to created internal structures for better cell survival and porosity.
Material
The bioink material comprises at least one cytocompatible polymer and at least one of particles and cells, the crosslinking being provided by spontaneous or externally triggered reaction of reactive groups and molecules, at least one of these types being present on at least one of the polymer, minced tissue and cells. The cytocompatible polymers (hereinafter referred to as “the polymers”) for use in this method may be any suitable polymers with the necessary cytocompatibility, that is, their presence is not harmful to cells. They may be natural (biopolymers) or synthetic materials, or combinations of these. The necessary reactive groups allowing the crosslinking may be already present on the polymers, or the polymers may be modified to include such groups. Typical non-limiting examples of natural polymers include alginate, alginate sulfate, heparin, fibrin, heparin sulfate, elastin, tropoelastin, chondroitin sulfate, dermatan sulfate, hyaluronic acid, hyaluronan sulfate, cellulose, dextran, dextran sulfate, poly-l-lysine, chitosan, gelatin, gellan gum of varying acylation degree, gellan sulfate, guar gum, cassia gum, konjac gum, Arabic gum, ghatti gum, locust bean gum, xanthan gum, xanthan gum sulfate, carrageen, carrageen sulfate, silk and collagen of varying type. All sulfated versions of these polymers are included.
Typical non-limiting examples of synthetic polymers include, but are not limited to, polymers, or polymers derived from, polyethylene glycol, polypropylene glycol, polaxomers, poly oxazolines, polyethylenimine, polyvinyl alcohol, polyvinyl acetate, polymethylvinyl ether-co-maleic anhydride, polylactide, poly N-isopropylacrylamide, polyglycolic acid, poly methylmethacrylate, polyacrylamide, polyacrylic acid, and polyallylamine.
By “at least one” of the groups being present on at least one of the polymer, particles and cells, is meant that the added reactive groups may be present on all or any of these entities.
Particles incorporated to polymer solution can consist of but are not limited to extracellular matrix tissue particles, loaded or unloaded beads and fibers in size range between 5-500 microns.
Crosslinking
The formation of hydrogel based on the material combination, particles and cells can be initiated by many factors or agents, including but not limited to mono-, di-, trivalent cations, enzymes and radical initiators. Additionally, physical and physical-chemical methods may be employed, for example, treatment in low or high pH solution and different temperature regions during the manufacturing process.
In certain embodiments, either one of said printing mix and said polymer scaffold comprises reactive groups covalently attached thereto, particularly reactive groups facilitating linking of said printing mix, or its constituent components, to said particles, by crosslinking by spontaneous or externally triggered reaction, wherein reactive groups are present on at least one of the polymer, minced tissue and cells to reconstruct functional and native cartilage like tissue grafts.
Particles
The size of the minced tissue to be used may be any suitable size, but in a particular embodiment, it is from 5 microns-500 microns, so that it can be extruded without clogging the dispensing unit such as needle or valve. The minced tissue for use in the method may be any suitable tissue, but it is advantageously tissue of a similar or identical nature to that of the cartilage. Exemplary and non-limiting examples of suitable tissue include articular cartilage, nucleus pulposus, meniscus, trachea, nasal cartilage, rib cartilage, ear cartilage, synovial fluid, tracheal cartilage, vitreous humor, brain, liver, spinal cord, muscle, connective tissues and subcutaneous fat, intrapatellar fat pad, small intestinal submucosa. A particular example is tissue with high content of elastin and glycosaminoglycan, particular examples being any type of cartilage, nucleus pulposus and meniscus. The tissue may be minced by any suitable method, exemplary and non-limiting methods including homogenizing, cryomilling, dry milling, cutting, chopping, crushing and slicing. The tissue may be subject to decellularization to remove epitopes which can cause acute inflammatory responses and pathogens including HIV. Recently, decellularized tissues, that is, tissue in which the cells have been killed and their remnants removed, have attracted interest as scaffold material alternatives to simpler approaches where the scaffold is composed of a single material (Hoshiba et al. “Decellularized matrices for tissue engineering”. Expert Opinion on Biological Therapy. 2010; 10:1717-28). Tissue decellularization results in a scaffold of extracellular matrix ideally suited for regenerating injured or diseased tissue since it retains the high resolution architecture and biological cues necessary for recapitulation of function. Decellularization may be done, for example, by using detergents, hydrogen peroxide, sodium hydroxide and enzymes, RNase and DNase. Particles can be manufactured by methods such as but not limited to colloid formation by hydrophilic/hydrophobic interactions, two phase emulsions and in oil interfaces. Fibers can be manufactured by methods such as but not limited to electrospinning, fiber extrusion and fiber pulling. Particles and fibers of any kind may be minced by any suitable method, exemplary and non-limiting methods including homogenizing, cryomilling, dry milling, cutting, chopping, crushing and slicing. These additive tissue pieces, particles and fibers may be further modified with functional groups binding to carrier polymer or combination of these materials or treated to expose reactive groups for crosslinking. Furthermore growth factors, antioxidants and drug molecules may be loaded in or on the added polymers, tissue pieces, particles and fibers.
Cells
The use of the term “cells” in this description encompasses not only individual cells, particularly mammalian cells, more particularly human cells, most particularly autologous human cells, but also encompasses agglomerations of the described cells which form spheroids, pellets, and microtissues, which are well known to and commonly used by the art. The cells for use in the method are advantageously cells of a similar type as those present on the cartilage tissue. Typical non-limiting examples of suitable cell types include primary autologous chondrocytes, primary allogenic chondrocytes, chondroprogenitor cells, chondroblasts, mesenchymal stem cells, induced pluripotent stem cells and adipose-derived stem cells, neural crest derived stem cells.
Printing Mix Material
The term “printing mix” in the context of the present specification refers to an extruded mass comprising the key constituent components:
The composition of the printing mix material may be varied across a wide range, depending on the nature of the materials and the end-use. The polymers are typically present in a weight proportion of from 0.5-20%. When minced tissue, particles or fibers are present, they are typically present at a weight proportion of from 10-40% of dry polymers or equally 1-20% in total weight. When cells are present, they are typically used at concentrations of 3×106 cells/m1-50×106 cells/ml.
In addition to the major components hereinabove described, the crosslinkable material may include other materials, present to confer particular properties on the material. One particular example is elastin, which is abundant in auricular and nasal ECM to provide the elasticity of the tissue and other examples include growth factors, cytokines, drugs, biologics, siRNA, DNA, antioxidants such as polyphenols into the polymeric solutions, which could augment regeneration of the tissues. Added growth factors could be bound to sulfated polymers or unmodified polymer for enhanced delivery and effectivity in the proximity of the cells residing in the printing mix.
The printing mix material in its ready-to-use form is a readily thermally gelled state that can easily be applied to take desired shape in the manufacturing process. Powders of the molecules and lyophilized minced tissue, particles and fibers can be stored and sterilized separately. All the components can be combined before packaging or rehydrated just prior to use thus preserving the growth factors and proteins for long periods of time.
Shape
Patient specific tissue grafts are tailored for each patient or certain model catalogs can be created for situations where patient imaging is not desired or not possible. The three-dimensional model obtained from external ear and nose scans can be modified to contain internal support structures, gradient of polymers for versatile mechanical properties and porosity for enhanced cell survival in large constructs. Furthermore region could be tuned in terms of stiffness, growth factor cocktail and concentration, for example, to induce regional variations in cell proliferation. For example the periphery of the cartilage graft could be more porous or softer allowing more nutrient flow into the deep structure. Also the regional specificity and tissue types are found in these constructs, for example, in the lobe of the ear fat is the main tissue and is responsible for the mechanical properties. The regional properties and specified structures can be easily built in a layer-by-layer manner. In such a layered approach, the crosslinking mechanism would take place not only within individual layers, but also between adjacent layers, thus forming a completely integrated continuous structure. This can be achieved by initiating crosslinking in the periphery of the construct in contact with support structure containing the reactive molecule reservoir.
Support
Support structure can be co-deposited with the printing mix material to support overhanging structures, to initiate crosslinking or to prevent drying of the material during deposition. Support material can contain crosslinking factors including but not limited to mono-, di-, trivalent cations, enzymes and radical initiators. Additionally, physical and physical-chemical methods may be employed by support material interactions to modify the pH and molecule concentration. After the construct manufacturing the support structure can be eluted. Elution can be due to but not limited to temperature change, pH change or degrading molecules.
The result is a cartilage repair that is quick, effective and long-lasting. The longevity is an important factor in the graft to preserve the mechanical properties until sufficient ECM production of the cells has been achieved to produce a native cartilage-like structure.
Typical examples of the use to which the method of this disclosure may be put include:
The method of the invention is characterized by the following advantages:
The disclosure is further described with reference to the following figures and non-limiting examples, which depict particular embodiments.
Clinical computed tomography imaging was performed and the resulting computational three-dimensional object (
Using the same technique the inventors have demonstrated the printing of several cartilaginous structures including meniscus, intervertebral discs and nose. Two-component intervertebral disc grafts could be printed with two bioink compositions mimicking the nucleus pulposus and the annulus fibrosus.
Cartilage was harvested from the fresh bovine articular or auricular cartilage by removing thin layers of cartilage into a petri dish containing PBS and penicillin-streptomycin 1%. The harvested cartilage was transferred into cryomill (Retsch) and milled for three cycles in 30 Hz intensity. Milled cartilage was collected and lyophilized to obtain dry powder that could be sieved into the desired particle size range. These particles can be further loaded with growth factors or other molecules to enhance the proliferation and other cell responses. After loading the particles were lyophilyzed and cryopreserved to maximize the biomolecule availability for prolonged shelf life.
Printing mix material (“Bio-Ink”) was produced by combining gellan gum in 3.5% concentration with the alginate 3%. Gellan gum was dialyzed against ultrapure water to minimize the cation residues in the material. Dialysis was performed over three days in 70-80° C. ultrapure water changing the water one to two times a day. Gellan was further lyophilized to obtain a dry powder. Purified gellan gum was dissolved into glucose containing deionized water making it more cell compatible and alginate solution was added to obtain final concentration of polymers. The polymer blend was mixed with ECM particles and 6×106 cells/ml to obtain the final printing mix material. This printing mix material stimulated the cell proliferation significantly compared to positive control (
The printing mix material was printed onto a substrate and the support polymer Pluronic F127 was co-extruded to fill subsequent layer. Pluronic contained 20 mM of SrCl2 to initiate the bioink crosslinking upon contact with the ink. Cations diffused into the printing mix material due to osmotic balance and electrostatic forces which initiated the crosslinking. Structures were generated with 410 μm needles and 800 mm/minute feedrate. Pressure applied to extrusion syringe varied between 1.2-1.4 bar. After layer-by-layer deposition of the material into desired form, the sacrificial support Pluronic was eluted in a 20 mM SrCl2 bath for few minutes before the construct was transferred to 37° C. cell culture medium.
Bioink preparation: Gellan was added to D-glucose (300 mM) containing ultra-pure water at 90° C. to achieve a 3.5% solution, of which 85% was low-acyl gellan gum and 25% was high-acyl gellan gum. Alginate was added to the mixture to achieve 2.5% solution. The boiling flask was kept at 90° C. with agitation until the solution was homogeneous, typically for one hour. The homogeneous solution was cooled down to 30° C. prior the cell mixing. Briefly, the bovine chondrocytes (4×106 cells/ml) were mixed in the DMEM solution and added to the bioink in culture medium in 1:10 volume ratio to pre-crosslink the bioink. Mixing was performed until the solution reached room temperature and the printing syringes were loaded.
Gellan gum high acyl (GG-HA) and low acyl (GG-LA) compositions (
Base polymer gellan 3% with additive hyaluronan conjugated with tyramine 3% were mixed together to generate enzymatically crosslinkable hydrogel in the presence of horseradisch peroxidase (HRP) and hydrogen peroxide. Materials were dissolved into deionized water in presence of monosaccharide glucose in physiologic osmolarity, specifically 300 mM. Hydroxyapatite particles in concentration of 4% (w/v) were added to the polymer mixture. This bio-ink composition was further bioprinted in the presence of HRP and hydrogen peroxide when HRP was mixed either to the bio-ink in 1 unit/ml concentration or to the pluronic F127 30% mixture together with the hydrogen peroxide in 0.0012% concentration. Layer-by-layer constructed scaffold crosslinked immediately upon contact with the support structure. The support structure was eluted in the cold medium to decrease the negative effects of hydrogen peroxide in the presence of the cells. The structure was washed several times after to minimize the amount of hydrogen peroxide residues.
Gellan 3% was dissolved in deionized water and 10% (w/v) polymethyl metacrylate (PMMA) fibers were added as chopped eletrospun fibers into the gellan solution. Fibers were imaged with scanning electron microscopy to determine the fiber diameter to be approximately 2 micron. The fiber reinforced gellan was imaged before the shear (
An exemplary bioink is a blend of gellan and alginate mixed with human micronized Cartilage particles or HA particles (≤40 μm size). Upon addition of mono-, di- or trivalent cations, gelation (sol-gel transition) occurs as the helices aggregate into junction zones which are linked into a three dimensional network via the coiled part of the molecule. The printing process is divided into three stages namely bioink pre-printing, printing process and post-printing crosslinking. Initially the bioink was loaded into a syringe and the support polymer into a second syringe. At this stage, a small amount of cations were present in the bioink to increase viscosity and enhance printing properties. During the printing process the co-extruded of the support, cations diffused to the periphery of the printed structures to initiate the crosslinking. After the final structure is completed, the support can be eluted in 4° C. cation-supplemented medium.
The cation related viscosity enhancement and crosslinking properties can be investigated with rheology and mechanical testing. Rheological properties of the Bioink, Bioink+HA, and Bioink+Cartilage Particles were measured with an Anton Paar MCR 301 (Anton Paar, Zofingen, Switzerland) rheometer to determine the shear behavior and shear recovery. All of the bioink compositions showed shear thinning behavior which is critical for extrusion (
The shear recovery curves (
Mechanical properties of the bioprinted cartilaginous structures were assessed in tension. Tensile dumbbell specimens were printed using Bioink+HA particles with or without cells. The nozzle path (printing direction) in the gage section of the specimen was chosen to be parallel to the direction of tension (
Swelling of the bioink with and without particles was quantified to assess the total water retention and the water retention after gel crosslinking (
Cellular bioprinting process was investigated with Bioink+HA to exclude all the interactions and proliferation cues between particles and cells. One layer thick discs were printed to assess the cell viability after printing (
The effect of cartilage particles and growth factor, in this case TGF-β3, supplementation on cell proliferation was evaluated in casted gels cultured for 21 days. The Bioink alone did not stimulate cell proliferation; in fact there was a loss in DNA at day 7 which slowly recovered. Bioink+Cartilage particles, on the other hand, stimulated proliferation and caused a statistically significant increase (p<0.001) in DNA over 21 days. With TGF-β3 supplementation, there was a statistically significant increase in DNA in the Cartilage particles containing samples at day 7 (p<0.001). By day 21, both bioinks showed increases in DNA, which were not statistically significantly from each other.
Cartilage extracellular matrix production was evaluated in bioink alone and bioink+Cartilage particles with histology and immunostaining after 3 and 8 weeks in culture. Histological evaluation after 3 weeks revealed a clear increase in cell number, GAG synthesis and collagen II production in both bioink compositions supplemented with TGF-β3 (10 ng/ml). Furthermore, Bioink+Cartilage particles without growth factors stimulated cell proliferation above Bioink alone which was clearly visible with 3 and 8 week H&E staining. At both time points the Bioink+Cartilage particles showed a slight increase in Alcian blue staining and at the 8 week time point a slight collagen II staining was observed suggesting the need for additional growth factor stimulation. Cells were often seen proliferating around the particles without the growth factor supplementation which suggests that cell-particle adhesion and/or growth factors in the particles are important. However, because in the Bioink+Cartilage particles with TGF-β3 samples, no site-specific proliferation was observed, the results suggest rather the particles are a source of mitogenic growth factors and not specific cell-matrix adhesive cues. After 8 weeks, the gross appearance of the scaffolds suggested growth factor stimulation had a clear effect on cartilage matrix production as opaque appearance and increase in size was observed. At 8 weeks, both supplemented bioink compositions showed a significant increase in cartilage ECM components and had areas which began to resemble the cell density and GAG content of native cartilage. Furthermore, collagen II deposition was strong throughout the graft in the growth factor supplemented conditions while only pericellular staining was seen in the samples cultured without TGF-β3. Collagen I was found in Bioink+Cartilage particles and in both TGF-β3 supplemented conditions suggesting some fibrocartilage production, perhaps due to the passaging of the cells. In all the conditions calcification was absent suggesting the cartilage phenotype of the chondrocytes was stable.
To assess the shape retention of the printed structures several MRI techniques were evaluated. The printed nose was kept in PBS for 2 weeks to assure complete swelling prior T2-weighted MR imaging. These images were thresholded and converted into a .STL file and compared to the original model used for printing and to the cartilaginous graft immediately after printing. Comparison of the original model and the printed graft illustrates precise material extrusion and detailed structures. However, slightly thicker nostril walls were observed in comparison to the original model. Furthermore, when comparing the printed structure to the MRI model after 2 weeks swelling, a slight thickening of the nostril walls were observed, however, no sign of degradation or deterioration of the shape was detected.
One important factor of the reproducible printing process is the connectivity of the consecutive lines. In order to assess the effect of line spacing an optimization of line thickness must be conducted. Printing parameters such as pressure, feed rate and needle diameter were tested to standardize the line thickness to 900 μm±53 μm. After the determination of the average line thickness the effective line-line adhesion was investigated by printing a series of tensile testing dumbbells having different line spacing. The dumbbells were tensile tested until failure and the data illustrates that by increasing the line spacing the possibility of defects in the structure increased suggesting that in order to provide reproducible mechanical properties for printed structures the lines should overlap approximately 40-50%. The data suggested that the variance of the ultimate stress at failure did not differ in the tested samples with amount of overlapping lines down to 20% whereas the number of samples that were not stabile enough for testing increased with increasing line spacing. According to the data the optimal line spacing is affected by the bioink in question however by increasing the overlapping the probability of internal printing process related defects decreases. Furthermore the line thickness can be freely chosen by changing the process parameters such as pressure, printing speed and needle diameter.
Several mechanical testing measurements were performed for the newly designed bioink to investigate the parameters affecting the reproducibility of the structural and mechanical properties. The tensile evaluation of specimens printed with varying printing directions and with cell laden bioink revealed that the youngs modulus, ultimate stress and the failure strain are not altered by adding of the cells in the seeding density of 4×106 illustrating that the volume fraction of cells (˜1% approx.) is compensated by the strong surrounding matrix. Furthermore, dumbbell specimens were printed in varying printing directions with respect to the tension, namely parallel to tension (0°), perpendicular to tension(90°) and in 45° angle to the tension (45°). The printing direction did not show any statistically significant differences between the groups suggesting that the bioprinted structures can be designed based on the printing and process related parameters rather than based on the estimated mechanical loading of the final structures.
| Number | Date | Country | Kind |
|---|---|---|---|
| 14197449 | Dec 2014 | EP | regional |
| 15158224 | Mar 2015 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2015/079502 | 12/11/2015 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2016/092106 | 6/16/2016 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 6126690 | Ateshian | Oct 2000 | A |
| 7531503 | Atala | May 2009 | B2 |
| 20120089238 | Kang | Apr 2012 | A1 |
| Number | Date | Country |
|---|---|---|
| 2013204780 | Oct 2014 | AU |
| 2011119059 | Sep 2011 | WO |
| 2014032748 | Mar 2014 | WO |
| Entry |
|---|
| James et al. Differential Effects of Transforming Growth Factor-beta1 and -beta3 on Chondrogenesis in Posterofrontal Cranial Suture-Derived Mesenchymal Cells in Vitro. Plast Reconstr Surg. Jan. 2009 ; 123(1): 31-43. (Year: 2009). |
| Ferris et al. Modified gellan gum hydrogels for tissue engineering applications. Soft Matter, 9 (14), 3705-3711. (Year: 2013). |
| Lee et al. Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering. Journal of Biomedical Materials Research B: Applied Biomaterials. Aug. 2011 vol. 98B, Issue 2. p. 238-245 (Year: 2011). |
| Number | Date | Country | |
|---|---|---|---|
| 20170348458 A1 | Dec 2017 | US |
